Peptides and compounds that bind to a receptor

Abstract

Described are peptides and peptide mimetics that bind to and activate the thrombopoietin receptor. Such peptides and peptide mimetics are useful in methods for treating hematological disorders and particularly, thrombocytopenia resulting from chemotherapy, radiation therapy, or bone marrow transfusions as well as in diagnostic methods employing labeled peptides and peptide mimetics.

Description

BACKGROUND OF THE INVENTION

The present invention provides peptides and compounds that bind to and activate the thrombopoietin receptor (c-mpl or TPO-R) or otherwise act as a TPO agonist. The invention has application in the fields of biochemistry and medicinal chemistry and particularly provides TPO agonists for use in the treatment of human disease.

Megakaryocytes are bone marrow-derived cells, which are responsible for producing circulating blood platelets. Although comprising<0.25% of the bone marrow cells in most species, they have>10 times the volume of typical marrow cells. See Kuter, et. al., Proc. Natl. Acad. Sci. USA 91:11104-11108 (1994). Megakaryocytes undergo a process known as endomitosis whereby they replicate their nuclei but fail to undergo cell division and thereby give rise to polyploid cells. In response to a decreased platelet count, the endomitotic rate increases, higher ploidy megakaryocytes are formed, and the number of megakaryocytes may increase up to 3-fold. See Harker, J. Clin. Invest., 47:458-465 (1968). In contrast, in response to an elevated platelet count, the endomitotic rate decreases, lower ploidy megakaryocytes are formed, and the number of megakaryocytes may decrease by 50%.

The exact physiological feedback mechanism by which the mass of circulating platelets regulates the endomitotic rate and number of bone marrow megakaryocytes is not known. The circulating thrombopoietic factor involved in mediating this feedback loop is now thought to be thrombopoietin (TPO). More specifically, TPO has been shown to be the main humoral regulator in situations involving thrombocytopenia. See, e.g., Metcalf, Nature, 369:519-520 (1994). TPO has been shown in several studies to increase platelet counts, increase platelet size, and increase isotope incorporation into platelets of recipient animals. Specifically, TPO is thought to affect megakaryocytopoiesis in several ways: (1) it produces increases in megakaryocyte size and number; (2) it produces an increase in DNA content, in the form of polyploidy, in megakaryocytes; (3) it increases megakaryocyte endomitosis; (4) it produces increased maturation of megakaryocytes; and (5) it produces an increase in the percentage of precursor cells, in the form of small acetylcholinesterase-positive cells, in the bone marrow.

Because platelets (thrombocytes) are necessary for blood clotting and when their numbers are very low a patient is at serious risk of death from catastrophic hemorrhage, TPO has potential useful application in both the diagnosis and the treatment of various hematological disorders, for example, diseases primarily due to platelet defects. Ongoing clinical trials with TPO have indicated that TPO can be administered safely to patients. In addition, recent studies have provided a basis for the projection of efficacy of TPO therapy in the treatment of thrombocytopenia, and particularly thrombocytopenia resulting from chemotherapy, radiation therapy, or bone marrow transplantation as treatment for cancer or lymphoma. See, e.g., McDonald, Am. J. Ped. Hematology/Oncology, 14:8-21 (1992).

The gene encoding TPO has been cloned and characterized. See Kuter, et al., Proc. Natl. Acad. Sci. USA, 91:11104-11108 (1994); Barley, et al., Cell 77:1117-1124 (1994); Kaushansky et al., Nature 369:568-571 (1994); Wendling, et al., Nature, 369:571-574 (1994); and Sauvage et al., Nature 369:533-538 (1994). Thrombopoietin is a glycoprotein with at least two forms, with apparent molecular masses of 25 kDa and 31 kDa, with a common N-terminal amino acid sequence. See, Bartley, et al., Cell, 77:1117-1124 (1994). Thrombopoietin appears to have two distinct regions separated by a potential Arg-Arg cleavage site. The amino-terminal region is highly conserved in man and mouse, and has some homology with erythropoietin and interferon-a and interferon-b. The carboxy-terminal region shows wide species divergence.

The DNA sequences and encoded peptide sequences for human TPO-R (also known as c-mpl) have been described. See Vigon, et al., Proc. Natl. Acad. Sci. USA, 89:5640-5644 (1992). TPO-R is a member of the hematopoietin growth factor receptor family, a family characterized by a common structural design of the extracellular domain, including four conserved C residues in the N-terminal portion and a WSXWS motif close to the transmembrane region. See Bazan, Proc. Natl. Acad. Sci. USA, 87:6934-6938 (1990). Evidence that this receptor plays a functional role in hematopoiesis includes observations that its expression is restricted to spleen, bone marrow, or fetal liver in mice (see Souyri, et al., Cell 63:1137-1147 (1990)) and to megakaryocytes, platelets, and CD34 + cells in humans (see Methia, et al., Blood 82:1395-1401 (1993)). Furthermore, exposure of CD34 + cells to synthetic oligonucleotides antisense to mpl RNA significantly inhibits the appearance of megakaryocyte colonies without affecting erythroid or myeloid colony formation. Some workers postulate that the receptor functions as a homodimer, similar to the situation with the receptors for G-CSF and erythropoietin.

The availability of cloned genes for TPO-R facilitates the search for agonists of this important receptor. The availability of the recombinant receptor protein allows the study of receptor-ligand interaction in a variety of random and semi-random peptide diversity generation systems. These systems include the “peptides on plasmids” system described in U.S. Pat. Nos. 5,270,170 and 5,338,665; the “peptides on phage” system described in U.S. patent application Ser. No. 07/718,577, filed Jun. 20, 1991, U.S. patent application Ser. No. 07/541,108, filed Jun. 20, 1990, and in Cwirla, et al., Proc. Natl. Acad. Sci. USA, 87:6378-6382 (1990); the “polysome” system described in U.S. patent application Ser. No. 08/300,262, filed Sep. 2, 1994, which is a continuation-in-part application based on U.S. patent application Ser. No. 08/144,775, filed Oct. 29, 1993 and PCT WO 95/11992; the “encoded synthetic library” system described in U.S. patent application Ser. Nos. 08/146,886, filed Nov. 12, 1993, 07/946,239, filed Sep. 16, 1992, and 07/762,522, filed Sep. 18, 1991; and the “very large scale immobilized polymer synthesis” system described in U.S. Pat. No. 5,143,854; PCT Patent Publication No. 90/15070, published Dec. 13, 1990; U.S. patent application Ser. No. 07/624,120, filed Dec. 6, 1990; Fodor, et al., Science, 251:767-773 (2/1991); Dower, et al., Ann. Rep. Med. Chem., 26:271-180 (1991); and U.S. patent application Ser. No. 07/805,727, filed Dec. 6, 1991; each of the foregoing patent applications and publications is incorporated herein by reference.

The slow recovery of platelet levels in patients suffering from thrombocytopenia is a serious problem, and has lent urgency to the search for a blood growth factor agonist able to accelerate platelet regeneration. The present invention provides such an agonist.

SUMMARY OF THE INVENTION

This invention is directed, in part, to the novel and unexpected discovery that defined low molecular weight peptides and peptide mimetics have strong binding properties to the TPO-R and can activate the TPO-R. Accordingly, such peptides and peptide mimetics are useful for therapeutic purposes in treating conditions mediated by TPO (e.g., thrombocytopenia resulting from chemotherapy, radiation therapy, or bone marrow transfusions) as well as for diagnostic purposes in studying the mechanism of hematopoiesis and for the in vitro expansion of megakaroycytes and committed progenitor cells.

Peptides and peptide mimetics suitable for therapeutic and/or diagnostic purposes have an IC 50 of about 2 mM or less, as determined by the binding affinity assay set forth in Example 3 below wherein a lower IC 50 correlates to a stronger binding affinity to TPO-R. For pharmaceutical purposes, the peptides and peptidomimetics preferably have an IC 50 of no more than about 100 μm, more preferably, no more than 500 nM. In a preferred embodiment, the molecular weight of the peptide or peptide mimetic is from about 250 to about 8,000 daltons. If the peptides of this invention are oligomerized, dimerized and/or derivatized with a hydrophilic polymer as described herein, the molecular weights of such peptides will be substantially greater and can range anywhere from about 500 to about 120,000 daltons, more preferable from about 8,000 to about 80,000 daltons.

When used for diagnostic purposes, the peptides and peptide mimetics preferably are labeled with a detectable label and, accordingly, the peptides and peptide mimetics without such a label serve as intermediates in the preparation of labeled peptides and peptide mimetics.

Peptides meeting the defined criteria for molecular weight and binding affinity for TPO-R comprise 9 or more amino acids wherein the amino acids are naturally occurring or synthetic (non-naturally occurring) amino acids. Peptide mimetics include peptides having one or more of the following modifications:

peptides wherein one or more of the peptidyl [—C(O)NR—] linkages (bonds) have been replaced by a non-peptidyl linkage such as a —CH 2 -carbamate linkage [—CH 2 —OC(O)NR—]; a phosphonate linkage; a —CH 2 -sulfonamide [—CH 2 —S(O) 2 NR—] linkage; a urea [—NHC(O)NH—] linkage; a —CH 2 -secondary amine linkage; or an alkylated peptidyl linkage [—C(O)NR 6 — where R 6 is lower alkyl];

peptides wherein the N-terminus is derivatized to a —NRR 1 group; to a —NRC(O)R group; to a —NRC(O)OR group; to a —NRS(O) 2 R group; to a —NHC(O)NHR group where R and R 1 are hydrogen or lower alkyl with the proviso that R and R 1 are not both hydrogen; to a succinimide group; to a benzyloxycarbonyl-NH—(CBZ—NH—) group; or to a benzyloxycarbonyl-NH— group having from 1 to 3 substituents on the phenyl ring selected from the group consisting of lower alkyl, lower alkoxy, chloro, and bromo; or

peptides wherein the C terminus is derivatized to —C(O)R 2 where 2 is selected from the group consisting of lower alkoxy, and —NR 3 R 4 where R 3 and R 4 are independently selected from the group consisting of hydrogen and lower alkyl.

Accordingly, preferred peptides and peptide mimetics comprise a compound having:

(1) a molecular weight of less than about 5000 daltons, and

(2) a binding affinity to TPO-R as expressed by an IC 50 of no more than about 100 μm,

wherein from zero to all of the —C(O)NH— linkages of the peptide have been replaced by a linkage selected from the group consisting of a —CH 2 OC(O)NR— linkage; a phosphonate linkage; a —CH 2 S(O) 2 NR— linkage; a —CH 2 NR— linkage; and a —C(O)NR 6 — linkage; and a —NHC(O)NH— linkage where R is hydrogen or lower alkyl and R 6 is lower alkyl,

further wherein the N-terminus of said peptide or peptide mimetic is selected from the group consisting of a —NRR 1 group; a —NRC(O)R group; a —NRC(O)OR group; a —NRS(O) 2 R group; a —NHC(O)NHR group; a succinimide group; a benzyloxycarbonyl-NH— group; and a benzyloxycarbonyl-NH— group having from 1 to 3 substituents on the phenyl ring selected from the group consisting of lower alkyl, lower alkoxy, chloro, and bromo, where R and R 1 are independently selected from the group consisting of hydrogen and lower alkyl,

and still further wherein the C-terminus of said peptide or peptide mimetic has the formula —C(O)R 2 where R 2 is selected from the group consisting of hydroxy, lower alkoxy, and —NR 3 R 4 where R 3 and R 4 are independently selected from the group consisting of hydrogen and lower alkyl and where the nitrogen atom of the —NR 3 R 4 group can optionally be the amine group of the N-terminus of the peptide so as to form a cyclic peptide,

and physiologically acceptable salts thereof.

In a related embodiment, the invention is directed to a labeled peptide or peptide mimetic comprising a peptide or peptide mimetic described as above having covalently attached thereto a label capable of detection.

In some embodiments of the invention, preferred peptides for use include peptides having a core structure comprising a sequence of amino acids:

X 1 X 2 X 3 X 4 X 5 X 6 X 7

where X 1 is C, L, M, P, Q, V; X 2 is F, K, L, N, Q, R, S, T or V; X 3 is C, F, I, L, M, R, S, V or W; X 4 is any of the 20 genetically coded L-amino acids; X 5 is A, D, E, G, K, M, Q, R, S, T, V or Y; X 6 is C, F, G, L, M, S, V, W or Y; and X 7 is C, G, I, K, L, M, N, R or V.

In a preferred embodiment the core peptide comprises a sequence of amino acids:

X 8 G X 1 X 2 X 3 X 4 X 5 W X 7

where X 1 is L, M, P, Q, or V; X 2 is F, R, S, or T; X 3 is F, L, V, or W; X 4 is A, K, L, M, R, S, V, or T; X 5 is A, E, G, K, M, Q, R, S, or T; X 7 is C, I, K, L, M or V; and each X 8 residue is independently selected from any of the 20 genetically coded L-amino acids, their stereoisomeric D-amino acids; and non-natural amino acids. Preferably, each X 8 residue is independently selected from any of the 20 genetically coded L-amino acids and their stereoisomeric D-amino acids. In a preferred embodiment, X 1 is P; X 2 is T; X 3 is L; X 4 is R; X 5 is E or Q; and X 7 is I or L.

More preferably, the core peptide comprises a sequence of amino acids:

X 9 X 8 G X 1 X 2 X 3 X 4 X 5 W X 7

where X 9 is A, C, E; G, I; L, M, P, R, Q, S, T, or V; and X 8 . is A, C, D, E, K, L, Q, R, S, T, or V. More preferably, X 9 is A or I; and X 8 is D, E, or K.

Particularly preferred peptides include: G G C A D G P T L R E W I S F C G G; G N A D G P T L R Q W L E G R R P K N; G G C A D G P T L R E W I S F C G G K; T I K G P T L R Q W L K S R E H T S; S I E G P T L R E W L T S R T P H S; L A I E G P T L R Q W L H G N G R D T; C A D G P T L R E W I S F C; and I E G P T L R Q W L A A R A.

In further embodiments of the invention, preferred peptides for use in this invention include peptides having a core structure comprising a sequence of amino acids:

 C X 2 X 3 X 4 X 5 X 6 X 7

where X 2 is F, K, L, N, Q. R, S, T or V; X 3 is C, F, I, L, M, R, S or V; X 4 is any of the 20 genetically coded L-amino acids; X 5 is A, D, E, G, S, V or Y; X 6 is C, F, G, L, M, S, V, W or Y; and X 7 is C, G, I, K, L, M, N, R or V. In a more preferred embodiment, X 4 is A, E, G, H, K, L, M, P, Q, R, S, T, or W. In a further embodiment, X 2 is S or T; X 3 is L or R; X 4 is R; X 5 is D, E, or G; X 6 is F, L, or W; and X 7 is I, K, L, R, or V. Particularly preferred peptides include: G G C T L R E W L H G G F C G G.

In a further embodiment, preferred peptides for use in this invention include peptides having a structure comprising a sequence of amino acids:

X 8 C X 2 X 3 X 4 X 5 X 6 X 7

where X 2 is F, K, L, N, Q, R, S, T or V; X 3 is C, F, I, L, M, R, S, V or W; X 4 is any of the 20 genetically coded L-amino acids; X 5 is A, D, E, G, K, M, Q, R, S, T, V or Y; X 6 is C, F, G, L, M, S, V, W or Y; X 7 is C, G, I, K, L, M, N, R or V; and X 8 is any of the 20 genetically coded L-amino acids. In some embodiments, X 8 is preferably G, S, Y, or R.

In another embodiment, the peptide compounds of the present invention are preferably dimerized or oligomerized to increase the affinity and/or activity of the compounds. Examples of preferred dimerized peptide compounds include, but are not limited to, the following:

In yet a further embodiment, preferred peptides for use in this invention include peptides that are covalently attached to one or more of a variety of hydrophilic polymers. Suitable hydrophilic polymers include, but are not limited to, polyalkylethers as exemplified by polyethylene glycol and polypropylene glycol, polylactic acid, polyglycolic acid, polyoxyalkenes, polyvinylalcohol, polyvinylpyrrolidone, cellulose and cellulose derivatives, dextran and dextran derivatives, etc. It has surprisingly been discovered that when the peptide compounds are derivatized with such polymers, their solubility and circulation half-lives are increased with little, if any, diminishment in their binding activity. In a presently preferred embodiment, the peptide compounds of this invention are dimerized and each of the dimeric subunits is covalently attached to a hydrophilic polymer. In a further preferred embodiment, the peptide compounds of this invention are PEGylated, i.e., covalently attached to polyethylene glycol (PEG). Examples of preferred PEGylated, dimerized peptide compositions of this invention include, but are not limited to, the following:

wherein “n” is an interger having a value ranging from about 5 to about 1000, more preferably from about 10 to about 600 and, even more preferably, from about 110 to about 450.

The compounds described herein are useful for the prevention and treatment of diseases mediated by TPO, and particularly for treating hematological disorders, including but not limited to, thrombocytopenia resulting from chemotherapy, radiation therapy, or bone marrow transfusions. Thus, the present invention also provides a method for treating wherein a patient having a disorder that is susceptible to treatment with a TPO agonist receives, or is administered, a therapeutically effective dose or amount of a compound of the present invention.

The invention also provides for pharmaceutical compositions comprising one or more of the compounds described herein and a physiologically acceptable carrier. These pharmaceutical compositions can be in a variety of forms including oral dosage forms, as well as inhalable powders and solutions and injectable and infusible solutions.

BRIEF DESCRIPTION OF THE FIGURES

FIGS. 1A-B illustrates the results of a functional assay in the presence of various peptides; the assay is described in Example 2.

FIG. 1A is a graphical depiction of the results of the TPO-R transfected Ba/F3 cell proliferation assay for selected peptides of the invention:

▪ designating the results for G G C A D G P T L R E W I S F C G G K (biotin);

X designating the results for G G C A D G P T L R E W I S F C G G;

▴ designating the results for L A I E G P T L R Q W L H G N G R D T;

∘ designating the results for G N A D G P T L R Q W L E G R R P K N; and

+ designating the results for T I K G P T L R Q W L K S R E H T S.

FIG. 1B is a graphical depiction of the results with the same peptides and the parental cell line.

FIGS. 2A-C show the results of peptide oligomerization using the TPO-R transfected Ba/F3 cell proliferation assay.

FIG. 2A shows the results of the assay for the complexed biotinylated peptide (AF 12285 with streptavidin (SA)) for both the transfected and parental cell lines.

FIG. 2B shows the results of the assay for the free biotinylated peptide (AF 12285) for both the transfected and parental cell lines.

FIG. 2C shows the results of the assay for streptavidin alone for both the transfected and parental cell lines.

FIGS. 3A-G show the results of a series of control experiments showing the activity of TPO, the peptides of the present invention, EPO, and EPO-R binding peptides in a cell proliferation assay using either the TPO-R transfected Ba/F3 cell line and its corresponding parental line, or an EPO-dependent cell line.

FIG. 3A depicts the results for TPO in the cell proliferation assay using the TPO-R transfected Ba/F3 cell line and its corresponding parental line.

FIG. 3B depicts the results for EPO in the cell proliferation assay using the TPO-R transfected Ba/F3 cell line and its corresponding parental line.

FIG. 3C depicts the results for complexed biotinylated peptide (AF 12285 with streptavidin (SA)) and a complexed form of a biotinylated EPO-R binding peptide (AF 11505 with SA) in the TPO-R transfected Ba/F3 cell line. The results for the corresponding parental cell line are shown in FIG. 3 D.

FIG. 3E depicts the results for TPO in the cell proliferation assay using the EPO-dependent cell line.

FIG. 3F depicts the results for EPO in the cell proliferation assay using the EPO-dependent cell line.

FIG. 3G depicts the results for complexed biotinylated peptide (AF 12885 with streptavidin (SA)) and the complexed form of a biotinylated EPO-R binding peptide (AF 11505 with SA) in the EPO-dependent cell line.

FIGS. 4A-C illustrates the construction of peptides-on-plasmids libraries in vector pJS142.

FIG. 4A shows a restriction map and position of the genes. The library plasmid includes the rrnb transcriptional terminator, the bla gene to permit selection on ampicillin, the M13 phage intragenic region (M13 IG) to permit rescue of single-stranded DNA, a plasmid replication origin (ori), two lacO s sequences, and the araC gene to permit positive and negative regulation of the araB promoter driving expression of the lac fusion gene.

FIG. 4B shows the sequence of the cloning region at the 3′ end of the lac I gene, including the SfiI and EagI sites used during library construction.

FIG. 4C shows the ligation of annealed library oligonucleotides, ON-829 and ON-830, to SfiI sites of pJS142 to produce a library. Single spaces in the sequence indicate sites of ligation.

FIGS. 5A-B illustrate cloning into the pELM3 and pELM15 MBP vectors.

FIG. 5A shows the sequence at the 3′ end of the malE fusion gene, including the MBP coding sequence, the poly asparagine linker, the factor Xa protease cleavage site, and the available cloning sites. The remaining portions of the vectors are derived from pMALc2 (pELM3) and pMALp2 (pELM15), available from New England Biolabs. FIG. 5B shows the sequence of the vectors after transfer of the BspEII-ScaI library fragment into AgeI-ScaI digested pELM3/pELM15. The transferred sequence includes the sequence encoding the GGG peptide linker from the pJS142 library.

FIG. 6A depicts a restriction map and position of the genes for the construction of headpiece dimer libraries in vector pCMG14. The library plasmid includes: the rrnB transcriptional terminator, the bla gene to permit selection on ampicillin, the M13 phage intragenic region (M13 IG) to permit rescue of single-stranded DNA, a plasmid replication origin (ori), one lacO s sequence, and the araC gene to permit positive and negative regulation of the araB promoter driving expression of the headpiece dimer fusion gene.

FIG. 6B depicts the sequence of the cloning region at the 3′ end of the headpiece dimer gene, including the SfiI and EagI sites used during library construction.

FIG. 6C shows the ligation of annealed ON-1679, ON-829, and ON-830 to SfiI sites of pCMG14 to produce a library. Singles spaces in the sequence indicate sites of ligation.

FIGS. 7-9 show the results of further assays evaluating activity of the peptides and peptide mimetics of the invention. In this assay mice are made thrombocytopenic with carboplatin.

FIG. 7 depicts typical results when Balb/C mice are treated with carboplatin (125 mg/kg intraperitoneally) on Day 0. The dashed lines represent untreated animals from three experiments. The solid line represent carboplatin-treated groups in three experiments. The heavy solid lines represent historical data.

FIG. 8 depicts the effect of carboplatin titration on platelet counts in mice treated with the indicated amounts of carboplatin (in mg/kg, intraperitoneally (ip) on Day 0).

FIG. 9 depicts amelioration of carboplatin-induced thrombocytopenia on Day 10 by peptide AF12513 (513). Carboplatin (CBP; 50-125 mg/kg, intraperitoneally) was administered on Day 0. AF12513 (1 mg/kg, ip) was given on Days 1-9.

FIG. 10 illustrates the plasma concentration of 5K PEG-AF13948, 20K PEG-AF13948 and vehicle AF13948, 5K PEG-AF13948 and vehicle following IV administration of 700 μg/kg in mice.

FIG. 11 illustrates the plasma concentration of 5K PEG-AF13948, 20K PEG AF13948 and vehicle following IV administration of 500 μg/kg in mice.

FIG. 12 illustrates the plasma concentrations of 20K DiPEG-AF13948 following IV injection of 100, 10, and 1 μg/kg in mice.

FIG. 13 illustrates the plasma concentration of AF13948-20K dePEG following IV and SC administration of 10 μg/kg to mice. An can be nee from FIGS. 10-13 , PEGylation of the peptide compound results in their improved pharmacokinetic characteristics.

FIG. 14 illustrates the stability of AF13948 and GW350781 in human serum and demonstrates that the PEGylated compound has increased stability, i.e., increased half-life, over the non-PEGylated compound.

FIGS. 15-17 illustrate exemplar reactions schemes for derivatizing the peptide compound of the present invention with, for example, polyethylene glycol (PEG).

FIG. 18 illustrates the pharmacokinetic profiles of a peptide compound of the present invention variously derivatized with PEG. In this experiment, the peptide AF13948 was derivatized with branched PEG (di(2)), with an ester linked PEG (SPA) and with an aldehyde linked PEG (ALDH) (see, FIGS. 15 - 17 ). The results obtained indicate that all three of the peptide compounds variously derivatized with PEG have favorable pharmacokinetic profiles.

FIGS. 19-20 illustrate the effects of the PEGylated peptide compounds of the present invention on carboplatin (CBP)-induced thrombocytopenia in mice.

FIG. 19 demonstrates that GW350781, the 5K-PEGylated peptide, can ameliorate thrombocytopenia in a mouse model.

FIG. 20 demonstrates that GW305805, the 20K-diPEGylated peptide, can also ameliorate carboplatin-induced thrombocytopenia and that it is even more potent than the 5K-PEGylated peptide.

FIGS. 21-22 illustrate the effects of CW350781 and GW350805 on thrombocytosis in normal mice. The results obtained indicate that the PEGylated peptide compounds of the invention have a favorable effect thrombocytosis, with the 20K-diPEGylated peptide being about 100-fold more potent than the 5K-PEGylated peptide.

FIGS. 23-24 illustrate the effects of varying doses of GW350781 and GW350805 on platelet levels in normal mice. Such data demonstrate that the PEGylated peptide compounds of the invention can increase platelet levels in normal mice.

FIG. 25 illustrates the effect of single-dose vs. multiple-dose of GW305805 on platelet levels in normal mice.

FIG. 26 illustrates a general synthetic scheme for making dimer peptides with β-Ala.

FIG. 27 illustrates a general synthetic scheme for making dimer peptides without β-Ala.

DESCRIPTION OF SPECIFIC EMBODIMENTS

I. Definitions and General Parameters

The following definitions are set forth to illustrate and define the meaning and scope of the various terms used to describe the invention herein.

“Agonist” refers to a biologically active ligand which binds to its complementary biologically active receptor and activates the latter either to cause a biological response in the receptor or to enhance preexisting biological activity of the receptor.

“Pharmaceutically acceptable salts” refer to the non-toxic alkali metal, alkaline earth metal, and ammonium salts commonly used in the pharmaceutical industry including the sodium, potassium, lithium, calcium, magnesium, barium, ammonium, and protamine zinc salts, which are prepared by methods well known in the art. The term also includes non-toxic acid addition salts, which are generally prepared by reacting the compounds of this invention with a suitable organic or inorganic acid. Representative salts include the hydrochloride, hydrobromide, sulfate, bisulfate, acetate, oxalate, valerate, oleate, laurate, borate, benzoate, lactate, phosphate, tosylate, citrate, maleate, fumarate, succinate, tartrate, napsylate, and the like.

“Pharmaceutically acceptable acid addition salt” refers to those salts which retain the biological effectiveness and properties of the free bases and which are not biologically or otherwise undesirable, formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, and organic acids such as acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, malic acid, malonic acid, succinic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, menthanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid and the like. For a description of pharmaceutically acceptable acid addition salts as prodrugs, see Bundgaard, H., supra.

“Pharmaceutically acceptable ester” refers to those esters which retain, upon hydrolysis of the ester bond, the biological effectiveness and properties of the carboxylic acid or alcohol and are not biologically or otherwise undesirable. For a description of pharmaceutically acceptable esters as prodrugs, see Bundgaard, H., ed., Design of Prodrugs, Elsevier Science Publishers, Amsterdam (1985). These esters are typically formed from the corresponding carboxylic acid and an alcohol. Generally, ester formation can be accomplished via conventional synthetic techniques. (See, e.g., March, Advanced Organic Chemistry, 4th Ed., John Wiley & Sons, New York (1992), 393-396 and references cited therein, and Mark, et al., Encyclopedia of Chemical Technology, John Wiley & Sons, New York (1980).) The alcohol component of the ester will generally comprise (i) a C 2 -C 12 aliphatic alcohol that can or can not contain one or more double bonds and can or can not contain branched carbons or (ii) a C 7 -C 12 aromatic or heteroaromatic alcohols. This invention also contemplates the use of those compositions which are both esters as described herein and at the same time are the pharmaceutically acceptable acid addition salts thereof.

“Pharmaceutically acceptable amide” refers to those amides which retain, upon hydrolysis of the amide bond, the biological effectiveness and properties of the carboxylic acid or amine and are not biologically or otherwise undesirable. For a description of pharmaceutically acceptable amides as prodrugs, see Bundgaard, H., ed., Design of Prodrugs, Elsevier Science Publishers, Amsterdam (1985). These amides are typically formed from the corresponding carboxylic acid and an amine. Generally, amide formation can be accomplished via conventional synthetic techniques. (See, e.g., March, Advanced Organic Chemistry, 4th Ed., John Wiley & Sons, New York (1992), p. 393 and Mark, et al. Encyclopedia of Chemical Technology, John Wiley & Sons, New York (1980).) This invention also contemplates the use of those compositions which are both amides as described herein and at the same time are the pharmaceutically acceptable acid addition salts thereof.

“Pharmaceutically or therapeutically acceptable carrier” refers to a carrier medium which does not interfere with the effectiveness of the biological activity of the active ingredients and which is not toxic to the host or patient.

“Stereoisomer” refers to a chemical compound having the same molecular weight, chemical composition, and constitution as another, but with the atoms grouped differently. That is, certain identical chemical moieties are at different orientations in space and, therefore, when pure, has the ability to rotate the plane of polarized light. However, some pure stereoisomers may have an optical rotation that is so slight that it is undetectable with present instrumentation. The compounds of the instant invention may have one or more asymmetrical carbon atoms and therefore include various stereoisomers. All stereoisomers are included within the scope of the invention.

“Therapeutically- or pharmaceutically-effective amount” as applied to the compositions of the instant invention refers to the amount of composition sufficient to induce a desired biological result. That result can be alleviation of the signs, symptoms, or causes of a disease, or any other desired alteration of a biological system. In the present invention, the result will typically involve a decrease in the immunological and/or inflammatory responses to infection or tissue injury.

Amino acid residues in peptides are abbreviated as follows: Phenylalanine is Phe or F; Leucine is Leu or L; Isoleucine is Ile or I; Methionine is Met or M; Valine is Val or V; Serine is Ser or S; Proline is Pro or P; Threonine is Thr or T; Alanine is Ala or A; Tyrosine is Tyr or Y; Histidine is His or H; Glutamine is Gln or Q; Asparagine is Asn or N; Lysine is Lys or K; Aspartic Acid is Asp or D; Glutamic Acid is Glu or E; Cysteine is Cys or C; Tryptophan is Trp or W; Arginine is Arg or R; and Glycine is Gly or G. Additionally, t-Buo is tert-bulyloxy, Bzl is benzyl, CHA is cyclohexylamine, Ac is acetyl, Me is methyl, Pen is penicillamine, Aib is aminoisobutyric acid, Nva is norvaline, Abu is aminobutyric acid, Thi is thienylalanine, OBn is O-benzyl, and hyp is hydroxyproline.

In addition to peptides consisting only of naturally-occurring amino acids, peptidomimetics or peptide analogs are also provided. Peptide analogs are commonly used in the pharmaceutical industry as non-peptide drugs with properties analogous to those of the template peptide. These types of non-peptide compound are termed “peptide mimetics” or “peptidomimetics” (Luthman, et al., A Textbook of Drug Design and Development, 14:386-406, 2nd Ed., Harwood Academic Publishers (1996); Joachim Grante, Angew. Chem. Int. Ed. Engl., 33:1699-1720 (1994); Fauchere, J., Adv. Drug Res., 15:29 (1986); Veber and Freidinger TINS, p. 392 (1985); and Evans, et al., J. Med. Chem. 30:229 (1987), which are incorporated herein by reference). Peptide mimetics that are structurally similar to therapeutically useful peptides may be used to produce an equivalent or enhanced therapeutic or prophylactic effect. Generally, peptidomimetics are structurally similar to a paradigm polypeptide (i.e., a polypeptide that has a biological or pharmacological activity), such as naturally-occurring receptor-binding polypeptide, but have one or more peptide linkages optionally replaced by a linkage selected from the group consisting of: —CH 2 NH—, —CH 2 S—, —CH 2 —CH 2 —, —CH=CH—(cis and trans), —COCH 2 —, —CH(OH)CH 2 —, and —CH 2 SO—, by methods known in the art and further described in the following references: Spatola, A. F. in Chemistry and Biochemistry of Amino Acids, Peptides, and Proteins, B. Weinstein, eds., Marcel Dekker, New York, p. 267 (1983); Spatola, A. F., Vega Data (Mar. 1983), Vol. 1, Issue 3, Peptide Backbone Modifications (general review); Morley, Trends Pharm. Sci. pp. 463-468 (1980), (general review); Hudson, et al., Int. J. Pept. Prot. Res., 14:177-185 (1979) (—CH 2 NH—, CH 2 CH 2 —); Spatola, et al., Life Sci., 38:1243-1249 (1986) (—CH 2 —S); Hann, J. Chem. Soc. Perkin Trans. I, 307-314 (1982) (—CH—CH—, cis and trans); Almquist, et al., J. Med. Chem., 23:1392-1398, (1980) (—COCH 2 —); Jennings-White, et al., Tetrahedron Lett. 23:2533 (1982) (—COCH 2 —); Szelke, et al., European Appln. EP 45665 (1982) (—CH(OH)CH 2 —); Holladay, et al., Tetrahedron Lett., 24:4401-4404 (1983) (—C(OH)CH 2 —); and Hruby, Life Sci., 31:189-199 (1982) (—CH 2 —S—); each of which is incorporated herein by reference. A particularly preferred non-peptide linkage is —CH 2 NH—. Such peptide mimetics may have significant advantages over polypeptide embodiments, including, for example: more economical production, greater chemical stability, enhanced pharmacological properties (half-life, absorption, potency, efficacy, etc.), altered specificity (e.g., a broad-spectrum of biological activities), reduced antigenicity, and others. Labeling of peptidomimetics usually involves covalent attachment of one or more labels, directly or through a spacer (e.g., an amide group), to non-interfering position(s) on the peptidomimetic that are predicted by quantitative structure-activity data and/or molecular modeling. Such non-interfering positions generally are positions that do not form direct contacts with the macromolecules(s) (e.g., immunoglobulin superfamily molecules) to which the peptidomimetic binds to produce the therapeutic effect. Derivitization (e.g., labeling) of peptidomimetics should not substantially interfere with the desired biological or pharmacological activity of the peptidomimetic. Generally, peptidomimetics of receptor-binding peptides bind to the receptor with high affinity and possess detectable biological activity (i.e., are agonistic or antagonistic to one or more receptor-mediated phenotypic changes).

Systematic substitution of one or more amino acids of a consensus sequence with a D-amino acid of the same type (e.g., D-lysine in place of L-lysine) may be used to generate more stable peptides. In addition, constrained peptides comprising a consensus sequence or a substantially identical consensus sequence variation may be generated by methods known in the art (Rizo, et al., Ann. Rev. Biochem., 61:387 (1992), incorporated herein by reference); for example, by adding internal cysteine residues capable of forming intramolecular disulfide bridges which cyclize the peptide.

Synthetic or non-naturally occurring amino acids refer to amino acids which do not naturally occur in vivo but which, nevertheless, can be incorporated into the peptide structures described herein. Preferred synthetic amino acids are the D-α-amino acids of naturally occurring L-α-amino acid as well as non-naturally occurring D- and L-α-amino acids represented by the formula H 2 NCHR 5 COOH where R 5 is 1) a lower alkyl group, 2) a cycloalkyl group of from 3 to 7 carbon atoms, 3) a heterocycle of from 3 to 7 carbon atoms and 1 to 2 heteroatoms selected from the group consisting of oxygen, sulfur, and nitrogen, 4) an aromatic residue of from 6 to 10 carbon atoms optionally having from 1 to 3 substituents on the aromatic nucleus selected from the group consisting of hydroxyl, lower alkoxy, amino, and carboxyl, 5) -alkylene-Y where alkylene is an alkylene group of from 1 to 7 carbon atoms and Y is selected from the group consisting of (a) hydroxy, (b) amino, (c) cycloalkyl and cycloalkenyl of from 3 to 7 carbon atoms, (d) aryl of from 6 to 10 carbon atoms optionally having from 1 to 3 substituents on the aromatic nucleus selected from the group consisting of hydroxyl, lower alkoxy, amino and carboxyl, (e) heterocyclic of from 3 to 7 carbon atoms and 1 to 2 heteroatoms selected from the group consisting of oxygen, sulfur, and nitrogen, (f) —C(O)R 2 where R 2 is selected from the group consisting of hydrogen, hydroxy, lower alkyl, lower alkoxy, and —NR 3 R 4 where R 3 and R 4 are independently selected from the group consisting of hydrogen and lower alkyl, (g) —S(O) n R 6 where n is an integer from 1 to 2 and R 6 is lower alkyl and with the proviso that R 5 does not define a side chain of a naturally occurring amino acid.

Other preferred synthetic amino acids include amino acids wherein the amino group is separated from the carboxyl group by more than one carbon atom such as β-alanine, γ-aminobutyric acid, and the like.

Particularly preferred synthetic amino acids include, by way of example, the D-amino acids of naturally occurring L-amino acids, L-(1-naphthyl)-alanine, L-(2-naphthyl)-alanine, L-cyclohexylalanine, L-2-aminoisobutyric acid, the sulfoxide and sulfone derivatives of methionine (i.e., HOOC—(H 2 NCH)CH 2 CH 2 —S(O) n R 6 ) where n and R 6 are as defined above as well as the lower alkoxy derivative of methionine (i.e., HOOC—(H 2 NCH)CH 2 CH 2 —OR 6 where R 6 is as defined above).

“Detectable label” refers to materials, which when covalently attached to the peptides and peptide mimetics of this invention, permit detection of the peptide and peptide mimetics in vivo in the patient to whom the peptide or peptide mimetic has been administered. Suitable detectable labels are well known in the art and include, by way of example, radioisotopes, fluorescent labels (e.g., fluorescein), and the like. The particular detectable label employed is not critical and is selected relative to the amount of label to be employed as well as the toxicity of the label at the amount of label employed. Selection of the label relative to such factors is well within the skill of the art.

Covalent attachment of the detectable label to the peptide or peptide mimetic is accomplished by conventional methods well known in the art. For example, when the 125 I radioisotope is employed as the detectable label, covalent attachment of 125 I to the peptide or the peptide mimetic can be achieved by incorporating the amino acid tyrosine into the peptide or peptide mimetic and then iodimating the peptide (see, e.g., Weaner, et al., Synthesis and Applications of Isotopically Labelled Compounds, pp. 137-140 (1994)). If tyrosine is not present in the peptide or peptide mimetic, incorporation of tyrosine to the N or C terminus of the peptide or peptide mimetic can be achieved by well known chemistry. Likewise, 32 P can be incorporated onto the peptide or peptide mimetic as a phosphate moiety through, for example, a hydroxyl group on the peptide or peptide mimetic using conventional chemistry.

II. Overview

The present invention provides compounds that bind to and activate the TPO-R or otherwise behave as a TPO agonist. These compound include “lead” peptide compounds and “derivative” compounds constructed so as to have the same or similar molecular structure or shape as the lead compounds but that differ from the lead compounds either with respect to susceptibility to hydrolysis or proteolysis and/or with respect to other biological properties, such as increased affinity for the receptor. The present invention also provides compositions comprising an effective amount of a TPO agonist, and more particularly a compound, that is useful for treating hematological disorders, and particularly, thrombocytopenia associated with chemotherapy, radiation therapy, or bone marrow transfusions.

III. Identification of TPO-Agonists

Peptides having a binding affinity to TPO-R can be readily identified by random peptide diversity generating systems coupled with an affinity enrichment process.

Specifically, random peptide diversity generating system include the “peptides on plasmids” system described in U.S. Pat. Nos. 5,270,170 and 5,338,665; the “peptides on phage” system described in U.S. patent application Ser. No. 07/718,577, filed Jun. 20, 1991 which in a continuation in part application of U.S. patent application Ser. No. 07/541,108, filed Jun. 20, 1990, and in Cwirla, et al., Proc. Natl. Acad. Sci. USA, 87:6378-6382 (1990); the “polysome system” described in U.S. patent application Ser. No. 08/300,262, filed Sep. 2, 1994, which is a continuation-in-part application based on U.S. patent application Ser. No. 08/144,775, filed Oct. 29, 1993 and PCT WO 95/11992; the “encoded synthetic library (ESL)” system described in U.S. patent application Ser. No. 08/146,886, filed Nov. 12, 1993 which is a continuation in part application of U.S. patent application Ser. No. 07/946,239, filed Sep. 16, 1992, which is a continuation in part application of U.S. patent application Ser. No. 07/762,522, filed Sep. 18, 1991; and the “very large scale immobilized polymer synthesis” system described in U.S. Pat. No. 5,143,854; PCT Patent Publication No. 90/15070, published Dec. 13, 1990; U.S. patent application Ser. No. 07/624,120, filed Dec. 6, 1990; Fodor, et al., Science, 251:767-773 (February 1991); Dower, et al., Ann. Rep. Med. Chem., 26:271-180 (1991); and U.S. patent application Ser. No. 805,727, filed Dec. 6, 1991.

Using the procedures described above, random peptides were generally designed to have a defined number of amino acid residues in length (e.g., 12). To generate the collection of oligonucleotides encoding the random peptides, the codon motif (NNK)x, where N is nucleotide A, C, G, or T (equimolar; depending on the methodology employed, other nucleotides can be employed), K is G or T (equimolar), and x is an integer corresponding to the number of amino acids in the peptide (e.g., 12) was used to specify any one of the 32 possible codons resulting from the NNK motif: 1 for each of 12 amino acids, 2 for each of 5 amino acids, 3 for each of 3 amino acids, and only one of the three stop codons. Thus, the NNK motif encodes all of the amino acids, encodes only one stop codon, and reduces codon bias.

In the systems employed, the random peptides were presented either on the surface of a phage particle, as part of a fusion protein comprising either the pIII or the pVIII coat protein of a phage fd derivative (peptides on phage) or as a fusion protein with the LacI peptide fusion protein bound to a plasmid (peptides on plasmids).

The phage or plasmids, including the DNA encoding the peptides, were identified and isolated by an affinity enrichment process using immobilized TPO-R. The affinity enrichment process, sometimes called “panning,” involves multiple rounds of incubating the phage, plasmids, or polysomes with the immobilized receptor, collecting the phage, plasmids, or polysomes that bind to the receptor (along with the accompanying DNA or mRNA), and producing more of the phage or plasmids (along with the accompanying LacI-peptide fusion protein) collected. The extracellular domain (ECD) of the TPO-R typically was used during panning.

After several rounds of affinity enrichment, the phage or plasmids and accompanying peptides were examined by ELISA to determine if the peptides bind specifically to TPO-R. This assay was carried out similarly to the procedures used in the affinity enrichment process, except that after removing unbound phage, the wells were typically treated with rabbit anti-phage antibody, then with alkaline phosphatase (AP)-conjugated goat anti-rabbit antibody. The amount of alkaline phosphatase in each well was determined by standard methods. A similar ELISA procedure for use in the peptides on plasmids system is described in detail below.

By comparing test wells with control wells (no receptor), one can determine whether the fusion proteins bind to the receptor specifically. The phage pools found to bind to TPO-R were screened in a colony lift probing format using radiolabelled monovalent receptor. This probe can be produced using protein kinase A to phosphorylate a peptide sequence fused to the C-terminus of the soluble receptor. The “engineered” form of the TPO receptor is then expressed in host cells, typically CHO cells. Following PI-PLC harvest of the receptors, the receptor was tested for binding to TPO or TPO-R specific phage clones. The receptor is then labeled to high specific activity with 33 P for use as a monovalent probe to identify high affinity ligands using colony lifts.

Peptides found to bind specifically to the receptor were then synthesized as the free peptide (e.g., no phage) and tested in a blocking assay. The blocking assay was carried out in similar fashion to the ELISA, except that TPO or a reference peptide was added to the wells before the fusion protein (the control wells were of two types: (1) no receptor; and (2) no TPO or reference peptide). Fusion proteins for which the binding to the receptor was blocked by TPO or the reference peptide contain peptides in the random peptide portion that are preferred compounds of the invention.

TPO-R, as well as its extracellular domain, were produced in recombinant host cells. One useful form of TPO-R is constructed by expressing the protein as a soluble protein in baculovirus transformed host cells using standard methods; another useful form is constructed with a signal peptide for protein secretion and for glycophospholipid membrane anchor attachment. This form of anchor attachment is called “PIG-tailing”. See Caras, et al., Science, 243:1196-1198 (1989) and Lin, et al., Science, 249:677-679 (1990).

Using the PIG-tailing system, one can cleave the receptor from the surface of the cells expressing the receptor (e.g., transformed CHO cells selected for high level expression of receptor with a cell sorter) with phospholipase C. The cleaved receptor still comprises a carboxy terminal sequence of amino acids, called the “HPAP tail”, from the signal protein for membrane attachment and can be immobilized without further purification. The recombinant receptor protein can be immobilized by coating the wells of microtiter plates with an anti-HPAP tail antibody (Ab 179 or MAb 179), blocking non-specific binding with bovine serum albumin (BSA) in PBS, and then binding cleaved recombinant receptor to the antibody. Using this procedure, one should perform the immobilization reaction in varying concentrations of receptor to determine the optimum amount for a given preparation, because different preparations of recombinant protein often contain different amounts of the desired protein. In addition, one should ensure that the immobilizing antibody is completely blocked (with TPO or some other blocking compound) during the affinity enrichment process. Otherwise, unblocked antibody can bind undesired phage during the affinity enrichment procedure. One can use peptides that bind to the immobilizing antibody to block unbound sites that remain after receptor immobilization to avoid this problem or one can simply immobilize the receptor directly to the wells of microtiter plates, without the aid of an immobilizing antibody. See U.S. patent application Ser. No. 07/947,339, filed Sep. 18, 1992, incorporated herein by reference.

When using random peptide generation systems that allow for multivalent ligand-receptor interaction, one must recognize that the density of the immobilized receptor is an important factor in determining the affinity of the ligands that can bind to the immobilized receptor. At higher receptor densities (e.g., each anti-receptor antibody-coated well treated with 0.25 to 0.5 mg of receptor), multivalent binding is more likely to occur than at lower receptor densities (e.g., each anti-receptor antibody-coated well treated with 0.5 to 1 ng of the receptor). If multivalent binding is occurring, then one will be more likely to isolate ligands with relatively lower affinity, unless one uses high densities of immobilized receptor to identify lead compounds and uses lower receptor densities to isolate higher affinity derivative compounds.

To discriminate among higher affinity peptides, a monovalent receptor probe frequently is used. This probe can be produced using protein kinase A to phosphorylate a peptide sequence fused to the C-terminus of the soluble receptor. The “engineered” form of the TPO receptor is then expressed in host cells, typically CHO cells. Following PI-PLC harvest of the receptors, the receptor was tested for binding to TPO or TPO-R specific phage clones. The receptor is then labeled to high specific activity with 33 P for use as a monovalent probe to identify high affinity ligands using colony lifts.

Preferred screening methods to facilitate identification of peptides which bind TPO-R involve first identifying lead peptides which bind to the extracellular domain of the receptor and then making other peptides which resemble the lead peptides. Specifically, using a pIII or pVIII-based peptides on phage system, a random library can be screened to discover a phage that presents a peptide that binds to TPO-R. The phage DNAs are sequenced to determine the sequences of the peptides displayed on the surface of the phages.

Clones capable of specific binding to the TPO-R were identified from a random linear 10-mer pVIII library and a random cyclic 10-mer and 12-mer pVIII libraries. The sequences of these peptides serve as the basis for the construction of other peptide libraries designed to contain a high frequency of derivatives of the initially identified peptides. These libraries can be synthesized so as to favor the production of peptides that differ from the binding peptide in only a few residues. This approach involves the synthesis of an oligonucleotide with the binding peptide coding sequence, except that rather than using pure preparations of each of the four nucleoside triphosphates in the synthesis, one uses mixtures of the four nucleoside triphosphates (i.e., 55% of the “correct” nucleotide, and 15% each of the other three nucleotides is one preferred mixture for this purpose and 70% of the “correct” nucleotide and 10% of each of the other three nucleotides is another preferred mixture for this purpose) so as to generate derivatives of the binding peptide coding sequence.

A variety of strategies were used to derivatize the lead peptides by making “mutagenesis on a theme” libraries. These included a pVIII phagemid mutagenesis library based on the consensus sequence mutagenized at 70:10:10:10 frequency and extended on each terminus with random residues to produce clones which include the sequence XXXX (C, S, P, or R) TLREWL XXXXXX (C or S). A similar extended/mutagenized library was constructed using the peptides-on-plasmids system to produce clones which include the sequence XXXXX (C, S, P, or R) TLREWL XXXXXXX. An additional extended/mutagenized library, XXXX (C, S, P, or R) TLREWL XXXXXX (C or S), was constructed using the polysome display system. All three libraries were screened with peptide elution and probed with radiolabeled monovalent receptor.

The “peptides on plasmids” techniques was also used for peptide screening and mutagenesis studies and is described in greater detail in U.S. Pat. No. 5,338,665, which is incorporated herein by reference for all purposes. According to this approach, random peptides are fused at the C-terminus of LacI through expression from a plasmid vector carrying the fusion gene. Linkage of the LacI-peptide fusion to its encoding DNA occurs via the lacO sequences on the plasmid, forming a stable peptide-LacI-plasmid complex that can be screened by affinity purification (panning) on an immobilized receptor. The plasmids thus isolated can then be reintroduced into E. coli by electroporation to amplify the selected population for additional rounds of screening, or for the examination of individual clones.

In addition, random peptide screening and mutagenesis studies were performed using a modified C-terminal Lac-I display system in which display valency was reduced (“headpiece dimer” display system). The libraries were screened and the resulting DNA inserts were cloned as a pool into a maltose binding protein (MBP) vector allowing their expression as a C-terminal fusion protein. Crude cell lysates from randomly picked individual MBP fusion clones were then assayed for TPO-R binding in an ELISA format, as discussed above.

Peptide mutagenesis studies were also conducted using the polysome display system, as described in co-pending application U.S. patent application Ser. No. 08/300,262, filed Sep. 2, 1994, which is a continuation-in-part application based on U.S. patent application Ser. No. 08/144,775, filed Oct. 29, 1993 and PCT WO 95/11992, each of which is incorporated herein by references for all purposes. A mutagenesis library was constructed based on the sequence X X X X (C,P,R, or S) t l r e f l X X X X X X (C or S), in which X represents a random NNK codon, and the lower case letters represent amino acid codons containing 70:10:10:10 mutagenesis at positions 1 and 2 and K (G or T) at position 3 of the codon. The library was panned for 5 rounds against TPO receptor which had been immobilized on magnetic beads. After the fifth round, the PCR amplified pool was cloned into pAFF6 and the ELISA positive clones were sequenced. The sequences were subcloned into an MBP vector and their binding affinities were determined by an MBP ELISA.

To immobilize the TPO-R for polysome screening, Ab 179 was first chemically conjugated to tosyl-activated magnetic beads (available from Dynal Corporation) as described by the manufacturer. The beads were incubated with antibody in 0.5 M borate buffer (pH 9.5) overnight at room temperature. The beads were washed and combined with TPO-R containing the “HPAP” tail. The antibody coated beads and receptor were incubated for 1 hour at 40° C., and the beads were washed again prior to adding the polysome library.

Screening of the various libraries described above yielded the TPO receptor binding peptides shown in Tables 1 and 2 below, as well as others not listed herein. The peptides are set forth herein starting from the N-terminus to the C-terminus. Such peptides typically have NH 2 — as the amino terminal and —COOH as the acid terminal. Alternatively, however, such peptides can have NH 2 — as the amino terminal and —CONH 2 as the acid terminal, or X— as the amino terminal and —COOH as the acid terminal, wherein X is, for example, Ac, Z, Boc, etc.

TABLE 1
Peptide
R E G P T L R Q W M
R E G P T L R Q W M
S R G M T L R E W L
E G P T L R G W L A
R E G Q T L K E W L
E R G P F W A K A C
R E G P R C V M W M
C S G L T L R E W L V C
C L T G P F V T Q W L Y E C
C G E G L T L T Q W L E H C
C R A G P T L L E W L T L C
C R A G P T L L E W L T L C
C R Q G P T L T A W L L E C
C A D G P T L R E W I S F C
C E L V G P S L M S W L T C
C G T E G P T L S T W L D C
C D Q L G V T L S R W L EC
S G T G L T L R E W L G S F S L L S
C P E G P T L L Q W L K R G Y S S C
R G D G P T L SQ W L Y S L M I M C
M V A G P T L R E F I A S L P I H C
S M Q G P T F R E W V S M M K V L C
S V Q C G P T L R Q W L A A R N H L S
G N A D G P T L R Q W L E G R R P K N
S V R C G P T L R Q W L A A R T H L S
L A I E G P T L R Q W L H G N G R D T
H G R V G P T L R E W K T Q V A T K K
C A D G P T L R E W I S F C
I S D G P T L K E W L S V T R G A S
S I E G P T L R E W L T S R T P H S
T I K G P T L R Q W L K S R E H T S
G N A D G P T L R Q W L E G R R P K N
S I E G P T L R E W L T S R T P H S
I S D G P T L K E W L S V T R G A S

TABLE 2
Peptide
C S L E D L R K R C
C R R S E L L E R C
C T F K Q F L D G C
C T R G E W L R C C
C T L R Q W L Q G C
C T L E E L R A C C
C T R E E L M R L C
C Q R A D L I N F C
C N R N D L L L F C
C T R T E W L H G C
C T L E F M N G C
C S L G E L R R L C
C N I N Q L R S I C
C T M R Q F L V C C
C T R S E W L E R C
C T L H E Y L S G C
C T R E E L L R Q C
C T F R E F V N G C
C S R A D F L A A C
C S C A Q V V Q C C
C T L R Q W I L L G M C
C T L R E W L H G G F C
C T L R A W L M S E T C
C T L R A W L M E S C C
C T F Q V W K L A R N C
C L L R E W L D X R T C
C V L R E W L L X X S C
C L L S E F L A G Q Q C
C S L R Q Y L D F G L G S C
C T L Q E L K Q S S L Y E C
C D L S E L K T H G Y A Y C
C K L S D W L M N G V A A C
C S L Q E F L S H G G Y V C
C S L K E F L H S G L M Q C
C T F R Q L L E Y G V S S C
C T M R E F L V A S G V A C
C T L A E F L A S G V E Q C
C T L A E F L A S G V E Q C
C T L K E W L V S H E V W C
C T L R E F L S L G M N A C
C T L R E F L D P T T A V C
C S L L E F L A L G V A L C
G G G R G C T L K Q W K Q G D C G R S
C N R S Q L L A A C
C T L Q Q W L S G C
C T L R E F K A G C
C T R A Q F L K G C
C T L R E F N R G C
C T L S D F K R G C
C T F R Q W K E A C
C T L S E F R G G C
C T L Q E F L E G C
C T L Q Q W K D G C
C T R S Q W L E G C
C S L Q E F K H G C
C T L G E W K R G C
C T L W G C G K R G C
C T L Q E W R G G C
C T R L S G C W L C
C T R T Q W L L D C
C T L A E F R R G C
C T S T Q W L L A C
C S R S Q F L R S C
C T L R E W L E G C
C T L R E F L L M G A C
C T L K E W L L W S S C
C T L L E W L R N P V C
C T L R Q W L G D A W C
C T L G Q W L Q M G M C
C T L R E W V F A G L C
C L L L E F L S G A D C
C T L G E F L A G H L C
C R L R E F L V D L T C
C S F R S W L V D Q T C
C T L R E W L E D L G C
C T L Q D W L V S W T C
C T L S E W L S E L S C
C T L M Q W L G G W P C
C T L R E W L S Y G T C
C T L Q E W L S G G L C
G S H G C T L R E W L C M K I V P C
Q W Q G C T L R D C I L R G V F W S
S V N S C T L R E F L T G C R V F C
S Y D G C T L R H W L M D I Y G D C
Q R S G C T L R D W V L L N C L A S
N Y R G C T L S Q W V S E Q I V G C
G R S G C T L R E Y L G G M C Y L S
A S W Y C T V P E L M E M Q L P E C
G S T G C T L R E X L H M L G L D C
A C E G C T L R Q W L E Y V R V G C
A Q R G C T L Q Y F V S Y G X D M C
G V C G C T L R E F L A I P H T S C
S E G G C T L R E W V A S S L A N C
S N S R C T L R E W I I Q G C D F S
S N S R C T L R E W I I Q G C D F S
C L G C T L S Q W R K R T R C D T H
Y R G C S R A Q L L G G E C R K K
G R G C T L K Q W K Q G D C G R S
V R G G C A L R D W V A G E C F D W T
L W R G C T L N G F K S R H C G S P E
C T L R S W K H R G C A P
G R G C T R A Q W L A G C C T G H
R A G C T L R E F R K G C L A L
K R G C T L A E M I R G C N R S N
G R G C T L K Q W K Q G D C G R S
R W R G C S L A K L K K G A A C G R G
R G G C T L R E W R R V R V I N
G R G C T L K Q W K Q G D C G R S
R Y G C T R H Q W L V G T C V R H

IC 50 values for some additional representative peptides are given in the table below. A variety of methods can be used to evaluate IC 50 values. For example, an equilibrium binding ELISA assay, using either MBP-TPO or lacI-peptide tracer, was used to determine whether the peptides inhibit the binding of TPO to the extracellular domain of the TPO receptor. Typically, the IC 50 value were determined using the free peptide. The IC 50 value can be determined using the free peptide, which optionally can be C-terminally amidated, or can be prepared as an ester or other carboxy amide.

To recreate the exact sequence displayed on the phage, the N-terminal and C-terminal amino acids of the synthetic peptides are often preceded by one or two glycine residues. These glycines are not believed to be necessary for binding or activity. Likewise, to mimic the exact sequence of peptides displayed on polysomes, the C-terminal amino acids of the synthetic peptides are often preceded by the sequence M A S. Again, this sequence is not believed to be necessary for binding or activity.

IC 50 values are indicated symbolically by the symbols “−”, “+”, and “++”. For examples, those peptides which showed IC 50 values in excess of 200 μM are indicated with a “−”. Those peptides which gave IC 50 values of less than or equal to 200 μM are given a “+”, while those which gave IC 50 values of 500 nm or less are indicated with a “++”. Those peptides which gave IC 50 values at or near the cutoff point for a particular symbol are indicated with a hybrid designator, e.g., “+/−”, Those peptides for which IC 50 values were not determined are listed as “N.D.”. The IC 50 value for peptides having the structure: G G C T L R E W L H G G F C G G was 500 nm or less. (Note the N-terminal and C-terminal amino acids were preceded by two glycines to recreate the exact sequence displayed by the phage. These glycines are not believed to be necessary for binding or activity.)

TABLE 3
Peptide                          Affinity
G G C A D G P T L R E W I S F C G G ++
G N A D G P T L R Q W L E G R R P K N ++
G G C A D G P T L R E W I S F C G G K ++
T I K G P T L R Q W L K S R E H T S ++
G P T L R Q W L                  −
L A I E G P T L R Q W L H G N G R D T ++
S I E G P T L R E W L T S R T P H S ++

The tables above, especially Table 3, illustrate that a preferred core peptide comprises a sequence of amino acids:

X 1 X 2 X 3 X 4 X 5 X 6 X 7

where X 1 is C, L, M, P, Q, V; X 2 is F, K, L, N, Q, R, S, T or V; X 3 is C, F, I, L, M, R, S, V or W; X 4 is any of the 20 genetically coded L-amino acids; X 5 is A, D, E, G, K, M, Q, R, S, T, V or Y; X 6 is C, F, G, L, M, S, V, W or Y; and X 7 is C, G, I, K, L, M, N, R or V.

In a preferred embodiment the core peptide comprises a sequence of amino acids:

 X 8 G X 1 X 2 X 3 X 4 X 5 W X 7

where X 1 is L, M, P, Q, or V; X 2 is F, R, S, or T; X 3 is F, L, V, or W; X 4 is A, K, L, M, R, S, V, or T; X 5 is A, E, G, K, M, Q, R, S, or T; X 7 is C, I, K, L, M or V; and each X 8 residue is independently selected from any of the 20 genetically coded L-amino acids, their stereoisomeric D-amino acids; and non-natural amino acids. Preferably, each X 8 residue is independently selected from any of the 20 genetically coded L-amino acids and their stereoisomeric D-amino acids. In a preferred embodiment, X 1 is P; X 2 is T; X 3 is L; X 4 is R; X 5 is E or Q; and X 7 is I or L.

More preferably, the core peptide comprises a sequence of amino acids:

X 9 X 8 G X 1 X 2 X 3 X 4 X 5 W X 7

where X 9 is A, C, E, G, I, L, M, P, R, Q, S, T, or V; and X 8 is A, C, D, E, K, L, Q, R, S, T, or V. More preferably, X 9 is A or I; and X 8 is D, E, or K.

Particularly preferred peptides include: G G C A D G P T L R E W I S F C G G; G N A D G P T L R Q W L E G R R P K N; G G C A D G P T L R E W I S F C G G K; T I K G P T L R Q W L K S R E H T S; S I E G P T L R E W L T S R T P H S; L A I E G P T L R Q W L H G N G R D T; C A D G P T L R E W I S F C; and I E G P T L R Q W L A A R A.

In further embodiments of the invention, preferred peptides for use in this invention include peptides having a core structure comprising sequence of amino acids: sequence of amino acids:

C X 2 X 3 X 4 X 5 X6 X 7

where X 2 is F, K, L, N, Q, R, S, T or V; X 3 is C, F, I, L, M, R, S or V; X 4 is any of the 20 genetically coded L-amino acids; X 5 is A, D, E, G, S, V or Y; X 6 is C, F, G, L, M, S, V, W or Y; and X 7 is C, G, I, K, L, M, N, R or V. In a more preferred embodiment, X 4 is A, E, G, H, K, L, M, P, Q, R, S, T, or W. In a further embodiment, X 2 is S or T; X 3 is L or R; X 4 is R; X 5 is D, E, or G; X 6 is F, L, or W; and X 7 is I, K, L, R, or V. Particularly preferred peptides include: G G C T L R E W L H G G F C G G.

In a further embodiment, preferred peptides for use in this invention include peptides having a structure comprising a sequence of amino acids:

X 8 C X 2 X 3 X 4 X 5 X 6 X 7

where X 2 is F, K, L, N, Q, R, S, T or V; X 3 is C, F, I, L, M, R, S, V or W; X 4 is any of the 20 genetically coded L-amino acids; X 5 is A, D, E, G, K, M, Q, R, S, T, V or Y; X 6 is C, F, G, L, M, S, V, W or Y; X 7 is C, G, I, K, L, M, N, R or V; and X 8 is any of the 20 genetically coded L-amino acids. In some embodiments, X 8 is preferably G, S, Y, or R.

Peptides and peptidomimetics having an IC 50 of greater than about 100 mM lack sufficient binding to permit use in either the diagnostic or therapeutic aspects of this invention. Preferably, for diagnostic purposes, the peptides and peptidomimetics have an IC 50 of about 2 mM or less and, for pharmaceutical purposes, the peptides and peptidomimetics have an IC 50 of about 100 μM or less.

The binding peptide sequence also provides a means to determine the minimum size of a TPOR binding compound of the invention. Using the “encoded synthetic library” (ESL) system or the “very large scale immobilized polymer synthesis” system, one can not only determine the minimum size of a peptide with such activity, but one can also make all of the peptides that form the group of peptides that differ from the preferred motif (or the minimum size of that motif) in one, two, or more residues. This collection of peptides can then be screened for ability to bind to TPO-receptor. These immobilized polymers synthesis systems or other peptide synthesis methods can also be used to synthesize truncation analogs, deletion analogs, substitution analogs, and combinations thereof all of the peptide compounds of the invention.

The peptides and peptide mimetics of the present invention were also evaluated in a thrombopoietin dependent cell proliferation assay, as described in greater detail in Example 2 below. Cell proliferation is measured by techniques known in the art, such as an MIT assay which correlates with 3 H-thymidine incorporation as an indication of cell proliferation (see Mossmann, J. Immunol. Methods, 65:55 (1983)). The peptides tested stimulated proliferation of TPO-R transfected Ba/F3 cells in a dose dependent manner as shown in FIG. 1 A. These peptides have no effect on the parental cell line as shown in FIG. 1 B.

FIGS. 7 to 9 show the results of a further assay evaluating activity of the peptides and peptide mimetics of the invention. In this assay mice are made thrombocytopenic with carboplatin. FIG. 7 depicts typical results when Balb/C mice are treated with carboplatin (125 mg/kg intraperitoneally) on Day 0. The dashed lines represent untreated animals from three experiments. The solid line represent carboplatin-treated groups in three experiments. The heavy solid lines represent historical data. FIG. 8 depicts the effect of carboplatin titration on platelet counts in mice treated with the indicated amounts of carboplatin (in mg/kg, intraperitoneally (ip) on Day 0). FIG. 9 depicts amelioration of carboplatin-induced thrombocytopenia on Day 10 by peptide AF12513 (513). Carboplatin (CBP; 50-125 mg/kg, intraperitoneally) was administered on Day 0. AF12513 (1 mg/kg, ip) was given on Days 1-9. These results show the peptides of the invention can ameliorate thrombocytopenia in a mouse model.

In addition, certain peptides of the present invention can be dimerized or oligomerized, thereby increasing the affinity and/or activity of the compounds. To investigate the effect that peptide dimerization/oligomerization has on TPO mimetic potency in cell proliferation assays, a C-terminally biotinylated analog of the peptide G G C A D G P T L R E W I S F C G G was synthesized (G G C A D G P T L R E W I S F C G G K (Biotin)). The peptide was preincubated with streptavidin in serum-free HEPES-buffered RPMI at a 4:1 molar ratio. The complex was tested for stimulation of cell proliferation of TPO-R transfected Ba/F3 cells, as above, alongside free biotinylated peptide and the unbiotinylated parental peptide. FIG. 2A shows the results of the assay for the complexed biotinylated peptide (AF 12885 with streptavidin (SA)) for both the transfected and parental cell lines. FIG. 2B shows the results of the assay for the free biotinylated peptide (AF 12285) for both the transfected and parental cell lines. FIG. 2C shows the results of the assay for streptavidin alone for both the transfected and parental cell lines. These figures illustrate that the pre-formed complex was approximately 10 times more potent as the free peptide.

The specificity of the binding and activity of the peptides of the invention was also examined by studying the cross reactivity of the peptides for the erythropoietin receptor (EPO-R). The EPO-R is also a member of the hematopoietin growth factor receptor family, as is TPO-R. The peptides of the invention, as well as TPO, EPO, and a known EPO-binding peptide, were examined in a cell proliferation assay using an EPO-dependent cell line. This assay utilized FDCP-1, a growth factor dependent murine multi-potential primitive hematopoietic progenitor cell line (see, e.g., Dexter, et al., J. Exp. Med., 152:1036-1047 (1981)) as the parental cell line. This cell line can proliferate, but not differentiate when supplemented with WEHI-3-conditioned media (a medium that contains IL-3, ATCC number T1B68). The parental cell line is transfected with human or murine EPO-R to produce the FDCP-1-EPO-R cell line. These transfected cell lines can proliferate, but not differentiate in the presence of human or murine EPO.

The cells were grown to half stationary density in the presence of the necessary growth factors. The cells are then washed in PBS and starved for 16-24 hours in whole media without the growth factors. After determining the viability of the cells, stock solutions (in whole media without the growth factors) are made to give about 10 5 cells per 50 microliters. Serial dilutions of the compounds (typically, the free solution phase peptide as opposed to a phage-bound or other bound or immobilized peptide) to be tested are made in 96-well tissue culture plates for a final volume of 50 microliters per well. Cells (50 microliters) are added to each well and the cells are incubated for 24-48 hours, at which point the negative controls should die or be quiescent. Cell proliferation is then measured by techniques known in the art, such as an MTT assay.

FIGS. 3A-G show the results of a series of control experiments showing the activity of TPO, the peptides of the present invention, EPO, and EPO-R binding peptides in a cell proliferation assay using either the TPO-R transfected Ba/F3 cell line and its corresponding parental line, or an EPO-dependent cell line and its corresponding parental line. FIG. 3A depicts the results for TPO in the cell proliferation assay using the TPO-R transfected Ba/F3 cell line and its corresponding parental line. FIG. 3B depicts the results for EPO in the cell proliferation assay using the TPO-R transfected Ba/F3 cell line and its corresponding parental line. FIG. 3C depicts the results for complexed biotinylated peptide (AF 12285 with streptavidin (SA)) and a complexed form of a biotinylated EPO-R binding peptide (AF 11505 with SA) in the TPO-R transfected Ba/F3 cell line. The results for the corresponding parental cell line are shown in FIG. 3 D. FIG. 3E depicts the results for TPO in the cell proliferation assay using the EPO-dependent cell line. FIG. 3F depicts the results for EPO in the cell proliferation assay using the EPO-dependent cell line. FIG. 3G depicts the results for complexed biotinylated peptide (AF 12285 with streptavidin (SA)) and the complexed form of a biotinylated EPO-R binding peptide (AF 11505 with SA) in the EPO-dependent cell line. These results show that the peptides of the invention bind and activate the TPO-R with a high degree of specificity.

IV. Preparation of Peptides and Peptide Mimetics

A. Solid Phase Synthesis

The peptides of the invention can be prepared by classical methods known in the art, for example, by using standard solid phase techniques. The standard methods include exclusive solid phase synthesis, partial solid phase synthesis methods, fragment condensation, classical solution synthesis, and even by recombinant DNA technology. See, e.g., Merrifield, J. Am. Chem. Soc., 85:2149 (1963), incorporated herein by reference. On solid phase, the synthesis is typically commenced from the C-terminal end of the peptide using an alpha-amino protected resin. A suitable starting material can be prepared, for instance, by attaching the required alpha-amino acid to a chloromethylated resin, a hydroxymethyl resin, or a benzhydrylamine resin. One such chloromethylated resin is sold under the tradename BIO-BEADS SX-1 by Bio Rad Laboratories, Richmond, Calif., and the preparation of the hydroxymethyl resin is described by Bodonszky, et al., Chem. Ind. (London), 38:1597 (1966). The benzhydrylamine (BHA) resin has been described by Pietta and Marshall, Chem. Commn., 650 (1970) and is commercially available from Beckman Instruments, Inc., Palo Alto, Calif., in the hydrochloride form.

Thus, the compounds of the invention can be prepared by coupling an alpha-amino protected amino acid to the chloromethylated resin with the aid of, for example, cesium bicarbonate catalyst, according to the method described by Gisin, Helv. Chim. Acta., 56:1467 (1973). After the initial coupling, the alpha-amino protecting group is removed by a choice of reagents including trifluoroacetic acid (TFA) or hydrochloric acid (HCl) solutions in organic solvents at room temperature.

The alpha-amino protecting groups are those known to be useful in the art of stepwise synthesis of peptides. Included are acyl type protecting groups (e.g., formyl, trifluoroacetyl, acetyl), aromatic urethane type protecting groups (e.g. benzyloxycarboyl (Cbz) and substituted Cbz), aliphatic urethane protecting groups (e.g., t-butyloxycarbonyl (Boc), isopropyloxycarbonyl, cyclohexyloxycarbonyl) and alkyl type protecting groups (e.g., benzyl, triphenylmethyl). Boc and Fmoc are preferred protecting groups. The side chain protecting group remains intact during coupling and is not split off during the deprotection of the amino-terminus protecting group or during coupling. The side chain protecting group must be removable upon the completion of the synthesis of the final peptide and under reaction conditions that will not alter the target peptide.

The side chain protecting groups for Tyr include tetrahydropyranyl, tert-butyl, trityl, benzyl, Cbz, Z—Br—Cbz, and 2,5-dichlorobenzyl. The side chain protecting groups for Asp include benzyl, 2,6-dichlorobenzyl, methyl, ethyl, and cyclohexyl. The side chain protecting groups for Thr and Ser include acetyl, benzoyl, trityl, tetrahydropyranyl, benzyl, 2,6-dichlorobenzyl, and Cbz. The side chain protecting group for Thr and Ser is benzyl. The side chain protecting groups for Arg include nitro, Tosyl (Tos), Cbz, adamantyloxycarbonyl mesitoylsulfonyl (Mts), or Boc. The side chain protecting groups for Lys include Cbz, 2-chlorobenzyloxycarbonyl (2Cl-Cbz), 2-bromobenzyloxycarbonyl (2-BrCbz), Tos, or Boc.

After removal of the alpha-amino protecting group, the remaining protected amino acids are coupled stepwise in the desired order. An excess of each protected amino acid is generally used with an appropriate carboxyl group activator such as dicyclohexylcarbodiimide (DCC) in solution, for example, in methylene chloride (CH 2 Cl 2 ), dimethyl formamide (DMF) mixtures.

After the desired amino acid sequence has been completed, the desired peptide is decoupled from the resin support by treatment with a reagent such as trifluoroacetic acid or hydrogen fluoride (HF), which not only cleaves the peptide from the resin, but also cleaves all remaining side chain protecting groups. When the chloromethylated resin is used, hydrogen fluoride treatment results in the formation of the free peptide acids. When the benzhydrylamine resin is used, hydrogen fluoride treatment results directly in the free peptide amide. Alternatively, when the chloromethylated resin is employed, the side chain protected peptide can be decoupled by treatment of the peptide resin with ammonia to give the desired side chain protected amide or with an alkylamine to give a side chain protected alkylamide or dialkylamide. Side chain protection is then removed in the usual fashion by treatment with hydrogen fluoride to give the free amides, alkylamides, or dialkylamides.

These solid phase peptide synthesis procedures are well known in the art and further described by John Morrow Stewart and Janis Dillaha Young, Solid Phase Peptide Syntheses (2nd Ed., Pierce Chemical Company, 1984).

Using the “encoded synthetic library” or “very large scale immobilized polymer synthesis” system described in U.S. patent application Ser. No. 07/492,462, filed Mar. 7, 1990; Ser. No. 07/624,120, filed Dec. 6, 1990; and Ser. No. 07/805,727, filed Dec. 6, 1991; one can not only determine the minimum size of a peptide with such activity, one can also make all of the peptides that form the group of peptides that differ from the preferred motif (or the minimum size of that motif) in one, two, or more residues. This collection of peptides can then be screened for ability to bind to TPO-R. This immobilized polymer synthesis system or other peptide synthesis methods can also be used to synthesize truncation analogs and deletion analogs and combinations of truncation and deletion analogs of all of the peptide compounds of the invention.

B. Synthetic Amino Acids

These procedures can also be used to synthesize peptides in which amino acids other than the 20 naturally occurring, genetically encoded amino acids are substituted at one, two, or more positions of any of the compounds of the invention. For instance, naphthylalanine can be substituted for tryptophan, facilitating synthesis. Other synthetic amino acids that can be substituted into the peptides of the present invention include L-hydroxypropyl, L-3, 4-dihydroxy-phenylalanyl, d amino acids such as L-d-hydroxylysyl and D-d-methylalanyl, L-α-methylalanyl, β amino acids, and isoquinolyl. D amino acids and non-naturally occurring synthetic amino acids can also be incorporated into the peptides of the present invention (see, e.g., Roberts, et al., Unusual Amino/Acids in Peptide Synthesis, 5(6):341-449 (1983)).

One can replace the naturally occurring side chains of the 20 genetically encoded amino acids (or D amino acids) with other side chains, for instance with groups such as alkyl, lower alkyl, cyclic 4-, 5-, 6-, to 7-membered alkyl, amide, amide lower alkyl, amide di(lower alkyl), lower alkoxy, hydroxy, carboxy and the lower ester derivatives thereof, and with 4-, 5-, 6-, to 7-membered hetereocyclic. In particular, proline analogs in which the ring size of the proline residue is changed from 5 members to 4, 6, or 7 members can be employed. Cyclic groups can be saturated or unsaturated, and if unsaturated, can be aromatic or non-aromatic. Heterocyclic groups preferably contain one or more nitrogen, oxygen, and/or sulphur heteroatoms. Examples of such groups include the furazanyl, furyl, imidazolidinyl, imidazolyl, imidazolinyl, isothiazolyl, isoxazolyl, morpholinyl (e.g. morpholino), oxazolyl, piperazinyl (e.g. 1-piperazinyl), piperidyl (e.g. 1-piperidyl, piperidino), pyranyl, pyrazinyl, pyrazolidinyl, pyrazolinyl, pyrazolyl, pyridazinyl, pyridyl, pyrimidinyl, pyrrolidinyl (e.g. 1-pyrrolidinyl), pyrrolinyl, pyrrolyl, thiadiazolyl, thiazolyl, thienyl, thiomorpholinyl (e.g. thiomorpholino), and triazolyl. These heterocyclic groups can be substituted or unsubstituted. Where a group is substituted, the substituent can be alkyl, alkoxy, halogen, oxygen, or substituted or unsubstituted phenyl.

One can also readily modify the peptides of the instant invention by phosphorylation (see, e.g., W. Bannwarth, et al., Biorganic and Medicinal Chemistry Letters, 6(17):2141-2146 (1996)), and other methods for making peptide derivatives of the compounds of the present invention are described in Hruby, et al., Biochem. J., 268(2):249-262 (1990). Thus, the peptide compounds of the invention also serve as a basis to prepare peptide mimetics with similar biological activity.

C. Terminal Modifications

Those of skill in the art recognize that a variety of techniques are available for constructing peptide mimetics with the same or similar desired biological activity as the corresponding peptide compound but with more favorable activity than the peptide with respect to solubility, stability, and susceptibility to hydrolysis and proteolysis. See, for example, Morgan, et al., Ann. Rep. Med. Chem., 24:243-252 (1989). The following describes methods for preparing peptide mimetics modified at the N-terminal amino group, the C-terminal carboxyl group, and/or changing one or more of the amido linkages in the peptide to a non-amido linkage. It being understood that two or more such modifications can be coupled in one peptide mimetic structure (e.g., modification at the C-terminal carboxyl group and inclusion of a —CH 2 -carbamate linkage between two amino acids in the peptide).

1. N-TERMINAL MODIFICATIONS

The peptides typically are synthesized as the free acid but, as noted above, could be readily prepared as the amide or ester. One can also modify the amino and/or carboxy terminus of the peptide compounds of the invention to produce other compounds of the invention. Amino terminus modifications include methylation (i.e., —NHCH 3 or —NH(CH 3 ) 2 ), acetylation, adding a benzyloxycarbonyl group, or blocking the amino terminus with any blocking group containing a carboxylate functionality defined by RCOO—, where R is selected from the group consisting of naphthyl, acridinyl, steroidyl, and similar groups. Carboxy terminus modifications include replacing the free acid with a carboxamide group or forming a cyclic lactam at the carboxy terminus to introduce structural constraints.

Amino terminus modifications are as recited above and include alkylating, acetylating, adding a carbobenzoyl group, forming a succinimide group, etc. (See, e.g., Murray, et al., Burger's Medicinal Chemistry and Drug Discovery, 5th ed., Vol. 1, Manfred E. Wolf, ed., John Wiley and Sons, Inc. (1995).) Specifically, the N-terminal amino group can then be reacted as follows:

(a) to form an amide group of the formula RC(O)NH—where R is as defined above by reaction with an acid halide [e.g., RC(O)Cl] or symmetric anhydride. Typically, the reaction can be conducted by contacting about equimolar or excess amounts (e.g., about 5 equivalents) of an acid halide to the peptide in an inert diluent (e.g., dichloromethane) preferably containing an excess (e.g., about 10 equivalents) of a tertiary amine, such as diisopropylethylamine, to scavenge the acid generated during reaction. Reaction conditions are otherwise conventional (e.g., room temperature for 30 minutes). Alkylation of the terminal amino to provide for a lower alkyl N-substitution followed by reaction with an acid halide as described above will provide for N-alkyl amide group of the formula RC(O)NR—;

(b) to form a succinimide group by reaction with succinic anhydride. As before, an approximately equimolar amount or an excess of succinic anhydride (e.g., about 5 equivalents) can be employed and the amino group is converted to the succinimide by methods well known in the art including the use of an excess (e.g., ten equivalents) of a tertiary amine such as diisopropylethylamine in a suitable inert solvent (e.g., dichloromethane). See, for example, Wollenberg, et al., U.S. Pat. No. 4,612,132 which is incorporated herein by reference in its entirety. It is understood that the succinic group can be substituted with, for example, C 2 -C 6 alkyl or —SR substituents which are prepared in a conventional manner to provide for substituted succinimide at the N-terminus of the peptide. Such alkyl substituents are prepared by reaction of a lower olefin (C 2 -C 6 ) with maleic anhydride in the manner described by Wollenberg, et al., supra and —SR substituents are prepared by reaction of RSH with maleic anhydride where R is as defined above;

(c) to form a benzyloxycarbonyl-NH— or a substituted benzyloxycarbonyl-NH— group by reaction with approximately an equivalent amount or an excess of CBZ—Cl (i.e., benzyloxycarbonyl chloride) or a substituted CBZ—Cl in a suitable inert diluent (e.g., dichloromethane) preferably containing a tertiary amine to scavenge the acid generated during the reaction;

(d) to form a sulfonamide group by reaction with an equivalent amount or an excess (e.g., 5 equivalents) of R—S(O) 2 Cl in a suitable inert diluent (dichloromethane) to convert the terminal amine into a sulfonamide where R is as defined above. Preferably, the inert diluent contains excess tertiary amine (e.g., ten equivalents) such as diisopropylethylamine, to scavenge the acid generated during reaction. Reaction conditions are otherwise conventional (e.g., room temperature for 30 minutes);

(e) to form a carbamate group by reaction with an equivalent amount or an excess (e.g., 5 equivalents) of R—OC(O)Cl or R—OC(O)OC 6 H 4 -p-NO 2 in a suitable inert diluent (e.g., dichloromethane) to convert the terminal amine into a carbamate where R is as defined above. Preferably, the inert diluent contains an excess (e.g., about 10 equivalents) of a tertiary amine, such as diisopropylethylamine, to scavenge any acid generated during reaction. Reaction conditions are otherwise conventional (e.g., room temperature for 30 minutes); and

(f) to form a urea group by reaction with an equivalent amount or an excess (e.g., 5 equivalents) of R—N═C═O in a suitable inert diluent (e.g., dichloromethane) to convert the terminal amine into a urea (i.e., RNHC(O)NH—) group where R is as defined above. Preferably, the inert diluent contains an excess (e.g., about 10 equivalents) of a tertiary amine, such as diisopropylethylamine. Reaction conditions are otherwise conventional (e.g., room temperature for about 30 minutes).

2. C-TERMINAL MODIFICATIONS

In preparing peptide mimetics wherein the C-terminal carboxyl group is replaced by an ester (i.e., —C(O)OR where R is as defined above), the resins used to prepare the peptide acids are employed, and the side chain protected peptide is cleaved with base and the appropriate alcohol, e.g., methanol. Side chain protecting groups are then removed in the usual fashion by treatment with hydrogen fluoride to obtain the desired ester.

In preparing peptide mimetics wherein the C-terminal carboxyl group is replaced by the amide —C(O)NR 3 R 4 , a benzhydrylamine resin is used as the solid support for peptide synthesis. Upon completion of the synthesis, hydrogen fluoride treatment to release the peptide from the support results directly in the free peptide amide (i.e., the C-terminus is —C(O)NH 2 ). Alternatively, use of the chloromethylated resin during peptide synthesis coupled with reaction with ammonia to cleave the side chain protected peptide from the support yields the free peptide amide and reaction with an alkylamine or a dialkylamine yields a side chain protected alkylamide or dialkylamide (i.e., the C-terminus is —C(O)NRR 1 where R and R 1 are as defined above). Side chain protection is then removed in the usual fashion by treatment with hydrogen fluoride to give the free amides, alkylamides, or dialkylamides.

In another alternative embodiment, the C-terminal carboxyl group or a C-terminal ester can be induced to cyclize by internal displacement of the —OH or the ester (—OR) of the carboxyl group or ester respectively with the N-terminal amino group to form a cyclic peptide. For example, after synthesis and cleavage to give the peptide acid, the free acid is converted to an activated ester by an appropriate carboxyl group activator such as dicyclohexylcarbodiimide (DCC) in solution, for example, in methylene chloride (CH 2 Cl 2 ), dimethyl formamide (DMF) mixtures. The cyclic peptide is then formed by internal displacement of the activated ester with the N-terminal amine. Internal cyclization as opposed to polymerization can be enhanced by use of very dilute solutions. Such methods are well known in the art.

One can also cyclize the peptides of the invention, or incorporate a desamino or descarboxy residue at the terminii of the peptide, so that there is no terminal amino or carboxyl group, to decrease susceptibility to proteases or to restrict the conformation of the peptide. C-terminal functional groups of the compounds of the present invention include amide, amide lower alkyl, amide di(lower alkyl), lower alkoxy, hydroxy, and carboxy, and the lower ester derivatives thereof, and the pharmaceutically acceptable salts thereof.

In addition to the foregoing N-terminal and C-terminal modifications, the peptide compounds of the invention, including peptidomimetics, can advantageously be modified with or covalently coupled to one or more of a variety of hydrophilic polymers. It has been found that when the peptide compounds are derivatized with a hydrophilic polymer, their solubility and circulation half-lives are increased and their immunogenicity is masked. Quite surprisingly, the foregoing can be accomplished with little, if any, diminishment in their binding activity. Nonproteinaceous polymers suitable for use in accordance with the present invention include, but are not limited to, polyalkylethers as exemplified by polyethylene glycol and polypropylene glycol, polylactic acid, polyglycolic acid, polyoxyalkenes, polyvinylalcohol, polyvinylpyrrolidone, cellulose and cellulose derivatives, dextran and dextran derivatives, etc. Generally, such hydrophilic polymers have an average molecular weight ranging from about 500 to about 100,000 daltons, more preferably from about 2,000 to about 40,000 daltons and, even more preferably, from about 5,000 to about 20,000 daltons. In preferred embodiments, such hydrophilic polymers have an average molecular weights of about 5,000 daltons, 10,000 daltons and 20,000 daltons.

The peptide compounds of the invention can be derivatized with or coupled to such polymers using any of the methods set forth in Zallipsky, S., Bioconjugate Chem., 6:150-165 (1995); Monfardini, C, et al., Bioconjugate Chem., 6:62-69 (1995); U.S. Pat. Nos. 4,640,835; 4,496,689; 4,301,144; 4,670,417; 4,791,192; 4,179,337 or WO 95/34326, all of which are incorporated by reference in their entirety herein.

In a presently preferred embodiment, the peptide compounds of the present invention are derivatized with polyethylene glycol (PEG). PEG is a linear, water-soluble polymer of ethylene oxide repeating units with two terminal hydroxyl groups. PEGs are classified by their molecular weights which typically range from about 500 daltons to about 40,000 daltons. In a presently preferred embodiment, the PEGs employed have molecular weights ranging from 5,000 daltons to about 20,000 daltons. PEGs coupled to the peptide compounds of the present invention can be either branched or unbranched. (See, e.g., Monfardini, C., et al., Bioconjugate Chem., 6:62-69 (1995)). PEGs are commercially available from Shearwater Polymers, Inc. (Huntsville, Ala.), Sigma Chemical Co. and other companies. Such PEGs include, but are not limited to, monomethoxypolyethylene glycol (MePEG-OH), monomethoxypolyethylene glycol-succinate (MePEG-S), monomethoxypolyethylene glycol-succinimidyl succinate (MePEG-S-NHS), monomethoxypolyethylene glycol-amine (MePEG-NH 2 ), monomethoxypolyethylene glycol-tresylate (MePEG-TRES), and monomethoxypolyethylene glycol-imidazolyl-carbonyl (MePEG-IM).

Briefly, in one exemplar embodiment, the hydrophilic polymer which is employed, e.g., PEG, is preferably capped at one end by an unreactive group such as a methoxy or ethoxy group. Thereafter, the polymer is activated at the other end by reaction with a suitable activating agent, such as cyanuric halides (e.g., cyanuric chloride, bromide or fluoride), diimadozle, an anhydride reagent (e.g., a dihalosuccinic anhydride, such as dibromosuccinic anhydride), acyl azide, p-diazoiumbenzyl ether, 3-(p-diazoniumphenoxy)-2-hydroxypropylether) and the like. The activated polymer is then reacted with a peptide compound of the present invention to produce a peptide compound derivatized with a polymer. Alternatively, a functional group in the peptide compounds of the invention can be activated for reaction with the polymer, or the two groups can be joined in a concerted coupling reaction using known coupling methods. FIGS. 15-17 illustrate exemplar reactions schemes for derivatizing the peptide compounds of the invention with, for example, polyethylene glycol (PEG). It will be readily appreciated that the peptide compounds of the invention can be derivatized with PEG using a myriad of other reaction schemes known to and used by those of skill in the art.

In addition to derivatizing the peptide compounds of this invention with a hydrophilic polymer (e.g., PEG), it has now been discovered that other small peptides, e.g., other peptides or ligands that bind to a receptor, can also be derivatized with such hydrophilic polymers with little, if any, loss in biological activity (e.g., binding activity, agonist activity, antagonist activity, etc.). It has been found that when these small peptides are derivatized with a hydrophlilic polymer, their solubility and circulation half-lives are increased and their immunogenicity is decreased. Again, quite surprisingly, the foregoing can be accomplished with little, if any, loss in biological activity. In fat, in preferred embodiments, the derivatized peptides have an activity that is 0.1 to 0.01-fold that of the unmodified peptides. In more preferred embodiments, the derivatized peptides have an activity that is 0.1 to 1-fold that of the unmodified peptides. In even more preferred embodiments, the derivatized peptides have an activity that is greater than the unmodified peptides.

Peptides suitable for use in this embodiment generally include those peptides, i.e., ligands, that bind to a receptor, such as the TPO, EPO, IL-1, G-CSF and IL-5 receptors; the hematopoietic growth factor receptors; the cytokine receptors; the G-protein-linked receptors; the cell surface receptors, etc. Such peptides typically comprise about 150 amino acid residues or less and, more preferably, about 100 amino acid residues or less (e.g., ˜10-12 kDa). Hydrophilic polymers suitable for use in the present invention include, but are not limited to, polyalkylethers as exemplified by polyethylene glycol and polypropylene glycol, polylactic acid, polyglycolic acid, polyoxyalkenes, polyvinylalcohol, polyvinylpyrrolidone, cellulose and cellulose derivatives, dextran and dextran derivatives, etc. Generally, such hydrophilic polymers have an average molecular weight ranging from about 500 to about 100,000 daltons, more preferably from about 2,000 to about 40,000 daltons and, even more preferably, from about 5,000 to about 20,000 daltons. In preferred embodiments, such hydrophilic polymers have an average molecular weights of about 5,000 daltons, 10,000 daltons and 20,000 daltons. The peptide compounds of this invention can be derivatized with using the methods described above and in the cited references.

D. Backbone Modifications

Other methods for making peptide derivatives of the compounds of the present invention are described in Hruby, et al., Biochem J., 268(2):249-262 (1990), incorporated herein by reference. Thus, the peptide compounds of the invention also serve as structural models for non-peptidic compounds with similar biological activity. Those of skill in the art recognize that a variety of techniques are available for constructing compounds with the same or similar desired biological activity as the lead peptide compound but with more favorable activity than the lead with respect to solubility, stability, and susceptibility to hydrolysis and proteolysis. See Morgan, et al., Ann. Rep. Med. Chem., 24:243-252 (1989), incorporated herein by reference. These techniques include replacing the peptide backbone with a backbone composed of phosphonates, amidates, carbamates, sulfonamides, secondary amines, and N-methylamino acids.

Peptide mimetics wherein one or more of the peptidyl linkages [—C(O)NH—] have been replaced by such linkages as a —CH 2 -carbamate linkage, a phosphonate linkage, a —CH 2 -sulfonamide linkage, a urea linkage, a secondary amine (—CH 2 NH—) linkage, and an alkylated peptidyl linkage [—C(O)NR 6 — where R 6 is lower alkyl] are prepared during conventional peptide synthesis by merely substituting a suitably protected amino acid analogue for the amino acid reagent at the appropriate point during synthesis.

Suitable reagents include, for example, amino acid analogues wherein the carboxyl group of the amino acid has been replaced with a moiety suitable for forming one of the above linkages. For example, if one desires to replace a —C(O)NR— linkage in the peptide with a —CH 2 -carbamate linkage (—CH 2 OC(O)NR—), then the carboxyl (—COOH) group of a suitably protected amino acid is first reduced to the —CH 2 OH group which is then converted by conventional methods to a —OC(O)Cl functionality or a para-nitrocarbonate —OC(O)O—C 6 H 4 —p—NO 2 functionality. Reaction of either of such functional groups with the free amine or an alkylated amine on the N-terminus of the partially fabricated peptide found on the solid support leads to the formation of a —CH 2 OC(O)NR— linkage. For a more detailed description of the formation of such —CH 2 -carbamate linkages, see Cho, et al., Science, 261:1303-1305 (1993).

Similarly, replacement of an amido linkage in the peptide with a phosphonate linkage can be achieved in the manner set forth in U.S. patent application Ser. Nos. 07/943,805, 08/081,577, and 08/119,700, the disclosures of which are incorporated herein by reference in their entirety.

Replacement of an amido linkage in the peptide with a —CH 2 -sulfonamide linkage can be achieved by reducing the carboxyl (—COOH) group of a suitably protected amino acid to the —CH 2 OH group and the hydroxyl group is then converted to a suitable leaving group such as a tosyl group by conventional methods. Reaction of the tosylated derivative with, for example, thioacetic acid followed by hydrolysis and oxidative chlorination will provide for the —CH 2 —S(O) 2 Cl functional group which replaces the carboxyl group of the otherwise suitably protected amino acid. Use of this suitably protected amino acid analogue in peptide synthesis provides for inclusion of an —CH 2 S(O) 2 NR— linkage which replaces the amido linkage in the peptide thereby providing a peptide mimetic. For a more complete description on the conversion of the carboxyl group of the amino acid to a —CH 2 S(O) 2 Cl group, see, for example, Weinstein, B., Chemistry & Biochemistry of Amino Acids, Peptides and Proteins, Vol. 7, pp. 267-357, Marcel Dekker, Inc., New York (1983) which is incorporated herein by reference.

Replacement of an amido linkage in the peptide with a urea linkage can be achieved in the manner set forth in U.S. patent application Ser. No. 08/147,805 which application is incorporated herein by reference in its entirety.

Secondary amine linkages wherein a —CH 2 NH— linkage replaces the amido linkage in the peptide can be prepared by employing, for example, a suitably protected dipeptide analogue wherein the carbonyl bond of the amido linkage has been reduced to a CH 2 group by conventional methods. For example, in the case of diglycine, reduction of the amide to the amine will yield after deprotection H 2 NCH 2 CH 2 NHCH 2 COOH which is then used in N-protected form in the next coupling reaction. The preparation of such analogues by reduction of the carbonyl group of the amido linkage in the dipeptide is well known in the art (see, e.g., Michael W. Remington, Meth. in Mol. Bio., 35:241-247 (1994)).

The suitably protected amino acid analogue is employed in the conventional peptide synthesis in the same manner as would the corresponding amino acid. For example, typically about 3 equivalents of the protected amino acid analogue are employed in this reaction. An inert organic diluent such as methylene chloride or DMF is employed and, when an acid is generated as a reaction by-product, the reaction solvent will typically contain an excess amount of a tertiary amine to scavenge the acid generated during the reaction. One particularly preferred tertiary amine is diisopropylethylamine which is typically employed in about 10 fold excess. The reaction results in incorporation into the peptide mimetic of an amino acid analogue having a non-peptidyl linkage. Such substitution can be repeated as desired such that from zero to all of the amido bonds in the peptide have been replaced by non-amido bonds.

One can also cyclize the peptides of the invention, or incorporate a desamino or descarboxy residue at the terminii of the peptide, so that there is no terminal amino or carboxyl group, to decrease susceptibility to proteases or to restrict the conformation of the peptide. C-terminal functional groups of the compounds of the present invention include amide, amide lower alkyl, amide di(lower alkyl), lower alkoxy, hydroxy, and carboxy, and the lower ester derivatives thereof, and the pharmaceutically acceptable salts thereof. Examples of cyclized compounds are provided in Tables 4, 5, 6, 8, and 9.

E. Disulfide Bond Formation

The compounds of the present invention may exist in a cyclized form with an intramolecular disulfide bond between the thiol groups of the cysteines. Alternatively, an intermolecular disulfide bond between the thiol groups of the cysteines can be produced to yield a dimeric (or higher oligomeric) compound. One or more of the cysteine residues may also be substituted with a homocysteine. These intramolecular or intermolecular disulfide derivatives can be represented schematically as shown below:

wherein m and n are independently 1 or 2.

Other embodiments of this invention provide for analogs of these disulfide derivatives in which one of the sulfurs has been replaced by a CH 2 group or other isostere for sulfur. These analogs can be made via an intramolecular or intermolecular displacement, using methods known in the art as shown below:

wherein p is 1 or 2. One of skill in the art will readily appreciate that this displacement can also occur using other homologs of the a-amino-g-butyric acid derivative shown above and homocysteine (see, e.g., Frank A. Robey, Meth. in Mol. Bio., 35(6):73-90 (1990).

Alternatively, the amino-terminus of the peptide can be capped with an alpha-substituted acetic acid, wherein the alpha substituent is a leaving group, such as an a-haloacetic acid, for example, a-chloroacetic acid, a-bromoacetic acid, or a-iodoacetic acid. The compounds of the present invention can be cyclized or dimerized via displacement of the leaving group by the sulfur of the cysteine or homocysteine residue. See, e.g., Andreu, et al., Meth. in Mol. Bio., 35(7):91-169 (1994); Barker, et al., J. Med. Chem., 35:2040-2048 (1992) and Or, et al., J. Org. Chem., 56:3146-3149 (1991), each of which is incorporated herein by reference. Examples of dimerized compounds are provided in Tables 7, 9, and 10.

V. Utility

The compounds of the invention are useful in vitro as unique tools for understanding the biological role of TPO, including the evaluation of the many factors thought to influence, and be influenced by, the production of TPO and the receptor binding process. The present compounds are also useful in the development of other compounds that bind to and activate the TPO-R, because the present compounds provide important information on the relationship between structure and activity that should facilitate such development.

The compounds are also useful as competitive binders in assays to screen for new TPO receptor agonists. In such assay embodiments, the compounds of the invention can be used without modification or can be modified in a variety of ways; for example, by labeling, such as covalently or non-covalently joining a moiety which directly or indirectly provides a detectable signal. In any of these assays, the materials thereto can be labeled either directly or indirectly. Possibilities for direct labeling include label groups such as: radiolabels such as 125 I, enzymes (U.S. Pat. No. 3,645,090) such as peroxidase and alkaline phosphatase, and fluorescent labels (U.S. Pat. No. 3,940,475) capable of monitoring the change in fluorescence intensity, wavelength shift, or fluorescence polarization. Possibilities for indirect labeling include biotinylation of one constituent followed by binding to avidin coupled to one of the above label groups. The compounds may also include spacers or linkers in cases where the compounds are to be attached to a solid support.

Moreover, based on their ability to bind to the TPO receptor, the peptides of the present invention can be used as reagents for detecting TPO receptors on living cells, fixed cells, in biological fluids, in tissue homogenates, in purified, natural biological materials, etc. For example, by labelling such peptides, one can identify cells having TPO-R on their surfaces. In addition, based on their ability to bind the TPO receptor, the peptides of the present invention can be used in in situ staining, FACS (fluorescence-activated cell sorting), Western blotting, ELISA, etc. In addition, based on their ability to bind to the TPO receptor, the peptides of the present invention can be used in receptor purification, or in purifying cells expressing TPO receptors on the cell surface (or inside permeabilized cells).

The compounds of the present invention can also be utilized as commercial reagents for various medical research and diagnostic uses. Such uses include but are not limited to: (1) use as a calibration standard for quantitating the activities of candidate TPO agonists in a variety of functional assays; (2) use to maintain the proliferation and growth of TPO-dependent cell lines; (3) use in structural analysis of the TPO-receptor through co-crystallization; (4) use to investigate the mechanism of TPO signal transduction/receptor activation; and (5) other research and diagnostic applications wherein the TPO-receptor is preferably activated or such activation is conveniently calibrated against a known quantity of a TPO agonist, and the like.

The compounds of the present invention can be used for the in vitro expansion of megakaryocytes and their committed progenitors, both in conjunction with additional cytokines or on their own. See, e.g., DiGiusto, et al., PCT Publication No. 95/05843, which is incorporated herein by reference. Chemotherapy and radiation therapies cause thrombocytopenia by killing the rapidly dividing, more mature population of megakaryocytes. However, these therapeutic treatments can also reduce the number and viability of the immature, less mitotically active megakaryocyte precursor cells. Thus, amelioration of the thrombocytopenia by TPO or the compounds of the present invention can be hastened by infusing patients post chemotherapy or radiation therapy with a population of his or her own cells enriched for megakaryocytes and immature precursors by in vitro culture.

The compounds of the invention can also be administered to warm blooded animals, including humans, to activate the TPO-R in vivo. Thus, the present invention encompasses methods for therapeutic treatment of TPO related disorders that comprise administering a compound of the invention in amounts sufficient to mimic the effect of TPO on TPO-R in vivo. For example, the peptides and compounds of the invention can be administered to treat a variety of hematological disorders, including but not limited to platelet disorders and thrombocytopenia, particularly when associated with bone marrow transfusions, radiation therapy, and chemotherapy.

In some embodiments of the invention, TPO antagonists are preferably first administered to patients undergoing chemotherapy or radiation therapy, followed by administration of the tpo agonists of the invention.

The activity of the compounds of the present invention can be evaluated either in vitro or in vivo in one of the numerous models described in McDonald, Am. J. of Pediatric Hematology/Oncology, 14:8-21 (1992), which is incorporated herein by reference.

According to one embodiment, the compositions of the present invention are useful for treating thrombocytopenia associated with bone marrow transfusions, radiation therapy, or chemotherapy. The compounds typically will be administered prophylactically prior to chemotherapy, radiation therapy, or bone marrow transplant or after such exposure.

Accordingly, the present invention also provides pharmaceutical compositions comprising, as an active ingredient, at least one of the peptides or peptide mimetics of the invention in association with a pharmaceutical carrier or diluent. The compounds of this invention can be administered by oral, pulmonary, parental (intramuscular, intraperitoneal, intravenous (IV) or subcutaneous injection), inhalation (via a fine powder formulation), transdermal, nasal, vaginal, rectal, or sublingual routes of administration and can be formulated in dosage forms appropriate for each route of administration. See, e.g., Bernstein, et al., PCT Patent Publication No. WO 93/25221; Pitt, et al., PCT Patent Publication No. WO 94/17784; and Pitt, et al., European Patent Application 613,683, each of which is incorporated herein by reference.

Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is admixed with at least one inert pharmaceutically acceptable carrier such as sucrose, lactose, or starch. Such dosage forms can also comprise, as is normal practice, additional substances other than inert diluents, e.g., lubricating agents such as magnesium stearate. In the case of capsules, tablets, and pills, the dosage forms may also comprise buffering agents. Tablets and pills can additionally be prepared with enteric coatings.

Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, with the elixirs containing inert diluents commonly used in the art, such as water. Besides such inert diluents, compositions can also include adjuvants, such as wetting agents, emulsifying and suspending agents, and sweetening, flavoring, and perfuming agents.

Preparations according to this invention for parental administration include sterile aqueous or non-aqueous solutions, suspensions, or emulsions. Examples of non-aqueous solvents or vehicles are propylene glycol, polyethylene glycol, vegetable oils, such as olive oil and corn oil, gelatin, and injectable organic esters such as ethyl oleate. Such dosage forms may also contain adjuvants such as preserving, wetting, emulsifying, and dispersing agents. They may be sterilized by, for example, filtration through a bacteria retaining filter, by incorporating sterilizing agents into the compositions, by irradiating the compositions, or by heating the compositions. They can also be manufactured using sterile water, or some other sterile injectable medium, immediately before use.

Compositions for rectal or vaginal administration are preferably suppositories which may contain, in addition to the active substance, excipients such as cocoa butter or a suppository wax. Compositions for nasal or sublingual administration are also prepared with standard excipients well known in the art.

The compositions containing the compounds can be administered for prophylactic and/or therapeutic treatments. In therapeutic applications, compositions are administered to a patient already suffering from a disease, as described above, in an amount sufficient to cure or at least partially arrest the symptoms of the disease and its complications. An amount adequate to accomplish this is defined as “therapeutically effective dose”. Amounts effective for this use will depend on the severity of the disease and the weight and general state of the patient.

The compositions of the invention can also be microencapsulated by, for example, the method of Tice and Bibi (in Treatise on Controlled Drug Delivery, ed. A. Kydonieus, Marcel Dekker, N.Y. (1992), pp. 315-339).

In prophylactic applications, compositions containing the compounds of the invention are administered to a patient susceptible to or otherwise at risk of a particular disease. Such an amount is defined to be a “prophylactically effective dose”. In this use, the precise amounts again depend on the patient's state of health and weight.

The quantities of the TPO agonist necessary for effective therapy will depend upon many different factors, including means of administration, target site, physiological state of the patient, and other medicants administered. Thus, treatment dosages should be titrated to optimize safety and efficacy. Typically, dosages used in vitro may provide useful guidance in the amounts useful for in situ administration of these reagents. Animal testing of effective doses for treatment of particular disorders will provide further predictive indication of human dosage. Various considerations are described, e.g., in Gilman, et al. (eds), Goodman and Gilman's: The Pharmacological Basis of Therapeutics, 8th ed., Pergamon Press (1990); and Remington's Pharmaceutical Sciences, 7th Ed., Mack Publishing Co., Easton, Pa. (1985); each of which is hereby incorporated by reference.

The peptides and peptide mimetics of this invention are effective in treating TPO mediated conditions when administered at a dosage range of from about 0.001 mg to about 10 mg/kg of body weight per day. The specific dose employed is regulated by the particular condition being treated, the route of administration as well as by the judgement of the attending clinician depending upon factors such as the severity of the condition, the age and general condition of the patient, and the like.

Although only preferred embodiments of the invention are specifically described above, it will be appreciated that modifications and variations of the invention are possible without departing from the spirit and intended scope of the invention.

EXAMPLE 1

Solid Phase Peptide Synthesis

Various peptides of the invention were synthesized using the Merrifield solid phase synthesis techniques (see Steward and Young, Solid Phase Peptide Synthesis, 2d. edition, Pierce Chemical, Rockford, Ill. (1984) and Merrifield, J. Am. Chem. Soc., 85:2149 (1963)) or an Applied Biosystems Inc. Model 431A or 433A peptide synthesizer. The peptides were assembled using standard protocols of the Applied Biosystems Inc. Synth Assist™ 1.0.0 or Synth Assist™ 2.0.2. Each coupling was performed for 2×30 min. with HBTU (2-(1H-benzatriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluoro-phosphate) and HOBt (1-hydroxybenzotriazole).

The resin used was HMP resin (p-hydroxymethyl phenoxymethyl)polystyrene resin or PAL (Milligen/Biosearch), which is a cross-linked polystyrene resin with 5-(4′-Fmoc-aminomethyl-3,5′-dimethyoxyphenoxy) valeric acid as a linker. Use of PAL resin results in a carboxyl terminal amide functionality upon cleavage of the peptide from the resin. Upon cleavage, the HMP resin produces a carboxylic acid moiety at the C-terminus of the final product. Most reagents, resins, and protected amino acids (free or on the resin) were purchased from Millipore or Applied Biosystems Inc.

The Fmoc group was used for amino protection during the coupling procedure. Primary amine protection on amino acids was achieved with Fmoc and side chain protection groups were t-butyl for serine, tyrosine, glutamic acid, and threonine; trityl for glutamine; Pmc (2,2,5,7,8-pentamethylchroman-6-sulfonyl) for arginine; N-t-butyloxycarbonyl for tryptophan; N-trityl for histidine and S-trityl for cysteine.

Removal of the peptides from the resin and simultaneous deprotection of the side chain functions were achieved by treatment with reagent K or slight modifications of it. Alternatively, in the synthesis of those peptides, with an amidated carboxyl terminus, the fully assembled peptide was cleaved with a mixture of 90% trifluoroacetic acid, 5% ethanedithiol, and 5% water, initially at 4° C., and gradually increasing to room temperature. The deprotected peptides were precipitated with diethyl ether. In all cases, purification was by preparative, reverse-phase, high performance liquid chromatography on a C 18 bonded silica gel column with a gradient of acetonitrile/water in 0.1% trifluoroacetic acid. The homogeneous peptides were characterized by Fast Atom Bombardment mass spectrometry or electrospray mass spectrometry and amino acid analysis when applicable.

In a preferred embodiment, the peptides of this invention are dimerized using standard synthetic procedures known to and used by those of skill in the art. Exemplar synthetic schemes for preparing the dimer peptide compounds of this invention are set forth in FIGS. 26 and 27 . Following these synthetic schemes, those of skill in the art can readily prepare dimer peptide compounds in accordance with the present invention. In addition, it will be readily apparent to those of skill in the art that the dimeric subunits can readily be linked using methodologies and linkers other than those described in FIGS. 26 and 27 .

Pegylation of the Peptides

A. Preparation of di-PEGylated AF13948 with PEG2 Branched PEG, 20,000 Mw (see, FIG. 15 )

AF13948 was dissolved in 100 mM bicine pH 8.0 at a concentration of 10 mg/ml, added to a 1.25 fold molar excess of powdered PEG2 (commercially available from Shearwater Polymers, Inc. (Huntsville, Ala.)) and stirred at room temperature until the reaction was complete, typically 1-2 hours. The reaction was monitored by reverse phase HPLC using a 40-65% acetonitrile gradient with a YMC ODS AQ column. When the reaction was complete, the solution was added to a second 1.25 molar excess of powdered PEG2 and the process was repeated 4 times using a total of 5 moles of PEG2 for each mole of AF13948. The solution was diluted 2 fold with PBS to reduce the viscosity and loaded onto a superdex 200 column (Pharmacia), previously equilibrated and eluted with PBS. Fractions from the size exclusion column were analyzed by reverse phase HPLC. Fractions containing di-PEG-AF13948 which eluted prior to any mono-PEG-AF13948 were pooled and stored at 5° C. or lyophilized.

B. Preparation of di-PEGylated AF13948 with SPA-mPEG 20,000 Mw (see, FIG. 16 )

AF13948 was dissolved in 100 mM bicine pH 8.0 at a concentration of 10 mg/ml, added to a 5 fold molar excess of powdered SPA-mPEG (commercially available from Shearwater Polymers, Inc. (Huntsville, Ala.)) and stirred at room temperature until the reaction was complete, typically 2 hours. The reaction was monitored by reverse phase (YMC ODS AQ) HPLC and when it was complete, the solution was diluted 10 fold with deionized water and loaded onto a SP-sepharose column equilibrated in 2 mM sodium phosphate buffer at pH 7.0. The unreactded PEG and the NHS flowed through the column. The PEGylated AF13948 was then eluted using a 0 to 150 mM NaCl gradient, with the di-PEGylated form eluting before any mono-PEGylated material. The fractions were analyzed by reverse phase HPLC and the appropriate fractions combined. The di-PEG-AF13948 was buffer exchanged into PBS and either stored at 5° C. or lyophilized.

C. Preparation of di-PEGylated AF13848 with mPEG Aldehyde 20,000 Mw (see, FIG. 17 )

AF13948 was dissolved in 100 mM sodium phosphate pH 7.0 at a concentration of 10 mg/ml, added to a 6 fold molar excess of powdered PEG aldehyde (commercially available from Shearwater Polymers, Inc. (Huntsville, Ala.)) and 1M sodium cyanoborohydride solution was added to give a final sodium cyanoborohydride concentration of 100 mM. The solution was stirred for 18 hours at room temperature, then buffer exchanged into PBS, using a 3K (Amicon YM) ultrafiltration membrane. The solution ws diluted 2 fold with PBS to reduce the viscosity and loaded on a superdex 200 column (Pharmacia), previously equilibrated and eluted with PBS. Fractions from the size exclusion column were analyzed by reverse phase HPLC. Fractions containing di-PEG-AF13948 which eluted prior to any mono-PEG-AF13948 were pooled and stored at 5° C. or lyophilized.

EXAMPLE 2

Bioassays

Bioactivity of the peptides can be measured using a thrombopoietin dependent cell proliferation assay. Murine IL-3 dependent Ba/F3 cells were transfected with full length human TPO-R. In the absence of IL-3 (WEHI-3 conditioned media), these cells are dependent on TPO for proliferation. The parental, untransfected cell line does not respond to human TPO, but remains IL-3 dependent.

Bioassays have been performed on both of the above cell lines using synthetic peptides derived from library screening. The cells were grown in complete RPMI-10 media, containing 10% WEHI-3 conditioned media, then washed twice in PBS, resuspended in media which lacked WEHI-3 conditioned media, and added to wells containing dilutions of peptide or TPO at 2×10 4 cells/well. The cells were incubated for 48 hours at 37° C. in a humidified 5% CO 2 atmosphere and metabolic activity was assayed by the reduction of MTT to formazan, with absorbance at 570 Nm measured on an ELISA plate reader. The peptides tested stimulated proliferation of TPO-R transfected Ba/F3 cells in a dose dependent manner as shown in FIG. 1 . These peptides have no effect on the parental cell line.

EXAMPLE 3

Binding Affinity

Binding affinities of chemically synthesized peptides for TPO-R were measured in a competition binding assay. The wells of a microtiter plate were coated with 1 mg streptavidin, blocked with PBS/1% BSA, followed by 50 ng of biotinylated anti-receptor immobilizing antibody (Ab179). The wells were then treated with a 1:10 dilution of soluble TPO-R harvest. Various concentrations of peptide or peptide mimetic were mixed with a constant amount of a truncated form of TPO consisting of residues 1-156 fused to the C-terminus of maltose binding protein (MBP-TPO 156 ). The peptide MBP-TPO 156 mixtures were added to the TPO-R coated wells, incubated for 2 hours at 4° C. and then washed with PBS. The amount of MBP-TPO 156 that was bound at equilibrium was measured by adding a rabbit anti-sera directed against MBP, followed by alkaline phosphatase conjugated goat anti-rabbit IgG. The amount of alkaline phosphatase in each well was then determined using standard methods.

The assay is conducted over a range of peptide concentrations and the results are graphed such that the y axis represents the amount of bound MBP-TPO 156 and the x axis represents the concentration of peptide or peptide mimetic. One can then determine the concentration at which the peptide or peptide mimetic will reduce by 50% (IC 50 ) the amount of MBP-TPO 156 bound to immobilized TPO-R. The dissociation constant (Kd) for the peptide should be similar to the measured IC 50 using the assay conditions described above.

EXAMPLE 4

Peptides on Plasmids

The pJS142 vector is used for library construction and is shown in FIG. 4 . Three oligonucleotide sequences are needed for library construction: ON-829 (5′ ACC ACC TCC GG); ON-830 (5′ TTA CTT AGT TA) and a library specific oligonucleotide of interest (5′ GA GGT GGT {NNK} n TAA CTA AGT AAA GC), where {NNK} n denotes a random region of the desired length and sequence. The oligonucleotides can be 5′ phosphorylated chemically during synthesis or after purification with polynucleotide kinase. They are then annealed at a 1:1:1 molar ratio and ligated to the vector.

The strain of E. coli which is preferably used for panning has the genotype: Δ(srl-recA) endA1 nupG lon-11 sulA1 hsdR17 Δ(ompT-fepC)266 ΔclpA319::kan ΔlacI lac ZU118 which can be prepared from an E. coli strain from the E. coli Genetic Stock Center at Yale University ( E. coli b/r, stock center designation CGSC:6573) with genotype lon-11 sulA1. The above E. coli strain is prepared for use in electroporation as described by Dower, et al., Nucleic Acids Res., 16:6127 (1988), except that 10% glycerol is used for all wash steps. The cells are tested for efficiency using 1 pg of a Bluescript plasmid (Stratagene). These cells are used for growth of the original library and for amplification of the enriched population after each round of panning.

Peptides on plasmids are released from cells for panning by gentle enzymatic digestion of the cell wall using lysozyme. After pelleting of the cell debris, the crude lysate can be used directly on most receptors. If some additional purification of the plasmid complexes is needed, a gel filtration column can be used to remove many of the low molecular weight contaminants in the crude lysate.

Panning is carried out in a buffer (HEKL) of a lower salt concentration than most physiological buffers. The panning can be conducted in microtiter wells with a receptor immobilized on a nonblocking monoclonal antibody (MAb) or by panning on beads or on columns. More specifically, in the first round of panning, 24 wells, each coated with receptor, can be used. For the second round, six wells coated with receptor (PAN sample) and 6 wells without receptor (NC sample) are typically used. Comparison of the number of plasmids in these two samples can give an indication of whether receptor specific clones are being enriched by panning. “Enrichment” is defined as the ratio of PAN transformants to those recovered from the NC sample. Enrichment of 10 fold is usually an indication that receptor specific clones are present.

In later rounds of panning, it is useful to reduce the input of lysate into the wells to lower nonspecific background binding of the plasmid complexes. In round 2, usually 100 μl of lysate per well is used. In round 3, 100 μl of lysate per well diluted with 1/10 in HEKL/BSA is used. For further rounds of panning, typically an input of plasmid transforming units of at least 1000 fold above the estimated remaining diversity is used.

The binding properties of the peptides encoded by individual clones are typically examined after 3, 4, or 5 rounds of panning, depending on the enrichment numbers observed. Typically, an ELISA that detects receptor specific binding by LacI-peptide fusion proteins is used. LacI is normally a tetramer and the minimum functional DNA binding species is a dimer. The peptides are thus displayed multivalently on the fusion protein. Assuming that a sufficient density of receptor can be immobilized in wells, the peptides fused to LacI will bind to the surface in a cooperative, multivalent fashion. This cooperative binding permits the detection of binding events of low intrinsic affinity. The sensitivity of this assay is an advantage in that initial hits of low affinity can be easily identified, but is a disadvantage in that the signal in the ELISA is not correlated with the intrinsic affinity of the peptides. Fusion of the peptides to maltose binding protein (MBP) as described below permits testing in an ELISA format where signal strength is better correlated with affinity. See FIGS. 5A-B .

DNA from clones of interest can be prepared in double stranded form using any standard miniprep procedure. The coding sequences of interesting single clones or populations of clones can be transferred to vectors that fuse those sequences in frame with the gene encoding MBP, a protein that generally occurs as a monomer in solution. The cloning of a library into PJS142 creates a BspEI restriction site near the beginning of the random coding region of the library. Digestion with BspEI and ScaI allows the purification of a ˜900 bp DNA fragment that can be subcloned into one of two vectors, pELM3 (cytoplasmic) or pELM15 (periplasmic), which are simple modifications of the pMALc2 and pMALp2 vectors, respectively, available commercially from New England Biolabs. See FIGS. 5A-B . Digestion of pELM3 and pELM15 with AgeI and ScaI allows efficient cloning of the BspEI-ScaI fragment from the pJS142 library. The BspEI and AgeI ends are compatible for ligation. In addition, correct ligation of the ScaI sites is essential to recreate a functional bla (Amp resistance) gene, thus lowering the level of background clones from undesired ligation events. Expression of the tac promoter-driven MBP-peptide fusions can then be induced with IPTG.

Lysates for the LacI or MBP ELISAS are prepared from individual clones by lysing cells using lysozyme and removing insoluble cell debris by centrifugation. The lysates are then added to wells containing immobilized receptor and to control wells without receptor. Binding by the LacI or MBP peptide fusions is detected by incubation with a rabbit polyclonal antiserum directed against either LacI or MBP followed by incubation with alkaline phosphatase labeled goat anti rabbit second antibody. The bound alkaline phosphatase is detected with p-nitrophenyl phosphate chromogenic substrate.

EXAMPLE 5

“Headpiece Dimer” System

A variant of the LacI peptides-on-plasmids technique utilizes a DNA binding protein called “headpiece dimer”. DNA binding by the E. coli lac repressor is mediated by the approximately 60 amino acid “headpiece” domain. The dimer of the headpiece domains that binds to the lac operator is normally formed by association of the much larger approximately 300 amino acid C-terminal domain. The “headpiece dimer” system utilizes headpiece dimer molecules containing two headpieces connected via short peptide linker. These proteins bind DNA with sufficient stability to allow association of a peptide epitope displayed at the C-terminus of the headpiece dimer with the plasmid encoding that peptide.

The random peptides are fused to the C-terminus of the headpiece dimer, which binds to the plasmid that encoded it to make a peptide-headpiece dimer-plasmid complex that can be screened by panning. The headpiece dimer peptides-on-plasmids system allows greater selectivity for high affinity ligands than the LacI system. Thus, the headpiece dimer system is useful for making mutagenesis libraries based on initial low-affinity hits, and selecting higher affinity variants of those initial sequences.

The libraries are constructed as with peptides on plasmids using headpiece dimer vector pCMG14 (see FIGS. 6 A-C). The presence of the lac operator is not required for plasmid bindimg by the headpiece dimer protein. The libraries were introduced into bacterial strain comprising E. coli (lon-11 su1A1 hsdR17 (ompT-fepC) ΔclpA319::kan ΔlacI lac ZU118 Δ(srl-recA) 306::Tn10 and amplified under conditions of basal (A) promoter induction. Panning of headpiece dimer libraries is carried out by similar procedures to those used for LacI libraries, except that HEK buffer is used instead of HEKL buffer and elution of plasmids from the wells is performed with aqueous phenol instead of with IPTG. Sequences from headpiece dimer panning are often characterized after transfer to the MBP vector so that they can be tested in the affinity sensitive MBP ELISA and also so that populations of clones can be screened by colony lifts with labeled receptor.

EXAMPLE 6

In this example cyclized compounds were subjected to three assays. First, IC 50 valves were obtained as described above. Additionally, an MTT cell proliferation assay as described above was performed to calculate EC 50 values. Finally, a microphysiometer (Molecular Devices Corp.) assay was performed. Basically, in this assay the rate of acidification of the extracellular medium in response to TPO receptor stimulation by the compounds of the invention was determined. The ranges for EC 50 are symbolically indicated as for IC 50 described above. The results are summarized in Table 4.

TABLE 4
	EC50 (nM)	EC50 (nM)
Structure	Proliferation	Microphys.	IC50 (nM)
	++	++	++
	++	++	++
	++	++	ND
	+	+
	+	+	ND
	+	+	++
	+	+	++
	+	+	++
	+	+	++
	+	+	+
	++	+	++
	+	+	ND
	+	+	ND
	++	+	ND
	++	+	ND
	+	+	+
	+	+	ND

EXAMPLE 7

In this example amino acid substitutes at positions D, E, I, S, or F in the cyclized compound

were assayed for EC 50 and IC 50 values as described above. Microphysiometer results are given in parentheses. The results are summarized in Table 5 below.

TABLE 5
		EC50 (nM)
	Substitution	Cell Prolif.	IC50 (nM)
	E-Q	++(+)	++
	D-A	+(+)	++
	I-A	+(+)	+
	S-A	++(++)	++
	S-D-Ala	+	+
	S-Sar	+	++
	S-Aib	++(+)	++
	S-D-Ser	++	++
	S-Nva	++(++)	++
	S-Abu	++	++
	S-(N—Me-Ala)	+	+
	S-(N—Me-Val)	+	+
	S-(N—Me-Ala)*	+	+
	S-(Nor-Leu)	++	++
	S-(t-Bu-Gly)	+	++
	S-[N—Me-Ser(Bzl)]		+
	S-(Homoser)	ND	ND
	S-(N—Me-Leu)	+	ND
	F-A	+(+)	++
	F-D-Ala	+	++
	F-D-Phe	+	++
	F-Homo-Phe	++(++)	++
	F-CHA	++(++)	++
	F-Thi	++	++
	F-(Ser(Bzl))	++	++
	F-(N—Me-Ala)	+	+
	F-(Phenylgly)	++(++)	++
	F-(Pyridylala)	++	++
	F-(p-Nitrophe)	++(++)	++
	F-(3,4-di-Cl-Phe)	++(+)	++
	F-(p-Cl-Phe)	++	++
	F-(2-Nal)	++(++)	++
	F-(1-Nal)	++	++
	F-(DiPh-Ala)	++	++
	F-(N—Me-Phe)	++	ND
	S,F-Ava(thioether)	+	++
	S,F-Ava(cys-cys)	+	++
	S,F-Ava	+	++
	AD-deletion	+(+)	ND
	ADG-deletion	(+)	+

EXAMPLE 8

In this example, amino acid substitutions in the compound

were evaluated at positions D, S, or F as indicated in Table 6 below. EC 50 and IC 50 values were calculated as described above. Microphysiometer results are in parentheses.

TABLE 6
		EC50 (nM)
	Substitution	Cell Prolif.	IC50 (nM)
	D-E	(+)	ND
	free acid form	++(+)	ND
	C-term. Gly addition	++	++
	S-Abu	++(++)	ND
	F-DiPh-Ala	(++)	++
	S,F-Abu,DiPh-Ala	+(+)	++

EXAMPLE 9

In this example EC 50 and IC 50 values were calculated as described above for the dimer compounds listed in Table 7 below. The cyclized monomer

is included as a comparison.

In Table 8, EC 50 and IC 50 values determined as described above for cyclized and dimerized variants of I E G P T L R Q W L A A R A are compared.

In Table 9, truncations of the dimer

are compared. EC 50 and IC 50 values were calculated as described above. Microphysiometer results are given in parentheses.

It should be noted that in the dimerized peptides compounds set forth in, for example, Tables 9, 13 and 14, each of the dimeric subunits is attached to a lysine linker, with one of the dimeric subunits being attached to the α-amino group of the lysine and the other dimeric subunit being attached to the ε-amino group of the lysine.

	TABLE 7
	EC50 (nM)
	Micro-		IC50
	phys.	Prolif.	(nM)
	++	++	++
	++	++	++
	++	++	++
	++	++	++
	++	++	++
	ND	+	+
	++	++	++
	ND	++	++
	++	++	++
	++	++	++
	ND	++	++
	++	++	+
	ND	++	++
	++	++	+
	+	+	+
	++	+	+

	TABLE 8
	EC50 (nM)
	Micro-		IC50
	phys.	Prolif.	(nM)
(H)-IEGPTLRQWLAARA-(NH 2 )	N.D.	++	++
	N.D.	++	++
	++	++	++
	++	++	++

	TABLE 8
	EC50 (nM)
	Micro-		IC50
	phys.	Prolif.	(nM)
(H)-IEGPTLRQWLAARA-(NH 2 )	N.D.	++	++
	N.D.	++	++
	++	++	++
	++	++	++

EXAMPLE 10

In this example various substitutions were introduced at positions G, P, and W in the cyclized compound

Table 10 lists examples of the substituted compounds that show TPO agonist activity. The substitutions abbreviated in the table are as follows:

TABLE 10
G	P	W
Sar	Hyp(OBn)	Nal
Sar	Hyp(OBn)	Nal
Gly	Pro	Trp
Gly	Pro	Trp
Sar	Hyp(Obn)	Nal
Gaba	Pro	Trp
Cpr-Gly	Pro	Trp
Sar	Hyp(OBn)	Nal
Gly	Pro	Trp
Gly	Pro	Nal
Sar	Pro	Trp
Cpr-Gly	L-Tic	Nal
Gly	D-Tic	D-Trp
Cpr-Gly	D-Tic	Trp
Gaba	Hyp(OBn)	Trp

EXAMPLE 11

To assess the feasibility of mice as a convenient test species, several in vitro experiments, designed to measure the activity of the test compounds on the mouse receptor, have been done. First, marrow cells, harvested from the femurs of 8 to 9 week one Balb/C mice, were incubated for 7 days in liquid culture with either rhuTPO or various concentrations of the test peptides. At the end of the incubation period, the cultures were concentrated by Cytospin, stained for acetylcholinesterase (AChE, a diagnostic of mouse megakaryocytes), and counted by microscopic analysis. One (1) Nm rhuTPO gave rise to the outgrowth of very large (>40 μm) non-adherent cells that stain for AChE. These cells appear to be mature megakaryocytes. From an initial seeding of 10 6 total marrow cells/ml (in 50 ml cultures) an estimated 1 to 2×10 6 megakaryocytes developed. This response to TPO was designated as “maximal”. Control cultures containing no added growth factors produced very few AChE-positive cells. Several of the peptide compounds were tested at high concentration in this assay and the results are summarized in Table 11. Peptide A at 10 uM produced a maximal response of the mouse marrow. This finding was the first evidence that this peptide family is active on the murine receptor. In a second experiment, marrow cells were harvested and cultured in semi-solid medium (methylcellulose) containing either no factors, 1 nM rhuTPO, or 10 uM Peptide A. After 7 days in culture, colonies of large cell (presumed to be megakaryocytes) were counted and grouped into small colonies (3-5 cells) or large colonies (greater than 6 cells). The results are shown in Table 12. TPO and the test peptides both produced substantially more colonies of both size than did the negative control cultures. This indicates that the peptides mimic TPO in their ability to stimulate the expansion of the Mk precursor cell population.

To obtain a more quantitative comparison of the activity of the test compounds on murine and human receptors, the muTPO receptor was cloned and transfected into BaF3 cells. A TPO dependent population of cells was isolated.

	TABLE 11
	Peptide	Concentration Tested (nM) Response
	D	100,000	none
	C	40,000	maximal**
	C + S.A.*	1000	maximal**
	S.A. alone	1000	none
	B	100,000	minimal
	A	10,000	maximal**
	TPO (R & D)	1	“maximal”
	*Streptavidin complexed to biotinylated peptide - concentration of putative 1:4 complex.
	**Compared to recombinant human TPO
	**25-30% ACE staining cells on cytospin
	No factor cultures - ca. 5% AChE staining cells (lower cellularity)

	TABLE 11
	Peptide	Concentration Tested (nM) Response
	D	100,000	none
	C	40,000	maximal**
	C + S.A.*	1000	maximal**
	S.A. alone	1000	none
	B	100,000	minimal
	A	10,000	maximal**
	TPO (R & D)	1	“maximal”
	*Streptavidin complexed to biotinylated peptide - concentration of putative 1:4 complex.
	**Compared to recombinant human TPO
	**25-30% ACE staining cells on cytospin
	No factor cultures - ca. 5% AChE staining cells (lower cellularity)

EXAMPLE 12

In this example, various substitutions, deletions and additions to the dimer

were analyzed using three assays. First, an MTT cell proliferation assay was performed as described above. Second, a microphysiometer assay was performed as described above. Finally, a reporter assay was performed. BaF3/TPOR cells transfected with a c-fos/luciferase reporter plasmid were starved overnight in complete RPMI-10 media containing 0.1% WEHI-3 conditioned media, then washed two times in PBS. Cells were resuspended in media which lacked WEHI-3 conditioned media, and added to wells containing serial dilutions of peptide at 5×10 5 cells/well. The cells were incubated for two hours at 37° in a humidified 5% CO 2 atmosphere, and luciferase expression was measured with a luminometer after addition of the luciferin substrate.

TABLE 13
	EC50
	(pM)	EC50
	Cell	(pM)	EC50 (pM)
Peptide Sequence	Prolif.	Reporter	Microphys
	++	++	++
	++	++	++
	++	++	++
	++	++	++
	++	++	ND
	++	++	ND
	++	++	ND
	++	++	++
	++	++	ND
	++	++	++
	++	++	ND
	++	++	++
	ND	++	ND
	ND	++	++
	++	++	++
	ND	++	ND
	++	++	++
	ND	++	ND
	ND	++	ND
	ND	++	ND
	++	++	ND
	++	ND	ND

TABLE 14
	EC50
	(nM)
	Cell	IC50
Peptide Sequence	Prolif.	(nM)
	++	++
	++	++
	++	++
	++	++
	++	++
(H)-ADGPTLREWISF(Ava)ADGPTLREWISF(NH 2 )	++	ND
	++	ND

EXAMPLE 13

In this example, the pharmacokinetic behavior of various PEGylated peptides was determined. Pharmacokinetic behavior of compounds is an important determinant of their pharmacological activity. A key component of the pharmacokinetic profile is the persistence of the compound in the plasma of laboratory animals. This persistence is usually expressed in terms of compound concentration in plasma as a function of time after administration.

Throughout these experiments, male 20-25 g Balb/c mice were used. A volume of 200 μl was injected IV or SC. The vehicle was Dulbecco's PBS; 5% DMSO; 0.1% w/v BSA. Plasma was harvested at sacrifice using heparin as anticoagulant.

Compound concentrations were measured using a reporter cell assay. Dilutions of plasma were added to the Baf/3 TPOr/fos/lux construct 3. Luciferase expression was measured with a luminometer after addition of the luciferin substrate. The stimulatory activity of the individual plasma samples was converted to a concentration expressed as peptide equivalents of compound per volume of plasma (nM or ng/ml). This concentration was established by comparison with a standard curve constructed in the reporter assay with the parent compound.

Table 15 and FIG. 10 show the concentrations in plasma of compounds AF13948 and the di-pegylated AF13948 (polyethylene glycol (PEG) average MW=5000D) as a function of time after injection of 700 μg peptide/kg. Administration of AF13948 results in activity in plasma detectable above the level present in vehicle-injected mice until 60 min PI. The addition of the 5K PEG increases the concentration in plasma greater than 100-fold and extends the time it can be detected in plasma until at least 240 min PI.

TABLE 15
Plasma concentrations of AF13948, 5K DiPEG-AF13948
following IV injection of 700 μg/kg
expressed as peptide equivalents (ng/ml)
	time (min)	DiPEG-AF13948	AF13948	vehicle
	10	361.36	0.80	0.02
	30	91.49	0.26	0.02
	60	54.59	0.11	0.02
	120	16.54	0.04	0.03
	240	11.20	0.02	0.01

Table 16 and FIG. 11 show the concentrations in plasma of compounds 5K DiPEG AF13948 (PEG average MW=5000D) and the 20K DiPEG AF13948 (PEG average MW=20,000D) as a function of time after injection of 500 μg peptide/kg. Concentration and persistence in plasma of the 20K PEG-modified compound is greatly increased above that of the 5K DiPEG AF13948.

TABLE 16
Plasma concentrations of AF13948, 5K DiPEG-AF13948
following IV injection of 500 μg/kg
expressed as peptide equivalents (ng/ml)
	time (hours)	5K	20K	vehicle
	0.5	54.56	1257.50	0.04
	1.5	16.89	3481.31	0.03
	4	2.76	1970.46	0.02
	8	1.02	1158.59	0.02
	16	0.17	259.41	0.03
	24	0.13	710.75	0.04

Table 17 and FIG. 12 show the plasma concentrations of compound 20K DiPEG AF13948 after injection of 100, 10 and 1 μg peptide/kg and extends the time of observation to 120 hours PI. Concentrations observed in plasma were found to be proportional to administered doses. Administration of a single IV dose of 100 μg/kg of the compound resulted in elevated plasma concentrations for at least 96 hours PI.

TABLE 17
Plasma concentrations of 20K DiPEG-AF13948 peptide
following IV injection of 1, 10, or 100 μg/kg
expressed as nM DiPEG-13948 peptide equivalents
	time (hours)	dose (μg/kg)
	0.08	291.66	32.89	2.93
	0.25	550.82	45.48	2.63
	0.5	1318.53	29.16	2.33
	1.5	328.30	39.12	0.98
	3	285.43	25.77	1.39
	8	134.60	8.33	0.90
	24	106.36	11.79	0.20
	48	56.64	0.70	0.06
	72	15.19	0.57	0.01
	96	0.42	0.01	0.00
	120	0.02	0.01	0.00

Table 18 and FIG. 13 show the plasma concentrations following the SC and IV injection of 10 μg/kg 20K DiPEG AF13948. SC injection of a single 10 μg/kg dose resulted in elevated plasma activities for 96 hours PI. Profiles of plasma concentrations achieved with these 2 routes of administration indicate the good bioavailability of SC administered 20K DiPEG AF13948.

TABLE 18
Plasma concentrations of 20K DiPEG-AF13948 peptide
following IV and SC injection of 10 μg/kg
expressed as nM DiPEG-13948 peptide equivalents
time
(hours)	SC	IV
0.08	0.02	32.89
0.25	0.06	45.48
0.5	0.09	29.16
1.5	0.36	39.12
3	0.82	25.77
8	1.48	8.33
24	7.27	11.79
48	1.09	0.70
72	0.03	0.57
96	0.01	0.01
120	0.01	0.01

In addition, FIG. 14 indicates that GW350781, 5K-PEGylated peptide, has increased stability, i.e., an increased half-life, over the non-PEGylated form of the peptide. As such, FIGS. 10-14 indicate that the PEGylated peptides have good bioavailability and increased stability in human serum.

EXAMPLE 14

Using the assay described above, the pharmacokinetic profile of a peptide variously derivatized with PEG was determined. In this experiment, the peptide was derivatized with branched PEG (di(2)), with an ester linked PEG (SPA) and with an aldehyde linked PEG (ALDH) as illustrated in FIGS. 15-17 . FIG. 18 shows the plasma concentrations following SC injection of 10 g/kg. From FIG. 18 , it is apparent that all three of the peptide compounds variously derivatized PEG have favorable pharmacokinetic profiles.

EXAMPLE 15

In this experiment, PEGylated peptides were evaluated for their effect on thrombocytopenia in a mouse model. In this assay, mice are made thrombocytopenic by treating the Balb/C mice with carboplatin (90 mg/kg intraperitoneally) on Day 0. GW35071 (1 mg/kg/day), the 5K-PEGylated peptide, and GW305805 (32.5 μg/kg/day), the 20K-diPEGylated peptide, were given on Days 1-9 (s.c., qd). From FIGS. 19-20 , it is apparent that the PEGylated peptides ameliorate carboplatin-induced thrombocytopenia on about Day 10. These results clearly indicate that the PEGylated peptides of the invention can ameliorate thrombocytopenia in a mouse model.

EXAMPLE 16

In this experiment, PEGylated peptides were evaluated for their effect on platelet levels in normal mice. In one experiment, GW350781 (1 mg/kg/day), the 5K-PEGylated peptide, and GW305805 (32.5 μg/kg/day), the 20K-diPEGylated peptide, were given on Days 1-9 (s.c., qd). On days 1-15, blood was sampled at intervals by tail vein bleeds. From FIGS. 21-22 , it is apparent that the PEGylated peptides have an effect on thrombocytosis, with the 20K-diPEGylated peptide being about 100-fold more potent than the 5K-PEGylated peptide.

In another experiment, GW350781, the 5K-PEGylated peptide, and GW305805, the 20K-diPEGylated peptide, were given on Days 1-5 (s.c., qd). On day 6, the animals were sacrificed and peripheral blood platelet counts were obtained. FIGS. 23-25 set forth the effects of varying doses of the PEGylated peptides as well as the effects of single-dose versus multiple-dose. Such results clearly indicate that the PEGylated peptides of the invention can be used to increase platelets in a mouse model.

The disclosures in this application of all articles and references, including patent documents, are incorporated herein by reference in their entirety for all purposes.

244 5 amino acids amino acid <Unknown> linear peptide not provided 1Trp Ser Xaa Trp Ser1 5 7 amino acids amino acid <Unknown> linear peptide not provided 2Xaa Xaa Xaa Xaa Xaa Xaa Xaa1 5 9 amino acids amino acid <Unknown> linear peptide not provided 3Xaa Gly Xaa Xaa Xaa Xaa Xaa Trp Xaa1 5 6 amino acids amino acid <Unknown> linear peptide not provided 4Pro Thr Leu Arg Xaa Xaa1 5 10 amino acids amino acid <Unknown> linear peptide not provided 5Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Trp Xaa1 5 10 18 amino acids amino acid <Unknown> linear peptide not provided 6Gly Gly Cys Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 15Gly Gly 19 amino acids amino acid <Unknown> linear peptide not provided 7Gly Asn Ala Asp Gly Pro Thr Leu Arg Gln Trp Leu Glu Gly Arg Arg1 5 10 15Pro Lys Asn 19 amino acids amino acid <Unknown> linear peptide not provided 8Gly Gly Cys Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 15Gly Gly Lys 18 amino acids amino acid <Unknown> linear peptide not provided 9Thr Ile Lys Gly Pro Thr Leu Arg Gln Trp Leu Lys Ser Arg Glu His1 5 10 15Thr Ser 18 amino acids amino acid <Unknown> linear peptide not provided 10Ser Ile Glu Gly Pro Thr Leu Arg Glu Trp Leu Thr Ser Arg Thr Pro1 5 10 15His Ser 19 amino acids amino acid <Unknown> linear peptide not provided 11Leu Ala Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu His Gly Asn Gly1 5 10 15Arg Asp Thr 14 amino acids amino acid <Unknown> linear peptide not provided 12Cys Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 13Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Ala1 5 10 7 amino acids amino acid <Unknown> linear peptide not provided 14Cys Xaa Xaa Xaa Xaa Xaa Xaa1 5 16 amino acids amino acid <Unknown> linear peptide not provided 15Gly Gly Cys Thr Leu Arg Glu Trp Leu His Gly Gly Phe Cys Gly Gly1 5 10 15 8 amino acids amino acid <Unknown> linear peptide not provided 16Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa1 5 15 amino acids amino acid <Unknown> linear peptide not provided 17Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Ala Lys1 5 10 15 16 amino acids amino acid <Unknown> linear peptide not provided Modified-site 15 /product= “Beta-ala” 18Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Ala Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “1-Nal” Modified-site 14 /product= “Sar” 19Ile Glu Gly Pro Thr Leu Arg Gln Xaa Leu Ala Ala Arg Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “1-Nal” Modified-site 13 /product= “Ac-Lys” Modified-site 14 /product= “Sar” 20Ile Glu Gly Pro Thr Leu Arg Glu Xaa Leu Ala Ala Xaa Xaa Lys1 5 10 15 18 amino acids amino acid <Unknown> linear peptide not provided 21Xaa Xaa Xaa Xaa Xaa Thr Leu Arg Glu Trp Leu Xaa Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa 19 amino acids amino acid <Unknown> linear peptide not provided 22Xaa Xaa Xaa Xaa Xaa Xaa Thr Leu Arg Glu Trp Leu Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa Xaa 18 amino acids amino acid <Unknown> linear peptide not provided 23Xaa Xaa Xaa Xaa Xaa Thr Leu Arg Glu Trp Leu Xaa Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa 18 amino acids amino acid <Unknown> linear peptide not provided 24Xaa Xaa Xaa Xaa Xaa Thr Leu Arg Glu Phe Leu Xaa Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa 10 amino acids amino acid <Unknown> linear peptide not provided 25Arg Glu Gly Pro Thr Leu Arg Gln Trp Met1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 26Arg Glu Gly Pro Thr Leu Arg Gln Trp Met1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 27Ser Arg Gly Met Thr Leu Arg Glu Trp Leu1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 28Glu Gly Pro Thr Leu Arg Gly Trp Leu Ala1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 29Arg Glu Gly Gln Thr Leu Lys Glu Trp Leu1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 30Glu Arg Gly Pro Phe Trp Ala Lys Ala Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 31Arg Glu Gly Pro Arg Cys Val Met Trp Met1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 32Cys Ser Gly Leu Thr Leu Arg Glu Trp Leu Val Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 33Cys Leu Thr Gly Pro Phe Val Thr Gln Trp Leu Tyr Glu Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 34Cys Gly Glu Gly Leu Thr Leu Thr Gln Trp Leu Glu His Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 35Cys Arg Ala Gly Pro Thr Leu Leu Glu Trp Leu Thr Leu Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 36Cys Arg Ala Gly Pro Thr Leu Leu Glu Trp Leu Thr Leu Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 37Cys Arg Gln Gly Pro Thr Leu Thr Ala Trp Leu Leu Glu Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 38Cys Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 39Cys Glu Leu Val Gly Pro Ser Leu Met Ser Trp Leu Thr Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 40Cys Gly Thr Glu Gly Pro Thr Leu Ser Thr Trp Leu Asp Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 41Cys Asp Gln Leu Gly Val Thr Leu Ser Arg Trp Leu Glu Cys1 5 10 18 amino acids amino acid <Unknown> linear peptide not provided 42Ser Gly Thr Gly Leu Thr Leu Arg Glu Trp Leu Gly Ser Phe Ser Leu1 5 10 15Leu Ser 18 amino acids amino acid <Unknown> linear peptide not provided 43Cys Pro Glu Gly Pro Thr Leu Leu Gln Trp Leu Lys Arg Gly Tyr Ser1 5 10 15Ser Cys 18 amino acids amino acid <Unknown> linear peptide not provided 44Arg Gly Asp Gly Pro Thr Leu Ser Gln Trp Leu Tyr Ser Leu Met Ile1 5 10 15Met Cys 18 amino acids amino acid <Unknown> linear peptide not provided 45Met Val Ala Gly Pro Thr Leu Arg Glu Phe Ile Ala Ser Leu Pro Ile1 5 10 15His Cys 18 amino acids amino acid <Unknown> linear peptide not provided 46Ser Met Gln Gly Pro Thr Phe Arg Glu Trp Val Ser Met Met Lys Val1 5 10 15Leu Cys 19 amino acids amino acid <Unknown> linear peptide not provided 47Ser Val Gln Cys Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Asn1 5 10 15His Leu Ser 19 amino acids amino acid <Unknown> linear peptide not provided 48Gly Asn Ala Asp Gly Pro Thr Leu Arg Gln Trp Leu Glu Gly Arg Arg1 5 10 15Pro Lys Asn 19 amino acids amino acid <Unknown> linear peptide not provided 49Ser Val Arg Cys Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Thr1 5 10 15His Leu Ser 19 amino acids amino acid <Unknown> linear peptide not provided 50Leu Ala Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu His Gly Asn Gly1 5 10 15Arg Asp Thr 19 amino acids amino acid <Unknown> linear peptide not provided 51His Gly Arg Val Gly Pro Thr Leu Arg Glu Trp Lys Thr Gln Val Ala1 5 10 15Thr Lys Lys 14 amino acids amino acid <Unknown> linear peptide not provided 52Cys Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 18 amino acids amino acid <Unknown> linear peptide not provided 53Ile Ser Asp Gly Pro Thr Leu Lys Glu Trp Leu Ser Val Thr Arg Gly1 5 10 15Ala Ser 18 amino acids amino acid <Unknown> linear peptide not provided 54Ser Ile Glu Gly Pro Thr Leu Arg Glu Trp Leu Thr Ser Arg Thr Pro1 5 10 15His Ser 18 amino acids amino acid <Unknown> linear peptide not provided 55Thr Ile Lys Gly Pro Thr Leu Arg Gln Trp Leu Lys Ser Arg Glu His1 5 10 15Thr Ser 19 amino acids amino acid <Unknown> linear peptide not provided 56Gly Asn Ala Asp Gly Pro Thr Leu Arg Gln Trp Leu Glu Gly Arg Arg1 5 10 15Pro Lys Asn 18 amino acids amino acid <Unknown> linear peptide not provided 57Ser Ile Glu Gly Pro Thr Leu Arg Glu Trp Leu Thr Ser Arg Thr Pro1 5 10 15His Ser 18 amino acids amino acid <Unknown> linear peptide not provided 58Ile Ser Asp Gly Pro Thr Leu Lys Glu Trp Leu Ser Val Thr Arg Gly1 5 10 15Ala Ser 10 amino acids amino acid <Unknown> linear peptide not provided 59Cys Ser Leu Glu Asp Leu Arg Lys Arg Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 60Cys Arg Arg Ser Glu Leu Leu Glu Arg Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 61Cys Thr Phe Lys Gln Phe Leu Asp Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 62Cys Thr Arg Gly Glu Trp Leu Arg Cys Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 63Cys Thr Leu Arg Gln Trp Leu Gln Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 64Cys Thr Leu Glu Glu Leu Arg Ala Cys Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 65Cys Thr Arg Glu Glu Leu Met Arg Leu Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 66Cys Gln Arg Ala Asp Leu Ile Asn Phe Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 67Cys Asn Arg Asn Asp Leu Leu Leu Phe Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 68Cys Thr Arg Thr Glu Trp Leu His Gly Cys1 5 10 9 amino acids amino acid <Unknown> linear peptide not provided 69Cys Thr Leu Glu Phe Met Asn Gly Cys1 5 10 amino acids amino acid <Unknown> linear peptide not provided 70Cys Ser Leu Gly Glu Leu Arg Arg Leu Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 71Cys Asn Ile Asn Gln Leu Arg Ser Ile Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 72Cys Thr Met Arg Gln Phe Leu Val Cys Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 73Cys Thr Arg Ser Glu Trp Leu Glu Arg Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 74Cys Thr Leu His Glu Tyr Leu Ser Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 75Cys Thr Arg Glu Glu Leu Leu Arg Gln Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 76Cys Thr Phe Arg Glu Phe Val Asn Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 77Cys Ser Arg Ala Asp Phe Leu Ala Ala Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 78Cys Ser Cys Ala Gln Val Val Gln Cys Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 79Cys Thr Leu Arg Gln Trp Ile Leu Leu Gly Met Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 80Cys Thr Leu Arg Glu Trp Leu His Gly Gly Phe Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 81Cys Thr Leu Arg Ala Trp Leu Met Ser Glu Thr Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 82Cys Thr Leu Arg Ala Trp Leu Met Glu Ser Cys Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 83Cys Thr Phe Gln Val Trp Lys Leu Ala Arg Asn Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 84Cys Leu Leu Arg Glu Trp Leu Asp Xaa Arg Thr Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 85Cys Val Leu Arg Glu Trp Leu Leu Xaa Xaa Ser Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 86Cys Leu Leu Ser Glu Phe Leu Ala Gly Gln Gln Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 87Cys Ser Leu Arg Gln Tyr Leu Asp Phe Gly Leu Gly Ser Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 88Cys Thr Leu Gln Glu Leu Lys Gln Ser Ser Leu Tyr Glu Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 89Cys Asp Leu Ser Glu Leu Lys Thr His Gly Tyr Ala Tyr Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 90Cys Lys Leu Ser Asp Trp Leu Met Asn Gly Val Ala Ala Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 91Cys Ser Leu Gln Glu Phe Leu Ser His Gly Gly Tyr Val Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 92Cys Ser Leu Lys Glu Phe Leu His Ser Gly Leu Met Gln Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 93Cys Thr Phe Arg Gln Leu Leu Glu Tyr Gly Val Ser Ser Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 94Cys Thr Met Arg Glu Phe Leu Val Ala Ser Gly Val Ala Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 95Cys Thr Leu Ala Glu Phe Leu Ala Ser Gly Val Glu Gln Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 96Cys Thr Leu Ala Glu Phe Leu Ala Ser Gly Val Glu Gln Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 97Cys Thr Leu Lys Glu Trp Leu Val Ser His Glu Val Trp Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 98Cys Thr Leu Arg Glu Phe Leu Ser Leu Gly Met Asn Ala Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 99Cys Thr Leu Arg Glu Phe Leu Asp Pro Thr Thr Ala Val Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 100Cys Ser Leu Leu Glu Phe Leu Ala Leu Gly Val Ala Leu Cys1 5 10 19 amino acids amino acid <Unknown> linear peptide not provided 101Gly Gly Gly Arg Gly Cys Thr Leu Lys Gln Trp Lys Gln Gly Asp Cy1 5 10 15Gly Arg Ser 10 amino acids amino acid <Unknown> linear peptide not provided 102Cys Asn Arg Ser Gln Leu Leu Ala Ala Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 103Cys Thr Leu Gln Gln Trp Leu Ser Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 104Cys Thr Leu Arg Glu Phe Lys Ala Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 105Cys Thr Arg Ala Gln Phe Leu Lys Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 106Cys Thr Leu Arg Glu Phe Asn Arg Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 107Cys Thr Leu Ser Asp Phe Lys Arg Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 108Cys Thr Phe Arg Gln Trp Lys Glu Ala Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 109Cys Thr Leu Ser Glu Phe Arg Gly Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 110Cys Thr Leu Gln Glu Phe Leu Glu Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 111Cys Thr Leu Gln Gln Trp Lys Asp Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 112Cys Thr Arg Ser Gln Trp Leu Glu Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 113Cys Ser Leu Gln Glu Phe Lys His Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 114Cys Thr Leu Gly Glu Trp Lys Arg Gly Cys1 5 10 11 amino acids amino acid <Unknown> linear peptide not provided 115Cys Thr Leu Trp Gly Cys Gly Lys Arg Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 116Cys Thr Leu Gln Glu Trp Arg Gly Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 117Cys Thr Arg Leu Ser Gly Cys Trp Leu Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 118Cys Thr Arg Thr Gln Trp Leu Leu Asp Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 119Cys Thr Leu Ala Glu Phe Arg Arg Gly Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 120Cys Thr Ser Thr Gln Trp Leu Leu Ala Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 121Cys Ser Arg Ser Gln Phe Leu Arg Ser Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 122Cys Thr Leu Arg Glu Trp Leu Glu Gly Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 123Cys Thr Leu Arg Glu Phe Leu Leu Met Gly Ala Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 124Cys Thr Leu Lys Glu Trp Leu Leu Trp Ser Ser Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 125Cys Thr Leu Leu Glu Trp Leu Arg Asn Pro Val Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 126Cys Thr Leu Arg Gln Trp Leu Gly Asp Ala Trp Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 127Cys Thr Leu Gly Gln Trp Leu Gln Met Gly Met Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 128Cys Thr Leu Arg Glu Trp Val Phe Ala Gly Leu Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 129Cys Leu Leu Leu Glu Phe Leu Ser Gly Ala Asp Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 130Cys Thr Leu Gly Glu Phe Leu Ala Gly His Leu Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 131Cys Arg Leu Arg Glu Phe Leu Val Asp Leu Thr Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 132Cys Ser Phe Arg Ser Trp Leu Val Asp Gln Thr Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 133Cys Thr Leu Arg Glu Trp Leu Glu Asp Leu Gly Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 134Cys Thr Leu Gln Asp Trp Leu Val Ser Trp Thr Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 135Cys Thr Leu Ser Glu Trp Leu Ser Glu Leu Ser Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 136Cys Thr Leu Met Gln Trp Leu Gly Gly Trp Pro Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 137Cys Thr Leu Arg Glu Trp Leu Ser Tyr Gly Thr Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 138Cys Thr Leu Gln Glu Trp Leu Ser Gly Gly Leu Cys1 5 10 18 amino acids amino acid <Unknown> linear peptide not provided 139Gly Ser His Gly Cys Thr Leu Arg Glu Trp Leu Cys Met Lys Ile Val1 5 10 15Pro Cys 18 amino acids amino acid <Unknown> linear peptide not provided 140Gln Trp Gln Gly Cys Thr Leu Arg Asp Cys Ile Leu Arg Gly Val Phe1 5 10 15Trp Ser 18 amino acids amino acid <Unknown> linear peptide not provided 141Ser Val Asn Ser Cys Thr Leu Arg Glu Phe Leu Thr Gly Cys Arg Val1 5 10 15Phe Cys 18 amino acids amino acid <Unknown> linear peptide not provided 142Ser Tyr Asp Gly Cys Thr Leu Arg His Trp Leu Met Asp Ile Tyr Gly1 5 10 15Asp Cys 18 amino acids amino acid <Unknown> linear peptide not provided 143Gln Arg Ser Gly Cys Thr Leu Arg Asp Trp Val Leu Leu Asn Cys Leu1 5 10 15Ala Ser 18 amino acids amino acid <Unknown> linear peptide not provided 144Asn Tyr Arg Gly Cys Thr Leu Ser Gln Trp Val Ser Glu Gln Ile Val1 5 10 15Gly Cys 18 amino acids amino acid <Unknown> linear peptide not provided 145Gly Arg Ser Gly Cys Thr Leu Arg Glu Tyr Leu Gly Gly Met Cys Tyr1 5 10 15Leu Ser 18 amino acids amino acid <Unknown> linear peptide not provided 146Ala Ser Trp Tyr Cys Thr Val Pro Glu Leu Met Glu Met Gln Leu Pro1 5 10 15Glu Cys 18 amino acids amino acid <Unknown> linear peptide not provided 147Gly Ser Thr Gly Cys Thr Leu Arg Glu Xaa Leu His Met Leu Gly Leu1 5 10 15Asp Cys 18 amino acids amino acid <Unknown> linear peptide not provided 148Ala Cys Glu Gly Cys Thr Leu Arg Gln Trp Leu Glu Tyr Val Arg Val1 5 10 15Gly Cys 18 amino acids amino acid <Unknown> linear peptide not provided 149Ala Gln Arg Gly Cys Thr Leu Gln Tyr Phe Val Ser Tyr Gly Xaa Asp1 5 10 15Met Cys 18 amino acids amino acid <Unknown> linear peptide not provided 150Gly Val Cys Gly Cys Thr Leu Arg Glu Phe Leu Ala Ile Pro His Thr1 5 10 15Ser Cys 18 amino acids amino acid <Unknown> linear peptide not provided 151Ser Glu Gly Gly Cys Thr Leu Arg Glu Trp Val Ala Ser Ser Leu Ala1 5 10 15Asn Cys 18 amino acids amino acid <Unknown> linear peptide not provided 152Ser Asn Ser Arg Cys Thr Leu Arg Glu Trp Ile Ile Gln Gly Cys Asp1 5 10 15Phe Ser 18 amino acids amino acid <Unknown> linear peptide not provided 153Ser Asn Ser Arg Cys Thr Leu Arg Glu Trp Ile Ile Gln Gly Cys Asp1 5 10 15Phe Ser 18 amino acids amino acid <Unknown> linear peptide not provided 154Cys Leu Gly Cys Thr Leu Ser Gln Trp Arg Lys Arg Thr Arg Cys Asp1 5 10 15Thr His 17 amino acids amino acid <Unknown> linear peptide not provided 155Tyr Arg Gly Cys Ser Arg Ala Gln Leu Leu Gly Gly Glu Cys Arg Lys1 5 10 15Lys 17 amino acids amino acid <Unknown> linear peptide not provided 156Gly Arg Gly Cys Thr Leu Lys Gln Trp Lys Gln Gly Asp Cys Gly Arg1 5 10 15Ser 19 amino acids amino acid <Unknown> linear peptide not provided 157Val Arg Gly Gly Cys Ala Leu Arg Asp Trp Val Ala Gly Glu Cys Phe1 5 10 15Asp Trp Thr 19 amino acids amino acid <Unknown> linear peptide not provided 158Leu Trp Arg Gly Cys Thr Leu Asn Gly Phe Lys Ser Arg His Cys Gly1 5 10 15Ser Pro Glu 13 amino acids amino acid <Unknown> linear peptide not provided 159Cys Thr Leu Arg Ser Trp Lys His Arg Gly Cys Ala Pro1 5 10 17 amino acids amino acid <Unknown> linear peptide not provided 160Gly Arg Gly Cys Thr Arg Ala Gln Trp Leu Ala Gly Cys Cys Thr Gly1 5 10 15His 16 amino acids amino acid <Unknown> linear peptide not provided 161Arg Ala Gly Cys Thr Leu Arg Glu Phe Arg Lys Gly Cys Leu Ala Leu1 5 10 15 17 amino acids amino acid <Unknown> linear peptide not provided 162Lys Arg Gly Cys Thr Leu Ala Glu Met Ile Arg Gly Cys Asn Arg Ser1 5 10 15Asn 17 amino acids amino acid <Unknown> linear peptide not provided 163Gly Arg Gly Cys Thr Leu Lys Gln Trp Lys Gln Gly Asp Cys Gly Arg1 5 10 15Ser 19 amino acids amino acid <Unknown> linear peptide not provided 164Arg Trp Arg Gly Cys Ser Leu Ala Lys Leu Lys Lys Gly Ala Ala Cys1 5 10 15Gly Arg Gly 16 amino acids amino acid <Unknown> linear peptide not provided 165Arg Gly Gly Cys Thr Leu Arg Glu Trp Arg Arg Val Arg Val Ile Asn1 5 10 15 17 amino acids amino acid <Unknown> linear peptide not provided 166Gly Arg Gly Cys Thr Leu Lys Gln Trp Lys Gln Gly Asp Cys Gly Arg1 5 10 15Ser 17 amino acids amino acid <Unknown> linear peptide not provided 167Arg Tyr Gly Cys Thr Arg His Gln Trp Leu Val Gly Thr Cys Val Arg1 5 10 15His 8 amino acids amino acid <Unknown> linear peptide not provided 168Gly Pro Thr Leu Arg Gln Trp Leu1 5 11 base pairs nucleic acid single linear other nucleic acid /desc = “oligonucleotide” not provided 169ACCACCTCCG G 11 11 base pairs nucleic acid single linear other nucleic acid /desc = “oligonucleotide” not provided 170TTACTTAGTT A 11 25 base pairs nucleic acid single linear other nucleic acid /desc = “oligonucleotide” not provided 171GAGGTGGTNN KTAACTAAGT AAAGC 25 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “Pen” 172Xaa Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 13 amino acids amino acid <Unknown> linear peptide not provided 173Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “Homocys” 174Xaa Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “D-Cys” Modified-site 14 /product= “D-Cys” 175Xaa Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Xaa1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site 14 /product= “D-Cys” 176Cys Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Xaa1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “D-Pen” Modified-site 14 /product= “D-Cys” 177Xaa Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Xaa1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “Homocys” Modified-site 14 /product= “Homocys” 178Xaa Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Xaa1 5 10 13 amino acids amino acid <Unknown> linear peptide not provided Modified-site 13 /product= “Homocys” 179Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Xaa1 5 10 13 amino acids amino acid <Unknown> linear peptide not provided Modified-site 13 /product= “Pen” 180Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Xaa1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 181Lys Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Glu1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 182Glu Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Lys1 5 10 13 amino acids amino acid <Unknown> linear peptide not provided 183Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Glu1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “Pen” Modified-site 14 /product= “Pen” 184Xaa Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Xaa1 5 10 15 amino acids amino acid <Unknown> linear peptide not provided 185Cys Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Ala1 5 10 15 13 amino acids amino acid <Unknown> linear peptide not provided 186Cys Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe1 5 10 19 amino acids amino acid <Unknown> linear peptide not provided 187Ser Val Gln Cys Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Asn1 5 10 15His Leu Ser 10 amino acids amino acid <Unknown> linear peptide not provided 188Met Val Gly Pro Thr Leu Arg Ser Gly Cys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 189Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 11 amino acids amino acid <Unknown> linear peptide not provided 190Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 10 amino acids amino acid <Unknown> linear peptide not provided 191Pro Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 10 9 amino acids amino acid <Unknown> linear peptide not provided 192Thr Leu Arg Glu Trp Ile Ser Phe Cys1 5 14 amino acids amino acid <Unknown> linear peptide not provided 193Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Ala1 5 10 16 amino acids amino acid <Unknown> linear peptide not provided 194Cys Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Ala Cys1 5 10 15 14 amino acids amino acid <Unknown> linear peptide not provided 195Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Lys1 5 10 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site 14 /product= “Beta-ala” 196Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Xaa Lys1 5 10 15 13 amino acids amino acid <Unknown> linear peptide not provided 197Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Lys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site 13 /product= “Beta-ala” 198Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Xaa Lys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided 199Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Ala Lys1 5 10 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site 14 /product= “Beta-ala” 200Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Ala Xaa Lys1 5 10 15 13 amino acids amino acid <Unknown> linear peptide not provided 201Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Lys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site 13 /product= “Beta-ala” 202Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Xaa Lys1 5 10 12 amino acids amino acid <Unknown> linear peptide not provided 203Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Lys1 5 10 13 amino acids amino acid <Unknown> linear peptide not provided Modified-site 12 /product= “Beta-ala” 204Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Xaa Lys1 5 10 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “Sar” Modified-site /product= “1-Nal” 205Ile Glu Xaa Pro Thr Leu Arg Gln Xaa Leu Ala Ala Arg Ala Lys1 5 10 15 16 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “Sar” Modified-site /product= “1-Nal” Modified-site 15 /product= “Beta-ala” 206Ile Glu Xaa Pro Thr Leu Arg Gln Xaa Leu Ala Ala Arg Ala Xaa Lys1 5 10 15 13 amino acids amino acid <Unknown> linear peptide not provided Modified-site 11 /product= “Ava” 207Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Xaa Arg Lys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site 11 /product= “Ava” Modified-site 13 /product= “Beta-ala” 208Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Xaa Arg Xaa Lys1 5 10 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site 14 /product= “N-methyl-Ala” 209Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Xaa Lys1 5 10 15 16 amino acids amino acid <Unknown> linear peptide not provided Modified-site 14 /product= “N-methyl-Ala” Modified-site 15 /product= “Beta-ala” 210Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Xaa Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site 13 /product= “p-amino-Phe” 211Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Xaa Ala Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site 13 /product= “Ac-Lys” 212Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Xaa Ala Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “Ac-Lys” Modified-site 13 /product= “Ac-Lys” 213Ile Glu Gly Pro Thr Leu Xaa Gln Trp Leu Ala Ala Xaa Ala Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “1-Nal” Modified-site 14 /product= “Beta-ala” 214Ile Glu Gly Pro Thr Leu Arg Gln Xaa Leu Ala Ala Arg Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site 14 /product= “Sar” 215Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “1-Nal” Modified-site 14 /product= “Sar” 216Ile Glu Gly Pro Thr Leu Arg Gln Xaa Leu Ala Ala Arg Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site 14 /product= “Beta-ala” 217Ile Glu Gly Pro Thr Leu Arg Gln Phe Leu Ala Ala Arg Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “1-Nal” Modified-site 13 /product= “Ac-Lys” Modified-site 14 /product= “Sar” 218Ile Glu Gly Pro Thr Leu Arg Gln Xaa Leu Ala Ala Xaa Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “1-Nal” Modified-site 13 /product= “Ac-Lys” Modified-site 14 /product= “Sar” 219Ile Glu Gly Pro Thr Leu Arg Glu Xaa Leu Ala Ala Xaa Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “1-Nal” Modified-site 13 /product= “Ac-Lys” Modified-site 14 /product= “Sar” 220Ile Glu Gly Pro Thr Leu Ala Gln Xaa Leu Ala Ala Xaa Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “1-Nal” Modified-site 13 /product= “Ac-Lys” Modified-site 14 /product= “Sar” 221Ile Glu Gly Pro Thr Leu Ala Glu Xaa Leu Ala Ala Xaa Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “Nal” Modified-site 13 /product= “Nle” Modified-site 14 /product= “Sar” 222Ile Glu Gly Pro Thr Leu Arg Gln Xaa Leu Ala Ala Xaa Xaa Lys1 5 10 15 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site /product= “Nle” Modified-site /product= “1-Nal” Modified-site 13 /product= “Nle” Modified-site 14 /product= “Sar” 223Ile Glu Gly Pro Thr Leu Xaa Gln Xaa Leu Ala Ala Xaa Xaa Lys1 5 10 15 13 amino acids amino acid <Unknown> linear peptide not provided Modified-site 11 /product= “Abu” Modified-site 12 /product= “DipheAla” 224Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Xaa Xaa Lys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site 11 /product= “Abu” Modified-site 12 /product= “Diphe” Modified-site 13 /product= “Beta-ala” 225Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Xaa Xaa Xaa Lys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site 11 /product= “Abu” Modified-site 12 /product= “DipheAla” 226Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Xaa Xaa Arg Lys1 5 10 15 amino acids amino acid <Unknown> linear peptide not provided Modified-site 11 /product= “Abu” Modified-site 12 /product= “Diphe” Modified-site 14 /product= “Beta-ala” 227Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Xaa Xaa Arg Xaa Leu1 5 10 15 13 amino acids amino acid <Unknown> linear peptide not provided Modified-site 11 /product= “Abu” Modified-site 12 /product= “DipheAla” 228Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Xaa Xaa Lys1 5 10 14 amino acids amino acid <Unknown> linear peptide not provided Modified-site 11 /product= “Abu” Modified-site 12 /product= “DipheAla” Modified-site 13 /product= “Beta-ala” 229Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Xaa Xaa Xaa Lys1 5 10 13 amino acids amino acid <Unknown> linear peptide not provided Modified-site 11 /product= “Abu” Modified-site 12 /product= “DipheAla” 230Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Xaa Xaa Cys1 5 10 25 amino acids amino acid <Unknown> linear peptide not provided Modified-site 13 /product= “Ava” 231Ala Asp Gly Pro Thr Leu Arg Glu Trp Ile Ser Phe Xaa Ala Asp Gly1 5 10 15Pro Thr Leu Arg Glu Trp Ile Ser Phe 20 25 16 amino acids amino acid <Unknown> linear peptide not provided 232Cys Ile Glu Gly Pro Thr Leu Arg Gln Trp Leu Ala Ala Arg Ala Lys1 5 10 15 93 base pairs nucleic acid double linear cDNA not provided CDS 1..84 233CTC GAG AGC GGG CAG GTG GTG CAT GGG GAG CAG GTG GGT GGT GAG GCC 48Leu Glu Ser Gly Gln Val Val His Gly Glu Gln Val Gly Gly Glu Ala 1 5 10 15TCC GGG GCC GTT AAC GGC CGT GGC CTA GCT GGC CAA TAAGTCGAC 93Ser Gly Ala Val Asn Gly Arg Gly Leu Ala Gly Gln 20 25 28 amino acids amino acid linear protein not provided 234Leu Glu Ser Gly Gln Val Val His Gly Glu Gln Val Gly Gly Glu Ala 1 5 10 15Ser Gly Ala Val Asn Gly Arg Gly Leu Ala Gly Gln 20 25 92 base pairs nucleic acid double linear cDNA not provided CDS 1..63 235CTC GAG AGC GGG CAG GTG GTG CAT GGG GAG CAG GTG GGT GGT GAG GCC 48Leu Glu Ser Gly Gln Val Val His Gly Glu Gln Val Gly Gly Glu Ala 1 5 10 15TCC GGA GGT GGT NNK TAACTAAGTA AAGCTGGCCA ATAAGTCGA 92Ser Gly Gly Gly Xaa 20 21 amino acids amino acid linear protein not provided 236Leu Glu Ser Gly Gln Val Val His Gly Glu Gln Val Gly Gly Glu Ala 1 5 10 15Ser Gly Gly Gly Xaa 20 122 base pairs nucleic acid double linear cDNA not provided CDS 1..120 237CAG ACT AAT TCG AGC TCG AAC AAC AAC AAC AAT AAC AAT AAC AAC AAC 48Gln Thr Asn Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn 1 5 10 15CTC GGG ATC GAG GGA AGG ACC GGT CAC GTG GCC CGG GAA TTC GGA TCC 96Leu Gly Ile Glu Gly Arg Thr Gly His Val Ala Arg Glu Phe Gly Ser 20 25 30TCT AGA GTC GAC CTG CAG GCA AGC TT 122Ser Arg Val Asp Leu Gln Ala Ser 35 40 40 amino acids amino acid linear protein not provided 238Gln Thr Asn Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn 1 5 10 15Leu Gly Ile Glu Gly Arg Thr Gly His Val Ala Arg Glu Phe Gly Ser 20 25 30Ser Arg Val Asp Leu Gln Ala Ser 35 40 51 base pairs nucleic acid double linear cDNA not provided CDS 1..21 239GGA AGG ACC GGA GGT GGT NNK TAACTAAGTA AAGCTGGCCA ATAAGTCGAC 51Gly Arg Thr Gly Gly Gly Xaa 1 5 7 amino acids amino acid linear protein not provided 240Gly Arg Thr Gly Gly Gly Xaa 1 5 93 base pairs nucleic acid double linear cDNA not provided CDS 1..84 241GAA GCG GCG ATG GCG GAG CTG AAT TAC ATT CCC CGG TCG CAG GAG GCC 48Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile Pro Arg Ser Gln Glu Ala 1 5 10 15TCC GGG GCC GTT AAC GGC CGT GGC CTA GCT GGC CAA TAAGTCGAC 93Ser Gly Ala Val Asn Gly Arg Gly Leu Ala Gly Gln 20 25 28 amino acids amino acid linear protein not provided 242Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile Pro Arg Ser Gln Glu Ala 1 5 10 15Ser Gly Ala Val Asn Gly Arg Gly Leu Ala Gly Gln 20 25 126 base pairs nucleic acid double linear cDNA not provided CDS 1..96 243GAA GCG GCG ATG GCG GAG CTG AAT TAC ATT CCC CGG TCG CAG GAG GCC 48Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile Pro Arg Ser Gln Glu Ala 1 5 10 15TCC GGA GGT GGT NNK NNK NNK NNK NNK NNK NNK NNK NNK NNK NNK NNK 96Ser Gly Gly Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30TAACTAAGTA AAGCTGGCCA ATAAGTCGAC 126 32 amino acids amino acid linear protein not provided 244Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile Pro Arg Ser Gln Glu Ala 1 5 10 15Ser Gly Gly Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30

Claims

1. A method of activating a thrombopoietin receptor in a cell, comprising contacting said cell with an effective amount of a compound covalently attached to a hydrophilic polymer, said compound selected from the group consisting of:

2. The method of claim 1 wherein said hydrophilic polymer has an average molecular weight of between about 500 to about 40,000 daltons.

3. The method of claim 1 wherein said hydrophilic polymer has an average molecular weight of between about 5,000 to about 20,000 daltons.

4. The method claim 1 wherein said polymer is selected from the group consisting of polyethylene glycol, polypropylene glycol, polylactic acid and polyglycolic acid.

5. The method of claim 4 wherein said compound is covalently attached to polyethylene glycol.

6. The method of claim 1 wherein each of the dimeric subunits of said compound is covalently attached to a hydrophilic polymer.

7. A method according to claim 1 wherein said cells are in vivo.

8. A method according to claim 1 wherein said cells are in vitro.

9. A method according to claim 1 wherein said cells comprise human megakaryocytes, platelets or CD34+ cells.

10. A method according to claim 1 wherein said cells comprise TPO-dependent cells.

11. A method of treating thrombocytopenia in a subject, comprising:(a) obtaining a population of said subject's cells comprising megakaryocyte precursor cells; (b) treating said cells according to the method of claim 1; and (c) administering said treated cells to said subject, to increase the number of megakaryocytes present in said subject compared to that which would occur without such treatment.

12. A method according to claim 11 wherein said thrombocytopenia is due to chemotherapy.

13. A method according to claim 12 where said population of cells is obtained prior to said chemotherapy.

14. A method according to claim 11 wherein said thrombocytopenia is due to radiation therapy.

15. A method according to claim 14 where said population of cells is obtained prior to said radiation therapy.

16. A method of treating a patient suffering from thrombocytopenia, comprising administering to said patient a therapeutically effective dose of a compound covalently attached to a hydrophilic polymer, said compound selected from the group consisting of:

17. A method according to claim 16, wherein said hydrophilic polymer has an average molecular weight of between about 500 to about 40,000 daltons.

18. A method according to claim 16, wherein said hydrophilic polymer has an average molecular weight of between about 5,000 to about 20,000 daltons.

19. The method of claim 16, wherein said polymer is selected from the group consisting of polyethylene glycol, polypropylene glycol, polylactic acid and polyglycolic acid.

20. The method of claim 16 wherein said compound is covalently attached to polyethylene glycol.

21. The method of claim 16 wherein each of the dimeric subunits of said compound is covalently attached to a hydrophilic polymer.

22. A method according to claim 16 wherein said thrombocytopenia is due to chemotherapy or radiation therapy.

23. A method according to claim 22 wherein a TPO antagonist is administered to said patient prior to said chemotherapy or radiation therapy.

24. A method according to claim 26 wherein said thrombocytopenia is due to bone marrow transfusion.

25. A method of prophylactically treating a patient at risk of thrombocytopenia, comprising administering to said patient a prophylactically effective amount of a compound covalently attached to a hydrophilic polymer, said compound selected from:

26. A method according to claim 25 where said compound is administered prior to bone marrow transplantation, chemotherapy, or radiation therapy.

27. A method according to claim 25, wherein said hydrophilic polymer has an average molecular weight of between about 500 to about 40,000 daltons.

28. A method according to claim 25, wherein said hydrophilic polymer has an average molecular weight of between about 5,000 to about 20,000 daltons.

29. The method of claim 25, wherein said polymer is selected from the group consisting of polyethylene glycol, polypropylene glycol, polylactic acid and polyglycolic acid.

30. The method of claim 25 wherein said compound is covalently attached to polyethylene glycol.

31. The method of claim 25 wherein each of the dimeric subunits of said compound is covalently attached to a hydrophilic polymer.