Patent Grant
9439942
The ASCII file, entitled 60100SequenceListing.txt, created on Aug. 4, 2014, comprising 45,056 bytes, submitted concurrently with the filing of this application is incorporated herein by reference.
The present invention, in some embodiments thereof, relates to peptides and use thereof in the treatment of large cell lung cancer.
Despite advances in surgery, chemotherapy and radiotherapy over the last decades, the death rate from lung cancer remains a global health problem and worldwide, lung cancer is the most common cause of cancer-related death in men and women. The two main types of lung cancer are small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC), NSCLC being any type of epithelial lung cancer other than small cell lung carcinoma (SCLC). NSCLC accounts for approximately 85% of all lung cancers. NSCLC is typically divided into adenocarcinoma, squamous cell carcinoma (SCC), and large cell carcinoma histologies, with approximately 10-15% of all NSCLC being large cell lung cancer. Of the non-small cell lung cancers, large cell lung cancer is usually discovered at a later stage. Furthermore, large cell lung cancers tend to be more aggressive and metastasize. The tumor metastasizes into nearby lymph nodes, into the chest wall and into more distant organs, even when the tumor in the lung is relatively small. As diagnosis occurs at an advanced stage, prognosis is typically poor. Large cell lung cancer is insensitive to chemotherapy compared to small cell lung carcinoma. Thus, treatment of NSCLC typically requires multiple treatments including surgical resection, chemotherapeutic agents and/or radiation therapy used both pre-operatively (neoadjuvant chemotherapy) and post-operatively (adjuvant chemotherapy). Among the most widely used chemotherapeutic drugs are cisplatin and paclitaxel.
The chemokine receptor CXCR4 is a G-protein coupled receptor that is expressed in a wide assortment of normal tissues, and plays a fundamental role in fetal development, mobilization of hematopoietic stem cells and trafficking of naive lymphocytes (Rossi and Zlotnik, 2000). The chemokine CXCL12 (also known as stromal-derived factor-1 or SDF-1) is the only natural ligand of CXCR4. CXCL12 is expressed constitutively in a variety of tissues, including lung, liver, bone marrow and lymph nodes.
Binding of CXCL12 to CXCR4 activates a variety of intracellular signal transduction pathways and effector molecules that regulate cell chemotaxis, adhesion, survival and proliferation. For example, the phosphatidyl-inositol-3-kinase pathway and the mitogen-activated protein (MAP) kinase pathways are regulated by CXCL12 and CXCR4.
Various uses of chemokine receptor modulators, including CXCR4 agonists and antagonists, have been described in the art (Princen et al., 2005; Tamamura et al., 2005; U.S. Pat. No. 7,169,750). The bicyclam drug termed AMD3100, originally discovered as an anti-HIV compound, specifically interacts with CXCR4 in an antagonistic manner. Blocking CXCR4 receptor with AMD3100 results in the mobilization of hematopoietic progenitor cells. Other compounds having CXCR4 regulating activity have been described in the art such as in U.S. Publication No. 2007/0167459, U.S. Pat. No. 6,946,445, and U.S. Patent Application Publication No. 2005/0002939. Moreover, various therapeutic applications have been suggested, such as in cancer therapy. T-140 is a 14-residue synthetic peptide developed as a specific CXCR4 antagonist that suppress HIV-1 (X4-HIV-1) entry to T cells through specific binding to CXCR4 (Tamamura et al., 1998). Subsequently, peptide analogs of T-140 were developed as specific CXCR4 antagonist peptides with inhibitory activity at nanomolar levels (see Tamamura et al., 2003, WO 2002/020561 and WO 2004/020462, WO 2004/087068, WO 00/09152, US 2002/0156034, and WO 2004/024178).
WO 2004/087068 discloses antagonists of chemokine receptors, particularly the CXCR4 receptor, and methods of their use, for example, in the treatment, prevention or diagnosis of cancer. The '068 publication discloses that exemplary CXCR4 peptide antagonists include T140 and derivatives of T140, and that the pathology includes cancer such as breast, brain, pancreatic, ovarian, prostate, kidney, and non-small lung cancer.
WO 00/09152 discloses a variety of therapeutic uses for CXCR4 antagonists such as in the treatment of cancer.
WO 2004/024178 discloses the use of a chemokine receptor antagonist as a ligand for the CXCR4 receptor for the apoptosis-inducing treatment and/or the prevention of the metastatic spread of cancer cells in a patient.
U.S. Publication No. 2002/0156034 discloses the use of CXCR4 antagonists for the treatment of hematopoietic cells such as in cancer.
WO 2002/020561 discloses peptide analogs and derivatives of T-140. The '561 publication demonstrates that the claimed peptides are potent CXCR4 inhibitors, manifesting high anti-HIV virus activity and low cytotoxicity.
WO 2004/020462 discloses additional novel peptide analogs and derivatives of T-140, including 4F-benzoyl-TN14003. The '462 publication further discloses preventive and therapeutic compositions and methods of using same utilizing T-140 analogs for the treatment of cancer, such as lung cancer.
Various therapeutic applications for 4F-benzoyl-TN14003 analogs and derivatives have been recently suggested. Some are described in the following related art.
WO 08/075369 is directed to therapeutic uses of T-140 analog peptides and compositions comprising same. Particularly, the '369 publication provides compositions and methods for providing improved bone marrow transplantation and in the treatment of other conditions wherein bone marrow depletion or suppression is involved.
WO 08/075370 is directed to novel therapeutic uses of T-140 analog peptides, compositions comprising same, and use thereof useful in cancer therapy.
WO 08/075371 is directed to novel therapeutic uses of T-140 analog peptides, compositions comprising same, and use thereof useful for immunomodulation.
WO 10/146578 provides compositions comprising T-140 analog peptides and methods of use thereof, specifically for providing improved platelet levels useful in the treatment and prevention of thrombocytopenia, for controlling bleeding and for inducing or modulating haemostasis.
WO 10/146584 discloses novel polypeptides comprising a chemokine-binding peptide and an Fc fragment. According to the '584 publication the polypeptides are capable of binding to certain chemokines so as to modulate their activity, and are therefore useful in modulating in vivo chemokine-dependent processes such as inflammation, autoimmunity and cancer.
Avniel et al. (Avniel et al., 2006) discloses that blocking the CXCR4/CXCL12 axis by a T-140 analog resulted in a significant reduction in eosinophil accumulation in the dermis and improved epithelialization, thus significantly improving skin recovery after burns.
According to an aspect of some embodiments of the present invention there is provided a method of treating large cell lung cancer in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a peptide comprising an amino acid sequence as set forth in SEQ ID NO:1 or an analog or derivative thereof, thereby treating the large cell lung cancer in the subject.
According to an aspect of some embodiments of the present invention there is provided a method of inducing death or inhibiting growth of tumor cells of large cell lung cancer, the method comprising contacting the tumor cells with a peptide comprising an amino acid sequence as set forth in SEQ ID NO:1 or an analog or derivative thereof, thereby inducing death or inhibiting growth of tumor cells of large cell lung cancer.
According to an aspect of some embodiments of the present invention there is provided a peptide comprising an amino acid sequence as set forth in SEQ ID NO:1 or an analog or derivative thereof for use in the treatment of large cell lung cancer.
According to some embodiments of the invention, the analog or derivative comprises an amino acid sequence as set forth in formula (I) or a salt thereof:
wherein:
According to some embodiments of the invention, the peptide is selected from the group consisting of SEQ ID NOS: 1-72.
According to some embodiments of the invention, the peptide is derivatized at the N terminus with a substituted benzoyl group.
According to some embodiments of the invention, the peptide is selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 36-37 and SEQ ID NO: 53-56.
According to some embodiments of the invention, the peptide consists of SEQ ID NO: 1.
According to some embodiments of the invention, the administering is effected intratumorally.
According to some embodiments of the invention, the method further comprises administering a chemotherapy agent to the subject.
According to some embodiments of the invention, the chemotherapy agent is an alkylating-like agent.
According to some embodiments of the invention, the alkylating-like agent is cisplatin.
According to some embodiments of the invention, the chemotherapy agent is a mitotic inhibitor.
According to some embodiments of the invention, the mitotic inhibitor comprises paclitaxel.
According to some embodiments of the invention, the method further comprises subjecting the subject to radiation therapy.
According to some embodiments of the invention, the peptide induces the tumor cell death.
According to some embodiments of the invention, the peptide inhibits the tumor growth.
According to some embodiments of the invention, the tumor cells comprise tumor stem cells.
Unless otherwise defined, all technical and/or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of embodiments of the invention, exemplary methods and/or materials are described below. In case of conflict, the patent specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be necessarily limiting.
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
Some embodiments of the invention are herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and for purposes of illustrative discussion of embodiments of the invention. In this regard, the description taken with the drawings makes apparent to those skilled in the art how embodiments of the invention may be practiced.
In the drawings:
The present invention, in some embodiments thereof, relates to peptides and uses of same in the treatment of large cell lung cancer.
The principles and operation of the present invention may be better understood with reference to the drawings and accompanying descriptions.
Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not necessarily limited in its application to the details set forth in the following description or exemplified by the Examples. The invention is capable of other embodiments or of being practiced or carried out in various ways. Also, it is to be understood that the phraseology and terminology employed herein is for the purpose of description and should not be regarded as limiting.
CXCR4/CXCL12 interactions promote non-small cell lung cancer (NSCLC) growth and dissemination. In addition, evidence suggests that this axis plays an important role in promoting lung cancer resistance to chemo/radio therapy.
While reducing the present invention to practice, the present inventors have uncovered a novel therapeutic use for the small CXCR4-antagonistic peptide in the treatment of NSCLC.
As is shown hereinbelow and in the Examples section which follows, the present inventors have demonstrated through laborious experimentation the therapeutic efficacy of BKT140 against human NSCLC. Specifically, the present inventors utilized five CXCR4 expressing NSCLC cell lines (see
Thus, according to an aspect of the invention there is provided a method of treating large cell lung cancer in a subject in need thereof. The method comprising administering to the subject a therapeutically effective amount of a peptide comprising an amino acid sequence as set forth in SEQ ID NO: 1 or an analog or derivative thereof, thereby treating the large cell lung cancer in the subject.
Alternatively or additionally, there is provided a method of inducing death or inhibiting growth of tumor cells of large cell lung cancer. The method comprising contacting (i.e., in-vitro, in-vivo or ex-vivo) the tumor cells with a peptide comprising an amino acid sequence as set forth in SEQ ID NO:1 or an analog or derivative thereof, thereby inducing death or inhibiting growth of tumor cells of large cell lung cancer.
Still alternatively or additionally, there is provided a peptide comprising an amino acid sequence as set forth in SEQ ID NO:1 or an analog or derivative thereof for use in the treatment of large cell lung cancer.
As used herein, the term “peptide” encompasses native peptides (either degradation products, synthetically synthesized peptides or recombinant peptides) and peptidomimetics (typically, synthetically synthesized peptides), as well as peptoids and semipeptoids which are peptide analogs, which may have, for example, modifications rendering the peptides more stable while in a body or more capable of penetrating into cells.
The peptides of the present invention are interchangeably referred to as , 4F-benzoyl-TN14003 (BKT140, SEQ ID NO: 1) analogs and derivatives and are structurally and functionally related to the peptides disclosed in patent applications WO 2002/020561 and WO 2004/020462, also known as “T-140 analogs”, as detailed hereinbelow. Without being bound by theory it is suggested that peptides of the invention induce growth arrest and/or death of cancer stem cells of the tumor.
In this specification and drawings, the representations of amino acids, etc. by brevity codes are made by the use of the codes prescribed by IUPAC-IUB Commission on Biochemical Nomenclature or by the codes customarily used in the relevant art. Examples of such codes are shown below. If an optical isomer exists with respect to an amino acid, it preferably represents the L form unless otherwise expressly specified.
Gly or G: glycine; Ala or A: alanine; Val or V: valine; Leu or L: leucine; Ile or I: isoleucine; Ser or S: serine; Thr or T: threonine; Cys or C: cysteine; Met or M: methionine; Glu or E: glutamic acid; Asp or D: aspartic acid; Lys or K: lysine; Arg or R: arginine; His or H: histidine; Phe or F: phenylalanine; Tyr or Y: tyrosine; Trp or W: tryptophan; Pro or P: proline; Asn or N: asparagine; Gln or Q: glutamine; pGlu: pyroglutamic acid; NaI: 3-(2-naphthyl) alanine; Cit: citrulline; DLys: D-lysine; DCit: D-citrulline; DG1u: D-glutamic acid; Me: methyl group; Et: ethyl group; Bu: butyl group; Ph: phenyl group.
The substituents, protective group and reagents often used in this specification are indicated by the following codes.
In N-terminal amino acids, [H—] indicates that the terminal amino group is not derivatized, and in C-terminal amino acids, [—OH] indicates that the terminal carboxyl group is not derivatized.
The 4F-benzoyl-TN14003 analogs of the invention belong to a family of structurally closely related peptides, also known as T-140 analogs. T-140 is a known synthetic peptide having the amino acid sequence H-Arg-Arg-Nal-Cys-Tyr-Arg-Lys-DLys-Pro-Tyr-Arg-Cit-Cys-Arg-OH (SEQ ID NO: 69, Tamamura et al., 2003, Supra), which was designed based on tachyplesin family polypeptides of the horseshoe crab. The preferable peptides of the invention include analogs and derivatives disclosed in patent applications WO 2002/020561 and WO 2004/020462. These peptides are synthetic peptides of artificial origin.
The term “analog” of SEQ ID NO: 1 as used herein thus relates to a peptide having at least 60% identity to SEQ ID NO: 1, preferably a peptide of Formulae (I) or (II) as defined herein.
In one aspect, the present invention relates to the use of pharmaceutical compositions comprising as an active ingredient a peptide indicated by the following formula (I) or a salt thereof:
wherein:
A1 in the above-mentioned formula (I) represents an arginine, lysine, ornithine, citrulline, alanine or glutamic acid residue (either L or D form) which may be derivatized at the N-terminus, or A1 is a hydrogen atom, or it is preferable that A1 is an arginine, citrulline, alanine or D-glutamic acid residue, or A1 is a hydrogen atom (i.e. the amino acid at this position may be absent).
Examples of “N-terminal derivatized peptides” or “N-α-substituted derivatives” include, but are not limited to, those protected by formyl group; acyl group, e.g., acetyl group, propionyl group, butyryl group, pentanoyl group, C2-6alkanoyl group e.g. hexanoyl group, benzoyl group, arylcarbonyl group e.g. substituted benzoyl group (e.g.:
2-fluorobenzoyl, 3-fluorobenzoyl group, 4-fluorobenzoyl group, 2-bromobenzoyl group, 3-bromobenzoyl group, 4-bromobenzoyl group, 2-nitrobenzoyl group, 3-nitrobenzoyl group, 4-nirtobenzoyl group), succinyl group, glutaryl group; nicotinyl group; isonicotinyl group; alkylsulfonyl group (e.g.: methanesulfonyl group, ethanesulfonyl group, propanesulfonyl group, camphorsulfonyl group); arylsulfonyl group (e.g.: p-toluenesulfonyl group, 4-fluorobenzenesufonyl group, mesitylenesulfonyl group, 4-aminobenzenesulfonyl group, dansyl group, 4-bromobenzenesulfonyl group) etc. Or, the N-terminal amino acid group may be absent.
A2 in the above-mentioned formula (I) represents an arginine or glutamic acid residue (either L or D form) if A1 is an arginine, lysine, ornithine, citrulline, alanine or glutamic acid residue (either L or D form) which may be derivatized at the N-terminus, or A2 represents an arginine or glutamic acid residue (either L or D form) which may be derivatized at the N-terminus if A1 is absent, or it is preferable that A2 is an arginine or glutamic acid residue if A1 is an arginine, citrulline, alanine or glutamic acid residue which may be derivatized at the N-terminus, or A2 is an arginine or glutamic acid residue which may be derivatized at N-terminus if A1 is absent. Examples of “peptides derivatized at the N-terminus” include, but are not limited to, the same ones as those mentioned in A1.
A3 in the above-mentioned formula (I) represents an aromatic amino acid residue (e.g., phenylalanine, tryptophan, 3-(2-naphthyl)alanine, tyrosine, 4-fluorophenylalanine, 3 -(1 -naphthyl)alanine (either L or D form), or preferably, A3 represents phenylalanine, tryptophan or 3-(2-naphthyl)alanine.
A4 in the above-mentioned formula (I) represents an arginine, lysine, ornithine, citrulline, alanine or glutamic acid residue (either L or D form), or it is preferable that A4 is an arginine, citrulline, alanine or L- or D-glutamic acid residue.
A5 in the above-mentioned formula (I) represents an arginine, lysine, ornithine, citrulline, alanine or glutamic acid residue (either L or D form), or it is preferable that A5 is an arginine, citrulline, alanine, lysine or glutamic acid residue.
A6 in the above-mentioned formula (I) represents a proline, glycine, ornithine, lysine, alanine, citrulline, arginine or glutamic acid residue (either L or D form), or it is preferable that A6 is a D-lysine, D-alanine, D-citrulline or D-glutamic acid residue.
A7 in the above-mentioned formula (I) represents a proline, glycine, ornithine, lysine, alanine, citrulline or arginine residue (either L or D form), or it is preferable that A7 is a proline or alanine residue.
A8 in the above-mentioned formula (I) represents a tyrosine, phenylalanine, alanine, naphthylalanine, citrulline or glutamic acid residue (either L or D form), or it is preferable that A8 is a tyrosine, alanine or D-glutamic acid residue.
A9 in the above-mentioned formula (I) represents an arginine, lysine, ornithine, citrulline, alanine or glutamic acid residue (either L or D form), or it is preferable that A9 is an arginine, citrulline or glutamic acid residue.
A10 in the above-mentioned formula (I) represents a citrulline, glutamic acid, arginine or lysine residue (either L or D form), or it is preferable that A10 is a citrulline or D-glutamic acid residue.
A11 in the above-mentioned formula (I) represents an arginine, glutamic acid, lysine or citrulline residue (either L or D form) which may be derivatized at C-terminus, or it is preferable that A11 is an arginine or glutamic acid residue which may be derivatized at the C-terminus
“C-terminal derivatization” or “C-terminal carboxyl derivatization” includes, without limitation, amidation (—CONH2, —CONHR, —CONRR′) and esterification (—COOR). Herein, R and R′ in amides and esters include, for example, C1-6 alkyl group e.g. methyl, ethyl, n-propyl, isopropyl, or n-butyl, C3-8 cycloalkyl group e.g. cyclopentyl, cyclohexyl, C6-12 aryl group e.g. phenyl and a-naphthyl, phenyl-C1-2 alkyl group e.g. benzyl, phenethyl or C7-14 aralkyl group e.g. C1-2 alkyl group e.g. α-naphthyl methyl group, and additionally, pivaloyloxymethyl group which is generally used as an oral bioavailable ester.
If a peptide of the present invention has carboxy groups (or carboxylates) at side-chain terminals other than C-terminus, the peptide having amidated or esterificated carboxy groups at side-chain terminals is included in the peptides of the present invention. As the amides and esters in this case, for example, the amides and esters exemplified in A11 are similarly used. Also, the peptides of the present invention include peptides in which substituents (e.g. —OH, —SH, amino group, imidazole group, indole group, guanidino group, etc.) on the intramolecular amino acid side chains are protected by suitable protective group (e.g. C1-6 acyl group, C2-6 alkanoyl such as formyl group, acetyl group, etc.), or complex peptides such as glycopeptides combined with sugar chain in the above-mentioned peptides.
Salts of the peptides of the present invention include physiologically acceptable salts of acids or bases and particularly, physiologically acceptable acid addition salts are preferable. Such salts are exemplified by salts of inorganic acids (e.g. hydrochloric acid, phosphoric acid, hydrobromic acid, sulfuric acid), or salts of organic acids (e.g. acetic acid, formic acid, propionic acid, fumaric acid, maleic acid, succinic acid, tartaric acid, citric acid, malic acid, oxalic acid, benzoic acid, methanesulfonic acid, benzenesulfonic acid).
In one embodiment, the composition comprises a peptide as set forth in formula (I) as defined hereinabove, wherein A1 is a glutamic acid residue or is absent (not present).
In another embodiment, the composition comprises a peptide as set forth in formula (I) as defined hereinabove, wherein A4 is a glutamic acid residue.
In another embodiment, the composition comprises a peptide as set forth in formula (I) as defined hereinabove, wherein A6 is a glutamic acid residue.
In another embodiment, the composition comprises a peptide as set forth in formula (I) as defined hereinabove, wherein A8 is a glutamic acid residue.
In another embodiment, the composition comprises a peptide as set forth in formula (I) as defined hereinabove, wherein A9 is a glutamic acid residue.
In another embodiment, the composition comprises a peptide as set forth in formula (I) as defined hereinabove, wherein A5 is an arginine or glutamic acid residue.
In another embodiment, the composition comprises a peptide as set forth in formula (I) as defined hereinabove, wherein A10 is a glutamic acid, arginine or lysine residue.
In another embodiment, the composition comprises a peptide as set forth in formula (I) as defined hereinabove, wherein A11 is a glutamic acid, lysine or citrulline residue.
In another embodiment, the peptide has an amino acid sequence as set forth in any one of SEQ ID NOS: 1-72 presented in Table 1 herein:
In each one of SEQ ID NOS: 1-72, two cysteine residues are preferably coupled in a disulfide bond. Currently preferred peptides according to the present invention are peptides having an amino acid sequence as set forth in any one of SEQ ID NOS: 1-72, wherein each possibility represents a separate embodiment of the present invention.
In another particular embodiment, the peptide used in the compositions and methods of the invention consists essentially of an amino acid sequence as set forth in SEQ ID NO: 1. In another preferable embodiment, the peptide used in the compositions and methods of the invention is of an amino acid sequence as set forth in SEQ ID NO:1. In another embodiment, the peptide (analog) is at least 60%, preferably at least 70% and more preferably at least 80% homologous to SEQ ID NO: 1. In another embodiment, the peptide is at least about 90% homologous to SEQ ID NO:1. In another embodiment, the peptide is at least about 95% homologous to SEQ ID NO: 1. Each possibility represents a separate embodiment of the present invention.
It is generally accepted, that the degree of homology between two sequences depends on both the degree of identity in their amino acid sequences and their identity with respect to their length. The peptide homologs of the invention are thus typically about 8-22 amino acids in length, more typically 14-20 amino acid in length or in other embodiments 13-15 amino acids in length, and in particular embodiments about 14 amino acids in length. In various other particular embodiments, the peptide is selected from SEQ ID NOS: 1-72, wherein each possibility represents a separate embodiment of the present invention.
In another particular embodiment, said peptide has an amino acid sequence as set forth in any one of SEQ ID NOS: 1-4, 10, 46, 47, 51-56, 65, 66, 68, 70 and 71. In another particular embodiment, said peptide has an amino acid sequence as set forth in any one of SEQ ID NOS: 4, 10, 46, 47, 68 and 70. In another particular embodiment, said peptide has an amino acid sequence as set forth in any one of SEQ ID NOS: 1, 2, 51, 65 and 66. In another particular embodiment, said peptide has an amino acid sequence as set forth in any one of SEQ ID NOS: 53-56. Each possibility represents a separate embodiment of the invention.
In a preferable particular embodiment, said peptide has an amino acid sequence as set forth in SEQ ID NO: 1. In another particular embodiment, said peptide has an amino acid sequence as set forth in SEQ ID NO:2. In another particular embodiment, said peptide has an amino acid sequence as set forth in SEQ ID NO: 51. In another particular embodiment, said peptide has an amino acid sequence as set forth in SEQ ID NO: 66. Each possibility represents a separate embodiment of the invention.
In another aspect, the invention relates to the use of a pharmaceutical composition comprising a peptide indicated by the following formula (II) or a salt thereof:
wherein:
wherein A8 and A12 each independently represents an alanine, valine, leucine, isoleucine, serine, cysteine or methionine residue;
In particular embodiments the peptides of the formula (II) are peptides wherein A1, A6 and A7 are arginine residues, A2 is a naphthylalanine residue, A3 is a citrulline residue, A4 is a lysine residue, X is a D-lysyl-proline residue, and A5 is a tyrosine residue, a polypeptide of the formula (II) wherein A1, A3, A6 and A7 are arginine residues, A2 is a naphthylalanine residue, A4 is a lysine residue, X is a D-citrullyl-proline residue, and A5 is a tyrosine residue, a polypeptide of the formula (II) wherein A1, A6 and A7 are arginine residues, A2 is a naphthylalanine residue, A3 is a citrulline residue, A4 is a lysine residue, X is a D-citrullyl-proline residue, A5 is a tyrosine residue, and a polypeptide of the formula (II) wherein A1 is a citrulline residue, A2 is a naphthylalanine residue, A3, A6 and A7 are arginine residues, A4 is a lysine residue, X is a D-citrullyl-proline residue, A5 is a tyrosine residue.
The peptides of formula (II) may be exemplified in another embodiment by a peptide of the formula (II) wherein A1, A6 and A7 are arginine residues, A2 is a naphthylalanine residue, A3 is a alanine residue, A4 is a lysine residue, X is a D-lysyl-proline residue, and A5 is a tyrosine residue, a polypeptide of the formula (II) wherein A1 is a citrulline residue, A2 is a naphthylalanine residue, A3, A6 and A7 are arginine residues, A4 is a lysine residue, X is a D-lysyl-proline residue, and A5 is a tyrosine residue, a polypeptide of the formula (II) wherein A1, A3 and A7 are arginine residues, A2 is a naphthylalanine residue, A4 is a lysine residue, X is a D-lysyl-proline residue, A5 is a tyrosine residue, and A6 is a citrulline residue, a polypeptide of the formula (II) wherein A1 and A3 are citrulline residues, A2 is a naphthylalanine residue, A4 is a lysine residue, X is a D-lysyl-proline residue, A5 is a tyrosine residue, A6 and A7 are arginine residues, and a polypeptide of the formula (II) wherein A1, A3 and A7 are arginine residues, A2 is a naphthylalanine residue, A4 is a lysine residue, X is a D-citrullyl-proline residue, A5 is a tyrosine residue, and A6 is a citrulline residue.
The amino acid of A7 as presented in formula II herein is preferably one in which the carboxyl group is amidated for improving stability of the polypeptide in vivo such as in serum, etc.
A peptide of the present invention includes a peptide or its amide, ester or salt containing the amino acid sequence which is substantially the same amino acid to sequence as the sequence of any of the above-mentioned peptides. Here, “substantially the same amino acid sequence” means an amino acid sequence that is qualitatively identical in the activity of the peptide or the biological activity of the peptide (e.g. inhibit large cell lung cancer tumor cells growth and/or induce their death) or the like. Accordingly, quantitative variances are acceptable to some extent (e.g. about 0.01 to 100 times, preferably 0.5 to 20 times, or more preferably 0.5 to 2 times). Therefore, one or more of the amino acids in the amino acid sequences indicated in any of the above-mentioned formula (I), (II) and SEQ ID NOS: 1-72 can have variances, so far as they have any of the above-mentioned properties. That is to say, in the present invention, any peptide (variant peptide) resulting from the variance in the amino acid sequence such as substitution, deletion or insertion (addition) etc. which brings about no significant change (i.e. a qualitatively different change, or a qualitatively identical but quantitatively significantly different change) in the physiological property or chemical property of the original (non-variant) peptide is deemed as substantially the same as the original (non-variant) peptide having no such variance, and, the amino acid sequence of such variant peptide is deemed as substantially the same as the amino acid sequence of the original (non-variant) peptide.
It is a well-known fact that generally, the changes such as substitution, deletion or insertion (addition) of an amino acid in a peptide sequence often do not make a significant change to physiological properties or chemical properties of such peptide. For example, it is generally considered that substitution of a certain amino acid by another amino acid of similar chemical properties results in a peptide having minimized deviation from the properties of the original peptide.
Amino acids are classified, using the similarity of their properties as to one of the criteria, into the following classes, for example: (i) nonpolar (hydrophobic) amino acids (examples: alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, methionine, etc.); (ii) polar (neutral) amino acids (examples: glycine, serine, threonine, cysteine, tyrosine, asparagine, glutamine, etc.); (iii) basic amino acids carrying positive electric charge (examples: arginine, lysine, histidine, etc.); (iv) acidic amino acids carrying negative electric charge (examples: asparatic acid, glutamic acid, etc.), and accordingly, amino acid substitution within each class can be conservative with regard to the property of a peptide (namely, substitution generating “substantially same” amino acid sequences). In other words, “substantially the same amino acid sequences” may include:
(i) amino acid sequences wherein 1 or more, or, in other embodiments, 1 to 3 amino acids were substituted by other amino acids in the amino acid sequences indicated in the above-mentioned formula (I), (II) and SEQ ID NOS:1-72;
(ii) amino acid sequences wherein 1 or more, or, in other embodiments, 1 to 3 amino acids were deleted in the amino acid sequences indicated in the above-mentioned formula (I), (II) and SEQ ID NOS:1-72;
(iii) amino acid sequences wherein 1 or more or, in other embodiments, 1 to 3 amino acids were added (inserted) in the amino acid sequences indicated in the above-mentioned formula (I), (II) and SEQ ID NOS:1-72; or
(iv) peptides including modifications to amino acids (particularly, the side chains thereof) among the peptides having the amino acid sequences indicated in above (i), (ii) or (iii), or esters, amides or salts thereof.
A peptide of the present invention, if and when the substitution, deletion, insertion (addition), modification, etc. of above (i) to (iv) is intentionally or incidentally provided in the amino acid sequence thereof, can be varied to a stable peptide against heat or protease or a high-activity peptide having more enhanced activity. The peptides of the present invention include also these variant peptides or amides thereof, esters thereof or salts thereof.
Furthermore, among the peptides of the present invention are the peptide consisting of the amino acid sequence indicated in any of the above-mentioned formula (I), (II) and SEQ ID NOS:1-72, and the peptide containing the amino acid sequence sharing the homology of about 50 to 99.9% (preferably, 70 to 99.9%, more preferably 90 to 99.9%) with the foregoing amino acid sequence and having the activities of substantially the same nature as the peptide consisting of the amino acid sequence indicated in any of the above-mentioned formula (I), (II) and SEQ ID NOS:1-72, or amides thereof, esters thereof or salts thereof.
Peptide analogs of the invention include in other embodiments peptides which are identical to SEQ ID NO: 1 or other peptides disclosed herein with respect to their amino acid sequence but have different derivatizing groups (e.g. N′ derivatization or C′ derivatization), as long as they are qualitatively identical in their anti-tumor activity as the peptides disclosed herein.
The amides, esters or salts of the peptide having the amino acid sequence indicated in any of the above-mentioned SEQ ID NOS: 1-72 include the same ones as are exemplified for the peptide indicated in the above-mentioned formula (I). Preferably, the peptide having the amino acid sequence indicated in any of the above-mentioned SEQ ID NOS: 1-72 is amidated at the carboxyl group of the C-terminal amino acid residue.
The peptides of the present invention including the peptide containing the amino acid sequence indicated in any of the above-mentioned SEQ ID NOS: 1-72 can be produced by conventionally known methods of synthesizing peptides. For the syntheses of peptides, either solid phase peptide synthesis or liquid phase synthesis may be utilized. Namely, an expected peptide can be produced by condensing a partial peptide able to constitute a peptide or an amino acid with remaining portions, and if the product has a protecting group, by eliminating the protecting group. As the known condensation methods and elimination of protecting groups, the following examples (1) to (5) are included:
As practical methods for syntheses of peptides, the following examples can be given:
Generally, commercially available resins for synthesis of polypeptides can be used. Such resins include, for example, chloromethyl resin, hydroxymethyl resin, benzhydroxylamine resin, aminomethyl resin, 4-hydroxybenzylalcohol resin, 4-methylbenzhydroxylamine resin, PAM resin, 4-hydroxymethylmethylphenylacetoamidomethyl resin, polyacrylamide resin, 4-(2′,4′-dimetoxyphenyl-hydroxymethyl)phenoxy resin, 4-2′,4′ -dimetoxyphenyl- Fmoc aminoethylphenoxy resin, etc. Using such resin, an amino acid with suitably protected α-amino group and side chain functional group is condensed on the resin to the sequence of the expected polypeptide in accordance with conventionally known condensation methods. In the last stage of the reaction, the polypeptide is cleared from the resin and simultaneously various protective groups are removed, and then, by carrying out intramolecular disulfide bond-forming reaction in highly diluted solution, the expected polypeptide or amide thereof is obtained. For the above-mentioned condensation of the protected amino acid, various activated reagents usable for the syntheses of polypeptides can be used, but it is particularly better to use carboxyimides. Among such carboxyimides are DCC, N,N′-diisopropylcarbodiimide, N-ethyl-N′-(3-dimethylaminopropyl)cabodiimde, etc. For the activation by these, together with racemization inhibitory additives (for example, HOBt, HOOBt), a protected amino acid is added directly to the resin, or after activating the protected amino acid as symmetric acid anhydride or HOBt ester or HOOBt ester, it can be added to ester resin.
Solvents used for the activation of protected amino acids and the condensation with resins can be chosen from among the solvents known to be usable for polypeptide condensation reactions. For example, acid amides such as N,N-dimethylformamide, N,N-dimethylacetoamide and N-methylpyrrolidone, halogenated hydrocarbons such as methylene chloride and chloroform, alcohols such as trifluoroethanol, sulfoxides such as methyl sulfoxide, ethers such as pyridine, dioxane and tetrahydrofuran, nitriles such as acetonitrile and propionitrile, esters such as methyl acetate and ethyl acetate, or appropriated mixtures of the foregoing are used. A solvent used for activation of a protected amino acid or its condensation with resin can be selected from among the solvents known to be usable for condensing reactions of polypeptides. The reaction temperature is appropriately set within the scope known to be applicable to polypeptide bond forming reactions, usually, at −20° C. to 50° C. Activated amino acid derivatives are usually used at 1.5 to 4 times excess. According to the result of tests adopting ninhydrin reaction, if the condensation is insufficient, the repetition of condensation reactions without eliminating protective groups can lead to sufficient condensation. If sufficient condensation is attained by the repetition of reactions, unreacted amino acids can be acetylated by the use of acetic anhydride or acetylimidazole.
The protective group of the amino group used as ingredients include, for example, Z, Boc, tertialypentyloxycarbony, isobornyloxycarbonyl, 4-methoxybenzyloxycabonyl, Cl—Z, Br—Z, adamantyloxycabonyl, trifluoroacetyl, phtaloyl, formyl, 2-nitrophenylsulphenyl, diphenylphosphinothioyl, Fmoc, etc. Carboxyl group can be protected, for example, by alkyl esterification (e.g. straight-chain, branching or circular alkyl esterification of methyl, ethyl, propyl, butyl, tertialbutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, 2-adamantyl, etc.), aralkyl esterification (e.g. benzylester, 4-nitrobenzylester, 4-methoxybenzylester, 4-chlorbenzylester, benzhydryl esterification), phenacylesterification, benzylcarbonylhydrazidation, tertialybutoxycarbonylhydrazidation, tritylhydrazidation, etc. The hydroxyl group of serine can be protected, for example, by esterification or etherification. The groups suitable for this esterification include, for example, groups derivatized from carboxylic acid such as lower alkanoyl group such as acetyl group, aroyl group such as benzoyl group, benzyloxycarbonyl group, ethoxycarbonyl group. The groups suitable for etherification include, for example, benzyl group, tetrahydropiranyl group, tertiarybutyl group, etc. As the protective groups of phenolic OH group of tyrosine, for example, Bzl, C12-Bzl, 2-nitrobenzyl, Br-Z, tertiarlybutyl, etc. are used. As the protective groups of imidazole of histidine, for example, Tos, 4-methoxy-2,3,6-trimethylbenzenesulfonyl, DNP, benzyloxymethyl, Bum, Boc, Trt, Fmoc etc. are used.
Ingredients with activated carboxyl groups include, for example, corresponding acid anhydride, azide, active ester [ester of alcohol (e.g. pentachlorophenol, 2,4,5-trichlorophenol, 2,4-dinitrophenol, cyanomethylalcohol, p-nitrophenol, HONB, N-hydroxysuccimide, N-hydroxyphtalimide, HOBO] are used. Ingredients with activated amino group include, for example, corresponding phosphoric amide. As the methods to remove (eliminate) protective groups, for example, catalytic reduction in hydrogen airstream in the presence of a catalyst such as Pd-black or Pd-carbon, acid treatment by anhydrous hydrogen fluoride, methanesulfonic acid, trifluoromethanesulfonic acid, trifluoroacetic acid or a mixture thereof, etc, base treatment by diisopropylethylamine, triethylamine, piperidine, piperadine, etc., and reduction by natrium in liquid ammonia are used. Elimination reaction by the above-mentioned acid treatment is done generally at the temperature of about −20° C. to 40° C., but in the acid treatment, it is effective to add a cation trapping agent such as anisole, phenol, thioanisole, m-cresol, p-cresol, dimethylsulfide, 1,4-butanedithiol, 1,2-ethanedithiol. 2,4-dinitrophenyl group used as the protective group of imidazole of histidine is removed by thiophenol treatment. Formyl group used as the protective group of indole of tryptophan is removed by elimination of protection by the above-mentioned acid treatment in the presence of 1,2-ethanedithiol, 1,4-butanedithiol, etc. and also is removed by alkaline treatment by dilute sodium hydroxide solution, dilute ammonia, etc.
Protection and protective group of functional groups not to be involved in the reaction of ingredients, and elimination of such protective group, and activation of functional groups to be involved in the reaction, etc. can be appropriately selected from among conventionally known groups or conventionally known measures. As alternative methods to obtain amides of polypeptides, there is, for example, a method to manufacture, after amidating and protecting a-carboxyl group of carboxy-terminal amino acid and then extending the peptide chain to the desired chain length on the side of amino group, a polypeptide eliminating the protective group of a-amino group of the N-terminus of such peptide chain and a polypeptide eliminating the protective group of carboxyl group of the C-terminus, and then these two peptides are condensed in the above-mentioned mixed solvent. The details of the condensation reaction are the same as described above. After purifying the protected polypeptide obtained by the condensation, the desired raw polypeptide can be obtained by eliminating all the protective groups by the above-mentioned method. Having purified this raw polypeptide using various known purification methods, if the main fraction is freeze-dried, an amide type of the desired polypeptide can be obtained. To get an ester type of the polypeptide, for example, make an amino acid ester by condensing a-carboxyl group of carboxy-terminal amino acid with the desired alcohols, and then, the ester type of the desired polypeptide can be obtained in the same way as the amide type of the polypeptide.
After the reaction, the peptides of the present invention can be purified and isolated by combining usual purification methods such as solvent extraction, distillation, column chromatography, liquid chromatography, re-crystallization, etc. If a peptide obtained by the above-mentioned methods is a salt-free type, it can be converted to a suitable salt by known methods, or if such peptide is a salt, it can be converted to a salt-free type by known methods.
As mentioned, the peptides of the invention are used for the treating of large cell to lung cancer.
As used herein the phrase “large cell lung cancer” or “large cell carcinomas” are tumors of the lung having large round cells when examined under the microscope, although the tumors themselves tend to be large as well when diagnosed. Large cell carcinomas often occur in the outer regions of the lungs, and tend to grow rapidly and spread more quickly than some other forms of non-small cell lung cancer.
Large cell lung carcinomas constitute only about 10-15% of the non-small cell lung cancers. Large cell carcinomas include all lung cancers that cannot be classified as non small cell lung cancer classified as adenocarcinoma and sequamouns cell carcinoma. The level of CXCR4 expression on the tumor cells can be used to corroborate the type of cancer (see Example 1 of the Examples section, which follows).
As used herein, the term “treating” refers to inhibiting, preventing or arresting the development of a pathology (disease, disorder or condition i.e., large cell lung cancer) and/or causing the reduction, remission, or regression of a pathology. Those of skill in the art will understand that various methodologies and assays can be used to assess the development of a pathology, and similarly, various methodologies and assays may be used to assess the reduction, remission or regression of a pathology.
As used herein, the term “preventing” refers to keeping a disease, disorder or condition from occurring in a subject who may be at risk for the disease, but has not yet been diagnosed as having the disease.
Methods of diagnosing and monitoring large cell lung cancer are well known in the art. Standard methods which are used include, but are not limited to, X-ray or imaging including, but not limited to, PET scan, CT scan. Biopsies of lung tissue can be used to confirm the diagnosis.
As used herein, the term “subject” includes mammals, preferably human beings at any age which suffer from the pathology.
The peptides of some embodiments of the invention can be administered to the subject per se, or in a pharmaceutical composition where it is mixed with suitable carriers or excipients.
As used herein a “pharmaceutical composition” refers to a preparation of one or more of the active ingredients described herein with other chemical components such as physiologically suitable carriers and excipients. The purpose of a pharmaceutical composition is to facilitate administration of a compound to an organism.
Herein the term “active ingredient” refers to the peptides accountable for the biological effect. Optionally, a plurality of active ingredient may be included in the formulation such as chemotherapy, radiation agents and the like, as further described hereinbelow.
Hereinafter, the phrases “physiologically acceptable carrier” and “pharmaceutically acceptable carrier”, which may be used interchangeably, refer to a carrier or a diluent that does not cause significant irritation to an organism and does not abrogate the biological activity and properties of the administered compound.
Herein, the term “excipient” refers to an inert substance added to a pharmaceutical composition to further facilitate administration of an active ingredient. Examples, without limitation, of excipients include calcium carbonate, calcium phosphate, various sugars and types of starch, cellulose derivatives, gelatin, vegetable oils, and polyethylene glycols.
Techniques for formulation and administration of drugs may be found in the latest edition of “Remington's Pharmaceutical Sciences”, Mack Publishing Co., Easton, Pa., which is herein fully incorporated by reference (Remington: The Science and Practice of Pharmacy, Gennaro, A., Lippincott, Williams & Wilkins, Philadelphia, Pa., 20th ed, 2000).
Pharmaceutical compositions of the present invention may be manufactured by processes well known in the art, e.g., by means of conventional mixing, dissolving, granulating, dragee-making, levigating, emulsifying, encapsulating, entrapping, or lyophilizing processes.
Pharmaceutical compositions for use in accordance with the present invention thus may be formulated in conventional manner using one or more physiologically acceptable carriers comprising excipients and auxiliaries, which facilitate processing of the active ingredients into preparations that can be used pharmaceutically. Proper formulation is dependent upon the route of administration chosen.
The pharmaceutical compositions of the invention are suitable for administration systemically or in a local manner, for example, via injection of the pharmaceutical composition directly into a tissue region of a patient. In a preferred embodiment, the peptide or the pharmaceutical compositions comprising same is administered locally directly into the tumor i.e., intratumorally.
For injection, the active ingredients of the pharmaceutical composition may be formulated in aqueous solutions (e.g., WFI), preferably in physiologically compatible buffers such as Hank's solution, Ringer's solution, or physiological salt buffer.
Pharmaceutical compositions for potential administration include aqueous solutions of the active preparation in water-soluble form. Additionally, suspensions of the active ingredients may be prepared as appropriate oily or water-based injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters such as ethyl oleate, triglycerides, or liposomes. Aqueous injection suspensions may contain substances that increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Optionally, the suspension may also contain suitable stabilizers or agents that increase the solubility of the active ingredients, to allow for the preparation of highly concentrated solutions.
Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., a sterile, pyrogen-free, water-based solution, before use.
For oral administration, the pharmaceutical composition can be formulated readily by combining the active compounds with pharmaceutically acceptable carriers well known in the art. Such carriers enable the pharmaceutical composition to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions, and the like, for oral ingestion by a patient. Pharmacological preparations for oral use can be made using a solid excipient, optionally grinding the resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries as desired, to obtain tablets or dragee cores. Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, and sodium carbomethylcellulose;
and/or physiologically acceptable polymers such as polyvinylpyrrolidone (PVP). If desired, disintegrating agents, such as cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof, such as sodium alginate, may be added.
Dragee cores are provided with suitable coatings. For this purpose, concentrated to sugar solutions may be used which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, carbopol gel, polyethylene glycol, titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.
Pharmaceutical compositions that can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol. The push-fit capsules may contain the active ingredients in admixture with filler such as lactose, binders such as starches, lubricants such as talc or magnesium stearate, and, optionally, stabilizers. In soft capsules, the active ingredients may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols. In addition, stabilizers may be added. All formulations for oral administration should be in dosages suitable for the chosen route of administration.
For buccal administration, the compositions may take the form of tablets or lozenges formulated in conventional manner.
Alternative embodiments include depots providing sustained release or prolonged duration of activity of the active ingredient in the subject, as are well known in the art.
Pharmaceutical compositions suitable for use in the context of the present invention include compositions wherein the active ingredients are contained in an amount effective to achieve the intended purpose. Determination of a therapeutically effective amount is well within the capability of those skilled in the art, especially in light of the detailed disclosure provided herein.
For any preparation used in the methods of the invention, the therapeutically effective amount or dose can be estimated initially from in vitro and cell culture assays. For example, a dose can be formulated in animal models to achieve a desired concentration or titer. Such information can be used to more accurately determine useful doses in humans.
Toxicity and therapeutic efficacy of the active ingredients described herein can be determined by standard pharmaceutical procedures in vitro, in cell cultures or experimental animals (see the Examples section which follows, and Sekido et al. 2002 Cancer Genet Cytogenet 137(1):33-42). The data obtained from these in vitro and cell culture assays and animal studies can be used in formulating a range of dosage for use in human. The dosage may vary depending upon the dosage form employed and the route of administration utilized. The exact formulation, route of administration and dosage can be chosen by the individual physician in view of the patient's condition. (See e.g., Fingl, et al., 1975, in “The Pharmacological Basis of Therapeutics”, Ch. 1 p. 1).
Exemplary doses of the peptide of the invention for human use may be in some embodiments 0.03-10 mg/kg, 0.1-10 mg/kg, 0.1-2 mg/kg, 0.1-1 mg/kg, 0.3-10 mg/kg, 0.3-2 mg/kg, 0.3-1 mg/kg or 0.3-0.9 mg/kg.
The peptides of the current invention derivatives or analogs thereof can be delivered in a controlled release system. Thus, an implant (e.g., an infusion pump) can be used to administer the peptide such as the one that is used, for example, for delivering insulin or chemotherapy to specific organs or tumors. In one embodiment, the peptide of the invention is administered in combination with a biodegradable, biocompatible polymeric implant, which releases the peptide over a controlled period of time at a selected site. Examples of preferred polymeric materials include, but are not limited to, polyanhydrides, polyorthoesters, polyglycolic acid, polylactic acid, polyethylene vinyl acetate, copolymers and blends thereof (See, Medical applications of controlled release, Langer and Wise (eds.), 1974, CRC Pres., Boca Raton, Fla., the contents of which are hereby incorporated by reference in their entirety). In yet another embodiment, a controlled release system can be placed in proximity to a therapeutic target, thus requiring only a fraction of the systemic dose.
As mentioned hereinabove, in other embodiments, the peptides may be used in combination with anti-cancer treatments, e.g. with one or more chemotherapeutic drugs.
According to an exemplary embodiment, the chemotherapeutic agent is an alkylating-like agent such as Cisplatin, Carboplatin and Nedaplatin.
According to another exemplary embodiment, the chemotherapeutic agent is a mitotic inhibitor such a paclitaxel and docetaxel.
In another embodiment, the compositions and methods of the invention enhance the effectiveness of chemotherapy in a subject afflicted with cancer. Alternatively or additionally, the peptide of the invention is administered along with (or complements) radiation treatment.
Thus, according to a further embodiment of the invention, there is provided an to article of manufacture or a kit comprising a packaging that includes in a first container the peptide of the invention and in another container at least one chemotherapy agent or anti-cancer agent. The article-of-manufacture comprises a label with identification and possibly instructions for the treatment of large cell lung cancer.
In yet another embodiment, the composition or treatment regimen consists of a peptide of the invention as a sole active ingredient.
Compositions of some embodiments of the invention may, if desired, be presented in a pack or dispenser device, such as an FDA approved kit, which may contain one or more unit dosage forms containing the active ingredient. The pack may, for example, comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration. The pack or dispenser may also be accommodated by a notice associated with the container in a form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceuticals, which notice is reflective of approval by the agency of the form of the compositions or human or veterinary administration. Such notice, for example, may be of labeling approved by the U.S. Food and Drug Administration for prescription drugs or of an approved product insert. Compositions comprising a preparation of the invention formulated in a compatible pharmaceutical carrier may also be prepared, placed in an appropriate container, and labeled for treatment of an indicated condition, as is further detailed above.
As used herein the term “about” refers to ±10%.
The terms “comprises”, “comprising”, “includes”, “including”, “having” and their conjugates mean “including but not limited to”.
The term “consisting of” means “including and limited to”.
The term “consisting essentially of” means that the composition, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure.
As used herein, the singular form “a”, “an” and “the” include plural references unless the context clearly dictates otherwise. For example, the term “a compound” or “at least one compound” may include a plurality of compounds, including mixtures thereof.
Throughout this application, various embodiments of this invention may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.
Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween.
As used herein the term “method” refers to manners, means, techniques and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the chemical, pharmacological, biological, biochemical and medical arts.
It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential features of those embodiments, unless the embodiment is inoperative without those elements.
Various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below find experimental support in the following examples.
Reference is now made to the following examples, which together with the above descriptions illustrate the invention in a non limiting fashion.
Generally, the nomenclature used herein and the laboratory procedures utilized in the present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, “Molecular Cloning: A laboratory Manual” Sambrook et al., (1989); “Current Protocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., “Current Protocols in Molecular Biology”, John Wiley and Sons, Baltimore, Maryland (1989); Perbal, “A Practical Guide to Molecular Cloning”, John Wiley & Sons, New York (1988); Watson et al., “Recombinant DNA”, Scientific American Books, New York; Birren et al. (eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis, J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-III Coligan J. E., ed. (1994); Stites et al. (eds), “Basic and Clinical Immunology” (8th Edition), Appleton & Lange, Norwalk, CT (1994); Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W. H. Freeman and Co., New York (1980); available immunoassays are extensively described in the patent and scientific literature, see, for example, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521; “Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., eds. (1985); “Transcription and Translation” Hames, B. D., and Higgins S. J., Eds. (1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “Immobilized Cells and Enzymes” IRL Press, (1986); “A Practical Guide to Molecular Cloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317, Academic Press; “PCR Protocols: A Guide To Methods And Applications”, Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategies for Protein Purification and Characterization—A Laboratory Course Manual” CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference.
Materials and Experimental Procedures
Cell Lines
The H460 (large cell carcinoma), A549 (Adenocarcinoma), H358 (Bronchoalveolar carcinoma), H1299 (large cell carcinoma)—cell lines were all purchased from ATCC and were maintained in RPMI (Gibco Laboratories, Grand Island, N.Y.) containing 10% fetal calf serum (FCS), 1 mM L-glutamine, 100 U/ml penicillin, and 0.01 mg/ml streptomycin (Biological Industries, Kibbutz Beth Haemek, Israel). L-4 (a primary NSCLC cell line) was generated from large cell carcinoma as previously described (Wald et al, J Thorac Cardiovasc Surg. 2011 June; 141(6): 1503-12). All cell lines were tested for mycoplasma contamination and were found to be negative.
Flow Cytometry
Logarithmically growing cells were harvested, washed and 1*105 cells were stained with anti-human CXCR4 polyclonal anti-N-terminus antibody (Millipore) for 30 minutes at 4° C. PE-labelled donkey anti rabbit IgG (eBioscience) was used as secondary antibody, incubation time: 30 minutes at 4° C. Cells were then read and analyzed by FACS (Becton Dickinson Immunocytometry Systems), using CellQuest software. Control staining was obtained by adding IgG control or only the secondary antibody to the cells.
Colony Assays
Agar base layer was prepared as follows: 45 ml of RPMI+12% FCS was mixed with 15 ml of RPMI X2+12% FCS and with 15 ml of 2.5% agar in double distilled water. Tumor cells were suspended in RPMI+10% FCS. Cell suspension was mixed in a ratio of 1:3 with the agar base solution. This mixture was then plated on top of a preformed solid agar base. CXCL12, at the concentrations of 25 ng/ml, 50 ng/ml, 100 ng/ml and 250 ng/ml, with or without 100 μg\ml of BKT140, were added to the mixture. Fourteen days later, the number of colonies was counted in ten different fields.
Statistical Analysis
Data was expressed as mean ±standard error (SE) and as absolute values. Continuous data with normal distribution were analyzed using T-test. Categorical data were analyzed using Mann-Whitney U test. A p value of 0.05 was considered significant. * P<0.05, ** P<0.01. Statistical analyses were performed using (SPSS software, (IBM).
Results
Using flow cytometry, the expression of CXCR4 in five NSCLC cell lines (H358, A549, H460, H1299 and L4) was examined. As shown in
Materials and Experimental Procedures
Cell Lines
As described in Example 1, above.
Proliferation Assay
To measure cell proliferation, 4000 cells were seeded into 96-well cell culture plates with 200 μl RPMI 1640 medium supplemented with 10% FCS under the following conditions: 37° C., and 5% CO2. Twenty-four hours later, the medium was to changed to RPMI 1640 supplemented with 1% FCS. 20 μg\ml, 50 μg/ml or 100 μg\ml BKT140, with or without Cisplatin 0.1 μg/ml or paclitaxel 0.1 μg/ml to 1 μg/ml μg/ml were added to the medium. For radiation experiments, prior to plating of cells, the cells were irradiated at the following doses 500 to 2000 Rad. Cultured cells were fixed with 2.5% Gluteraldehyde at the indicated time points and stained with 1% Methylene Blue. Color extraction was done using 0.1 N HCl. Absorbance of the solution in each well at 570 nm was read with micro-plate reader (Anthos Htll). 6 wells were determined for each time point.
Survival Assay
1*105 cells\ml were cultured in flat bottom 24-well plates (Corning, N.Y.) with 1000 μl RPMI 1640 medium supplemented with 10% FCS under the following conditions: 37° C., and 5% CO2. 24 hours later, the medium was changed to RPMI 1640 supplemented with either 10% FCS or 1% FCS, with 20 μg\ml or 100 μg\ml BKT140. At indicated time points, cells were harvested, washed and stained with Propidium Iodide solution. The number of live cells was counted and analyzed by flow using FACS (Becton Dickinson Immunocytometry Systems) and CellQuest software.
Giemsa Stain
1*105 cells\ml were cultured in flat bottom 24-well plates (Corning, N.Y.) with 1000 μl RPMI 1640 medium supplemented with 10% FCS under the following conditions: 37° C., and 5% CO2. 24 hours later, the medium was changed to RPMI 1640 supplemented with 1% FCS with or without 100 μg\ml BKT140. At indicated time points, cells were fixed in 100% methanol for 30 minutes, washed in PBS and stained with 1:20 modified Giemsa stain (Sigma) for twenty minutes. Cells were then rinsed with tap water and images acquired.
Statistical Analysis
As described in Example 1, above.
Results
The potential of BKT140 to inhibit colony formation by NSCLC cell lines prompted to further study its potential anti-proliferative effects. To this end, NSCLC proliferation and survival in the presence or absence of increasing concentration of BKT140 was determined. As shown in
Materials and Experimental Procedures
Cell Lines
As described in Example 1, above.
In Vivo Experiments
1*106 H460 cells mixed in 100 μl RPMI 1640 and 100 μl matrigel (BD Bioscience) were injected subcutaneously (S.C.) into the right flank of Nude mice. Tumor growth was followed for 19 days. 1*106 A549 cells mixed in 100 μl RPMI 1640 and 100 μl matrigel (BD Bioscience) were injected S.C. into the right flank of Nude mice. Tumor growth was followed for 35 days. Systemic BKT140 administration was performed as follows: BKT140 (400 μg/mice dissolved in PBS) was administered once daily 6/7 days a week, by S.C. injection into the left flank at a point distant at least 1.5 cm from the right sided tumor. Tumors size was calculated by measurement of tumor length and width using the following formula: 4/3*π*(length/2)*(width/2)^2.
Immunohistochemistry
Tissue sections of tumor-bearing Nude mice were routinely fixed with formalin and embedded in paraffin. Antigen retrieval was performed, and sections were stained with either mAb 44708 (R&D Systems, Minneapolis, MN) or 12G5 (R&D Systems) for human CXCR4 (1:100) using a standard indirect avidin-biotin HRP method according to to the manufacturer's instructions. 3-Amino-9-ethylcarbazole was used for color development and sections were counterstained with hematoxylin. As negative controls, sections were stained either with no primary Ab (PBS) or with an isotype-matched control Ab.
Statistical Analysis
As described in Example 1, above.
Results
Once establishing the in vitro anti-proliferative effects of BKT140, these observations where further tested in vivo. The systemic effects and kinetics of S.C. injection of BKT140 were previously described (Abraham M, Beider K, Wald H, Weiss I D, Zipori D, Galun E, et al. The CXCR4 antagonist 4F-benzoyl-TN14003 stimulates the recovery of the bone marrow after transplantation. Leukemia 2009 August; 23(8):1378-88. For example, Abraham et al. reported an increase in mobilization of bone marrow derived cells peaking four to eight hours post S.C. injection of BKT140 and returning to base line at 24 hours post BKT140 injection (Abraham M, Beider K, Wald H, Weiss I D, Zipori D, Galun E, et al. The CXCR4 antagonist 4F-benzoyl-TN14003 stimulates the recovery of the bone marrow after transplantation. Leukemia 2009 August; 23(8):1378-88. The present results showed that BKT140, injected S.C. at a site located distant from the tumor, binds to tumor expressed CXCR4. Tissue sections from H460 derived tumors were tested for CXCR4 expression. Representative staining of tumor tissue with a control antibody and with an anti-CXCR4 antibody are shown in
Materials and Experimental Procedures
Cell Lines
As described in Example 1, above.
Proliferation Assay
As described in Example 2, above.
Statistical Analysis
As described in Example 1, above.
Results
As described in the background section above, currently practiced treatment protocols for advanced NSCLC involve the concomitant or staged administration of chemotherapeutic agents (e.g. cisplatin and paclitaxel) with radiation therapy. The gain of chemo and radio resistance by tumor cells is a key path underlining disease recurrence. Interference with the CXCR4/CXCL12 axis has recently been proposed as a potential path to follow in order to increase tumor sensitivity to chemotherapeutic agents and to radiotherapy. In this regard, the potential of BKT140 to enhance the cytostatic and cytotoxic effects of cisplatin, paclitaxel and radiotherapy on H460 and A549 cell lines was tested. First, proliferation of H460 and A549 cell lines following escalating doses of irradiation (
Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims.
All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference. In addition, citation or identification of any reference in this application shall not be construed as an admission that such reference is available as prior art to the present invention. To the extent that section headings are used, they should not be construed as necessarily limiting.
This application is a National Phase of PCT Patent Application No. PCT/IL2013/050352 having International filing date of Apr. 24, 2013, which claims the benefit of priority under 35 U.S.C. 517 119(e) of U.S. Provisional Patent Application Ser. No. 61/637,334 filed on Apr. 24, 2012. The contents of the above applications are all incorporated by reference as if fully set forth herein in their entirety.
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| Number | Date | Country | |
|---|---|---|---|
| 20150125549 A1 | May 2015 | US |
| Number | Date | Country | |
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| 61637334 | Apr 2012 | US |
