Claims
- 1. An isolated protein that has toxin activity against a corn rootworm pest wherein said protein is obtainable from Bacillus laterosporus isolate MB439 having accession number NRRL B-20086, said isolate comprising a DNA segment that encodes said toxin, wherein said DNA segment is selected from the group consisting of a DNA segment that comprises the nucleotide sequence of SEQ ID NO:10, and a DNA segment that hybridizes with the nucleic acid sequence of SEQ ID NO:2 when said nucleic acid sequence is used as a probe.
- 2. The protein of claim 1 wherein said toxin is a MIS toxin.
- 3. The protein of claim 1 wherein said toxin is a WAR toxin.
- 4. A biologically pure culture of a Bacillus laterosporus strain selected from the group consisting of MB438 and MB439.
- 5. The culture of claim 4 wherein said strain is MB438.
- 6. The culture of claim 4 wherein said strain is MB439.
- 7. An isolated polynucleotide that encodes a protein according to claim 1.
- 8. A method of controlling a corn rootworm pest wherein said method comprises contacting said pest with an isolated protein according to claim 1.
- 9. The method of claim 8 wherein said toxin is a MIS toxin.
- 10. The method of claim 8 wherein said toxin is a WAR toxin.
- 11. A plant or bacterial cell comprising an isolated polynucleotide that encodes a protein according to claim 1.
- 12. The cell of claim 11 wherein said cell is a plant cell.
- 13. The cell of claim 12 wherein said plant cell is a corn cell.
- 14. The cell of claim 13 wherein said corn cell is a corn root cell.
- 15. The cell of claim 11 wherein said cell is a bacterial cell.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The subject application is a divisional of co-pending application Ser. No. 09/967,805 (filed Sep. 28, 2001), which was a continuation of Ser. No. 09/371,913 (filed Aug. 10, 1999), now U.S. Pat. No. 6,297,369; which claims priority to Ser. No. 60/095,955 (filed Aug. 10, 1998) and to Ser. No. 60/138,251 (filed Jun. 8, 1999).
Provisional Applications (2)
|
Number |
Date |
Country |
|
60095955 |
Aug 1998 |
US |
|
60138251 |
Jun 1999 |
US |
Divisions (1)
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Number |
Date |
Country |
Parent |
09967805 |
Sep 2001 |
US |
Child |
10631407 |
Jul 2003 |
US |
Continuations (1)
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Number |
Date |
Country |
Parent |
09371913 |
Aug 1999 |
US |
Child |
09967805 |
Sep 2001 |
US |