Claims
- 1. A method for obtaining a phage particle comprising an antibody fragment directed against an antigen associated with the surface of target cells in a heterogeneous cell population, the method comprising:(a) providing a library of phage particles that express antibody fragments on the surface of the phage particles; (b) incubating the library of phage particles with said target cells under conditions that allow binding of the antibody fragment expressed on the surface of the phage particles to said antigen associated with said target cells; (c) separating said target cells and phage particles bound therewith from phage particles not bound to target cells; and (d) recovering the phage particles bound to the target cells.
- 2. A method according to claim 1, wherein the separating of said target cells and phage particles bound therewith from phage particles not bound with the target cell is accomplished by flow cytometry.
- 3. A method according to claim 1, further comprising isolating antibody fragments which bind to said target cells.
- 4. A method according to claim 2, wherein the target cells are labeled with fluorochrome-labeled antibodies.
- 5. A method according to claim 1, further comprising repeating steps (b) through (d).
- 6. A method according to claim 1, wherein the library of phage particles comprises phage particles expressing Fab or single chain Fv (scFv) antibody fragments.
- 7. A cell-type specific library of phage particles produced according to the method of claim 1.
- 8. An antibody or antibody fragment obtained using the cell-type specific library of phage particles of claim 7.
Parent Case Info
This application is a continuation-in-part of application Ser. No. 08/932,892 filed Sep. 18, 1997 (abandoned), which is a File-Wrapper-Continuation of Ser. No. 08/483,633 filed Jun. 7, 1995 (abandoned), both of which are incorporated herein by reference.
Foreign Referenced Citations (4)
Number |
Date |
Country |
0 610 774 A1 |
Aug 1994 |
EP |
WO 931123 A1 |
Jun 1993 |
WO |
WO 9426787 |
Nov 1994 |
WO |
9426787 |
Nov 1994 |
WO |
Non-Patent Literature Citations (5)
Entry |
deKruif et al PNAS 92 pages 3938-3942, 1995 Apr.* |
de Kruif et al., “Leucine Zipper Dimerized Bivalent and Bispecific scFv Antibodies from a Semi-synthetic Antibody Phage Display Library,” J. of Biological Chemistry, 271:7630-7634 (1996). |
de Kruif et al., “Rapid Selection of Cell Subpopulation-Specific Human Monoclonal Antibodies from a Synthetic Phage Antibody Library,” Proc. Natl. Acad. Sci. USA, 92:3938-3942 (1995). |
de Kruif et al., “Selection and Application of Human Single Chain Fv Antibody Fragments from a Semi-synthetic Phage Antibody Display Library with Designed CDR3 Regions,” J. Mol. Biol. 248:97-105 (1995). |
de Kruif et al., “New Perspectives on Recombinant Human Antibodies,” Immunology Today, 17:453-455 (1996). |
Continuations (1)
|
Number |
Date |
Country |
Parent |
08/483633 |
Jun 1995 |
US |
Child |
08/932892 |
|
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
08/932892 |
Sep 1997 |
US |
Child |
09/085072 |
|
US |