Patent Application
20180214381
The present invention relates to oral dosage forms for the controlled delivery of an atypical antipsychotic agent such as Paliperidone or pharmaceutical acceptable salt and a method for the preparation thereof.
Antipsychotics are the mainstay of treatment of schizophrenia. Conventional antipsychotics, typified by haloperidol, have a proven track record over the last half-century in the treatment of schizophrenia. While these drugs are highly effective against the positive, psychotic symptoms of schizophrenia, they show little benefit in alleviating negative symptoms or the cognitive impairment associated with the disease.
Second generation antipsychotics such as Paliperidone, also called atypical antipsychotics, differ considerably in their chemical, pharmacological, and clinical profiles and are generally characterized by effectiveness against both positive and negative symptoms associated with schizophrenia and by enhanced safety profile with respect to extrapyramidal symptoms.
Paliperidone also known as 9-hydroxyrisperidone is the major metabolite of risperidone. It shares the characteristic serotonin (5HT2A) and dopamine (D2) antagonism and receptor binding profile of its parent risperidone. It binds also to a1-adrenergic receptors, and, with lower affinity, to H1-histaminergic and a2-adrenergic receptors, which may explain some of the other effects of Paliperidone.
The chemical name of Paliperidone is 3-[2-[4-(6-fluoro-1,2-benzisoxazol-3-yl)-1-piperidinyl]ethyl]-6,7,8,9-tetrahydro-9-hydroxy-2-methyl-4H-pyrido[1,2-a]pyrimidin-4-one. The molecular formula is C23H27FN4O3 corresponding to a molecular weight of 426.48. It is a white to yellow color, non-hygroscopic powder. Paliperidone is sparingly soluble in dichloromethane, slightly soluble in 0.1N HCl and insoluble in water.
WO A-2007/044234 discloses an osmotic dosage form comprising a semi-permeable membrane, a first and a second orifice in the semi-permeable membrane, a controlled release drug layer, a push layer, a fast release drug layer and a barrier layer.
EP B 1539115 discloses a dosage form comprising two or more layers, said first layer comprises Paliperidone, said second layer comprises a polymer, an outer wall surrounding said two or more layers and an orifice in said outer wall.
EP B 2079446 discloses Paliperidone extended release tablet in the form of an inlay tablet comprising an inlay core comprising non-coated Paliperidone and at least one polymer capable of delaying the release of Paliperidone from the inlay core and capable of swelling upon hydration and an outer layer that partially surrounds the inlay core.
Although each of the patents above represents an attempt to provide dosage forms for the sustained delivery of Paliperidone, there still exists a need for alternative means of controlling delivery that would enhance the initial tolerability and permit initiation of treatment at an effective dose without the need for initial dose titration.
It is, therefore, an object of the present invention to provide a stable oral solid dosage formulation comprising an atypical antipsychotic agent and in particular Paliperidone or pharmaceutical acceptable salt thereof, as an active ingredient, providing a uniform and constant rate of release over an extended period of time.
It is another object of the present invention to provide a controlled release pharmaceutical composition for oral administration comprising Paliperidone as an active ingredient, which is bioavailable, with sufficient self-life and good pharmacotechnical properties.
A major object of the present invention is to provide a matrix controlling core comprising specific quantity of hydrophilic/swelling polymer and insoluble/hydrophobic polymer.
An essential object of the present invention is to choose a coating technology which in combination with matrix controlling core will allow drug release with zero order kinetics.
A further approach of the present invention is to provide a controlled release dosage form containing Paliperidone which is manufactured through a fast, simple and cost-effective process.
In accordance with the above objects of the present invention, an oral multi-layered tablet for the controlled delivery of Paliperidone is provided comprising a matrix core, an insoluble coating layer and an enteric coating layer in order to achieve zero order drug release.
According to another embodiment of the present invention, a process for the preparation of a controlled release pharmaceutical composition of Paliperidone for oral administration is provided comprising a matrix core, an insoluble coating layer and an enteric coating layer in order to achieve zero order drug release is provided, which comprises:
Other objects and advantages of the present invention will become apparent to those skilled in the art in view of the following detailed description.
For the purposes of the present invention, a pharmaceutical composition comprising an active ingredient (e.g. Paliperidone) is considered to be “stable” if said ingredient degrades less or more slowly than it does on its own and/or in known pharmaceutical compositions.
As already mentioned the main object of the present invention is to provide a controlled release composition of Paliperidone or pharmaceutical acceptable salt thereof that is simple to manufacture, bioavailable, cost effective, stable and possesses good pharmacotechnical properties.
Paliperidone exhibits polymorphism. Two polymorphs are observed, polymorph I and II, in addition to a hydrate and a solvate. Polymorph I is used in the present invention as it is the thermodynamically stable crystal form.
The development of the solid dosage form of Paliperidone SR is based on combination of matrix controlling core and coating technology.
The matrix core tablets contain both hydrophilic/swelling polymer and hydrophobic/pH independent polymer commonly used as matrix forming materials for extended release formulations.
Hydrophilic matrices are the most typical oral extended-release systems because of their ability to provide desired release profiles. Commonly used hydrophilic matrices are selected from hydroxypropylmethylcellulose (HPMC), hydroxypropylcellulose (HPC), hydroxyethylcellulose (HEC), carrageenan, sodium alginate, xanthan gum. Most preferably HPMC K100M is used in the present invention. Hydrophobic materials are also used usually in conjunction with hydrophilic systems. Commonly used hydrophobic matrices are selected from ethyl cellulose, polyvinyl alcohol, HPMC acetate succinate, methacrylic acid copolymers containing low level of quaternary ammonium groups. Most preferably Eudragit RS is used in the present invention.
It has been surprisingly found that when hydrophilic and hydrophobic polymers are incorporated together in certain quantities in the tablet matrix of the present invention zero order release can be achieved in conjunction with appropriate coating technology. More particularly, the target is achieved when 20-40% by weight of hydrophilic polymer and 5-10% by weight of hydrophobic polymer is comprised in tablet matrix of Paliperidone compositions. Most preferably 40% by weight of hydrophilic polymer and 5% by weight of hydrophobic polymer is comprised in tablet matrix of the preferred composition of the present invention.
The mechanism of drug release from hydrophilic matrix tablets is mainly based on the diffusion of the drug through the hydrated portion of the matrix as well as on the erosion of the outer hydrated polymer on the surface of the matrix. The overall drug release is affected by the rate of water uptake and the diffusion rate of the drug through the swollen gel. Incorporation of a water-insoluble hydrophobic polymer may circumvent the burst release as the former decrease the water penetration in the matrix providing appropriate drug diffusion decrease.
The drug release of the above matrix system is controlled by an insoluble coating layer of hydrophobic polymer in order to achieve zero-order drug release in small intestine and colon. Most preferably hydrophobic polymers are methacrylic acid copolymers containing high level of quaternary ammonium groups such as Eudragit RL. Such insoluble coating layer is comprised in amount of 1-2% by weight of the tablet core. Moreover, appropriate dissolution profile is achieved when an amount of 10-25% of the labelled amount of Paliperidone is contained in such insoluble coating layer.
In addition, the use of an anionic pH-dependent polymer as enteric coating layer is also necessary in order to minimize drug release in the stomach and create an initial lag phase. Most preferably, a copolymer derived from methacrylate acid/ethyl acrylate, such as Kollicoat MAE 100 is used as enteric polymer.
A separating layer is applied between tablet core and insoluble coating layer to improve stability by inhibiting any interaction between drug and insoluble coating and between insoluble coating layer and enteric layer so that dissolution of insoluble coating layer begins after dissolution of the enteric coating layer. Typical examples of substances that can be used in the separating layer include sugars, celluloses and cellulose derivatives such as hydroxypropylmethylcellulose, hydroxypropylcellulose, ethylcellulose and the like. Most preferably, hydroxypropylmethylcellulose (Opadry®) is used as separating layer in the compositions of the present invention.
By controlling the amount of each individual excipient in the formulations of the present invention, thus the extend of its contribution in sustained release properties, it is provided a final product in which the in vitro dissolution profile of Paliperidone will be segmented to achieve minimum release in the upper GI tract where solubility and permeability are high, about half of the amount released after 12 hours and maximum release over 24 hours since absorption is much decreased in the colon resulting thus in that zero order drug release.
A number of controlled release compositions comprising different excipients were tested as presented in the following examples to achieve the optimal properties with respect to the objectives of the present invention.
Compositions 1 A to 1J were prepared according to the following manufacturing process:
The tablet matrix core was prepared with HPMC K100M as hydrophilic/gelling agent and Eudragit RS as insoluble/hydrophobic polymer.
In order to select the proper ratio of these polymers different amounts were tested having as responses the % drug release at 12 h and 24 h. The target was to achieve 45-65% drug release at 12 h and more than 80% at 24 h.
The formulations were tested for their dissolution profiles in USP II apparatus, pH 6.8+0.5% tween 80, 150 rpm and data of 12 h and 24 h % drug release were used as responses to determine the proper ratio of hydrophilic and hydrophobic polymer.
According to the obtained results high amount of Eudragit RS seems to retard the drug release especially when low amount of HPMC is used.
In order to be closer to the target values of drug release after 12 h and 24 h, the amount of Eudragit RS had to be limited between 5-10% in combination with amount of HPMC between 20-40%. (Table 2)
Compositions 2A to 2C were prepared with the same process as Compositions 1A to 1J.
Compositions 2A-C were tested for their dissolution properties (USP II, pH 6.8+0.5% tween 80, 150 rpm) and were compared with trial 1E which incorporated 10% hydrophobic polymer (Eudragit RS) in the tablet matrix.
Based on the results, it was decided to maintain the amount of Eudragit RS to 5% since trials 2A and 2B exhibit the desirable drug release at 12 h and 24 h whereas amounts higher than 10% suppress the drug release.
The next step of the development was to determine the amount of insoluble coating layer that has to be applied on the tablet matrix core in order to achieve zero order drug release. (Table 3)
Using the core of trial 2A, different amounts of insoluble coating layer were applied (1%-3%) and were tested in dissolution in order to study its effect on drug release.
The insoluble polymer that was used for the coating is Eudragit RL which is also an ammonio methacrylate copolymer like Eudragit RS having 10% functional quaternary ammonium groups instead of 5% in Eudragit RS. These ammonium groups increase the pH—independent permeability of the polymers and thus films prepared with Eudragit RL are freely permeable to water, whereas films prepared with Eudragit RS are only slightly permeable to water.
Also a separating layer based on HPMC (Opadry®) was applied between the tablet core and the insoluble coating layer in order to prevent any interaction.
Compositions 3A to 3D were prepared with the same process as Compositions 1A to 1J but tablets were further coated with Opadry® (1st coating layer) and Eudragit RL containing also triethyl citrate (2nd coating layer-insoluble coating layer).
In order to study the effect of different amount of insoluble coating layer, trials 3A-D were tested in pH 6.8, 150 rpm without the use of surfactant.
The insoluble coating layer suppressed the dissolution profiles and not proper release was obtained at the target time points as well as at the initial time points.
In order to increase the dissolution profiles, mainly at early time points, it was decided to incorporate in the controlled release layer a small amount of active substance (10% of the labelled amount) so that this amount is dissolved and further absorbed in the small intestine where the permeability of Paliperidone is high. (Table 4)
Compositions 4A to 4D were prepared with the same process as Compositions 3A to 3D but in insoluble coating layer was further incorporated a small amount of active ingredient.
Dissolution data of trials 4 A-D in pH 6.8, 150 rpm showed that when amount of 3% insoluble coating layer was applied the dissolution profile was suppressed resulting in incomplete drug release over 24 hours. Amounts of up to 2% insoluble coating layer resulted in acceptable dissolution profiles since drug release was promoted at early time points.
In order to minimize the drug release in stomach where solubility and permeability of Paliperidone are maximum, an enteric coating layer (using polymer that dissolves in pH>5.5) was introduced over the insoluble coating layer.
Between the two layers a separating layer was also applied so that any interaction is prevented and erosion/dissolution of the insoluble coating layer begins after dissolution of the enteric coating layer.
Formulations based on trial 4B were prepared applying different amount of enteric coating (1-4%) as in Table 5 below.
Compositions 5A to 5D were prepared with the same process as Compositions 4A to 4D with the addition of another separating layer—Opadry® between 2nd coating layer (insoluble layer) and 4th coating layer (enteric layer) and an enteric coating layer.
The formulations were tested in dissolution methods in USP II apparatus with 150 rpm simulating fasting conditions (pH 1.2 for 2 h followed by pH 6.8 for 22 h) and fed conditions (pH 4.5 for 4 h followed by pH 6.8 for 20 h).
Comparing the dissolution profiles it can be concluded that the amount of enteric coating layer has to be up to 3% in order to achieve minimum drug release at acidic environment without decreasing the dissolution rate and extent.
Having concluded to the amount of enteric coating layer and considering the fact that the functional coating layers (enteric and insoluble layer) affect the dissolution profile at the initial time points, trials 6 and 7 were prepared with higher amount of HPMC (30% and 40% respectively) in the tablet core in order to study its effect on drug release after dissolution of the coating layers. (Table 6)
Compositions 6 and 7 were prepared with the same process as Compositions 5A to 5D.
Trials 6 and 7 were tested with USP II apparatus at 150 rpm simulating fasting conditions (pH 1.2 for 2 h followed by pH 6.8 for 22 h) and fed conditions (pH 4.5 for 4 h followed by pH 6.8 for 20 h) and the dissolution profiles were compared with trial 5B which differs only on the amount of HPMC in the tablet core (20%).
Trials 5B and 7 were also studied in vivo. Based on the in vivo data it was concluded that zero order release mechanism is achieved with both formulations exhibiting Tmax=24 h.
Additional trials were performed in which a double amount of API in the insoluble coating layer was introduced in order to increase the dissolution in the early hours (till 4-8 h) without affecting the release mechanism in vivo.
Based on tablet core of trial 7 but with 80% API in the core and the rest 20% in the insoluble coating layer, trials 7A-C were prepared applying 1.5%, 2% and 3% of insoluble coating layer respectively. (Table 7)
Compositions 7A to 7C were prepared with the same process as Compositions 5A to 5D.
Trials 7A to 7C were tested with USP II apparatus at 150 rpm simulating fasting conditions (pH 1.2 for 2 h followed by pH 6.8 for 22 h) and fed conditions (pH 4.5 for 4 h followed by pH 6.8 for 20 h) and the dissolution profiles were compared with trial 7 which differs only on the amount of active ingredient in the tablet core and the insoluble layer.
The results of dissolution testing indicate that the presence of higher amount of Paliperidone in the insoluble/controlled release layer enhances the drug release at the early hours and finally the release at 24 hours.
Also, the results confirm that the amount of controlled release layer applied on the tablet core plays a significant role on the drug release.
Trial 7A was also tested in vivo in order to study the effect of the increased amount of API in the controlled release layer. Based on the in vivo data, it is concluded that zero order drug release is achieved.
In order to study the chemical stability of trial 7A, tablets were stored at 25° C./60% RH (normal conditions) and 30° C./65% RH (intermediate conditions) for 6 months and were analyzed in terms of impurities.
According to the stability data it can be concluded that the formulation that has been developed is chemically stable after storage in normal and intermediate conditions.
While the present invention has been described with respect to the particular embodiments, it will be apparent to those skilled in the art that various changes and modifications may be made in the invention without departing from the spirit and scope thereof, as defined in the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 20150100356 | Aug 2015 | GR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2016/001164 | 7/7/2016 | WO | 00 |
