Pharmaceutical composition for preventing and treating metabolic bone diseases containing alpha-arylmethoxyacrylate derivatives

Information

  • Patent Grant
  • 8227456
  • Patent Number
    8,227,456
  • Date Filed
    Wednesday, October 14, 2009
    15 years ago
  • Date Issued
    Tuesday, July 24, 2012
    12 years ago
Abstract
The present invention relates to a use of a specific alpha-arylmethoxyacrylate derivative, or its pharmacologically acceptable salt or solvate for preventing and treating metabolic bone diseases.
Description
FIELD OF THE INVENTION

The present invention relates to a pharmaceutical composition for preventing and treating metabolic bone diseases which contains an alpha-arylmethoxyacrylate derivative, or its pharmacologically acceptable salt or solvate as an active ingredient.


DESCRIPTION OF THE PRIOR ART

Metabolic bone diseases such as osteoporosis are typically caused by reduction of protein, calcium, phosphor and others in bones. Osteoporosis occurs regardless of age and sex with increasing frequency upon aging, especially in a high frequency in postmenopausal women. Recently, the number of osteoporosis patient has been increasing due to the aging of global population and, accordingly, there has existed a need for developing an efficacious medicament for preventing and treating osteoporosis.


Currently available therapeutic agents for osteoporosis include bisphosphonates, hormonal drugs, vitamin D and its analogues, calcitonin, and calcium. Representative bisphosphonates include alendronate (Merck and Co., Ltd.), risedronate (Hoffman-La Roche Ltd.), zoledronate (Novatis AG; EP Patent No. 275,821), ibandronate (Hoffman-La Roche Ltd.; U.S. Pat. No. 4,942,157) and minodronate (Yamanouchi Pharmaceutical Co., Ltd.; EP Patent No. 354,806). Bisphosphonates are major therapeutic agents for osteoporosis; however, they have the disadvantages of low absorption rates through the gastrointestinal tract and possibility of causing esophagitis when not keeping the complicated administration guidance.


Exemplary hormonal drugs include raloxifene (Eli Lilly and Co.), droloxyfene (Pfizer Inc.; EP Patent No. 54168), lasopoxifene (Pfizer Inc.), FC-1271 (homosmedical Co. and Orion Corp., WO 96/07402), TES-424 (Ligand Co. and Weyers Co., U.S. Pat. No. 5,948,775). However, hormonal drugs have the risk of causing breast and uterine cancers and, accordingly, they are limitedly used as a therapeutic agent for osteoporosis which requires a long-term administration.


Further, vitamin D and its analogues are expensive and the therapeutic efficacy for osteoporosis thereof is not clearly established; calcitonin is relatively expensive and requires a difficult administration way; and calcium is known to cause little side effects, but is effective only for the prevention of osteoporosis, having no therapeutic effect.


SUMMARY OF THE INVENTION

Accordingly, it is a primary object of the present invention to provide a novel pharmaceutical composition for preventing and treating a metabolic bone disease having good activity and low side-effects.


In accordance with one aspect of the present invention, there is provided a pharmaceutical composition for preventing and treating metabolic bone diseases comprising a compound of formula (1), or its pharmacologically acceptable salt or solvate as an active ingredient:




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wherein,

  • A is O, S, CH2, O—N═CH or O—N═C(CH3);
  • X is H, or a halogen;
  • Y is N or CH;
  • Z is O or NH;
  • R1 is H or C1˜4 alkyl;
  • R2 is unsubstituted or substituted aryl or heteroaryl.


In accordance with another aspect of the present invention, there is provided a use of the compound of formula (1), or its pharmacologically acceptable salt or solvate for preventing and treating metabolic bone diseases.


In accordance with a further aspect of the present invention, there is provided a method for preventing and treating metabolic bone diseases using the compound of formula (1), or its pharmacologically acceptable salt or solvate.





BRIEF DESCRIPTION OF THE DRAWINGS

The above and other objects and features of the present invention will become apparent from the following description thereof, when taken in conjunction with the accompanying drawings which respectively show:



FIGS. 1
a and 1b: the changes in bone mineral density (BMD) of multiparous mice ovariectomized to induce osteoporosis observed when the mice were subcutaneously injected with compounds according to the present invention; and



FIGS. 2
a and 2b: the changes in BMD of multiparous mice ovariectomized to induce osteoporosis observed when the mice were orally administered with compounds according to the present invention.





DETAILED DESCRIPTION OF THE INVENTION

In the compound of formula (1), R2 may be an aryl group such as phenyl or naphthyl, or a 5- or 6-membered heterocyclic aromatic ring containing at least one element selected from O, S and N, such as pyridine, pyrimidie, oxazolone, 1,3,4-thiadiazole, cromene, indole, morpholine, thiomorpholine, pyrrolidine, piperidine, piperazine, N-methylpiperazine, N-acetylpiperazine, pyrrolidone, piperidone, oxazolidinone, thiazolidinone, and imidazolone.


Such an aryl or heteroaryl group represented by R2 may be substituted with at least one substituent selected from the group consisting of halogens, cyano, nitro, C1˜4 haloalkyl, C1˜4 haloalkenyl, hydroxy, C1˜8 alkyl, C2˜8 alkenyl, C2˜4 alkynyl, C3˜6 cycloalkyl, C1˜8 alkoxy, C1˜4 alkoxy C1˜4 alkyl, C3˜6 cycloalkyl C1˜4 alkyl, C1˜4 dialkoxy C1˜4 alkyl, 1,3-dioxolan-2-yl, 1,3-dioxan-2-yl, C2˜8 alkenyloxy, C2˜4 alkynyloxy, C3˜6 cycloalkyl C1˜4 alkoxy, hydroxy C1˜4 alkyl, C1˜4 acyloxy, C1˜4 alkylcarbonyl, C1˜4 alkylcarbonyloxy, C3˜6 cycloalkylcarbonyloxy, C1˜4 alkoxycarbonyl, C1˜4 dialkylamino C1˜4 alkoxy, at least one N or O-containing C2˜5 heterocyclo C1˜4 alkoxy, 2-morpholinoethoxy, 2-(piperidin-1-yl)ethoxy), unsubstituted or substituted N-containing heteroaryl, unsubstituted or substituted amino, and unsubstituted or substituted amino C1-2 alkyl.


The unsubstituted or substituted amino or amino C1-2 alkyl is represented by —(CH2)n—NR3R4, wherein n is 0, 1 or 2, R3 and R4 are each independently H, C1˜8 alkyl, C1˜8 haloalkyl, hydroxy, C2˜8 alkenyl, C2˜4 alkynyl, C3˜8 cycloalkyl, C3˜8 cycloalkyl C1˜4 alkyl, C1˜4 alkoxy C1˜4 alkyl, C3˜8 cycloalkoxy C1˜4 alkyl, C1˜8 alkylsulfonyl, at least one N, O or S-containing C2-7 heterocyclic C1˜4 alkyl, or an optionally substituted aryl; or R3 and R4 may be fused together with the nitrogen atom to which they are attached to form a heterocyclic ring.


The N-containing heteroaryl substitutent of the aryl or heteroaryl group represented by R2 may be pyrrolyl, imidazolyl, oxazolyl, thiazolyl, triazolyl, pyrazolyl, tetrazolyl, indazolyl, benzoxazolyl, benzothiazolyl, benzoimidazolyl, benzotriazolyl, isoquinolyl and quinazolyl, and it may be further substituted with at least one substituent selected from the group consisting of halogens, cyano, nitro, C1˜6 haloalkyl, C1˜6 haloalkenyl, hydroxy, C1˜8 alkyl, C2˜8 alkenyl, C2˜4 alkynyl, C3˜6 cycloalkyl, C1˜8 alkoxy, C1˜4 alkoxy C1˜4 alkyl, C3˜8 cycloalkyl C1˜4 alkyl, C1˜4 dialkoxy C1˜4 alkyl, C2˜8 alkenyloxy, C2˜4 alkynyloxy, C3˜6 cycloalkyl C1˜4 alkoxy, hydroxy C1˜4 alkyl, C1˜4 acyloxy, C1˜4 alkylcarbonyl, C1˜4 alkylcarbonyloxy, C3˜8 cycloalkylcarbonyloxy, C1˜4 alkoxycarbonyl, C1˜4 dialkylamino, and SO2NR5R6, R5 and R6 being each independently H or C1˜6 alkyl.


Representative examples of the compound of formula (1) include those shown in Tables 1a to 11, and Tables 3a to 3n later.
















TABLE 1a





Com-









pound






mp


No.
A
X
Y
Z
R1
R2
(° C.)






















1
S
H
CH
O
CH3
4-F—C6H4






2
S
H
CH
O
CH3


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3
S
H
CH
O
CH3


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4
S
H
CH
O
CH3


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141-142





5
ON═C(CH3)
H
N
O
CH3
3-HO—C6H4


6
ON═C(CH3)
H
CH
O
CH3
3-HO—C6H4


7
ON═C(CH3)
H
CH
O
CH3
3-CH2═CHCH2O—C6H4


8
ON═C(CH3)
H
CH
O
CH3
3-CH3(CH2)3O—C6H4





9
ON═C(CH3)
H
CH
O
CH3


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10
ON═C(CH3)
H
CH
O
CH3


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11
ON═C(CH3)
H
CH
O
CH3
3-(CH3)2CH(CH2)2O—C6H4


12
ON═C(CH3)
H
CH
O
CH3
3-(CH3)2C═CHCH2O—C6H4





13
ON═C(CH3)
H
CH
O
CH3


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14
ON═C(CH3)
H
CH
O
CH3
3-(CH3)2CHCH2O—C6H4


15
ON═C(CH3)
H
CH
O
CH3
3-CH3(CH2)5O—C6H4
41-42


16
ON═C(CH3)
H
CH
O
CH3
3-CH3O(CH2)2O—C6H4


17
ON═C(CH3)
H
CH
O
CH3
3-CH3O2CCH(CH3)O—C6H4


18
ON═C(CH3)
H
CH
O
CH3
3-CNCH2O—C6H4


19
ON═C(CH3)
H
CH
O
CH3
3-(CH3)2N(CH2)2O—C6H4





20
ON═C(CH3)
H
CH
O
CH3


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21
ON═C(CH3)
H
CH
O
CH3


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22
ON═C(CH3)
H
CH
O
CH3
3-CH3CO2—C6H4


23
ON═C(CH3)
H
CH
O
CH3
3-CH3(CH2)3CO2—C6H4





24
ON═C(CH3)
H
CH
O
CH3


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25
ON═CH
H
CH
O
CH3
3-CH2═CHCH2O—C6H4


26
ON═CH
H
CH
O
CH3
3-CNCH2O—C6H4


27
ON═CH
H
CH
O
CH3
3-CH3(CH2)3O—C6H4























TABLE 1b







28
ON═CH
H
CH
O
CH3


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29
ON═CH
H
CH
O
CH3
3-CH3O(CH2)2O—C6H4


30
O
H
N
O
CH3
3-CHO—C6H4


31
O
H
N
O
CH3
3-F2C═CH—C6H4


32
O
H
N
O
CH3
4-CN—C6H4


33
O
H
N
O
CH3
4-NO2—C6H4


34
O
H
N
O
CH3
3-Br—C6H4


35
O
H
N
O
CH3
4-CH3O2C—C6H4


36
O
H
N
O
CH3
3,4-Di-F—C6H3


37
O
H
N
O
CH3
4-CH3(CH2)5O—C6H4


38
O
H
N
O
CH3
4-(CH3)2CHCH2O—C6H4


39
O
H
N
O
CH3
4-CH3CH2CH(CH3)O—C6H4





40
O
H
N
O
CH3


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41
O
H
N
O
CH3
4-(CH3)2C═CHCH2O—C6H4


42
O
H
N
O
CH3
4-CH2═CH(CH2)2O—C6H4





43
O
H
N
O
CH3


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44
O
H
N
O
CH3
4-CH2═CHCH2O—C6H4


45
O
H
N
O
CH3
4-CH≡CCH2O—C6H4


46
O
H
N
O
CH3
4-CH3(CH2)3O—C6H4





47
O
H
N
O
CH3


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103-104





48
O
H
N
O
CH3


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49
O
H
N
O
CH3
4-(CH3O)2CH—C6H4


50
O
H
N
O
CH3
4-t-Bu-C6H4


51
O
H
N
O
CH3
3-CH3COCH2CH(OH)—C6H4





52
O
H
N
O
CH3


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53
O
H
N
O
CH3


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54
O
H
N
O
CH3


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55
O
H
N
O
CH3
3-F2CH—C6H4


56
O
H
N
O
CH3
3-CH3COCH2CHF—C6H4






















TABLE 1c







57
O
H
N
O
CH3
4-CHO—C6H4





58
O
H
N
O
CH3


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59
O
H
N
O
CH3
3-CH3-4-Cl—C6H3


60
O
H
N
O
CH3
3,4-Di-Cl—C6H3


61
O
H
N
O
CH3
4-F2CH—C6H4


62
O
H
N
O
CH3
2-CHO—C6H4


63
O
H
N
O
CH3
3-Cl-5-CH3O—C6H3


64
O
H
N
O
CH3
3-(CH3O)2CH—C6H4


65
O
H
N
O
CH3
2-F2CH—C6H4


66
O
H
N
O
CH3
2-(CH3O)2CH—C6H4





67
O
H
N
O
CH3


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68
O
H
N
O
CH3


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69
O
H
N
O
CH3
C6H5


70
O
H
N
O
CH3
2-CH3—C6H4


71
O
H
N
O
CH3
4-CH3—C6H4


72
O
H
N
O
CH3
3-Cl—C6H4


73
O
H
N
O
CH3
4-Cl—C6H4


74
O
H
N
O
CH3
3-F—C6H4


75
O
H
N
O
CH3
4-F—C6H4


76
O
H
N
O
CH3
3-CH3OC—C6H4


77
O
H
CH
O
CH2CH3
4-CH3—C6H4


78
O
H
CH
O
CH2CH3
3-Cl—C6H4


79
O
H
CH
O
CH2CH3
4-F—C6H4


80
O
H
CH
O
CH2CH3
4-Br—C6H4


81
O
H
CH
O
CH2CH3
4-t-Bu-C6H4





82
O
H
CH
O
CH2CH3


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83
O
H
CH
O
CH2CH3
3-(CH3O)2CH—C6H4


84
O
H
CH
O
CH2CH3
3-HOCH2—C6H4





85
O
H
CH
O
CH2CH3


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86
O
H
CH
O
CH2CH3
3-CH3(CH2)5O—C6H4


87
O
H
CH
O
CH2CH3
3-(CH3)2CHCH2O—C6H4


88
O
H
CH
O
CH2CH3
3-CH3O2CCH(CH3)O—C6H4


89
O
H
CH
O
CH2CH3
3-CH2═CHCH2O—C6H4























TABLE 1d







90
O
H
CH
O
CH(CH3)2


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91
O
H
CH
O
CH(CH3)2


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92
O
H
CH
O
CH(CH3)2
3-HOCH2—C6H4


93
O
H
CH
O
CH(CH3)2
3-F2CH—C6H4


94
O
H
CH
O
CH(CH3)2
3-(CH3O)2CH—C6H4


95
O
H
CH
O
CH(CH3)2
4-CH3—C6H4


96
O
H
CH
O
CH(CH3)2
3-Cl—C6H4


97
O
H
CH
O
CH(CH3)2
4-F—C6H4


98
O
H
CH
O
CH(CH3)2
3-Br—C6H4


99
O
H
CH
O
CH(CH3)2
4-t-Bu-C6H4





100
O
H
CH
O
CH(CH3)2


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101
O
H
CH
O
CH(CH3)2
3-CH3O(CH2)2O—C6H4


102
O
H
CH
O
CH(CH3)2
3-CH3(CH2)5O—C6H4


103
O
H
CH
O
CH(CH3)2
3-(CH3)2CHCH2O—C6H4


104
O
H
CH
O
CH(CH3)2
3-CH3CH2CH(CH3)O—C6H4


105
O
H
CH
O
CH(CH3)2
3-(CH3)2C═CHCH2O—C6H4


106
O
H
CH
O
CH(CH3)2
3-CH3(CH2)3O—C6H4


107
O
H
CH
O
CH(CH3)2
3-CH2═CH(CH2)2O—C6H4


108
O
H
CH
O
CH(CH3)2
3-CH3O2CCH(CH3)O—C6H4


109
O
H
CH
O
CH(CH3)2
4-CH2═CHCH2O—C6H4


110
O
H
CH
O
CH(CH3)2
4-CH≡CCH2O—C6H4





111
O
H
CH
O
CH(CH3)2


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112
O
H
CH
O
CH3
3-C6H5CO2—C6H4
121-122


113
O
H
CH
O
CH3
3-HO—C6H4


114
O
H
CH
O
CH3
3-CH3COCH2CH(OH)—C6H4


115
O
H
CH
O
CH3
3-CH3COCH═CH—C6H4
97-98


116
O
H
CH
O
CH3
3-CHO—C6H4


117
O
H
CH
O
CH3
3-F2C═CH—C6H4


118
O
H
CH
O
CH3
4-CF3CO—C6H4


119
O
H
CH
O
CH3
4-CF3CH(OH)—C6H4


120
O
H
CH
O
CH3
4-CF3CH(Cl)—C6H4


121
O
H
CH
O
CH3
4-CHO—C6H4
84-85


122
O
H
CH
O
CH3
2-CHO—C6H4


123
O
H
CH
O
CH3
2-F2CH—C6H4


124
O
H
CH
O
CH3
C6H5


125
O
H
CH
O
CH3
2-CH3—C6H4


126
O
H
CH
O
CH3
4-CH3—C6H4


127
O
H
CH
O
CH3
3-Cl—C6H4


128
O
H
CH
O
CH3
4-Cl—C6H4


129
O
H
CH
O
CH3
3-F—C6H4


130
O
H
CH
O
CH3
4-F—C6H4























TABLE 1e







131
O
H
CH
O
CH3
3-CH3OC—C6H4
76-77


132
O
H
CH
O
CH3
4-CH3O2C—C6H4


133
O
H
CH
O
CH3
3-(CH3O)2CH—C6H4


134
O
H
CH
O
CH3
4-CN—C6H4


135
O
H
CH
O
CH3
4-NO2—C6H4


136
O
H
CH
O
CH3
3-Br—C6H4


137
O
H
CH
O
CH3
3,4-Di-F—C6H3


138
O
H
CH
O
CH3
3-CH3—C6H4


139
O
H
CH
O
CH3
2-Cl—C6H4


140
O
H
CH
O
CH3
2-F—C6H4


141
O
H
CH
O
CH3
2,4-Di-Cl—C6H3


142
O
H
CH
O
CH3
2,5-Di-Cl—C6H3


143
O
H
CH
O
CH3
2,6-Di-Cl—C6H3


144
O
H
CH
O
CH3
3,5-Di-Cl—C6H3


145
O
H
CH
O
CH3
2,4-Di-t-Bu-C6H3


146
O
H
CH
O
CH3
2,6-Di-F—C6H3


147
O
H
CH
O
CH3
2,4,6-Tri-Cl—C6H2


148
O
H
CH
O
CH3
2,4,5-Tri-Cl—C6H2


149
O
H
CH
O
CH3
2,-CH3-4-Cl—C6H3


150
O
H
CH
O
CH3
3,5-Di-CH3-4-Cl—C6H2


151
O
H
CH
O
CH3
3-CH3O—C6H4


152
O
H
CH
O
CH3
2-CN—C6H4


153
O
H
CH
O
CH3
3-CN—C6H4


154
O
H
CH
O
CH3
4-F2C═CH—C6H4
56-57


155
O
H
CH
O
CH3
4-CH3O2CCH2—C6H4


156
O
H
CH
O
CH3
3-CH3CH(OH)—C6H4





157
O
H
CH
O
CH3


embedded image







158
O
H
CH
O
CH3


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159
O
H
CH
O
CH3


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160
O
H
CH
O
CH3


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161
O
H
CH
O
CH3


embedded image

























TABLE 1f







162
O
H
CH
O
CH3
4-F2CH—C6H4



163
O
H
CH
O
CH3
4-(CH3O)2CH—C6H4


164
O
H
CH
O
CH3
2-CH3CH(OH)—C6H4


165
O
H
CH
O
CH3
4-CH3CH(OH)—C6H4


166
O
H
CH
O
CH3
2-HOCH2—C6H4


167
O
H
CH
O
CH3
4-HOCH2—C6H4


168
O
H
CH
O
CH3
2-CH3CH(F)—C6H4


169
O
H
CH
O
CH3
3-CH3CH(F)—C6H4


170
O
H
CH
O
CH3
4-CH3CH(F)—C6H4


171
O
H
CH
O
CH3
4-CH3O(CH2)2O—C6H4





172
O
H
CH
O
CH3


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173
O
H
CH
O
CH3
4-CH3(CH2)5O—C6H4


174
O
H
CH
O
CH3
4-(CH3)2CHCH2O—C6H4


175
O
H
CH
O
CH3
4-CH3CH2CH(CH3)O—C6H4


176
O
H
CH
O
CH3
4-(CH3)2C═CHCH2O—C6H4


177
O
H
CH
O
CH3
4-CH3(CH2)3O—C6H4


178
O
H
CH
O
CH3
4-CH2═CH(CH2)2O—C6H4


179
O
H
CH
O
CH3
4-CH2═CHCH2O—C6H4
74-75


180
O
H
CH
O
CH3
4-CH3O2CCH(CH3)O—C6H4


181
O
H
CH
O
CH3
3-CH3O2CCH(CH3)O—C6H4


182
O
H
CH
O
CH3
2-CH3CH2O—C6H4


183
O
H
CH
O
CH3
4-CH3CH2O—C6H4
77-78


184
O
H
CH
O
CH3
2,6-Di-CH3O—C6H3


185
O
H
CH
O
CH3
3,5-Di-CH3O—C6H3


186
O
H
CH
O
CH3
3,4-Di-CH3O—C6H3


187
O
H
CH
O
CH3
2,3-Di-CH3O—C6H3


188
O
H
CH
O
CH3
3,4,5-tri-CH3O—C6H2


189
O
H
CH
O
CH3
4-t-Bu-C6H4


190
O
H
CH
O
CH3
3-FCH2—C6H4


191
O
H
CH
O
CH3
4-PhCH2O—C6H4


192
O
H
CH
O
CH3
4-CH3(CH2)2O—C6H4


193
O
H
CH
O
CH3
4-CH3(CH2)4O—C6H4


194
O
H
CH
O
CH3
4-CH3(CH2)6O—C6H4


195
O
H
CH
O
CH3
4-CH3(CH2)7O—C6H4


196
O
H
CH
O
CH3
4-CH2═CH(CH2)6O—C6H4


197
O
H
CH
O
CH3
3-CH2═CH(CH2)6O—C6H4


198
O
H
CH
O
CH3
3-CH3CH2O—C6H4


199
O
H
CH
O
CH3
3-CH3(CH2)2O—C6H4


200
O
H
CH
O
CH3
3-CH3(CH2)4O—C6H4


201
O
H
CH
O
CH3
3-CH3(CH2)6O—C6H4


202
O
H
CH
O
CH3
3-CH3(CH2)7O—C6H4


203
O
H
CH
O
CH3
4-CH3CO(CH2)2—C6H4


204
O
H
CH
O
CH3
4-CH3COCH═CH—C6H4























TABLE 1g







205
O
H
CH
O
CH3
3-CH3CF2CH2CHF—C6H4



206
O
H
CH
O
CH3
3-CH3COCH2CHF—C6H4


207
O
H
CH
O
CH3
3-F2CH—C6H4


208
O
H
CH
O
CH3
3-(CN)2C═CH—C6H4
112-113





209
O
H
CH
O
CH3


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210
O
H
CH
O
CH3


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211
O
H
CH
O
CH3
3-CH2═CHCH2—C6H4


212
O
H
CH
O
CH3
3-CH3(CH2)3O—C6H4


213
O
H
CH
O
CH3
3-CNCH2O—C6H4


214
O
H
CH
O
CH3
3-CH2═CH(CH2)2O—C6H4





215
O
H
CH
O
CH3


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216
O
H
CH
O
CH3
3-(CH3)2CH(CH2)2O—C6H4


217
O
H
CH
O
CH3
3-(CH3)2C═CHCH2O—C6H4





218
O
H
CH
O
CH3


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219
O
H
CH
O
CH3
3-(CH3)2CHCH2O—C6H4


220
O
H
CH
O
CH3
3-CH3(CH2)5O—C6H4


221
O
H
CH
O
CH3
3-CH3CH2CH(CH3)O—C6H4





222
O
H
CH
O
CH3


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223
O
H
CH
O
CH3
3-CH3O(CH2)2O—C6H4


224
O
H
CH
O
CH3
3-(CH3CH2O)2CH—C6H4


225
O
H
CH
O
CH3
3-(CH3CH2CH2CH2O)2CH—C6H4


226
O
H
CH
O
CH3
3-Cl-5-CH3O—C6H3


227
O
H
CH
O
CH3
3,4-Di-Cl—C6H3


228
O
H
CH
O
CH3
3-CH3-4-Cl—C6H3


229
O
H
CH
O
CH3
3-CH3(CH2)7—C6H4


230
O
H
CH
O
CH3
4-CH3(CH2)7—C6H4


231
O
H
CH
O
CH3
3-CH3CO2—C6H4


232
O
H
CH
O
CH3
3-CH3(CH2)2CO2—C6H4





233
O
H
CH
O
CH3


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TABLE 1h







234
O
H
CH
O
CH3


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235
O
H
CH
O
CH3


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236
O
H
CH
O
CH3
3-(CH3)2N(CH2)2O—C6H4





237
O
H
CH
O
CH3


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238
O
H
CH
O
CH3


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239
O
H
CH
O
CH3


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240
O
H
CH
O
CH3


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241
O
H
CH
O
CH3


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242
O
H
CH
O
CH3


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243
O
H
CH
O
CH3


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244
O
H
CH
O
CH3


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245
O
H
CH
O
CH3


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246
O
H
CH
O
CH3


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247
O
H
CH
O
CH3


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248
O
H
CH
O
CH3


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249
O
H
CH
O
CH3


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110-111






















TABLE 1i







250
O
H
CH
O
CH3


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251
O
H
CH
O
CH3


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252
O
H
CH
O
CH3


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253
O
H
CH
O
CH3


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254
O
H
CH
O
CH3


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255
O
H
CH
O
CH3


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256
O
H
CH
O
CH3


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257
O
H
CH
O
CH3


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258
O
H
CH
O
CH3


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259
O
4-Cl
CH
O
CH3


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260
O
4-Cl
CH
O
CH3


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261
O
4-Cl
CH
O
CH3
3-F2CH—C6H4


262
O
4-Cl
CH
O
CH3
3-HOCH2—C6H4


263
O
4-Cl
CH
O
CH3
3-FCH2—C6H4


264
O
4-Cl
CH
O
CH3
4-CH2═CHCH2O—C6H4


265
O
4-Cl
CH
O
CH3
4-CH3(CH2)3O—C6H4


266
O
4-Cl
CH
O
CH3
4-CH2═CH(CH2)2O—C6H4





267
O
4-Cl
CH
O
CH3


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268
O
4-Cl
CH
O
CH3
4-(CH3)2CH(CH2)2O—C6H4


269
O
4-Cl
CH
O
CH3
4-(CH3)2C═CHCH2O—C6H4






















TABLE 1j







270
O
4-Cl
CH
O
CH3
4-(CH3)2CHCH2O—C6H4


271
O
4-Cl
CH
O
CH3
4-CH3(CH2)5O—C6H4


272
O
4-Cl
CH
O
CH3
4-CH3CH2CH(CH3)O—C6H4





273
O
4-Cl
CH
O
CH3


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274
O
4-Cl
CH
O
CH3
4-CH3O(CH2)2O—C6H4





275
O
4-Cl
CH
O
CH3


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276
O
4-Cl
CH
O
CH3


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277
O
3-F
CH
O
CH3
3-CH2═CHCH2O—C6H4


278
O
3-F
CH
O
CH3
3-CH3(CH2)3O—C6H4





279
O
3-F
CH
O
CH3


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280
O
3-F
CH
O
CH3


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281
O
3-F
CH
O
CH3
3-(CH3)2CH(CH2)2O—C6H4


282
O
3-F
CH
O
CH3
3-(CH3)2C═CHCH2O—C6H4





283
O
3-F
CH
O
CH3


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284
O
3-F
CH
O
CH3
3-(CH3)2CHCH2O—C6H4


285
O
3-F
CH
O
CH3
3-CH3(CH2)5O—C6H4


286
O
3-F
CH
O
CH3
3-CH3CH2CH(CH3)O—C6H4





287
O
3-F
CH
O
CH3


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288
O
3-F
CH
O
CH3
3-CH3CH═CHCH2O—C6H4


289
O
3-F
CH
O
CH3
4-CH2═CHCH2O—C6H4


290
O
3-F
CH
O
CH3
4-CH3(CH2)3O—C6H4





291
O
3-F
CH
O
CH3


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292
O
3-F
CH
O
CH3


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293
O
3-F
CH
O
CH3
4-(CH3)2CH(CH2)2O—C6H4





294
O
3-F
CH
O
CH3


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295
O
3-F
CH
O
CH3
4-(CH3)2CHCH2O—C6H4


296
O
3-F
CH
O
CH3
4-CH3(CH2)5O—C6H4


297
O
3-F
CH
O
CH3
4-CH3CH2CH(CH3)O—C6H4






















TABLE 1k







298
O
3-F
CH
O
CH3
4-CH3O(CH2)2O—C6H4


299
O
3-F
CH
O
CH3
4-CH3CH═CHCH2O—C6H4





300
O
3-F
CH
O
CH3


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301
O
3-F
CH
O
CH3


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302
O
3-F
CH
O
CH3
3-(CH3O)2CH—C6H4


303
O
3-F
CH
O
CH3
3-HOCH2—C6H4


304
O
3-F
CH
O
CH3
3-FCH2—C6H4


305
O
4-F
CH
O
CH3
3-CH3O(CH2)2O—C6H4


306
O
4-F
CH
O
CH3
4-CH3O(CH2)2O—C6H4


307
O
4-F
CH
O
CH3
3-CH2═CHCH2O—C6H4


308
O
4-F
CH
O
CH3
4-CH2═CHCH2O—C6H4





309
O
4-F
CH
O
CH3


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310
O
4-F
CH
O
CH3
3-(CH3)2CH(CH2)2O—C6H4


311
O
4-F
CH
O
CH3
3-CH3(CH2)3O—C6H4


312
O
4-F
CH
O
CH3
3-CH3(CH2)5O—C6H4





313
O
4-F
CH
O
CH3


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314
O
4-F
CH
O
CH3
3-(CH3)2C═CHCH2O—C6H4





315
O
4-F
CH
O
CH3


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316
O
4-F
CH
O
CH3
4-(CH3)2CH(CH2)2O—C6H4


317
O
4-F
CH
O
CH3
4-CH3(CH2)3O—C6H4





318
O
4-F
CH
O
CH3


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319
O
4-F
CH
O
CH3
4-CH3CH2CH(CH3)O—C6H4





320
O
4-F
CH
O
CH3


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321
CH2
H
CH
O
CH3
3-HO—C6H4


322
CH2
H
CH
O
CH3
3-CH3(CH2)2O—C6H4


323
CH2
H
CH
O
CH3
3-CH3(CH2)3O—C6H4


324
CH2
H
CH
O
CH3
3-CH3(CH2)4O—C6H4


325
CH2
H
CH
O
CH3
3-CH3(CH2)5O—C6H4


326
CH2
H
CH
O
CH3
3-CH3(CH2)6O—C6H4


327
CH2
H
CH
O
CH3
3-CH3(CH2)7O—C6H4


328
CH2
H
CH
O
CH3
3-(CH3)2CH(CH2)2O—C6H4






















TABLE 11







329
CH2
H
CH
O
CH3
3-(CH3)2CHCH2O—C6H4


330
CH2
H
CH
O
CH3
3-CH3CH2CH(CH3)O—C6H4


331
CH2
H
CH
O
CH3
3-CH2═CHCH2O—C6H4


332
CH2
H
CH
O
CH3
3-(CH3)2C═CHCH2O—C6H4





333
CH2
H
CH
O
CH3


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334
CH2
H
CH
O
CH3
3-CH3O(CH2)2O—C6H4





335
CH2
H
CH
O
CH3


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336
CH2
H
CH
O
CH3
4-CH2═CHCH2O—C6H4


337
CH2
H
CH
O
CH3
4-CH3(CH2)3O—C6H4





338
CH2
H
CH
O
CH3


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339
CH2
H
CH
O
CH3


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340
CH2
H
CH
O
CH3
4-(CH3)2CH(CH2)2O—C6H4


341
CH2
H
CH
O
CH3
4-(CH3)2C═CHCH2O—C6H4





342
CH2
H
CH
O
CH3


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343
CH2
H
CH
O
CH3
4-(CH3)2CHCH2O—C6H4


344
CH2
H
CH
O
CH3
4-CH3(CH2)5O—C6H4


345
CH2
H
CH
O
CH3
4-CH3CH2CH(CH3)O—C6H4





346
CH2
H
CH
O
CH3


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Among the compounds of formula (1), preferred are those wherein A is O or O—N═C(CH3); X is H, F or Cl, Y is CH, Z is O, R1 is methyl, and R2 is substituted or unsubstituted aryl.


Particularly preferred are those of formula (1a):




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wherein,

  • A is O or O—N═C(CH3);
  • X represents H, F or Cl;
  • Ab is at least one group selected from the group consisting of halogens, C1˜4 haloalkyl, C1˜4 haloalkenyl, C1˜8 alkyl, C2˜8 alkenyl, C2˜4 alkynyl, C3˜6 cycloalkyl, C1˜8 alkoxy, C1˜4 alkoxy C1˜4 alkyl, C3˜6 cycloalkyl C1˜4 alkyl, C1˜4 dialkoxy C1˜4 alkyl, 1,3-dioxolan-2-yl, 1,3-dioxan-2-yl, C2˜8 alkenyloxy, C2˜4 alkynyloxy, C3˜6 cycloalkyl C1˜4 alkoxy, hydroxy C1˜4 alkyl, C1˜4 dialkylamino C1˜4 alkoxy, at least one N or O-containing C2˜5 heterocyclo C1˜4 alkoxy, 2-morpholinoethoxy, 2-(piperidin-1-yl)ethoxy), unsubstituted or substituted N-containing heteroaryl, unsubstituted or substituted amino, and unsubstituted or substituted amino C1-2 alkyl.


Specific examples of the compounds of formula (1) include:

  • (E)-methyl 2-(2-((4-octylphenoxy)methyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(cyclopropylmethoxy)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(2-methoxyethoxy)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(allyloxy)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(2-methoxyethoxy)phenoxy)methyl)-4-fluorophenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(allyloxy)phenoxy)methyl)-4-fluorophenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(1-methylpropaneoxy)phenoxy)methyl)-4-fluorophenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-(2-morpholinoethoxy)phenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-(1,3-dioxan-2-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(allyloxy)phenethyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(((({1E}-1-(3-(n-hexyloxy)phenyl)ethylidene)amino)oxy)methyl)phenyl-3-methoxyacrylate;
  • (E)-methyl 2-(((({1E}-1-(3-(n-cyanomethyloxy)phenyl)ethylidene)amino)oxy)methyl)phenyl-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-morpholinophenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-morpholinophenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-(piperidin-1-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(piperidin-1-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-(4-methylpiperizan-1-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(N-isobutylamino)-2-fluorophenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(N-isobutyl-N-methylamino)-2-fluorophenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((4-(N-cyclopropylmethylamino)-2-fluorophenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-(4-(N-cyclopropylmethyl-N-methylamino)-2-fluorophenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-fluoro-4-(piperidin-1-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((2-fluoro-4-morpholinophenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-(morpholinomethyl)phenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-(N-methyl-N-phenylamino)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((3-((4-methylpiperizan-1-yl)methyl)phenoxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((6-(pyrrolidin-1-yl)pyridin-2-yloxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((6-(piperidin-1-yl)pyridin-2-yloxy)methyl)phenyl)-3-methoxyacrylate;
  • (E)-methyl 2-(2-((5-(morpholino)pyridin-2-yloxy)methyl)phenyl)-3-methoxyacrylate; and
  • (E)-methyl 2-(2-((6-(morpholino)pyridin-2-yloxy)methyl)phenyl)-3-methoxyacrylate.


The inventive composition may also comprise physiologically and pharmaceutically acceptable salts of the compound of formula (1) as active ingredients. The pharmaceutically acceptable salts may be non-toxic and water-soluble salts. Representative examples thereof include alkali metal salts such as sodium and potassium salts; alkaline earth metal salts such as magnesium and calcium salts; ammonium salts such as tetramethylammonium salts; amine salts such as triethylamine, methylamine, dimethylamine, cyclopentylamine, benzylamine, phenethylamine, piperidine, monoethanolamine, diethanolamine, tris(hydroxymethyl)-aminomethane, lysine, arginine and N-methyl-D-glucarmine salts; inorganic acid salts such as hydrochloric, hydrobromic, hydroiodic, sulfuric, phosphoric and nitric acid salts; organic acid salts such as acetic, lactic, tartaric, benzoic, citric, methanesulfonic, ethanesulfonic, benzenesulfonic, toluenesulfonic, isetionic, glucuronic and gluconic acid salts; hydrates; and solvates such as alcoholates (e.g., ethanolate).


The compound of formula (1) used in the present invention may be prepared by the method described in European Patent Publication No. 278,595. For example, a compound of formula (1a) may be prepared by reacting a compound of formula (2) with a compound of formula (3) in the presence of a base.




embedded image




embedded image



wherein, X, Y, A and Ab have the same meanings as defined above.


Further, the compound of formula (2) used as the starting material in the above reaction may be prepared as shown in the Reaction Scheme 1:




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wherein, X has the same meaning as defined above; and Q is a halogen such as iodine, bromine and chorine.


As shown in Reaction Scheme 1, the compound of formula (2) may be prepared by the method comprising the steps of: reacting the aryl halide compound of formula (4) (preferably, Q is iodine or bromine) with triisopropylboronate in the presence of a base such as n-butyl lithium, and treating the resulting mixture with an acidic solution, e.g., hydrochloric acid, to form the compound of formula (5) (see W Li et al., J. Org. Chem., 67, 5394, 2002); reacting the compound of formula (5) with methyl α-halomethoxyacrylate of formula (6) (preferably, Q is iodine or bromine) which is prepared from methyl propionate as a starting material according to the methods disclosed in R. E. Ireland et al., J. Org. Chem., 56, 3572, 1991 and D. M. Hodgson et al., Synlett, 32, 1995, in the presence of a palladium catalyst, e.g., Pd(OAc)2 or Pd(PPh3)4 and an inorganic salt, e.g., K2CO3, Na2CO3, K2PO4 or Cs2CO3, to form the compound of formula (7); and treating the compound of formula (7) with N-bromosuccinimide.


A particular compound of formula (2), (E)-methyl-2-(2-bromomethylphenyl)-3-methoxyacrylate (i.e., the compound of formula (2) wherein X is hydrogen) may be prepared by the method disclosed in European Patent Publication No. 278,595.


The compounds of formula (3) may be also prepared in a conventional manner, and those having O—N═C(CH3) as the A group in particular may be obtained by the method disclosed in Korean Patent Nos. 31195 and 311 96, and those wherein A is oxygen and Ab is amino, by the method disclosed in Hassen, J. et al., Chemical Review, 102, 1359, 2002.


In addition, the compound of formula (1a) may be prepared according to the procedure shown in Reaction Scheme 2:




embedded image



wherein, X, Ab and Q have the same meanings as defined above.


In accordance with Reaction Scheme 2, the compound of formula (1a) may be prepared by the method comprising the steps of: treating the halotoluene compound of formula (8) (preferably, Q is iodine or bromine) with N-bromosuccinimide to form the benzylbromide compound of formula (9); reacting the compound of formula (9) with the phenol compound of formula (3a) to form the compound of formula (10); allowing the compound of formula (10) to react with triisopropylboronate in the presence of a base, e.g., n-butyl lithium and treating the resulting mixture with an acidic solution, e.g., hydrochloric acid, to form the compound of formula (11); and reacting the compound of formula (11) with the compound of formula (6) in the presence of a palladium catalyst, e.g., Pd(OAc)2 and Pd(PPh3)4 and an inorganic salt, e.g., K2CO3, Na2CO3, K2PO4 and Cs2CO3.


The compound of formula (3a) used in the above method may be prepared by a known method, that having an amino group for Ab may be synthesized by the method disclosed in Hassen, J. et al., Chemical Review, 102, 1359, 2002 and Wolfe, J. P. et al., J. Org. Chem., 65, 1158, 2000, and that having —NR3R4 for Ab (the compound of formula (3a-1)) may be prepared according to the procedure shown in Reaction Scheme 3.




embedded image



wherein, R3 and R4 have the same meanings as defined above; L is a halogen or OSO2CF3; and PG is methyl, benzyl or trialkylsilyl (e.g., trimethylsilyl, triethylsilyl, triisopropylsilyl or t-butyldimethylsilyl).


That is, the compound of formula (3a-1) may be prepared by amination of the compound of formula (12), followed by deprotection of the compound of formula (13) obtained from the amination.


The amination of the compound of formula (12) may be carried out by a conventional amination method (see Smith, M. B. et al., Advanced Organic Chemistry, 5th Ed., pp 850-893, 2001) and the deprotection may be carried out by a conventional deprotection method (see Greene, T. W. et al., Protective Groups in Organic Synthesis, 3rd Ed., pp 23-148, 1999). The amination may be carried out in an inert solvent in the presence of a palladium catalyst, a base and a phosphine ligand. Exemplary palladium catalysts include, but are not limited to, palladium (II) acetate, palladium (II) chloride, palladium (II) bromide, dichlorobis(triphenylphosphine) palladium (II), tetrakis(triphenylphosphine) palladium(0) and tris(dibenzylidene acetone) dipalladium(0). Exemplary phosphine ligands include, but are not limited to, 2,2′-bis(diphenylphosphino)-1,1′-binaphthyl (BINAP), tri-o-tolylphosphine, tri-t-butylphosphine, 1,1′-bis(diphenylphosphino) ferrocene, bis[(2-diphenylphosphino)-phenyl]ether (DPEphos), 2-dicyclohexylphosphanyl-2′-dimethylaminobiphenyl, 2-(di-t-butylphosphino) biphenyl, 9,9′-dimethyl-4,6-bis(diphenylphosphino) xanthene (Xanthaphos) and a racemate thereof. Exemplary bases include sodium t-butoxide (t-BuONa) and an inorganic salt (e.g., K2CO3, Na2CO3, K2PO4 or Cs2CO3). Exemplary inert solvents include 1,4-dioxane, toluene, benzene, acetonitrile, dimethylformamide and tetrahydrofuran. The palladium catalyst and the phosphine ligand may be used in catalytic amounts, preferably in amounts ranging from 0.1 to 10% by mol based on the compound of formula 12. The amination may be carried out at 80 to 150° C. for 3 to 30 minutes under an inert gas such as argon or nitrogen.


As also shown in Reaction Scheme 3, the compound of formula 13 may be prepared by reduction of a nitro group-containing compound of formula 14, followed by an alkylation of the resulting amino-containing compound, i.e., the compound of formula 15.


Further, the compound of formula (3a) having a substituted aminomethyl group (—CH2—NR3R4) for Ab (the compound of formula (3a-2)) may be prepared by a method as shown in Reaction Scheme 4.




embedded image



wherein, R3 and R4 have the same meanings as defined above.


That is, the compound of formula (3a-2) may be prepared by amination of the aldehyde compound of formula (16) in a conventional manner. The amination of Reaction Scheme 4 may be carried out in an inert solvent in the presence of a reducing agent. Exemplary reducing agents include, but are not limited to, sodium borohydride (NaBH4), sodium cyanoborohydride (NaBH3CN) and sodium triacetoxyborohydride (NaBH(OAc)3).


The compound of formula (1) is used in a pharmaceutical composition for treating or preventing metabolic bone diseases, as an active ingredient, together with pharmaceutically acceptable carriers. Exemplary pharmaceutically acceptable carriers include excipients, disintegrants, sweeting agents, lubricants and flavoring agents. The inventive composition may further comprise other components such as vitamin C for enhancing health, if necessary.


The pharmaceutical composition of the present invention may be formulated in various forms such as a tablet, capsule, powder, granule, and solution such as suspension, emulsion and syrup, and other forms for oral or parenteral administration. The inventive pharmaceutical composition may be administrated in a single dose or in divided doses. In case of the parenteral administration, a typical daily dose of the active ingredient ranges from 0.5 to 5 mg/kg of body weight, preferably 1 to 4 mg/kg of body weight, and in case of oral administration, 5 to 50 mg/kg of body weight, preferably 10 to 40 mg/kg of body weight. However, it should be understood that the amount of the active ingredient actually administered ought to be determined in light of various relevant factors including the condition to be treated, the chosen route of administration, the age, sex and body weight of the individual patient, and the severity of the patient's symptom; and, therefore, the above dose should not be intended to limit the scope of the invention in any way.


In accordance with another aspect of the present invention, there is provided a healthy food or drink composition for treating or preventing metabolic bone diseases such as osteoporosis, comprising the compound of formula (1) as an active ingredient. Exemplary foods and drinks to which the compound of formula (1) may be applied include, but are not limited to, meats, beverages, chocolates, snacks, confectionery, pizza, instant noodles, various noodles, gums, ice creams, alcoholic beverages, and vitamin formulations. In the healthy food or drink composition, the compound of formula (1) may be employed in an amount ranging from 0.1 to 80% by weight of the composition.


The present invention will be described in further detail with reference to Examples. However, it should be understood that the present is not restricted by the specific Examples.


EXAMPLE 1
Preparation of (E)-methyl-2-(2-((4-(cyclopropylmethyl)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate (Compound No. 267)

Step 1)


13.3 ml (0.1 mol) of 2-bromo-5-chlorotoluene was dissolved in 200 ml of anhydrous THF, and 27.7 ml (0.12 mol) of triisopropyl borate was added thereto. After cooling the reaction mixture to −78° C. over a dry ice-acetone bath, 48 ml (0.12 mol) of 2.5 M n-BuLi (in hexane) was added dropwise thereto for 1 hour, the dry ice-acetone bath was removed, and 150 ml of 3 N HCl was added thereto. The resulting mixture was stirred for 1 hour, and the separated water layer was extracted twice with 100 ml portions of ethyl acetate. The organic layers were combined, washed with a brine solution, dried over anhydrous MgSO4, and filtered under a reduced pressure to remove the solvent. The residue was recrystallized from 10% ethyacetate/hexane to obtain 13.8 g (yield 81%) of 4-chloro-2-methylboronic acid as a white solid.



1H NMR (300 MHz, CDCl3) d 8.07 (dd, 1H, J=5.7 Hz, 2.8 Hz), 7.28-7.26 (m, 2H), 2.76 (s, 3H)


Step 2)


13.8 g (80 mmol) of the compound obtained in Step 1, 3.1 g (2.7 mmol) of tetrakis(triphenylphosphine) palladium and 42.7 g (200 mmol) of K3PO4 were placed successively in a flask, and 450 ml of dioxane and 90 ml of water were added thereto. After adding 16.2 g (67 mmol) of (E)-methyl-2-iodo-3-methoxy-2-propenoate thereto, the mixture was stirred at 90° C. for 22 hours and cooled to room temperature, and 200 ml of ethyl acetate was added thereto. The separated water layer was extracted twice with 50 ml portions of ethyl acetate, and the organic layers were combined, washed with 100 ml of water and 100 ml of a brine solution. The resulting solution was dried over anhydrous MgSO4, and concentrated under a reduced pressure. The concentrate was subjected to column chromatography using a mixture of 10% ethyl acetate/hexane as an eluent to obtain 12.6 g (yield 78%) of (E)-methyl-2-(4-chloro-2-methylphenyl)-3-methoxyacrylate as a solid.



1H NMR (300 MHz, CDCl3) d 7.56 (s, 1H), 7.22-7.14 (m, 2H), 7.03 (d, 1H, J=8.2 Hz), 3.83 (s, 3H), 3.70 (s, 3H), 2.15 (s, 3H)


Step 3)


9.7 g (40 mmol) of the compound obtained in Step 2 was dissolved in 200 ml of carbon tetrachloride, and 0.7 g (4 mmol) of AIBN and 7.9 g (44 mmol) of N-bromosuccinimide were added thereto. The mixture was refluxed for 5 hours, and cooled to room temperature. The reaction mixture was washed with 50 ml of water twice and 50 ml portions of a brine solution, dried over anhydrous MgSO4, and distilled under a reduced pressure to obtain (E)-methyl-2-(2-bromomethyl-4-chlorophenyl)-3-methoxyacrylate containing a small amount of the starting material as an oil.


Step 4)


0.6 g (1.8 mmol) of the compound obtained in Step 3 was dissolved in 5 ml of acetonitrile, 0.5 g (3.6 mmol) of K2CO3 and 0.27 g (1.8 mmol) of 4-cyclopropylmethoxyphenol were added thereto, the resulting mixture was refluxed for 15 hours, and distilled under a reduced pressure to remove the solvent. To the residue, 30 ml of ethyl acetate was added, the resulting mixture was washed twice with water, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The concentrate was subjected to column chromatography to obtain 0.6 g (yield 85%) of (E)-methyl-2-(2-(4-(cyclopropylmethoxy)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate as a white solid.



1H NMR (300 MHz, CDCl3) d 7.58 (s, 1H), 7.55 (d, J=2.4 Hz, 1H), 7.26 (dd, J=8.1 Hz, 2.1 Hz, 1H), 7.07 (d, J=8.1 Hz, 1H), 6.83 (s, 4H), 4.85 (s, 2H), 3.82 (s, 3H), 3.73 (d, J=7.2 Hz, 2H), 3.69 (s, 3H), 1.27-1.22 (m, 1H), 0.65-0.59 (m, 2H), 0.35-0.30 (m, 2H)


EXAMPLE 2
Preparation of (E)-methyl-2-(2-((4-(2-methoxyethoxy)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate (Compound No. 274)

Step 1)


20.5 g (100 mmol) of 2-bromo-5-chlorotoluene was dissolved in 200 ml of carbon tetrachloride, 0.2 g (1 mmol) of AIBN and 19.6 g (110 mmol) of N-bromosuccinimide were added thereto, the resulting mixture was refluxed for 2 hours and cooled to room temperature. The reaction mixture was washed with 50 ml of water twice and with 50 ml of a brine solution, dried over anhydrous MgSO4, and distilled under a reduced pressure to obtain an oil containing a small amount of the starting material. The oil was dissolved in 20 ml of hexane, and recrystallized at room temperature to obtain 22.7 g (yield 80%) of 2-bromo-1-bromomethyl-5-chlorobenzene.



1H NMR (300 MHz, CDCl3) d 7.50 (d, 1H, J=8.7 Hz), 7.45 (d, 1H, J=2.4 Hz), 7.15 (dd, 1H, J=8.7 Hz, 2.4 Hz), 4.53 (s, 2H)


Step 2)


1.42 g (5 mmol) of the compound obtained in Step 1 was dissolved in 20 ml of acetonitrile, 1.38 g (10 mmol) of K2CO3 and 0.84 g (5 mmol) of 4-(2-methoxyethoxy)phenol were added thereto, which was refluxed for 15 hours. The reaction mixture was distilled under a reduced pressure to remove the solvent, and 20 ml of ethyl acetate was added thereto. The resulting mixture was washed twice with water, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The concentrate was subjected to column chromatography using 20% ethyl acetate/hexane to obtain 1.76 g (yield 95%) of 1-(2-bromo-5-chlorobenzyloxy)-4-(2-methoxyethoxy)benzene.


Step 3)


In a flask, 1.8 g (4.8 mmol) of the compound obtained in Step 2 was dissolved in 10 ml of anhydrous THF, and 1.34 ml (5.8 mmol) of triisopropyl borate was added thereto. The flask was cooled to −78° C. over a dry ice-acetone bath, 2.3 ml (5.8 mmol) of 2.5 M n-BuLi (in hexane) was added dropwise to the mixture over 15 min. After the reaction mixture was kept for 1 hour, the dry ice-acetone bath was removed, and 5 ml of 2 N HCl was added to the mixture. After stirring the mixture for 1 hour, the water layer was separated and extracted twice with 10 ml portions of ethyl acetate. The organic layers were combined, washed with a brine solution, dried over anhydrous MgSO4, and filtered under a reduced pressure to remove the solvent. The residue was recrystallized from ethyl acetate/hexane to obtain 1.12 g (yield 69%) of 2-((4-(2-methoxyethoxy)phenoxy)methyl)-4-chlorophenylboronic acid.


Step 4)


1.1 g (3.3 mmol) of the compound obtained in Step 3, 0.17 g (0.15 mmol) of tetrakis(triphenylphosphine) palladium and 1.96 g (9.0 mmol) of K3PO4 were placed in a flask, and 5 ml of dioxane and 1 ml of water were added thereto. After adding 0.73 g (3.0 mmol) of (E)-methyl-2-iodo-3-methoxy-2-propenoate thereto, the resulting mixture was stirred at 90° C. for 22 hours. The mixture was cooled to room temperature, and 10 ml of ethyl acetate was added thereto. The water layer was separated and extracted twice with 10 ml of ethyl acetate, and the organic layers were combined, washed with 20 ml of water and then with 20 ml of a brine solution, dried over anhydrous MgSO4 and concentrated under a reduced pressure. The resulting residue was subjected to column chromatography using 20% ethyl acetate/hexane as an eluent to obtain 0.87 g (yield 71%) of (E)-methyl-2-(2-((4-(2-methoxyethoxy)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate.



1H NMR (300 MHz, CDCl3) d 7.58 (s, 1H), 7.56 (d, J=2.3 Hz, 1H), 7.26 (dd, J=8.1 Hz, 2.1 Hz, 1H), 7.00 (d, J=8.0 Hz, 1H), 6.83 (s, 4H), 4.85 (s, 2H), 4.06 (t, J=6.1 Hz, 2H), 3.82 (s, 3H), 3.73 (t, J=6.1 Hz, 2H), 3.70 (s, 3H), 3.44 (s, 3H)


Similar procedures to Examples 1 and 2 were conducted to obtain the alpha-arylmethoxyacrylate derivatives as shown in Tables 1a to 11, and the 1H-NMR and MS analysis results of the representative compounds obtained are shown in Table 2a to 2c.











TABLE 2a





Com. No.

1H-NMR (CDCl3, 300 MHz) δ (ppm)

MS (m/e) (M+, int)

















15
7.58 (s, 1H), 7.51~6.88 (m, 8H), 5.15 (s, 2H), 3.97 (t,
439 (12), 348 (31),



J = 6.6 Hz, 2H), 3.80 (s, 3H), 3.68 (s, 3H), 2.22 (s,
145 (71), 43 (100)



3H), 1.82~1.73 (m, 2H), 1.58~1.26 (m, 6H), 0.91 (t, J =



6.9 Hz, 3H)


20
7.58 (s, 1H), 7.55~6.91 (m, 8H), 5.15 (s, 2H), 4.14 (t,
468 (21), 377 (57),



J = 5.7 Hz, 2H), 3.81 (s, 3H), 3.76~3.72 (m, 4H), 3.68
145 (100), 43 (61)



(s, 3H), 2.81 (t, J = 5.7 Hz, 2H), 2.60~2.57 (m, 4H),



2.22 (s, 3H)


133
7.58 (s, 1H), 7.56~6.85 (m, 8H), 5.33 (s, 1H), 4.96 (s,
372 (24), 205 (56),



2H) 3.81 (s, 3H), 3.69 (s, 3H), 3.31 (s, 6H)
145 (100), 102 (29)


161
7.56 (s, 1H), 7.51 (t, J = 4.1 Hz, 1H), 7.35 (d, J = 8.7
384 (21), 205 (39),



Hz, 2H), 7.34~7.29 (m, 2H), 7.16 (t, J = 4.1 Hz, 1H),
145 (100), 103 (25)



6.86 (d, J = 8.6 Hz, 2H), 5.43 (s, 1H), 4.95 (s, 2H),



4.23 (dd, J = 10.9 Hz, 5.0 Hz, 2H), 3.95 (td, J = 12.3



Hz, 2.2 Hz, 2H), 3.80 (s, 3H), 3.69 (s, 3H), 2.22~2.18



(m, 2H)


170
7.58 (s, 1H), 7.55~7.52 (m, 1H), 7.34~7.27 (m, 4H),
344 (31), 145 (100),



6.91~6.83 (m, 3H), 5.51 (qd, J = 47.7 Hz, 6.4 Hz, 1H),
130 (22), 102 (37)



3.80 (s, 3H), 3.69 (s, 3H), 1.59 (dd, J = 23.9 Hz, 6.4



Hz, 3H)


172
7.57 (s, 1H), 7.56~6.74 (m, 8H), 4.89 (s, 2H),
382 (22), 204 (29),



4.65~4.64 (m, 1H), 3.81 (s, 3H), 3.69 (s, 3H),
144 (100), 130 (14),



1.82~1.56 (m, 8H)
68 (22), 41 (41)


174
7.57 (s, 1H), 7.56~6.79 (m, 8H), 4.90 (s, 2H), 3.81 (s,
370 (13), 205 (22),



3H), 3.69 (s, 3H), 3.64 (d, J = 6.3 Hz, 2H), 2.04~2.01
144 (100), 131 (11),



(m, 1H), 0.99 (d, J = 6.6 Hz, 6H)
102 (11), 56 (29),




41 (27)


175
7.58 (s, 1H), 7.57~6.79 (m, 8H), 4.90 (s, 2H),
370 (24), 204 (34),



4.19~4.03 (m, 1H), 3.81 (s, 3H), 3.69 (s, 3H),
144 (100), 56 (26),



1.67~1.52 (m, 2H), 1.24 (d, J = 6.1 Hz, 3H), 0.96 (t, J =
41 (26)



7.4 Hz, 3H)


178
7.57 (s, 1H), 7.56~6.77 (m, 8H), 5.93~5.82 (m, 1H),
368 (18), 205 (57),



5.17~5.07 (m, 2H), 4.90 (s, 2H), 3.94 (t, J = 6.6 Hz,
145 (100), 131 (21),



2H), 3.81 (s, 3H), 3.69 (s, 3H), 2.40 (q, J = 6.6 Hz,
114 (17), 103 (14),



2H)
55 (25)


179
7.57 (s, 1H), 7.53 (d, J = 6.6 Hz, 1H), 7.34~7.30 (m,
354 (18), 204 (18),



2H), 7.16 (d, J = 6.3 Hz, 1H), 6.82 (s, 4H), 6.12~5.94
145 (100), 130 (24),



(m, 1H), 5.38 (dd, J = 17.4 Hz, 1.5 Hz, 1H), 5.25 (dd,
114 (14), 41 (19)



J = 10.5 Hz, 1.2 Hz, 1H), 4.09 (s, 2H), 4.45 (d, J =



5.4 Hz, 2H), 3.81 (s, 3H), 3.68 (s, 3H)


183
7.58 (s, 1H), 7.56~6.80 (m, 8H), 4.90 (s, 2H), 3.98 (q,
342 (25), 205 (21),



J = 6.9 Hz, 2H), 3.82 (s, 3H), 3.69 (s, 3H), 1.38 (t, J =
145 (100)



6.9 Hz, 3H)


192
7.87 (s, 1H), 7.57~6.77 (m, 8H), 4,90 (s, 2H), 3.85 (t,
356 (24), 205 (64),



J = 6.6 Hz, 2H), 3.80 (s, 3H), 3.69 (s, 3H), 1.83~1.71
145 (100), 102 (43)



(m, 2H), 1.01 (t, J = 7.5 Hz, 3H)


















TABLE 2b







197
7.58 (s, 1H), 7.57~7.09 (m, 5H), 6.51~6.46 (m, 3H),
424 (21), 205 (71),



5.92~5.71 (m, 1H), 5.05~4.95 (m, 2H), 4.93 (s, 2H),
144 (100), 130 (42),



3.91 (t, J = 6.5 Hz, 2H), 3.82 (s, 3H), 3.70 (s, 3H),
102 (51)



2.11~2.00 (m, 2H), 1.81~1.71 (m, 2H), 1.55~1.26 (m,



6H)


201
7.58 (s, 1H), 7.56~7.53 (m, 1H), 7.34~7.28 (m, 2H),
412 (13), 321 (48),



7.18~7.09 (m, 2H), 6.49~6.47 (m, 3H), 4.93 (s, 2H),
144 (100), 102 (44)



3.91 (t, J = 6.6 Hz, 2H), 3.82 (s, 3H), 3.69 (s, 3H),



1.77~1.70 (m, 2H), 1.43~1.26 (m, 8H), 0.89 (t, J = 6.3



Hz, 3H)


205
7.59 (s, 1H), 7.54~6.86 (m, 8H), 5.75~5.54 (m, 1H),
408 (24), 205 (100),



4.98 (s, 2H), 3.82 (s, 3H), 3.68 (s, 3H), 2.55~2.20 (m,
130 (47), 115 (30),



2H), 1.70 (t, J = 18.9 Hz, 3H)
102 (26)


206
7.60 (s, 1H), 7.59~6.85 (m, 8H), 5.97~5.79 (m, 1H),
386 (21), 205 (55),



4.97 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.15~2.68 (m,
145 (100), 130 (21)



2H), 2.18 (s, 3H)


211
7.61 (s, 1H), 7.60~6.49 (m, 8H), 6.18~5.91 (m, 1H),
354 (35), 204 (41),



5.31 (dd, J = 11.4 Hz, 7.0 Hz, 2H), 4.94 (s, 2H), 4.46
144 (100), 130 (30),



(t, J = 1.6 Hz, 2H), 3.82 (s, 3H), 3.69 (s, 3H)
102 (45), 41 (67)


215
7.58 (s, 1H), 7.57~6.48 (m, 8H), 4.92 (s, 2H), 3.83 (s,
368 (75), 205 (65),



3H), 3.80~3.70 (m, 2H), 3.68 (s, 3H), 0.54~0.41 (m,
144 (100), 129 (35),



5H)
102 (35)


217
7.59 (s, 1H), 7.58~6.48 (m, 8H), 5.58~5.41 (m, 1H),
382 (18), 214 (22),



4.93 (s, 2H), 4.42 (d, J = 6.8 Hz, 2H), 3.81 (s, 3H),
205 (79), 144 (100),



3.69 (s, 3H), 1.75 (d, J = 18.6 Hz, 6H)
102 (26), 69 (16)


219
7.59 (s, 1H), 7.58~6.47 (m, 8H), 4.93 (s, 2H), 3.82 (s,
370 (55), 205 (48),



3H), 3.69 (s, 3H), 3.67 (d, J = 6.6 Hz, 2H), 2.09~2.01
144 (100), 130 (22),



(m, 1H), 1.00 (d, J = 6.6 Hz, 6H)
102 (32)


220
7.58 (s, 1H), 7.57~6.47 (m, 8H), 4.93 (s, 2H), 3.91 (t,
398 (56), 204 (46),



J = 6.6 Hz, 2H), 3.82 (s, 3H), 3.69 (s, 3H), 1.79~1.25
144 (100), 130 (14),



(m, 8H), 0.90 (t, J = 6.6 Hz, 3H)
102 (17), 43 (21)


221
7.58 (s, 1H), 7.57~6.46 (m, 8H), 4.93 (s, 2H),
370 (46), 204 (39),



4.37~4.21 (m, 1H), 3.81 (s, 3H), 3.69 (s, 3H),
144 (100), 102 (28)



1.81~1.64 (m, 2H), 1.26 (d, J = 6.2 Hz, 3H), 0.95 (t, J =



7.4 Hz, 3H)


222
7.60 (s, 1H), 7.59~6.47 (m, 8H), 4.96 (s, 2H),
382 (47), 205 (69),



4.83~4.72 (m, 1H), 3.83 (s, 3H), 3.72 (s, 3H),
144 (100), 102 (24)



1.92~1.59 (m, 8H)


234
7.58 (s, 1H), 7.56~6.46 (m, 8H), 4.93 (s, 2H), 4.07 (t,
427 (14), 100 (100),



J = 5.7 Hz, 2H), 3.81 (s, 3H), 3.72 (t, J = 4.9 Hz, 4H),
55 (25), 41 (26)



3.69 (s, 3H), 2.77 (t, J = 5.7 Hz, 2H), 2.56 (t, J = 4.8



Hz, 4H)


















TABLE 2c







262
8.45 (s, 1H), 8.30~6.01 (m, 7H), 4.92 (s, 2H), 4.57 (s,
362 (21), 238 (41),



2H), 3.80 (s, 3H), 3.70 (s, 3H), 2.96 (br s, 1H)
178 (100), 136 (38),




101 (37)


264
7.58 (s, 1H), 7.56 (d, J = 2.4 Hz, 1H), 7.27 (dd, J =
388 (22), 239 (67),



8.1 Hz, 2.0 Hz, 1H), 7.08 (d, J = 8.1 Hz, 1H), 6.82 (s,
178 (75), 136 (43),



4H), 6.17~5.91 (m, 1H), 5.38 (dd, J = 17.1 Hz, 1.6 Hz,
41 (100)



1H), 5.26 (dd, J = 10.2 Hz, 1.6 Hz, 1H), 4.85 (s, 2H),



4.47 (td, J = 5.3 Hz, 1.6 Hz, 2H), 3.83 (s, 3H), 3.70



(s, 3H)


267
7.58 (s, 1H), 7.55 (d, J = 2.4 Hz, 1H), 7.26 (dd, J =
402 (42), 239 (60),



8.1 Hz, 2.1 Hz, 1H), 7.07 (d, J = 8.1 Hz, 1H), 6.83 (s,
178 (100), 136 (34),



4H), 4.85 (s, 2H), 3.82 (s, 3H), 3.73 (d, J = 7.2 Hz,
55 (43)



2H), 3.69 (s, 3H), 0.64~0.35 (m, 5H)


269
7.57 (s, 1H), 7.55 (d, J = 2.4 Hz, 1H), 7.26 (dd, J =
416 (41), 239 (54),



8.1 Hz, 2.0 Hz, 1H), 7.08 (d, J = 8.0 Hz, 1H), 6.83 (s,
178 (100),



4H), 5.51~5.34 (m, 1H), 4.84 (s, 2H), 4.52 (d, J = 5.3



Hz, 2H), 3.82 (s, 3H), 3.70 (s, 3H), 1.56 (d, J = 6.4



Hz, 6H)


271
7.58 (s, 1H), 7.56 (d, J = 2.3 Hz, 1H), 7.27 (dd, J =
432 (21), 239 (52),



8.0 Hz, 2.1 Hz, 1H), 7.09 (d, J = 8.0 Hz, 1H), 6.81 (s,
179 (100), 145 (42),



4H), 4.85 (s, 2H), 3.89 (t, J = 6.9 Hz, 2H), 3.82 (s,
43 (84)



3H), 3.69 (s, 3H), 1.78~1.26 (m, 8H), 0.89 (t, J = 4.1



Hz, 3H)


274
7.58 (s, 1H), 7.56 (d, J = 2.3 Hz, 1H), 7.26 (dd, J =
406 (16), 239 (69),



8.1 Hz, 2.1 Hz, 1H), 7.00 (d, J = 8.0 Hz, 1H), 6.83 (s,
179 (100), 136 (40),



4H), 4.85 (s, 2H), 4.06 (t, J = 6.1 Hz, 2H), 3.82 (s,
59 (43)



3H), 3.73 (t, J = 6.1 Hz, 2H), 3.70 (s, 3H), 3.44 (s,



3H)


319
7.58 (s, 1H), 7.34~6.46 (m, 7H), 4.86 (s, 2H),
388 (18), 223 (100),



4.37~4.21 (m, 1H), 3.82 (s, 3H), 3.70 (s, 3H),
163 (75), 59 (41)



1.81~1.64 (m, 2H), 1.26 (d, J = 6.2 Hz, 3H), 0.95 (t, J =



7.4 Hz, 3H)









EXAMPLE 3
Preparation of (E)-methyl-2-[2-((3-morpholinophenoxy)methyl)-4-chlorophenyl]-3-methoxyacrylate (Compound No. 386)

Step 1)


Method 1


526 mg (2.0 mmol) of 1-(benzyloxy)-3-bromobenzene, 209 μl (2.4 mmol) of morpholine, 283 mg (2.8 mmol) of sodium t-butoxide, 9 mg (0.005 mmol) of tris(dibenzylidine acetone)dipalladium(0) and 19 mg (0.015 mmol) of (±)-BINAP were placed in a flask, 5 ml of toluene was added thereto, and the mixture was stirred at 80° C. for 20 hours. The reaction mixture was cooled to room temperature, 20 ml of ethyl acetate was added thereto, and filtered through Cellite. The resulting filtrate was concentrated under a reduced pressure, and the residue was subjected to column chromatography using 30% ethyl acetate/hexane as an eluent to obtain 430 mg (yield 80%) of 4-(3-(benzyloxy)phenyl)morpholine.


Method 2


The procedure of Method 1 was repeated except for conducting the reaction at 120° C. for 10 min in an air-tighten microwave reactor to obtain 450 mg (yield 85%) of 4-(3-(benzyloxy)phenyl)morpholine.



1H NMR (300 MHz, CDCl3) δ 7.44-7.32 (m, 5H), 7.18 (t, 1H, J=8.7 Hz), 6.55-6.53 (m, 3H), 5.04 (s, 2H), 3.84 (t, 4H, J=4.7 Hz), 3.14 (t, 4H, J=4.9 Hz)


Step 2)


400 mg (1.4 mmol) of the compound obtained in Step 1 was dissolved in a mixture of 10 ml of methanol and 5 ml of ethyl acetate, and 32 mg of 10% palladium/carbon was added thereto. The mixture was placed in a hydrogenation reactor, kept under a hydrogen pressure of 30 to 40 psi for 36 hours, filtered through Cellite, and concentrated under a reduced pressure. The resulting residue was subjected to column chromatography using 5% methanol/methylene chloride as an eluent to obtain 240 mg (yield 80%) of 3-morpholinophenol as a solid form.


M.P.: 116-118° C.;



1H NMR (300 MHz, CDCl3) δ 7.13 (t, 1H, J=8.3 Hz), 6.50 (dd, 1H, J=8.3, 2.5 Hz), 6.40-6.32 (m, 2H), 4.73 (s, 1H), 3.85 (t, 4H, J=4.8 Hz), 3.15 (t, 4H, J=4.8 Hz);


MS (EI) M+ calc. 179.0946 for C10H13NO2. found 179.


Step 3)


58 mg (0.42 mmol) of (E)-methyl-2-(2-bromomethyl-4-chloro)phenyl-3-methoxyacrylate was dissolved in 2 ml of acetonitrile, 110 mg (0.84 mmol) of K2CO3 and 50 mg (0.28 mmol) of 3-morpholinophenol were added thereto, and the mixture was refluxed for 15 hours. The reaction mixture was distilled under a reduced pressure to remove the solvent, and 10 ml of ethyl acetate was added thereto. The resulting mixture was washed twice with water, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The concentrate was subjected to column chromatography using 20% ethyl acetate/hexane as an eluent to obtain 70 mg (yield 60%) of (E)-methyl-2-[2-((3-morpholinophenoxy)methyl)-4-chlorophenyl]-3-methoxyacrylate as an oil.



1H NMR (300 MHz, CDCl3) δ 7.58 (s, 1H), 7.56-7.09 (m, 4H), 6.54-6.39 (m, 3H), 4.95 (s, 2H), 3.87-3.83 (m, 4H), 3.81 (s, 3H), 3.68 (s, 3H), 3.07-3.02 (m, 4H);


MS (EI) M+ calc. 417.1343 for C22H24ClNO5. found 417


EXAMPLE 4
Preparation of (E)-methyl-2-[2-((3-(piperidin-1-yl)phenoxy)methyl)phenyl]-3-methoxyacrylate (Compound No. 388)

Step 1)


10.91 g (100 mmol) of 3-aminophenol was dissolved in 100 ml of toluene, and 18.5 g (220 mmol) of sodium bicarbonate and 16.0 ml (110 mmol) of 1,5-dibromopentane were added thereto, followed by refluxing the resulting mixture for 17 hours. The reaction mixture was cooled to room temperature, and 100 ml of water and 100 ml of ethyl acetate were added thereto. The water layer was separated, extracted twice with 100 ml portions of ethyl acetate, and the organic layers were combined, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The concentrate was subjected to column chromatography using 20% ethyl acetate/hexane as an eluent to obtain 12.9 g (yield 73%) of 3-(piperidin-1-yl)phenol as a solid.


M.P.: 112-114° C.;



1H NMR (300 MHz, CDCl3) δ 7.09 (t, 1H, J=7.9 Hz), 6.52 (dd, 1H, J=8.3, 2.3 Hz), 6.41 (t, 1H, J=2.3 Hz), 6.26 (dd, 1H, J=8.2, 2.4 Hz), 4.60 (s, 1H), 3.17-3.12 (m, 4H), 1.69-1.55 (m, 6H);


MS (EI) M+ calc. 177.1154 for C10H15NO. found 177.


Step 2)


96 mg (0.33 mmol) of (E)-methyl-2-(2-bromomethyl)phenyl-3-methoxyacrylate was dissolved in 2 ml of acetonitrile, and 58 mg (0.42 mmol) of K2CO3 and 50 mg (0.28 mmol) of the compound obtained in Step 1 were added thereto, followed by refluxing the mixture for 15 hours. The reaction mixture was distilled under a reduced pressure to remove the solvent, and 10 ml of ethyl acetate was added thereto. The resulting mixture was washed twice with water, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The residue was subjected to column chromatography using 20% ethyl acetate/hexane chloride as an eluent to obtain 56 mg (yield 52%) of (E)-methyl-2-[2-((3-(piperidin-1-yl)phenoxy)methyl)phenyl]-3-methoxyacrylate as a white solid.


M.P.: 64-66° C.;



1H NMR (300 MHz, CDCl3) δ 7.58 (s, 1H), 7.53-7.06 (m, 5H), 6.55-6.34 (m, 3H), 4.93 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.15-3.10 (m, 4H), 1.68-1.54 (m, 6H);


MS (EI) M+ calc. 381.194 for C23H27NO4. found 381 (10, M+), 205 (11), 145 (36), 43 (100).


EXAMPLE 5
Preparation of (E)-methyl-2-[2-((4-(N-isobutylamino)-2-fluorophenoxy)methyl)phenyl]-3-methoxyacrylate (Compound No. 425) and (E)-methyl-2-[2-((4-(N-isobutyl-N-methylamino)-2-fluorophenoxy)methyl)phenyl]-3-methoxyacrylate (Compound No. 426)

Step 1)


1.2 g (3.6 mmol) of (E)-methyl-2-(2-bromomethyl)phenyl-3-methoxyacrylate was dissolved in 20 ml of acetonitrile, 1.0 g (7.2 mmol) of K2CO3 and 0.57 g (3.6 mmol) of 2-fluoro-4-nitrophenol were added thereto, followed by refluxing the mixture for 15 hours. The reaction mixture was distilled under a reduced pressure to remove the solvent, 50 ml of ethyl acetate was added thereto. The resulting mixture was washed twice with water, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The residue was subjected to column chromatography using 30% ethyl acetate/hexane as a eluent to obtain 1.07 g (yield 82%) of (E)-methyl-2-[2-((2-fluoro-4-nitrophenoxy)methyl)phenyl]-3-methoxyacrylate as a white solid.


Step 2)


1.0 g (2.7 mmol) of the compound obtained in Step 1 was dissolved in a mixture of 5 ml of methanol and 5 ml of ethyl acetate, and 200 mg of 10% palladium/carbon was added thereto. The resulting mixture was placed in a hydrogenation reactor, and hydrogen gas was introduced therein with stirring the mixture for 18 hours. The reaction mixture was filtered through Cellite, and concentrated under a reduced pressure. The residue was subjected to column chromatography using 40% ethyl acetate/hexane as an eluent to obtain 0.84 g (yield 92%) of (E)-methyl-2-[2-((2-fluoro-4-aminophenoxy)methyl)phenyl]-3-methoxyacrylate.


Step 3)


150 mg (0.45 mmol) of the compound obtained in Step 2 was dissolved in 2 ml of methylene chloride, 134 mg (0.63 mmol) of NaBH(OAc)3 and 41 μl (0.45 mmol) of isobutyl aldehyde were added thereto. After stirring at room temperature for 6 hours, the reaction mixture was treated with a saturated sodium bicarbonate, and the water layer was separated, and extracted twice with 10 ml portions of methylene chloride. The organic layers were combined, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The residue was subjected to column chromatography using 20% ethyl acetate/hexane as an eluent to obtain 103 mg (yield 60%) of (E)-methyl-2-[2-((4-(N-isobutylamino)-2-fluorophenoxy)methyl)phenyl]-3-methoxyacrylate as an oil.



1H NMR (300 MHz, CDCl3) δ 7.59 (s, 1H), 7.58-7.56 (m, 1H), 7.34-7.29 (m, 2H), 7.16-7.13 (m, 1H), 6.76-6.70 (m, 1H), 6.38-6.21 (m, 2H), 4.90 (s, 2H), 3.79 (s, 3H), 3.68 (s, 3H), 3.54 (bs, 1H), 2.83 (d, J=6.6, 2H), 1.88-1.79 (m, 1H), 0.96-0.94 (m, 6H)


Step 4)


70 mg (0.18 mmol) of the compound obtained in Step 3 was dissolved in 1.5 ml of methylene chloride, and 57 mg (0.27 mmol) of NaBH(OAc)3 and 30 μl (0.40 mmol) of formaldehyde were added thereto. After stirring at room temperature for 22 hours, the reaction mixture was treated with a saturated sodium bicarbonate aqueous solution, and the water layer was separated and extracted twice with 10 ml of methylene chloride. The organic layers were combined, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The residue was subjected to column chromatography using 20% ethyl acetate/hexane as an eluent to obtain 50 mg (yield 73%) of (E)-methyl-2-[2-((4-(N-isobutyl-N-methylamino)-2-fluorophenoxy)methyl)phenyl]-3-methoxyacrylate as an oil.



1H NMR (300 MHz, CDCl3) δ 7.60 (s, 1H), 7.59-7.57 (m, 1H), 7.34-7.30 (m, 2H), 7.16-7.13 (m, 1H), 6.82-6.76 (m, 1H), 6.45-6.23 (m, 2H), 4.91 (s, 2H), 3.80 (s, 3H), 3.68 (s, 3H), 2.98 (d, J=7.2, 2H), 2.86 (s, 3H), 2.03-1.94 (m, 1H), 0.91-0.88 (m, 6H)


EXAMPLE 6
Preparation of (E)-methyl-2-[2-((3-(morpholinomethyl)phenoxy)-methyl)phenyl]-3-methoxyacrylate (Compound No. 404)

Step 1)


1.2 g (4.0 mmol) of (E)-methyl-2-(2-bromomethyl)phenyl-3-methoxyacrylate was dissolved in 20 ml of acetonitrile, 1.11 g (8.0 mmol) of K2CO3 and 0.59 g (4.8 mmol) of 3-hydroxybenzaldehyde were added thereto, followed by refluxing the mixture for 15 hours. The reaction mixture was distilled under a reduced pressure to remove the solvent, and 50 ml of ethyl acetate was added thereto. The resulting mixture was washed twice with water, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The residue was subjected to column chromatography using 30% ethyl acetate/hexane as an eluent to obtain 0.98 g (yield 75%) of (E)-methyl-2-[2-((3-formylphenoxy)methyl)phenyl]-3-methoxyacrylate as a white solid.


Step 2)


326 mg (1.0 mmol) of the compound obtained in Step 1 was dissolved in 5 ml of methylene chloride, and 297 mg (1.4 mmol) of NaBH(OAc)3 and 87 μl (1.0 mmol) of morpholine were added thereto. After stirring at room temperature for 4 hours, the reaction mixture was treated with a saturated sodium bicarbonate aqueous solution, and the water layer was separated, and extracted twice with 20 ml portions of methylene chloride. The organic layers were combined, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The residue was subjected to column chromatography using 3% methanol/chloroform as an eluent to obtain 385 mg (yield 97%) of (E)-methyl-2-[2-((3-(morpholinomethyl)phenoxy)methyl)phenyl]-3-methoxyacrylate as an oil.



1H NMR (300 MHz, CDCl3) δ 7.57 (s, 1H), 7.54-7.51 (m, 1H), 7.31-7.28 (m, 2H), 7.17-7.14 (m, 2H), 6.89-6.86 (m, 2H), 6.80-6.79 (m, 1H), 4.95 (s, 2H), 3.78 (s, 3H), 3.68 (s, 3H), 3.65-3.61 (m, 4H), 3.44 (m, 2H), 2.44-2.34 (m, 4H)


EXAMPLE 7
Preparation of (E)-methyl-2-[2-((6-(pyrrolidin-1-yl)pyridin-2-yloxy)-methyl)phenyl]-3-methoxyacrylate (Compound No. 415)

Step 1)


Method 1


In a dried microwave reactor, 526 mg (2.0 mmol) of 2-(benzyloxy)-6-bromopyridine and 1.70 ml (20 mmol) of pyrrolidine were placed, and the mixture was reacted using microwave at 150° C. for 10 min. The reaction mixture was mixed with 20 ml of water, extracted twice with 100 ml portions of ethyl acetate, and the organic layer was separated dried over anhydrous and kept under a reduced pressure to remove the solvent. The residue thus obtained was subjected to column chromatography using 10% ethyl acetate/hexane as an eluent to obtain 485 mg (yield 95%) of 2-(benzyloxy)-6-(pyrrolidin-1-yl)pyridine.


Method 2


In a dried microwave reactor into which an argon gas was introduced, 526 mg (2.0 mmol) of 2-(benzyloxy)-6-bromopyridine, 200 μl (2.4 mmol) of pyrrolidine, 283 mg (2.8 mmol) of sodium t-butoxide, 9 mg (0.005 mmol) of tris(dibenzylideneacetone)dipalladium(0) (0.5 mol % of Pd), 19 mg (0.015 mmol, 1.5 mol %) of (±)-BINAP and 3 ml of toluene were placed, followed by stirring and reacting the mixture using microwave at 120° C. for 10 min. The reaction mixture was diluted with 20 ml of ethyl acetate, filtered through Cellite, and kept under a reduced pressure to remove the solvent. The residue was subjected to column chromatography using 10% ethyl acetate/hexane as an eluent to obtain 470 mg (yield 92%) of 2-(benzyloxy)-6-(pyrrolidin-1-yl)pyridine.



1H NMR (300 MHz, CDCl3) δ 7.48-7.23 (m, 6H), 6.03-5.99 (m, 1H), 5.89-5.85 (m, 1H), 5.36 (s, 2H), 3.45-3.39 (m, 4H), 2.00-1.93 (m, 4H);


MS (EI) M+ calc. 164.095 for C9H12N2O. found 254 (23, M+), 163 (52), 91 (100), 70 (40), 65 (40).


Step 2)


450 mg (1.7 mmol) of the compound obtained in Step 1 was dissolved in a mixture of 5 ml of methanol and 5 ml of ethyl acetate, and 30 mg of 10% palladium/carbon was added thereto. The resulting mixture was placed in a hydrogenation reactor and hydrogen gas was introduced therein with stirring the mixture at room temperature for 18 hours. The reaction mixture was filtered through Cellite, and concentrated under a reduced pressure. The residue was subjected to column chromatography using 50% ethyl acetate/hexane as an eluent to obtain 260 mg (yield 92%) of 6-(pyrrolidin-1-yl)pyridin-2-ol.


M.P.: 154-158° C.;



1H NMR (300 MHz, CDCl3) δ 7.26 (td, 1H, J=8.7, 0.8 Hz), 5.75-5.70 (m, 1H), 5.25-5.21 (m, 1H), 4.65 (s, 1H), 3.45-3.39 (m, 4H), 2.00-1.93 (m, 4H);


MS (EI) M+ calc. 164.095 for C9H12N2O. found 164 (52, M+), 135 (45), 70 (85), 66 (28), 43 (100).


Step 3)


In a dried reactor, 63 mg (0.46 mmol) of K2CO3 and 50 mg (0.30 mmol) of the compound obtained in Step 2 were added to 4 ml of acetonitrile. The resulting mixture was stirred for 20 min, and 104 mg (0.36 mmol) of (E)-methyl-2-(2-bromomethyl)phenyl)-3-methoxy acrylate was added thereto. The resulting mixture was refluxed for 16 hours, cooled, distilled under a reduced pressure to remove the solvent, and then 10 ml of ethyl acetate was added thereto. The organic layer was separated, washed twice with water, dried over anhydrous MgSO4, and concentrated under a reduced pressure. The residue was subjected to column chromatography using 30% ethyl acetate/hexane as an eluent to obtain 70 mg (yield 64%) of (E)-methyl-2-[2-(6-(pyrrolidin-1-yl)pyridin-2-yloxy)methyl)phenyl]-3-methoxyacrylate.



1H NMR (300 MHz, CDCl3) δ 7.58 (s, 1H), 7.56-7.13 (m, 5H), 5.97-5.25 (m, 2H), 5.25 (s, 2H), 3.79 (s, 3H), 3.68 (s, 3H), 3.43-3.36 (m, 4H), 2.04-1.92 (m, 4H);


MS (EI) M+ calc. 368.1736 for C21H24N2O4. found 368 (31, M+), 205 (44), 163 (46), 145 (100), 103 (36), 40 (74).


Similar procedures to Examples 3 to 7 were conducted to obtain various alpha-arylmethoxyacrylate derivatives, and the 1H-NMR and MS analysis results of the representative compounds thus obtained were shown in Tables 3a to 3n.













TABLE 3a








MS (m/z,
mp


Com.


relative
(° C.)


No.
structure

1H NMR (CDCl3, TMS) δ (ppm)

intensity)



















347 


embedded image


7.57 (s, 1H), 7.54~7.51 (m, 1H), 7.36~7.33 (m, 1H), 7.31~7.26 (m, 1H), 7.18~7.15 (m, 1H), 6.85~6.81 (m, 4H), 4.91 (s, 2H), 3.81 (s, 3H), 3.77~3.73 (m, 2H), 3.69 (s, 3H), 3.61~3.58 (m, 2H), 3.05~2.99 (m, 4H), 2.13 (s, 3H)







348


embedded image


7.59 (s, 1H), 7.53~7.26 (m, 5H), 6.14~6.10 (m, 2H), 5.21 (s, 2H), 3.81~3.79 (m, 4H), 3.77 (s, 3H), 3.67 (s, 3H), 3.44~3.41 (m, 4H)
384 (34, M+), 205 (41), 179 (33), 145 (100), 130 (29), 103 (29)






349


embedded image


7.58 (s, 1H), 7.56~7.09 (m, 5H), 6.54~6.39 (m, 3H), 4.95 (s, 2H), 3.87~3.83 (m, 4H), 3.81 (s, 3H), 3.68 (s, 3H), 3.07~3.02 (m, 4H)







350


embedded image


7.58 (s, 1H), 7.56~7.53 (m, 1H), 7.35~7.27 (m, 2H), 7.19~7.12 (m, 3H), 6.85~6.81 (m, 2H), 4.95 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.57 (s, 2H), 1.78~1.69 (m, 1H), 0.88~0.85 (m, 2H), 0.39~0.29 (m, 2H)







351
embedded image

7.57 (s, 1H), 7.55~7.52 (m, 1H), 7.34~7.26 (m, 2H), 7.23~7.13 (m, 3H), 6.85~6.81 (m, 2H), 4.93 (s, 2H), 3.80 (s, 3H), 3.69 (s, 3H), 2.34 (t, J = 6.7, 2H), 1.55~1.19 (m, 8H), 0.84 (t, J = 6.3, 3H)







352


embedded image


7.57 (s, 1H), 7.54~7.17 (m, 6H), 6.83~6.78 (m, 1H), 6.61~6.56 (m, 1H), 4.87 (s, 2H), 3.80 (s, 3H), 3.69 (s, 3H), 3.19~3.11 (m, 4H), 1.50~1.26 (m, 8H), 0.95~0.88 (m, 6H)







353


embedded image


7.57 (s, 1H), 7.55~7.26 (m, 3H), 7.17~7.13 (m, 1H), 6.79~6.75 (m, 2H), 6.55~6.51 (m, 2H), 4.87 (s, 2H), 4.02 (b, 1H), 3.80 (s, 3H), 3.69 (s, 3H), 3.04 (t, J = 6.9, 2H), 1.62~1.39 (m, 4H), 0.94 (t, J = 7.1, 3H)


















TABLE 3b







354 
embedded image

7.57 (s, 1H), 7.56~7.54 (m, 1H), 7.34~7.17 (m, 5H), 6.82~6.77 (m, 1H), 6.68~6.62 (m, 1H), 5.56~5.48 (m, 4H), 4.87 (s, 2H), 3.81 (s, 3H), 3.74~3.70 (m, 4H), 3.69 (s, 3H), 1.69~1.66 (m, 6H)





355


embedded image


7.56 (s, 1H), 7.55~7.53 (m, 1H), 7.33~7.29 (m, 2H), 7.16~7.14 (m, 1H), 6.79~6.76 (m, 2H), 6.60~6.56 (m, 2H), 5.66~5.61 (m, 2H), 4.87 (s, 2H), 4.01 (b, 1H), 3.80 (s, 3H), 3.69 (s, 3H), 3.62 (d, J = 5.6, 2H), 1.70~1.68 (m, 3H)





356


embedded image


7.57 (s, 1H), 7.56~7.54 (m, 1H), 7.31~7.26 (m, 2H), 7.17~7.13 (m, 1H), 6.82~6.71 (m, 4H), 4.89 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.16~3.14 (m, 4H), 1.08~0.95 (m, 2H), 0.48~0.46 (m, 4H), 0.17~0.15 (m, 4H)





357


embedded image


7.56 (s, 1H), 7.55~7.53 (m, 1H), 7.31~7.25 (m, 2H), 7.15~7.11 (m, 1H), 6.78~6.75 (m, 2H), 6.56~6.53 (m, 2H), 4.87 (s, 2H), 4.01 (b, 1H), 3.81 (s, 3H), 3.68 (s, 3H), 2.89 (d, J = 6.9, 2H), 1.16~1.04 (m, 1H), 0.54~0.51 (m, 2H), 0.21~0.20 (m, 2H)





358


embedded image


7.57 (s, 1H), 7.55~7.53 (m, 1H), 7.34~7.26 (m, 2H), 7.17~7.13 (m, 1H), 6.81~6.75 (m, 2H), 6.55~6.51 (m, 2H), 4.87 (s, 2H), 4.03 (b, 1H), 3.81 (s, 3H), 3.69 (s, 3H), 3.05 (t, J = 7.3, 2H), 1.56~1.51 (m, 1H), 1.50~1.43 (m, 2H), 0.95~0.91 (m, 6H)





359


embedded image


7.57 (s, 1H), 7.55~7.52 (m, 1H), 7.36~7.25 (m, 2H), 7.17~7.13 (m, 1H), 6.86~6.73 (m, 3H), 6.59~6.54 (m, 1H), 4.89 (s, 2H), 4.01 (b, 1H), 3.82 (s, 3H), 3.69 (s, 3H), 3.63~3.14 (m, 2H), 2.83~2.67 (m, 1H), 2.58~2.54 (m, 2H)


















TABLE 3c







360 


embedded image


7.57 (s, 1H), 7.55~7.53 (m, 1H), 7.34~7.28 (m, 2H), 7.17~7.13 (m, 1H), 6.79~6.74 (m, 2H), 6.54~6.50 (m, 2H), 4.87 (s, 2H), 4.02 (b, 1H), 3.80 (s, 3H), 3.69 (s, 3H), 1.99~1.93 (m, 1H), 1.71~1.59 (m, 4H), 1.50~1.43 (m, 4H)





361


embedded image


7.57 (s, 1H), 7.55~7.53 (m, 1H), 7.34~7.26 (m, 2H), 7.17~7.12 (m, 1H), 6.79~6.73 (m, 2H), 6.56~6.50 (m, 2H), 4.87 (s, 2H), 4.01 (b, 1H), 3.80 (s, 3H), 3.69 (s, 3H), 2.86 (d, J = 6.7, 2H), 1.88~1.82 (m, 1H), 0.96 (d, J = 6.7, 6H)





362


embedded image


7.58 (s, 1H), 7.57~7.53 (m, 1H), 7.34~7.25 (m, 2H), 7.17~7.12 (m, 1H), 6.79~6.74 (m, 2H), 6.52~6.49 (m, 2H), 4.87 (s, 2H), 4.02 (b, 1H), 3.80 (s, 3H), 3.69 (s, 3H), 3.27~3.03 (m, 1H), 1.58~1.41 (m, 2H), 1.13 (d, J = 6.3, 3H), 0.93 (t, J = 7.5, 3H)





363
embedded image

7.57 (s, 1H), 7.55~7.53 (m, 1H), 7.34~7.26 (m, 2H), 7.17~7.13 (m, 1H), 6.80~6.75 (m, 2H), 6.59~6.54 (m, 2H), 4.88 (s, 2H), 4.02 (b, 1H), 3.80 (s, 3H), 3.68 (s, 3H), 3.58 (t, J = 4.9, 2H), 3.38 (s, 3H), 3.22 (t, J = 5.3, 2H)





364


embedded image


7.58 (s, 1H), 7.54~7.50 (m, 1H), 7.34~7. 13 (m, 3H), 6.64~6.56 (m, 3H), 4.87 (s, 2H), 4.01 (b, 1H), 3.82 (s, 3H), 3.69 (s, 3H), 2.89 (d, J = 6.7, 2H), l.31~1.25 (m, 1H), 0.97 (d, J = 6.5, 6H)





365


embedded image


7.58 (s, 1H), 7.55~7.50 (m, 1H), 7.34~7.23 (m, 2H), 7.18~7.13 (m, 1H), 6.65~6.57 (m, 3H), 4.86 (s, 2H), 4.01 (b, 1H), 3.82 (s, 3H), 3.69 (s, 3H), 3.34~3.28 (m, 1H), 1.59~1.48 (m, 2H), 1.14 (d, J = 6.3, 3H), 0.94 (t, J = 7.3, 3H)


















TABLE 3d







366 


embedded image


7.58 (s, 1H), 7.54~7.49 (m, 1H), 7.34~7.26 (m, 2H), 7.18~7.13 (m, 1H), 6.67~6.54 (m, 3H), 4.87 (s, 2H), 4.02 (b, 1H), 3.82 (s, 3H), 3.69 (s, 3H), 3.59 (t, J = 4.8, 2H), 3.38 (s, 3H), 3.25 (t, J = 5.3, 2H)





367


embedded image


7.59 (s, 1H), 7.54~7.49 (m, 1H), 7.35~7.26 (m, 2H), 7.18~7.14 (m, 1H), 6.89~6.79 (m, 1H), 6.64~6.54 (m, 2H), 5.87~5.70 (m, 2H), 5.19~5.08 (m, 4H), 4.87 (s, 2H), 3.82 (s, 3H), 3.70 (s, 3H), 3.67~3.64 (m, 4H)





368


embedded image


7.58 (s, 1H), 7.54~7.49 (m, 1H), 7.34~7.26 (m, 2H), 7.17~7.13 (m, 1H), 6.66~6.57 (m, 3H), 6.01~5.81 (m, 1H), 5.31~5.13 (m, 2H), 4.87 (s, 2H), 4.01 (b, 1H), 3.81 (s, 3H), 3.81~3.72 (m, 2H), 3.69 (s, 3H)





369


embedded image


7.58 (s, 1H), 7.54~7.50 (m, 1H), 7.34~7.29 (m, 2H), 7.18~7.13 (m, 1H), 6.66~6.58 (m, 3H), 4.87 (s, 2H), 4.01 (b, 2H), 3.82 (s, 3H), 3.70 (s, 3H), 3.07 (t, J = 6.9, 2H), 1.64~1.43 (m, 4H), 0.95 (t, J = 7.1, 3H)





370
embedded image

7.59 (s, 1H), 7.55~7.50 (m, 1H), 7.35~7.30 (m, 2H), 7.18~7.14 (m, 1H), 6.87~6.78 (m, 1H), 6.63~ 6.54 (m, 2H), 5.61~5.37 (m, 4H), 4.89 (s, 2H), 3.83 (s, 3H), 3.70 (s, 3H), 3.56~3.54 (m, 4H), 1.66~1.57 (m, 6H)





371


embedded image


7.60 (s, 1H), 7.58~7.50 (m, 1H), 7.34~7.26 (m, 2H), 7.19~7.13 (m, 1H), 6.66~6.58 (m, 3H), 5.69~5.58 (m, 2H), 4.87 (s, 2H), 4.01 (b, 1H), 3.82 (s, 3H), 3.69 (s, 3H), 3.66~3.63 (m, 2H), 1.71~1.68 (m, 3H)


















TABLE 3e







372


embedded image


7.59 (s, 1H), 7.58~7.49 (m, 1H), 7.34~7.26 (m, 2H), 7.17~7.13 (m, 1H), 6.66~6.56 (m, 3H), 4.86 (s, 2H), 4.01 (b, 1H), 3.82 (s, 3H), 3.69 (s, 3H), 2.91 (d, J = 6.9, 2H), 1.25~1.09 (m, 1H), 0.59~0.50 (m, 2H), 0.26~0.20 (m, 2H)





373


embedded image


7.58 (s, 1H), 7.52~7.50 (m, 1H), 7.34~7.26 (m, 2H), 7.17~7.12 (m, 1H), 6.64~6.58 (m, 3H), 4.86 (s, 2H), 4.03 (b, 1H), 3.82 (s, 3H), 3.69 (s, 3H), 3.08 (t, J = 7.3, 2H), 1.54~1.48 (m, 1H), 1.25~1.20 (m, 2H), 0.95~0.85 (m, 6H)





374 
embedded image

7.57 (s, 1H), 7.53~7.50 (m, 1H), 7.34~7.26 (m, 2H), 7.17~7.15 (m, 1H), 6.75~6.73 (m, 2H), 6.59~6.51 (m, 2H), 4.87 (s, 2H), 4.02 (b, 1H), 3.81 (s, 3H), 3.69 (s, 3H), 3.04 (t, J = 7.1, 2H), 1.59~1.54 (m, 2H), 1.32~1.25 (m, 6H), 0.89~0.81 (m, 3H)





375


embedded image


7.57 (s, 1H), 7.55~7.51 (m, 1H), 7.34~7.28 (m, 2H), 7.17~7.14 (m, 1H), 6.80~6.75 (m, 2H), 6.57~6.53 (m, 2H), 6.01~5.84 (m, 1H), 5.31~5.12 (m, 2H), 4.87 (s, 2H), 4.01 (b, 1H), 3.81 (s, 3H), 3.73~3.70 (m, 2H), 3.68 (s, 3H)





376


embedded image


7.70 (s, 1H), 7.58~7.54 (m, 1H), 7.38~7.26 (m, 2H), 7.17~7. 13 (m, 1H), 6.84~6.75 (m, 2H), 6.67~ 6.61 (m, 2H), 5.93~5.74 (m, 2H), 5.21~5.10 (m, 4H), 4.87 (s, 2H), 3.85~3.80 (m, 7H), 3.68 (s, 3H)





377


embedded image


7.57 (s, 1H), 7.55~7.52 (m, 1H), 7.34~7.30 (m, 2H), 7.17~7.03 (m, 1H), 6.28~6.20 (m, 4H), 4.91 (s, 2H), 4.08 (b, 1H), 3.8 (s, 3H), 3.59~3.56 (m, 2H), 3.70 (s, 3H), 3.37 (s, 3H), 3.26~3.23 (m, 2H)


















TABLE 3f







378 


embedded image


7.57 (s, 1H), 7.55~7.52 (m, 1H), 7.33~7.24 (m, 2H), 7.15~7.10 (m, 1H), 6.76~6.71 (m, 2H), 6.50~6.45 (m, 2H), 4.87 (s, 2H), 4.01 (b, 1H), 3.81 (s, 3H), 3.70 (s, 3H), 2.97~2.83 (m, 1H), 1.11~1.01 (m, 2H), 0.95 (d, J = 6.7, 3H), 0.85 (t, J = 7.3, 3H)





379


embedded image


7.58 (s, 1H), 7.55~7.50 (m, 1H), 7.34~7.30 (m, 2H), 7.17~7.14 (m, 1H), 7.04~6.98 (m, 2H), 6.27~6.17 (m, 2H), 4.91 (s, 2H), 4.01 (b, 1H), 3.81 (s, 3H), 3.70 (s, 3H), 3.37~3.31 (m, 1H), 1.28~1.13 (m, 2H), 0.98~0.90 (m, 6H)





380


embedded image


7.57 (s, 1H), 7.54~7.51 (m, 1H), 7.33~7.26 (m, 1H), 7.17~7.14 (m, 1H), 7.04~6.99 (m, 1H), 6.27~6.18 (m, 4H), 4.91 (s, 2H), 4.69 (b, 1H), 3.81 (s, 3H), 3.69 (s, 3H), 2.01~1.92 (m, 1H), 1.83~1.55 (m, 4H), 1.54~1.43 (m, 4H)





381


embedded image


7.57 (s, 1H), 7.55~7.52 (m, 1H), 7.31~7.25 (m, 2H), 7.19~7.15 (m, 1H), 7.12~6.99 (m, 2H), 6.35~6.29 (m, 2H), 5.91~5.75 (m, 1H), 5.18~5.10 (m, 2H), 4.91 (s, 2H), 4.01 (b, 1H), 3.88~3.77 (m, 2H), 3.80 (s, 3H), 3.68 (s, 3H)





382
embedded image

7.59 (s, 1H), 7.58~7.52 (m, 1H), 7.33~7.31 (m, 2H), 7.18~7.12 (m, 1H), 6.98~6.60 (m, 2H) 6.24~6.20 (m, 2H), 4.92 (s, 2H), 4.23 (b, 1H), 3.83 (s, 3H), 3.74~3.71 (m, 4H), 3.70 (s, 3H), 2.66 (t, J = 6.1, 2H), 2.59 (t, J = 5.7, 2H), 2.50~2.46 (m, 4H)





383


embedded image


7.57 (s, 1H), 7.56~7.54 (m, 1H), 7.34~7.29 (m, 1H), 7.17~7.15 (m, 1H), 7.02~6.99 (m, 1H), 6.25~6.18 (m, 4H), 4.91 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.07 (t, J = 7.3, 2H), 1.72~1.63 (m, 1H), 1.54~1.44 (m, 2H), 0.96~0.92 (m, 6H)




















TABLE 3g







384 


embedded image


7.57 (s, 1H), 7.56~7.53 (m, 1H), 7.33~7.29 (m, 1H), 7.17~7.15 (m, 1H), 7.05~6.99 (m, 1H), 6.27~6.17 (m, 4H), 4.91 (s, 2H), 4.01 (b, 1H), 3.81 (s, 3H), 3.70 (s, 3H), 2.91 (d, J = 6.9, 2H), 1.09~1.07 (m, 1H), 0.56~0.50 (m, 2H), 0.24~0.19 (m, 2H)







385
embedded image

7.57 (s, 1H), 7.56~7.54 (m, 1H), 7.34~7.30 (m, 1H), 7.17~7.15 (m, 1H), 7.02~6.99 (m, 1H), 6.25~6.17 (m, 4H), 4.91 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.59 (b, 1H), 3.06 (t, J = 7.2, 2H), 1.60~1.46 (m, 4H), 0.98~0.91 (m, 3H)







386


embedded image


7.59 (s, 1H), 7.57~7.07 (m, 4H), 6.55~6.37 (m, 3H), 4.89 (s, 2H), 3.87~3.84 (m, 4H), 3.83 (s, 3H), 3.70 (s, 3H), 3.16~3.11 (m, 4H)
417 (25, M+), 179 (100), 137 (22), 92 (24), 59 (27)






387


embedded image


7.58 (s, 1H), 7.30~7.07 (m, 4H), 6.58~6.31 (m, 3H), 4.88 (s, 2H), 3.83 (s, 3H), 3.69 (s, 3H), 3.17~3.11 (m, 4H), 1.69~1.55 (m, 6H)
415 (32, M+), 179 (100), 148 (38), 59 (44), 41 (46)
66-68





388


embedded image


7.58 (s, 1H), 7.53~7.06 (m, 5H), 6.55~6.34 (m, 3H), 4.93 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.15~3.10 (m, 4H), 1.68~1.54 (m, 6H)
381 (10, M+), 205 (11), 145 (36), 43 (100)
64-66





389


embedded image


7.56 (s, 1H), 7.35~6.97 (m, 10H), 6.97~6.55 (m, 3H), 4.90 (s, 2H), 3.77 (s, 3H), 3.66 (s, 3H), 3.28 (s, 3H)
403 (18, M+), 145 (100), 103 (40), 77 (30), 42 (18)






390


embedded image


7.56 (s, 1H), 7.54~6.95 (m, 9H), 6.59~6.42 (m, 3H), 4.85 (s, 2H), 3.78 (s, 3H), 3.67 (s, 3H), 3.29 (s, 3H)
437 (37, M+), 239 (32) 179 (100), 59 (22), 42 (23)






391


embedded image


7.58 (s, 1H), 7.55~7.08 (m, 5H), 6.54~6.37 (m, 3H), 4.93 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.22~3.17 (m, 4H), 2.59~2.54 (m, 4H), 2.34 (s, 3H)
397 (10, M+), 145 (28) 42 (100), 39 (63)






392


embedded image


7.58 (s, 1H), 7.54~7.04 (m, 5H), 6.25~6.15 (m, 3H), 4.94 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.27~3.21 (m, 4H), 2.17~2.01 (m, 4H)
367 (21, M+), 205 (23) 145 (100), 77 (26), 42 (14)
84-86




















TABLE 3h







393 


embedded image


7.76 (s, 1H), 7.59~7.55 (m, 2H), 7.36~7.14 (m, 4H), 6.93~6.75 (m, 1H), 5.20 (s, 2H), 3.88~3.83 (m, 4H), 3.79 (s, 3H), 3.67 (s, 3H), 3.07~3.02 (m, 4H)
384 (34, M+), 205 (38), 145 (100), 103 (17), 59 (11)
130-132





394


embedded image


7.75 (s, 1H), 7.58~7.56 (m, 2H), 7.30~7.24 (m, 2H), 7.10~7.06 (m, 1H), 6.74~6.70 (m, 1H), 5.16 (s, 2H), 3.88~3.83 (m, 4H), 3.81 (s, 3H), 3.68 (s, 3H), 3.07~3.03 (m, 4H)
418 (34, M+), 239 (50), 179 (100), 124 (26), 59 (17)






395


embedded image


7.58 (s, 1H), 7.32~7.13 (m, 5H), 6.57~6.46 (m, 2H), 5.04 (s, 2H), 3.85 (s, 3H), 3.65 (s, 3H), 2.71~2.66 (m, 4H), 1.62~1.48 (m, 6H)
382 (34, M+), 205 (18), 177 (31), 145 (100), 103 (18), 41 (20)






396
embedded image

7.57 (s, 1H), 7.35~7.14 (m, 7H), 6.97~6.96 (m, 1H), 6.82~6.57 (m, 4H), 5.11 (s, 2H), 3.80 (s, 3H), 3.61 (s, 3H), 3.07 (s, 3H)
403 (51, M+), 205 (20), 145 (100), 103 (39), 77 (26)






397


embedded image


7.59~7.56 (m, 1H), 7.54 (s, 1H), 7.49~7.13 (m, 4H), 6.96~6.90 (m, 1H), 6.68~6.63 (m, 1H), 5.18 (s, 2H), 3.79 (s, 3H), 3.67 (s, 3H), 3.25~3.19 (m, 4H), 2.04~1.97 (m, 4H)
368 (14, M+), 205 (37), 163 (31), 145 (100), 108 (38), 40 (89)
112-114





398


embedded image


7.57 (s, 1H), 7.52~7.14 (m, 4H), 7.86~7.84 (m, 4H), 4.91 (s, 2H), 3.87~3.82 (m, 4H), 3.81 (s, 3H), 3.69 (s, 3H), 3.06~3.01 (m, 4H)
383 (17, M+), 205 (33), 178 (100), 145 (57), 77 (64), 65 (69)
128-130





399


embedded image


7.57 (s, 1H), 7.53~7.15 (m, 4H), 6.85~6.83 (m, 4H), 4.90 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.03~2.98 (m, 4H), 1.73~1.53 (m, 6H)
381 (13, M+), 205 (21), 176 (100), 145 (30), 41 (31)






400


embedded image


7.60 (s, 1H), 7.58~6.74 (m, 13H), 4.96 (s, 2H), 3.83 (s, 3H), 3.70 (s, 3H), 3.24 (s, 3H)
403 (14, M+), 205 (28), 198 (100), 145 (56)






401


embedded image


7.59 (s, 1H), 7.54~7.13 (m, 4H), 6.86~6.80 (m, 2H), 6.51~6.47 (m, 2H), 4.89 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.25~3.18 (m, 4H), 2.00~1.94 (m, 4H)
367 (13, M+), 205 (16), 162 (100), 145 (41), 77 (20), 65 (13)
122-124




















TABLE 3i







402 


embedded image


7.58 (s, 1H), 7.55~7.52 (m, 1H), 7.33~7.30 (m, 2H), 7.17~7.15 (m, 2H), 6.89~6.87 (m, 2H), 6.79~6.76 (m, 1H), 4.95 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.46 (m, 2H), 2.44~2.34 (m, 8H), 2.28 (s, 3H)







403


embedded image


7.57 (s, 1H), 7.54~7.51 (m, 1H), 7.33~7.29 (m, 2H), 7.19~7. 14 (m, 3H), 6.85~6.82 (m, 2H), 4.94 (s, 2H), 3.79 (s, 3H), 3.68 (s, 3H), 3.42 (m, 2H), 2.43~2.32 (m, 8H), 2.27 (s, 3H)







404


embedded image


7.57 (s, 1H), 7.54~7.51 (m, 1H), 7.31~7.28 (m, 2H), 7.17~7.14 (m, 2H), 6.89~6.86 (m, 2H), 6.80~6.79 (m, 1H), 4.95 (s, 2H), 3.78 (s, 3H), 3.68 (s, 3H), 3.65~3.61 (m, 4H), 3.44 (m, 2H), 2.44~2.34 (m, 4H)







405


embedded image


7.58 (s, 1H), 7.55~7.52 (m, 1H), 7.33~7.28 (m, 2H), 7.19~7.15 (m, 3H), 6.86~6.82 (m, 2H), 4.96 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H), 3.67~3.64 (m, 4H), 3.41 (m, 2H), 2.42~2.39 (m, 4H)







406
embedded image

7.58 (s, 1H), 7.54~7.09 (m, 5H), 6.53~6.41 (m, 3H), 4.94 (s, 2H), 3.82 (s, 3H), 3.69 (s, 3H), 3.57~3.52 (m, 4H), 3.13~3.07 (m, 4H), 1.48 (s, 9H)
482 (11, M+), 205 (26), 145 (100), 57 (51)
138-140





407


embedded image


7.58 (s, 1H), 7.52~7.14 (m, 4H), 6.86~6.84 (m, 4H), 4.91 (s, 2H), 3.82 (s, 3H), 3.70 (s, 3H), 3.67~3.53 (m, 4H), 3.01~2.96 (m, 4H), 1.48 (s, 9H)
483 (12, M+) ,221 (62) 205 (45), 145 (100), 57 (52),






408


embedded image


7.59 (s, 1H), 7.58~7.54 (m, 1H), 7.34~7.30 (m, 2H), 7.17~7.14 (m, 1H), 6.93~6.66 (m, 3H), 4.96 (s, 2H), 3.85~3.82 (m, 4H), 3.81 (s, 3H), 3.63 (s, 3H), 3.06~3.03 (m, 4H)



















TABLE 3j







409 


embedded image


7.57 (s, 1H), 7.32~7.13 (m, 5H), 6.59~6.50 (m, 2H), 5.04 (s, 2H), 3.84 (s, 3H), 3.64 (s, 3H), 2.82~2.76 (m, 4H), 2.52~2.47 (m, 4H), 2.31 (s, 3H)
397 (34, M+), 205 (11), 145 (43), 70 (20), 43 (100)





410


embedded image


7.59 (s, 1H), 7.52~7.14 (m, 4H), 6.89~6.59 (m, 3H), 4.91 (s, 2H), 3.87~3.84 (m, 4H), 3.82 (s, 3H), 3.70 (s, 3H), 2.99~2.95 (m, 4H)
401 (11, M+), 205 (48), 145 (100), 103 (31), 77 (21)





411


embedded image


7.56 (s, 1H), 7.54~7.14 (m, 5H), 6.16~6.06 (m, 2H), 5.22 (s, 2H), 3.79 (s, 3H), 3.67 (s, 3H), 3.65~3.48 (m, 4H), 2.52~2.46 (m, 4H), 2.34 (s, 3H)
397 (10, M+), 205 (77), 145 (80), 70 (42), 42 (100)





412


embedded image


7.59 (s, 1H), 7.53~7.19 (m, 4H), 6.87~6.59 (m, 3H), 4.90 (s, 2H), 3.83 (s, 3H), 3.70 (s, 3H), 3.02~2.99 (m, 4H), 2.62~2.59 (m, 4H), 2.35 (s, 3H)
414 (11, M+), 247 (31), 205 (36), 145 (57), 43 (100)





413


embedded image


7.57 (s, 1H), 7.55~7.14 (m, 5H), 6.16~6.12 (m, 1H), 6.04~6.00 (m, 1H), 5.22 (s, 2H), 3.79 (s, 3H), 3.67 (s, 3H), 3.59~3.47 (m, 4H), 1.62~1.55 (m, 6H)
382 (27, M+), 205 (40), 177 (100), 145 (67), 103 (38), 41 (40)





414
embedded image

7.57 (s, 1H), 7.54~7.14 (m, 10H ), 6.08~6.03 (m, 2H), 5.25 (s, 2H), 3.79 (s, 3H), 3.68 (s, 3H), 3.42 (s, 3H)
404 (12, M+), 205 (40), 145 (100), 103 (38), 77 (28)





415


embedded image


7.58 (s, 1H), 7.56~7.13 (m, 5H), 5.97~5.25 (m, 2H), 5.25 (s, 2H), 3.79 (s, 3H), 3.68 (s, 3H), 3.43~3.36 (m, 4H), 2.04~1.92 (m, 4H)
368 (31, M+), 205 (44), 163 (46), 145 (100), 103 (36), 40 (74)





416


embedded image


7.59 (s, 1H), 7.53~7.14 (m, 4H), 6.90~6.81 (m, 1H), 6.65~6.57 (m, 2H), 4.89 (s, 2H), 3.82 (s, 3H), 3.70 (s, 3H), 2.93~2.87 (m, 4H), 1.75~1.53 (m, 6H)
399 (15, M+), 359 (32), 194 (63), 145 (85), 69 (39), 41 (100)





417


embedded image


7.62 (s, 1H), 7.56~7.11 (m, 8H), 6.78~6.62 (m, 4H), 4.96 (s, 2H), 3.85 (s, 3H), 3.71 (s, 3H), 3.21 (s, 3H)
421 (19, M+), 216 (56), 205 (39), 145 (100), 103 (34), 145 (100), 40 (38)



















TABLE 3k







418 


embedded image


7.59 (s, 1H), 7.55~7.14 (m, 4H), 6.68~6.59 (m, 3H), 4.88 (s, 2H), 3.82 (s, 3H), 3.70 (s, 3H), 3.26~3.20 (m, 4H), 1.95~1.88 (m, 4H)
385 (18, M+), 205 (32), 180 (72), 145 (100), 55 (28)





419
embedded image

7.59 (s, 1H), 7.48~7.17 (m, 4H), 6.84~6.82 (m, 1H), 6.67~6.59 (m, 2H), 4.91 (s, 2H), 3.83 (s, 3H), 3.70 (s, 3H), 3.59~3.54 (m, 4H), 2.94~2.88 (m, 4H), 1.47 (s, 9H)
500 (11, M+), 205 (62), 145 (77), 56 (49), 40 (100)





420


embedded image


7.59 (s, 1H), 7.57~7.55 (m, 1H), 7.37~7.26 (m, 2H), 7.17~7.12 (m, 1H), 6.79~6.70 (m, 1H), 6.43~6.35 (m, 1H), 6.26~6.19 (m, 1H), 5.99~5.82 (m, 1H), 5.31~5.13 (m, 2H), 4.91 (s, 2H), 3.80 (s, 3H), 3.78~3.70 (m, 2H), 3.68 (s, 3H), 3.61 (b, 1H)






421


embedded image


7.61 (s, 1H), 7.59~7.57 (m, 1H), 7.35~7.26 (m, 2H), 7.17~7.12 (m, 1H), 6.81~6.72 (m, 1H), 6.51~6.42 (m, 1H), 6.31~6.26 (m, 1H), 5.88~5.72 (m, 2H), 5.20~5.11 (m, 4H), 4.91 (s, 2H), 3.84~3.81 (m, 4H), 3.80 (s, 3H), 3.68 (s, 3H)






422


embedded image


7.61 (s, 1H), 7.60~7.58 (m, 1H), 7.34~7.30 (m, 2H), 7.16~7.13 (m, 1H), 6.82~6.76 (m, 1H), 6.61~6.39 (m, 2H), 4.92 (s, 2H), 3.80 (s, 3H), 3.68 (s, 3H), 3.17~3.15 (m, 4H), 1.01~0.95 (m, 2H), 0.52~0.46 (m, 4H), 0.20~0.15 (m, 4H)






423


embedded image


7.59 (s, 1H), 7.58~7.56 (m, 1H), 7.33~7.30 (m, 2H), 7.16~7.13 (m, 1H), 6.77~6.71 (m, 1H), 6.40~6.22 (m, 2H), 4.91 (s, 2H), 3.80 (s, 3H), 3.68 (s, 3H), 3.55 (b, 1H), 2.86 (d, J = 6.9, 2H), 1.08~1.03 (m, 1H), 0.56~0.50 (m, 2H), 0.23~0.18 (m, 2H)


















TABLE 3l







424 


embedded image


7.60 (s, 1H), 7.59~7.58 (m, 1H), 7.34~7.30 (m, 2H), 7.16~7.14 (m, 1H), 6.83~6.76 (m, 1H), 6.57~ 6.37 (m, 2H), 4.93 (s, 2H), 3.80 (s, 3H), 3.68 (s, 3H), 3.10 (d, J = 6.6, 2H), 2.88 (s, 3H), 0.96~0.94 (m, 1H), 0.52~0.46 (m, 2H), 0.19~0.14 (m, 2H)





425


embedded image


7.59 (s, 1H), 7.58~7.56 (m, 1H), 7.34~7.29 (m, 2H), 7.16~7.13 (m, 1H), 6.76~6.70 (m, 1H), 6.38~6.21 (m, 2H), 4.90 (s, 2H), 3.79 (s, 3H), 3.68 (s, 3H), 3.54 (b, 1H), 2.83 (d, J = 6.6, 2H), 1.88~1.79 (m, 1H), 0.96~0.94 (m, 6H)





426


embedded image


7.60 (s, 1H), 7.59~7.57 (m, 1H), 7.34~7.30 (m, 2H), 7.16~7.13 (m, 1H), 6.82~6.76 (m, 1H), 6.45~6.23 (m, 2H), 4.91 (s, 2H), 3.80 (s, 3H), 3.68 (s, 3H), 2.98 (d, J = 7.2, 2H), 2.86 (s, 3H), 2.03~1.94 (m, 1H), 0.91~0.88 (m, 6H)





427


embedded image


7.60 (s, 1H), 7.50~7.48 (m, 1H), 7.34~7.30 (m, 2H), 7.18~7.15 (m, 1H), 6.97~6.87 (m, 2H), 6.67~6.64 (m, 1H), 4.86 (s, 2H), 3.81 (s, 3H), 3.69 (s, 3H))





428


embedded image


7.57 (s, 1H), 7.53~7.49 (m, 1H), 7.33~7.30 (m, 2H), 7.17~7.14 (m, 1H), 7.02~6.93 (m, 2H), 6.63~6.60 (m, 1H), 4.88 (s, 2H), 4.14 (b, 1H), 3.80 (s, 3H), 3.69 (s, 3H), 2.94 (d, J = 6.9, 2H), 1.18~1.09 (m, 1H), 0.57~0.54 (m, 2H), 0.24~0.22 (m, 2H)





429
embedded image

7.59 (s, 1H), 7.54~7.49 (m, 1H), 7.33~7.32 (m, 3H), 7.28~7.26 (m, 2H), 7.14~7.13 (m, 1H), 4.96 (s, 2H), 3.82 (s, 3H), 3.70 (s, 3H), 2.81~2.78 (m, 4H), 0.92~0.84 (m, 2H), 0.36~0.33 (m, 4H), 0.03~0.01 (m, 4H)



















TABLE 3m







430 


embedded image


7.57 (s, 1H), 7.52~7.49 (m, 1H), 7.33~7.30 (m, 2H), 7.17~7.14 (m, 1H), 7.01~6.93 (m, 2H), 6.63~6.60 (m, 1H), 4.88 (s, 2H), 4.06 (b, 1H), 3.80 (s, 3H), 3.69 (s, 3H), 2.83 (d, J = 4.2, 2H), 1.94~1.83 (m, 1H), 0.98~0.96 (m, 6H)






431


embedded image


7.59 (s, 1H), 7.51~7.49 (m, 1H), 7.35~7.32 (m, 2H), 7.22~7.17 (m, 1H), 7.12~7.01 (m, 3H), 4.95 (s, 2H), 3.82 (s, 3H), 3.70 (s, 3H), 2.57 (d, J = 7.3, 2H), 2.53 (s, 3H), 1.80~1.72 (m, 1H), 0.90~0.88 (m, 6H)






432


embedded image


7.60 (s, 1H), 7.59~7.58 (m, 1H), 7.49~7.40 (m, 2H), 7.35~7.32 (m, 2H), 7.20~7.18 (m, 1H), 7.07~6.95 (m, 2H), 4.98 (s, 2H), 3.84 (s, 3H), 3.70 (s, 3H), 3.10 (d, J = 6.5, 2H), 2.88 (s, 3H), 1.02~1.04 (m, 1H), 0.41~0.37 (m, 2H), 0.19~0.14 (m, 2H)






433


embedded image


7.57 (s, 1H), 7.56~7.54 (m, 1H), 7.30~7.26 (m, 1H), 7.18~7.07 (m, 3H), 6.88~6.85 (m, 2H), 6.77~6.73 (m, 1H), 6.67~6.58 (m, 2H), 6.47~6.43 (m, 1H), 5.67 (s, 1H), 6.73 (m, 1H), 6.67~6.58 (m, 2H), 4.88 (s, 2H), 3.79 (s, 3H), 3.68 (s, 3H), 2.29 (s, 3H)
437 (53, M+), 239 (40), 179 (100), 59 (32)





434
embedded image

7.58 (s, 1H), 7.57~7.56 (m, 1H), 7.29~7.24 (m, 2H), 7.10~6.99 (m, 2H), 6.24~6.16 (m, 2H), 4.86 (s, 2H), 3.83 (s, 3H), 3.70 (s, 3H), 3.61 (s, 1H), 3.07 (t, J = 6.9, 2H), 1.59 (t, J = 6.5, 2H), 1.42~1.25 (m, 6H), 0.99 (t, J = 6.5, 3H)
431 (18, M+), 239 (30), 179 (100), 59 (34)





435


embedded image


7.58 (s, 1H), 7.57~7.50 (m, 1H), 7.45~7.26 (m, 4H), 7.25~7.14 (m, 1H), 6.92~6.84 (m, 2H), 4.97 (b, 1H), 4.88 (s, 2H), 3.79 (s, 3H), 3.68 (s, 3H), 2.29 (s, 3H)
382 (23, M+), 205 (30), 145 (100), 103 (32)




















TABLE 3n







436 


embedded image


8.18 (s, 1H), 7.79~7.75 (m, 1H), 7.56 (s, 1H), 7.51~7.44 (m, 1H), 7.40~7.26 (m, 3H), 7.24~7.16 (m, 4H), 6.93~6.89 (m, 2H), 5.06 (s, 2H), 3.70 (s, 3H), 3.61 (s, 3H)
414 (11, M+), 205 (43), 145 (100), 103 (42)






437


embedded image


7.87 ( s, 1H), 7.70 (s, 1H), 7.59 (s, 1H), 7.58~7.52 (m, 1H), 7.38~7.14 (m, 6H), 6.85~6.79 (m, 1H), 6.44~6.42 (m, 1H), 5.03 (s, 2H), 3.82 (s, 3H), 3.70 (s, 3H)
364 (29, M+), 332 (20), 205 (41), 145 (100), 103 (37)






438


embedded image


7.59 (s, 1H), 7.58~7.51 (m, 1H), 7.35~7.15 (m, 6H), 6.69~6.65 (m, 1H), 4.96 (s, 2H), 3.85~3.78 (m, 5H), 3.70 (s, 3H), 2.65~2.56 (m, 2H), 2.21~2.05 (m, 2H),
381 (12, M+), 205 (36), 145 (100), 59 (29)






439
embedded image

8.16 (d, J = 8.7 Hz, 2H), 7.72~7.12 (m, 1H), 7.61 (s, 1H), 7.56~7.53 (m, 2H), 7.37~7.30 (m, 4H), 7.26~7.21 (m, 1H), 7.01 (d, J = 8.7 Hz, 2H), 5.06 (s, 2H), 3.84 (s, 3H), 3.72 (s, 3H)
415 (7, M+), 205 (41), 182 (8), 145 (100)
138-139





440


embedded image


8.01 (d, J = 8.7 Hz, 2H), 7.67 (s, 1H), 7.54~7.16 (m, 5H), 7.12 (d, J = 8.7 Hz, 2H), 5.11 (s, 2H), 3.91 (s, 3H), 3.67 (s, 3H)
366 (24, M+), 204 (12), 145 (100), 102 (24), 77 (28)
146-147









TEST EXAMPLE 1
Inhibitory Effect on Osteoclast Formation

The inhibitory activities of the alpha-arylmethoxyacrylate derivatives prepared in the above Examples on the proliferation of osteoclasts were examined as follows.


(1-1) Isolation of Osteoclast Pregenitors and Induction of their Differentiation to Mature Osteoclasts


First, a bone marrow sample containing osteoclast pregenitor cells was isolated as follows. After sacrificing 7 to 9 week-old female mice by cervical dislocation, femur and tibia were excised aseptically while removing soft tissues attached thereto. After cutting both ends of the long bones, 1 ml of an enzyme solution containing 0.1% collagenase (Gibco), 0.05% trypsin and 0.5 mM EDTA (Gibco) was injected into the bone marrow cavity at one end using a syringe with a 26-gauge needle, and the bone marrow was then collected. After stirring the recovered bone marrow for 30 min, the precipitated bone marrow cells were collected, and cultured in α-minimum essential medium (α-MEM) supplemented with 10% FBS for 24 hrs. Then, non-adherent cells, which are osteoclast pregenitors, were aliquotted onto a culture plate at a density of 2×105 cells per well, and cultured for 8 days in α-MEM supplemented with 20 ng/ml of macrophage-colony stimulating factor (M-CSF, Peprotech, USA), 30 ng/ml RANKL (Peprotech, USA), and 0.3, 1.0 or 3 μM of the compounds of the Examples. Control cells were cultured at the same condition except not adding the compounds of the Examples.


(1-2) Evaluation of Inhibition of Osteoclast (TRAP-Positive Multinuclear Cell) Formation


After cell culture for 8 days, the adherent cells were washed with PBS and fixed with citrate-acetate-formaldehyde for 5 min. The fixed cells were incubated at 37° C. for 1 hr in acetate buffer (pH 5.0) containing naphthol AS-BI phosphate, fast Garnet GBC solution and 7 mM tartrate buffer (pH 5.0) to conduct TRAP (tartrate-resistant acid phosphatase) staining. After staining, TRAP-positive multinuclear cells having 3 or more nuclei were considered as osteoclast (see, Minkin, C., Calcif. Tissue Int. 34:285-290. 1982), and the inhibitory activities of the compounds of the Examples (0.3, 1.0 and 3.0 μM) on osteoclast formation compared to that of control are shown in Tables 4a and 4b.











TABLE 4a









Osteoclast formation inhibitory effects (%)












Compound No.
0.3 μM
1.0 μM
3.0 μM
















15
92
100
100



20
12
34
100



133
16
95
100



161
19
100
100



170
81
100
100



172
90
100
100



174
35
100
100



175
14
100
100



178
89
100
100



179
84
100
100



183
96
100
100



192
68
100
100



197
100
100
100



201
12
70
100



205
90
100
100



206
35
100
100



211
23
96
100



215
51
100
100



217
94
100
100



219
99
100
100



220
100
100
100



221
39
100
100



222
69
100
100



229
100
100
100



234
25
100
100



262
93
100
100



264
98
100
100



267
98
100
100



269
97
100
100



271
94
100
100



274
100
100
100



319
82
100
100




















TABLE 4b









Osteoclast formation inhibitory effects (%)












Compound No.
0.1 μM
0.3 μM
1 μM
3 μM














348

24
99
100


349
2
53
100
100


361


95
100


367


87
100


372


91
100


380

8
51
100


381

5
67
100


383

6
94
100


386
100
100
100
100


387
1
100
100
100


388
0
99
100
100


390
2
94
99
100


391

4
50
100


392

2
95
100


394
0
98
100
100


397

22
100
100


399
0
70
100
100


400
0
53
99
100


404

9
63
100


406
41
100
100
100


408
0
52
100
100


413

11
100
100


414

32
100
100


415
5
57
100
100


416
0
75
100
100


417

24
97
100


418

11
81
100


421

14
100
100


422
11
98
100
100


423
0
51
100
100


424
0
92
100
100


425
0
51
100
100


426
25
100
100
100









As can be seen from Tables 4a and 4b, the α-arylmethoxyacrylate compounds of the present invention have an excellent inhibitory effect on osteoclast formation.


(1-3) Evaluation of Inhibitory Effect on Resorption Activity of Osteoclast


In order to evaluate the effect of the compounds synthesized in the Examples on the resorption activity of the osteoclasts, the differentiated osteoclasts were cultured on a calcium phosphate-coated plate (OAAS™, OCT, Korea) (Choi et al., Eur. J. Immunol. 31:2179-2188, 2001). After finishing culture, the plate was washed with distilled water, and 50 μl/well of 5% sodium hypochlorite was added to the plate. The plate was let alone for 5 min, washed again with distilled water to remove the adherent cells, and dried at room temperature. Then, the area of formed resorption pits was calculated by means of Image Pro Plus software (Media Cybernetics Ver. 3.0). Reduction (%) of the resorption pit area of osteoclasts treated with the compounds of the Examples as compared to that of the control are shown in Table 5.














TABLE 5







Compound No.
0.3 uM
1 uM
3 uM





















15
41
57
93



20

26
47



133
75
99
100



161
94
100
100



170
0
100
100



172
90
100
100



174
100
100
100



175
99
100
100



178
97
100
100



179
86
100
100



183
100
100
100



192
100
100
100



197
100
100
100



201
100
100
100



205

100
100



206
97
100
100



211
96
100
100



215
97
100
100



217

100
100



219

88
100



220
42
70
73



221
94
100
100



222
100.0
100
100



229
0.00
100
100



234
95
100
100



262
93
100
100



264
100
100
100



267
100
100
100



269
100
100
100



271
30
94
42



274
100
100
100



319
0
100
100










As can be seen from Table 5, the resorption pit area in the plate treated with one of the compounds of the Examples was remarkably reduced as compared to that of the control, and the resorption activity of osteoclast was almost completely inhibited when more than 0.1 μM of any of the compounds of the Examples was used. This result demonstrates that the α-arylmethoxyacrylate compounds of the present invention have an excellent inhibitory activity against osteoclast.


TEST EXAMPLE 2
Cytotoxicity Test

(2-1) Cytotoxicity Against Osteoclast Pregenitor


In order examine the toxicities of the compounds of the Examples against osteoclast pregenitors, pregenitor cells were aliquotted onto a 96-well microplate at a density of 2×105 cells per well, treated with 2, 4 and 8 μM of the test compounds, respectively, and cultured for 48 hrs in α-MEM supplemented with 20 ng/ml of M-CSF (Peprotech, USA) using 37° C. incubator (5% CO2). At 3 hrs before the culture was finished, 50 μl of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solution (50 μg/ml) was added to each well. Upon completion of the cell culture, the supernatant was removed, and the precipitated dye was reacted with 100 μl of isopropanol/0.04 N HCl at room temperature for 30 min to dissolve it. The absorbances of the wells were measured at 550 nm, and the relative absorbance of each well relative to that of the control (set as 100) is shown in Table 6.














TABLE 6







Compound No.
2 uM
4 uM
8 uM





















Control
100
100
100



133
93
104
102



205
101
109
104



206
102
102
108



211
88
103
103



215
96
102
107



217
90
96
107



219
90
100
99



220
89
98
96



221
96
96
99



222
97
108
101










As can be seen form Table 6, the α-arylmethoxyacrylate compounds of the present invention have little cytotoxicity against undifferentiated bone marrow cells.


(2-2) Cytotoxicity Test Against Osteoblast


In order to examine the toxicity of the compounds of the Examples against osteoblasts, human osteosarcoma-derived cell line, MG-63 (ATCC No. CRL-1427) cells were treated with 0.1, 0.3, 1.0 and 3.0 μM of the compounds of the Examples, and cultured in DMEM supplemented with 10% FBS (fetal bovine serum). Cytotoxicity was measured in accordance with the method of (2-1), and the results are shown in Tables 7a and 7b.













TABLE 7a





Compound No.
0.11 uM
0.33 uM
1 uM
3 uM



















Control
100
100
100
100


15

105
85
76


20

106
106
105


133
103
108
107
103


179
117
111
108
106


197
112
101
111
103


211
103
101
105
106


234

98
115
97


264
103
107
104
92


269

81
94
88


271

114
79
64


274
113
108
106
94





















TABLE 7b







Compound No.
0.33 μM
1 μM
3 μM





















Control
100
100
100



386
101
69
68



388
105
84
81



391
104
99
111



394
113
105
110



399
96
76
65



404
83
92
94










As can be seen from Tables 7a and 7b, the compounds of the Examples have little cytotoxicity against osteoblasts.


TEST EXAMPLE 3
Clinical Test

(3-1) Bone Mineral Density (BMD) Determination of Female Mice Undergone Ovariectomy (Control)


The effect of Compound Nos. 274 and 388 of the present invention on BMD of female mice with osteoporosis induced by ovariectomy was examined as follows.


Specifically, after anesthetizing female mice used as a control by abdominal administration with a mixture of 10 mg/kg body weight of Ketamin HCl (Ketara) and 0.15 ml/kg body weight of 2% Xylazine HCl (Roupun), the lumbar dorsum of each mouse was shaved bilaterally and the exposed skin was prepared for aseptic surgery by a 10% povidone-iodine scrub followed by a 70% alcohol wipe. A 1-cm incision was made in the central region of the abdomen, and the ovaries were identified with caution not to damage the main organs such as the liver and diaphragm. The ovaries were ligated with a suture thread, and then severed. Thereafter, each organ was relocated to its original position, and the incision was closed with a suture thread in an interrupted pattern. After ovariectomy, the mice were injected with 0.088 mg/kg body weight of gentamicin to prevent infection.


To investigate the change in BMD of the mice, bone mineral density was measured before the ovariectomy and every two weeks for 8 weeks after the ovariectomy using a bone mineral densitometer, XCT 540 Research SA (Stratec, Germany). Specifically, BMD measurement was made at a voxel size of 0.1 mm×0.1 mm, threshold values of 280 mg/cm2 for cancellous bone and 500 mg/cm2 for compact bone, and the analysis sites at the proximal tibias were determined by Scout scans (10 mm/sec). BMD was measured at three slices at the determined sites by CT scans (7 mm/sec), and the measurement was performed twice or more at the same site.


(3-2) Determination of BMD in Female Multiparous Mice after Ovariectomy


Multiparous mice each weighing 250 to 350 g were subjected to ovariectomy in accordance with the method of (3-1). From the 2nd day to 12th week after the ovariectomy, the mice were injected subcutaneously once a day with 0.5 and 1 mg/kg body weight/day of Compound Nos. 274 and 388, respectively. Alternatively, the mice were administered orally with 2.5 and 7.5 mg/kg body weight/day of Compound Nos. 274 and 388. BMD was measured before the ovariectomy, and during the period of the 2nd week to 11th week after the ovariectomy.



FIGS. 1
a and 1b show the result obtained with the Compound No. 274, wherein the controls not treated with the Compound No. 274 showed a decrease of BMD (subcutaneous injection: 4.0%, oral administration: 6.3%), while the mice treated with the Compound No. 274 showed no decrease of BMD in the case of subcutaneous injection, and little increase of BMD (0.8%) in the case of oral administration.


On the other hand, FIGS. 2a and 2b show the result obtained with the Compound No. 388, wherein the controls not treated with the Compound No. 388 showed sudden decreases of BMD (subcutaneous injection: 15.4%; oral administration: 15.6%) after 8 weeks, while the mice treated with the Compound No. 388 showed sudden decreases in the BMD level (subcutaneous injection: 5.0% at 0.5 mg/kg and 6.7% at 1 mg/kg; oral administration: 10.6% at 2.5 mg/kg and 10.2% at 7.5 mg/kg).


Therefore, it can be concluded that the α-arylmethoxyacrylate compounds of the present invention are effective for preventing and treating osteoporosis.


TEST EXAMPLE 4
Pharmacokinetics

(4-1) In Vitro Pharmacokinetics


Metabolic stabilities of the compounds of the Examples were examined by employing microsome samples prepared from the human liver.


Each 20 μM of the compounds were reacted with 1 mg/ml of liver microsome and the half-lives and one-hour stabilities of the compounds were determined. The results are shown in Table 8.











TABLE 8





Comp. No.
Half-life (min)
One-hour stability (%)

















15
36.9
33


161
45.6
40


179
>180
70


211
106
60


234
>180
83


264
>180
80


267
>180
70


274
>180
72


386
40.66


388
30.71


391
107.2 ± 18.6


394
 55.5 ± 12.0


399
43.0 ± 1.8


404
57.4 ± 2.3


415
24.87


416
43.86


426
42.7 ± 2.1









The above result demonstrates that the alpha-arylmethoxyacrylate derivatives of the present invention have a high metabolic stability.


(4-2) In Vivo Pharmacokinetics Using Female Mice


(4-2-1) Administration of Compounds and Serum Separation


Female mice each weighing about 250 g were divided into groups of 5 mice each. The mice were anesthetized with ether, catheterized in their femoral artery and vein, respectively, and administered with 0.5 mg/kg body weight of compounds 234 and 274, and 5 mg/kg body weight of compounds 388, 404 and 415, respectively, via intravenous injection. Alternatively, 15 mg/kg body weight of compound 274 and 10 mg/kg body weight of compound 234 were orally administered to the mice.


At 0, 5, 10, 15 and 30 minutes and 1, 2, 4, 6, 9 and 12 hours after the intravenous injection, or at 0, 10, 20 and 40 minutes and 1, 2, 4, 6, 9 and 12 hours after the oral administration, 0.3 ml blood samples were taken from the mice through femoral artery. The blood samples were kept on an ice bath for 30 minutes and centrifuged at 3,000 rpm for 10 minutes to obtain a supernatant (serum). The supernatant samples were stored at −20° C.


(4-2-2) Determination of the Concentration of the Compounds in the Serum


In the following experiment, HPLC-grade methanol and acetonitrile (Merck) and a HPLC system (Shimadzu LC-10AD) were used.


Standard solution: Compounds 234, 274, 388, 404 and 415 were respectively dissolved in methanol to a concentration of 1 mg/ml to obtain stock solutions. The stock solutions were diluted with methanol to obtain standard solutions having concentrations of 40, 20, 10, 2, 1, 0.5, 0.2, 0.05 and 0.02 μg/ml, respectively.


Standard calibration curve: A stand calibration curve was prepared by employing calibration concentrations of 0.002, 0.005, 0.02, 0.05, 0.1, 0.2, 1, 2 and 4 μg/ml.


10 μl each of the standard solutions prepared above was added to 100 μl of normal serum sample and diluted 10 times. 250 μl of acetonitrile was added to the resulting dilution and the mixture was centrifuged for 10 minutes to obtain a supernatant. 300 μl of the supernatant was dried by evaporation under a nitrogen atmosphere and reconstituted by adding 50 μl of methanol. 20 μl of the resulting solution was analyzed by HPLC to prepare a standard calibration curve. HPLC was performed with Shimadzu ODS2 column (4.6×250 mm, 5 μm by employing a mixture of methanol/water (90/10(v/v)) as the mobile phase at a flow rate of 1.2 ml/min, and measuring the absorbance at 240 nm.


The resulting stand calibration curves exhibited good linearity.


Extraction: 100 μl of the serum sample obtained in (4-2-1) was put into a 1 ml microtube and 10 μl of methanol was added thereto. 250 μl of acetonitrile was added to the microtube and the mixture was centrifuged for 10 minutes to obtain a supernatant. 300 μl of the supernatant was dried by evaporation under a nitrogen atmosphere and reconstituted by adding 50 μl of methanol. 20 μl of the resulting solution was analyzed by the HPLC method as above.


(4-2-3) Determination of Pharmacokinetic Parameters


Average concentrations of the compounds in the serum samples were plotted in a semi-log scale against the time lapsed after the administration, and the pharmacokinetic parameters were determined as a non-compartment open model by employing WinNonlin® program (Pharsight Corporation). Average values for the pharmacokinetic parameters are shown in Table 9.















TABLE 9





Comp.
Admin.
AUC*1
CL*2
Half-life
Vd*3
Bioavailability


No.
route
(μg × hr/ml)
(ml/hr)
(hr)
(ml/kg)
(%)





















234
I.V.
477.6 ± 96.4 
1.1 ± 0.2
10.6 ± 4.8
15.2 ± 5.2
35.4



Oral
680.4 ± 426.2
19.5 ± 10.6
12.8 ± 7.7
 354.4 ± 245.6


274
I.V.
47.2 ± 25.8
14.3 ± 9.7 
 3.5 ± 2.3
50.3 ± 9.8
8.5



Oral
80.6 ± 45.4
7.5 ± 3.4
 4.2 ± 3.7
 43.4 ± 46.8


388
I.V.
112.6 ± 13.0 
2.9 ± 0.5
 7.3 ± 1.2
27.4 ± 2.0



404
I.V.
97.2 ± 14.8
3.4 ± 0.5
10.2 ± 1.9
46.2 ± 6.4



415
I.V.
176.4 ± 32.9 
1.9 ± 0.4
10.79 ± 1.61
27.0 ± 1.2






*1AUC: Area under the curve of blood concentration versus time


*2CL: Clearance


*3Vd: Volume of distribution






As shown in Table 9, half-lives of the inventive compounds upon I.V. administration ranged from 3.5 to 11 hours. This result shows that the inventive compounds have suitable in vivo stabilities for use as drugs.


Test Example 5
In Vivo Toxicity Test

In order to determine the acute toxicities of the compounds prepared in Examples, 6-week old specific pathogen-free (SPF) rats each weighing about 20 g were divided into groups of 10 rat each.


In case of subcutaneous administration, each of the compounds was dissolved in 5% PEG400 solution to a concentration of 20 mg/ml, the resulting solution was serially diluted with 5% PEG400 solution to concentrations of 5, 2.5, 1.25 and 0.625 mg/ml, and the dilutions were subcutaneously injected once to the rats at a dose of 10 ml/kg body weight.


In case of oral administration, each of the compounds was dissolved in soybean oil to a concentration of 180 mg/ml, the resulting solution was serially diluted with soybean oil to concentrations of 80, 20 and 5 mg/ml, and the dilutions were orally administered once to the rats at a dose of 20 ml/kg body weight.


Citrate-phosphate buffer (pH 4.0) was used as a solvent for the preparation of the injection and oral formulations.


During 2 weeks after the administration of the compounds, the death rate, clinical symptoms and weight changes of the rats were observed, and hematological and biochemical tests on blood samples were performed. Then, the rats were sacrificed and the internal organs were visually examined to check any abnormal signs in the organs of chest and abdomen.


LD50 values of the compounds depending on the administration route are shown in Table 10.












TABLE 10









LD50 (mg/kg body weight)










Comp. No.
SC
PO












15
>50
>2000


169
10
>1000


179
42
500


211
51
500


234
7
100-300


264
25
269


267
5
100-300


274
11
460


388



110-130









As shown in Table 10, most of the compounds exhibited only low levels of toxicity.


While some of the preferred embodiments of the subject invention have been described and illustrated, various changes and modifications can be made therein without departing from the spirit of the present invention defined in the appended claims.

Claims
  • 1. A methoxyacrylate derivative which is selected from the group consisting of: (E)-methyl 2-(2-((4-octylphenoxy)methyl)phenyl-3-methoxyacrylate;(E)-methyl 2-(2-((4-(cyclopropylmethoxy)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(2-methoxyethoxy)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(allyloxy)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(2-methoxyethoxy)phenoxy)methyl)-4-fluorophenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(allyloxy)phenoxy)methyl)-4-fluorophenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(1-methylpropaneoxy)phenoxy)methyl)-4-fluorophenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((3-(2-morpholinoethoxy)phenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((3-(1,3-dioxan-2-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(allyloxy)phenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((3-morpholinophenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((3-(piperidin-1-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(piperidin-1-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((3-(4-methylpiperizan-1-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(N-isobutyl-N-methylamino)-2-fluorophenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(N-cyclopropylmethylamino)-2-fluorophenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((4-(N-cyclopropylmethyl-N-methylamino)-2-fluorophenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-3-fluoro-4-(piperidin-1-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((3-(morpholinomethyl)phenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((3-(N-methyl-N-phenylamino)phenoxy)methyl)-4-chlorophenyl)-3-methoxyacrylate;(E)-methyl 2-(-((6-(pyrrolidin-1-yl)pyridin-2-yloxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((6-(piperidin-1-yl)pyridin-2-yloxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-5-(morpholino)pyridin-2-yloxy)methyl)phenyl)-3-methoxyacrylate; and(E)-methyl 2-(2-((6-(morpholino)pyridin-2-yloxy)methyl)phenyl)-3-methoxyacrylate.
  • 2. The methoxyacrylate derivative of claim 1 which is selected from the group consisting of: (E)-methyl 2-(2-((3-(2-morpholinoethoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-(2-((3-(1,3-dioxan-2-yl)phenoxy)methyl)phenyl)-3-methoxyacrylate;(E)-methyl 2-)2-))3-)morpholinomethyl)phenoxy)methyl)phenyl)-3-methoxyacrylate.
Priority Claims (1)
Number Date Country Kind
10-2004-0046644 Jun 2004 KR national
Parent Case Info

This is a divisional application of U.S. Ser. No. 11/570,160 filed on Dec. 7, 2006, which is a national stage application under 35 U.S.C. 371 of PCT/KR2005/01935 filed on Jun. 22, 2005, which claims priority from Korean patent application 10-2004-0046644 filed on Jun. 22, 2004, all of which are incorporated herein by reference.

Foreign Referenced Citations (6)
Number Date Country
0414153 Feb 1991 EP
0463488 Jan 1992 EP
0335519 Oct 1998 EP
WO 9218487 Oct 1992 WO
9520569 Aug 1995 WO
03087032 Oct 2003 WO
Related Publications (1)
Number Date Country
20100256367 A1 Oct 2010 US
Divisions (1)
Number Date Country
Parent 11570160 US
Child 12578660 US