Patent Application
20130287697
1. Field of the Invention
The present invention relates to a pharmaceutical microparticle for embolization, and particularly relates to a pharmaceutical microparticle for embolization suitable for use as an effective drug carrier, and has biodegradable and X-ray imaging properties.
2. Description of Related Art
Cancer has been the first leading cause on top 10 causes of death in Taiwan, wherein hepatoma is the first leading cause of death for men and the second leading cause of death for women. Therapeutic treatment of hepatoma includes transcatheter arterial embolization (TAE), percutaneous ethanol injection (PEI), cryotherapy, radiotherapy, and chemotherapy, and so on.
In the case of using transcatheter arterial embolization in hepatoma therapy, nutrition fed to the liver tumor tissue is almost entirely provided through the liver artery, therefore when the liver artery is blocked, normal liver tissue will be able to continue to survive and not become subject to necrosis because the normal liver tissue would still have portal veins to supply blood flow; in contrast, the liver cancer tissue would become necrotic due to nutrition deficiency.
Currently, the embolization compound for TAE includes a degradable material, such as gelatin; and non-degradable material, such as polyvinyl alcohol (PVA), vinyl based resin, drug eluting beads (DEB), and so on. Among these, gelatin is cheaper but cannot work to effectively carry chemotherapy drugs, thereby resulting in poor treatment effect. On the other hand, some non-degradable materials can carry chemotherapy drugs effectively but are expensive and cannot degrade in vivo, thereby resulting in new vascular formation to supply cancer cells and resulting to poor treatment effect. In addition, the above-mentioned embolization compounds do not possess X-ray imaging properties for tracking the position thereof.
Therefore, what is needed in the art is to develop a pharmaceutical microparticle for embolization having drug carrying ability as well as biodegradable and X-ray imaging properties, so as to improve success rate of TAE and reduce undesired side-effects for prolongation of patient's life.
An object of the present invention is to provide a pharmaceutical microparticle for embolization having drug carrying ability as well as biodegradable and X-ray imaging properties, so as to improve success rate of TAE.
To achieve the above and other objects, the present invention provides a pharmaceutical microparticle for embolization, which includes: a thermoresponsive polymer, a first enhancer, a contrast agent, and a solvent, wherein the particle size of pharmaceutical microparticle for embolization is 100-750 μm, and preferably 150-350 μm.
The pharmaceutical microparticle for embolization according to the present invention may come in any shape, such as spherical shape, spherical-like shape, pyramidal shape, columnar shape, cubical shape, irregular shape, etc., and preferably spherical shape.
In addition, the pharmaceutical microparticle for embolization according to the present invention may further include a chemical drug. The chemical drug is not particularly limited and may be any pharmaceutical agent that can provide a therapeutic benefit to a subject, and preferably a radioactive element compound, a fat-soluble drug, or a water-soluble drug. Examples of drug for cancer therapy include doxorubicin, bevacizumab, sorafenib, irinotecan, thalidomide, resveratrol, curcumin, antibiotics, and so on.
Furthermore, the radioactive element compound may preferably be rhenium-188 radioactive element compound, yttrium-90 radioactive element compound, or holmium-166 radioactive element compound, but is not limited thereto, and any well-known compound having a therapeutic benefit to a subject may be used, for example, rhenium-118 radioactive element compound, strontium-89 radioactive element compound, iodine-125 element compound, and so on.
In the present invention, the radioactive element compound is not particularly limited, and may be rhenium-188-N,N′-1,2-ethanediylbis-L-cysteine diethylester (ECD), yttrium-90, or holmium-166, rhenium-188-1-hydroxy-1,1-ethylidene disodium phosphonate (HEDP), rhenium-188 radioactive liposomes, or iodine-125-5-iodo-2′-deoxyuridine (IUdR) etc., and preferably rhenium-188-N,N′-1,2-ethanediylbis-L-cysteine diethylester (ECD), yttrium-90, and holmium-166.
In the pharmaceutical microparticle for embolization according to the present invention, the type of the contrast agent is not particularly limited, and may be any known component serving as a contrast agent for as long as it can function as a contrast agent, and is preferably lipodol or BaSO4.
In the pharmaceutical microparticle for embolization according to the present invention, the thermoresponsive polymer is present in an amount of 0.3-4.0 parts by weight, and the first enhancer is present in an amount present in an amount of 0.6-9.0 parts by weight; preferably, the thermoresponsive polymer is present in an amount present in an amount of 0.3-3.5 parts by weight, and the first enhancer is present in an amount present in an amount of 0.6-7.0 parts by weight; and more preferably, the more responsive polymer is present in an amount present in an amount of 0.3-0.4 parts by weight, and the first enhancer is present in an amount present in an amount of 0.6-0.7 parts by weight. Herein, the thermoresponsive polymer may be selected from the group consisting of polyethylene glycol (PEG), cetyl alcohol, glycerol monostearate, ethylene glycerol monostearate, poloxamer 188 (Pluronic F68), and myristyl alcohol; preferably, the thermoresponsive polymer may be a combination of glycerol monostearate, ethylene glycerol monostearate, and poloxamer 188 (Pluronic F68). In addition, the first enhancer is selected from the group consisting of stearic acid, polyethylene glycol (PEG), stearylamine, poly(lactic-co-glycolic acid) (PLGA), polyethylene oxide, α-cyclodextrin, and polycaprolactone. Preferably, the first enhancer is a combination including stearic acid and polycaprolactone.
In addition, the pharmaceutical microparticle for embolization according to the present invention may further comprise one selected from the group consisting of a thickener and a second enhancer. Preferably, the pharmaceutical microparticle for embolization may further comprise a thickener and a second enhancer. The thickener is present in an amount present in an amount of 0.00-0.05 parts by weight, preferably 0.05-0.1 parts by weight, and more preferably 0.05-0.06 parts by weight.
Herein, the thickener is at least one selected from the group consisting of lecithin, cholesterol, and dextrin; and preferably, cholesterol.
The second enhancer may be selected from the group consisting of stearic acid, polyethylene glycol (PEG), stearylamine, poly(lactic-co-glycolic acid) (PLGA), polyethylene oxide, α-cyclodextrin, and polycaprolactone.
The present invention also provides a pharmaceutical microparticle for embolization, comprising: a thermoresponsive polymer; an enhancer; a contrast agent; a solvent; a thickener, wherein the more responsive polymer is selected from the group consisting of glycerol monostearate, ethylene glycerol monostearate, polycaprolactone and polyether polyol; the enhancer is selected from the group consisting of polycaprolactone, stearic acid, and cetyl alcohol; the contrast agent and the solvent are lipodol; and the thickener is selected from the group consisting of cholesterol, lecithin, and dextrin.
In the pharmaceutical microparticle for embolization according to the present invention, the thermoresponsive polymer is present in an amount present in an amount of 0.3-3.5 parts by weight, the enhancer is present in an amount present in an amount of 0.6-7.0 parts by weight, the thickener is present in an amount present in an amount of 0.00-0.05 parts by weight. Preferably, the more responsive polymer is present in an amount of 0.3-0.4 parts by weight, the enhancer is present in an amount of 0.6-0.7 parts by weight, the thickener is present in an amount of 0.00-0.05 parts by weight.
Accordingly, the components included in the pharmaceutical microparticle for embolization according to the present invention are common components used for current clinical pharmaceuticals. Therefore, comparing with other newly developed pharmaceuticals, the pharmaceutical microparticle for embolization according to the present invention may shorten the clinical trial period and accelerate application in clinical medicine.
The pharmaceutical microparticle for embolization according to the present invention may be prepared by any known process, and preferably spray granulation. Since spray granulation has the advantages of instant drying, high product quality, multi-level drying, and simple process etc., such a process has been widely used in pharmaceutical, chemical, material, food, and cosmetic industry.
The pharmaceutical microparticle for embolization according to the present invention has effective drug carrying ability, and biodegradable and X-ray imaging properties. The X-ray imaging property may be used to observe the stationary position of the pharmaceutical microparticle for embolization, and after injection of the pharmaceutical microparticle for embolization into the subject, an image can be taken directly by a X-ray imager, thereby confirming the arrival of the drug at the target site. In addition, the biodegradable property may inhibit the long-term accumulation of embolism in the extracellular matrix, and the blood vessel will be embolized by the microparticles after the injection of the pharmaceutical microparticle for embolization into the subject causing death of the cancer cell due to nutrient deficiency. After a period of time, the microparticles will degrade, and the remaining cancer cells still use the same blood vessel. Therefore, it may avoid the cancer cell from angiogenesis or transferring to other sites in the body by using another blood vessel. Furthermore, the pharmaceutical microparticle for embolization according to the present invention may carry a chemical drug to the target sited and slowly release the drug to improve the treatment and ease the patient's conditions efficiently.
Thus, the pharmaceutical microparticle for embolization according to the present invention may improve success rate of TAE, reduce undesired side effects for prolongation of patient's life, and can be used to treat liver cancer, kidney cancer, uterine fibroids, spleen embolism, and so on in clinic.
Hereinafter, exemplary embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments disclosed below, but can be implemented in various forms. The following embodiments are described in order to enable those of ordinary skill in the art to embody and practice the present invention, and those skilled in the art will appreciate that various modifications, additions and substitutions are possible without departing from the scope and spirit of the invention as disclosed in the accompanying claims.
First, the components of microparticles were thoroughly mixed uniformly in amounts as listed in Table 1.
Then, the syringe 11 and atomizing nozzle 3 of the injection pump 1 were heated to a temperature of 60-75° C., and maintained in such a temperature range.
After that, the mixed microparticle raw materials were injected into the granulation apparatus in a feeding rate of 10 ml/min and melted into liquid form by a hot water bath, and then in monodispersed particle generation, the liquid raw materials were directly injected into the pressure atomizing nozzle 3 under application of external excitation to form the microparticles having a uniform particle diameter. The atomizing nozzle used in the monodispersed particle generation was shown in
Further, in the binary-fluid type process, the spray was conducted through the atomizing nozzle 3 at a gas input rate of 30 L/min to obtain the embolization-oriented pharmaceutical microparticles. The atomizing nozzle of the binary-fluid spray granulation is shown in
In this example, the mixture may pass through the UV germicidal lamp 4 and the gas filter to produce sterile microparticles.
Finally, the product of the pharmaceutical microparticles for embolization was collected by the collecting and packaging device 7, dried by the exhausters 8, and then pictured by a microscope. The particle size of the microparticle in the picture was measured according to the scale bar. The photomicrograph of the microparticle produced by the monodispersed particle generation is shown in
As shown in the results of the synthetic examples 1-3, the preparation method can produce the sterile microparticles having a uniform particle size without aggregation. In addition, such microparticles have a low degradation rate, and the effect of slow drug release can be realized when encapsulating a chemical drug. Furthermore, this preparation method has a high yield and without pollution from organic solvents. In addition, our microspheres have drug delivery ability, as well as biodegradable and X-ray imaging properties, which are useful in clinical practice.
The making and using of the embodiments of the disclosure are discussed in detail below. It should be appreciated, however, that the embodiments provide many applicable inventive concepts that can be embodied in a wide variety of specific contexts. The specific embodiments discussed are merely illustrative, and do not limit the scope of the disclosure.
| Number | Date | Country | Kind |
|---|---|---|---|
| 101115152 | Apr 2012 | TW | national |
