Phenylalkyl derivatives, with pharmaceutical activity

The present invention relates to phenylalkyl derivatives of general formula ##STR2## and the compound 5,7-dimethyl-2-ethyl-3-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-3,5-dichlorobenzyl]imidazo-[4,5-b]pyridine, the isomer mixtures thereof, the tautomers, enantiomers and salts thereof, more particularly for pharmaceutical use the physiologically acceptable salts thereof with inorganic or organic acids or bases.
In the above general formula
n represents the number 0 or 1,
A represents a straight-chained or branched alkylene group,
B represents an oxygen atom, a carbonyl, hydroxy-methylene, sulphenyl, sulphinyl or sulphonyl group, a straight-chained or branched alkylene group, a C.sub.2-4 -alkylidene group, a 1,1-cycloalkylene group or an imino group optionally substituted by an alkyl group or by a C.sub.1-4 -alkanoyl group,
R.sub.a represents a chlorine or bromine atom, a hydroxy, alkylsulphonyloxy, phenylsulphonyloxy or phenylalkyl-sulphonyloxy group or a group of the formula ##STR3## wherein one of the groups D.sub.1, D.sub.2 or D.sub.3 represents a methylene or imino group and the remaining groups D.sub.1 to D.sub.3 are each methine groups, whilst additionally a methine group may be replaced by a nitrogen atom and one of the methine groups may be substituted by the group R.sub.5 and, optionally, another of the methine groups may be substituted by the group R4,
none, one or two of the groups D.sub.4, D.sub.5, D.sub.6 or D.sub.7 may represent a nitrogen atom and the remaining groups D.sub.4, D.sub.5, D.sub.6 or D.sub.7 may each represent methine groups, whilst additionally a methine group may be substituted by the group R.sub.4 and another methine group may be substituted by the group R.sub.5,
E represents a carbon-carbon bond, an oxygen or sulphur atom, a hydroxymethylene or carbonyl group or an imino group optionally substituted by a C.sub.1-6 -alkyl group, by a cycloalkyl group, by a C.sub.2-5 -alkanoyl group or by an allyl, phenyl or benzyl group,
X represents an oxygen or sulphur,atom or an imino group optionally substituted by an alkyl, phenyl or phenylalkyl group,
R.sub.1 represents a straight-chained or branched C.sub.1-9 -alkyl group, a straight-chained or branched C.sub.2-6 -alkenyl or C.sub.2-6 -alkynyl group, whilst the above-mentioned saturated and unsaturated alkyl moieties may each be substituted by a cycloalkyl group, by a fluorine, chlorine or bromine atom, by a hydroxy, amino, alkylamino, dialkylamino or .alpha.,.alpha.-difluoroethane group, or R.sub.1 may represent a C.sub.1-4 -perfluoroalkyl group or a cycloalkyl group, which may be mono- or di-substituted by a trifluoromethyl group or by an alkyl group,
R.sub.2 represents a hydrogen, fluorine, chlorine or bromine atom, a C.sub.1-5 -alkyl or C.sub.1-5 -perfluoroalkyl group, a cyano or nitro group,
R.sub.3 represents a hydrogen atom, a cyano group, a C.sub.1-6 -alkyl group optionally substituted by a hydroxy or alkoxy group, a C.sub.1-6 -perfluoroalkyl group, a C.sub.3-6 -alkenyl group optionally substituted by fluorine atoms, a phenylalkyl or phenyl(C.sub.2-4)alkenyl group, a C.sub.1-5 -alkyl group which is terminally substituted by an imidazol-1-yl, triazolyl, tetrazolyl, phthalimido, R.sub.6 COO--, R.sub.7 S--, R.sub.7 SO--, R.sub.7 SO.sub.2 --, R.sub.7 CO--, R.sub.7 NHCOO--, R.sub.7 NHCO--, R.sub.7 NHCONR.sub.7 --, R.sub.8 CONR.sub.7 - or R.sub.8 SO.sub.2 NR.sub.7 - group, whilst
the triazolyl group may additionally be mono- or di-substituted by an acetoxy or alkyl group and
R.sub.6 represents a C.sub.1-8 -alkyl or C.sub.1-8 -perfluoroalkyl group, a cycloalkyl, phenyl, benzyl, phenylethyl, adamantyl, naphthyl, naphthylmethyl or naphthylethyl group,
R.sub.7 represents a hydrogen atom or has the meanings given for R.sub.6 hereinbefore,
R.sub.8 has the meanings given for R.sub.7 hereinbefore or represents a piperazino group optionally substituted by an alkyl or phenyl group in the 4-position, or R.sub.8 represents a pyrrolidino, piperidino, hexamethyleneimino, morpholino, R.sub.7 O- or (R.sub.7).sub.2 N-group,
R.sub.4 represents a hydrogen, fluorine, chlorine or bromine atom, a trifluoromethyl group, a cycloalkyl group, a C.sub.1-6 -alkyl group optionally substituted by a cycloalkyl, hydroxy, alkoxy, alkylamino, dialkylamino, alkoxycarbonyl, alkylaminocarbonyl or dialkylamino-carbonyl group, and
R.sub.5 represents a hydrogen, fluorine, chlorine or bromine atom, a straight-chained or branched C.sub.1-6 -alkyl or C.sub.1-6 -perfluoroalkyl group, a C.sub.2-6 -alkenyl or C.sub.2-6 -alkynyl group, whilst the above-mentioned alkyl and alkenyl moieties may each be mono- or disubstituted by a heteroaryl, hydroxy, alkoxy, amino, alkylamino, dialkylamino, alkylcarbonylamino, N-alkyl-alkylcarbonylamino, carboxy, alkoxycarbonyl, alkylcarbonyloxy, piperidinocarbonyl, morpholinocarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, tetrazol-5-yl, tetrazol-5-yl-aminocarbonyl, alkylsulphenyl, alkylsulphinyl, alkylsulphonyl, aminosulphonyl, alkylaminosulphonyl, dialkylaminosulphonyl, alkylsulphonylaminocarbonyl, heteroarylaminosulphonyl or alkylcarbonylaminosulphonyl group,
a C.sub.1-7 -alkoxy group which is substituted in the 2, 3-, 4-, 5-, 6- or 7-position by an imidazolyl, tetrazolyl, benzimidazolyl or tetrahydrobenzimidazolyl group, or a phenylalkoxy group optionally substituted in the alkoxy moiety by a 1H-tetrazol-5-yl or 1-triphenylmethyl-tetrazol-5-yl group,
a carboxy group or a group which is metabolically converted into a carboxy group in vivo,
a C.sub.1-4 -alkylsulphonyloxy group, a benzenesulphonyloxy or phenylalkylsulphonyloxy group,
an acylamino group optionally substituted at the nitrogen atom by a C.sub.1-6 -alkyl group or by a phenyl, cycloalkyl, phenylalkyl, cycloalkylalkyl, bicyclohexyl or biphenyl group, wherein the acyl group is a C.sub.1-7 -alkanoyl group, an alkoxycarbonyl group with a total of 2 to 4 carbon atoms, a C.sub.1-6 -alkylsulphonyl group, a benzoyl, benzenesulphonyl, phenylalkanesulphonyl, naphthylenesulphonyl, cycloalkylcarbonyl, phenylalkanoyl or cycloalkylalkanoyl group, whilst the above-mentioned phenyl nuclei may each be mono- or di-substituted by a fluorine, chlorine or bromine atom or by a methyl or methoxy group and the substituents may be identical or different,
a phthalimino, homophthalimino, 2-carboxyphenylcarbonyl-amino or 2-carboxyphenylmethylamino group, whilst a carbonyl group in a phthalimino group may be replaced by a methylene, alkyl-methylene or dialkyl-methylene group and a methylene group in a homophthalimino group may be substituted by one or two alkyl groups, wherein the phenyl nuclei in any of said groups may be additionally mono- or di-substituted by alkyl or alkoxy groups and may be additionally wholly or partially hydrogenated, in which the substituents may be identical or different,
a 5-, 6- or 7-membered alkyleneimino or alkenyleneimino group optionally substituted by one or two alkyl groups or by a tetramethylene or pentamethylene group, wherein a methylene group of the alkyleneimino or alkenyleneimino group may be replaced by a carbonyl or sulphonyl group,
a bicycloalkane-2,3-dicarboxylic acid imino or bicycloalkene-2,3-dicarboxylic acid imino group, wherein the bicycloalkane and bicycloalkene moieties each contain 9 or 10 carbon atoms, may be substituted by 1, 2 or 3 methyl groups and may have an endomethylene group replaced by an oxygen atom,
a glutaric acid imino group, in which the n-propylene group may be perfluorinated, and may be substituted by one or two alkyl groups or by a tetramethylene or pentamethylene group,
a maleic acid imido or succinimido group optionally mono- or di-substituted by an alkyl or phenyl group, wherein the substituents may be identical or different,
a 5-membered heteroaromatic ring bound via a carbon atom or via an imino group, which contains an imino group, an oxygen or sulphur atom, or which contains an imino group and an oxygen, sulphur or nitrogen atom, or R.sub.5 represents a 6-membered heteroaromatic ring bound via a carbon atom which contains 1 or 2 nitrogen atoms, whilst both the 5-membered and the 6-membered heteroaromatic rings are each attached, via two adjacent carbon atoms to an n-propylene, n-butylene or 1,3-butadienyl group or are each attached via an imino group and an adjacent carbon atom to an n-propylene, n-butylene or 1,3-butadienyl group and, in an anellated pyridine ring thus formed, a methine group may be replaced by a nitrogen atom and a vinylene group in the 3-, 4-position relative to the nitrogen atom of the pyridine ring formed may be replaced by a sulphur atom or, in an anellated phenyl ring thus formed, one or two methine groups may be replaced by N-atoms, whilst additionally the above-mentioned aromatic or heteroaromatic fused rings may be monosubstituted in the carbon structure by a fluorine, chlorine or bromine atom, by an alkyl, alkoxy, hydroxy, phenyl, nitro, amino, alkylamino, dialkylamino, alkanoylamino, cyano, carboxy, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, trifluoromethyl, alkanoyl, aminosulphonyl, alkylamino-sulphonyl or dialkylaminosulphonyl group or may be disubstituted by fluorine or chlorine atoms or by methyl, methoxy or hydroxy groups, and any -NH- group present in an imidazole ring may be substituted by a C.sub.1-6 -alkyl group or by a cycloalkyl group,
a pyrrolidine, piperidine or pyridine ring bound via a carbon atom, wherein a phenyl group may be fused onto the pyridine ring via two adjacent carbon atoms and a methylene group adjacent to the N-atom in a pyrrolidine or piperidine ring may be replaced by a carbonyl group,
an imidazolidinedione group optionally substituted by an alkyl, phenylalkyl, tetramethylene, pentamethylene or hexamethylene group,
a pyridazin-3-one or dihydro-pyridazin-3-one group which may be substituted in the 2-position by an optionally phenyl-substituted alkyl group, and in the carbon structure may additionally be substituted by 1 or 2 alkyl groups, or
an R.sub.11 --NR.sub.10 --CO--NR.sub.9 group, wherein
R.sub.9 represents a hydrogen atom, a C.sub.1-8 -alkyl group or a phenylalkyl group,
R.sub.10 represents a hydrogen atom, a C.sub.1-8 -alkyl group, a C.sub.3-5 -alkenyl group, a phenylalkyl group or a C.sub.5-7 -cycloalkyl group,
R.sub.11 represents a hydrogen atom or a C.sub.1-6 -alkyl group or
one of the groups R.sub.9, R.sub.10 or R.sub.11 may also represent a bicyclohexyl or biphenylyl group or
R.sub.10 and R.sub.11 together with the nitrogen atom between them represent a straight-chained C.sub.4-6 -alkyleneimino group or a morpholino group or
R.sub.9 and R.sub.11 together represent a C.sub.2-4 -alkylene group,
R.sub.b represents a cyano, trifluoromethylcarbonylamino, trifluoromethylcarbonylaminomethyl, trifluoromethylsulphonylamino, trifluoromethylsulphonylaminomethyl, alkylsulphonylamino, alkylsulphonylaminomethyl, arylsulphonylamino, arylsulphonylaminomethyl, aralkylsulphonylamino, aralkylsulphonylaminomethyl, arylsulphonylaminocarbonyl, benzylsulphonylamino-carbonyl, sulpho, aminosulphonyl, alkylaminosulphonyl, aralkylaminosulphonyl, arylaminosulphonyl, alkylcarbonylaminosulphonyl, aralkylcarbonylamino-sulphonyl, arylcarbonylaminosulphonyl, sulphomethyl, aminosulphonylmethyl, alkylaminosulphonylmethyl, aralkylaminosulphonylaminomethyl, arylaminosulphonyl-methyl, alkylcarbonylaminosulphonylmethyl, aralkyl-carbonylaminosulphonylmethyl, arylcarbonylaminosulphonylmethyl, phosphino, O-alkyl-phosphino, O-aralkylphosphino, O-aryl-phosphino, phosphono, O-alkyl-phosphono, O-aralkyl-phosphono, O-aryl-phosphono, OO-dialkylphosphono, phosphono-methyl, O-alkyl-phosphono-methyl, O-aralkyl-phosphonomethyl, O-aryl-phosphono-methyl, OO-dialkylphosphono-methyl, phosphato, O-alkyl-phosphato, O-aralkyl-phosphato, O-arylphosphato or OO-dialkoxy-phosphoryl group, a 1H-tetrazolyl, 1H-tetrazolylalkyl, 1H-tetrazolylaminocarbonyl or triazolyl group optionally substituted by an alkyl, trifluoromethyl, phenylalkyl or triphenylmethyl group, or an (C.sub.1-6 alkyl)sulphonylaminocarbonyl or perfluoro-(C.sub.1-6 alkyl)-sulphonylaminocarbonyl group, a carboxy group, a group which is metabolically converted into a carboxy group in vivo, or an aralkoxycarbonyl group,
R.sub.c represents a hydrogen atom, an alkyl, aralkyl, aryl, carboxy or alkoxycarbonyl group,
R.sub.d represents a straight-chained or branched C.sub.1-10 -alkyl chain, a straight-chained or branched C.sub.2-10 -alkenyl or C.sub.2-10 -alkynyl group, a cycloalkyl or cycloalkylalkyl group, a phenyl group optionally mono- or di-substituted by fluorine, chlorine or bromine atoms or by methyl or methoxy groups, or a biphenyl, naphthyl or heteroaryl group,
R.sub.e and R.sub.f represent hydrogen atoms and if R.sub.5 represents a phthalimino, homophthalimino, 2-carboxyphenylcarbonyl-amino or 2-carboxyphenylmethylamino group, wherein a carbonyl group in a phthalimino group may be replaced by a methylene, alkyl-methylene or dialkyl-methylene group and a methylene group in a homophthalimino group may be substituted by one or two alkyl groups, wherein the phenyl nuclei in any of said groups may be additionally mono- or disubstituted by alkyl or alkoxy groups and may be additionally wholly or partially hydrogenated, in which the substituents may be the same or different,
a carboxy group or a group which is metabolically converted into a carboxy group in vivo,
a 5-, 6- or 7-membered alkyleneimino or alkenyleneimino group optionally substituted by one or two alkyl groups or by a tetramethylene or pentamethylene group, wherein a methylene group of the alkyleneimino or alkenyleneimino group may be replaced by a carbonyl or sulphonyl group,
a bicycloalkane-2,3-dicarboxylic acid imino or bicycloalkene-2,3-dicarboxylic acid imino group, wherein the bicycloalkane and bicycloalkene moieties may each contain 9 or 10 carbon atoms and may be substituted by 1, 2 or 3 methyl groups and an endomethylene group may be replaced by an oxygen atom,
a glutaric acid imino group wherein the n-propylene group may be perfluorinated or substituted by one or two alkyl groups or by a tetramethylene or pentamethylene group,
a maleic acid imido or succinimido group optionally mono- or disubstituted by an alkyl or phenyl group, in which the substituents may be identical or different,
a 5-membered heteroaromatic ring bound via a carbon atom or via an imino group and containing an imino group, an oxygen or sulphur atom or containing an imino group and an oxygen, sulphur or nitrogen atom, or a 6-membered heteroaromatic ring bound via a carbon atom and containing 1 or 2 nitrogen atoms, wherein both the 5-membered and the 6-membered heteroaromatic rings are each attached, via two adjacent carbon atoms to an n-propylene, n-butylene or 1,3-butadienyl group, or are each attached via an imino group and an adjacent carbon atom to an n-propylene, n-butylene or 1,3-butadienyl group, and in the anellated pyridine ring thus formed, a methine group may be replaced by a nitrogen atom and a vinylene ring in the 3-, 4-position relative to the nitrogen atom of the pyridine ring formed may be replaced by a sulphur atom or, in an anellated phenyl ring thus formed, one or two methine groups may be replaced by N-atoms, whilst additionally the above-mentioned aromatic or heteroaromatic fused rings may be monosubstituted in the carbon skeleton by a fluorine, chlorine or bromine atom or by an alkyl, alkoxy, hydroxy, phenyl, nitro, amino, alkylamino, dialkylamino, alkanoylamino, cyano, carboxy, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, trifluoromethyl, alkanoyl, aminosulphonyl, alkylaminosulphonyl or dialkylaminosulphonyl group or may be disubstituted by fluorine or chlorine atoms or by methyl, methoxy or hydroxy groups and any -NH- group present in an imidazole ring may be substituted by a C.sub.1-6 -alkyl group or by a cycloalkyl group, or
a pyrrolidine, piperidine or pyridine ring bound via a carbon atom, in which a phenyl group may be condensed onto the pyridine ring via two adjacent carbon atoms and a methylene group adjacent to the N-atom in a pyrrolidine or piperidine ring may be replaced by a carbonyl group,
an imidazolidinedione group optionally substituted by an alkyl, phenylalkyl, tetramethylene, pentamethylene or hexamethylene group,
a pyridazin-3-one or dihydro-pyridazin-3-one group which may be substituted in the 2-position by an optionally phenyl-substituted alkyl group and may additionally be substituted in the carbon skeleton by 1 or 2 alkyl groups, or
an R.sub.11 --NR.sub.10 --CO--NR.sub.9 group, wherein R.sub.9, R.sub.10 and R.sub.11 are defined as hereinbefore,
then R.sub.e may also represent a hydrogen, fluorine, chlorine or bromine atom or an alkyl or alkoxy group and
R.sub.f may also represent a fluorine, chlorine or bromine atom or an alkoxy group,
especially those compounds wherein R.sub.a to R.sub.f, A, B and n are as hereinbefore defined with the proviso that
D.sub.4 to D.sub.7 represent methine groups with the additional provisos (a) to (j) or
D.sub.7 represents a nitrogen atom and the groups D.sub.4, D.sub.5 and D.sub.6 represent methine groups with the additional provisos (a) to (g) and (k) to (m) or (a) to (g) and (n) to (p) or
one of the groups D.sub.4, D.sub.5 or D.sub.6 represents a nitrogen atom and the remaining groups of groups D.sub.4 to D.sub.6 and D.sub.7 represent methine groups or
two of the groups D.sub.4 to D.sub.7 represent nitrogen atoms and the remaining groups of groups D.sub.4 to D.sub.7 represent methine groups, with the additional provisos that either
(a) n represents the number 1 or
(b) E has the meanings given for E hereinbefore with the exception of the carbon-carbon bond or
(c) A has the meanings given for A hereinbefore with the exception of the methylene group or
(d) B has the meanings for B given hereinbefore with the exception of the oxygen atom or
(e) R.sub.b has the meanings given for R.sub.b hereinbefore with the exception of the carboxyl group or
(f) R.sub.c has the meanings given for R.sub.c hereinbefore with the exception of the hydrogen atom or
(g) R.sub.d has the meanings given for R.sub.d hereinbefore with the exception of the phenyl group or
(h) R.sub.1 has the meanings given for R.sub.1 hereinbefore with the exception of the n-butyl group or
(i) R.sub.4 has the meanings given for R.sub.4 hereinbefore with the exception of the methyl group in position 7 or
(j) R.sub.5 has the meanings given for R.sub.5 hereinbefore with the exception of the hydrogen atom or
(k) R.sub.1 has the meanings given for R.sub.1 hereinbefore with the exception of the ethyl group or
(l) R.sub.4 has the meanings given for R.sub.4 hereinbefore with the exception of the methyl group in position 7 or
(m) R.sub.5 has the meanings given for R.sub.5 hereinbefore with the exception of the methyl group in position 5 or
(n) R.sub.1 has the meanings given for R.sub.1 hereinbefore with the exception of the n-propyl group or
(o) R.sub.4 has the meanings given for R.sub.4 hereinbefore with the exception of the hydrogen atom or
(p) R.sub.5 has the meanings given for R.sub.5 hereinbefore with the exception of the hydrogen atom and
the other groups have the meanings given hereinbefore,
whilst additionally a methine group mentioned in the above definitions of groups D.sub.4 to D.sub.7 may be substituted by a group R.sub.4 and a further methine group mentioned in the above definitions of groups D.sub.4 to D.sub.7 may be substituted by a group R.sub.5,
whilst, unless otherwise stated,
the above-mentioned alkyl, alkylene or alkoxy moieties may each contain 1 to 4 carbon atoms and the cycloalkyl moieties may each contain 3 to 7 carbon atoms, and
the term "an aryl group" refers to a phenyl group optionally mono- or di-substituted by a fluorine, chlorine or bromine atom or by a hydroxy, alkyl, alkoxy, phenylalkoxy, phenyl, nitro, amino, alkylamino, dialkylamino, alkanoylamino, cyano, carboxy, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, trifluoromethyl, alkanoyl, aminosulphonyl, alkylaminosulphonyl or dialkylamino-sulphonyl group, wherein the alkyl moiety may contain 1 to 4 carbon atoms in each case, or to a naphthyl group,
the term "a heteroaryl group" refers to a 5-membered heteroaromatic ring which contains an imino group, an oxygen or sulphur atom, or contains an imino group and an oxygen, sulphur or nitrogen atom, or contains an imino group and 2 or 3 nitrogen atoms, and a 6-membered heteroaromatic ring which contains 1, 2 or 3 nitrogen atoms, whilst the above-mentioned rings may additionally be mono- or di-substituted by a fluorine, chlorine or bromine atom or by an alkyl, alkoxy, hydroxy, phenyl, nitro, amino, alkylamino, dialkylamino, alkanoylamino, cyano, carboxy, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, trifluoromethyl, alkanoyl, aminosulphonyl, alkylaminosulphonyl or dialkylamino-sulphonyl group, and
the above-mentioned phrase "a group which is metabolically converted into a carboxy group in vivo" refers, for example, to the esters of formulae
--CO--OR',
--CO--O--(HCR")--O--CO--R"' and
--CO--O--(HCR")--O--CO--OR"'
wherein
R' represents a straight-chained or branched C.sub.1-6 -alkyl group, a C.sub.5-7 -cycloalkyl group, a benzyl, 1-phenylethyl, 2-phenylethyl, 3-phenylpropyl, pivaloyloxymethyl, phthalidylmethyl, (1,3-dioxa-2-oxo-4-methyl-cyclopenten-5-yl)-methyl, methoxymethyl or cinnamyl group,
R" represents a hydrogen atom or a methyl group and
R"' represents a straight-chained or branched C.sub.1-6 -alkyl group, a C.sub.5-7 -cycloalkyl group, a phenyl, benzyl, 1-phenylethyl, 2-phenylethyl or 3-phenylpropyl group.
The new compounds of general formula I above wherein R.sub.a represents a chlorine or bromine atom, a hydroxy, alkylsulphonyloxy, phenylsulphonyloxy or phenylalkylsulphonyloxy group, are valuable intermediate products and the other compounds of general formula I above and the physiologically acceptable salts thereof have valuable pharmacological properties, being angiotensin-antagonists, especially angiotensin-II-antagonists.
The present invention thus relates to the new above-mentioned phenylalkyl derivatives, whilst the corresponding O-substituted phosphono or phosphato compounds and the corresponding cyano, alkoxycarbonyl or triphenylmethyl compounds are also valuable intermediate products.
The present invention thus also relates to new pharmaceutical compositions containing one of the above-mentioned pharmacologically active compounds of general formula I or a corresponding physiologically acceptable addition salt and are particularly suitable for treating hypertension and cardiac insufficiency, also for treating ischaemic peripheral circulatory disorders, myocardial ischaemia (angina), for preventing the progression of cardiac insufficiency after myocardial infarction, for treating diabetic nepthropathy, glaucoma, gastrointestinal disorders and diseases of the bladder.
Examples of the heteroaromatic groups mentioned hereinbefore in the definitions of groups D.sub.4, D.sub.5, D.sub.6 and D.sub.7 include the pyrido, pyrimido, pyrazino or pyridazino groups which may be substituted in the carbon skeleton by the groups R.sub.4 and R.sub.5.
Preferred compounds of general formula I above are, with the exception of
(i) 2-n-butyl-1-[4-[(.alpha.-carboxy)benzyloxy]-benzyl]-benzimidazole,
(ii) 2-n-propyl-7-methyl-3-[4-[(.alpha.-carboxy)benzyloxy]-benzyl]imidazo[4,5-b]pyridine and
(iii) 5,7-dimethyl-2-ethyl-3-[4-[(.alpha.-carboxy)benzyloxy]-benzyl]imidazo[4,5-b]pyridine
those wherein
n represents the number 0 or 1,
A represents a straight-chained or branched alkylene group
B represents an oxygen atom, a straight-chained or branched C.sub.1-3 -alkylene group or an imino group optionally substituted by an alkyl group or by a C.sub.1-4 -alkanoyl group,
R.sub.a represents a group of the formula ##STR4## wherein none, one or two of the groups D.sub.4, D.sub.5, D.sub.6 or D.sub.7 represents a nitrogen atom and the remaining groups D.sub.4, D.sub.5, D.sub.6 or D.sub.7 each represent methine groups, whilst additionally a methine group may be substituted by the group R.sub.4 and a further methine group may be substituted by the group R.sub.5,
E represents a carbon-carbon bond, an oxygen or sulphur atom or an imino group optionally substituted by a C.sub.1-4 -alkyl group,
X represents an oxygen or sulphur atom,
R.sub.1 represents a straight-chained or branched C.sub.1-6 -alkyl group, a straight-chained or branched C.sub.2-6 -alkenyl or C.sub.2-6 -alkynyl group, whilst the above-mentioned saturated and unsaturated alkyl moieties may each be substituted by a fluorine, chlorine or bromine atom or by a hydroxy or amino group, or a C.sub.3-6 -cycloalkyl group,
R.sub.2 represents a hydrogen, fluorine, chlorine or bromine atom, a C.sub.1-5 -alkyl or C.sub.1-5 -perfluoroalkyl group or a cyano or nitro group,
R.sub.3 represents a hydrogen atom, a cyano group, a C.sub.1-3 -alkyl group optionally substituted by a hydroxy or alkoxy group, a C.sub.1-3 -perfluoroalkyl group, a C.sub.3-6 -alkenyl group optionally substituted by fluorine atoms, a C.sub.1-3 -alkyl group which is substituted in the terminal position by an R.sub.6 COO--, R.sub.7 S--, R.sub.7 SO--, R.sub.7 SO.sub.2 --, R.sub.7 CO--, R.sub.7 NHCOO--, R.sub.7 NHCO--, R.sub.7 NHCONR.sub.7 --, R.sub.8 CONR.sub.7 - or R.sub.8 SO.sub.2 NR.sub.7 -group, wherein
R.sub.6 represents a C.sub.1-4 -alkyl or C.sub.1-4 -perfluoroalkyl group or a cycloalkyl, phenyl, benzyl or phenylethyl group,
R.sub.7 represents a hydrogen atom or has the meanings given for R.sub.6 hereinbefore,
R.sub.8 has the meanings given for R.sub.7 hereinbefore,
R.sub.4 represents a hydrogen, fluorine, chlorine or bromine atom, a trifluoromethyl group, a cycloalkyl group, a C.sub.1-6 -alkyl group optionally substituted by a hydroxy, alkoxy or alkoxycarbonyl group and R.sub.5 represents a hydrogen, fluorine, chlorine or bromine atom,
a straight-chained or branched C.sub.1-6 -alkyl or C.sub.1-6 -perfluoroalkyl group, a C.sub.2-6 -alkenyl or C.sub.2-6 -alkynyl group, whilst the above-mentioned alkyl and alkenyl moieties may each be mono- or di-substituted by a heteroaryl, hydroxy, alkoxy, amino, alkylamino, dialkylamino, alkylcarbonylamino, N-alkyl-alkylcarbonylamino, carboxy, alkoxycarbonyl, alkylcarbonyloxy, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl or tetrazol-5-yl group,
a C.sub.1-5 -alkoxy group or an .alpha.-(1H-tetrazol-5-yl)-benzyloxy group,
a carboxy group or a group which is metabolically converted into a carboxy group in vivo,
a C.sub.1-4 -alkylsulphonyloxy group or a benzenesulphonyloxy or phenylalkylsulphonyloxy group,
an acylamino group optionally substituted at the nitrogen atom by a C.sub.1-6 -alkyl group or by a phenyl, cycloalkyl, phenylalkyl or cycloalkylalkyl group, in which the acyl group is a C.sub.1-5 -alkanoyl group, an alkoxycarbonyl group with a total of 2 to 4 carbon atoms, a C.sub.1-6 -alkylsulphonyl group, a benzoyl, benzenesulphonyl, cycloalkylcarbonyl, phenylalkanoyl or cycloalkylalkanoyl group, whilst the above-mentioned phenyl nuclei may each be mono- or di-substituted by a fluorine, chlorine or bromine atom or by a methyl or methoxy group and the substituents may be identical or different,
a phthalimino or homophthalimino group, wherein a carbonyl group in a phthalimino group may be replaced by a methylene, alkyl-methylene or dialkyl-methylene group, and a methylene group in a homophthalimino group may be substituted by one or two alkyl groups, wherein the phenyl nuclei in any of said groups may be additionally mono- or disubstituted by alkyl or alkoxy groups and may be additionally wholly or partially hydrogenated, in which the substituents may be the same or different,
a 5-, 6- or 7-membered alkyleneimino group optionally substituted by one or two alkyl groups or by a tetramethylene or pentamethylene group, wherein a methylene group of the alkyleneimino group may be replaced by a carbonyl or sulphonyl group,
a maleic acid imido or succinimido group optionally mono- or disubstituted by an alkyl or phenyl group, wherein the substituents may be identical or different,
a 5-membered heteroaromatic ring bound via a carbon atom or via an imino group, which contains an imino group, an oxygen or sulphur atom or which contains an imino group and an oxygen, sulphur or nitrogen atom, or a 6-membered heteroaromatic ring bound via a carbon atom which contains 1 or 2 nitrogen atoms, whilst both the 5-membered and the 6-membered heteroaromatic rings are each connected, via two adjacent carbon atoms to an n-propylene, n-butylene or 1,3-butadienyl group or are each attached via an imino group and an adjacent carbon atom to an n-propylene, n-butylene or 1,3-butadienyl group and, in an anellated pyridine ring thus formed, a methine group may be replaced by a nitrogen atom and a vinylene group in the 3-, 4-position relative to the nitrogen atom of the pyridine ring formed may be replaced by a sulphur atom or, in an anellated phenyl ring thus formed, one or two methine groups may be replaced by N-atoms, whilst additionally the above-mentioned aromatic or heteroaromatic fused rings may be monosubstituted in the carbon structure by a fluorine, chlorine or bromine atom or by an alkyl, alkoxy, hydroxy, phenyl, nitro, amino, alkylamino, dialkylamino, alkanoylamino, cyano, carboxy, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, trifluoromethyl, alkanoyl, aminosulphonyl, alkylamino-sulphonyl or dialkylaminosulphonyl group or may be disubstituted by fluorine or chlorine atoms or by methyl, methoxy or hydroxy groups, and any -NH- group present in an imidazole ring may be substituted by a C.sub.1-6 -alkyl group, or
an R.sub.11 --NR.sub.10 --CO--NR.sub.9 group wherein
R.sub.9 represents a hydrogen atom, a C.sub.1-6 -alkyl group or a phenylalkyl group,
R.sub.10 represents a hydrogen atom, a C.sub.1-6 -alkyl group, a phenylalkyl group or a C.sub.5-7 -cycloalkyl group,
R.sub.11 represents a hydrogen atom or a C.sub.1-6 -alkyl group or
R.sub.10 and R.sub.11 together with the nitrogen atom between them represent a straight-chained C.sub.4-6 -alkyleneimino group or a morpholino group or
R.sub.9 and R.sub.11 together represent a C.sub.2-4 -alkylene group,
R.sub.b represents a cyano, carboxy, arylsulphonylamino-carbonyl, benzylsulphonylaminocarbonyl, sulpho, alkylcarbonylaminosulphonyl, aralkylcarbonylamino-sulphonyl, arylcarbonylaminosulphonyl, alkylcarbonyl-aminosulphonylmethyl, aralkylcarbonylaminosulphonyl-methyl, arylcarbonylaminosulphonylmethyl, phosphino, O-alkyl-phosphino, phosphono, O-alkyl-phosphono, OO-dialkylphosphono, phosphonomethyl, O-alkyl-phosphono-methyl, OO-dialkylphosphono-methyl, phosphato or O-alkyl-phosphato group, a 1-H-tetrazolyl or 1H-tetrazolyl-alkyl group optionally substituted by a phenylalkyl or triphenylmethyl group, an (C.sub.14 6 alkyl)sulphonylamino-carbonyl or perfluoro(C.sub.1-6 alkyl)sulphonylaminocarbonyl, a group which is metabolically converted into a carboxy group in vivo, or an aralkoxycarbonyl group,
R.sub.c represents a hydrogen atom, a methyl, phenyl, benzyl, carboxy or alkoxycarbonyl group and
R.sub.d represents a straight-chained or branched C.sub.1-6 -alkyl group, a straight-chained or branched C.sub.2-6 -alkenyl or C.sub.2-6 alkynyl group, a cycloalkyl or cycloalkylalkyl group, a phenyl group optionally mono- or disubstituted by fluorine, chlorine or bromine atoms or by methyl or methoxy groups, or a biphenyl, naphthyl or heteroaryl group,
R.sub.e and R.sub.f represent a hydrogen atom and, if
R.sub.5 represents a phthalimino or homophthalimino group, wherein a carbonyl group in a phthalimino group may be replaced by a methylene, alkylmethylene or dialkylmethylene group and a methylene group in a homophthalimino group may be substituted by one or two alkyl groups, wherein the phenyl nuclei in any of said groups may be additionally mono- or disubstituted by alkyl or alkoxy groups and may be additionally wholly or partially hydrogenated, in which the substituents may be the same or different,
a carboxy group or a group which is metabolically converted into a carboxy group in vivo,
a 5-, 6- or 7-membered alkyleneimino group optionally substituted by one or two alkyl groups or by a tetramethylene or pentamethylene group, wherein a methylene group of the alkyleneimino group may be replaced by a carbonyl or sulphonyl group,
a maleic acid imido or succinimido group optionally mono- or di-substituted by an alkyl or phenyl group, the substituents being identical or different,
a 5-membered heteroaromatic ring bound via a carbon atom or via an imino group, which contains an imino group, an oxygen or sulphur atom or which contains an imino group and an oxygen, sulphur or nitrogen atom, or a 6-membered heteroaromatic ring bound via a carbon atom which contains 1 or 2 nitrogen atoms, whilst both the 5-membered and the 6-membered heteroaromatic rings are each attached via two adjacent carbon atoms to an n-propylene, n-butylene or 1,3-butadienyl group or are each attached via an imino group and an adjacent carbon atom to an n-propylene, n-butylene or 1,3-butadienyl group and, in an anellated pyridine ring thus formed, a methine group may be replaced by a nitrogen atom and a vinylene group in the 3-, 4-position relative to the nitrogen atom of the pyridine ring formed may be replaced by a sulphur atom or, in an anellated phenyl ring thus formed, one or two methine groups may be replaced by N-atoms, whilst additionally the above-mentioned aromatic or heteroaromatic fused rings may be monosubstituted in the carbon structure by a fluorine, chlorine or bromine atom or by an alkyl, alkoxy, hydroxy, phenyl, nitro, amino, alkylamino, dialkylamino, alkanoylamino, cyano, carboxy, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, trifluoromethyl, alkanoyl, aminosulphonyl, alkylamino-sulphonyl or dialkylaminosulphonyl group or may be disubstituted by fluorine or chlorine atoms or by methyl, methoxy or hydroxy groups, and any -NH- group present in an imidazole ring may be substituted by a C.sub.1-6 -alkyl group, or
an R.sub.11 --NR.sub.10 --CO--NR.sub.9 group wherein R.sub.9 to R.sub.11 are defined as hereinbefore,
then R.sub.e may also represent a hydrogen, fluorine, chlorine or bromine atom or an alkyl or alkoxy group and
R.sub.f may also represent a fluorine, chlorine or bromine atom or an alkoxy group,
whilst unless otherwise specified the expression used hereinbefore "a group which is converted metabolically into a carboxy group in vivo" is defined as hereinbefore,
the above-mentioned alkyl, alkylene or alkoxy moieties may each contain 1 to 4 carbon atoms and the cycloalkyl moieties may each contain 3 to 7 carbon atoms, and
the expression "an aryl group" denotes a phenyl group optionally mono- or disubstituted by a fluorine, chlorine or bromine atom or by a hydroxy, alkyl, alkoxy, phenylalkoxy, phenyl or nitro group, wherein each alkyl moiety may contain from 1 to 4 carbon atoms, or a naphthyl group and
"a heteroaryl group" denotes a 5-membered heteroaromatic ring which contains an imino group, an oxygen or sulphur atom or which contains an imino group and an oxygen, sulphur or nitrogen atom or which contains an imino group and 2 or 3 nitrogen atoms, and a 6-membered heteroaromatic ring which contains 1, 2 or 3 nitrogen atoms, whilst the above-mentioned rings may additionally be mono-or disubstituted by a fluorine, chlorine or bromine atom or by an alkyl, alkoxy, hydroxy, phenyl or nitro group,
the isomer mixtures thereof, the tautomers, enantiomers and salts thereof.
Particularly preferred compounds of general formula I above are, with the exception of
(i) 2-n-butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-benzimidazole,
(ii) 2-n-propyl-7-methyl-3-[4-[(.alpha.-carboxy)benzyloxy]-benzyl]imidazo[4,5-b]pyridine and
(iii) 5,7-dimethyl-2-ethyl-3-[4-[(.alpha.-carboxy)benzyloxy]-benzyl]imidazo[4,5-b]pyridine,
those wherein
n represents the number 0,
A denotes a methylene, ethylene or ethylidene group,
B denotes an oxygen atom, a methylene, imino, methylimino or acetylimino group,
R.sub.a denotes a group of the formula ##STR5## wherein none, one or two of the groups D.sub.4, D.sub.5, D.sub.6 or D.sub.7 represents a nitrogen atom and the remaining groups D.sub.4, D.sub.5, D.sub.6 or D.sub.7 each represent methine groups, whilst additionally a methine group may be substituted by a group R.sub.4 and another methine group may be substituted by a group R.sub.5,
E represents an oxygen atom or a carbon-carbon bond,
R.sub.1 represents a straight-chained or branched C.sub.1-6 -alkyl group,
R.sub.2 represents a hydrogen, fluorine, chlorine or bromine atom,
R.sub.3 represents a hydroxyalkyl group having 1 or 2 carbon atoms,
R.sub.4 represents a hydrogen atom or a C.sub.1-3 -alkyl group and
R.sub.5 represents a hydrogen, fluorine, chlorine or bromine atom,
a straight-chained or branched C.sub.1-3 -alkyl group or an .alpha.-(1H-tetrazol-5-yl)-benzyloxy group,
an amino or nitro group,
a carboxy group or an alkoxycarbonyl group with a total of 2 to 4 carbon atoms,
an acylamino group optionally substituted at the nitrogen atom by a C.sub.1-5 -alkyl group, wherein the acyl group is a C.sub.1-5 -alkanoyl group or a benzenesulphonyl group, the above-mentioned phenyl nucleus optionally being mono- or di-substituted by a methyl or methoxy group and the substituents being identical or different,
a 5-, 6- or 7-membered alkyleneimino group optionally substituted by one or two methyl groups, wherein a methylene group of the alkyleneimino group may be replaced by a carbonyl or sulphonyl group, or a 2,3-dimethylsuccinimido, phthalimino, homophthalimino or isoindolin-1-on-yl group,
a benzimidazol-2-yl group optionally substituted in the 1-position by a C.sub.1-3 -alkyl group or
an R.sub.11 --NR.sub.10 --CO--NR.sub.9 group wherein
R.sub.9 represents a hydrogen atom, a C.sub.1-5 -alkyl group or a phenylalkyl group having 1 to 3 carbon atoms in the alkyl moiety,
R.sub.10 represents a hydrogen atom, a C.sub.1-5 -alkyl group or a cyclohexyl group,
R.sub.11 represents a hydrogen atom, a benzyl group or a C.sub.1-5 -alkyl group or
R.sub.9 and R.sub.11 together represent a C.sub.2-3 -alkylene group,
R.sub.b represents a carboxy, 1H-tetrazolyl or O-alkyl-phosphono or alkylsulphonylaminocarbonyl group having 1 to 3 carbon atoms in the alkyl moiety,
R.sub.c represents a hydrogen atom or a phenyl group,
R.sub.d represents a straight-chained or branched C.sub.1-4 -alkyl group, a cyclohexyl, cyclohexylmethyl, phenyl, biphenyl, methoxyphenyl, chlorophenyl, pyridyl or naphthyl group,
R.sub.e and R.sub.f represent hydrogen atoms and, if
R.sub.5 represents a benzimidazol-2-yl group optionally substituted in the 1-position by a C.sub.1-3 -alkyl group,
a 2,3-dimethylsuccinimino group,
a 5-, 6- or 7-membered alkyleneimino group optionally substituted by one or two methyl groups, wherein a methylene group may be replaced by a carbonyl or sulphonyl group, or
an R.sub.11 --NR.sub.10 --CO--NR.sub.9 group, wherein R.sub.9 to R.sub.11 are defined as hereinbefore,
then R.sub.e may also represent a hydrogen, chlorine or bromine atom or a methoxy group and
R.sub.f may also represent a chlorine or bromine atom or a methoxy group,
the isomer mixtures thereof, the tautomers, enantiomers and salts thereof.
According to the invention, the compounds of general formula I are obtained by the following methods:
a) reacting a compound of general formula
R.sub.a --H (II)
wherein R.sub.a is as hereinbefore defined, with a compound of general formula ##STR6## wherein A, B, n and R.sub.c to R.sub.f are as hereinbefore defined and Z.sub.1 represents a nucleophilic leaving group such as a halogen atom, e.g. a chlorine, bromine or iodine atom, or a substituted sulphonyloxy group, e.g. a methanesulphonyloxy, phenylsulphonyloxy or p-toluene-sulphonyloxy group, followed by hydrolysis if required.
The reaction is conveniently carried out in a solvent or mixture of solvents such as methylene chloride, diethylether, tetrahydrofuran, dioxane, dimethylsulphoxide, dimethylformamide or benzene, optionally in the presence of an acid binding agent such as sodium carbonate, potassium carbonate, sodium hydroxide, potassium tert.-butoxide, triethylamine or pyridine, whilst the latter two may simultaneously also be used as solvent, preferably at temperatures between 0.degree. and 100.degree. C., e.g. at temperatures between ambient temperature and 50.degree. C.
The subsequent hydrolysis is appropriately carried out either in the presence of an acid such as hydrochloric acid, sulphuric acid, phosphoric acid, trichloroacetic acid or trifluoroacetic acid or in the presence of a base such as sodium hydroxide or potassium hydroxide in a suitable solvent such as water, water/methanol, ethanol, water/ethanol, water/isopropanol or water/dioxane at temperatures between -10.degree. C. and 120.degree. C., e.g. at temperatures between ambient temperature and the boiling temperature of the reaction mixture.
In the reaction, a mixture of possible isomers is preferably obtained which may subsequently, if desired, be resolved into the corresponding isomers, preferably by chromatography, using a carrier such as silica gel or aluminium oxide.
b) Reacting a compound of general formula ##STR7## wherein R.sub.a, R.sub.e, R.sub.f and A are defined as hereinbefore,
B' represents a straight-chained or branched C.sub.1-4 -alkylene group and
Z.sub.2 represents a nucleophilic leaving group such as a halogen atom, e.g. a chlorine, bromine or iodine atom, or a substituted sulphonyloxy group, e.g. a methanesulphonyloxy, phenylsulphonyloxy or p-toluenesulphonyloxy group, with a compound of general formula ##STR8## wherein R.sub.b, R.sub.d and n are as hereinbefore defined and
R.sub.c ' represents an alkoxycarbonyl group, if necessary with subsequent hydrolysis and/or decarboxylation.
The reaction is conveniently carried out in a solvent or mixture of solvents such as methylene chloride, diethylether, tetrahydrofuran, dioxane, dimethylsulphoxide, dimethylformamide or benzene, optionally in the presence of an acid binding agent such as sodium carbonate, potassium carbonate, sodium hydroxide, potassium tert.-butoxide, triethylamine or pyridine, whilst the latter two may simultaneously also be used as solvent, preferably at temperatures between 0.degree. and 100.degree. C., e.g. at temperatures between ambient temperature and 50.degree. C.
The subsequent hydrolysis is appropriately carried out either in the presence of an acid such as hydrochloric acid, sulphuric acid, phosphoric acid, trichloroacetic acid or trifluoroacetic acid or in the presence of a base such as sodium hydroxide or potassium hydroxide in a suitable solvent such as water, water/methanol, ethanol, water/ethanol, water/isopropanol or water/dioxane at temperatures between -10.degree. C. and 120.degree. C., e.g. at temperatures between ambient temperature and the boiling temperature of the reaction mixture.
The subsequent decarboxylation is conveniently carried out in the presence of an acid such as hydrochloric acid, sulphuric acid, phosphoric acid, acetic acid, trichloroacetic acid or trifluoroacetic acid in a suitable solvent such as water, water/methanol, ethanol, water/ethanol, water/isopropanol or water/dioxane at temperatures between 50.degree. C. and 120.degree. C., e.g. at temperatures between 60.degree. C. and the boiling temperature of the reaction mixture.
c) In order to prepare compounds of general formula I wherein B represents an oxygen or sulphur atom or an optionally alkyl-substituted imino group:
Reacting a compound of general formula ##STR9## wherein R.sub.a, R.sub.e, R.sub.f and A are as hereinbefore defined and B" represents an oxygen or sulphur atom or an optionally alkyl-substituted imino group, with a compound of general formula ##STR10## wherein R.sub.b to R.sub.d and n are as hereinbefore defined and Z.sub.3 represents a nucleophilic leaving group such as a halogen atom, e.g. a chlorine, bromine or iodine atom, or a substituted sulphonyloxy group, e.g. a methanesulphonyloxy, phenylsulphonyloxy or p-toluene-sulphonyloxy group, if necessary with subsequent hydrolysis.
The reaction is conveniently carried out in a solvent or mixture of solvents such as methylene chloride, diethylether, tetrahydrofuran, dioxane, dimethylsulphoxide, dimethylformamide or benzene, optionally in the presence of an acid binding agent such as sodium acetate, sodium carbonate, potassium carbonate, sodium hydroxide, potassium tert.-butoxide, triethylamine or pyridine, whilst the latter two may simultaneously also be used as solvent, preferably at temperatures between 0.degree. and 100.degree. C., e.g. at temperatures between ambient temperature and the boiling temperature of the reaction mixture.
The subsequent hydrolysis is appropriately carried out either in the presence of an acid such as hydrochloric acid, sulphuric acid, phosphoric acid, trichloroacetic acid or trifluoroacetic acid or in the presence of a base such as sodium hydroxide or potassium hydroxide in a suitable solvent such as water, water/methanol, ethanol, water/ethanol, water/isopropanol or water/dioxane at temperatures between -10.degree. C. and 120.degree. C., e.g. at temperatures between ambient temperature and the boiling temperature of the reaction mixture.
d) In order to prepare a compound of general formula I wherein R.sub.b represents a carboxy group:
Converting a compound of general formula ##STR11## wherein R.sub.a, R.sub.c to R.sub.f, A, B and n are as hereinbefore defined and R.sub.b ' represents a group which may be converted into a carboxy group by hydrolysis, thermolysis or hydrogenolysis.
For example, functional derivatives of the carboxy group such as the unsubstituted or substituted amides, esters, thiolesters, orthoesters, iminoethers, amidines or anhydrides, the nitrile group or the tetrazolyl group may be converted by hydrolysis into a carboxy group, esters with tertiary alcohols, e.g. the tert.butylester, may be converted by thermolysis into a carboxy group and esters with aralkanols, e.g. the benzylester, may be converted by hydrogenolysis into a carboxy group.
The hydrolysis is appropriately carried out either in the presence of an acid such as hydrochloric acid, sulphuric acid, phosphoric acid, trichloroacetic acid or trifluoroacetic acid or in the presence of a base such as sodium hydroxide or potassium hydroxide in a suitable solvent such as water, water/methanol, ethanol, water/ethanol, water/isopropanol or water/dioxane at temperatures between -10.degree. C. and 120.degree. C., e.g. at temperatures between ambient temperature and the boiling temperature of the reaction mixture. When hydrolysis is carried out in the presence of an organic acid such as trichloroacetic acid or trifluoroacetic acid, any alcoholic hydroxy groups present may simultaneously be converted into a corresponding acyloxy group such as the trifluoroacetoxy group.
If R.sub.b ' in a compound of general formula VIII represents a cyano or aminocarbonyl group, these groups may also be converted into the carboxy group with a nitrite, e.g. sodium nitrite, in the presence of an acid such as sulphuric acid, which may simultaneously also be used as solvent, at temperatures between 0.degree. and 50.degree. C.
If R.sub.b ' in a compound of general formula VIII represents for example the tert.-butyloxycarbonyl group, the tert.-butyl group may also be thermally cleaved, optionally in an inert solvent such as methylene chloride, chloroform, benzene, toluene, tetrahydrofuran or dioxane and preferably in the presence of a catalytic amount of an acid such as trifluoroacetic acid, p-toluenesulphonic acid, sulphuric acid, phosphoric acid or polyphosphoric acid, preferably at the boiling temperature of the solvent used, e.g. at temperatures between 40.degree. C. and 100.degree. C.
If R.sub.b ' in a compound of general formula VIII represents the benzyloxycarbonyl group, for example, the benzyl group may also be hydrogenolytically cleaved in the presence of a hydrogenation catalyst such as palladium/charcoal in a suitable solvent such as methanol, ethanol, ethanol/water, glacial acetic acid, ethyl acetate, dioxane or dimethylformamide, preferably at temperatures between 0.degree. and 50.degree. C., e.g. at ambient temperature, under a hydrogen pressure of 1 to 5 bar. During hydrogenolysis, other groups may be reduced at the same time, e.g. a nitro group may be reduced to the amino group, a benzyloxy group to the hydroxy group, a vinylidene group to the corresponding alkylidene group or a cinnamic acid group to the corresponding phenylpropionic acid group, or they may be replaced by hydrogen atoms, e.g. a halogen may be replaced by a hydrogen atom.
e) In order to prepare a compound of general formula I wherein R.sub.b represents a 1H-tetrazolyl group:
Cleaving a protective group from a compound of general formula ##STR12## wherein R.sub.a, R.sub.c to R.sub.f, A, B and n are as hereinbefore defined and R.sub.b " represents a 1H-tetrazolyl group protected in the 1- or 2-position by a protecting group.
Suitable protecting groups include, for example, the triphenylmethyl, tributyl tin or triphenyl tin groups.
The cleaving of a protecting group used is preferably carried out in the presence of a hydrohalic acid, preferably in the presence of hydrochloric acid, in the presence of a base such as sodium hydroxide or alcoholic ammonia in a suitable solvent such as methylene chloride, methanol, methanol/ammonia, ethanol or isopropanol at temperatures between 0.degree. and 100.degree. C., but preferably at ambient temperature or, if the reaction is carried out in the presence of alcoholic ammonia, at elevated temperatures, e.g. at temperatures between 100.degree. and 150.degree. C., preferably at temperatures between 120.degree. and 140.degree. C.
f) In order to prepare a compound of general formula I wherein R.sub.b represents a 1H-tetrazolyl group:
Reacting a compound of general formula ##STR13## wherein R.sub.a, R.sub.c to R.sub.f, A, B and n are as hereinbefore defined, with hydrazoic acid or the salts thereof.
The reaction is preferably carried out in a solvent such as benzene, toluene or dimethylformamide at temperatures between 80.degree. and 150.degree. C., preferably at 125.degree. C. Appropriately, either the hydrazoic acid is liberated during the reaction from an alkali metal azide, e.g. sodium azide, in the presence of a weak acid such as ammonium chloride or the tetrazolide salt obtained in the reaction mixture during the reaction with a salt of hydrazoic acid, preferably with aluminium azide or tributyl tin azide, which is also preferably produced in the reaction mixture by reacting aluminium chloride or tributyl tin chloride with an alkali metal azide such as sodium azide, is subsequently liberated by acidification with a dilute acid such as 2N hydrochloric or 2N sulphuric acid.
g) In order to prepare compounds of general formula I wherein R.sub.a represents a group of the formula ##STR14## cyclising a compound of general formula ##STR15## wherein D.sub.1 to D.sub.7 are as hereinbefore defined, one of the groups X.sub.1 or Y.sub.1 represents a group of general formula ##STR16## and the other group X.sub.1 or Y.sub.1 represents a group of general formula ##STR17## wherein R.sub.1, A, B, E, n and R.sub.b to R.sub.f are as hereinbefore defined, Z.sub.4 and Z.sub.5, which may be identical or different, represent optionally substituted amino groups or hydroxy or mercapto groups optionally substituted by lower alkyl groups or Z.sub.4 and Z.sub.5 together represent an oxygen or sulphur atom, an imino group optionally substituted by a C.sub.1-3 -alkyl group, or an alkylenedioxy or alkylenedithio group each having 2 or 3 carbon atoms.
The cyclisation is conveniently carried out in a solvent or mixture of solvents such as ethanol, isopropanol, glacial acetic acid, benzene, chlorobenzene, toluene, xylene, glycol, glycol-monomethylether, diethyleneglycoldimethylether, sulpholane, dimethylformamide, tetraline or in an excess of the acylating agent used to prepare the compound of general formula XI, e.g. in the corresponding nitrile, anhydride, acid halide, ester or amide, e.g. at temperatures between 0.degree. and 250.degree. C., but preferably at the boiling temperature of the reaction mixture, optionally in the presence of a condensing agent such as phosphorusoxychloride, thionylchloride, sulphurylchloride, sulphuric acid, p-toluenesulphonic acid, methanesulphonic acid, hydrochloric acid, phosphoric acid, polyphosphoric acid, acetic anhydride or optionally in the presence of a base such as potassium ethoxide or potassium tert.-butoxide. However, cyclisation may also be carried out without a solvent and/or condensing agent.
However, it is particularly advantageous to carry out the reaction by preparing a compound of general formula XI in the reaction mixture by reducing a corresponding o-nitro-amino compound, optionally in the presence of a carboxylic acid of general formula R.sub.a --E--COOH, or by acylation of a corresponding o-diamino compound. When the reduction of the nitro group is broken off at the hydroxylamine stage, the N-oxide of a compound of general formula I is obtained in the subsequent cyclisation. The resulting N-oxide is then converted by reduction into a corresponding compound of general formula I.
The subsequent reduction of the N-oxide of formula I obtained is preferably carried out in a solvent such as water, water/ethanol, methanol, glacial acetic acid, ethyl acetate or dimethylformamide with hydrogen in the presence of a hydrogenation catalyst such as Raney nickel, platinum or palladium/charcoal, with metals such as iron, tin or zinc in the presence of an acid such as acetic acid, hydrochloric acid or sulphuric acid, with salts such as iron(II)sulphate, tin(II)chloride or sodium dithionite, or with hydrazine in the presence of Raney nickel at temperatures between 0.degree. and 50.degree. C., but preferably at ambient temperature.
h) In order to prepare compounds of general formula I wherein R.sub.5 represents an R.sub.11 --NR.sub.10 --CO--NR.sub.9 group:
Reacting a compound of formula ##STR18## wherein R.sub.b to R.sub.f, A, B and n are as hereinbefore defined and R.sub.a ' represents one of the groups mentioned for R.sub.a hereinbefore wherein R.sub.5 is an amino group, a C.sub.1-8 -alkylamino group or a phenyl(C.sub.1-4 alkyl)amino group, with a compound of formula ##STR19## wherein R.sub.10 represents a hydrogen atom, a C.sub.1-8 -alkyl group, a C.sub.3-5 -alkenyl group, a phenylalkyl group or a C.sub.5-7 -cycloalkyl group,
R.sub.11 represents a hydrogen atom or a C.sub.1-6 -alkyl group or one of the groups R.sub.10 or R.sub.11 also represents a bicyclohexyl or biphenylyl group or
R.sub.10 and R.sub.11 together with the nitrogen atom between them represent a straight-chained C.sub.4-6 -alkyleneimino group or a morpholino group and
Z.sub.6 represents a nucleophilic leaving group such as a chlorine or bromine atom or, if one of the groups R.sub.10 or R.sub.11 represents a hydrogen atom,
Z.sub.6 together with R.sub.10 or R.sub.11 represents a nitrogen-carbon bond.
The reaction is preferably carried out in a solvent such as tetrahydrofuran, dioxane, ethylene chloride or benzene, optionally in the presence of an acid-binding agent such as triethylamine or pyridine, expediently at temperatures between 0.degree. and 100.degree. C., preferably at temperatures between 20.degree. and 80.degree. C.
i) In order to prepare compounds of general formula I wherein R.sub.5 represents an acylamino group optionally substituted at the nitrogen atom by a C.sub.1-6 -alkyl group or by a phenyl, cycloalkyl, phenylalkyl, cycloalkylalkyl, bicyclohexyl or biphenyl group, wherein the acyl group is a C.sub.1-7 -alkanoyl group, a C.sub.2-4 -(alkoxycarbonyl) group, a C.sub.1-6 -alkylsulphonyl group, a benzoyl, benzenesulphonyl, phenylalkanesulphonyl, naphthalenesulphonyl, cycloalkylcarbonyl, phenylalkanoyl or cycloalkylalkanoyl group, whilst the above-mentioned phenyl nuclei may each be mono- or di-substituted by a fluorine, chlorine or bromine atom, by a methyl or methoxy group and the substituents may be identical or different, a phthalimino or homophthalimino group wherein the phenyl nucleus in each case may be mono- or di-substituted by C.sub.1-3 -alkyl or C.sub.1-3 -alkoxy groups, or a maleic acid imido or succinimido group optionally mono- or di-substituted by an alkyl or phenyl group, wherein the substituents may be identical or different:
Acylating a compound of formula ##STR20## wherein R.sub.b to R.sub.f, A, B and n are as hereinbefore defined and R.sub.a " represents one of the groups mentioned as a definition for R.sub.a hereinbefore, wherein R.sub.5 represents an amino group optionally substituted by a C.sub.1-6 -alkyl group or by a phenyl, cycloalkyl, phenylalkyl, cycloalkyalkyl, bicyclohexyl or biphenyl group, with a compound of formula
HO--U--R.sub.12 (XV)
wherein
U represents a carbonyl or sulphonyl group and R.sub.12 represents a C.sub.1-6 -alkyl group, a C.sub.1-3 -alkoxy group, a phenyl, phenylalkyl, naphthyl or cycloalkyl group, wherein the above-mentioned phenyl nuclei may each be mono- or disubstituted by a fluorine, chlorine or bromine atom or by a methyl or methoxy group and the substituents may be identical or different, or, if U represents a carbonyl group, R.sub.12 may also represent a hydrogen atom, a phenyl group which is substituted in the o-position by a carboxy or carboxymethyl group and wherein the phenyl nucleus may be mono- or di-substituted by C.sub.1-3 -alkyl or C.sub.1-3 -alkoxy groups and may additionally be wholly or partially hydrogenated, a 2-carboxy-ethyl or 2-carboxy-ethenyl group in which the ethyl and ethenyl moiety may each be mono- or di-substituted by an alkyl or phenyl group, or with a reactive derivative thereof such as an acid halide, acid ester or acid anhydride thereof.
Examples of reactive derivatives of a compound of formula XV include the esters thereof such as the methyl, ethyl or benzylester, the thioesters thereof such as the methylthio or ethylthioester, the halides thereof such as the acid chloride and the anhydrides or imidazolides thereof.
The reaction is conveniently carried out in a solvent or mixture of solvents such as water, methylene chloride, chloroform, ether, tetrahydrofuran, dioxane or dimethylformamide with a corresponding carboxylic acid in the presence of an acid activating or dehydrating agent such as thionylchloride, with the anhydrides thereof such as acetic acid anhydride, with the esters thereof such as ethyl acetate, with the halides thereof such as acetylchloride or methanesulphonyl chloride, optionally in the presence of an inorganic or tertiary organic base such as sodium hydroxide, potassium carbonate, triethylamine or pyridine, whilst the latter two may simultaneously also be used as solvent, at temperatures between -25.degree. and 100.degree. C., but preferably at temperatures between -10.degree. and 80.degree. C.
j) In order to prepare compounds of general formula I wherein R.sub.b represents a phosphono or O-alkyl-phosphono group:
Hydrolysis of a compound of general formula ##STR21## wherein A, B, R.sub.a, R.sub.c to R.sub.f and n are defined as hereinbefore and R.sub.b "' represents an O-alkyl- or OO-dialkyl-phosphono group in which each alkyl moiety may contain 1 to 5 carbon atoms.
The reaction is preferably carried out in a solvent such as methanol, methanol/water, ethanol or isopropanol in order to prepare a corresponding O-alkylphosphono compound of general formula I in the presence of a base such as potassium hydroxide or potassium methoxide or in order to prepare a corresponding phosphono compound of general formula I in the presence of an acid such as hydrobromic acid at the boiling temperature of the reaction mixture.
It is particularly advantageous to prepare a phosphono compound of general formula I by reacting a corresponding O-alkyl or OO-dialkyl compound of general formula XVI with bromine/trimethylchlorosilane in acetonitrile with subsequent hydrolysis using water at ambient temperature.
If according to the invention a compound of general formula I is obtained wherein R.sub.2 or R.sub.5 represents a nitro group, this may be converted by reduction into a corresponding compound of general formula I wherein R.sub.2 or R.sub.5 represents an amino group, or
if according to the invention a compound of general formula I is obtained wherein B represents an imino group, this may be converted by alkanoylation into a corresponding compound of general formula I wherein B represents an imino group substituted by a C.sub.1-4 -alkanoyl group, or
if according to the invention a compound of general formula I is obtained wherein B represents an imino group, this may be converted by alkylation into a corresponding compound of general formula I wherein B represents an imino group substituted by a C.sub.1-3 -alkyl group, or
if according to the invention a compound of general formula I is obtained wherein R.sub.b or R.sub.b and R.sub.5 each represent a carboxy group, this may be converted by esterification into a corresponding compound of general formula I wherein R.sub.b or R.sub.b and R.sub.5 represent a group which is metabolically converted in vivo into a carboxy group, or
if according to the invention a compound of general formula I is obtained wherein R.sub.b represents a carboxy group, this may, after conversion into a corresponding acid halide, be converted by reaction with a corresponding sulphonamide into a compound of general formula I wherein R.sub.b represents an alkanesulphonylaminocarbonyl, perfluoroalkanesulphonylaminocarbonyl, arylsulphonylaminocarbonyl or benzylsulphonylaminocarbonyl group.
The subsequent reduction of the nitro group is preferably carried out in a solvent such as water, water/ethanol, methanol, glacial acetic acid, ethyl acetate or dimethylformamide, suitably with hydrogen in the presence of a hydrogenation catalyst such as Raney nickel, platinum or palladium/charcoal, with metals such as iron, tin or zinc in the presence of an acid, with salts such as iron(II)sulphate, tin(II)chloride, sodium sulphide, sodium hydrogen sulphite or sodium dithionite, or with hydrazine in the presence of Raney nickel at temperatures between 0.degree. and 80.degree. C., but preferably at temperatures between 20.degree. and 40.degree. C.
The subsequent alkanoylation is suitably carried out in a solvent or mixture of solvents such as water, methylene chloride, chloroform, ether, tetrahydrofuran, dioxane or dimethylformamide with a corresponding carboxylic acid in the presence of an acid-activating or dehydrating agent such as thionylchloride, with the anhydrides thereof such as acetic anhydride, with the esters thereof such as ethyl acetate, with the halides thereof such as acetylchloride, optionally in the presence of an inorganic or tertiary organic base, such as sodium hydroxide, potassium carbonate, triethylamine or pyridine, whilst the latter two may simultaneously also be used as solvent, at temperatures between -25.degree. and 100.degree. C., but preferably at temperatures between -10.degree. and 80.degree. C.
The subsequent alkylation is suitably carried out in a solvent or mixture of solvents such as methylene chloride, diethylether, tetrahydrofuran, dioxane, dimethylsulphoxide, dimethylformamide or benzene, optionally in the presence of an acid binding agent such as sodium carbonate, potassium carbonate, sodium hydroxide, potassium tert.butoxide, triethylamine or pyridine, whilst the latter two may simultaneously also be used as solvent, with an alkylating agent such as methyliodide, ethyliodide, isopropylbromide, dimethyl-sulphate, diethylsulphate or methyl p-toluenesulphonate, preferably at temperatures between 0.degree. and 100.degree. C., e.g. at temperatures between ambient temperature and 50.degree. C.
The conversion of a carboxyl group into a group which is metabolically converted in vivo into a carboxy group, is conveniently carried out by esterification with a corresponding alcohol or with a corresponding reactive acyl derivative, suitably in a solvent or mixture of solvents such as water, methylene chloride, chloroform, ether, tetrahydrofuran, dioxane or dimethylformamide or in an excess of the acylating agent as solvent, optionally in the presence of an acid-activating or dehydrating agent such as thionylchloride, with the anhydrides, esters or halides thereof, optionally in the presence of an inorganic or tertiary organic base, such as sodium hydroxide, potassium carbonate, triethylamine or pyridine, whilst the latter two may simultaneously also be used as solvent, at temperatures between -25.degree. and 100.degree. C., but preferably at temperatures between -10.degree. and 80.degree. C.
The subsequent reaction of an acid halide with a corresponding sulphonamide is suitably carried out in a solvent such as acetone, tetrahydrofuran or dioxane in the presence of a base such as triethylamine at temperatures between 0.degree. and 50.degree. C., preferably at temperatures between 10.degree. and 30.degree. C.
In the reactions described hereinbefore, any reactive groups present such as hydroxy, amino or alkylamino groups may optionally be protected during the reaction by conventional protecting groups which are split off again after the reaction.
Examples of protecting groups for a hydroxy group are the trimethylsilyl, acetyl, benzoyl, methyl, ethyl, tert.-butyl, benzyl or tetrahydropyranyl groups and protecting groups for an amino, alkylamino or imino group include the acetyl, benzoyl, ethoxycarbonyl and benzyl groups.
The optional subsequent cleaving of a protecting group is preferably carried out by hydrolysis in an aqueous solvent, e.g. in water, isopropanol/water, tetrahydrofuran/water or dioxane/water, in the presence of an acid such as hydrochloric acid or sulphuric acid or in the presence of an alkali metal base such as sodium hydroxide or potassium hydroxide at temperatures between 0.degree. and 100.degree. C., preferably at the boiling temperature of the reaction mixture. However, a benzyl group is preferably split off by hydrogenolysis, e.g. with hydrogen in the presence of a catalyst such as palladium/charcoal in a solvent such as methanol, ethanol, ethyl acetate or glacial acetic acid, optionally with the addition of an acid such as hydrochloric acid at temperatures between 0.degree. and 50.degree. C., but preferably at ambient temperature, under a hydrogen pressure of 1 to 7 bar, preferably of 3 to 5 bar.
An isomer mixture of a compound of general formula I thus obtained may if desired be resolved by chromatography using a substrate such as silica gel or aluminium oxide.
Moreover, the compounds of general formula I obtained may be converted into the acid addition salts thereof, more particularly for pharmaceutical use the physiologically acceptable salts thereof with inorganic or organic acids. Suitable acids for this purpose include hydrochloric acid, hydrobromic acid, sulphuric acid, phosphoric acid, fumaric acid, succinic acid, lactic acid, citric acid, tartaric acid or maleic acid.
Furthermore, the new compounds of general formula I thus obtained, if they contain a carboxy or 1H-tetrazolyl group, may if desired subsequently be converted into the addition salts thereof with inorganic or organic bases, more particularly for pharmaceutical use into the physiologically acceptable addition salts thereof. Suitable bases include for example sodium hydroxide, potassium hydroxide, cyclohexylamine, ethanolamine, diethanolamine and triethanolamine.
The compounds of general formulae II to XVI used as starting materials are known from the literature in some cases or may be obtained by methods known from the literature.
Thus, for example, substituted pyrimidines of general formula II are described in Ep-A-0424317, substituted pyrimidinones are described in Ep-A-0407342 and Ep-A-0419048, substituted triazoles are described in Ep-A-0409332, substituted triazolinones, triazolthiones and triazolimines are described in Ep-A-0412594, substituted pyrazoles in Ep-A-0411507, substituted quinazolinones in Ep-A-0411766, substituted quinolines in Ep-A-0412848, 5-membered condensed imidazo derivatives in Ep-A-0407102, benzimidazoles in Ep-A-0392317, imidazopyridines and purines in Ep-A-0400974 and Ep-A-0399731 and substituted imidazoles in Ep-A-0253310.
The compounds of general formulae III and IV used as starting materials are obtained by reacting a corresponding alcohol with hydrohalic acid, with tetrabromomethane/triphenylphosphine or with a corresponding sulphonic acid halide.
The compounds of general formulae VIII, IX, X, XII, XIV and XVI used as starting materials are obtained by alkylating a corresponding compound with a compound analogous to a compound of general formula III.
The new compounds of general formula I and the physiologically acceptable addition salts thereof have valuable pharmacological properties. They are angiotensin antagonists, particularly angiotensin-II-antagonists.
For example, the compounds
A=2-n-propyl-4-methyl-1-[4-[(.alpha.-carboxy)benzyloxy]-benzyl]benzimidazole,
B=2-n-butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-dimethylaminocarbonylamino-benzimidazole,
C=2-n-propyl-6-(1-methyl-benzimidazol-2-yl)-4-methyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole,
D=2-methyl-4-[4'-[(.alpha.-carboxy)benzyloxy]benzyloxy]-quinoline,
E=2-n-butyl-8-methyl-3-[4-[(.alpha.-carboxy)benzyloxy]-benzyl]quinazolin-4-one-semihydrate,
F=2-n-propyl-4-methyl-6-(1-methyl-benzimidazol-2-yl)-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]-benzimidazole,
G=2-n-butyl-6-(N-propionyl-methylamino)-1-[4-[(1-carboxy-3-methyl) butyloxy]benzyl]benzimidazole,
H=2-n-butyl-5-methyl-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinon-1-yl)-3-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]imidazol[4,5-b]pyridine and
I=2-n-butyl-1-[4-[[.alpha.-[.alpha.-ethyl-phosphono]benzylamino]benzyl]benzimidazole
were investigated for their biological effects as follows:
Description of method: Angiotensin II-receptor bonding
The tissue (rats lung) is homogenised in Tris-buffer (50 mMol Tris, 150 mMol NaCl, 5 mMol EDTA, pH 7.40) and centrifuged twice for 20 minutes at 20,000x g. The finished pellets are resuspended in incubating buffer (50 mMol Tris, 5 mMol MgCl.sub.2, 0.2% BSA, pH 7.40) 1:75, based on the moist weight of the tissue. Each 0.1 ml of homogenate is incubated for 60 minutes at 37.degree. C. with 50 pM [.sup.125 I]-angiotensin II (NEN, Dreieich, FRG) with increasing concentrations of the test substance in a total volume of 0.25 ml. Incubation is ended by rapid filtration through glass fibre filter mats. The filters are each washed with 4 ml of ice cold buffer (25 mMol Tris, 2.5 mMol MgCl.sub.2, 0.1% BSA, pH 7.40). The bound radioactivity is measured using a gamma-counter. The corresponding IC.sub.50 value is obtained from the dose-activity curve.
In the test described, substances A to I show the following IC.sub.50 values:
______________________________________ Substance IC.sub.50 [nM]______________________________________ A 76 B 8 C 4 D 2000 E 2000 F 12 G 830 H 27 I 560______________________________________
Moreover, when the above-mentioned compounds were administered in a dose of 30 mg/kg i.v. no toxic side effects, e.g. negative inotropic effects or disorders in heart rhythm, were observed. The compounds are therefore well tolerated.
In view of their pharmacological properties, the new compounds and the physiologically acceptable addition salts thereof are suitable for the treatment of hypertension and cardiac insufficiency and also for treating ischaemic peripheral circulatory disorders, myocardial ischaemia (angina), for the prevention of the progression of cardiac insufficiency after myocardial infarction and for treating diabetic nephropathy, glaucoma, gastrointestinal diseases and bladder diseases.
The new compounds and the physiologically acceptable addition salts thereof are also suitable for treating pulmonary diseases, e.g. lung oedema and chronic bronchitis, for preventing arterial re-stenosis after angioplasty, for preventing thickening of blood vessel walls after vascular operations, and for preventing arteriosclerosis and diabetic angiopathy. In view of the effects of angiotensin on the release of acetylcholine and dopamine in the brain, the new angiotensin antagonists are also suitable for alleviating central nervous system disorders, e.g. depression, Alzheimer's disease, parkinson syndrome, bulimia and disorders of cognitive function.
The dosage required to achieve these effects in adults is appropriately, when administered intravenously, 20 to 100 mg, preferably 30 to 70 mg, and, when administered orally, 50 to 200 mg, preferably 75 to 150 mg, 1 to 3 times a day. For this purpose, the compounds of general formula I prepared according to the invention, optionally in conjunction with other active substances, such as hypotensives, diuretics and/or calcium antagonists, may be incorporated together with one or more inert conventional carriers and/or diluents, e.g. with corn starch, lactose, glucose, microcrystalline cellulose, magnesium stearate, polyvinylpyrrolidone, citric acid, tartaric acid, water, water/ethanol, water/glycerol, water/sorbitol, water/polyethylene-glycol, propylene-glycol, cetylstearyl alcohol, carboxymethylcellulose or fatty substances such as hard fat or suitable mixtures thereof, in conventional galenic preparations such as plain or coated tablets, capsules, powders, suspensions or suppositories.
Additional active substances which may be included in the combinations mentioned above might be, for example, bendroflumethiazide, chlorothiazide, hydrochlorothiazide, spironolactone, benzothiazide, cyclothiazide, ethacrinic acid, furosemide, metoprolol, prazosine, atenolol, propranolol, (di)hydralazine-hydrochloride, diltiazem, felodipin, nicardipin, nifedipin, nisoldipin and nitrendipin. The dosage for these active substances is appropriately one fifth of the lowest recommended dose up to 1/1 of the normally recommended dose, i.e., for example, 15 to 200 mg of hydrochlorothiazide, 125 to 2000 mg of chlorothiazide, 15 to 200 mg of ethacrinic acid, 5 to 80 mg of furosemide, 20 to 480 mg of propranolol, 5 to 60 mg of felodipine, 5 to 60 mg of nifedipin or 5 to 60 mg of nitrendipin.
The Examples which follow are intended to illustrate the invention:

EXAMPLE A
4-(.alpha.-Ethoxycarbonyl-benzyloxy)benzylbromide
a) Ethyl 2-bromo-phenylacetate
43.0 g (0.2 mol) of DL-2-bromo-phenylacetic acid are dissolved in 400 ml of ethanol at ambient temperature with stirring. At 5.degree.-10.degree. C. 11.8 g (0.2 mol) of thionylchloride are slowly added dropwise whilst cooling with ice. After 12 hours at ambient temperature the solvent is distilled off and the residue is taken up in ethyl acetate. After extraction with saturated sodium bicarbonate solution and saturated saline solution the product is dried over sodium sulphate and evaporated down.
Yield: 41.85 g (86% of theory), R.sub.f value: 0.60 (silica gel; eluant: ethyl acetate/petroleum ether=1:19)
b) 4-(.alpha.-Ethoxycarbonyl-benzyloxy)benzyl alcohol
41.8 g (0.172 mol) of ethyl 2-bromo-phenylacetate and 21.3 g (0.172 mol) of 4-hydroxybenzyl alcohol are dissolved in 850 ml of acetone and 24.8 g (0.18 mol) of potassium carbonate and 5.0 g (0.03 mol) of potassium iodide are added thereto. The reaction mixture is refluxed for 60 hours with stirring. Then the inorganic salts are filtered off and the residue is washed with hot acetone. The filtrate is evaporated down and the residue is purified over a silica gel column (particle size: 0.063-0.02 mm), initially using petroleum ether as eluant and then using mixtures of petroleum ether and ethyl acetate of increasing polarity (9:1, 8:2 and 7:3). The unified fractions are evaporated down in vacuo.
Yield: 30.65 g (62.5% of theory), R.sub.f value: 0.40 (silica gel; eluant: ethyl acetate/petroleum ether=3:7)
c) 4(.alpha.-Ethoxycarbonyl-benzyloxy)benzylbromide
28.6 g (0.1 mol) of 4-(.alpha.-ethoxycarbonyl-benzyloxy)benzyl alcohol are dissolved in 300 ml of dichloromethane and mixed with 31.6 g (0.12 mol) of triphenylphosphine. Whilst cooling with ice, a solution of 40.0 g (0.12 mol) of carbon tetrabromide in 100 ml of dichloromethane is added dropwise. The mixture is stirred for 30 minutes at ambient temperature and then concentrated by evaporation. The residue is purified over a silica gel column (particle size: 0.063-0.02 mm), using as eluant first petroleum ether and then mixtures of petroleum ether and ethyl acetate of increasing polarity (9:1 and 8:2). The unified fractions are evaporated down in vacuo.
Yield: 20.45 g (59% of theory), Oil, R.sub.f value: 0.70 (silica gel; eluant: ethyl acetate/petroleum ether=1:4) C.sub.17 H.sub.16 BrO.sub.3 (349.24) Calculated: C 58.20 H 4.95 Br 22.80 Found: 58.23 4.84 23.12
EXAMPLE 1
2-n-Propyl-4-methyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
2-n-Propyl-4-methyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole
350 mg (2.0 mMol) of 2-n-propyl-4-methyl-benzimidazole (prepared analogously to Chemistry of Heterocyclic Compounds, Vol. 40, part 1, 6-12) are added to a solution of 220 mg of potassium tert.butoxide in 30 ml of dimethylsulphoxide. After 30 minutes at ambient temperature a solution of 700 mg (2.0 mMol) of 4-(.alpha.-ethoxycarbonyl-benzyloxy)benzylbromide in 5 ml of dimethylsulphoxide is added dropwise. After 12 hours at ambient temperature the reaction solution is stirred into ice water and extracted 3 times with 100 ml of ethyl acetate. The combined organic phases are washed with 100 ml of saline solution and dried over sodium sulphate. After the solvent has been eliminated, the residue is purified over a silica gel column (particle size 0.063-0.02 mm), using as eluant methylene chloride to start with, then mixtures of methylene chloride and ethanol of increasing polarity (50:1 and 25:1). The uniform fractions are concentrated by evaporation in vacuo.
Yield: 500 mg (55% of theory), Oil, R.sub.f value: 0.55 (silica gel; eluant: methylene chloride/methanol=19:1)
b) 2-n-Propyl-4-methyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
500 mg (1.1 mMol) of 2-n-propyl-4-methyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole are dissolved in 10 ml of ethanol and mixed with 10 ml of 1N sodium hydroxide solution. The mixture is stirred at ambient temperature for 30 minutes. Then the mixture is concentrated by evaporation and taken up in water. The solution is acidified by the addition of glacial acetic acid. The precipitate formed is suction filtered, washed with water until neutral and dried. The crude product is taken up in methylene chloride and purified over a silica gel column (particle size 0.063-0.02 mm), using as eluant a mixture of methylene chloride and methanol (19: 1). The uniform fractions are concentrated by evaporation in vacuo and the residue is triturated with hexane/ether and suction filtered.
Yield: 60 mg (13% of theory), Melting point: amorphous C.sub.26 H.sub.26 N.sub.2 O.sub.3 (414.51) Mass spectrum: (M+H).sup.+ =415
EXAMPLE 2
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-propionylamino-benzimidazole-hydrochloride
a) 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-nitro-benzimidazole and 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-5-nitro-benzimidazole
Prepared analogously to Example la from 2-n-butyl-5-nitrobenzimidazole (prepared analogously to Chemistry of Heterocyclic Compounds, Vol. 40, Part 1, 6-12) and 4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzylbromide.
Yield: 70.5% of theory, Oil, R.sub.f value: 0.25 (silica gel; eluant: methylene chloride/ethanol=50:1)
b) 2-n-Butyl-6-amino-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-benzyl]-benzimidazole
Prepared by catalytic hydrogenation of 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-5/6-nitro-benzimidazole in the presence of Raney nickel in ethanol at 50.degree. C. under 5 bars of hydrogen pressure, followed by chromatographic separation of the 5-amino-2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)-benzyloxy]benzyl]benzimidazole.
Yield: 21.8% of theory, Oil, R.sub.f value: 0.30 (silica gel; eluant: petroleum ether/methylene chloride/ethanol=7:2:1)
2-n-Butyl-6-propionylamino-1-[4-[(.alpha.-ethoxycarbonyl)-benzyloxy]benzyl]benzimidazole
Prepared from 2-n-butyl-6-amino-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole and propionyl chloride/pyridine.
Yield: 70.2% of theory, Melting point: 207.degree.-209.degree. C.
d) 2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-propionylamino-benzimidazole-hydrochloride
Prepared analogously to Example 1b from 2-n-butyl-6-propionylamino-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 43.5% of theory, Melting point: sinters from 93.degree. C. C.sub.29 H.sub.31 N.sub.3 O.sub.4 .times.HCl (522.67) Calculated: C 66.50 H 6.12 N 8.08 Found: 66.73 6.34 7.81
EXAMPLE 3
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-dimethylaminocarbonylamino-benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-dimethylamino-carbonylaminobenzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 80% of theory, Melting point: 183.degree.-185.degree. C. C.sub.29 H.sub.32 N.sub.4 O.sub.4 (500.60) Calculated: C 69.59 H 6.44 N 11.19 Found: 69.54 6.43 10.66
EXAMPLE 4
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-cyclohexylaminocarbonylamino-benzimidazole-hydrochloride
a) 2-n-Butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-cyclohexylaminocarbonylamino-benzimidazole
Prepared from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-amino-benzimidazole and cyclohexylisocyanate/triethylamine in tetrahydrofuran.
Yield: 65.4% of theory, Oil, R.sub.f value: 0.40 (silica gel; eluant: methylene chloride/ethanol=19:1)
b) 2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-cyclohexylaminocarbonylamino-benzimidazole-hydrochloride
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-cyclohexylamino-carbonylaminobenzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 40.7% of theory, Melting point: sinters from 184.degree. C. C.sub.33 H.sub.38 N.sub.4 O.sub.4 .times.HCl (591.17) Calculated: C 67.00 H 6.60 N 9.45 Found: 67.15 6.71 9.30
EXAMPLE 5
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-5-cyclohexylaminocarbonylamino-benzimidazole-hydrochloride
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-5-cyclohexylamino-carbonylaminobenzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 46.1% of theory, Melting point: sinters from 196.degree. C. C.sub.33 H.sub.38 N.sub.4 O.sub.4 .times.HCl (591.17) Calculated: C 67.00 H 6.60N 9.45 Found: 67.22 6.74 9.49
EXAMPLE 6
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-(3,4,5,6-tetrahydro-2(1H)-pyrimidinon-1-yl)-benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-(3,4,5,6-tetrahydro-2(1H)-pyrimidinon-1-yl)-benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 8.8% of theory, Melting point: 260.degree.-262.degree. C. C.sub.30 H.sub.32 N.sub.4 O.sub.4 (512.61) Mass spectrum: (M+H).sup.+ =513
EXAMPLE 7
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinon-1-yl)-benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinon-1-yl)-benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 80.4% of theory, Melting point: 124.degree.-126.degree. C. C.sub.37 H.sub.38 N.sub.4 O.sub.4 (602.74) Calculated: C 73.73 H 6.35N 9.30 Found: 73.47 6.50 9.05
EXAMPLE 8
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-(N-cyclohexylaminocarbonyl-methylamino)-benzimidazole
a) 2-n-Butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-(N-cyclohexylaminocarbonyl-methylamino)-benzimidazole
Prepared analogously to Example 1a from 2-n-butyl-6-(N-cyclohexylaminocarbonyl-methylamino)benzimidazole and 4-[(.alpha.-ethoxycarbonyl)-benzyloxy]benzylbromide and subsequent chromatographic separation of the 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-5-(N-cyclohexylaminocarbonyl-methylamino)-benzimidazole.
Yield: 50.0% of theory, Oil, R.sub.f value: 0.50 (silica gel; eluant: methylethyl ketone/xylene=1:1)
b) 2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-(N-cyclohexylaminocarbonyl-methylamino)benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-(N-cyclohexyl-aminocarbonylmethylamino)benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 93.0% of theory, Melting point: 168.degree.-169.degree. C. C.sub.34 H.sub.40 N.sub.4 O.sub.4 (568.72) Calculated: C 71.81 H 7.09N 9.85 Found: 71.78 7.02 9.71
EXAMPLE 9
2-n-propyl-5-(2-methyl-propionylamino)-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazo[4,5-b]pyridine
a) 2-n-Propyl-5-(2-methyl-propionylamino)-imidazo[4,5-b]pyridine
2.62 g (15 mMol) of 2-n-propyl-5-amino-imidazo[4,5-b]pyridine are suspended in 100 ml of absolute methylene chloride and 3.16 g (30 mMol) of isobutyric acid chloride are added, with stirring and cooling with ice. Then, at -5.degree. C., a solution of 3.03 g (30 mMol) of triethylamine in 5 ml of methylene chloride is added dropwise. After one hour at ambient temperature the reaction mixture is combined with 100 ml of 2N hydrochloric acid and the mixture is stirred for a further hour. Then the solution is poured onto ice water and mixed with saturated sodium hydrogen carbonate solution. The aqueous phase is extracted 3 times with 100 ml of ethyl acetate, the combined organic phases are then washed with saline solution, dried over sodium sulphate and concentrated by evaporation. The crude product is taken up in methylene chloride and purified over a silica gel column (particle size 0.063-0.2 mm), using as eluant methylene chloride to begin with, followed by mixtures of methylene chloride and ethanol of increasing polarity (25:1 and 19:1). The unified fractions are concentrated by evaporation and the residue is triturated with petroleum ether/ether=1:1 and suction filtered.
Yield: 2.05 g (56% of theory), Melting point: 206.degree. C. C.sub.13 H.sub.18 N.sub.4 O (246.30) Calculated: C 63.39 H 7.37N 22.75 Found: 63.64 7.57 22.98
b) 2-n-Propyl-5-(2-methyl-propionylamino)-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]imidazo[4,5-b]-pyridine
Prepared analogously to Example 1a from 2-n-propyl-5-(2-methylpropionylamino)imidazo[4,5-b]pyridine and 4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzylbromide.
Yield: 33.7% of theory, Melting point: 100.degree.-101.degree. C.
c) 2-n-Propyl-5-(2-methyl-propionylamino)-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 1b from 2-n-propyl-5-(2-methylpropionylamino)-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]imidazo[4,5-b]pyridine and 1N sodium hydroxide solution in ethanol.
Yield: 52.0% of theory, Melting point: 134.degree. C. C.sub.28 H.sub.30 N.sub.4 O.sub.4 (486.58) Calculated: C 69.12 H 6.21N 11.51 Found: 69.03 6.11 11.36
EXAMPLE 10
2-n-Butyl-5-valeroylamino-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazo[4,5-b]pyridine-semihydrate
Prepared analogously to Example 1b from 2-n-butyl-5-valeroylamino-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]imidazo[4,5-b]pyridine and 1N sodium hydroxide solution in ethanol.
Yield: 57.0% of theory, Melting point: sinters from 96.degree. C. C.sub.30 H.sub.34 N.sub.4 O.sub.4 .times.0.5 H.sub.2 O (532.65) Calculated: C 68.82 H 6.74 N 10.70 Found: 68.99 6.67 10.58
EXAMPLE 11
2-n-Propyl-5-(1-methyl-benzimidazol-2-yl)-7-methyl-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 1b from 2-n-propyl-5-(1-methyl-benzimidazol-2-yl)-7-methyl-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl-imidazo[4,5-b]pyridine and 1N sodium hydroxide solution in ethanol.
Yield: 41.4% of theory, Melting point: 242.degree.-244.degree. C. C.sub.33 H.sub.31 N.sub.5 O.sub.3 (545.65) Calculated: C 72.64 H 5.73 N 12.83 Found: 72.51 5.75 12.97
EXAMPLE 12
2-n-Propyl-6-(1-methyl-benzimidazol-2-yl)-4-methyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-propyl-6-(1-methyl-benzimidazol-2-yl)-4-methyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 41.0% of theory, Melting point: amorphous C.sub.34 H.sub.32 N.sub.4 O.sub.3 (544.66) Calculated: C 74.98 H 5.92 N 10.28 Found: 74.55 6.06 10.08 Mass spectrum: (M+H).sup.+ =545
EXAMPLE 13
2-Methyl-4-[4'-[(.alpha.-carboxy)benzyloxy]benzyloxy]quinoline
a) 2-Methyl-4-[4'-[(.alpha.-ethoxycarbonyl)benzyloxy]-benzyloxy]quinoline
Prepared analogously to Example 1a from 4-hydroxy-quinaldine and 4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl-bromide.
Yield: 19.8% of theory, Oil, R.sub.f value: 0.55 (silica gel; eluant: methylene chloride/ethanol=19:1)
b) 2-Methyl-4-[4'-[(.alpha.-carboxy)benzyloxy]benzyloxy]-quinoline
Prepared analogously to Example 1b from 2-methyl-4-[4'-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyloxy-quinoline and 1N sodium hydroxide solution in ethanol.
Yield: 66.6% of theory, Melting point: 142.degree.-143.degree. C. C.sub.25 H.sub.21 NO.sub.4 (399.50) Calculated: C 73.17 H 5.30 N 3.51 Found: 73.56 5.25 3.34 Mass spectrum: (M+H).sup.+ =400
EXAMPLE 14
2-n-Propyl-5-n-butyrylamino-3-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]imidazo[4,5-b]pyridine
a) 2-n-Propyl-5-n-butyrylamino-3-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 1a from 2-n-Propyl-5-n-butyrylamino-imidazo[4,5-b]pyridine and 4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzylbromide, with subsequent chromatographic separation of the 2-n-propyl-5-n-butyrylamino-1-[4-[(1-methoxycarbonyl-3-methyl)-butyloxy]benzyl]imidazo[4,5-b]pyridine.
Yield: 47.0% of theory, Oil, R.sub.f value: 0.30 (silica gel; eluant: methylene chloride/ethanol=19:1)
b) 2-n-Propyl-5-n-butyrylamino-3-[4-(1-carboxy-3-methyl)-butyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 1b from 2-n-propyl-5-n-butyrylamino-3-[4-(1-methoxycarbonyl-3-methyl)-butyloxy]benzyl]imidazo[4,5-b]pyridine and 1N sodium hydroxide solution in ethanol.
Yield: 59.0% of theory, Melting point: 147.degree.-148.degree. C. C.sub.26 H.sub.34 N.sub.4 O.sub.4 (466.59) Calculated: C 66.93 H 7.35 N 12.01 Found: 66.69 7.48 11.85
EXAMPLE 15
2-n-Butyl-1-[4-[(.alpha.-carboxy)cyclohexylmethyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-methoxycarbonyl)cyclohexylmethyloxy]benzyl]-benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 92.0% of theory, Melting point: 208.degree. C. (decomp.) C.sub.26 H.sub.32 N.sub.2 O.sub.3 (420.56) Calculated: C 74.25 H 7.66 N 6.66 Found: 73.98 7.52 6.41
EXAMPLE 16
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazole
a) 2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-methoxycarbonyl)benzyloxy]benzyl]imidazole
Prepared analogously to Example 1a from 2-n-butyl-4-chloro-5-hydroxymethyl-imidazole and 4-[(.alpha.-methoxycarbonyl]benzyloxy]benzylbromide, with subsequent chromatographic separation of the 2-n-butyl-5-chloro-4-hydroxymethyl-1-[4-[(.alpha.-methoxycarbonyl)benzyloxy]benzyl]imidazole.
Yield: 21.0% of theory, Oil, R.sub.f value: 0.70 (silica gel; eluant: methylene chloride/methanol=6:1)
b) 2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-carboxy)-benzyloxy]benzyl]imidazole-hydrochloride
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-methoxycarbonyl-benzyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 34.0% of theory, Melting point: 176.degree. C. C.sub.23 H.sub.25 ClN.sub.2 O.sub.4 HCl (465.38) Calculated: C 59.36 H 5.63 6.02 Found: 59.53 5.43 5.97
EXAMPLE 17
2-n-Butyl-5-chloro-4-hydroxymethyl-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-5-chloro-4-hydroxymethyl-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 66.0% of theory, Melting point: 175.degree.-176.degree. C. C.sub.21 H.sub.29 ClN.sub.2 O.sub.4 (408.93) Calculated: C 61.68 H 7.14 N 6.84 Found: 61.34 7.11 6.58
EXAMPLE 18
2-n-Butyl-5-chloro-4-hydroxymethyl-1-[4-[(1-carboxy)-n-propyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-5-chloro-4-hydroxymethyl-1-[4-[(1-methoxycarbonyl)-n-propyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 70.0% of theory, Melting point: 182.degree.-184.degree. C. C.sub.19 H.sub.25 ClN.sub.2 O.sub.4 (380.88) Calculated: C 59.91 H 6.61 N 7.35 Found: 59.67 6.53 7.22
EXAMPLE 19
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(1-carboxy)-n-propyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxymethyl-1-[4-[(1-methoxycarbonyl)-n-propyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 66.0% of theory, Melting point: 125.degree.-127.degree. C. C.sub.19 H.sub.25 ClN.sub.2 O.sub.4 (380.88) Calculated: C 59.91 H 6.61 N 7.35 Found: 59.74 6.60 6.90
EXAMPLE 20
2-n-Butyl-4-chloro-5-methoxymethyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-methoxymethyl-1-[4-[(.alpha.-methoxycarbonyl)-benzyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 45.0% of theory, Melting point: 156.degree.-158.degree. C. C.sub.24 H.sub.27 ClN.sub.2 O.sub.4 (442.95) Calculated: C 65.08 H 6.14 N 6.32 Found: 64.70 6.38 6.03
EXAMPLE 21
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxy-methyl-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 57.0% of theory, Melting point: 164.degree.-165.degree. C. C.sub.21 H.sub.29 ClN.sub.2 O.sub.4 (408.93) Calculated: C 61.68 H 7.14 N 6.84 Found: 61.63 7.09 6.71
EXAMPLE 22
2-n-Butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-methoxycarbonyl)benzyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 18.0% of theory, Melting point: 193.degree. C. C.sub.23 H.sub.25 ClN.sub.2 O.sub.4 (428.92) R.sub.f value: 0.10 (silica gel; eluant: methylene chloride/methanol=9:1) Mass spectrum: (M+H).sup.+ =394/396 (Cl)
EXAMPLE 23
2-n-Butyl-5-chloro-4-hydroxymethyl-1-[4-[(1-carboxy)-n-butyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-5-chloro-4-hydroxymethyl-1-[4-[(1-methoxycarbonyl)-n-butyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 27.0% of theory, Melting point: 154.degree. C. C.sub.20 H.sub.27 ClN.sub.2 O.sub.4 (394.90) R.sub.f value: 0.20-0.30 (silica gel; eluant: methylene chloride/methanol=9:1) Mass spectrum: (M+H).sup.+ =394/396 (Cl)
EXAMPLE 24
2-n-Butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-carboxy)-p-phenylbenzyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-methoxycarbonyl)-p-phenylbenzyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 72.0% of theory, Melting point: 182.degree. C. C.sub.29 H.sub.29 ClN.sub.2 O.sub.4 (505.02) Calculated: C 68.97 H 5.78 N 5.54 Found: 68.50 5.83 5.46
EXAMPLE 25
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-carboxy)-p-phenylbenzyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-methoxycarbonyl)-p-phenylbenzyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 75.0% of theory, Melting point: 185.degree.-186.degree. C. (decomp.) C.sub.29 H.sub.29 ClN.sub.2 O.sub.4 (505.02) Calculated: C 68.97 H 5.78 N 5.54 Found: 68.87 5.80 5.42
EXAMPLE 26
2-n-Butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-carboxy)-2-naphthylmethyloxy]benzyl]imidazole-semihydrate
Prepared analogously to Example 1b from 2-n-butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-methoxycarbonyl)-2-naphthylmethyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 69.0% of theory, Melting point: from 188.degree. C. (decomp.) C.sub.27 H.sub.27 ClN.sub.2 O.sub.4 .times.0.5 H.sub.2 O (487.99) Calculated: C 66.45 H 5.78 N 5.74 Found: 66.63 5.66 5.75
EXAMPLE 27
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-carboxy)-2-naphthylmethyloxy]benzyl]imidazole-semihydrate
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-methoxycarbonyl)-2-naphthylmethyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 82.0% of theory, Melting point: from 142.degree. C. (decomp.) C.sub.27 H.sub.27 ClN.sub.2 O.sub.4 .times.0.5 H.sub.2 O (487.99) Calculated: C 66.45 H 5.78 N 5.74 Found: 66.62 5.66 6.12
EXAMPLE 28
2-n-Butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-carboxy)-1-naphthylmethyloxy]benzyl]imidazole-hydrate
Prepared analogously to Example 1b from 2-n-butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-methoxycarbonyl)-1-naphthylmethyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 91.0% of theory, Melting point: from 110.degree. C. (decomp.) C.sub.27 H.sub.27 ClN.sub.2 O.sub.4 .times.H.sub.2 O (497.00) Calculated: C 65.25 H 5.88 N 5.63 Found: 65.58 5.89 5.83
EXAMPLE 29
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-carboxy)-1-naphthylmethyloxy]benzyl]imidazole-hydrate
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-methoxycarbonyl)-1-naphthylmethyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 42.0% of theory, Melting point: from 100.degree. C. (decomp.) C.sub.27 H.sub.27 ClN.sub.2 O.sub.4 .times.H.sub.2 O (497.00) Calculated: C 65.25 H 5.88 N 5.63 Found: 65.21 5.70 6.10
EXAMPLE 30
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-carboxy)-p-methoxybenzyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxymethyl-1-[4-[(.alpha.-methoxycarbonyl)-p-methoxybenzyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 58.0% of theory, Melting point: 165.degree. C. C.sub.24 H.sub.27 ClN.sub.2 O.sub.5 (458.95) Calculated: C 62.81 H 5.93 N 6.10 Found: 62.69 6.02 5.93
EXAMPLE 31
2-n-Butyl-4-chloro-5-methoxymethyl-1-[4-[(1-carboxy)-n-pentyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-methoxymethyl-1-[4-[(1-methoxycarbonyl)-n-pentyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 84.0% of theory, Melting point: 168.degree. C. C.sub.22 H.sub.31 ClN.sub.2 O.sub.4 (422.96) Calculated: C 62.45 H 7.38 N 6.62 Found: 62.23 7.47 6.79
EXAMPLE 32
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(1-carboxy)-n-butyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxymethyl-1-[4-[(1-methoxycarbonyl)-n-butyloxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 50.0% of theory, Melting point: 137.degree.-138.degree. C. C.sub.20 H.sub.27 ClN.sub.2 O.sub.4 (394.90) Calculated: C 60.83 H 6.89 N 7.09 Found: 60.67 6.94 6.67
EXAMPLE 33
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(1-carboxy)-n-pentyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxymethyl-1-[4-[(1-methoxycarbonyl)-n-pentyloxy]benzyl]imidazole and 1N sodium hydroxide solution ethanol.
Yield: 61.0% of theory, Melting point: 150.degree. C. C.sub.21 H.sub.29 ClN.sub.2 O.sub.4 (408.93) Calculated: C 61.65 H 7.14 N 6.85 Found: 62.31 6.85 7.21
EXAMPLE 34
2-n-Butyl-4-hydroxymethyl-5-chloro-1-[4-[(1-carboxy)-n-pentyloxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-hydroxymethyl-5-chloro-1-[4-[(1-methoxycarbonyl)-n-pentyloxy]benzyl]imidazole and 1N sodium hydroxide solution ethanol.
Yield: 57.0% of theory, Melting point: 168.degree. C. C.sub.21 H.sub.29 ClN.sub.2 O.sub.4 (408.93) Calculated: C 61.65 H 7.14 N 6.85 Found: 61.59 7.11 6.91
EXAMPLE 35
2-n-Butyl-8-methyl-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-quinazolin-4-one-semihydrate
a) 2-n-Butyl-8-methyl-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]quinazolin-4-one
Prepared analogously to Example 1a from 2-n-butyl-8-methyl-quinazolin-4(1H)-one and 4-(.alpha.-ethoxycarbonylbenzyloxy)benzylbromide.
Yield: 37% of theory, Oil, R.sub.f value: 0.45 (silica gel; eluant: petroleum ether/ethyl acetate=4:1)
b) 2-n-Butyl-8-methyl-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]quinazolin-4-one-semihydrate
Prepared analogously to Example 1b from 2-n-butyl-8-methyl-3-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-quinazolin-4-one and 1N sodium hydroxide solution in ethanol.
Yield: 84% of theory, Melting point: 201.degree.-204.degree. C. C.sub.28 H.sub.28 N.sub.2 O.sub.4 .times.0.5 H.sub.2 O (465.56) Calculated: C 72.24 H 6.28 N 6.02 Found: 72.41 6.33 5.75
EXAMPLE 36
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[4-[(1-carboxy-2-cyclohexyl)ethoxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-chloro-5-hydroxymethyl-1-[4-[(1-methoxycarbonyl-2-cyclohexyl)ethoxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 67.1% of theory, Melting point: 145.degree.-150.degree. C. C.sub.24 H.sub.33 ClN.sub.2 O.sub.4 (448.96) Calculated: C 64.20 H 7.40 N 6.24 Found: 63.97 7.38 6.01
EXAMPLE 37
2-n-Butyl-4-hydroxymethyl-5-chloro-1-[4-[(1-carboxy-2-cyclohexyl)ethoxy]benzyl]imidazole
Prepared analogously to Example 1b from 2-n-butyl-4-hydroxymethyl-5-chloro-1-[4-[(1-methoxycarbonyl-2-cyclohexyl)ethoxy]benzyl]imidazole and 1N sodium hydroxide solution in ethanol.
Yield: 59.1% of theory, Melting point: 180.degree.-183.degree. C. C.sub.24 H.sub.33 ClN.sub.2 O.sub.4 (448.96) Calculated: C 64.20 H 7.40 N 6.24 Found: 63.99 7.20 6.27 Mass spectrum: m/e=448/450 (Cl)
EXAMPLE 38
2-n-Butyl-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]-benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 82.3% of theory, Melting point: 150.degree.-152.degree. C. C.sub.24 H.sub.30 N.sub.2 O.sub.3 (394.52) Calculated: C 73.07 H 7.66 N 7.10 Found: 72.83 7.37 7.14 Mass spectrum: m/e=394
EXAMPLE 39
2-n-Butyl-1-[4-[(1-carboxy-2-cyclohexyl)ethoxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(1-methoxycarbonyl-2-cyclohexyl)ethoxy]benzyl]-benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 82.0% of theory, Melting point: 165.degree.-170.degree. C. C.sub.27 H.sub.34 N.sub.2 O.sub.3 (434.58) Calculated: C 74.63 H 7.88 N 6.44 Found: 74.46 7.77 6.30 Mass spectrum: m/e=434
EXAMPLE 40
2-n-Butyl-6-propionylamino-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-6-propionylamino-1-[4-[(1 -methoxycarbonyl-3-methyl)butyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 80.3% of theory, Melting point: 120.degree.-130.degree. C. C.sub.27 H.sub.35 N.sub.3 O.sub.4 (465.59) Calculated: C 69.65 H 7.58 N 9.02 Found: 69.30 7.84 8.83 Mass spectrum: m/e=465
EXAMPLE 41
2-n-Butyl-5-propionylamino-1-[4-[(1-carboxy-3-methyl)-butyloxy]benzyl]benzimidazole-semihydrate
Prepared analogously to Example 1b from 2-n-butyl-5-propionylamino-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 82.9% of theory, Melting point: 165.degree.-170.degree. C. C.sub.27 H.sub.35 N.sub.3 O.sub.4 .times.0.5 H.sub.2 O (474.60) Calculated: C 68.32 H 7.64 N 8.85 Found: 68.63 7.72 8.90
EXAMPLE 42
2-n-Butyl-6-cyclohexylaminocarbonylamino-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-6-cyclohexylaminocarbonylamino-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 90.8% of theory, Melting point: 155.degree.-160.degree. C. C.sub.31 H.sub.42 N.sub.4 O.sub.4 (534.71) Calculated: C 69.64 H 7.92 N 10.48 Found: 69.58 8.03 10.36 Mass spectrum: (M+H).sup.+ =535
EXAMPLE 43
2-n-Butyl-5-cyclohexylaminocarbonylamino-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-5-cyclohexylaminocarbonylamino-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 95.7% of theory, Melting point: 220.degree.-225.degree. C. C.sub.31 H.sub.42 N.sub.4 O.sub.4 (534.71) Calculated: C 69.64 H 7.92 N 10.48 Found: 69.49 7.99 10.52 Mass spectrum: (M+H).sup.+ =535
EXAMPLE 44
2-n-Butyl-6-(N-propionyl-methylamino)-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-6-(N-propionylmethylamino)-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 66.8% of theory, Melting point: 170.degree.-175.degree. C. C.sub.28 H.sub.37 N.sub.3 O.sub.4 (479.63) Calculated: C 70.12 H 7.78 N 8.76 Found: 70.35 7.85 8.91
EXAMPLE 45
2-n-Butyl-5-(N-propionyl-methylamino)-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-5-(N-propionylmethylamino)-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 62.0% of theory, Melting point: 172.degree.-175.degree. C. C.sub.28 H.sub.37 N.sub.3 O.sub.4 (479.63) Calculated: C 70.12 H 7.78 N 8.76 Found: 70.11 7.80 8.63 Mass spectrum: m/e=479
EXAMPLE 46
2-n-Butyl-6-(N-cyclohexylaminocarbonyl-methylamino)-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-6-(N-cyclohexylaminocarbonyl-methylamino)-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 76.1% of theory, Melting point: 170.degree.-175.degree. C. C.sub.32 H.sub.44 N.sub.4 O.sub.4 (548.73) Calculated: C 70.04 H 8.08 N 10.21 Found: 69.95 8.10 10.22
EXAMPLE 47
2-n-Butyl-5-(N-cyclohexylaminocarbonyl-methylamino)-1-[4-[(1-carboxy-3-methyl)butyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-5-(N-cyclohexylaminocarbonyl-methylamino)-1-[4-[(1-methoxycarbonyl-3-methyl)butyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 72.9% of theory, Melting point: 178.degree.-182.degree. C. C.sub.32 H.sub.44 N.sub.4 O.sub.4 (548.73) Calculated: C 70.04 H 8.08 N 10.21 Found: 69.77 7.97 10.04 Mass spectrum: m/e=548
EXAMPLE 48
2-n-Butyl-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-5-methyl-6-dimethylaminocarbonylamino-imidazo[4,5-b]pyridine
Prepared analogously to Example 1b from 2-n-butyl-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-5-methyl-6-dimethylaminocarbonylamino-imidazo[4,5-b]pyridine and 2N sodium hydroxide solution in ethanol.
Yield: 80.0% of theory, Melting point: 191.degree.-193.degree. C. C.sub.29 H.sub.33 N.sub.5 O.sub.4 (515.62) Calculated: C 67.55 H 6.45 N 13.58 Found: 67.49 6.62 13.57
EXAMPLE 49
2-n-Butyl-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-5-methyl-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinon-1-yl)imidazo[4,5-b]pyridine
Prepared analogously to Example 1b from 2-n-butyl-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-5-methyl-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinon-1-yl)-imidazo[4,5-b]pyridine and 2N sodium hydroxide solution in ethanol.
Yield: 87.5% of theory, Melting point: 157.degree.-160.degree. C. C.sub.37 H.sub.39 N.sub.5 O.sub.4 (617.75) Calculated: C 71.94 H 6.36 N 11.34 Found: 71.71 6.36 10.96
EXAMPLE 50
2-n-Butyl-6-(N-methylaminocarbonyl-n-pentylamino)-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-6-(N-methylaminocarbonyl-n-pentylamino)-1-[4-[(.alpha.-methoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 76.0% of theory, Melting point: 170.degree.-172.degree. C. C.sub.33 H.sub.40 N.sub.4 O.sub.4 (556.71) Calculated: C 71.20 H 7.24 N 10.07 Found: 70.80 7.34 9.96
EXAMPLE 51
2-n-Butyl-6-(N-cyclohexylaminocarbonyl-n-pentylamino)-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-6-(N-cyclohexylaminocarbonyl-n-pentylamino)-1-[4-[(.alpha.-methoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 65.0% of theory, Melting point: 83.degree. C. C.sub.38 H.sub.48 N.sub.4 O.sub.4 (624.83) Calculated: C 73.05 H 7.74 N 8.97 Found: 72.80 7.51 8.59
EXAMPLE 52
2-n-Butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-carboxy-.alpha.-phenyl)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-4-hydroxymethyl-5-chloro-1-[4-[(.alpha.-methoxycarbonyl-.alpha.-phenyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 50.0% of theory, Melting point: 165.degree. C. (decomp.) C.sub.29 H.sub.29 ClN.sub.2 O.sub.4 (505.02) Calculated: C 68.97 H 5.79 N 5.55 Found: 68.50 5.72 5.51
EXAMPLE 53
2-n-Butyl-6-(p-methyl-phenylsulphonylamino)-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-6-(p-methyl-phenylsulphonylamino)-1-[4-[(.alpha.-methoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 81.0% of theory, Melting point: 150.degree. C. C.sub.33 H.sub.33 N.sub.3 O.sub.5 S (583.71) Calculated: C 67.90 H 5.70 N 7.20 Found: 67.18 5.70 6.96
EXAMPLE 54
2-n-Butyl-6-(N-methyl-p-methyl-phenylsulphonylamino)-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-6-(N-methyl-p-methyl-phenylsulphonylamino)-1-[4-[(.alpha.-methoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 96.0% of theory, Melting point: 218.degree. C. C.sub.34 H.sub.35 N.sub.3 O.sub.5 S (597.71) Calculated: C 68.32 H 5.90 N 7.03 Found: 67.82 5.84 6.85
EXAMPLE 55
2-n-Butyl-6-(N-n-pentyl-p-methyl-phenylsulphonylamino)-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-6-(N-n-pentyl-p-methyl-phenylsulphonylamino)-1-[4-[(.alpha.-methoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 94.0% of theory, Melting point: 202.degree. C. C.sub.38 H.sub.43 N.sub.3 O.sub.5 S (653.81) Calculated: C 69.80 H 6.63 N 6.43 Found: 69.47 6.57 6.64
EXAMPLE 56
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]-(.alpha.-methyl)-benzyl]benzimidazole
a) 4-[(.alpha.-Ethoxycarbonyl)benzyloxy]acetophenone
Prepared analogously to Example 1a from ethyl 2-bromophenylacetate and 4-hydroxyacetophenone.
Yield: 78.0% of theory, R.sub.f value: 0.58 (silica gel; eluant: toluene/ethyl acetate=85:15)
b) 1-[4-[(.alpha.-Ethoxycarbonyl)benzyloxy]phenyl]ethanol
2.3 g (7.7 mMol) of 4-[(.alpha.-ethoxycarbonyl)benzyloxy]-acetophenone are dissolved in 50 ml of ethanol and at ambient temperature 0.28 g (7.7 mMol) of sodium borohydride are added thereto in batches. The reaction mixture is stirred at 40.degree. C. for 2 hours. After cooling to ambient temperature it is concentrated by evaporation and the residue is mixed with water and dilute hydrochloric acid. After extraction with ethyl acetate the organic phase is dried with sodium sulphate. The solution is evaporated down and the residue is chromatographed over a silica gel column (particle size: 0.063-0.02 mm) using toluene/ethyl acetate (7:3). The uniform fractions are combined and evaporated down.
Yield: 1.0 g (43% of theory), Oil, R.sub.f value: 0.50 (silica gel; eluant: toluene/ethyl acetate=7:3)
c) 1-[4-[(.alpha.-Ethoxycarbonyl)benzyloxy]phenyl]-1-methanesulphonyloxyethan
0.5 g (1.7 mMol) of 1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]phenyl]ethanol and 0.22 g (2.2 mMol) of triethylamine are dissolved in 10 ml of methylene chloride. At 5.degree. C., 0.23 g (2.0 mMol) of mesyl chloride are added dropwise with stirring. After one hour at ambient temperature the reaction mixture is poured onto ice water, extracted with methylene chloride and dried over sodium sulphate. The crude product obtained is reacted directly without any further purification.
d) 2-n-Butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-(.alpha.-methyl)benzyl]benzimidazole
A mixture of 0.26 g (1.5 mMol) of 2-n-butyl-benzimidazole, 3 ml (1.7 mMol) of triethylamine and 1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]phenyl]-1-methanesulphonyloxyethane (crude) is heated to 80.degree. C. for 30 minutes. After cooling, water is added and the mixture is extracted twice with ethyl acetate. The organic phases are combined, dried and evaporated down. The crude product is chromatographed over a silica gel column (particle size: 0.063-0.02 mm) with toluene/ethyl acetate (8:2). The uniform fractions are combined and evaporated down.
Yield: 0.10 g (15% of theory), R.sub.f value: 0.35 (silica gel; eluant: toluene/ethyl acetate=8:2)
e) 2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]-(.alpha.-methyl)-benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-(.alpha.-methyl)benzyl]-benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 82% of theory, Melting point: 109.degree. C. C.sub.27 H.sub.28 N.sub.2 O.sub.3 (428.51) Calculated: C 75.67 H 6.59 N 6.54 Found: 75.93 6.76 6.32
EXAMPLE 57
2-n-Butyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)pyrimidinon-1-yl)-benzimidazole
a) 5-Benzyl-1H-tetrazole
Prepared from benzylcyanide and sodium azide/ammonium chloride in dimethylformamide.
Yield: 70% of theory, Melting point: 130.degree.-132.degree. C.
b) N-Triphenylmethyl-5-benzyl-tetrazole
Prepared from 5-benzyl-1H-tetrazole and triphenylmethyl-chloride.
Yield: 84% of theory, Melting point: 158.degree.-160.degree. C.
c) N-Triphenylmethyl-5-(.alpha.-bromo)benzyl-tetrazole
2.0 g (5 mMol) of N-triphenylmethyl-5-benzyl-tetrazole, 0.89 g (5 mMol) of N-bromosuccinimide and 30 mg of azo-bisisobutyronitrile are dissolved in 25 ml of carbon tetrachloride and heated to boiling for one hour under UV-irradiation. After cooling, the succinimide formed is filtered off and the filtrate is evaporated down.
Yield: 74.6% of theory, Melting point: 160.degree.-162.degree. C.
d) 2-n-Butyl-1-[(4-benzyloxy)benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)pyrimidinon-1-yl)-benzimidazole
Prepared analogously to Example 1a from 2-n-butyl-6-(3-benzyl3,4,5,6-tetrahydro-2(1H)pyrimidinon-1-yl)-benzimidazole and 4-benzyloxybenzyl chloride.
Yield: 65.5% of theory, R.sub.f value: 0.45 (silica gel; eluant: methylene chloride/ethanol=19:1)
e) 2-n-Butyl-1-[(4-hydroxy)benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)pyrimidinon-1-yl)-benzimidazole
2.4 g of 2-n-butyl-1-[(4-benzyloxy)benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)pyrimidinon-1-yl)-benzimidazole are dissolved in 100 ml of ethanol and 30 ml of dimethylformamide, mixed with 2.4 g of (10%) palladium on activated charcoal and hydrogenated with 5 bars of hydrogen at ambient temperature. The catalyst is filtered off, the filtrate is evaporated down and the residue is chromatographed over a silica gel column (particle size: 0.063-0.02 mm) with methylene chloride/ethanol. The uniform fractions are combined and evaporated down and the residue is triturated with ether and suction filtered.
Yield: 1.1 g (55% of theory), Melting point: 190.degree.-191.degree. C.
f) 2-n-Butyl-1-[4-[(.alpha.-(N-triphenylmethyl)tetrazol-5-yl)-benzyloxy]benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H) pyrimidinon-1-yl) -benzimidazole
0.5 g (1.1 mMol) of 2-n-butyl-1-[(4-hydroxy)benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H) pyrimidinon-1-yl) -benzimidazole, 0.74 g (5.4 mMol) of potassium carbonate and 0.93 g (1.1 mMol) of N-triphenylmethyl-5-[(.alpha.-bromo)-benzyl]tetrazole are dissolved in 10 ml of dimethylsulphoxide and stirred for 2 hours at ambient temperature. The product is precipitated by the addition of saline solution, suction filtered, washed with water and dried.
Yield: 0.9 g (97% of theory), Melting point: from 150.degree. C. (decomp.)
g) 2-n-Butyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)-pyrimidin-1-yl)-benzimidazole
0.9 g (1.0 mMol) of 2-n-butyl-1-[4-[(.alpha.-N-triphenyl-methyl)tetrazol-5-yl)benzyloxy]benzyl]-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)pyrimidinon-1-yl)-benzimidazole are dissolved in 40 ml of ethanol, 10 ml of methylene chloride and 12 ml of 4N hydrochloric acid and stirred for 2 hours at ambient temperature. After the addition of 30 ml of water, the mixture is extracted with methylene chloride. The organic phase is washed with 1N sodium hydroxide solution, acidified with dilute acetic acid and dried over sodium sulphate. The crude product is chromatographed over a silica gel column (particle size: 0.063-0.02 mm) with methylene chloride/ethanol (50:1 and 19:1). The unified fractions are combined and evaporated down.
Yield: 0.22 g (34% of theory), Melting point: from 134.degree. C. (decomp.) C.sub.37 H.sub.38 N.sub.8 O.sub.2 (626.76) Calculated: C 70.90 H 6.11 N 17.88 Found: 70.72 6.00 17.68
EXAMPLE 58
2-n-Propyl-4-methyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole
a) 2-n-Propyl-4-methyl-1-[4-[.alpha.-((N-triphenylmethyl)-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 57f from 2-n-propyl-4-methyl-1-[(4-hydroxy)benzyl]benzimidazole and N-triphenylmethyl-5-[(.alpha.-bromo)benzyl]tetrazole.
Yield: 99% of theory, R.sub.f value: 0.68 (silica gel; eluant: methylene chloride/methanol=19:1)
b) 2-n-Propyl-4-methyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)-benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 57g from 2-n-propyl-4-methyl-1-[4-[.alpha.-((N-triphenylmethyl)tetrazol-5-yl)-benzyloxy]benzyl]benzimidazole and methanolic hydrochloric acid.
Yield: 63% of theory, Melting point: amorphous C.sub.26 H.sub.26 N.sub.6 O (438.54) Calculated: C 71.21 H 5.98 N 19.17 Found: 70.99 5.98 18.96
EXAMPLE 59
2-n-Propyl-4-methyl-6-(1-methyl-benzimidazol-2-yl)-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole
a) 2-n-Propyl-4-methyl-6-(1-methyl-benzimidazol-2-yl)-1-[4-[.alpha.-((N-triphenylmethyl)tetrazol-5-yl)benzyloxy]-benzyl]benzimidazole
Prepared analogously to Example 57f from 2-n-propyl-4-methyl-6-1-benzimidazol-2-yl)-1-[(4-hydroxy)-benzyl]benzimidazole (synthesised analogously to Examples 58d and 58e) and N-triphenylmethyl-5-(.alpha.-bromo)-benzyl-tetrazole.
Yield: 94% of theory, R.sub.f value : 0.70 (silica gel; eluant: methylene chloride/methanol=9:1)
2-n-Propyl-4-methyl-6-(1-methyl-benzimidazol-2-yl)-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl-benzimidazole
Prepared analogously to Example 57g from 2-n-propyl-4-methyl-6-(1-methyl-benzimidazol-2-yl)-1-[4-[.alpha.-((N-triphenylmethyl)-tetrazol-5-yl)benzyloxy]benzyl]-benzimidazole and methanolic hydrochloric acid.
Yield: 62% of theory, Melting point: 165.degree.-166.degree. C. C.sub.34 H.sub.32 N.sub.8 O (568.69) Calculated: C 71.81 H 5.67 N 19.71 Found: 71.68 5.60 19.55
EXAMPLE 60
2-n-Propyl-5-n-butyrylamino-3-[4-[(1-(1H-tetrazol-5-yl)-3-methyl)butyloxy]benzyl]imidazo[4,5-b]pyridine
a) 2-n-Propyl-5-n-butyrylamino-3-[4-[(1-cyano-3-methyl)-butyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 57f from 2-n-propyl-5-n-butyrylamino-3-[(4-hydroxy)benzyl]imidazo[4,5-b]-pyridine and 2-bromo-4-methyl-valeronitrile.
Yield: 85% of theory, R.sub.f value: 0.40 (silica gel; eluant: ethyl acetate/petroleum ether=1:1)
b) 2-n-Propyl-5-n-butyrylamino-3-[4-[(1-(1H-tetrazol-5-yl)-3-methyl)butyloxy]benzyl]imidazo[4,5-b]pyridine
A mixture of 800 mg (1.78 mMol) of 2-n-propyl-5-n-butyrylamino-3-[4-[(1-cyano-3-methyl)butyloxy)benzyl]-imidazo[4,5-b]pyridine, 2.2 g (3.3 mMol) of sodium azide and 1.8 g (3.3 mMol) of ammonium chloride in 8 ml of dimethylformamide is stirred for 5 hours at 130.degree. C. with stirring. It is then stirred into ice water, the product precipitated is suction filtered, washed with water, dried and purified over a silica gel column (methylene chloride/ethanol=19:1). The fractions containing the desired substance are evaporated down, the residue obtained is triturated with ether, suction filtered and dried. When the purification step described above is repeated, 370 mg (42% of theory) of the product are obtained, m.p. 175.degree.-177.degree. C.
C.sub.26 H.sub.34 N.sub.8 O.sub.2 (490.61) Calculated: C 63.65 H 6.99 N 22.84 Found: 63.61 7.03 22.83
EXAMPLE 61
2-n-Butyl-4-hydroxymethyl-5-chloro-1-[4-[1-(1H-tetrazol-5-yl)propyloxy]benzyl]benzimidazole sesqui-hydrochloride
Prepared analogously to Example 60b from 2-n-butyl-4-hydroxymethyl-5-chloro-1-[4-(1-cyano-propyloxy)benzyl]-imidazole and sodium azide/ammonium chloride in dimethylformamide.
Yield: 78% of theory, Oil, C.sub.19 H.sub.25 ClN.sub.6 O.sub.2 .times.1.5 HCl (459.59) Calculated: C 49.65 H 5.81 18.28 Found: 49.41 6.03 18.52
EXAMPLE 62
2-n-Butyl-5-hydroxymethyl-4-chloro-1-[4-[1-(1H-tetrazol-5-yl)propyloxy]benzyl]benzimidazole sesqui-hydrochloride
Prepared analogously to Example 60b from 2-n-butyl-5-hydroxymethyl-4-chloro-1-[4-(1-cyano-propyloxy)benzyl]-imidazole and sodium azide/ammonium chloride in dimethylformamide.
Yield: 70% of theory, Oil, C.sub.19 H.sub.25 ClN.sub.6 O.sub.2 .times.1.5 HCl (459.59) Calculated: C 49.65 H 5.81 N 18.28 Found: 49.86 5.76 18.26
EXAMPLE 63
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzylamino]benzyl]-benzimidazole semihydrate
a) 2-n-Butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzylamino]-benzyl]benzimidazole
1.0 g (3.6 mMol) of 2-n-butyl-1-[(4-amino)benzyl]-benzimidazole, 0.87 g (3.6 mMol) of ethyl 2-bromo-phenylacetate and 0.5 g of sodium acetate-trihydrate are dissolved in 20 ml of ethanol and stirred for 18 hours at ambient temperature. Then the reaction mixture is refluxed for a further 4 hours. The solvent is evaporated down, water is added to the residue which is made alkaline with dilute ammonia solution and extracted with ethyl acetate. The combined organic phases are washed with water, dried over magnesium sulphate and concentrated by evaporation. The residue is purified over a silica gel column (particle size: 0.063-0.02 mm), using petroleum ether with 10-15% ethyl acetate as eluant. The uniform fractions are combined and evaporated down.
Yield: 1.0 g (63% of theory), R.sub.f value: 0.53 (silica gel; eluant: petroleum ether/ethyl acetate=3:1)
b) 2-n-Butyl-1-[4-[(.alpha.-carboxy)benzylamino]benzyl]-benzimidazole semihydrate
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzylamino]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 99% of theory, C.sub.26 H.sub.27 N.sub.3 O.sub.2 .times.0.5 H.sub.2 O (422.54) Calculated: C 73.91 H 6.68 N 9.95 Found: 74.05 6.55 9.91
EXAMPLE 64
2-n-Butyl-1-[4-[(.alpha.-carboxy)-N-acetyl-benzylamino]benzyl]benzimidazole
a) 2-n-Butyl-1-[4-[(.alpha.-ethoxycarbonyl)-N-acetyl-benzylamino]benzyl]benzimidazole
0.5 g (1.1 mMol) of 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzylamino]benzyl]benzimidazole are dissolved in 5 ml of aceticanhydride and stirred at 120.degree. C. for 3 hours. The solvent is eliminated, the residue is purified over a silica gel column (particle size: 0.063-0.02 mm), using ethyl acetate as eluant. The uniform fractions are combined and evaporated down.
Yield: 0.35 g (64% of theory), R.sub.f value: 0.50 (silica gel; eluant: methylene chloride/ethanol=95:5)
b) 2-n-Butyl-1-[4-[(.alpha.-carboxy)-N-acetyl-benzylamino]-benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)-N-acetyl-benzylamino]benzyl]-benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 74% of theory, Melting point: 212.degree.-214.degree. C. C.sub.28 H.sub.29 N.sub.3 O.sub.3 (455.56) Calculated: C 73.82 H 6.42 N 9.12 Found: 73.50 6.58 9.28
EXAMPLE 65
2-n-Butyl-1-[4-[(2-carboxy-3-phenyl)propyl]benzyl]-benzimidazole
a) 2-n-Butyl-1-[(4-hydroxymethyl)benzyl]benzimidazole
1.2 g (30 mMol) of lithium aluminium hydride are suspended in 200 ml of absolute tetrahydrofuran. At ambient temperature a solution of 10.6 g (30 mMol) of 2-n-butyl-1-[(4-ethoxycarbonyl)benzyl]benzimidazole in 100 ml of absolute tetrahydrofuran is added dropwise. The mixture is stirred for 2 hours at ambient temperature and then slowly hydrolysed, with cooling, with aqueous sodium hydroxide solution. The crystallised salts are suction filtered and the filtrate is concentrated by evaporation. The residue is mixed with water and extracted with ethyl acetate. The combined organic phases are washed with water, dried and concentrated by evaporation. The residue is purified over a silica gel column (particle size: 0.063-0.02 mm), using methylene chloride/methanol (30:1) as eluant. The uniform fractions are combined and evaporated down.
Yield: 6.5 g (74% of theory), R.sub.f value: 0.60 (silica gel; eluant: methylene chloride/methanol=19:1)
b) 2-n-Butyl-1-[(4-chloromethyl)benzyl]benzimidazole
6.2 g (21 mMol) of 2-n-butyl-1-[(4-hydroxymethyl)benzyl]benzimidazole are dissolved in 10 ml of thionylchloride and refluxed for 10 minutes. Then the excess thionylchloride is distilled off and the residue is mixed with ice water. After neutralisation with saturated sodium hydrogen carbonate solution, the mixture is extracted with ethyl acetate. The combined organic phases are washed with water, dried and evaporated down.
Yield: 6.0 g (91% of theory), R.sub.f value: 0.65 (silica gel; eluant: methylene chloride/methanol=19:1)
c) 2-n-Butyl-1-[4-[(2,2-bis-ethoxycarbonyl-3-phenyl)-propyl]benzyl]benzimidazole
2.4 g (9.6 mMol) of diethyl benzylmalonate are dissolved in 25 ml of dimethylsulphoxide, mixed with 1.1 g (9.6 mMol) of potassium tert.butoxide and stirred for 30 minutes at ambient temperature. Then a solution of 3.0 g (9.6 mMol) of 2-n-butyl-1-[(4-chloromethyl)-benzyl]benzimidazole in 25 ml of dimethylsulphoxide is added dropwise. After one hour at ambient temperature the mixture is heated to 100.degree. C. for 15 minutes. The reaction mixture is combined with ice water. The product which crystallises out is suction filtered and extracted with ethyl acetate. The combined organic phases are washed with water, dried and evaporated down.
Yield: 4.2 g (83% of theory), R.sub.f value: 0.15 (silica gel; eluant: methylene chloride/methanol=30:1)
d) 2-n-Butyl-1-[4-[(2-carboxy-3-phenyl)propyl]benzyl]-benzimidazole
0.44 g (0.8 mMol) of 2-n-butyl-1-[4-[(2,2-bis-ethoxycarbonyl-3-phenyl)propyl]benzyl]benzimidazole and 0.12 g of sodium hydroxide are taken up in 30 ml of water and refluxed for 8 hours. The mixture is then acidified with glacial acetic acid. The product which crystallises out is suction filtered and purified over a silica gel column (particle size: 0.063-0.02 mm), using methylene chloride/methanol (19:1) as eluant. The uniform fractions are combined, evaporated down, triturated with ether, suction filtered and dried.
Yield: 0.1 g (39% of theory), Melting point: 139.degree.-140.degree. C. C.sub.28 H.sub.30 N.sub.2 O.sub.2 (426.56) Calculated: C 78.84 H 7.09 N 6.57 Found: 78.72 6.96 6.56
EXAMPLE 66
2-n-Butyl-1-[4-[(2-carboxy-2-phenyl)ethyl]benzyl]-benzimidazole
a) 2-n-Butyl-1-[4-[(2,2-bis-ethoxycarbonyl-2-phenyl)-ethyl]benzyl]benzimidazole
Prepared analogously to Example 65c from 2-n-butyl-1-[(4-chloromethyl)benzyl]benzimidazole and diethylphenylmalonate/potassium tert.butoxide.
Yield: 82% of theory, R.sub.f value: 0.20 (silica gel; eluant: methylene chloride/methanol=40:1)
b) 2-n-Butyl-1-[4-[(2-carboxy-2-phenyl)ethyl]-benzyl]benzimidazole
Prepared analogously to Example 65d from 2-n-butyl-1-[4-[(2,2-bis-ethoxycarbonyl-2-phenyl)ethyl]benzyl]-benzimidazole and aqueous sodium hydroxide solution in ethanol.
Yield: 37% of theory, Melting point: 171.degree.-172.degree. C. C.sub.27 H.sub.28 N.sub.2 O.sub.2 (412.54) Calculated: C 78.44 H 6.66 N 6.88 Found: 78.61 6.84 6.79
EXAMPLE 67
2-n-Propyl-5-(N-ethyl-cyclohexylcarbonylamino)-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 1b from 2-n-propyl-5-(N-ethylcyclohexylcarbonylamino)-3-[4-[(.alpha.-ethoxycarbonyl)-benzyl]imidazo[4,5-b]pyridine and 1N sodium hydroxide solution in ethanol.
Yield: 87% of theory, Melting point: 113.degree.-115.degree. C. C.sub.33 H.sub.38 N.sub.4 O.sub.4 (554.70) Calculated: C 71.46 H 6.91 N 10.10 Found: 71.60 6.99 10.00
EXAMPLE 68
2-n-Propyl-5-(N-cyclohexylaminocarbonyl-ethylamino)-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 1b from 2-n-propyl-5-(N-cyclohexylaminocarbonyl-ethylamino)-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 20% of theory, Melting point: 183.degree. C. C.sub.33 H.sub.39 N.sub.5 O.sub.4 (569.72) Calculated: C 69.57 H 6.90 N 12.29 Found: 69.80 6.69 11.97
EXAMPLE 69
2-n-Butyl-4-methyl-7-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-4-methyl-7-[.alpha.-(1-triphenylmethyl-tetrazol-5-yl)benzyloxy]-1-[4-[.alpha.-((1-triphenylmethyl)tetrazol-5 -yl)benzyloxy]-benzyl]benzimidazole and 4N hydrochloric acid.
Yield: 52% of theory, Melting point: from 166.degree. C. (decomp.) C.sub.35 H.sub.34 N.sub.10 O.sub.2 (626.72) Calculated: C 67.68 H 5.46 N 22.35 Found: 67.70 5.52 22.66
EXAMPLE 70
2-n-Butyl-5-methyl-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinon-1-yl)-3-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 57g from 2-n-butyl-5-methyl-6-(3-benzyl-3,4,5,6-tetrahydro-2(1H)pyrimidinon-1-yl)-3-[4-[.alpha.-((1-triphenylmethyl)tetrazol-5-yl) benzyloxy]benzyl]imidazo[4,5-b]pyridine and 4N hydrochloric acid.
Yield: 75% of theory, Melting point: 185.degree.-187.degree. C. C.sub.37 H.sub.39 N.sub.9 O.sub.2 (661.79) Calculated: C 69.24 H 6.12 N 19.64 Found: 68.97 6.17 19.91
EXAMPLE 71
2-n-Butyl-5-dimethylaminocarbonylamino-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-5-dimethylaminocarbonylamino-1-[4-[.alpha.-((1-triphenylmethyl)-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole and 4N hydrochloric acid.
Yield: 66% of theory, Melting point: 207.degree.-209.degree. C. C.sub.29 H.sub.32 N.sub.8 O.sub.2 (524.68) Calculated: C 64.19 H 6.31 N 20.65 Found: 63.95 6.33 20.44
EXAMPLE 72
2-Ethyl-5,7-dimethyl-3-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]imidazo[4,5-b]pyridine semihydrate
Prepared analogously to Example 57g from 2-ethyl-5,7-dimethyl-3-[4-[.alpha.-((1-triphenylmethyl)tetrazol-5-yl)benzyloxy]benzyl]imidazo[4,5-b]pyridine-semihydrate and 4N hydrochloric acid.
Yield: 83% of theory, Melting point: 104.degree.-105.degree. C. C.sub.25 H.sub.25 N.sub.7 O.times.0.5 H.sub.2 O (448.53) Calculated: C 66.95 H 5.89 N 21.83 Found: 67.05 5.94 22.36
EXAMPLE 73
2-n-Propyl-4-methyl-1-[4-[.alpha.-(O-ethyl-phosphono)benzylamino]benzyl]benzimidazole hydrate
a) 2-n-Propyl-4-methyl-1-[4-[.alpha.-(O,O-diethyl-phosphono)-benzylamino]benzyl]benzimidazole
6.5 g (23.3 mMol) of 2-n-propyl-4-methyl-1-[(4-amino)benzyl]benzimidazole are stirred into 4.2 g (40 mMol) of benzaldehyde. The mixture is stirred at ambient temperature for 2 hours. Then 10.72 g (77.6 mMol) of diethylphosphite are added and the mixture is heated to 100.degree. C. for 45 minutes. The precipitate formed after cooling to ambient temperature is triturated with 250 ml of ether, suction filtered and dried in air. It is then recrystallised from 150 ml of petroleum ether/isopropanol (2:1).
Yield: 8.7 g (74% of theory), Melting point: 110.degree.-115.degree. C.
b) 2-n-Propyl-4-methyl-1-[4-[.alpha.-(O-ethyl-phosphono)-benzylamino]benzyl]benzimidazole hydrate
0.5 g (1 mMol) of 2-n-propyl-4-methyl-1-[4-[.alpha.-(O,O-diethylphosphono)benzylamino]benzyl]benzimidazole and 1.0 g of pulverised potassium hydroxide are dissolved in 20 ml of methanol and refluxed for 9 hours. After cooling to ambient temperature, 25 ml of ice water are added. The pH is adjusted to 6.5 by the addition of glacial acetic acid and conc. ammonia solution. After the addition of solid sodium chloride, extraction is carried out six times, each time using 100 ml of ethyl acetate. The combined organic phases are washed with saturated saline solution and dried over magnesium sulphate. After evaporation of the solvent in vacuo the residue is triturated with ether and dried over potassium hydroxide.
Yield: 0.35 g (73% of theory), Melting point: from 158.degree. C. (decomp.) C.sub.27 H.sub.32 N.sub.3 O.sub.3 P.times.H.sub.2 O (495.57) Calculated: C 65.44 H 6.92 N 8.48 Found: 65.66 6.52 8.49
EXAMPLE 74
2-n-Butyl-1-[4-[.alpha.-(O-ethyl-phosphono)benzylamino]benzyl]benzimidazole
a) 2-n-Butyl-1-[4-[.alpha.-(O,O-diethyl-phosphono)-benzylamino]benzyl]benzimidazole
Prepared analogously to Example 73a from 2-n-butyl-1-[(4-amino)benzyl]benzimidazole, benzaldehyde and diethylphosphite.
Yield: 51% of theory, Melting point: 149.degree.-153.degree. C.
b) 2-n-Butyl-1-[4-[.alpha.-(O-ethyl-phosphono)benzylamino]benzyl]benzimidazole
Prepared analogously to Example 73b from 2-n-butyl-1-[4-[.alpha.-(O,O-diethyl-phosphono)benzylamino]benzyl]-benzimidazole and methanolic potassium hydroxide solution.
Yield: 67% of theory, Melting point: 118.degree.-123.degree. C. (decomp.) C.sub.27 H.sub.32 N.sub.3 O.sub.3 P (477.55) Calculated: C 67.91 H 6.76 N 8.80 Found: 67.74 7.02 8.64
EXAMPLE 75
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-benzimidazole
a) 2-n-Butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-benzyl]benzimidazole
Prepared analogously to Example 1a from 2-n-butyl-benzimidazole and 4-[(.alpha.-ethoxycarbonylbenzyloxy)-benzyl]bromide.
Yield: 22.0% of theory, Oil, R.sub.f value: 0.65 (silica gel; eluant: ethyl acetate/ethanol=9:1) C.sub.28 H.sub.30 N.sub.2 O.sub.3 (442.60) Calculated: C 75.99 H 6.83 N 6.33 Found: 75.97 6.79 5.99
b) 2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 38.0% of theory, Melting point: 223.degree.-225.degree. C. C.sub.26 H.sub.26 N.sub.2 O.sub.3 (414.51) Calculated: C 75.34 H 6.32 N 6.76 Found: 75.03 6.34 6.73
EXAMPLE 76
2-n-Propyl-7-methyl-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-imidazo[4,5-b]pyridine
a) 2-n-Propyl-7-methyl-3-[4-[(.alpha.-ethoxycarbonyl)-benzyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 1a from 2-n-propyl-7-methylimidazo[4,5-b]pyridine and 4-[(.alpha.-ethoxycarbonyl)-benzylbromide.
Yield: 34.0% of theory, Oil, R.sub.f value: 0.40 (silica gel; eluant: methylene chloride/ethanol=25:1)
b) 2-n-Propyl-7-methyl-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazo[4,5-b]pyridine
Prepared analogously to Example 1b from 2-n-propyl-7-methyl-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-imidazo[4,5-b]pyridine and 1N sodium hydroxide solution in ethanol.
Yield: 66.4% of theory, Melting point: 108.degree. C. C.sub.25 H.sub.25 N.sub.3 O.sub.3 (415.50) Calculated: C 72.27 H 6.06 N 10.11 Found: 72.25 6.03 9.87
EXAMPLE 77
5,7-Dimethyl-2-ethyl-3-[4-[(.alpha.-carboxy)benzyloxy]benzyl]imidazo[4,5-b]pyridine semihydrate
Prepared analogously to Example 1b from 5,7-dimethyl-2-ethyl-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-imidazo[4,5-b]pyridine and 1N sodium hydroxide solution in ethanol.
Yield: 89.3% of theory, Melting point: 251.degree.-253.degree. C. C.sub.25 H.sub.25 N.sub.3 O.sub.3 .times.0.5 H.sub.2 O (424.50) Calculated: C 70.73 H 6.17 N 9.89 Found: 71.00 5.97 9.79
EXAMPLE 78
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]-3,5-dichloro-benzyl]-6-cyclohexylaminocarbonylamino-benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-3,5-dichlorobenzyl]-6-cyclohexylaminocarbonylamino-benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 95.6% of theory, Melting point: 251.degree.-253.degree. C. C.sub.33 H.sub.36 Cl.sub.2 N.sub.4 O.sub.4 (623.58) Calculated: C 63.56 H 5.82 N 8.98 C.sub.1 11.37 Found: 63.39 5.88 8.87 11.85
EXAMPLE 79
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]-3-methoxybenzyl]-6-dimethylaminocarbonylamino-benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-3-methoxybenzyl]-6-dimethylaminocarbonylamino-benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 87.5% of theory, Melting point: 170.degree.-172.degree. C. C.sub.30 H.sub.34 N.sub.4 O.sub.5 (530.62) Calculated: C 67.90 H 6.46 N 10.56 Found: 67.64 6.25 10.33
EXAMPLE 80
2-n-Butyl-1-[4-[(.alpha.-carboxy)benzyloxy]-3-methoxybenzyl]-5-dimethylaminocarbonylamino-benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-3-methoxybenzyl]-5-dimethylaminocarbonylamino-benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 78.6% of theory, Melting point: 154.degree.-156.degree. C. C.sub.30 H.sub.34 N.sub.4 O.sub.5 (530.62) Calculated: C 67.90 H 6.46 N 10.56 Found: 67.74 6.37 10.24
EXAMPLE 81
2-n-Propyl-1-[4-[(.alpha.-carboxy)benzyloxy]-3,5-dimethoxybenzyl]-6-(1-methyl-benzimidazol-2-yl)-4-methyl-benzimidazole hydrate
Prepared analogously to Example 1b from 2-n-propyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-3,5-dimethoxybenzyl]-6-(1-methylbenzimidazol-2-yl)-4-methyl-benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 91.4% of theory, Melting point: 148.degree.-150.degree. C. C.sub.36 H.sub.36 N.sub.4 O.sub.5 .times.H.sub.2 O (622.72) Calculated: C 69.44 H 6.25 N 9.00 Found: 69.77 6.09 8.92
EXAMPLE 82
2-n-Propyl-1-[4-[(.alpha.-carboxy)benzyloxy]-3,5-dibromobenzyl]-6-(1-methyl-benzimidazol-2-yl)-4-methyl-benzimidazole hydrate
Prepared analogously to Example 1b from 2-n-propyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-3,5-dibromobenzyl]-6-(1-methyl-benzimidazol-2-yl)-4-methyl-benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 58.4% of theory, Melting point: 229.degree.-230.degree. C. C.sub.34 H.sub.30 Br2N.sub.4 O.sub.3 .times.H.sub.2 O (720.46) Calculated: C 56.68 H 4.47 N 7.77 Found: 56.78 4.64 7.75
EXAMPLE 83
2-n-Propyl-6-(2,3-dimethylsuccinimino)-1-[4-[(.alpha.-carboxy)benzyloxy]-3,5-dimethoxybenzyl]-benzimidazole semihydrate
Prepared analogously to Example 1b from 2-n-propyl-6-(2,3-dimethylsuccinimino)-1-[4-[(.alpha.-ethoxycarbonyl)-benzyloxy]-3,5-dimethoxybenzyl]-benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 13.8% of theory, Melting point: 129.degree.-131.degree. C. C.sub.34 H.sub.37 N.sub.3 O.sub.7 .times.0.5 H.sub.2 O (608.69) Calculated: C 67.09 H 6.29 N 6.90 Found: 67.29 6.37 6.86
EXAMPLE 84
2-n-Butyl-6-(1-cyclohexen-1,2-dicarbonylimino)-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole semihydrate
Prepared analogously to Example 1b from 2-n-butyl-6-(1-cyclohexen-1,2-dicarbonylimino)-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole and 1N sodium hydroxide solution in ethanol.
Yield: 23.3% of theory, Melting point: 247.degree.-249.degree. C. C.sub.34 H.sub.33 N.sub.3 O.sub.5 .times.0.5 H.sub.2 O (572.66) Calculated: C 71.30 H 5.98 N 7.33 Found: 71.47 6.05 7.15
EXAMPLE 85
2-n-Propyl-4-methyl-1-[4-[(.alpha.-carboxy)-2-chlorobenzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-propyl-4-methyl-1-[4-[(.alpha.-ethoxycarbonyl)-2-chlorobenzyloxy]-benzyl]benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 40.7% of theory, Melting point: sinters from 105.degree. C. C.sub.26 H.sub.25 ClN.sub.2 O.sub.3 (448.97) Calculated: C 70.00 H 5.62 N 6.28 Cl 7.91 Found: 69.94 5.72 6.29 7.92
EXAMPLE 86
2-n-Propyl-4-chloro-6-(1-oxo-2-isoindolin-2-yl)-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-n-propyl-4-chloro-6-(1-oxo-2-isoindolin-2-yl)-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 61.0% of theory, Melting point: 252.degree.-254.degree. C. C.sub.33 H.sub.28 ClN.sub.3 O.sub.4 (566.06) Calculated: C 70.00 H 5.02 N 7.42 Cl 6.25 Found: 69.81 5.22 7.87 6.54
EXAMPLE 87
2-n-Butyl-6-(1-cyclohexen-1,2-dicarbonylimino)-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-6-(1-cyclohexen-1,2-dicarbonylimino)-1-[4-[(.alpha.-(N-triphenylmethyl)tetrazol-5-yl)benzyloxy]benzyl]-benzimidazole and 85% formic acid in methylene chloride.
Yield: 43.7% of theory, Melting point: 149.degree.-151.degree. C. C.sub.34 H.sub.33 N.sub.7 O.sub.3 (587.68) Calculated: C 69.49 H 5.66 N 16.68 Found: 69.40 5.83 16.31
EXAMPLE 88
2-n-Butyl-5-methyl-6-dimethylaminocarbonylamino-3-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]imidazol[4,5-b]-pyridine
Prepared analogously to Example 57g from 2-n-butyl-5-methyl-6-dimethylaminocarbonylamino-3-[4-[(.alpha.-(N-triphenylmethyl)tetrazol-5-yl)benzyloxy]benzyl]imidazo-[4,5-b]pyridine and 4N hydrochloric acid in ethanol.
Yield: 75% of theory, Melting point: 198.degree.-200.degree. C. C.sub.29 H.sub.33 N.sub.9 O.sub.2 (539.64) Calculated: C 64.54 H 6.16 N 23.36 Found: 64.37 6.24 23.57
EXAMPLE 89
2-n-Butyl-6-(cyclohexylaminocarbonyl-N-methylamino)-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-6-(cyclohexylaminocarbonyl-N-methylamino)-1-[4-[(.alpha.-(N-triphenylmethyl)-tetrazol-5-yl)benzyloxy]benzyl]-benzimidazole and 4N hydrochloric acid in ethanol.
Yield: 85.7% of theory, Melting point: 195.degree.-197.degree. C. C.sub.34 H.sub.40 N.sub.8 O.sub.2 (592.74) Calculated: C 68.89 H 6.80 N 18.90 Found: 68.82 6.91 18.77
EXAMPLE 90
5,7-Dimethyl-2-ethyl-3-[4-[.alpha.-(1H-tetrazol-5-yl)-benzyloxy]-3,5-dichlorobenzyl]imidazo[4,5-b]pyridine hydrate
Prepared analogously to Example 57g from 5,7-dimethyl-2-ethyl-3-[4-[(.alpha.-(N-triphenylmethyl)tetrazol-5-yl)benzyloxy]-3,5-dichlorobenzyl]imidazo[4,5-b]pyridine and 85% formic acid in methylene chloride.
Yield: 77.2% of theory, Melting point: 143.degree.-145.degree. C. C.sub.25 H.sub.23 Cl.sub.2 N.sub.7 O.times.H.sub.2 O (526.42) Calculated: C 57.04 H 4.78 N 18.62 Cl 13.47 Found: 57.51 4.82 19.03 13.38
EXAMPLE 91
2-n-Butyl-6-cyclohexylaminocarbonylamino-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-3,5-dichlorobenzyl]-benzimidazole semihydrate
Prepared analogously to Example 57g from 2-n-butyl-6-cyclohexylaminocarbonylamino-1-[4-[(.alpha.-(N-triphenyl-methyl)tetrazol-5-yl)benzyloxy]-3,5-dichlorobenzyl]-benzimidazole and 85% formic acid in methylene chloride.
Yield: 83% of theory, Melting point: 129.degree.-131.degree. C. C.sub.33 H.sub.36 Cl.sub.2 N.sub.8 O.sub.2 .times.0.5 H.sub.2 O (655.62) Calculated: C 60.36 H 5.68 N 17.07 Found: 60.40 5.78 17.19
EXAMPLE 92
2-n-Butyl-6-dimethylaminocarbonylamino-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-3-methoxybenzyl]benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-6-dimethylaminocarbonylamino-1-[4-[(.alpha.-(N-triphenylmethyl)-tetrazol-5-yl)benzyloxy]-3-methoxybenzyl]benzimidazole and 4N hydrochloric acid in ethanol.
Yield: 75% of theory, Melting point: 158.degree.-162.degree. C. C.sub.30 H.sub.34 N.sub.8 O.sub.3 (554.65) Calculated: C 64.97 H 6.18 N 20.20 Found: 64.71 6.20 19.91
EXAMPLE 93
2-n-Propyl-4-methyl-6-(1-methyl-benzimidazol-2-yl)-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-3,5-dimethoxybenzyl]-benzimidazole hydrate
Prepared analogously to Example 57g from 2-n-propyl-4-methyl-6-(1-methyl-benzimidazol-2-yl)-1-[4-[(.alpha.-(N-triphenylmethyl)tetrazol-5-yl)benzyloxy]-3,5-dimethoxybenzyl]benzimidazole and 85% formic acid in methylene chloride.
Yield: 83% of theory, Melting point: 152.degree.-154.degree. C. C.sub.36 H.sub.36 N.sub.8 O.sub.3 .times.H.sub.2 O (646.75) Calculated: C 66.86 H 5.97 N 17.33 Found: 67.08 5.99 16.97
EXAMPLE 94
2-n-Propyl-4-methyl-6-(2,3-dimethylsuccinimino)-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-3,5-dimethoxybenzyl]-benzimidazole semihydrate
Prepared analogously to Example 57g from 2-n-propyl-4-methyl-6-(2,3-dimethylsuccinimino) -1-[4-[(.alpha.-(N-triphenylmethyl) tetrazol-5-yl)benzyloxy]-3,5-dimethoxybenzyl]benzimidazole and 85% formic acid in methylene chloride.
Yield: 74.6% of theory, Melting point: 192.degree.-144 .degree. C. C.sub.34 H.sub.37 N.sub.7 O.sub.5 .times.0.5 H.sub.2 O (632.71) Calculated: C 64.54 H 6.05 N 15.50 Found: 64.46 6.10 15.25
EXAMPLE 95
2-n-Propyl-4-methyl-6-(1-methyl-benzimidazol-2-yl)-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-3,5-dibromobenzyl]-benzimidazole
Prepared analogously to Example 57g from 2-n-propyl-4-methyl-6(1-methyl-benzimidazol-2-yl)-1-[4-[(.alpha.-(N-triphenylmethyl)tetrazol-5-yl)benzyloxy]-3,5dibromobenzyl]benzimidazole and 85% formic acid in methylene chloride.
Yield: 21.6% of theory, Melting point: 132.degree.-134.degree. C. C.sub.34 H.sub.30 Br.sub.2 N.sub.8 O (726.47) Calculated: C 56.21 H 4.16 N 15.42 Br 21.99 Found: 56.53 4.50 15.42 22.03
EXAMPLE 96
2-n-Butyl-5-dimethylaminocarbonylamino-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-3-methoxybenzyl]-benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-5-dimethylaminocarbonylamino-1-[4-[(.alpha.-(N-triphenylmethyl)-tetrazol-5-yl)benzyloxy]-3-methoxybenzyl]benzimidazole and 4N hydrochloric acid in ethanol.
Yield: 88.2% of theory, Melting point: 181.degree.-185.degree. C. C.sub.30 H.sub.34 N.sub.8 O.sub.3 (554.66) Calculated: C 64.96 H 6.18 N 20.20 Found: 65.18 5.92 19.97
EXAMPLE 97
5,7-Dimethyl-2-ethyl-3-[4-[.alpha.-(O-ethyl-phosphono)benzyloxy]benzyl]imidazo[4,5-b]pyridine sodium salt-semihydrate
Prepared analogously to Example 73b from 5,7-dimethyl-2-ethyl-3-3[4-[.alpha.-(O,O-diethylphosphono)benzyloxy]benzyl]imidazo[4,5-b]pyridine and potassium hydroxide in methanol.
Yield: 52% of theory, Melting point: from 150.degree. C. (decomp.) C.sub.26 H.sub.30 N.sub.4 NaO.sub.3 P.times.0.5 H.sub.2 O (509.52) Calculated: C 61.29 H 6.33 N 11.00 Found: 60.95 6.34 10.98
EXAMPLE 98
2-n-Propyl-4-methyl-1-[4-[.alpha.-(O-ethyl-phosphono)-N-methylbenzylamino]benzyl]benzimidazole semihydrate
a) 2-n-Propyl-4-methyl-1-[4-[.alpha.-(O,O-diethylphosphono)-N-methyl-benzylamino]benzyl]benzimidazole
1.1 g (2 mMol) of 2-n-propyl-4-methyl-1-[4-[.alpha.-(O,O-diethylphosphono)-benzylamino]benzyl]benzimidazole are dissolved in 1 ml of formic acid and mixed with 2 ml of a 37% formalin solution. The mixture is stirred for 2 hours at 100.degree. C., cooled and poured onto ice. After the addition of conc. ammonia the mixture is extracted twice with ethyl acetate. The combined organic phases are washed with saturated saline solution and dried over magnesium sulphate. The organic phase is evaporated down in vacuo and the residue obtained is purified over a silica gel column (particle size: 0.063-0.02 mm, ethyl acetate/petroleum ether=1:1 to 1:2). The uniform fractions are combined and evaporated down in vacuo.
Yield: 0.60 g (58% of theory), R.sub.f value: 0.45 (silica gel; eluant: petroleum ether/ethyl acetate=1:1)
b) 2-n-Propyl-4-methyl-1-[4-[.alpha.-(O-ethyl-phosphono)-N-methyl-benzylamino]benzyl]benzimidazole semihydrate
Prepared analogously to Example 73b from 2-n-propyl-4-methyl-1-[4-[.alpha.-(O,O-diethylphosphono)-N-methylbenzylamino]benzyl]benzimidazole and potassium hydroxide in methanol.
Yield: 77% of theory, Melting point: from 106.degree. C. (decomp.) C.sub.28 H.sub.34 N.sub.3 O.sub.3 P.times.0.5 H.sub.2 O (500.59) Calculated: C 67.18 H 7.05 N 8.40 Found: 67.25 7.02 8.42
EXAMPLE 99
2-n-Propyl-4-methyl-1-[4-[.alpha.-(O-ethyl-phosphono)benzylamino]benzyl]benzimidazole hydrobromide
0.5 g (1.0 mMol) of 2-n-propyl-4-methyl-1-[4-[.alpha.-(O,O-diethylphosphono)benzylamino]benzyl]benzimidazole are dissolved in 10 ml of 48% hydrobromic acid and refluxed for 5 hours. After cooling at ambient temperature, the pH is adjusted to 6 by the addition of concentrated ammonia and glacial acetic acid. The precipitate formed is suction filtered and dried over potassium hydroxide at 40.degree. C.
Yield: 47% of theory, Melting point: from 140.degree. C. (decomp.) C.sub.25 H.sub.28 N.sub.3 O.sub.3 P.times.HBr (530.42) Calculated: C 56.61 H 5.51 N 7.92 Found: 56.48 5.80 8.05
EXAMPLE 100
2-n-Propyl-4-methyl-1-[4-[(.alpha.-methanesulphonylaminocarbonyl)benzyloxy]benzyl]benzimidazole
410 mg (1 mMol) of 2-n-propyl-4-methyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole are dissolved in 100 ml of thionylchloride, mixed with 1 drop of dimethylformamide and refluxed for 1 hour. Then, after the addition of toluene, the mixture is evaporated to dryness and taken up in 30 ml of acetone. After the addition of 1 ml of triethylamine and 150 mg (1.58 mMol) of methanesulphonic acid amide the mixture is stirred for 2 hours at ambient temperature. The reaction mixture is then evaporated down and the residue is purified over a silica gel column (particle size: 0.063-0.02 mm; methylene chloride/ethanol=50:1, 19:1, 9:1 and 4:1). The uniform fractions are combined, evaporated down and the residue obtained is triturated with ether and dried.
Yield: 15.3% of theory, Melting point: 139.degree.-141.degree. C. C.sub.27 N.sub.29 N.sub.3 O.sub.4 S (491.60) Calculated: C 65.95 H 5.94 N 8.54 Found: 65.67 6.27 8.89
EXAMPLE 101
2-Ethyl-4-methyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-(n-butanesultam-1-yl)benzimidazole
Prepared analogously to Example 1b from 2-ethyl-4-methyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-(n-butanesultam-1-yl)benzimidazole and 2N sodium hydroxide solution in ethanol.
EXAMPLE 102
2-Ethyl-4-methyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-benzyl]-6-(n-butanesultam-1-yl)benzimidazole
Prepared analogously to Example 57g from 2-ethyl-4-methyl-1-[4-[(.alpha.-(N-triphenylmethyl)tetrazol-5-yl)-benzyloxy]benzyl]-6-(n-butanesultam-1-yl)-benzimidazole and 4N hydrochloric acid in ethanol.
EXAMPLE 103
2-n-Butyl-1-[4-[.alpha.-(ethoxycarbonyl)-.alpha.-(2-pyridyl)-methoxy]benzyl]-6-(N-cyclohexylaminocarbonyl-methylamino)-benzimidazole
Prepared analogously to Example 1a from 2-n-butyl-1-(4-hydroxybenzyl)-6-(N-cyclohexylaminocarbonyl-methylamino)benzimidazole and ethyl .alpha.-bromo-pyridyl-2-acetate.
Yield: 90.0% of theory, R.sub.f value: 0.70 (silica gel; ethyl acetate/ethanol/ammonia=90:10:1) C.sub.33 H.sub.44 N.sub.5 O.sub.4 (574.76) Calculated: C 70.20 H 7.40 N 11.69 Found: 69.97 7.26 11.21
EXAMPLE 104
2-n-Butyl-3-[4-[(.alpha.-carboxy)benzyloxy]-3-methoxybenzyl]-5-methyl-6-dimethylaminocarbonylamino-imidazo[4,5-b]-pyridine
Prepared analogously to Example 1b from 2-n-butyl-3-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-3-methoxybenzyl]-5-methyl-6-dimethylaminocarbonylamino-imidazo[4,5-b]pyridine and 2N sodium hydroxide solution in ethanol.
Yield: 91.8% of theory, Melting point: 174.degree.-176.degree. C. R.sub.f value: 0.60 (silica gel; ethyl acetate/ethanol/ammonia=50:45:5) C.sub.30 H.sub.35 N.sub.5 O.sub.5 (545.65) Calculated: C 66.04 H 6.47 N 12.84 Found: 66.25 6.39 12.95
EXAMPLE 105
2-n-Butyl-1-[4-[.alpha.-(ethoxycarbonyl)-.alpha.-(2-pyridyl)meth-oxy]benzyl]-6-dimethylaminocarbonylamino-benzimidazole
Prepared analogously to Example 1a from 2-n-butyl-1-(4-hydroxybenzyl)-6-dimethylaminocarbonylamino-benzimidazole and ethyl .alpha.-bromo-pyridyl-2-acetate.
Yield: 87.5% of theory, R.sub.f value: 0.50 (silica gel; ethyl acetate/ethanol/ammonia=90:10:1) C.sub.30 H.sub.35 N.sub.5 O.sub.4 (529.65) Calculated: C 68.03 H 6.66 N 13.22 Found: 68.09 6.83 13.06
EXAMPLE 106
2-n-Butyl-1-[4-[.alpha.-(ethoxycarbonyl)-.alpha.-(2-pyridyl)meth-oxy]benzyl]-6-cyclohexylcarbonylamino-benzimidazole
Prepared analogously to Example 1a from 2-n-butyl-1-(4-hydroxybenzyl)-6-cyclohexylcarbonylamino-benzimidazole and ethyl .alpha.-bromopyridyl-2-acetate.
Yield: 75.3% of theory, R.sub.f value: 0.50 (silica gel; ethyl acetate/ethanol/ammonia=90:10:1) C.sub.34 H.sub.40 N.sub.4 O.sub.4 (568.73) Calculated: C 71.80 H 7.09 N 9.35 Found: 71.82 7.2 1 9.83
EXAMPLE 107
2-n-Butyl-3-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-3-methoxybenzyl]-5-methyl-6-dimethylaminocarbonylamino-imidazo[4,5-b]pyridine
Prepared analogously to Example 57g from 2-n-butyl-3-[4-[.alpha.-((N-triphenylmethyl)-tetrazol-5-yl)benzyloxy]-3-methoxybenzyl]-5-methyl-6-dimethylaminocarbonylamino-imidazo[4,5-b]pyridine and 4N hydrochloric acid in ethanol.
Yield: 60.0% of theory, Melting point: 197.degree.-199.degree. C. R.sub.f value: 0.60 (silica gel; ethyl acetate/ethanol/ammonia=50:45:5) C.sub.30 H.sub.35 N.sub.9 O.sub.3 (569.68) Calculated: C 63.25 H 6.19 N 22.13 Found: 62.94 6.13 21.87
EXAMPLE 108
2-n-Butyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)-.alpha.-(2-pyridyl)methoxy]benzyl]-6-dimethylaminocarbonylamino-benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-1-[4-[.alpha.-((N-triphenylmethyl)-tetrazol-5-yl)-.alpha.-(2-pyridyl)methoxy]benzyl]-6-dimethylaminocarbonylamino-benzimidazole and 4N hydrochloric acid in ethanol.
Yield: 25.0% of theory, Melting point: 197.degree.-203.degree. C. R.sub.f value: 0.50 (silica gel; ethyl acetate/ethanol/ammonia=50:45:5) C.sub.28 H.sub.31 N.sub.9 O.sub.2 (525.63) Mass spectrum: (M+H)=526
EXAMPLE 109
2-Ethyl-3-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]-5-methyl-6-dimethylaminocarbonylamino-imidazo[4,5-b]-pyridine
Prepared analogously to Example 57g from 2-ethyl-3-[4-[.alpha.-((N-triphenylmethyl)-tetrazol-5-yl)benzyloxy]benzyl]-5-methyl-6-dimethylaminocarbonylamino-imidazo[4,5-b]-pyridine and 4N hydrochloric acid in ethanol.
Yield: 50.0% of theory, Melting point: 234.degree.-236.degree. C. C.sub.27 H.sub.29 N.sub.9 O.sub.2 (511.60) Calculated: C 63.39 H 5.71 N 24.64 Found: 63.36 5.87 24.52
EXAMPLE 110
2-n-Butyl-4-methyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-benzyl]-6-(2,3-dimethylsuccinimino)benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-4-methyl-1-[4-[.alpha.-((N-triphenylmethyl)-tetrazol-5-yl)-benzyloxy]benzyl]-6-(2,3-dimethylsuccinimino)-benzimidazole and 4N hydrochloric acid in ethanol.
Yield: 80.0% of theory, Melting point: 175.degree.-177.degree. C. C.sub.33 H.sub.35 N.sub.7 O.sub.3 (577.70) Calculated: C 68.61 H 6.11 N 16.97 Found: 68.45 6.24 17.00
EXAMPLE 111
2-n-Butyl-4-methyl-1-[4-[(.alpha.-carboxy)benzyloxy]benzyl]-6-cyclohexylcarbonylamino-benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-4-methyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-cyclohexylcarbonylaminobenzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 81.8% of theory, Melting point: 191.degree.-193.degree. C. C.sub.34 H.sub.39 N.sub.3 O.sub.4 (553.70) Calculated: C 73.75 H 7.10 N 7.58 Found: 73.70 6.94 7.48
EXAMPLE 112
2-n-Butyl-4-methyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]-benzyl]-6-cyclohexylcarbonylamino-benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-4-methyl-1-[4-[.alpha.-((N-triphenylmethyl)-tetrazol-5-yl)-benzyloxy]benzyl]-6-cyclohexylcarbonylamino-benzimidazole and 4N hydrochloric acid in ethanol.
Yield: 85.0% of theory, Melting point: 195.degree.-198.degree. C. C.sub.34 H.sub.39 N.sub.7 O.sub.2 (577.72) Calculated: C 70.68 H 6.80 N 16.97 Found: 70.81 6.98 16.97
EXAMPLE 113
2-n-Butyl-4-methyl-1-[4-[.alpha.-(carboxy)benzyloxy]benzyl]-6-tert.butylcarbonylamino-benzimidazole
Prepared analogously to Example 1b from 2-n-butyl-4-methyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]-6-tert.butylcarbonylamino-benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: 78.6% of theory, Melting point: 179.degree.-181.degree. C. C.sub.32 H.sub.37 N.sub.3 O.sub.4 (527.67) Calculated: C 72.34 H 7.07 N 7.96 Found: 72.51 6.95 7.65
EXAMPLE 114
2-n-Butyl-4-methyl-1-[4-[.alpha.-(tetrazol-5-yl)benzyloxy]-benzyl]-6-tert.butylcarbonylamino-benzimidazole
Prepared analogously to Example 57g from 2-n-butyl-4-methyl-1-[4-[.varies.-((N-triphenylmethyl)-tetrazol-5-yl)benzyloxy]benzyl]-6-tert.butylcarbonylamino-benzimidazole and 4N hydrochloric acid in ethanol.
Yield: 75.0% of theory, Melting point: 191.degree.-193.degree. C. C.sub.32 H.sub.37 N.sub.7 O.sub.2 (551.70) Calculated: C 69.67 H 7.76 N 17.77 Found: 69.42 7.80 17.68
EXAMPLE 115
2-Ethoxy-7-ethoxycarbonyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 63a from 2-ethoxy-7-ethoxycarbonyl-1-(4-hydroxy)benzyl-benzimidazole and ethyl 2-bromo-phenylacetate.
Yield: % of theory, Melting point: -.degree.C. R.sub.f value: 0. (silica gel); C.sub.29 H.sub.30 N.sub.2 O.sub.6 (488.57) Calculated: C H N Found:
EXAMPLE 116
2-Ethoxy-7-carboxy-1-[4-(.alpha.-carboxy)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-ethoxy-7-ethoxycarbonyl-1-[4-[(.alpha.-ethoxycarbonyl)benzyloxy]-benzyl]benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: % of theory, Melting point: -.degree.C. R.sub.f value: 0. (silica gel;) C.sub.25 H.sub.22 N.sub.2 O.sub.6 (446.46) Calculated: C H N Found:
EXAMPLE 117
2-Ethoxy-7-ethoxycarbonyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 57g from 2-ethoxy-7-ethoxycarbonyl-1-[4-[.alpha.-((N-triphenylmethyl)tetrazol-5-yl)benzyloxy]benzyl]benzimidazole and methanolic hydrochloric acid.
Yield: % of theory, Melting point: -.degree.C. R.sub.f value: 0. (silica gel;) C.sub.27 H.sub.26 N.sub.6 O.sub.4 (498.55) Calculated: C H N Found:
EXAMPLE 118
2-Ethoxy-7-carboxy-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole
Prepared analogously to Example 1b from 2-ethoxy-7-ethoxycarbonyl-1-[4-[.alpha.-(1H-tetrazol-5-yl)benzyloxy]benzyl]benzimidazole and 2N sodium hydroxide solution in ethanol.
Yield: % of theory, Melting point: -.degree.C. R.sub.f value: 0. (silica gel;) C.sub.25 H.sub.22 N.sub.6 O.sub.4 (470.49) Calculated: C H N Found:
In the Examples of pharmaceutical Formulations which follow, any suitable compound of formula I, particularly compounds A to I of the pharmacological test report, may be used as the active substance:
EXAMPLE I
Ampoules containing 50 mg of active substance per 5 ml
______________________________________Active substance 50 mgKH.sub.2 PO.sub.4 2 mgNa.sub.2 HPO.sub.4 .times. 2H.sub.2 O 50 mgNaCl 12 mgWater for injections ad 5 ml______________________________________
Preparation:
The buffer substances and isotonic substance are dissolved in some of the water. The active substance is added and, once it has been completely dissolved, water is added to make up the required volume.
EXAMPLE II
Ampoules containing 100 mg of active substance per 5 ml
______________________________________Active substance 100 mgMethyl glucamine 35 mgGlycofurol 1000 mgPolyethyleneglycol-polypropylene- 250 mgglycol block polymerWater for injections ad 5 ml______________________________________
Preparation:
Methyl glucamine is dissolved in some of the water and the active substance is dissolved with stirring and heating. After the addition of solvents, water is added to make up the desired volume.
EXAMPLE III
Tablets containing 50 mg of active substance
______________________________________Active substance 50.0 mgCalcium phosphate 70.0 mgLactose 40.0 mgCorn starch 35.0 mgPolyvinylpyrrolidone 3.5 mgMagnesium stearate 1.5 mg 200.0 mg______________________________________
Preparation:
The active substance, CaHPO.sub.4, lactose and corn starch are uniformly moistened with an aqueous PVP solution. The mass is passed through a 2 mm screen, dried at 50.degree. C. in a circulating air dryer and screened again.
After the lubricant has been added, the granules are compressed in a tablet making machine.
EXAMPLE IV
Coated tablets containing 50 mg of active substance
______________________________________Active substance 50.0 mgLysine 25.0 mgLactose 60.0 mgCorn starch 34.0 mgGelatin 10.0 mgMagnesium stearate 1.0 mg 180.0 mg______________________________________
Preparation:
The active substance is mixed with the excipients and moistened with an aqueous gelatin solution. After screening and drying the granules are mixed with magnesium stearate and compressed to form tablet cores.
The cores thus produced are covered with a coating by known methods. A colouring may be added to the coating suspension or solution.
EXAMPLE V
Coated tablets containing 100 mg of active substance
______________________________________Active substance 100.0 mgLysine 50.0 mgLactose 86.0 mgCorn starch 50.0 mgPolyvinylpyrrolidone 2.8 mgMicrocrystalline cellulose 60.0 mgMagnesium stearate 1.2 mg 350.0 mg______________________________________
Preparation:
The active substance is mixed with the excipients and moistened with an aqueous PVP solution. The moist mass is passed through a 1.5 mm screen and dried at 45.degree. C. After drying, it is screened again and the magnesium stearate is added. This mixture is compressed into cores.
The cores thus produced are covered with a coating by known methods. Colourings may be added to the coating suspension or solution.
EXAMPLE VI
Capsules containing 250 mg of active substance
______________________________________Active substance 250.0 mgCorn starch 68.5 mgMagnesium stearate 1.5 mg 320.0 mg______________________________________
Preparation:
The active substance and corn starch are mixed together and moistened with water. The moist mass is screened and dried. The dry granules are screened and mixed with magnesium stearate. The final mixture is packed into size 1 hard gelatine capsules.
EXAMPLE VII
Oral suspension containing 50 mg of active substance per 5 ml
______________________________________Active substance 50.0 mgHydroxyethylcellulose 50.0 mgSorbic acid 5.0 mg70% sorbitol 600.0 mgGlycerol 200.0 mgFlavouring 15.0 mgWater ad 5.0 ml______________________________________
Preparation:
Distilled water is heated to 70.degree. C. Hydroxyethyl-cellulose is dissolved therein with stirring. By the addition of sorbitol solution and glycerol the mixture is cooled to ambient temperature. At ambient temperature, sorbic acid, flavouring and active substance are added. The suspension is evacuated with stirring to remove any air. One dose of 50 mg is contained in 5.0 ml.
EXAMPLE VIII
Suppositories containing 100 mg of active substance
______________________________________Active substance 100.0 mgSolid fat 1600.0 mg(adeps solidus) 1700.0 mg______________________________________
Preparation:
The hard fat is melted. At 40.degree. C. the ground active substance is homogeneously dispersed in the melt. It is cooled to 38.degree. C. and poured into slightly chilled suppository moulds.

Number	Name	Date
4439226	Pilgram	Mar 1984
4714762	Hoefle et al.	Dec 1987
4880804	Carini et al.	Nov 1989
5030643	Bernstein et al.	Jul 1991
5084575	Kreft et al.	Jan 1992
5116860	Buerstinghaus et al.	May 1992
5128359	Bru-Magniez et al.	Jul 1992
5171748	Roberts et al.	Dec 1992
5216003	Vaquez	Jun 1993
5314880	Whittaker et al.	May 1994
5389634	Fortin et al.	Feb 1995

Number	Date	Country
0360098	Mar 1990	EPX
0385850	Sep 1990	EPX
4142366	Jun 1993	DEX

Phenylalkyl derivatives, with pharmaceutical activity

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