Photodynamic cosmetic procedure and healing method

Abstract

Innovative non-surgical methods and compositions are provided for administering PDT to promote tissue regeneration or augmentation while minimizing scarring and risk of infection. Among several areas of application, is the treatment of acute and chronic wounds which have afflicted epidermal and connective tissue layers of the body. Another application area is as cosmetic surgery/treatments, including: reducing wrinkles, sulcus, scars (acne or traumatic caused), sequelae cellulite, as well as for other skin irregularities, to give a smoother skin surface. This invention consists of a collagen based or other suitable biodegradable supporting matrix which is embedded with a liposomal loaded photosensitizer. In one embodiment of this invention a liposomal formulated photosensitizer is first injected at the site followed by collagen implantation and PDT treatment. In another embodiment a liposomal formulated photosensitizer is incorporated in the collagen. Generally 30 minutes after collagen is mixed with photosensitizer the light activation is done. The matrix may also carry important growth factors and cytokines, which promote fibroblast cell migration and proliferation, to the wound site. Microbial infection at the wound site can also be controlled by antibacterial PDT.

Description

BRIEF DESCRIPTION OF FIGURES

FIG. 1 shows biopsy results 14 days after treatment in mice.

FIG. 2 macroscopic picture of thermic stability of collagen during laser irradiation.

FIG. 3 the thermic stability of collagen when exposed to different energy levels at microscopic level.

Claims

1. A method of tissue reformation and/or augmentation consisting of the steps: a) administrating a ‘photosensitizer’ to an area to be treated;b) administrating a suitable, compatible tissue matrix or substitute composition;c) waiting a sufficient time for said photosensitizer loaded matrix to reach an effective concentration in said area of said treated area; and,d) activating said photosensitizer loaded matrix with electromagnetic radiation of a wavelength absorbed by said ‘photosensitizer’ to the treated area.

2. The method according to claim 1, wherein steps a) and b) can be done simultaneously, or separately in either order.

3. The method according to claim 1, wherein said photosensitizer loaded matrix is directed at the treatment of superficial skin wounds, veins/arteries, organs, and bones.

4. The method according to claim 3, wherein said photosensitizer loaded matrix comprises a collagen mixture.

5. The method according to claim 1, further including the step of applying a biodegradable matrix to said treated area.

6. The method according to claim 1, further comprising the step of mixing a liposomal formulation of temoporfin in a carrier for said ‘photosensitizer’.

7. The method according to claim 6, wherein said concentration of said temoporfin is at least 0.001 mg/ml.

8. The method according to claim 6, further including cellular material from an area nearby said treatment area.

9. The method according to claim 8, wherein said cellular material is adipose tissue.

10. The method for treating wounds for improved healing according to claim 1, wherein said electromagnetic radiation is laser radiation.

11. A biocompatible composition suitable for use in tissue regeneration and wound healing comprising: at least one biocompatible matrix material, and a photosensitizer.

12. The composition according to claim 11, wherein said biocompatible matrix material is a biodegradable matrix material.

13. The composition according to claim 12, wherein said biodegradable matrix material is comprised of a material selected from the group consisting of: a hydrogel, a polymer, a glycoprotein, a collagen, an adipose, a non-animal stabilized hyaluronic acid, a polysaccharide, a polypeptide, and combinations of these materials.

14. The composition according to claim 11, wherein said composition includes at least one cellular component selected from the group consisting of epidermal growth factor, platelet derived growth factor, tissue angiogenesis growth factor, cytokines, keratinocytes, epidermal cells, and fibroblasts.

15. The composition according to claim 11, wherein said photosensitizer is a derivative with a parent structure selected from the group consisting of: porphyrin, porphyrinogen, hematoporphyrin, pheophorbide, dihydro- and tetrahydro-tetrapyrroles.

16. The composition according to claim 15, wherein said photosensitizer is encapsulated in liposomes.

17. The composition according to claim 15, wherein said photosensitizer is pegylated.

18. The composition according to claim 16, wherein said liposomes are pegylated.