Patent Application
20070099240
Pursuant to 37 C.F.R. § 1.52(e)(1)(iii), a compact disc containing an electronic version of the Sequence Listing has been submitted concomitant with this application, the contents of which are hereby incorporated by reference. A second compact disc is submitted and is an identical copy of the first compact disc. The discs are labeled “copy 1” and “copy 2,” respectively, and each disc contains one file entitled “2183-6064.1US SEQ LIST.txt” which is 82 KB and created on Aug. 14, 2003.
1. Field of the Invention
The invention relates to the detection of (bio)molecules, or analogues thereof, in micro-arrays and the supports used for the micro-arrays. More particularly, the invention relates to methods for determining or testing binding of a first member molecule within an array or a library of tentative first member binding molecules with a second member binding molecule, wherein the first and second molecules are each members of a binding pair. The invention further relates to enzyme-linked detection of the binding pair in high-density micro-array systems.
2. State of the Art
Interactions, or the formation of a specific binding pair, between binding molecules, which in general are bio-molecules, and their corresponding ligands, which in general are also bio-molecules, are central to life. Cells often bear or contain receptor molecules that interact or bind with a hormone, a peptide, a drug, an antigen, an effector molecule or with another receptor molecule. Examples of binding pairs include: enzymes that bind with their respective substrate; antibody molecules that bind with an antigen; nucleic acids that bind with a protein, and so on. The terms “interact” or “bind” as used herein are meant to refer to the range of molecular forces between the binding molecule and ligand (or the functional parts thereof) as they approach each other, and may influence each other's properties. This approach takes the binding molecule and its ligand through various stages of molecular recognition comprising increasing degrees of intimacy and mutual effect, thus the two members bind and form a pair.
Binding molecules have this binding ability because the binding molecules have distinct binding sites that allow for the recognition of the ligand in question. The ligand, in turn, has a corresponding binding site, and when the two binding sites interact—essentially spatial—complementarity, the two molecules can bind. Molecules typically have three dimensions and binding sites are often of a three dimensional nature, wherein one molecule includes one or more surface projections or protuberances as one binding site that corresponds to one or more pockets or depressions on the other binding molecule, such as a three-dimensional lock-and-key arrangement that may be an induced-fit variety.
Due to the central role binding molecules and their ligands play in life, there is an ever expanding interest in testing for or identifying the nature or characteristics of the binding site and the members of the binding pair of molecules involved in such a site. Not only is one interested in the exact nature of the particular interaction between the binding molecule and ligand in question, e.g., in order to replace or supplement binding molecules or ligands when needed, one is also interested in knowing the approximating characteristics of the interaction in order to find, or design, analogs, agonists, antagonists or other compounds that mimic a binding site or ligand involved.
Versatile and rapid methods to test for or identify binding pairs and its separate members exist. For instance, most, if not all nucleic acid detection techniques, and molecular libraries using these detection techniques entail hybridization of an essentially continuous nucleic acid stretch with a complementary nucleic acid strand, such as DNA, RNA or PNA. Proteins and peptides are often detected using antibodies, or derivatives or synthetic variants thereof. Arrays of biological molecules, i.e., micro-arrays, are used in standard techniques in many laboratories. Such micro-array-based detection generally includes a method in which a member of a specific binding pair is detected by means of an optically detectable reaction. Different supports for the libraries including tentative or possible first members of the binding pair (such as nucleic acid, peptide, or of any other nature) are used, but can be divided into two types: porous surfaces and non-porous surfaces.
Porous surfaces, such as membranes, cellulose and paper, are probably the oldest support in use. For example, “dot blots” are widely used today. The synthesis of macromolecules, e.g., nucleic acids or peptides, has been described on these porous matrices. Paper was used as a relatively thick, continuous porous matrix on which first member constructs were synthesized spot wise. Binding pairs were generally identified by detection with, directly or via indirectly, enzyme labeled probes, thus, allowing increased sensitivity over the use of probes that were directly labeled with an optically detectable reporter molecule, such as a fluorescent group. A disadvantage of these methods is that the density of spots in these matrices is limited. This limitation is caused, inter alia, by the diffusion of the enzymatically changed substrate in the matrix. To avoid the diffusion and, thus, the inaccurate localization of a binding pair in the field of peptide synthesis, methods using polyethylene pins (Geysen, 1983) or in polypropylene wells (Slootstra, 1995; 1997) exist. However, these “early” methods have the disadvantage that no high density arrays (approximately up to not more than 10-20 spots/cm2) can be facilitated for various reasons.
The existence of limited spot densities in the arrays is the reason why more nonporous surfaces are becoming more widely used. In the field of genomics, huge arrays of polynucleotide sequences are spotted on a variety of surfaces, typically glass slides covered with different coatings (See, U.S. Pat. Nos. 6,015,880 and 5,700,637). Array densities of 1000 spots/cm2 are possible. Even higher densities are possible when the biomolecules (i.e., polynucleotide sequences) are synthesized in-situ (See, U.S. Pat. No. 5,871,928). For example, in a traditional gene expression assay designed to profile the expression of many genes in parallel, mRNA is prepared from two different tissue types, e.g., normal and diseased samples. The isolated mRNA represents a snapshot of the current state of expression within the cells. The mRNA is converted to DNA via a first strand cDNA labeling reaction. After target DNA is deposited onto coated glass slides and directly labeled cDNA probes are hybridized to the arrays, the hybridized arrays are imaged using an array scanner and the results are examined for differences in expression levels using several image and data analysis software tools. A more intricate porous support surface has been described for these purposes (See, PCT International Publication WO 00/56934) that uses continuous porous matrix arrays. On microscope slides, a continuous slab of polyacrylamide is formned, e.g., 20 um thick and having a thin, continuous porous matrix (hydrogel) that is combined with a non porous surface (glass).
Detection of specific binding pairs on or in the high-density supports is achieved with directly labeled probes that include optically detectable, e.g., fluorescent, nucleotides or antibodies. The detection techniques are highly sensitive, have low non-specific binding and high photo stability. Labeled nucleotides are widely used for labeling DNA and RNA probes, especially for multicolor analysis in micro-arrays, but also for FISH, chromosome identification, whole chromosome painting, karyotyping and gene mapping.
Labeled nucleotides are available in a range of bright, intense colors having narrow emission bands that are ideal for multiplexing within a single sample. For protein or peptide detection, fewer fluorescently labeled probes are available since the field of protein or peptide based high-density micro-array systems is not as well developed as micro-arrays based on nucleic acid detection.
In one exemplary embodiment, an array or library of first members is disclosed, in general spatially and/or addressable bound, most often covalently, to such a support, e.g., by spotting or gridding. A second member, the detecting or specific binding molecule, which is directly or indirectly labeled with a marker molecule (such as a fluorescent compound) to facilitate optical detection of the aforementioned pair is also disclosed to detect the putative first members of the array with which it can bind. The second member can of course be a nucleic acid, a receptor molecule, an antibody or the like. Binding of the second member, thus, identifies the first member because of its specific localization on the support.
The invention combines the advantages of high density arraying, i.e., testing a lot of binding events at once, and enzyme-linked assays (very sensitive), thus, allowing for the detection of more binding pairs more rapidly. Micro-array systems are disclosed herein that work with enzyme-linked assays in order to detect the molecule of interest on a high-density support. Such testing of high densities of constructs on a solid support in a enzyme-linked assay is disclosed by the invention, wherein a first member is attached to or synthesized on one side of a surface of the support in a density of at least 25, at least 50, at least 100, about 200-500, or even 1000 spots per square centimeter.
In one exemplary embodiment, the invention describes a support of polymeric material, i.e., a polymeric support, having a library of spots of the tentative first member binding molecules in a density of at least 25 spots, at least 50 spots, at least 100 spots, about 200-500 spots, or even 1000 spots per square centimeter. In another exemplary embodiment, the polymeric material comprises polypropylene having hydrophilic patches.
The first binding pair members may be spotted or gridded in a positionally or spatially addressable way such that many different constructs or first member molecules on the support can interact with a second member or binding molecule. The spots can overlap, as long as the constituting collection of first member molecules are spatially addressable and distinct. Spotting can be done using piezo drop-on-demand technology or by using miniature solenoid valves. Gridding can be done using a set of individual needles that pick up sub-microliter amounts of segment solution from a microtiter plate, wherein the segment solution includes solutions having the first members.
When peptides are tested, the support may be de-protected and extensively washed after the linking reaction to remove uncoupled peptides. The disclosed method gives a peptide construct density as large as 25 to 50, even 100 to 200, or up to 500 to 1000 spots per square centimeter. This density allows for the screening of a large number of possible peptide constructs of the proteins that bind with an antibody. For example, in one exemplary embodiment, 25,000 to 100,000 constructs are made on a 1000 square centimeter surface. The surface is screened for binding in an enzyme-linked assay, be it directly or indirectly, wherein a fluorescent substrate is generated with 100 ml of enzyme-labeled probe solution that contains 1-10 μg of probe/ml. The screened surface is developed with an optically detectable substrate using established techniques.
The invention, thus, discloses a method for determining the binding of a first member molecule from a library of tentative first member binding molecules with a second member binding molecule. The method comprises providing a polymeric (such as the polypropylene type surface having hydrophilic patches) or plastic support with a library of spots of the tentative first member binding molecules in a density of at least 25 spots per square centimeter. The method further comprises detecting the binding of the first member molecule with the second member molecule with an enzyme-linked assay, wherein the enzyme-linked-assay comprises the production of a fluorescent or chemiluminescent substrate. Fluorescent substrates can be produced with a host of enzyme systems, such as horse-radish-peroxidase, alkaline phosphatase or other known substrate-enzyme systems such as those disclosed in Mendoza et al. including “High-throughput microarray-based enzyme-linked immunosorbent assay (ELISA),” Biotechniques, Eaton Publishing, Natick, US vol. 27, (1999), where an optically flat, glass-based support is described, including multitudes of identically patterned arrays of antigens printed to the glass.
As described herein, indirect or direct fluorescence detection allocates antibody binding constructs. For instance, direct fluorescence detection with confocal scanning detection methods allows antibody detection on spots generated with droplets having peptide-solution in the sub-nanoliter range, which makes even higher construct densities feasible. Nucleic acid libraries may be made in a similar fashion using enzyme-labeled nucleic acid probes.
Furthermore, the invention describes a support for a micro-array configured for testing binding of a first member molecule within an array or library of tentative first member binding molecules with a second member binding molecule. The support includes a surface having patches that are interspersed within areas, wherein the areas are materially distinct from the patches. In general, the surface is obtained using various complicated methods that comprise masking and subsequent photographic exposure and development (See, PCT International Publication WO 94/27719), plasma treatment, polymerization, photo-oxidation or electron beam treatment (See, WO 99/58245). Other techniques (See, Canadian Publication CA 2260807) require an inert solid support material to which the hydrophobic and hydrophilic areas need be applied, such as by way of coating. Other techniques (See, Great Britain Publication GB 2332273) provide an extremely hydrophobic surface in relation to the sample solution that is applied after which samples are thought to adhere to the surface by drying. In U.S. Pat. No. 5,369,102, a support with two opposing surfaces, one hydrophobic and the opposing one hydrophilic, are provided for the attachment of cells to the hydrophilic surface. PCT International Publication WO 99/32705 discloses various grafting protocols, but does not disclose requirements as to roughness of the surface, or to a pattern of hydrophilic and hydrophobic patches.
The present invention has recognized that masking or coating is not required and that grafting surfaces, such as polypropylene, is suitable provided that at least one side of the surface of the starting material, such as a substantially flat surface of at least 0.5 square centimeters or at least 1 square centimeter, is configured with a substantial roughness characterized by elevations and depressions. The rough surface allows for the interspersed character of hydrophobic and hydrophilic patches to occur on the side or surface. The pattern of hydrophilic (hydrophilic matrices typically cause severe diffusion) and hydrophobic areas (which may block diffusion) as disclosed herein diminish diffusion, especially when the patches are smaller than the droplet size of dispensed material (spots), which are the smallest when the spot density is the highest.
In one exemplary embodiment, the invention discloses a support, wherein the surface of the areas essentially comprises relatively hydrophobic polypropylene and the surface of the patches essentially comprise polypropylene having a relatively hydrophilic material, such a grafted polyacrylic acid. The support disclosed herein comprises at least a spot or dot, e.g. a collection of first member molecules such as a nucleic acid or peptide construct, density as large as 25, or up to 50, 100, 200, 500 or even 1,000 spots per square centimeter. Further, the spots or dots are positionally or spatially addressable, wherein each of the spot or dot covers at least one patch, but the spot or dot may cover from 3-5, or even from 5-15 or more hydrophilic patches or pixels.
Although the surface, such as the polypropylene, may not be completely covered with a homogenous graft, the high loadings of peptide or nucleotide per square centimeter are possible due to the relatively high surface occupation of the grafts, such as polyacrylic acid, on the surface. Thicker grafts can carry higher peptide or nucleic acid loadings, but may suffer from more diffusion problems of dispensed material because of the growing occupation of grafted surface. Thus, the material can be made to suit various needs as regard to loading versus diffusion.
The invention further discloses a solid support having at least one peptide, or at least one nucleotide. The solid support may include a plurality of peptides (or likewise of nucleotides), wherein the peptides or nucleotides are arranged in spots.
In another exemplary embodiment, the invention describes a method for determining binding of a first member molecule within a library of tentative first member binding molecules with a second member binding molecule. The method includes providing at least one surface of a support having a library of spots of the tentative first member binding molecules, detecting the binding in an enzyme-linked assay and providing for limited, minimalized or restricted diffusion of an optically detectable marker molecule. Since diffusion is limited, the enzymatic reaction and the deposit or localization of the resulting (optically) detectable marker molecules can be determined with more precision and allow for higher densities than with previous micro-arrays using enzyme-linked-detection.
In a further exemplary embodiment, the invention discloses a method where the diffusion is limited by providing at least one surface of the support having surface patches that are interspersed within surface areas, wherein the surface areas are materially distinct from the patches. (See,
The patches and surrounding areas may be in a strict matrix or grid format, but this is not necessary. The patches are at least one or two dimensions smaller than the size of the circumference of the positioned droplets or spots of first member molecules that, in a later phase, will be provided to the support surface. Since the patches are smaller than the droplets or spots, at least 3-5 or at least 10-20 hydrophilic patches will fit within the circumference of a later spotted spot or droplet of the solution of a first member, whether the first member is a nucleic acid, a peptide, any other (bio-)molecule or combination thereof. The patches resemble pixels that, after a marker molecule has attached to a specific binding pair, create the optically detectable image, wherein a spot with a collection of first member molecules bound to second member molecules is detected.
In another exemplary embodiment, a one-to-one fit of pixel or patch to droplet or spot is also feasible even when the patch is larger than a spot, but this not necessary. It is also not necessary to apply or provide the patches in a regular pattern. When a droplet or spot is provided, the interspersed hydrophobic character of the support surface will limit the diffusion of any aqueous solution. The diffusion of a solution of an optically detectable substrate (be it as precipitate or as solution) formed after the enzymatic reaction has taken place will also be limited in a later phase. The enzymatic reaction takes place when a first member is bound to a second member of a binding pair, wherein the presence of the relatively hydrophilic patch or patches within the droplet or spot circumference allows the substrate to be positioned, or detectable. The patches disclosed herein may also be described as pixels within the spot(s), wherein the optically detectable or fluorescent substrate will finally be located. If so desired, patches may be hydrophobic where the surrounding area is relatively hydrophilic, when, for example, solutions or (optically detectable) markers are tested of a more hydrophobic nature.
In another exemplary embodiment, the support described herein comprises at least a spot or dot, e.g., a collection of first member molecules such as a nucleic acid or peptide construct, density as large as 25 or 50, even 100, 200, up to 500, or even 1,000 spots per square centimeter. The spots or dots are positionally or spatially addressable, wherein each of the spots or dots cover at least one patch, cover from 3-5 patches, or even 5-15 or more patches or pixels.
Hydrophilic path sizes can be modified by selecting the appropriate support material, such as polyethylene, polypropylene or another relatively hydrophobic plastic material to begin with, or by providing the patch size with patches in the desired size, such as by utilizing print technology. For instance, a support surface may be produced from a relatively hydrophobic polypropylene surface upon which grafts are provided that form the relatively hydrophilic patches. The grafts are made with polyacrylic acid that has an excellent suitable hydrophilic nature and allows for testing under physiological circumstances. The patch size may be influenced by selecting the appropriate roughness or a polyethylene or polypropylene starting material. The roughness can also be modulated by sanding, polishing, any other mechanical (printing) or chemical (etching) method, or combinations thereof to modulate a surface on which the hydrophilic patches are to be generated. The smaller the hydrophilic patch size, the smaller the droplets can be applied, such as up to the size where at least one patch falls within the circumference of the applied droplets.
A method for determining binding of a first member molecule within a library of tentative first member binding molecules with a second member binding molecule is also disclosed. The method includes providing a support with spots comprising the tentative first member binding molecules, providing a second member binding molecule and detecting binding of a first member molecule with the second member binding molecule.
The binding is detected with an optically detectable marker, such as a fluorophore, that is directly or indirectly labeled to a probe. The probe may be a nucleic acid or an antibody and, thus, allows a support of the present invention to be used in any type of micro-array. By preventing diffusion, problems such as signal overload can be avoided or circumvented. Thus, one exemplary embodiment of the invention discloses a method wherein binding pairs are detected via enzyme-linked-assay techniques where diffusion or leakage can be a problem. By preventing diffusion, the enzymatic detection method disclosed herein is more sensitive and allows fewer copies of tentative first member molecules to be spotted on one spot, thus decreasing spot-size and increase spot density without losing sensitivity. The enzymatic detection may be up to 10-1,000 times more sensitive than detection using directly labeled probes.
Suitable enzyme-substrate combinations and methods for use in exemplary embodiments of the invention are, for example, found with U.S. Pat. No. 4,931,223 that discloses processes in which light of different wavelengths is simultaneously released from two or more enzymatically decomposable chemiluminescent 1,2-dioxetane compounds. The compounds are configured by means of the inclusion of a different light emitting fluorophore in each compound, such that each compound emits light of the different wavelengths by decomposing each of the compounds with a different enzyme. Also, Bronstein et al., BioTechniques 12 #5 (May 1992) pp. 748-753, “Improved Chemiluminescent Western Blotting Procedure” discloses an assay method in which a member of a specific binding pair is detected with an optically detectable reaction. The reaction includes an enzyme of a dioxetane such that the enzyme cleaves an enzyme-cleavable group from the dioxetane to form a negatively charged substituent bonded to the dioxetane. The negatively charged substituent causes the dioxetane to decompose and form a luminescent substance. Cano et al., J. App. Bacteriology 72 (1992) discloses an example of nucleic acid hybridization with a fluorescent alkaline phosphatase substrate that can also be used in the present invention, and Evangelista et al., Anal. Biochem. 203 (1992) teaches alkyl-and aryl-substituted salicyl phosphates as detection reagents in enzyme amplified fluorescence DNA hybridization assays.
As will be described herein, a fluorescent substrate for alkaline phosphatase-based detection of protein blots is used with fluorescence scanning equipment, such as Molecular Dynamics Fluorlmager or Storm instruments, generally known as Vistra ECF and typically deemed suitable for use in Western blotting, and dot and slot blotting applications. The enzymatic reaction of alkaline phosphatase dephosphorylates the ECF substrate to produce a fluorescent product which is detectable in a method of the invention. The invention also discloses a method wherein a substrate for evaluating glycosidic enzymes comprising a fluorescein derivative, such as disclosed in U.S. Pat. No. 5,208,148, is used. The substrate bears a lypophilic character and will reside in hydrophobic areas of the surface.
Another exemplary embodiment of the invention discloses a synthetic molecule comprising a binding site, i.e., located on the detected first member molecule or derivatives thereof, or a binding molecule including a binding site identifiable or obtainable by a method according to the invention. In a further exemplary embodiment of the invention, the support or methods may be used for identifying or obtaining a synthetic molecule having a binding site, or for identifying or obtaining a binding molecule capable of binding to a binding site, and the use of such an obtained molecule for interfering with or effecting binding to a binding molecule.
The micro-array support and methods of the present invention may be used to detect binding of peptides, nucleic acids or other biomolecules. Conventional Pepscan methods use pins (Geysen et al.) or wells (Slootstra et al.). Polyacrylic acid grafts or other acrylic grafts on the polyethylene pins or in the polypropylene wells were used as carriers of peptides. Due to the high peptide loadings (each other carbon atom of the polymer can in theory carry a peptide) tested in an ELISA format, extreme low binding-interactions of a peptide to an antibody can be detected (detection of kD<3 M are possible). In this system, the interactions were separated physically, i.e., by walls of wells. Technically, miniaturization of this concept stops at approximately 10 wells/cm2 due to the limitations of conventional (syringe /needle) liquid handling techniques in practice. When the set-up is miniaturized, it is desirable to keep the two strongholds (high peptide loadings in combination with enzyme-linked detection methods) intact.
Rough polypropylene (PP) supports are commercially available and are widely used as non-shiny material in all sorts of applications. The rough PP appeared to be an ideal template for attaching polyacrylic acid grafts. For example, microscope viewing of PP (EVACAST 1070 N16; Vink Kunststoffen BV) surfaces reveal rounded elevations (hills) separated by tiny depressions (valleys) (See,
The pattern of hydrophilic (normally hydrophilic matrixes cause severe diffusion) and hydrophobic areas (blocks diffusion) diminish diffusion, especially when the patches are smaller then the droplet size of dispensed material. Although the surface of the PP is not completely covered with a homogenous graft, high loadings of peptide/cm2 are possible due to the relatively high surface occupation of the polyacrylic acid grafts on the PP surfaces. In the above described setup, thicker grafts can carry higher peptide loadings, but will suffer from more diffusion problems of dispensed material because of the growing occupation of grafted surface. However, the material can be made to suit various needs as regard to loading versus diffusion.
Enzyme-linked assays use substrates which are converted by the enzyme in products that precipitate in situ or are water soluble. A drawback of precipitating products is the non-reusability of the system caused by insolubility of the precipitated material during cleaning. The set up that makes use of non precipitating products, in particular non precipitating products which are fluorescent, because of the ease of detection by modern fluorescent signal detecting applications is desirable. When substrates (developing soluble products) are put on the surface, such as where excess of substrate material is in a later stage removed from the surface, dye development does not suffer from diffusion problems. This phenomenon is caused by the valley/hill or hydrophobic/hydrophilic construction of the surface in combination with excellent wettability properties of the polyacrylic acid matrix.
A polypropylene (PP) support (EVACAST 1070 N16; Vink Kunststoffen BV) was grafted with acrylic acid to introduce polyacrylic acid grafts on the PP surface. In this case, the solid PP support was irradiated in the presence of 6%, 9% or 12% acrylic acid solutions in water containing CuSO4 using gamma radiation at a dose of 12, 30 or 50 kGy (combinations: 6% acrylic acid and 12kGy=6/12 Ac; 9% acrylic acid with 30 kGy=9/30 Ac and 12% acrylic acid with 50 kGy=12/50 Ac). The grafted solid support containing carboxylic acid groups was functionalized with amino groups via coupling of t-butyloxycarbonylhexamethylenediamine (Boc-HMDA) using dicyclohexylearbodiimide (DCC) with N-hydroxybenztriazole (HOBt) and subsequent cleavage of the Boc groups using trifluoracetic acid. To introduce a thiol reactive bromacetamide group on the support, the amino group functionalized support was treated with bromoacetic acid using DCC or DCC/HOBt.
Peptides containing cysteine residues were able to couple to the bromo functionalized surface via the thiol group of the cysteine residues forming a stable thioether bond. Peptides were spotted on the bromo functionalized surface using gridding pins (Genomic Solutions) with different diameters (1.5 mm, 0.8 mm, 0.6 mm, 0.4 mm and 0.25 mm). Solutions with different concentrations of peptide were used (1 mg/ml, 0.2 mg/ml, 0.04 mg/ml and 0.008 mg/ml). When aliquots of peptide solutions (in bicarbonate buffer at about pH 7-8) were dispensed on the support using the gridding pins, the coupling of the bromo group on the surface to the thiol group of the peptide was achieved in a humid chamber (overnight reaction). Extensive washing removed uncoupled peptide.
Peptides used included: GCASLQGMDTCGK (Nr1) (SEQ ID NO: 2), CAFKQGVDTCGK (Nr2) (SEQ ID NO: 3), APDPFQGVDTCGK (Nr3) (SEQ ID NO: 4), and GCAPDPFQGVDTCGK (Nr4) (SEQ ID NO: 5). From surface plasmon resonance (SPR) measurements, affinity constants are known with antibody Mab GO1: Nr1 kD=<10-3; Nr2 kD=3.10-7; Nr3 kD=4.10-6; and Nr4 kD=6.10-8.
Binding of the antibody to the peptides was detected using a method that made use of a fluorescent product. The whole PP support containing the peptide functionalized areas was incubated with the antibody (Mab GO1 5 ug/ml, incubation overnight). After washing, a subsequent incubation of a second anti-mouse antibody conjugated to alkaline phosphatase introduces, after binding of the Mab to the peptide, the enzyme alkaline phosphatase at the peptide functionalized surface (spots). After washing, the bound enzyme caused fluorescent product signals at the peptide functionalized surfaces when a thin film of a Vistra ECF substrate (Amersham Pharmnacia Biotech) solution was added to the surface (excess substrate was removed). Fluorescent product signals could be quantified on a Storm (Molecular Dynamics) in blue fluorescent mode.
Glucose Oxidase. A polypropylene (PP) support (EVACAST 1070 N16; Vink Kunststoffen BV) was grafted with polyacrylic acid. The solid support was irradiated in the presence of 6% acrylic acid solution in water containing CuSO4 using gamma radiation at a dose of 12kGy. The grafted solid support containing carboxylic acid groups was functionalized with amino groups via coupling of t-butyloxycarbonylhexamethylenediamine (Boc-HMDA) using dicyclohexylcarbodiimide (DCC) with N-hydroxybenztriazole (HOBt) and subsequent cleavage of the Boc groups using trifluoracetic acid. To introduce a thiol reactive bromacetamide group on the support, the amino group functionalized support was treated with bromoacetic acid using DCC or DCC/HOBt.
Glucose oxidase containing thiol-groups (Glu-ox-SH) was able to couple to the bromo functionalized surface. Thiol groups on Glucose oxidase (Glu-ox; 1 mg/ml) were introduced in 0.16 M borate buffer (pH 8) using 2-iminothiolane (5 times molar excess 2-iminothiolane over Glu-ox; 45 min at room temperature). Glu-ox-SH was spotted on the bromo functionalized surface using gridding pins (Genomic Solutions) with different diameters (1.5 mm, 0.8 mm, 0.6 mm, 0.4 mm and 0.25 mm). Concentration of Glu-ox-SH was 0.25 mg/ml. When aliquots of Glu-ox-SH solutions (in phosphate buffered saline (PBS), 1 mM Titriplex=EDTA at pH 7) were dispensed on the support using the gridding pins, the coupling of the bromo group of the surface to the thiol group of Glu-ox-SH was achieved in a humid chamber (overnight reaction). Extensive washing removed uncoupled Glu-ox-SH.
Binding of an antibody (Mab G01) to Glu-ox was detected using a method that made use of a fluorescent product. The whole PP support containing the Glu-ox functionalized areas was incubated with the antibody GO1 (5 ug/ml). After washing, a subsequent incubation of a second anti mouse antibody conjugated to alkaline phosphatase introduces, after binding of the Mab to Glu-ox, the enzyme alkaline phosphatase at the Glu-ox functionalized surface (spots). After washing, the bound enzyme caused fluorescent product signals at the peptide functionalized surfaces when Vistra ECF substrate (Amersham Pharmacia Biotech) (excess substrate was removed) was introduced. Fluorescent product signals could be quantified on a Storm (Molecular Dynamics) in blue fluorescent mode.
Head-to-tail matrix-scan. In a complete matrix-scan, the N-terminal sequence of, for instance, sequence (1-11) of a protein is linked as a building block with each overlapping peptide sequence of a complete scan of the same protein as shown in
(Type II): Tail-to-tail matrix-scan. This is the same scan as the complete matrix scan from Example 3A, however, in this scan, the cysteine of the second building block is located at its N-terminus and provides a reversed or tail-to-tail orientation of both building blocks in the construct as shown in
Multi building block scan. In this example, a thiol function is introduced on an amino-functionalized solid support. This can be made by a direct reaction of the amino groups with, for instance, iminothiolane, or by coupling of Fmoc-Cys(Trt)-OH, followed by Fmoc cleavage using piperidine, acetylation, and trityl deprotection using TFA/scavenger mixtures. This thiol-functionalized solid support can be reacted with, for instance, a bromoacetamide-peptide, containing a protected cysteine residue. After coupling of the first peptide, the cysteine can be deprotected using, for instance, a TFA/scavenger mixture. The formed free thiol group can be used to couple a second bromoacetamide-peptide, again containing a protected cysteine. This procedure can be repeated to make multi-building block constructs. Several types of scans, as described in the other examples, can be used in combination with this multi building block scan. In
| Number | Date | Country | Kind |
|---|---|---|---|
| 01200551.8 | Feb 2001 | EP | regional |
This application is a continuation of application Ser. No. 10/642,553, filed Aug. 14, 2003, pending, which is a continuation of PCT/NL02/00097, filed Feb. 15, 2002, designating the United States of America, corresponding to PCT International Publication WO 02/066984 (published in English on Aug. 29, 2002), the contents of each of which are incorporated herein in their entirety.
| Number | Date | Country | |
|---|---|---|---|
| Parent | 10642553 | Aug 2003 | US |
| Child | 11595775 | Nov 2006 | US |
| Parent | PCT/NL02/00097 | Feb 2002 | US |
| Child | 10642553 | Aug 2003 | US |
