Patent Application
20130100537
This application claims the benefit of German Patent Application DE 102011116757.2 filed Oct. 20, 2011 the entire contents of which are incorporate by reference herein.
The invention relates to a planapochromatically corrected immersion microscope objective for high-resolution microscopy applications with changing dispersive immersion conditions, having a plurality of lenses and/or subsystems comprising lens groups and a corrective function for eliminating spherical aberrations.
In high-quality microscopy, it is of great significance that the object information is transmitted to the image plane free of so-called image errors (aberrations). If deviations from the calculated ideal conditions occur, aberrations are generated. One form of such aberrations are, e.g., necessary system tolerances, which are unavoidable for the manufacture of a cost-efficient microscope. The aberrations generated by the tolerances are, as a rule, eliminated with suitable adjustment options. However, during the everyday use of the microscope, there are also influences on the ideal conditions that cannot be changed with adjustments and lead to aberrations, which visibly degrade the optical image. Examples of such aberrations are thermal influences or tolerance deviations of the cover glasses to be used. For example, a deviation of the cover glass thickness by merely 0.01 mm can lead to a significant spherical aberration in high-aperture microscope objectives.
For applications in “live cell” microscopy, it is advantageous to operate with temperatures of up to 37 degrees Celsius. Once again, spherical aberrations occur. In order to create good working conditions for these applications as well, microscope objectives with a corrective function for eliminating the spherical aberration have been developed. This corrective function is realized using adjustable air gaps or air gap combinations between the optical elements of the microscope objective. E.g., such a solution is introduced in U.S. Pat. No. 5,940,220. Here, the distance changes are only allowed to influence the image error, which is generated by the type of application and supposed to be eliminated. This requires that such distance effects be specifically produced during the development phase of the optical system.
In modern microscopy, focusing into the sample is the increasingly preferred method. This changes the immersion condition, for which a special objective has been developed. Due to the spectral changes of the refractive index between the immersion and the sample, significant longitudinal chromatic aberrations, in addition to spherical aberrations, can occur even in the axis region which destroy the achromatic and apochromatic properties of a microscope objective, and therefore considerably degrade the chromatic image.
The same condition occurs when objectives with different immersions are used. Once the dispersive properties of the immersions deviate from one another, an additional longitudinal chromatic aberration is generated and the achromatic and apochromatic properties of a microscope objective are therefore also lost.
With the known corrective microscope objectives, which are designed for constant immersion conditions, the chromatic aberrations in the image cannot be eliminated. Proceeding from the disadvantages of the solution of the prior art, the invention addresses the problem of further developing a planapochromatically-corrected immersion microscope objective for high-resolution microscopy applications such that the spherical aberration errors and the longitudinal chromatic aberrations are eliminated in case of dispersive changes in the immersion and sample region (double-corrective microscope objective).
According to the invention, this problem is solved with a planapochromatic microscope objective of the initially described type through the features described herein.
According to the invention, the microscope objective has an additional corrective function for eliminating longitudinal chromatic aberrations caused by dispersive changes in the immersion by changing the air gaps between the lenses or gap combinations, wherein the influence on the longitudinal chromatic aberration corresponds to a rotation of the curve s(λ) which describes the color point s as a function of the wavelength λ.
Proceeding from the object plane, the microscope objective according to an example embodiment comprises three subsystems, wherein
The corrective function eliminates spherical aberrations through a change of the Nijboer-Zernike coefficient of the 3rd order, a wavefront described by Nijboer-Zernike polynomials for the optical imaging of a point.
As a result of the two corrective functions, spherical aberrations, as well as longitudinal chromatic aberrations, can be eliminated with the microscope objective allowing for high-resolution microscopic examinations at changed immersions with only one objective. Furthermore, it is possible to focus into samples with different dispersive properties without losing the apochromatic function.
Advantageously, adjusting the air gap between the first lens and the second lens of the first subsystem enables the corrective function for changing the longitudinal chromatic aberration, wherein adjustments for changing the longitudinal chromatic aberration of ±0.17 mm are possible.
In an example embodiment, the adjustment of the air gap between the second lens of the first double-cemented element and the first lens of the second double-cemented element enables the corrective function of the spherical aberration for changing the Nijboer-Zernike coefficient of the 3rd order.
Expediently, mechanical, manually operatable means are provided for changing the air gaps for the purpose of adjusting the corrective functions.
It is also conceivable to couple the objective with a control device for generating suitable air gaps, and, using target functions of optics programs, said control device would allow for recording and controlling the course characteristics of the air gaps.
The “double-corrective microscope objective”, according to an example embodiment of the invention, is diffraction limited and apochromatically corrected, has an image field of 23 mm, a magnification of 20 and an aperture facing the object of 1.0 at a mean immersion refractive index of 1.3827 (slightly variable immersion). Despite the large aperture, the free working distance has a length of 5.6 mm at a parfocal length of 105 mm.
In the following, the planapochromatically-corrected microscope according to the invention is further described in terms of one embodiment:
The subsystem T1 comprises two single lenses L2 and L3, wherein both lenses are collecting lenses bent in the direction of the object plane OE. L1 denotes the immersion means.
The second subsystem T2 has a double-cemented element with the lenses L4 and L5, a further double-cemented element with the lenses L6 and L7, and a triple-cemented element with the lenses L8, L9, and L10, wherein the lenses L4, L6, and L8, which are oriented in the direction of the object plane OE, are collecting lenses.
The third subsystem T3 comprises a double-cemented element with the lenses L11 and L12, and a triple-cemented element with the lenses L13, L14, and L15, wherein the concave surface of the lens L12 of the double-cemented element is facing the image, and the concave surface of the lens L13 of the triple-cemented element is oriented in the direction of the object plane OE.
The corrective function LA2 eliminates spherical aberrations by changing the air gap d8 in the adjusting range of the Nijboer-Zernike coefficient of the 3rd order.
The corrective function LA1 is used for adjusting the longitudinal chromatic aberration by rotating the curve s(λ), which describes the color point s as a function of the wavelength λ. For that purpose, the air gap d3 is adjustable within an adjusting range of ±0.17 mm.
A slightly variable, water-like immersion, an image field of 23 mm, a magnification of 20, an aperture of 1.0 facing the object, a free working distance of 5.6 mm and a parfocal length of 105 mm result in the following design data for the microscope objective, wherein r0 to r21 denote the radii of curvature in mm, d1 to d21 denote the thicknesses or air gaps in mm, ye denotes the Abbe numbers, and ne denotes the refractive indices:
with the following design data of the tube lens system, wherein r1T to r6T denote the radii of curvature in mm, d1T to d5T denote the thicknesses or air gaps in mm, ye denotes the Abbe numbers, and ne denotes the refractive indices:
The invention may also include mechanical, manually operatable control structures for changing the air gaps (d3, d8) for the purpose of adjusting the corrective functions (LA1, LA2).
According to another example embodiment, the invention may include a control device coupled with the objective that generates suitable air gaps. The control device may include using target functions of optics programs. The control device may also allow for recording and controlling the course characteristics of the air gaps (d3, d8).
The present invention may be embodied in other specific forms without departing from the spirit of the essential attributes thereof; therefore, the illustrated embodiments should be considered in all respects as illustrative and not restrictive, reference being made to the appended claims rather than to the foregoing description to indicate the scope of the invention.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10 2011 116 757.2 | Oct 2011 | DE | national |
