Claims
- 1. A transgenic plant cell containing a DNA molecule encoding Streptococcus mutans glucosyltransferase D enzyme, wild type or mutant, wherein the mutant is T589D; T589E; N471D; N471D/T589D; or N471D/T589E, and wherein the plant cell is derived from a plant selected from the group consisting of potato, cassava and sweet potato.
- 2. The plant cell of claim 1 which is transformed by Agrobacterium tumefaciens, electroporation, retroviruses, bombardment or microinjection.
- 3. The plant cell of claim 1 wherein the enzyme produces a glucan in the amyloplast or vacuole of the plant cell.
- 4. A transgenic plant regenerated from the plant cell of claim 1.
- 5. A transgenic plant seed containing a DNA molecule encoding Streptococcus mutans glucosyltransferase D enzyme, wild type or mutant, wherein the mutant is T589D; T589E; N471D; N471D/T589D; or N471D/T589E and wherein the plant seed is derived from a plant selected from the group consisting of maize, rye, barley, wheat, sorghum, oats, millet, triticale and rice.
- 6. The maize seed of claim 5 wherein the seed is from a maize line deficient in starch biosynthesis.
- 7. The seed of claim 6 wherein the plant is maize of genotype sh.sub.2, bt.sub.2 or bt.sub.1.
- 8. The seed of claim 6 wherein the enzyme produces an insoluble product.
- 9. The plant seed of claim 5 wherein the DNA molecule contains a promoter selected from the group consisting of 22 kDa zein, opaque2, gamma zein and waxy gene promoters.
Parent Case Info
This application is a divisional of U.S. patent application Ser. No. 08/482,711, filed on Jun. 7, 1995, now abandoned.
US Referenced Citations (9)
Foreign Referenced Citations (4)
Number |
Date |
Country |
6 -313297 |
Nov 1994 |
JPX |
6-287887 |
Nov 1994 |
JPX |
1122354 |
Aug 1968 |
GBX |
WO 9513389 |
May 1995 |
WOX |
Divisions (1)
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Number |
Date |
Country |
Parent |
482711 |
Jun 1995 |
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