Claims
- 1. A method for obtaining a centromere nucleic acid sequence from a selected organism, the method comprising the steps of:
preparing a sample of genomic DNA from a selected organism; obtaining a plurality of nucleic acid segments from the genomic DNA; and screening the nucleic acid segments to identify one or more centromere nucleic acid sequences.
- 2. The method of claim 1, wherein the step of obtaining comprises contacting said genomic DNA with a restriction endonuclease and selecting nucleic acid segments containing repetitive DNA to obtain said plurality of nucleic acid segments.
- 3. The method of claim 1, wherein said the step of obtaining comprises contacting said genomic DNA with a methylation sensitive restriction endonuclease and selecting nucleic acid segments exhibiting resistance to cleavage with said methylation sensitive restriction endonuclease to obtain said plurality of nucleic acid segments.
- 4. The method of claim 1, wherein said obtaining comprises fragmenting said genomic DNA and selecting nucleic acid segments that anneal rapidly after denaturation to obtain said plurality of nucleic acid segments.
- 5. The method of claim 1, wherein the selected organism is a plant.
- 6. The method of claim 5, wherein the plant is a dicotyledonous plant.
- 7. The method of claim 6, wherein the dicotyledonous plant is selected from the group consisting of tobacco, tomato, potato, sugar beet, pea, carrot, cauliflower, broccoli, soybean, canola, sunflower, alfalfa, and cotton.
- 8. The method of claim 6, wherein the plant is a monocotyledonous plant.
- 9. The method of claim 8, wherein the monocotyledonous plant is selected from the group consisting of wheat, maize, rye, rice, turfgrass, oat, barley, sorghum, millet, and sugarcane.
- 10. A method for identifying a centromere nucleic acid sequence from an organism, the method comprising the steps of:
providing a first dataset consisting of genomic sequences of the organism wherein the dataset is selected from the group consisting of total genome datasets, and representative genome datasets; identifying and eliminating known non-centromeric repeat sequences from the first dataset by using a BLAST sequence comparison algorithm to create a second dataset; comparing each sequence in the second dataset to itself by using the BLAST sequence comparison algorithm, obtaining a BLAST score for each pair of sequence compared, and collecting high score pairs to create a third dataset; examining the BLAST score of each high score pair in the third dataset and eliminating the pairs having a score greater than 10−20 to create a fourth dataset; eliminating the high score pairs in the fourth dataset having less than 80 bp or more than 250 bp to create a fifth dataset; examining the nucleotide position of each high score pair in the fifth dataset and eliminating pairs having 100% identity and identical nucleotide positions to create a sixth dataset; examining the nucleotide position of each high score pair in the sixth dataset and eliminating pairs having opposite orientation of the nucleotides to create a seventh dataset; examining the nucleotide position of both sequences for each high score pair in the seventh dataset and eliminating sequences that are overlapping to create an eighth dataset; and examining the nucleotide position of each sequence in the eighth dataset and eliminating sequences not having at least one neighboring sequence within 250 bp to create a ninth dataset; and comparing each sequence in the ninth dataset to all other sequences in the ninth dataset by using the BLAST sequence comparison algorithm and selecting the most common sequence as a centromere sequence of the organism.
- 11. The method of claim 10, wherein the known non-centromeric repeat sequence in the second step is a ribosomal DNA.
- 12. The method of claim 10, wherein the steps occur in the listed order.
- 13. A method for identifying centromere sequences comprising the steps of using a BLAST algorithm to identify centromere sequences from a genomic sequence database.
- 14. An isolated nucleic acid comprising a plant centromere having n copies of a repeated nucleotide sequence isolated from total genomic DNA of the plant, wherein n is at least 2.
- 15. The isolated nucleic acid of claim 14, wherein the repeated nucleotide sequence is provided by any circular permutation of the repeated nucleotide sequence.
- 16. The isolated nucleic acid of claim 14, wherein n is from about 5 to about 100,000.
- 17. The isolated nucleic acid of claim 14, wherein n is from about 10 to about 80,000.
- 18. The isolated nucleic acid of claim 14, wherein n is from about 25 to about 60,000.
- 19. The isolated nucleic acid of claim 14, wherein n is from about 100 to about 50,000.
- 20. The isolated nucleic acid of claim 14, wherein n is from about 200 to about 40,000.
- 21. The isolated nucleic acid of claim 14, wherein n is from about 400 to about 30,000.
- 22. The isolated nucleic acid of claim 14, wherein n is from about 1,000 to about 30,000.
- 23. The isolated nucleic acid of claim 14, wherein n is from about 5,000 to about 20,000.
- 24. The isolated nucleic acid of claim 14, wherein n is from about 10,000 to about 15,000.
- 25. An isolated nucleic acid comprising a Brassica oleracea centromere, wherein the centromere comprises n copies of a repeated nucleotide sequence isolated from Brassica oleracea, and wherein n is at least 2.
- 26. The isolated nucleic acid of claim 25, wherein the repeated nucleotide sequence is given by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4.
- 27. The isolated nucleic acid of claim 25, wherein the repeated nucleotide sequence is provided by any circular permutation of the repeated nucleotide sequence.
- 28. The isolated nucleic acid of claim 25, wherein n is from about 5 to about 100,000.
- 29. The isolated nucleic acid of claim 25, wherein n is from about 10 to about 80,000.
- 30. The isolated nucleic acid of claim 25, wherein n is from about 25 to about 60,000.
- 31. The isolated nucleic acid of claim 25, wherein n is from about 100 to about 50,000.
- 32. The isolated nucleic acid of claim 25, wherein n is from about 200 to about 40,000.
- 33. The isolated nucleic acid of claim 25, wherein n is from about 400 to about 30,000.
- 34. The isolated nucleic acid of claim 25, wherein n is from about 1,000 to about 30,000.
- 35. The isolated nucleic acid of claim 25, wherein n is from about 5,000 to about 20,000.
- 36. The isolated nucleic acid of claim 25, wherein n is from about 10,000 to about 15,000.
- 37. An isolated nucleic acid comprising a Glycine max centromere, wherein the centromere comprises n copies of a repeated nucleotide sequence isolated from Glycine max, and wherein n is at least 2.
- 38. The isolated nucleic acid of claim 33, wherein the repeated nucleotide sequence is given by SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8.
- 39. The isolated nucleic acid of claim 37, wherein the repeated nucleotide sequence is provided by any circular permutation of the repeated nucleotide sequence.
- 40. The isolated nucleic acid of claim 37, wherein n is from about 5 to about 100,000.
- 41. The isolated nucleic acid of claim 37, wherein n is from about 10 to about 80,000.
- 42. The isolated nucleic acid of claim 37, wherein n is from about 25 to about 60,000.
- 43. The isolated nucleic acid of claim 37, wherein n is from about 100 to about 50,000.
- 44. The isolated nucleic acid of claim 37, wherein n is from about 200 to about 40,000.
- 45. The isolated nucleic acid of claim 37, wherein n is from about 400 to about 30,000.
- 46. The isolated nucleic acid of claim 37, wherein n is from about 1,000 to about 30,000.
- 47. The isolated nucleic acid of claim 37, wherein n is from about 5,000 to about 20,000.
- 48. The isolated nucleic acid of claim 37, wherein n is from about 10,000 to about 15,000.
- 49. An isolated nucleic acid comprising a Lycopersicon esculentum centromere, wherein the centromere comprises n copies of a repeated nucleotide sequence isolated from Lycopersicon esculentum, and wherein n is at least 2.
- 50. The isolated nucleic acid of claim 49, wherein the repeated nucleotide sequence is given by SEQ ID NO:9 or SEQ ID:10.
- 51. The isolated nucleic acid of claim 49, wherein the repeated nucleotide sequence is provided by any circular permutation of the repeated nucleotide sequence.
- 52. The isolated nucleic acid of claim 49, wherein n is from about 5 to about 100,000.
- 53. The isolated nucleic acid of claim 49, wherein n is from about 10 to about 80,000.
- 54. The isolated nucleic acid of claim 49, wherein n is from about 25 to about 60,000.
- 55. The isolated nucleic acid of claim 49, wherein n is from about 100 to about 50,000.
- 56. The isolated nucleic acid of claim 49, wherein n is from about 200 to about 40,000.
- 57. The isolated nucleic acid of claim 49, wherein n is from about 400 to about 30,000.
- 58. The isolated nucleic acid of claim 47, wherein n is from about 1,000 to about 30,000.
- 59. The isolated nucleic acid of claim 47, wherein n is from about 5,000 to about 20,000.
- 60. The isolated nucleic acid of claim 47, wherein n is from about 10,000 to about 15,000.
- 61. An isolated nucleic acid sequence comprising a Zea mays centromere, wherein the centromere comprises n copies of a repeated nucleotide sequence isolated from Zea mays, and wherein n is at least 2.
- 62. The isolated nucleic acid sequence of claim 61, wherein the repeated nucleotide sequence is give by SEQ ID NO:11, SEQ ID NO: 12 or SEQ ID NO:13.
- 63. The isolated nucleic acid sequence of claim 61, wherein the repeated nucleotide sequence is provided by any circular permutation of the repeated nucleotide sequence.
- 64. The isolated nucleic acid of claim 61, wherein n is from about 5 to about 100,000.
- 65. The isolated nucleic acid of claim 61, wherein n is from about 10 to about 80,000.
- 66. The isolated nucleic acid of claim 61, wherein n is from about 25 to about 60,000. The isolated nucleic acid of claim 61, wherein n is from about 100 to about 50,000.
- 67. The isolated nucleic acid of claim 61, wherein n is from about 200 to about 40,000.
- 68. The isolated nucleic acid of claim 61, wherein n is from about 400 to about 30,000.
- 69. The isolated nucleic acid of claim 61, wherein n is from about 1,000 to about 30,000.
- 70. The isolated nucleic acid of claim 61, wherein n is from about 5,000 to about 20,000.
- 71. The isolated nucleic acid of claim 61, wherein n is from about 10,000 to about 15,000.
- 72. A recombinant DNA construct comprising a plant centromere comprising n copies of a repeated nucleotide sequence obtained from total genomic DNA of the plant, and wherein n is at least 2.
- 73. The recombinant DNA construct of claim 72, which additionally comprises a telomere.
- 74. The recombinant DNA construct of claim 72, which additionally comprises an autonomous replicating sequence (ARS).
- 75. The recombinant DNA construct of claim 72, which additionally comprises a selectable marker gene.
- 76. The recombinant DNA construct of claim 72, which additionally comprises at least a structural gene.
- 77. The recombinant DNA construct of claim 76, wherein the structural gene is selected from the group consisting of a selectable or screenable marker gene, an antibiotic resistance gene, a ligand gene, an enzyme gene, a herbicide resistance gene, a nitrogen fixation gene, a plant pathogen defense gene, a plant stress-induced gene, a toxin gene, a receptor gene, a gene encoding an enzyme, a gene encoding an antibody, a gene encoding an antigen for a vaccine, a transcription factor, a cytoskeletal protein, a DNA-binding protein, a protease, an endonuclease, a lipid, a seed storage gene, an interleukin gene, a clotting factor gene, a cytokine gene, a growth factor gene and a biosynthetic gene for producing pharmaceutically active proteins, small molecules with medicinal properties, chemicals with industrial utility, nutraceuticals, carbohydrates, RNAs, lipids, fuels, dyes, pigments, vitamins, scents, flavors, vaccines, antibodies, and hormones.
- 78. The recombinant DNA construct of claim 76, wherein the construct is capable of expressing the desired structural gene.
- 79. The recombinant DNA construct of claim 72, which is capable of being maintained as a chromosome, wherein the chromosome is stably transmitted in dividing cells.
- 80. A recombinant DNA construct comprising a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and wherein the repeated nucleotide sequence is given by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, or SEQ ID NO:13.
- 81. A plasmid comprising a recombinant DNA construct, wherein the recombinant DNA comprises a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence obtained from total genomic DNA of the plant, and wherein n is at least 2.
- 82. The plasmid of claim 81, which additionally comprises a telomere.
- 83. The plasmid of claim 81, which additionally comprises an autonomous replicating sequence (ARS).
- 84. The plasmid of claim 81, which additionally comprises a selectable marker gene.
- 85. The plasmid of claim 81, which additionally comprises at least a structural gene.
- 86. The plasmid of claim 85, wherein the structural gene is selected from the group consisting of a selectable or screenable marker gene, an antibiotic resistance gene, a ligand gene, an enzyme gene, a herbicide resistance gene, a nitrogen fixation gene, a plant pathogen defense gene, a plant stress-induced gene, a toxin gene, a receptor gene, a gene encoding an enzyme, a gene encoding an antibody, a gene encoding an antigen for a vaccine, a transcription factor, a cytoskeletal protein, a DNA-binding protein, a protease, an endonuclease, a lipid, a seed storage gene, an interleukin gene, a clotting factor gene, a cytokine gene, a growth factor gene and a biosynthetic gene for producing pharmaceutically active proteins, small molecules with medicinal properties, chemicals with industrial utility, nutraceuticals, carbohydrates, RNAs, lipids, fuels, dyes, pigments, vitamins, scents, flavors, vaccines, antibodies, and hormones.
- 87. The plasmid of claim 85, wherein the construct is capable of expressing the desired structural gene.
- 88. A recombinant DNA construct, wherein the construct comprises a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and wherein the repeated nucleotide sequence is given by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, or SEQ ID NO:13.
- 89. A host transformed with a recombinant DNA construct comprising a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence obtained from total genomic DNA of the plant, and wherein n is at least 2.
- 90. The host of claim 89, wherein the cell is a eukaryotic cell.
- 91. The host of claim 89, wherein the cell is a yeast cell.
- 92. The host of claim 89, wherein the cell is a higher eukaryotic cell.
- 93. The host of claim 92, wherein the cell is a crop cell.
- 94. A host transformed with a recombinant DNA construct comprising a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and wherein the repeated nucleotide sequence is given by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, or SEQ ID NO:13.
- 95. A method of preparing a transgenic plant cell comprising contacting a starting plant cell with a recombinant DNA construct comprising a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence obtained from total genomic DNA of the plant, wherein n is at least 2, and whereby the starting plant cell is transformed with the recombinant DNA construct.
- 96. A method of preparing a transgenic plant cell comprising contacting a starting plant cell with a recombinant DNA construct comprising a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2 and the repeated nucleotide sequence is given by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, or SEQ ID NO:13, and whereby the starting plant cell is transformed with the recombinant DNA construct.
- 97. A minichromosome comprising a plant centromere, wherein the plant centromere comprises n copies of a repeated nucleotide sequence obtained from total genomic DNA of the plant, and wherein n is at least 2.
- 98. The minichromosome of claim 97, further comprising a telomere.
- 99. The minichromosome of claim 97, further comprising an autonomous replicating sequence.
- 100. The minichromosome of claim 97, further comprising a structural gene.
- 101. The minichromosome of claim 100, wherein the structural gene is selected from the group consisting of a selectable or screenable marker gene, an antibiotic resistance gene, a ligand gene, an enzyme gene, a herbicide resistance gene, a nitrogen fixation gene, a plant pathogen defense gene, a plant stress-induced gene, a toxin gene, a receptor gene, a gene encoding an enzyme, a gene encoding an antibody, a gene encoding an antigen for a vaccine, a transcription factor, a cytoskeletal protein, a DNA-binding protein, a protease, an endonuclease, a lipid, a seed storage gene, an interleukin gene, a clotting factor gene, a cytokine gene, a growth factor gene and a biosynthetic gene for producing pharmaceutically active proteins, small molecules with medicinal properties, chemicals with industrial utility, nutraceuticals, carbohydrates, RNAs, lipids, fuels, dyes, pigments, vitamins, scents, flavors, vaccines, antibodies, and hormones.
- 102. The minichromosome of claim 100, wherein the minichromosome is capable of expressing the desired structural gene.
- 103. A minichromosome comprising a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and wherein the repeated nucleotide sequence is given by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, or SEQ ID NO:13.
- 104. A transgenic crop comprising a crop cell transformed with a minichromosome that comprises a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence, and wherein n is at least 2.
- 105. The transgenic crop of claim 104, wherein the minichromosome further comprises at least one structural gene.
- 106. The transgenic crop of claim 105, wherein the structural gene is selected from the group consisting of a selectable or screenable marker gene, an antibiotic resistance gene, a ligand gene, an enzyme gene, a herbicide resistance gene, a nitrogen fixation gene, a plant pathogen defense gene, a plant stress-induced gene, a toxin gene, a receptor gene, a gene encoding an enzyme, a gene encoding an antibody, a gene encoding an antigen for a vaccine, a transcription factor, a cytoskeletal protein, a DNA-binding protein, a protease, an endonuclease, a lipid, a seed storage gene, an interleukin gene, a clotting factor gene, a cytokine gene, a growth factor gene and a biosynthetic gene for producing pharmaceutically active proteins, small molecules with medicinal properties, chemicals with industrial utility, nutraceuticals, carbohydrates, RNAs, lipids, fuels, dyes, pigments, vitamins, scents, flavors, vaccines, antibodies, and hormones.
- 107. The transgenic crop of claim 104, wherein the crop is a dicotyledonous plant.
- 108. The transgenic crop of claim 105, wherein the dicotyledonous plant is selected from the group consisting of tobacco, tomato, potato, sugar beet, pea, carrot, cauliflower, broccoli, soybean, canola, sunflower, alfalfa, and cotton.
- 109. The transgenic crop of claim 104, wherein the crop is a monocotyledonous plant.
- 110. The transgenic crop of claim 109, wherein the monocotyledonous plant is selected from the group consisting of wheat, maize, rye, rice, turfgrass, oat, barley, sorghum, millet, and sugarcane.
- 111. A transgenic crop comprising a cell transformed with a minichromosome, wherein the minichromosome comprises a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the repeated nucleotide sequence is given by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, or SEQ ID NO:13.
- 112. A method for preparing a transgenic crop tissue, the method comprising the steps of contacting a starting crop tissue with a recombinant DNA construct comprising a plant centromere, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, whereby the starting crop tissue is transformed with the recombinant DNA construct.
- 113. A method for shortening the time required to develop a new crop, the method comprising the steps of providing a plant minichromosome comprising a plant centromere, transforming a plant cell with the minichromosome, and generating a transgenic plant from the plant cell, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the minichromosome comprises at least a structural gene.
- 114. The method of claim 113, wherein the new crop is a vegetable crop.
- 115. The method of claim 113, wherein the new crop is a field crop plant.
- 116. The method of claim 113, wherein the new crop is a fruit and vine crop.
- 117. The method of claim 113, wherein the new crop has at least one improved agricultural trait as compared to existing crops of the same species.
- 118. A method of providing a vegetable crop comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a vegetable crop cell of the vegetable crop, transforming the vegetable crop cell with the plant minichromosome, and generating the vegetable crop from the transformed vegetable crop cell, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the minichromosome comprises at least a structural gene.
- 119. A method of providing a fruit and vine crop comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a fruit and vine crop cell of the fruit and vine crop, transforming the fruit and vine crop cell with the plant minichromosome, and generating the fruit and vine crop from the transformed fruit and vine crop cell, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the minichromosome comprises at least a structural gene.
- 120. A method of providing a field crop comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a field crop cell of the field crop, transforming the field crop cell with the plant minichromosome, and generating the field crop from the transformed field crop cell, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the minichromosome comprises at least a structural gene.
- 121. A method of providing a vegetable crop seed comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a vegetable crop cell of the vegetable crop, transforming the vegetable crop cell with the plant minichromosome, generating the vegetable crop from the transformed vegetable crop cell, fertilizing the vegetable crop, and collecting the seeds from the fertilized vegetable crop, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the minichromosome comprises at least a structural gene.
- 122. A method of providing a fruit and vine crop seed comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a fruit and vine crop cell of the fruit and vine crop, transforming the fruit and vine crop cell with the plant minichromosome, generating the fruit and vine crop from the transformed fruit and vine crop cell, fertilizing the fruit and vine crop, and collecting the seeds from the fertilized fruit and vine crop, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the minichromosome comprises at least a structural gene.
- 123. A method of providing a field crop seed comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a field crop cell of the field crop, transforming the field crop cell with the plant minichromosome, generating the field crop from the transformed field crop cell, fertilizing the field crop, and collecting the seeds from the fertilized field crop, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the minichromosome comprises at least a structural gene.
- 124. A method of providing a crop seed comprising the steps of providing a plant minichromosome comprising a plant centromere, providing a cell of the crop, transforming the cell with the plant minichromosome, generating the crop from the transformed crop cell, fertilizing the crop, and collecting the seeds from the fertilized crop, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the minichromosome comprises at least a structural gene.
- 125. A method for providing a food product, the method comprising the steps of transforming a crop with a minichromosome comprising a plant centromere and a structural gene, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the structural gene is capable of expressing the food product, and extracting the food product from the transgenic crop.
- 126. A method for providing a pharmaceutical product, the method comprising the steps of transforming a crop with a minichromosome comprising a plant centromere and a structural gene, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the structural gene is capable of expressing the pharmaceutical product, and extracting the pharmaceutical product from the transgenic crop.
- 127. A method for providing a chemical product, the method comprising the steps of transforming a crop with a minichromosome comprising a plant centromere and a structural gene, the centromere comprises n copies of a repeated nucleotide sequence, wherein n is at least 2, and the structural gene is capable of expressing the chemical product, and extracting the chemical product from the transgenic crop.
Parent Case Info
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 09/553,231, filed Mar. 13, 2002, which is a continuation of U.S. patent application Ser. No. 09/090,051, filed Jun. 3, 1998, now U.S. Pat. No. 6,156,953 which claims the priority of U.S. Provisional Patent Application Ser. No. 60/048,451, filed Jun. 3, 1997; and U.S. Provisional Patent Application Ser. No. 60/073,741, filed Feb. 5, 1998, both of the disclosures of which are specifically incorporated herein by reference in their entirety. This application is also a continuation-in-part of U.S. patent application Ser. No. 09/531,120, filed Mar. 17, 2000, which claims the priority of U.S. Provisional Application Ser. No. 60/125,219, filed Mar. 18, 1999; U.S. Provisional Application Ser. No. 60/127,409, filed Apr. 1, 1999; U.S. Provisional Application Ser. No. 60/134,770, filed May 18, 1999; U.S. Provisional Application Ser. No. 60/153,584, filed Sep. 13, 1999, U.S. Provisional Application Ser. No. 60/154,603, filed Sep. 17, 1999 and U.S. Provisional Application Ser. No. 60/172,493, filed Dec. 16, 1999, each of which disclosures is specifically incorporated herein by reference in its entirety.
Government Interests
[0002] The government owns rights in the present invention pursuant to U.S. Department of Agriculture Grant No. 96-35304-3491 and Grant No. DE-FC05-920R22072 from the Consortium for Plant Biotechnology Research, National Science Foundation Grant No. 9872641, and Department of Energy Small Business Innovation Research Grants DE-FG02-01ER83163, DE-FG02-01ER83165, and DE-FG02-01ER83166.
Provisional Applications (8)
|
Number |
Date |
Country |
|
60048451 |
Jun 1997 |
US |
|
60073741 |
Feb 1998 |
US |
|
60125219 |
Mar 1999 |
US |
|
60127409 |
Apr 1999 |
US |
|
60134770 |
May 1999 |
US |
|
60153584 |
Sep 1999 |
US |
|
60154603 |
Sep 1999 |
US |
|
60172493 |
Dec 1999 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09090051 |
Jun 1998 |
US |
Child |
09553231 |
Apr 2000 |
US |
Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
09553231 |
Apr 2000 |
US |
Child |
10170912 |
Jun 2002 |
US |
Parent |
09531120 |
Mar 2000 |
US |
Child |
10170912 |
Jun 2002 |
US |