Claims
- 1. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; (b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 32, 47, or 49; (c) a nucleotide sequence that encodes a mature polypeptide having the amino acid sequence set forth in SEQ ID NO: 35; (d) a nucleotide sequence characterized by at least 75% sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; (e) a nucleotide sequence characterized by at least 80% sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; (f) a nucleotide sequence characterized by at least 85% sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; (g) a nucleotide sequence characterized by at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; (h) a nucleotide sequence that comprises the complement of any one of (a) to (g).
- 2. The nucleic acid molecule according to claim 1, wherein the nucleotide sequence is optimized for expression in a plant.
- 3. A DNA construct comprising a nucleotide sequence of claim 1, wherein said nucleotide sequence is operably linked to a promoter that drives expression in a host cell.
- 4. An expression cassette comprising the DNA construct of claim 3.
- 5. A host cell having stably incorporated into its genome at least one DNA construct of claim 3.
- 6. The host cell of claim 5, wherein said host cell is a plant cell.
- 7. A plant having stably incorporated into its genome the DNA construct of claim 3.
- 8. The plant according to claim 7, wherein said plant is a monocot.
- 9. The plant according to claim 7, wherein said plant is a dicot.
- 10. Transformed seed of the plant of claim 7.
- 11. An isolated polypeptide selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 32, 47, or 49; (b) a polypeptide characterized by at least 80% identity to SEQ ID NO: 6, 26, or 28; (c) a polypeptide characterized by at least 85% identity to SEQ ID NO: 8; (d) a polypeptide characterized by at least 95% identity to SEQ ID NO: 2, 4, 10, 12, 14, 16, 18, 20, 22, 24, or 47; (e) a polypeptide characterized by at least 97% identity to SEQ ID NO: 32; and (f) the polypeptide of SEQ ID NO: 49.
- 12. The polypeptide of claim 11, wherein said polypeptide is characterized by defensin-like activity against at least one plant pathogen.
- 13. The polypeptide of claim 12, wherein said plant pathogen is a fungus.
- 14. The polypeptide of claim 13, wherein said fungus is Sclerotinia sclerotiorum.
- 15. The polypeptide of claim 11, wherein said polypeptide is characterized by pesticidal activity against at least one insect pest.
- 16. The polypeptide of claim 11, wherein said polypeptide is characterized by pesticidal activity against at least one nematode.
- 17. The polypeptide of claim 16, where said nematode is the Soybean Cyst Nematode.
- 18. A method for impacting a plant pathogen comprising introducing into a plant or cell thereof at least one nucleotide construct comprising a nucleotide sequence operably linked to a promoter that drives expression of a coding sequence in plant cells, wherein said nucleotide sequence is selected from the group consisting of:
(a) a nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; (b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 32, 47, or 49; (c) a nucleotide sequence that encodes a mature polypeptide having the amino acid sequence set forth in SEQ ID NO:35; (d) a nucleotide sequence characterized by at least 75% sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; (e) a nucleotide sequence characterized by at least 80% sequence identity to the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; (f) a nucleotide sequence characterized by at least 85% sequence identity to the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; (g) a nucleotide sequence characterized by at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 46, or 48; and (h) a nucleotide sequence that comprises the complement of any one of (a) to (g).
- 19. The method of claim 18, wherein said plant produces a polypeptide characterized by defensin-like activity against at least one pathogen.
- 20. The method of claim 19, wherein said pathogen is a fungus.
- 21. The method of claim 20, wherein said fungus is Sclerotinia sclerotiorum.
- 22. The method of claim 18, wherein said plant produces a polypeptide characterized by pesticidal activity against at least one insect pest.
- 23. The method of claim 18, wherein said plant produces a polypeptide characterized by pestcidal activity against at least one nematode.
- 24. The method of claim 23, wherein said nematode is a Soybean Cyst Nematode.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This is a continuation-in-part of U.S. application Ser. No. 10/030,516 filed May 3, 2000; which claims the benefit of PCT Application PCT/US00/11952, filed May 3, 2000, and U.S. Provisional Application No. 60/133,039, filed May 7, 1999, the contents of which are herein incorporated by reference in their entirety.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
10030516 |
Oct 2001 |
US |
Child |
10178449 |
Jun 2002 |
US |