Claims
- 1. A purified polynucleotide, wherein the polynucleotide comprises a nucleic acid encoding a plant DFD kinase polypeptide.
- 2. A purified polynucleotide, wherein the polynucleotide comprises a nucleic acid encoding a plant DFD kinase polypeptide, wherein said nucleic acid comprises a nucleic acid sequence with at least 80% sequence homology to SEQ ID NO: 1.
- 3. The purified polynucleotide acid of claim 1, wherein the nucleic acid has at least 85% homology to SEQ ID NO: 1.
- 4. The purified polynucleotide acid of claim 1, wherein the nucleic acid has at least 90% homology to SEQ ID NO: 1.
- 5. The purified polynucleotide acid of claim 1, wherein the nucleic acid has at least 95% homology to SEQ ID NO: 1.
- 6. The purified polynucleotide acid of claim 1, wherein the nucleic acid has the sequence of SEQ ID NO: 1.
- 7. An isolated polynucleotide capable of hybridization under stringent hybridization conditions to a nucleic acid comprising SEQ ID NO: 1.
- 8. The isolated polynucleotide of claim 7, wherein the stringent hybridization conditions comprise hybridization of the isolated polynucleotide in the presence of 2× SSC/0.1% SDS at about room temperature.
- 9. The isolated polynucleotide of claim 7, wherein the stringent hybridization conditions comprise hybridization of the isolated polynucleotide in the presence of 2× SSC/0.1% SDS at about 42° C.
- 10. The isolated polynucleotide of claim 7, wherein the stringent hybridization conditions comprise hybridization of the isolated polynucleotide in the presence of 2× SSC/0.1% SDS at about 68° C.
- 11. A purified plant DFD kinase polypeptide.
- 12. A purified plant DFD kinase polypeptide, comprising an amino acid sequence with at least 80% sequence homology to SEQ ID NO: 2.
- 13. The polypeptide of claim 12, wherein the amino acid has at least 85% homology to SEQ ID NO: 2.
- 14. The polypeptide of claim 12, wherein the amino acid has at least 90% homology to SEQ ID NO: 2.
- 15. The polypeptide of claim 12, wherein the amino acid has at least 95% homology to SEQ ID NO: 2.
- 16. The polypeptide of claim 12, wherein the amino acid comprises the sequence of SEQ ID NO: 2.
- 17. The polypeptide of claim 12, wherein said plant is Arabidopsis.
- 18. An antibody capable of specifically binding to a protein comprising an amino acid sequence of SEQ ID NO: 2.
- 19. The antibody of claim 18, wherein said antibody specifically binds to a polypeptide comprising at least 10 consecutive amino acids of said protein.
- 20. The antibody of claim 18, wherein the antibody is a monoclonal antibody.
- 21. A recombinant plant kinase comprising a DFD motif.
- 22. An expression vector comprising a polynucleotide according to claim 2.
- 23. The expression vector of claim 22, wherein the vector is a plasmid.
- 24. The expression vector of claim 23, wherein the plasmid is a Ti plasmid of Agrobacterium tumefaciens.
- 25. The expression vector of claim 23, wherein the plasmid is a Ri plasmid of Agrobacterium tumefaciens.
- 26. A host cell containing the expression vector of claim 22.
- 27. A method of producing a genetically modified plant having modulated apical dominance as compared to a wildtype plant, said method comprising:
(a) contacting a plant cell with at least one nucleic acid sequence comprising a nucleic acid encoding a plant DFD kinase polypeptide, said nucleic acid sequence operably associated with a regulatory sequence, to obtain a transformed plant cell; (b) producing a plant from said transformed plant cell; and (c) selecting a plant exhibiting said modulated apical dominance.
- 28. The method of claim 27, wherein said nucleic acid encoding a plant DFD kinase polypeptide has the sequence of SEQ ID NO: 1.
- 29. The method of claim 27, wherein the modulation is early loss of or inhibited apical dominance.
- 30. The method of claim 27, wherein the modulated apical dominance results in darkened green foliage.
- 31. The method of claim 27, wherein the modulated apical dominance results in increased lateral root formation.
- 32. The method of claim 27, wherein the modulated apical dominance results in increased apical dominance.
- 33. The method of claim 27, wherein the modulated apical dominance results in decreased lateral root formation.
- 34. The method of claim 27, further comprising exogenous nucleic acid encoding an Arabidopsis polypeptide selected from the group consisting of AUX1, regulatory subunit A, HOOKLESS1, AXR1, AUX/IAA gene family, and TIR1, and combinations thereof.
- 35. The method of claim 27, wherein the exogenous nucleic acid is operably associated with a regulatory nucleic acid sequence.
- 36. The method of claim 35, wherein the regulatory nucleic acid sequence is a promoter.
- 37. The method of claim 27, wherein the nucleic acid further comprises a selectable marker.
- 38. A plant produced by the method of claim 27.
- 39. Plant tissue derived from a plant produced by the method of claim 27.
- 40. A seed derived from a plant produced by the method of claim 27.
- 41. An assay for identifying plant DFD kinases comprising screening plant nucleic acid to identify a polynucleotide sequence that encodes plant kinase comprising a contiguous DFD amino acid sequence.
- 42. A method for identifying a compound that affects auxin signaling or expression in plants comprising:
incubating the compound and a DFD kinase polypeptide; determining the effect of the compound on auxin signaling or expression; and identifying compounds that affect auxin signaling or expression.
- 43. A method for identifying a compound that binds to DFD kinase polypeptide comprising:
incubating at least one compound and a DFD kinase polypeptide such that a DFD kinase-compound complex is formed; separating the complex from unbound DFD kinase, and isolating the compound from the complex.
- 44. The method of claim 43, further comprising determining the effect of the compound on DFD kinase activity.
- 45. The method of claim 44, wherein the isolated compound is a DFD kinase inhibitor.
- 46. The method of claim 43, wherein said compound is selective for DFD kinases and not DFG kinases.
- 47. The method according to claim 45 wherein the kinase inhibitor inhibits one or more plant kinases.
- 48. The method according to claim 45 wherein the kinase inhibitor inhibits one or more mammalian kinases.
- 49. A method for modulating auxin signaling in a plant cell comprising:
contacting said plant cell with the vector of claim 22 to obtain a transformed plant cell; growing the transformed plant cell under plant forming conditions to produce a plant from said transformed plant cells; and selecting a plant exhibiting said modulated auxin signaling.
- 50. A method for exterminating plants, comprising exposing said plant with a compound identified in claim 43.
- 51. A method for selectively growing plants in the presence of a DFD kinase inhibitor, said method comprising transforming desired plants with a kinase containing a DFG domain, and thereafter exposing plants expressing DFD kinases and said desired plants to DFD kinase inhibitors.
- 52. A plant resistant to DFD kinase inhibitors, wherein said plant is transformed with a DFG domain.
RELATED APPLICATIONS
[0001] This invention claims priority under 35 U.S.C. § 119(e) to co-pending provisional patent application No. 60/167,926, filed Nov. 29, 1999, and which is hereby incorporated by reference in its entirety.
GOVERNMENT RIGHTS
[0002] This invention was made with government support under National Science Foundation (NSF) grant #IBN-9723818, NSF grant #BIR-9627087, and NSF grant #MCB-9631390. The government may have certain rights in the invention.
Provisional Applications (1)
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Number |
Date |
Country |
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60167926 |
Nov 1999 |
US |