Claims
- 1. An isolated nucleic acid comprising a sequence selected from the group consisting of SEQ ID NOS:79-98, or a fragment, region, or cis element of said sequence thereof, said isolated nucleic acid being capable of regulating transcription of an operably linked DNA sequence.
- 2. The isolated nucleic acid of claim 1 wherein the isolated nucleic acid is a promoter.
- 3. The isolated nucleic acid of claim 2 wherein the promoter is a hybrid promoter.
- 4. The isolated nucleic acid of claim 3 wherein said isolated nucleic acid confers enhanced expression of operably linked genes in male reproductive tissues.
- 5. The isolated nucleic acid of claim 4 wherein said isolated nucleic acid confers enhanced expression of operably linked genes in anthers.
- 6. The isolated nucleic acid of claim 5 wherein said isolated nucleic acid confers enhanced expression of operably linked genes in wheat anthers.
- 7. The isolated nucleic acid of claim 4 further comprising a minimal promoter.
- 8. The isolated nucleic acid of claim 7 wherein the minimal promoter is selected from the group consisting of a minimal CaMV and a rice actin promoter.
- 9. The isolated nucleic acid of claim 8 wherein the minimal promoter is a minimal CaMV 35S promoter.
- 10. A promoter comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS: 79-98 and fragments thereof.
- 11. The promoter of claim 10 wherein said promoter confers enhanced expression of operably linked genes in male reproductive tissues.
- 12. The promoter of claim 11 wherein said promoter confers enhanced expression of operably linked genes in anthers.
- 13. The promoter of claim 12 wherein said promoter confers enhanced expression of operably linked genes in wheat anthers.
- 14. A cell comprising a DNA construct comprising an isolated nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98 or a fragment, region, or cis element of said sequence thereof, and operably linked to said nucleic acid sequence, a transcribable DNA sequence and a 3′ non-translated region.
- 15. A transgenic plant comprising a DNA construct comprising an isolated nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98 or a fragment, region, or cis element of said sequence thereof, and operably linked to said nucleic acid sequence, a transcribable DNA sequence and a 3′ non-translated region.
- 16. A method of regulating transcription of a DNA sequence comprising operably linking the DNA sequence to a promoter comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98.
- 17. The method of claim 16 comprising operably linking the DNA sequence to a hybrid promoter comprising the nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98.
- 18. The method of claim 16 wherein operably linking the nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98 or fragment thereof to the promoter confers enhanced expression of operably linked genes in male reproductive tissues.
- 19. The method of claim 18 wherein said male reproductive tissues comprise monocot or dicot male reproductive tissues.
- 20. The method of claim 19 wherein said male reproductive tissues comprise anthers.
- 21. The method of claim 20 wherein said male reproductive tissues comprise wheat anthers.
- 22. The method of claim 16 comprising operably linking a minimal promoter to the nucleic acid sequences selected from the group consisting of SEQ ID NOS:79-98 or fragment, region, or cis element thereof.
- 23. A method of making a transgenic plant comprising introducing into a cell of a plant a DNA construct comprising: (i) a promoter comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98 or a fragment, region or cis element thereof, and, operably linked to the promoter, (ii) a transcribable DNA sequence and (iii) a 3′ non-translated region.
- 24. A method of isolating at least two 5′ regulatory sequences that confer enhanced expression of operably linked genes in male reproductive tissues from a plant comprising:
(i) evaluating a collection of nucleic acid sequences of ESTs derived from at least one cDNA library prepared from a plant cell type of interest; (ii) comparing EST sequences from at least one target plant cDNA library and at least one non-target cDNA libraries of ESTs from a different plant cell type; (iii) subtracting common EST sequences found in both target and non-target libraries; (iv) designing gene specific primers from the remaining EST sequences after said subtraction; and (v) isolating the corresponding 5′ flanking and regulatory sequences from a genomic library prepared from the target plant comprising the use of said primers.
- 25. The method of claim 24 wherein said male reproductive tissues are from monocot or dicot plants.
- 26. The method of claim 25 wherein said male reproductive tissues comprise anthers.
- 27. The method of claim 26 wherein said male reproductive tissues comprise wheat anthers.
RELATED APPLICATION DATA
[0001] This application claims priority to U.S. Provisional Application 60/201,255, filed on May 1, 2000.
Provisional Applications (1)
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Number |
Date |
Country |
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60201255 |
May 2000 |
US |