Claims
- 1. An isolated polynucleotide encoding a functional fertilization-independent endosperm (FIE) polypeptide, wherein the amino acid sequence of the polypeptide is at least 81% identical, based on GAP (GCG Version 10) using default parameters, to SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, or SEQ ID NO:38.
- 2. The isolated polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide is at least 85% identical, based on GAP (GCG Version 10) using default parameters, to SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, or SEQ ID NO:38.
- 3. The isolated polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide is at least 95% identical, based on GAP (GCG Version 10) using default parameters, to SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, or SEQ ID NO:38.
- 4. The isolated polynucleotide of claim 1, wherein the polypeptide comprises SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, or SEQ ID NO:38.
- 5. The isolated polynucleotide of claim 1, wherein the nucleotide sequence is at least 81% identical, based on GAP (GCG Version 10) using default parameters, to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:71, or SEQ ID NO:72.
- 6. The isolated polynucleotide of claim 1, wherein the nucleotide sequence is at least 85% identical, based on GAP (GCG Version 10) using default parameters, to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:71, or SEQ ID NO:72.
- 7. The isolated polynucleotide of claim 1, wherein the nucleotide sequence is at least 95% identical, based on GAP (GCG Version 10) using default parameters, to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:71, or SEQ ID NO:72.
- 8. The isolated polynucleotide of claim 1 comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:71, or SEQ ID NO:72.
- 9. A recombinant expression cassette, comprising a polynucleotide of claim 1 or a fragment thereof, operably linked to a promoter.
- 10. The recombinant expression cassette of claim 9, wherein said polynucleotide is operably linked in antisense orientation to said promoter.
- 11. A host cell transformed with the recombinant expression cassette of claim 9.
- 12. A transgenic plant comprising the recombinant expression cassette of claim 9.
- 13. The transgenic plant of claim 12, wherein the plant is Zea mays.
- 14. A method of modulating seed development in a plant, comprising:
a) transforming a plant cell with a recombinant expression cassette comprising a polynucleotide of claim 1;b) growing said plant cell under conditions which favor plant regeneration; c) regenerating a plant from said transformed plant cell; d) growing said plant under conditions which allow or induce expression of said polynucleotide.
- 15. An isolated promoter capable of driving transcription in a seed-preferred manner, wherein the promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising SEQ ID NOS:73; b) a nucleotide sequence having at least 80% sequence identity to SEQ ID NO:73, wherein the % sequence identity is based on the entire sequence and is determined by GAP (GCG version 10) analysis using default parameters; and c) a nucleotide sequence that hybridizes to SEQ ID NO:73 under highly stringent conditions.
- 16. An expression cassette comprising a promoter of claim 15 and a first nucleotide sequence operably linked to the promoter.
- 17. A transformation vector comprising an expression cassette of claim 16.
- 18. A plant stably transformed with an expression cassette of claim 16.
- 19. The plant of claim 18, wherein the plant is a monocot.
- 20. The plant of claim 19, wherein the monocot is maize, wheat, rice, barley, sorghum, or rye.
- 21. Seed of the plant of claim 18 comprising within their genome said expression cassette.
- 22. A method for selectively expressing a nucleotide sequence in a plant cell, the method comprising transforming a plant cell with a transformation vector of claim 17.
- 23. The method of claim 22 further comprising regenerating a stably transformed plant from the transformed plant cell, wherein expression of the first nucleotide sequence alters the phenotype of the plant seed.
- 24. The method of claim 22, wherein the first nucleotide sequence encodes a gene involved in fatty acid synthesis.
- 25. The method of claim 22, wherein the first nucleotide sequence encodes a gene providing enhanced amino acid content.
- 26. A plant cell stably transformed with an expression cassette of claim 16.
- 27. The plant cell of claim 26, wherein the plant cell is from a monocotyledonous plant.
- 28. The plant cell of claim 27, wherein the plant is maize, wheat, rice, barley, sorghum, or rye.
- 29. An isolated promoter comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence comprising SEQ ID NO:74; (b) a nucleotide sequence having at least 80% sequence identity to SEQ ID NO:74, wherein the % sequence identity is based on the entire sequence and is determined by GAP version 10 analysis using default parameters; and (c) a nucleotide sequence that hybridizes to SEQ ID NO:74 under highly stringent conditions.
- 30. An expression cassette comprising a promoter of claim 29 and a first nucleotide sequence operably linked to the promoter.
- 31. A transformation vector comprising an expression cassette of claim 30.
- 32. A plant stably transformed with an expression cassette of claim 30.
- 33. The plant of claim 32, wherein the plant is a monocot.
- 34. The plant of claim 33, wherein the monocot is maize, wheat, rice, barley, sorghum, or rye.
- 35. Seed of the plant of claim 32 comprising within their genome said expression cassette.
- 36. A method for selectively expressing a nucleotide sequence in a plant cell, the method comprising transforming a plant cell with a transformation vector of claim 31.
- 37. The method of claim 36 further comprising regenerating a stably transformed plant from the transformed plant cell, wherein expression of the first nucleotide sequence alters the phenotype of the plant seed.
- 38. The method of claim 36, wherein the first nucleotide sequence encodes a gene involved in fatty acid synthesis.
- 39. The method of claim 36, wherein the first nucleotide sequence encodes a gene providing enhanced amino acid content.
- 40. A plant cell stably transformed with an expression cassette of claim 30.
- 41. The plant cell of claim 40, wherein the plant cell is from a monocotyledonous plant.
- 42.The plant cell of claim 41, wherein the plant is maize, wheat, rice, barley, sorghum, or rye.
- 43. A method for producing seed in the absence of fertilization, the method comprising modulation of expression, within a plant, of a polynucleotide of claim 1.
- 44. A method for altering endosperm development in seed, the method comprising modulation of expression, within a plant, of a polynucleotide of claim 1.
- 45. A method of plant reproduction comprising embryogenesis from callus tissue derived from fie germplasm, wherein tissue-specific downregulation of a CHD polypeptide stimulates embryogenesis and wherein the fie germplasm comprises endosperm development without fertilization.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PCT/US00/23735 |
Aug 2000 |
US |
|
Parent Case Info
[0001] This application claims the benefit of, and hereby incorporates by reference, international application PCT/US00/23735 filed Aug. 30, 2000 designating the United States, which claims priority to U.S. Provisional Application No. 60/151,575 filed Aug. 31, 1999.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60151575 |
Aug 1999 |
US |