Claims
- 1. Cotyledonary tissues of asexual embryos produced by the following steps:
- (A) proliferation of immature zygotic cacao embryos in a basal medium in the presence of a growth enhancer whereby asexual embryos are initiated upon said zygotic embryos; and
- (B) growing said asexual embryos in vitro in a basal medium;
- (C) harvesting the cotyledonary tissue of said asexual embryos.
- 2. An asexual embryo capable of forming cotyledonous tissue produced by the following steps:
- (A) proliferation of immature zygotic cacao embryos in a basal medium in the presence of a growth enhancer whereby asexual embryos are initiated upon said zygotic embryos; and
- (B) growing said asexual embryos in vitro in a basal medium.
RELATED APPLICATION
This application is a continuation-in-part of our copending U.S. patent application Ser. No. 951,267, filed Oct. 13, 1978 and entitled "A Method of Non-Agricultural Production of Cotyledons (now U.S. Pat. No. 4,204,366)."
This invention relates to a living plant tissue which is produced by in vitro culture of embroys of cacao.
A research report by Brent H. Tisserat, Edward B. Esan and Toshio Murashige entitled "Asexual Embryogenesis in Angiosperms" is the most recent and exhaustive report on asexual embryogenesis in vitro known to applicants.
A section of that report states:
Their Table 2 purports to be a complete survey and does include a few cotyledoneous explants, but there is at least one significant omission, namely, cacao, and a further section of the report, entitled "Morphological Aspects of Asexual Embryogeny in Vitro" makes it apparent that the authors have described the current state of the art solely in terms of its agricultural impact, namely, the aspect of plant reproducibility, and not in any sense have they related embryogenesis to the use to which it is put by applicants.
The authors state their conclusion to be:
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4204366 |
Janick |
May 1980 |
|
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
951267 |
Oct 1978 |
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