Claims
- 1. A nucleic acid molecule for use in cloning and expressing in a plant the nucleic acid sequence encoding a protein influencing flower structure, function and/or its seed and/or fruit development which is selected from the group consisting of
(a) the nucleic acid sequence defined in SEQ ID No. 1, or complementary strand thereof, (b) a nucleic acid sequence encoding a protein or peptide with the amino acid sequence defined in SEQ ID No. 2, or complementary strand thereof, (c) a nucleic acid sequence which hybridizes to the nucleic acid sequence defined a) or b), or a complementary stand thereof, which has a degree of identity of more than 70%, and (d) a nucleic acid sequence which is degenerate, as a result of the genetic code, to the nucleic acid sequence defined in a), b), c), or a complementary strand thereof.
- 2. The nucleic acid molecule of claim 1, which is derived from maize.
- 3. The nucleic molecule of claim 1 which is a DNA or cDNA, or RNA molecule.
- 4. A vector comprising the nucleic acid molecule of claim 1.
- 5. The vector of claim 4, which is a bacterial or viral vector.
- 6. The vector of claim 4, wherein the nucleic acid molecule is operably linked to at least one regulatory element, in particular in an antisense or sense orientation.
- 7. The vector of claim 6, wherein the regulatory element functions at either the 5′ or 3′ end of said nucleic acid.
- 8. The vector of claim 7, wherein the 5′ regulatory element is a promoter, in particular the CaMV 35S promoter or the actin promoter.
- 9. The vector of claim 7, wherein the 3′ regulatory element is a termination and poly A addition sequence, in particular from the NOS gene of Agrobacterium tumefaciens.
- 10. The vector according to claim 4, which furthermore contains T-DNA, in particular either the left, or the right, or both left and right T-DNA borders.
- 11. The vector according to claim 10, wherein the nucleic acid molecule, optionally in conjunction with at least one regulatory element, is located within the T-DNA or adjacent to it.
- 12. A transgenic host cell containing the vector of claim 4 or a cell deriving therefrom.
- 13. The host cell of claim 12, which is a plant, yeast or bacterial cell, in particular a cell from a monocotyledonous or dicotyledonous plant or a cell deriving therefrom.
- 14. A cell culture, preferably a plant cell culture comprising a cell according to claim 12.
- 15. A method of genetically modifying a cell by transforming a cell with a nucleic acid molecule of claim 1 or a vector according to claim 4 wherein the nucleic acid molecule is expressible in the cell.
- 16. The method of claim 15, wherein the cell is a plant, bacterial or yeast cell.
- 17. The method of claim 15, wherein the transformed cell is regenerated into a differentiated plant.
- 18. The method of claim 15 wherein the cell is transformed by transfer of the nucleic acid molecule or vector from a bacterium to the cell.
- 19. The method of claims 15, wherein the cell is transformed by direct uptake of nucleic acid sequences, or by microinjection of nucleic acid sequences, or particle bombardment.
- 20. A method for isolating node, flower and embryo sac genes from a plant, whereby a nucleic acid sequence of claim 1 is used to screen nucleic acid sequences derived from the plant.
- 21. A plant comprising a host cell according to claim 12 or produced according to a method according to claim 15.
- 22. Propagation and harvest material, in particular seeds and plant tissue, comprising a host cell according to 12.
- 23. A method for the production of a genetically modified plant with a modified flower, seed and/or fruit structure, or modified function or development, wherein a plant cell is transformed with a nucleic acid molecule according to claim 1 or a vector according to claim 4 and the transformed cell is regenerated into a plant.
Priority Claims (1)
Number |
Date |
Country |
Kind |
99120842.2 |
Oct 1999 |
EP |
|
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] This application is a continuation to PCT/EP00/10484, which was filed on Oct. 25, 2000 and which claimed priority to EP application 99120842.2, which was filed on Oct. 25, 1999.
Continuations (1)
|
Number |
Date |
Country |
Parent |
PCT/EP00/10484 |
Oct 2000 |
US |
Child |
10105021 |
Mar 2002 |
US |