Claims
- 1. A plant containing a plasmid which comprises a plant species specific patatin promoter operably linked to a DNA sequence encoding a polypeptide which comprises a signal peptide fused to invertase wherein the signal peptide is functionally capable of directing the translocation of the invertase to a desired location in a plant cell.
- 2. A plant according to claim 1, wherein said desired location is a plant cell apoplast.
- 3. A plant according to claim 2, wherein the plant is a crop plant.
- 4. A plant according to claim 3, wherein the crop plant is selected from the group consisting of tobacco, potato, tomato, sugar beet, soya bean, wheat, barley, corn, oat, rice and sugar cane plants.
- 5. A plant according to claim 2, wherein said plasmid is p33-CW-INV deposited as DSM 5787.
- 6. A plant according to claim 5, wherein the plant is a crop plant.
- 7. A plant according to claim 6, wherein the crop plant is selected from the group consisting of tobacco, potato, tomato, sugar beet, soya bean, wheat, barley, corn, oat, rice and sugar cane plants.
- 8. A plant according to claim 1, wherein the plant is a monocot.
- 9. A plant according to claim 1, wherein the plant is a dicot.
- 10. A plant according to claim 1, wherein said desired location is a plant cell vacuole.
- 11. A plant according to claim 10, wherein the plant is a crop plant.
- 12. A plant according to claim 11, wherein the crop plant is selected from the group consisting of tobacco, potato, tomato, sugar beet, soya bean, wheat, barley, corn, oat, rice and sugar cane plants.
- 13. A plant according to claim 11, wherein said plasmid is p33-CW-INV deposited as DSM 5787.
- 14. A method for the preparation of a plant with an increased dry weight of a sink organ comprising transforming plants with plasmid p33-CW-INV deposited as DSM 5787.
- 15. The method according to claim 14, wherein the sink organ is selected from the group consisting of seeds, fruits, roots and tubers.
- 16. A plasmid containing a DNA sequence, wherein the DNA sequence directs the translocation of invertase to a vacuole of a plant cell, said DNA sequence is operably linked to a DNA sequence encoding invertase, wherein the DNA sequence directing the translocation of invertase to a vacuole of a plant cell comprises nucleotide position +707 to +1895 of patatin gene pgT5 from Solanum tuberosum (SEQ. ID No. 2) or sequences which hybridize to the DNA sequence directing the translocation under stringent conditions.
- 17. Plasmid p35S-V-INV deposited as DSM 6142.
- 18. A plant stably transformed with a plasmid according to claim 17.
- 19. A plant according to claim 18, wherein the plant is a crop plant.
- 20. A plant according to claim 19, wherein the crop plant is selected from the group consisting of tobacco, potato, tomato, sugar beet, soya bean, wheat, barley, corn, oat, rice and sugar cane plants.
- 21. A plant cell transformed with a plasmid which comprises a plant species specific patatin promoter operably linked to a DNA sequence encoding a polypeptide which comprises a signal peptide fused to invertase wherein the signal peptide is functionally capable of directing the translocation of the invertase to a desired location in a plant cell.
- 22. A plant cell according to claim 21, wherein said desired location is a plant cell apoplast.
- 23. A plant cell according to claim 22, wherein the plant cell is obtained from a crop plant.
- 24. A plant cell according to claim 22, wherein the plant cell is obtained from a monocot.
- 25. A plant cell according to claim 22, wherein the plant cell is obtained from a dicot.
- 26. A plant cell according to claim 22, wherein said plasmid is p33-CW-INV deposited as DSM 5787.
- 27. A plant cell according to claim 21, wherein said desired location is a plant cell vacuole.
- 28. A plant cell according to claim 27, wherein said plasmid is p33-CW-INV deposited as DSM 5787.
- 29. A plant cell according to claim 27, wherein the plant cell is obtained from a crop plant.
- 30. A plant cell according to claim 27, wherein the plant cell is obtained from a monocot.
- 31. A plant cell according to claim 27, wherein the plant cell is obtained from a dicot.
- 32. A method for the production of transgenic plants, comprising the following steps:
- a) producing an expression cassette having the following sequences:
- i) a class I patatin promoter, operably linked to,
- ii) a DNA sequence encoding invertase, fused to
- iii) a DNA sequence of a signal peptide, wherein the signal peptide is functionally capable of directing the translocation of the invertase to a desired location in a plant cell;
- b) transferring the expression cassette into plant cells, thereby producing transformed plant cells; and
- c) regenerating whole, intact transgenic plants from the transformed cells.
- 33. A method according to claim 32, wherein said desired location is a plant cell vacuole.
- 34. A method according to claim 33, wherein said plants are monocots.
- 35. A method according to claim 33, wherein said plants are dicots.
- 36. A method according to claim 32, wherein said desired location is a plant cell apoplast.
- 37. A method according to claim 36, wherein said plants are monocots.
- 38. A method according to claim 36, wherein said plants are dicots.
Priority Claims (1)
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40 04 800 |
Feb 1990 |
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Parent Case Info
This is a continuation-in-part of application Ser. No. 08/156,876 filed Nov. 23, 1993 now U.S. Pat. No. 5,436,394 which was a continuation of application Ser. No. 07/653,689 filed Feb. 11, 1991, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5436394 |
Willmitzer et al. |
Jul 1995 |
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Non-Patent Literature Citations (3)
Entry |
Wenzler et al. Sucrose-regulated expression of a chimeric potato tuber gene in leaves of Transgenic tobacco plants. Plant Moleclar Biology 13: 347-354, 1989. |
Mignery et al. Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin. Nucleic Acids Research, vol. 12, No. 21, 1984. |
Mignery et al. Molecular characterization of the patatin multigene family of potato, Gene 62 (1988) 27-44, 1988. |
Continuations (1)
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Date |
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Parent |
653689 |
Feb 1991 |
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Continuation in Parts (1)
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Number |
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156876 |
Nov 1993 |
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