POC Biosensor for Periodontitis

Information

  • Research Project
  • 10244668
  • ApplicationId
    10244668
  • Core Project Number
    R21DE028715
  • Full Project Number
    3R21DE028715-01A1S1
  • Serial Number
    028715
  • FOA Number
    PA-19-053
  • Sub Project Id
  • Project Start Date
    9/1/2020 - 3 years ago
  • Project End Date
    8/31/2021 - 2 years ago
  • Program Officer Name
    CHANDER, PREETHI
  • Budget Start Date
    9/1/2020 - 3 years ago
  • Budget End Date
    5/31/2021 - 3 years ago
  • Fiscal Year
    2020
  • Support Year
    01
  • Suffix
    A1S1
  • Award Notice Date
    8/21/2020 - 3 years ago
Organizations

POC Biosensor for Periodontitis

For nearly a century, practitioners have typically relied on periodontal probing to diagnose periodontitis. Based on a strong positive correlation between pocket depth and severity of periodontitis, the primary purpose of periodontal probing is to measure pocket depths around a tooth in order to establish the state of health of the periodontium. However, periodontal probing is a time-consuming chairside procedure and has little predictive value in determining current disease activity, prognosis of future progression, or healing in response to treatment, essentially because scant quantitative biological information gained from measuring pocket depth. In this study, we will use ?lab-on-a-chip? device, SMARTChip® Biosensor, to measure extracellular ATP (eATP) and extracellular Adenosine (eADO) in GCF. More specifically, GCF collected from patients using a paper point will be diluted into PBS and immediately applied to SMARTChip at chairside. Unlike other subjective POC devices, enzyme-based amperometric SMARTChip® can quantify eATP and eADO in GCF. Recent studies have demonstrated that eATP released from damaged cells or activated neutrophils is causal for inflammatory responses by binding with a series of purinergic P2X and P2Y receptors. This eATP induces proinflammatory factors, such as TNF-? and IL-1?, from innate immune cells, suggesting that eATP is an early- response inflammatory mediator. Meanwhile, ectonucleotidases, CD39 and CD73, produced by Treg and Breg cells, as well as endothelial cells convert ATP to anti-inflammatory eADO. We have learned that eADO, by ligation with its specific ADO receptor, suppresses inflammation by down-modulating TNF-? and IL-1? expressions, and promotes the production of BMP2. These lines of evidence suggest that eATP is associated with inflammation, but that eADO may function as an anti-inflammatory and pro-osteogenesis factor in periodontitis. Our hypothesis holds that SMARTChip® measurements of eATP/eADO in GCF will serve to determine inflammatory status in periodontitis lesion and act as a predictor of bone regenerative responses. In order to test this hypothesis we will validate POC SMARTChip® measurements of eATP and eADO in GCF for the diagnosis periodontitis and prediction of treatment-related bone regenerative activities. Three cohorts will be recruited for this study; A) control healthy subjects, B) gingivitis patients and C) periodontitis patients. Conventional periodontal measurements (pocket depth, clinical attachment loss, bleeding on probing and gingival index) and standardized periapical radiograph will be performed. Then, GCF will be collected by a PerioPaper (GCF Collection Strip) at the deepest periodontal pocket or gingival crevice from each quadrant, and the concentrations of eATP and eADO will be monitored at chairside using a SMARTChip®. A panel of biomarkers will be monitored in GCF using Luminex® Multiplex which will be compared to eATP/eADO for correlation with the clinical measurements of pocket depth and clinical attachment loss as well as treatment- related bone regenerative activities in the study subjects.

IC Name
NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
  • Activity
    R21
  • Administering IC
    DE
  • Application Type
    3
  • Direct Cost Amount
    19134
  • Indirect Cost Amount
    9950
  • Total Cost
    29084
  • Sub Project Total Cost
  • ARRA Funded
    False
  • CFDA Code
    121
  • Ed Inst. Type
    SCHOOLS OF DENTISTRY/ORAL HYGN
  • Funding ICs
    NIDCR:29084\
  • Funding Mechanism
    Non-SBIR/STTR RPGs
  • Study Section
    ODCS
  • Study Section Name
    Oral, Dental and Craniofacial Sciences Study Section
  • Organization Name
    NOVA SOUTHEASTERN UNIVERSITY
  • Organization Department
    DENTISTRY
  • Organization DUNS
    002971240
  • Organization City
    Fort Lauderdale
  • Organization State
    FL
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    333147796
  • Organization District
    UNITED STATES