Claims
- 1. A process for regulating COX2 gene expression in a cell containing the COX2 gene the process comprising introducing a polyamide into the cell, the polyamide 5 comprising N-methyl pyrrole (Py) and N-methyl imidazole (IM) to provide specific binding to DNA at a COX2 gene promoter target site in a cell, wherein said polyamide binds to the COX2 gene promoter target site and regulates transcription of the COX2 gene.
- 2. The process of claim 1, wherein the polyamide binds sequences which partially or completely overlaps a transcription factor binding site for the COX2 gene.
- 3. The process of claim 2, wherein the transcription factor binding site is selected from the group consisting of CCRE, NFkB, Ets-1, LEF-1, and TATA box.
- 4. The process of claim 3 wherein the polyamide enhances the transcription of the COX2 gene.
- 5. The process of claim 4, wherein the transcription factor binding sites are selected from the group consisting of NFkB and LEF-1.
- 6. The process of claim 3, wherein the polyamide suppresses the transcription of the COX2 gene.
- 7. The process of claim 6, wherein the transcription factor binding sites are selected from the group consisting of CCRE, Ets-1, and TATA box.
- 8. The process of claim 3, wherein at least two different polyamides bind to one or more transcription factor binding site.
- 9. The process of claim 1, wherein the polyamide binds to the DNA singly or in pairs.
- 10. The process of claim 1, wherein the polyamide forms a hairpin turn.
- 11. A polyamide compound for regulating COX2 gene expression comprising N-methyl pyrrole (Py) and N-methyl imidazole (IM), said polyamide compound specifically binding to a COX2 gene promoter region of DNA.
- 12. The polyamide compound of claim 11 wherein the polyamides bind sequences which partially or completely overlaps a transcription factor binding site for the COX2 gene.
- 13. The polyamide compound of claim 12, wherein the polyamide compound binds to the transcription factor binding site is selected from the group consisting of CCRE, NFkB, Ets-1, LEF-1, and TATA box.
- 14. The polyamide compound of claim 13, wherein the transcription factor binding site is CCRE and the polyamide compound is Im-Py-Py-Im-γ-Py-Im-Py-Py-β-Dp.
- 15. The polyamide compound of claim 13, wherein the transcription factor binding site is Ets-1 and the polyamide compound is selected from the group consisting of Im-Im-Py-Py-γ-Py-Im-Py-Py-β-Dp, Im-Im-β-Py-γ-Py-Im-Py-Py-β-Dp, Im-Im-Py-Py-γ-Py-Im-β-Py-β-Dp, Im-Im-Py-Im-γ-Py-Py-β-Py-β-Dp, Im-Im-β-Im-γ-Py-Py-Py-Py-β-Dp, and Im-β-Py-Py-γ-Py-Im-Im-Py-β-Dp.
- 16. The polyamide compound of claim 13, wherein the transcription factor binding site is TATA box and the polyamide compound is Im-Py-Py-Py-Im-γ-Py-Py-Im-Py-Py-β-Dp.
- 17. The polyamide compound of claim 13, wherein the transcription factor binding site is NFkB and the polyamide compound is selected from the group consisting of Im-Im-Im-Im-γ-Py-Py-β-Py-β-Dp, Im-Im-Im-β-Py-γ-Py-Py-Py-Py-Py-β-Dp, Im-Im-Im-Py-Py-γ-Py-β-Py-Py-Py-β-Dp, Im-Im-Im-Py-γ-Py-Py-Py-Py-β-Dp, Im-Im-Im-Py-γ-Py-β-Py-Py-β-Dp, Im-Im-Im-Py-γ-Py-Py-β-Py-β-Dp, and Im-Im-Im-Im-Im-γ-Py-β-Py-Py-Py-β-Dp.
- 18. The polyamide compound of claim 13, wherein the transcription factor binding site is LEF-1 and the polyamide compound is selected from the group consisting of Im-β-Im-Py-γ-Im-β-Im-Py-β-Dp, Im-Py-Im-Py-γ-Im-Py-Im-Py-β-Dp, Im-Py-Py-Py-Im-γ-Py-Im-Im-Im-Py-β-Dp, Im-β-Py-Py-Im-γ-Py-Im-Im-Im-Py-β-Dp, Im-Py-Py-β-Im-Py-Im-γ-Py-Im-Py-β-Im-Py-Py-β-Dp, Im-Py-Py-γ-Im-Py-Py-β-Dp, and Im-Py-Py-Im-Py-γ-Py-Py-Py-Py-Py-β-Dp.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application claims priority from U.S. application Serial No. 60/419,405, filed Oct. 18, 2002 and U.S. application Serial No. 60/344,859, filed Nov. 7, 2001.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60419405 |
Oct 2002 |
US |
|
60344859 |
Nov 2001 |
US |