Patent Grant
7504422
Chemokines are a family of cytokines that regulate the adhesion and transendothelial migration of leukocytes during an immune or inflammatory reaction (Mackay C. R., Nat. Immunol., (2001) 2:95; Olson et al., Am. J. Physiol. Regul. Integr. Comp. Physiol., (2002) 283:R7). Chemokines also regulate T cells and B cells trafficking and homing, and contribute to the development of lymphopoietic and hematopoietic systems (Ajuebor et al., Biochem. Pharmacol., (2002) 63:1191). Approximately 50 chemokines have been identified in humans. They can be classified into 4 subfamilies, i.e., CXC, CX3C, CC, and C chemokines, based on the positions of the conserved cysteine residues at the N-terminal (Onuffer et al., Trends Pharmacol Sci., (2002) 23:459). The biological functions of chemokines are mediated by their binding and activation of G protein-coupled receptors (GPCRs) on the cell surface. Take CXCR4 receptor for example, it can be activated by Stromal-derived factor-1 or SDF-1, a member of CXC chemokines.
SDF-1 was originally cloned from bone marrow stromal cell lines and found to act as a growth factor for progenitor B cells (Nishikawa et al., Eur. J. Immunol., (1988) 18:1767). SDF-1 also induces bone marrow colonization of hematopoietic precursor cells during embryogenesis (Bleul et al., J. Exp. Med., (1996) 184:1101). The physiological function of SDF-1 is mediated by CXCR4 receptor. Mice lacking SDF-1 or CXCR4 receptor show lethal abnormality in bone marrow myelopoiesis, B cell lymphopoiesis, and cerebellar development (Nagasawa et al., Nature, (1996) 382:635; Ma et al., Proc. Natl. Acad. Sci., (1998) 95:9448; Zou et al., Nature (1998) 393:595; Lu et al., Proc. Natl. Acad. Sci. (2002) 99:7090). CXCR4 receptor is expressed broadly in a variety of tissues, particularly in immune and central nervous systems, and has been described as the major co-receptor for HIV-1/2 on T lymphocytes. Although initial interest in CXCR4 antagonism focused on its potential application to AIDS treatment (Bleul et al., Nature (1996) 382:829), it is now becoming clear that CXCR4 receptor and SDF-1 are also involved in other pathological conditions such as rheumatoid arthritis, asthma, and tumor metastases (Buckley et al., J. Immunol., (2000) 165:3423). CXCR4 receptor and SDF-1 are also found widely expressed in many tissues during embryonic development. Further, the CXCR4/SDF-1 pathway has been shown to be critically involved in the regeneration of several tissue injury models. Specifically, it has been found that the SDF-1 level is elevated at an injured site and CXCR4-positive cells actively participate in the tissue regenerating process.
This invention is based on the unexpected discovery that the polyamine compounds described below, either alone or in combination of granulocyte-colony stimulating factor (G-CSF), promote step/progenitor cell mobilization via blocking of the interaction between chemokine receptors (e.g., CXCR3 and CXCR4) and their ligands (e.g., SDF-1).
In one aspect, this invention features polyamine compounds of the formula:
In the above formula, X is —CH2—, —C2H4—, —C3H6—, —CH2—CH═CH—, —CH═CH—CH2—, —C(O)—, —SO2—, or deleted; Y is aryl, heteroaryl, C3-C8 cycloalkyl, C5-C8 cycloalkenyl, C3-C8 heterocycloalkyl, or C5-C8 heterocycloalkenyl; each of Z1 and Z2, independently, is —CH2—, —C2H4—, —C3H6—, —CH═CH—, —CH═N—, —CH═N—NR—, —S—, —O—, —NR—, —C(O)—, or —SO2—; R1 is H, C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl, C3-C8 cycloalkyl, C5-C8 cycloalkenyl, C3-C8 heterocycloalkyl; C5-C8 heterocycloalkenyl, aryl, or heteroaryl; R2 is -A1-B1-D1-E1; R3 is -A2-B2-D2-E2, deleted, or, together with R4, is C4-C20 cycloalkyl, C4-C20 cycloalkenyl, C4-C20 heterocycloalkyl, or C4-C20 heterocycloalkenyl; provided that if R3 is deleted, -Z2-N— is —CH═N—; and R4 is -A3-B3-D3-E3 or, together with R3, is C4-C20 cycloalkyl, C4-C20 cycloalkenyl, C4-C20 heterocycloalkyl, or C4-C20 heterocycloalkenyl. Each of A1, A2, and A3, independently, is —CH2—, —C2H4—, —C3H6—, —C4H8—, —C5H10, —CH2C(O)—, —C(O)CH2—, —CH2SO2—, —SO2CH2—, —CH2—CH═CH—, —CH═CH—CH2—, —CH(CH2OR)—, —CH(CH2CH2OR)—, —CH(COOR)—, —CH(CH2COOR)—, —CH(C(O)NR2)—, or deleted. Each of B1, B2, and B3, independently, is —NR—, —CH2—, or deleted. Each of D1, D2, and D3, independently, is —CH2—, —C2H4—, —C3H6—, —CH2—CH═CH—, —CH═CH—CH2—, —C(O)—, —SO2—, —C(O)—NR—, —C(S)—NR—, —NR—C(O)—, —NR—C(S)—, —CH(OR)—, —CH(CH2OR)—, —CH(CH2CH2OR)—, —CH(COOR)—, 1,1-cyclopropylene, or deleted. Each of E1, E2, and E3, independently, is H, C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl, C3-C8 cycloalkyl, C5-C8 cycloalkenyl, C3-C8 heterocycloalkyl, C5-C8 heterocycloalkenyl, aryl, or heteroaryl. Each R, independently, is H or C1-C10 alkyl.
Referring to the above formula, a subset of the just-described compounds are those in which X is —CH2— or —CH(CH3)—, Y is phenyl or 4,4′-biphenyl, Z1 is —CH2— or —SO2—, Z2 is —CH2— or —SO2—, or R3 is deleted.
The term “alkyl” refers to a saturated, linear or branched, non-aromatic hydrocarbon moiety, such as CH3, —CH2—, or branched C3H7. Referring to the above formula, —C2H4— and —C3H6— can be either linear or branched. The term “alkenyl” refers to a linear or branched, non-aromatic hydrocarbon moiety having at least one double bond, such as —CH═CH2 or —CH═CH—. The term “alkynyl” refers to a linear or branched, non-aromatic hydrocarbon moiety having at least one triple bond, such as —C≡CH or —C≡C—. The term “cycloalkyl” refers to a saturated non-aromatic cyclic hydrocarbon moiety. The term “cycloalkenyl” refers to a non-aromatic cyclic hydrocarbon moiety having at least one double bond in the ring. The term “heterocycloalkyl” refers to a saturated non-aromatic cyclic moiety having at least one ring heteroatom (e.g., O, N, and S). The term “heterocycloalkenyl” refers to a non-aromatic cyclic moiety having at least one ring heteroatom and at least one double bond in the ring. The term “aryl” refers to a hydrocarbon moiety having at least one aromatic ring. Examples of an aryl moiety include phenyl, phenylene, biphenyl, naphthyl, naphthylene, pyrenyl, anthryl, and phenanthryl. The term “heteroaryl” refers to a moiety having at least one aromatic ring which contains at least one heteroatom. Examples of a heteroaryl moiety include furyl, furylene, fluorenyl, pyrrolyl, thienyl, oxazolyl, imidazolyl, thiazolyl, pyridyl, pyrimidinyl, quinazolinyl, and indolyl.
Alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, aryl, and heteroaryl mentioned herein include both substituted and unsubstituted moieties. Examples of substituents for cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, aryl, and heteroaryl include C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl, C3-C8 cycloalkyl, C5-C8 cycloalkenyl, C1-C10 alkoxy, aryl, aryloxy, heteroaryl, heteroaryloxy, amino, C1-C10 alkylamino, C1-C20 dialkylamino, arylamino, diarylamino, heteroarylamino, diheteroarylamino, C1-C10 alkylsulfonyl, arylsulfonyl, heteroarylsulfonyl, C1-C10 alkylsulfonamide, arylsulfonamide, heteroarylsulfonamide, hydroxyl, halogen, mercapto, C1-C10 alkylmercapto, arylmercapto, cyano, nitro, acyl, acyloxy, carboxyl, amido, carbamoyl, and carboxylic ester. Examples of substituents for alkyl, alkenyl, and alkynyl include all of the above substitutents except C1-C10 alkyl, C2-C10 alkenyl, and C2-C10 alkynyl. Cycloalkyl, heterocycloalkyl, aryl, and heteroaryl also include fused groups.
In another aspect, this invention features polyamine compounds of the same formula shown above. Referring to this formula, the same groups as those described above are assigned to each variable except that X is —CH2—, —C2H4—, —C3H6—, —CH2—CH═CH—, —CH═CH—CH2—, —SO2—, or deleted; Y is aryl, heteroaryl, C3-C8 cycloalkyl, C5-C8 cycloalkenyl, C3-C8 heterocycloalkyl, C5-C8 heterocycloalkenyl, or deleted; each of D1, D2, and D3, independently, is —CH2—, —C2H4—, —C3H6—, —CH2—CH═CH—, —CH═CH—CH2—, —SO2—, —C(O)—NR—, —C(S)—NR—, —NR—C(O)—, —NR—C(S)—, —CH(OR)—, —CH(CH2OR)—, —CH(CH2CH2OR)—, —CH(COOR)—, 1,1-cyclopropylene, or deleted; and E1 is H, C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl, C3-C8 cycloalkyl, C5-C8 cycloalkenyl, C3-C8 heterocycloalkyl, C5-C8 heterocycloalkenyl, aryl, 5-membered heteroaryl, fused heteroaryl, substituted 6-membered heteroaryl, unsubstituted pryanyl, unsubstituted pyrazinyl, unsubstituted pyrimidinyl, or unsubstituted pyridazinyl.
Referring to the above formula, a subset of the just-described compounds are those in which X is —CH2— or —CH(CH3)—, Y is deleted, Z1 is —CH2—, or Z2 is —CH2—.
In still another aspect, this invention features a method for treating an inflammatory or immune disease, a developmental or degenerative disease, or a tissue injury. The method includes administering to a subject in need thereof an effective amount of one or more compounds of the same formula shown above. Referring to this formula, X is —CH2—, —C2H4—, —C3H6—, —CH2—CH═CH—, —CH═CH—CH2—, —C(O)—, —SO2—, or deleted; Y is aryl, heteroaryl, C3-C8 cycloalkyl, C5-C8 cycloalkenyl, C3-C8 heterocycloalkyl, C5-C8 heterocycloalkenyl, or deleted; each of Z1 and Z2, independently, is —CH2—, —C2H4—, —C3H6—, —CH═CH—, —CH—N—, —CH═N—NR—, —S—, —O—, —NR—, —C(O)—, or —SO2—; R1 is H, C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl, C3-C8 cycloalkyl, C5-C8 cycloalkenyl, C3-C8 heterocycloalkyl; C5-C8 heterocycloalkenyl, aryl, or heteroaryl; R2 is -A1-B1-D1-E1; R3 is -A2-B2-D2-E2, deleted, or, together with R4, is C4-C20 cycloalkyl, C4-C20 cycloalkenyl, C4-C20 heterocycloalkyl, or C4-C20 heterocycloalkenyl; provided that if R3 is deleted, -Z2-N— is —CH═N—; and R4 is -A3-B3-D3-E3 or, together with R3, is C4-C20 cycloalkyl, C4-C20 cycloalkenyl, C4-C20 heterocycloalkyl, or C4-C20 heterocycloalkenyl. Each of A1, A2, and A3, independently, is —CH2—, —C2H4—, —C3H6—, —C4H8—, —C5H10—, —CH2C(O)—, —C(O)CH2—, —CH2SO2—, —SO2CH2—, —CH2—CH═CH—, —CH═CH—CH2—, —CH(CH2OR)—, —CH(CH2CH2OR)—, —CH(COOR)—, —CH(CH2COOR)—, —CH(C(O)NR2)—, or deleted. Each of B1, B2, and B3, independently, is —NR—, —CH2—, or deleted. Each of D1, D2, and D3, independently, is —CH2—, —C2H4—, —C3H6—, —CH2—CH═CH—, —CH═CH—CH2—, —C(O)—, —SO2—, —C(O)—NR—, —C(S)—NR—, —NR—C(O)—, —NR—C(S)—, —CH(OR)—, —CH(CH2OR)—, —CH(CH2CH2OR)—, —CH(COOR)—, 1,1-cyclopropylene, or deleted. Each of E1, E2, and E3, independently, is H, C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl, C3-C8 cycloalkyl, C5-C8 cycloalkenyl, C3-C8 heterocycloalkyl, C5-C8 heterocycloalkenyl, aryl, or heteroaryl. Each R, independently, is H or C1-C10 alkyl.
For example, one can administer to a subject having an above-described disease a compound of the above formula, in which X is —CH2— or —CH(CH3)—, Y is phenyl, 4,4′-biphenyl, or deleted, Z1 is —CH2— or —SO2—, Z2 is —CH2— or —SO2—, or R3 is deleted. In these compounds, E3 can be phenyl optionally substituted with halo or OR′, benzoimidazole optionally substituted with OR′, indole optionally substituted with C1-C10 alkyl, cyclopentyl fused with phenyl, piperidinyl substituted with aryl or OR′, piperidinyl fused with C1-C10 alkyl substituted indole, or pyrrolidinyl substituted with C1-C10 alkyl, in which R′ is H or C1-C10 alkyl.
“Treatment” refers to administering one or more polyamine compounds to a subject, who has a disease described herein, a symptom of such a disease, or a predisposition toward such a disease, with the purpose to confer a therapeutic effect, e.g., to cure, relieve, alter, affect, ameliorate, or prevent the above-described disease, the symptom of it, or the predisposition toward it.
An inflammatory disease is characterized by a local or systemic, acute or chronic inflammation. Examples include inflammatory retinopathy (e.g., diabetic retinopathy), dermatoses (e.g., dermatitis, eczema, atopic dermatitis, allergic contact dermatitis, urticaria, necrotizing vasculitis, cutaneous vasculitis, hypersensitivity vasculitis, eosinophilic myositis, polymyositis, dermatomyositis, and eosinophilic fascitis), inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), hypersensitivity lung diseases (e.g., hypersensitivity pneumonitis, eosinophilic pneumonia, delayed-type hypersensitivity, interstitial lung disease or ILD, idiopathic pulmonary fibrosis, and ILD associated with rheumatoid arthritis), asthma, and allergic rhinitis. When the polyamine compounds described above are used to treat retinopathy, the compounds are preferably administered to an eye of the subject. For example, a polyamine compound in a solution can be injected into an eye (e.g., into its vitreal space). As another example, the compound can be topically administered to an eye, such as dropped into the eye in the form of an eye drop or applied to an area around the eye in the form of an ointment.
An immune disease is characterized by a hyper- or hypo-reaction of the immune system. Examples include autoimmune diseases (e.g., rheumatoid arthritis, psoriatic arthritis, systemic lupus erythematosus, myasthenia gravis, juvenile onset diabetes, glomerulonephritis, autoimmune thyroiditis, ankylosing spondylitis, systemic sclerosis, and multiple sclerosis), acute and chronic inflammatory diseases (e.g., systemic anaphylaxia or hypersensitivity responses, drug allergies, insect sting allergies, graft rejection, including allograft rejection, and graft-versus-host disease), Sjogren's syndrome, human immunodeficiency virus infection, cancer (e.g., brain, breast, prostate, colon, kidney, ovary, thyroid, lung, and haematopoietic cancer), and tumor metastasis.
Developmental diseases are growth or differentiation related disorders that lead to loss-of-function or gain-of-function. Degenerative diseases generally refer to change of a tissue to a lower or less functional form. Examples of a developmental or degenerative disease include spinal muscular atrophy, Duchenne muscular dystrophy, Parkinson's disease, and Alzheimer's disease. Tissue injuries can be caused by oxidative stress (e.g., ischemia-reperfusion in stroke or myocardial infarction), complement activation, graft rejection, chemicals (e.g., alcohol-induced liver damage or mucosal tissue injuries in cancer therapy), viral infection (e.g., glomerular injuries associated with hepatitis C infection), and mechanical forces (e.g., sports injury).
Examples of tissue injuries include brain injury, heart injury, liver damage, skeletal muscle injury, kidney damage, pancreatic injury, lung injury, skin injury, and gastrointestinal tract injury. In yet another aspect, this invention features a method of treating retinopathy by administering to an eye of a subject in need thereof an effective amount of one or more compounds of the formula shown above. Retinopathy, a non-inflammatory disease of the retina, includes diabetic retinopathy, proliferative retinopathy, age-related macular degeneration, macular edema, corneal neovascularization, and iris neovascularization. The one or more compounds can be applied to an eye, i.e., directly applied to an eye or applied near an eye and let them diffuse to the eye.
In addition, the polyamine compounds can be used to treat diseases associated with edema or angiogenesis.
Also within the scope of this invention is a method for repairing tissue damage with one or more of the compounds described above. Tissue damage refers to a tissue or organ injury caused by loss of a certain type or types of cells (e.g., islet cells, neural lineage cells, hepatic cells, muscle cells, blood cells, and epithelial cells), resulting in dysfunction of the tissue or organ. The term “repair tissue damage” refers to full or partial restoration of the function of the tissue/organ where damage has taken place. Tissue damage can be caused by various diseases, including degenerative disease (e.g., Alzheimer's disease, Parkison's disease, osteoarthritis, and osteoporosis), tissue ischemia (e.g., cardiac ischemia, limb ischemia, nerve ischemia, liver ischemia, kidney ischemia, pancreatic ischemia, lung ischemia, and intestine ischemia), and autoimmune disease (e.g., type I diabetes, systemic lupus erythematosus, Sjogren's syndrome, Hashimoto's thyroiditis, Graves' disease, and rheumatoid arthritis.) These compounds can also be used to treat the just-mentioned underlining diseases themselves.
A subject in need of treatment of an above-described disease or in need of repair of tissue damage can also be concurrently administered with a polyamine compound described above and one or more other therapeutic agents. Examples of such a therapeutic agent include a G-CSF growth factor, a steroidal or a non-steroidal anti-inflammatory drug, a COX2 inhibitor, a leukotriene receptor inhibitor, a prostaglandin modulator, a TNF modulator, and an immunosuppressive agent (e.g., cyclosporine A). The term “concurrently administered” refers to administering a polyamine compound and one or more other therapeutic agents at the same time or at different times during the period of treatment.
In a further aspect, this invention features a method for enhancing migration of bone marrow-derived cells to blood. The method includes administering to a subject in need thereof an effective amount of one or more compounds of the same formula shown above. Referring to this formula, the same groups as those described above are assigned to each variable. The term “bone marrow-derived cells” refers to cells originating from bone marrow. Examples of bone marrow-derived cells include, but are not limited to, CD34+ cells and CD133+ cells.
Also within the scope of this invention is a pharmaceutical composition that contains an effective amount of at least one of the above-mentioned polyamine compounds and a pharmaceutically acceptable carrier.
The polyamine compounds described above include the compounds themselves, as well as their salts, prodrugs, and solvates, if applicable. A salt, for example, can be formed between an anion and a positively charged group (e.g., amino) on a polyamine compound. Suitable anions include chloride, bromide, iodide, sulfate, nitrate, phosphate, citrate, methanesulfonate, trifluoroacetate, and acetate. Likewise, a salt can also be formed between a cation and a negatively charged group (e.g., carboxylate) on a polyamine compound. Suitable cations include sodium ion, potassium ion, magnesium ion, calcium ion, and an ammonium cation such as tetramethylammonium ion. The polyamine compounds also include those salts containing quaternary nitrogen atoms. Examples of prodrugs include esters and other pharmaceutically acceptable derivatives, which, upon administration to a subject, are capable of providing active polyamine compounds. A solvate refers to a complex formed between an active polyamine compound and a pharmaceutically acceptable solvent. Examples of pharmaceutically acceptable solvents include water, ethanol, isopropanol, ethyl acetate, acetic acid, and ethanolamine.
Also within the scope of this invention is a composition containing one or more of the polyamine compounds described above for use in treating an above-described disease, and the use of such a composition for the manufacture of a medicament for the just-mentioned treatment.
The details of one or more embodiments of the invention are set forth in the description below. Other features, objects, and advantages of the invention will be apparent from the description and from the claims.
Shown below are exemplary compounds, compounds 1-143, of this invention:
The polyamine compounds described in the summary section above can be prepared by methods well known in the art, including the synthetic routes disclosed herein.
For example, compound 1-11 can be respectively prepared by reacting tris(2-aminoethyl)amine with three equivalent amounts of a corresponding aldehyde, and followed by a reduction reaction using sodium borohydride.
In another example, one can react 1,4-dibromoxylene with two equivalent amounts of bis(t-butoxycarbonylaminoethyl)amine. The reaction mixture is subsequently treated with hydrochloric acid to give an intermediate, 1,4-di[bis(2-aminoethyl)aminomethyl]benzene. This intermediate can react with four equivalent amounts of corresponding aldehyde compounds and sodium borohydride to give compounds 12-14. Alternatively, this intermediate can react with two equivalent amounts of pyridine-2-carbaldehyde, and then with sodium borohydride to give compound 15. One can also react 1,4-dibromoxylene with one equivalent amount of bis(t-butoxycarbonylaminoethyl)amine and one equivalent amount of other amine compounds. Compounds 56-57, 65, 66, 68, 80, 86, 91, 93-95, 106-109, 141, and 142 can be prepared through this synthetic route, followed by sequential treatments with hydrochloric acid, two equivalent amounts of a corresponding aldehyde, and sodium borohydride. In a similar manner, compounds 49 can be prepared by reacting 1,4-dibromoxylene with one equivalent amount of tri-t-butoxycarbonyl protected cyclam and one equivalent amount of bis(2-pyridiyliminoethyl)amine, followed by a reduction reaction using sodium borohydride.
In another example, compounds 16-48, 58-64, 69-79, 81-85, 87-90, 92, 96-105, 115, 121, 122, and 125-141 can be synthesized using the following synthetic route. One can react 4-cyanobenzylbromide with one equivalent amount of bis(t-butoxycarbonylaminoethyl)amine and then hydrochloric acid to give 4-[bis(2-aminoethyl)amino-methyl]-benzonitrile. This benzonitrile can then be treated sequentially with two equivalent amounts of an aldehyde, sodium borohydride, and diisobutylaluminum to form 4-[bis(2-substituted-aminoethyl)amino-methyl]-benzaldehyde. The just-mentioned compounds can then be prepared by treating this benzaldehyde sequentially with one equivalent amount of a corresponding amine, sodium borohydride, and hydrochloric acid. Compounds 110, 111, 116, 117, 120, 123, and 124 can be prepared in a similar manner except that the benzonitrile is treated with one equivalent amount of an aldehyde and one equivalent amount of a ketone.
In another example, one can react 4-bromomethylbenzenesulfonyl chloride with one equivalent amount of an amine and then one equivalent amount of bis(t-butoxycarbonylaminoethyl)amine. An intermediate is then obtained after treating the above reaction mixture with hydrochloric acid. Compounds 51-55 can then be respectively prepared by treating this intermediate with a corresponding aldehyde and followed with sodium borohydride. Compounds 50, 67, 112, and 113 can be prepared using the same procedure as that of compounds 51-55 except that 4-bromomethylbenzenesulfonyl chloride is treated with two equivalent amounts of bis(t-butoxycarbonylaminoethyl)amine.
In another example, one can react 4-cyanobenzaldehyde sequentially with one equivalent amount of 2-methyl-2-aminoethanol, sodium borohydride, and 2-(2-bromo-ethoxy)-tetrahydro-pyran to obtain a substituted aminomethylbenzonitrile. Upon removal the tetrahydropyranyl protection group, the benzonitrile can be sequentially mesylated and treated with 2-aminomethylpyridine. After the resultant secondary amine is protected, the benzonitrile can then be treated with diisobutylaluminum, one equivalent amount of a corresponding amine, sodium borohydride, triflic acid, and hydrochloric acid to obtain compounds 114, 118, and 119. A polyamine compound thus synthesized can be further purified by a method such as column chromatography, high-pressure liquid chromatography, or recrystallization.
Other polyamine compounds can be prepared using other suitable starting materials through the above synthetic routes and others known in the art. The methods described above may also additionally include steps, either before or after the steps described specifically herein, to add or remove suitable protecting groups in order to ultimately allow synthesis of the polyamine compounds. In addition, various synthetic steps may be performed in an alternate sequence or order to give the desired compounds. Synthetic chemistry transformations and protecting group methodologies (protection and deprotection) useful in synthesizing applicable polyamine compounds are known in the art and include, for example, those described in R. Larock, Comprehensive Organic Transformations, VCH Publishers (1989); T. W. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, 2nd Ed., John Wiley and Sons (1991); L. Fieser and M. Fieser, Fieser and Fieser's Reagents for Organic Synthesis, John Wiley and Sons (1994); and L. Paquette, ed., Encyclopedia of Reagents for Organic Synthesis, John Wiley and Sons (1995) and subsequent editions thereof.
The polyamine compounds mentioned herein may contain a non-aromatic double bond and one or more asymmetric centers. Thus, they can occur as racemates and racemic mixtures, single enantiomers, individual diastereomers, diastereomeric mixtures, and cis- or trans-isomeric forms. All such isomeric forms are contemplated.
Also within the scope of this invention is a pharmaceutical composition contains an effective amount of at least one polyamine compound described above and a pharmaceutical acceptable carrier. Further, this invention covers a method of administering an effective amount of one or more of the polyamine compounds to a patient having a disease described in the summary section above. This invention also covers a method of administering an effective amount of one or more of the polyamine compounds for enhancing migration of bone marrow-derived cells to blood. “An effective amount” refers to the amount of an active polyamine compound that is required to confer a therapeutic effect on the treated subject. Effective doses will vary, as recognized by those skilled in the art, depending on the types of diseases treated, route of administration, excipient usage, and the possibility of co-usage with other therapeutic treatment.
To practice the method of the present invention, a composition having one or more polyamine compounds can be administered parenterally, enterally (e.g., orally, nasally, and rectally), topically, or buccally. The term “parenteral” as used herein refers to subcutaneous, intracutaneous, intravenous, intramuscular, intraarticular, intraarterial, intrasynovial, intrasternal, intrathecal, intralesional, or intracranial injection, as well as any suitable infusion technique. The compounds also can be administered intravitreally.
A sterile injectable composition can be a solution or suspension in a non-toxic parenterally acceptable diluent or solvent, such as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that can be employed are mannitol, water, Ringer's solution, and isotonic sodium chloride solution. In addition, fixed oils are conventionally employed as a solvent or suspending medium (e.g., synthetic mono- or diglycerides). Fatty acid, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions. These oil solutions or suspensions can also contain a long chain alcohol diluent or dispersant, carboxymethyl cellulose, or similar dispersing agents. Other commonly used surfactants such as Tweens or Spans or other similar emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms can also be used for the purpose of formulation.
A composition for oral administration can be any orally acceptable dosage form including capsules, tablets, emulsions and aqueous suspensions, dispersions, and solutions. In the case of tablets, commonly used carriers include lactose and corn starch. Lubricating agents, such as magnesium stearate, are also typically added. For oral administration in a capsule form, useful diluents include lactose and dried corn starch. When aqueous suspensions or emulsions are administered orally, the active ingredient can be suspended or dissolved in an oily phase combined with emulsifying or suspending agents. If desired, certain sweetening, flavoring, or coloring agents can be added.
A nasal aerosol or inhalation composition can be prepared according to techniques well known in the art of pharmaceutical formulation. For example, such a composition can be prepared as a solution in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other solubilizing or dispersing agents known in the art.
A composition having one or more active polyamine compounds can also be administered in the form of suppositories for rectal administration.
The carrier in the pharmaceutical composition must be “acceptable” in the sense that it is compatible with the active ingredient of the composition (and preferably, capable of stabilizing the active ingredient) and not deleterious to the subject to be treated. One or more solubilizing agents can be utilized as pharmaceutical excipients for delivery of an active polyamine compound. Examples of other carriers include colloidal silicon oxide, magnesium stearate, cellulose, sodium lauryl sulfate, and D&C Yellow # 10.
The polyamine compounds described above can be preliminarily screened for their efficacy in treating above-described diseases by an in vitro assay (See Examples 144-146 below) and then confirmed by animal experiments and clinic trials. Other methods will also be apparent to those of ordinary skill in the art.
Applicants have found that the polyamine compounds, acting as the antagonists of chemokine CXCR4, compete against its ligand SDF-1 for binding to the receptor and thus block CXCR4/SDF-1 signaling, which is important in the mobilization/homing of stem and progenitor cells. Without being bound by theory, the polyamine compounds may act through the following mechanisms in treating and repairing tissue damage.
By blocking CXCR4/SDF-1 signaling, the polyamine compounds promote the mobilization of stem and progenitor cells from bone morrow, a reservoir of stem/progenitor cells, to the peripheral blood. More specifically, as SDF-1 is highly expressed in bone marrow, stem and progenitor cells, expressing CXCR4, are trapped in bone morrow via CXCR4-SDF-1 interaction. By blocking this interaction, the polyamine compounds release stem and progenitor cells from bone marrow to the peripheral blood. While circulating in the blood, stem and progenitor cells home to tissues and organs where damage has occurred and repair the damage by differentiating into the type of cells, the loss of which has caused the damage.
In the condition of retinopathy, SDF-1 is highly expressed in vitreous. Binding to CXCR4 expressed in stem and progenitor cells, SDF-1 facilitates these cells to migrate to the retina, resulting in neovascularization, which plays an essential role in retinopathy development and progression. Also by blocking CXCR4/SDF-1 signaling, the polyamine compounds prevent stem and progenitor cells homing to the retina, thus effectively treating retinopathy. The compounds can be applied topically to an eye of a retinopathy patient. Unlike systemic applications, topical application does not mobilize stem/progenitor cells out of bone marrow and therefore does not help the homing of these cells into retina.
The specific examples below are to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever. Without further elaboration, it is believed that one skilled in the art can, based on the description herein, utilize the present invention to its fullest extent. All publications cited herein are hereby incorporated by reference in their entirety.
Tris(2-aminoethyl)amine (0.01 mol) and 4-fluoro-benzaldehyde (0.03 mol) were dissolved in MeOH (50 mL). After stirring at 25° C. for 15 h, NaBH4 (1.90 g, 0.05 mol) was added to the above solution at 0° C. The reaction mixture was stirred for 2 h at 25° C. It was then diluted with CH2Cl2 (100 mL) and ammonium chloride aqueous solution (10%, 70 mL).
The organic layer was separated, washed with water (100 mL), dried over MgSO4 (s), and concentrated under reduced pressure to yield an oil product. The crude product was purified using alumina column chromatography (EtOAc/MeOH=8:2) to afford compound 1.
LC/MS (M++1): 471.
Compound 2 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 621.
Compound 3 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 525.
Compound 4 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 417.
Compound 5 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 574.
Compound 6 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 471.
Compound 7 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 477.
Compound 8 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 567.
Compound 9 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 624.
Compound 10 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 534.
Compound 11 was prepared in a manner similar to that described in Example 1.
LC/MS (M++1): 426.
1,4-Dibromoxylene (0.012 mol) was treated with bis(tert-butoxyaminoethyl)amine (0.024 mol) in the presence of K2CO3 (0.5 mol) in CH3CN (60 mL) at 60° C. After stirring for 12 h, the solution was allowed to cool down to room temperature, filtered, and concentrated. The concentrate was then treated with HCl/ether and neutralized with K2CO3 to afford 1,4-di[bis(2-aminoethyl)amino-methyl]benzene quantitatively.
1,4-Di[bis(2-aminoethyl)amino-methyl]benzene (0.01 mol) thus obtained and 4-fluoro-benzaldehyde (0.04 mol) were dissolved in MeOH (50 mL). After stirring at 25° C. for 15 h, NaBH4 (2.28 g, 0.06 mol) was added at 0° C. to the above solution. The reaction mixture was stirred for another 2 h at 25° C. It was then diluted with CH2Cl2 (100 mL) and ammonium chloride aqueous solution (10%, 70 mL). The organic layer was separated, washed with water (100 mL), dried over MgSO4 (s), and concentrated under reduced pressure to yield an oil product The crude product was purified using alumina column chromatography (EtOAc/MeOH=7:3) to afford compound 12.
LC/MS (M++1): 741.
Compound 13 was prepared in a manner similar to that described in Example 12.
LC/MS (M++1): 741.
Compound 14 was prepared in a manner similar to that described in Example 12.
LC/MS (M++1): 673.
1,4-Di[bis(2-aminoethyl)amino-methyl]benzene (0.01 mol) and pyridine-2-carbaldehyde (0.02 mol) were dissolved in MeOH (40 mL). After stirring at 25° C. for 15 h, NaBH4 (1.14 g, 0.03 mol) was added to this solution at 0° C. The reaction mixture was stirred for another 2 h at 25° C. It was then diluted with CH2Cl2 (100 mL) and an ammonium chloride aqueous solution (10%, 70 mL). The organic layer was separated, washed with water (100 mL), dried over MgSO4 (s), and concentrated under reduced pressure to yield an oil product. The crude product was purified using alumina column chromatography (EtOAc/MeOH=6:4) to afford compound 15.
LC/MS (M++1): 491.
Bis(2-tert-butoxycarbonylaminoethyl)amine (0.01 mol), 4-cyanobenzylbromide (0.01 mol), and K2CO3 (0.05 mol) in CH3CN (70 mL) were heated at 60° C. for 10 h. The resultant bis(2-tert-butoxycarbonylaminoethyl)amino-4-methylphenylcyanide was deprotected with HCl/ether and condensed with the 4-fluoro-benzaldehyde (0.02 mol) in MeOH. After sequential treatments with NaBH4, di-tert-butyl dicarbonate, and diisobutylaluminum, bis(2-substituted-aminoethyl)amino-4-methylbenzaldehyde was obtained and further condensed with 4-fluoro-benzylamine to give a Schiff base. The Schiff base was then reduced by NaBH4 and deprotected by reacting with HCl. A crude product was obtained and purified with alumina column chromatography (EtOAc/MeOH=7:3) to afford compound 16.
LC/MS (M++1): 747.
Compound 17 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 513.
Compound 18 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 517.
Compound 19 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 532.
Compound 20 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 569.
Compound 21 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 555.
Compound 21 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 527.
Compound 23 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 565.
Compound 24 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 496.
Compound 25 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 488.
Compound 26 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 566.
Compound 27 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 530.
Compound 28 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 563.
Compound 29 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 543.
Compound 30 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 543.
Compound 31 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 513.
Compound 32 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 548.
Compound 33 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 566.
Compound 34 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 578.
Compound 35 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 578.
Compound 36 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 562.
Compound 37 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 513.
Compound 38 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 520.
Compound 39 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 613.
Compound 40 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 527.
Compound 41 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 558.
Compound 42 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 562.
Compound 43 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 609.
Compound 44 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 633.
Compound 45 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 609.
Compound 46 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 575.
Compound 47 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 569.
Compound 48 was prepared in a manner similar to that described in Example 16.
LC/MS (M++1): 667.
K2CO3 (0.05 mol) was added to a solution of tri-Boc-protected cyclam (0.01 mole) and 1,4 dibromomethylbenzene (0.01 mol) in CH3CN at 60° C. After stirring the reaction mixture for 12 h, tri-Boc-protected bromomethylbenzylcyclam was obtained (0.007 mol). It was then reacted with bis(2-pyridyliminoethyl)amine (0.01 mole) in CH3CN (100 mL) in the presence of K2CO3 (0.05 mol) at 60° C. After stirring for 12 h, the reaction mixture was filtered and concentrated. MeOH (50 mL) was added to this mixture, followed by the addition of NaBH4 (0.03 mol) at 25° C. The mixture was stirred for another 2 h. The solution was partitioned between EtOAc and water. The organic layer was then separated, dried over MgSO4 (s), filtered, and concentrated to give a residue. The residue was treated with HCl/ether and purified by alumina column chromatography (EtOAc/MeOH=1:2) to afford compound 49.
LC/MS (M++1): 588.
4-Bromomethylbenzenesulfonyl chloride (0.01 mol) was treated with bis(2-tert-butoxycarbonylaminoethyl)amine (0.02 mol) in CH3CN (100 mL) in the presence of K2CO3 (0.1 mol) at 60° C. After stirring for 12 h, the solution was filtered and the filtrate was concentrated to give a residue. The residue was then treated with HCl/ether and neutralized to give a polyamine. This polyamine was treated with the pyridine-2-carbaldehyde to give a Schiff base. The Schiff base was then reduced by NaBH4 in MeOH. The crude product thus obtained was purified by alumina column chromatography (EtOAc/MeOH=1:1) to afford compound 50.
LC/MS (M++1): 723.
4-Bromomethylbenzenesulfonyl chloride (0.01 mol) and 2-aminomethylpyridine (0.01 mol) were dissolved in ether (100 mL), which contains Et3N (0.02 mol). After stirring for 5 h at 25° C., the solution was washed with water. The resultant bromosulfamide (0.01 mol) was treated with bis(2-tert-butoxycarbonylaminoethyl)amine (0.01 mol) in CH3CN (100 mL) in the presence of K2CO3 (0.05 mol) at 60° C. After stirring for 12 h, the reaction mixture was filtered and the filtrate was concentrated to give a residue. This residue was treated with HCl/ether and neutralized to give a polyamine. The polyamine was then treated with 3,4-dichlorobenzaldehyde to give a Schiff base. The Schiff base was reduced by NaBH4 in MeOH. The crude product thus obtained was purified by alumina column chromatography (EtOAc/MeOH=6:4) to afford compound 51.
LC/MS (M++1): 680.
Compound 52 was prepared in a manner similar to that described in Example 51.
LC/MS (M++1): 616.
Compound 53 was prepared in a manner similar to that described in Example 51.
LC/MS (M++1): 580.
Compound 54 was prepared in a manner similar to that described in Example 51.
LC/MS (M++1): 612.
Compound 55 was prepared in a manner similar to that described in Example 51.
LC/MS (M++1): 612.
1,4-Dibromoxylene (13.64 mmol) was treated with bis(tert-butoxyaminoethyl)amine (2.74 mmol) in the presence of Et3N (2.74 mmol) in CH2Cl2 (50 mL) at 0° C. After stirring for 16 h, the solution was filtered, concentrated, and purified to afford a mono-substituted bromide. This mono-substituted bromide (0.68 mmol) was reacted with 6-methoxy-1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole (0.68 mmol) in CH3CN (10 mL) in the presence of K2CO3 (3.39 mmol) at 60° C. After stirring for 12 h, the solution was filtered and the filtrate was concentrated and purified by chromatography to give a Boc-protected residue (0.57 mmol; 84% yield). This residue (0.26 mmol) was treated with HCl/ether and neutralized to give a polyamine. This polyamine was then treated with the pyridine-2-carbaldehyde to give a Schiff base. The Schiff base was reduced by NaBH4 in MeOH. The crude product thus obtained was purified by alumina column chromatography (EtOAc/MeOH=7:3) to afford compound 56.
LC/MS (M++1): 590.
Compound 57 was prepared in a manner similar to that described in Example 56.
LC/MS (M++1): 560.
Bis(2-tert-butoxycarbonylaminoethyl)amine (0.01 mol), 4-cyanobenzylbromide (0.01 mol), and K2CO3 (0.05 mol) were added in CH3CN (70 mL) and the mixture were heated at 60° C. while stirring for 10 h. The resultant bis(2-tert-butoxycarbonylaminoethyl)amino-4-methylphenylcyanide was deprotected by treating with HCl/ether and then condensed with pyridine-2-benzaldehyde (0.02 mol) in MeOH. After sequential treatments with NaBH4, di-tert-butyl dicarbonate, and DIBAL, the resultant bis(2-substituted-aminoethyl)amino-4-methylbenzaldehyde was condensed with isonicotinic acid hydrazide to give a Schiff base. The Schiff base was then treated with HCl/ether. The crude product thus obtained was purified by alumina column chromatography (EtOAc/MeOH=7:3) to afford compound 58.
LC/MS (M++1): 523.
Compound 59 was prepared in a manner similar to that described in Example 58.
LC/MS (M++1): 528.
Compound 60 was prepared in a manner similar to that described in Example 16 (Yield: 80%).
LC-MS (C35H43N7O.7HCl) (M++1-7HCl): 578.
Compound 61 was prepared in a manner similar to that described in Example 16 (Yield: 80%).
LC-MS (C35H43N7O.7HCl) (M++1-7HCl): 606.
Compound 62 was prepared in a manner similar to that described in Example 16 (Yield: 81%).
LC-MS (C33H42N6O.6HCl) (M++1-6HCl): 539.
Compound 63 was prepared in a manner similar to that described in Example 16 (Yield: 85%).
LC-MS (C33H42N6O.6HCl) (M++1-6HCl): 539.
Compound 64 was prepared in a manner similar to that described in Example 16 (Yield: 78%).
LC-MS (C35H43N7O.7HCl) (M++1-7HCl): 578.
Compound 65 was prepared by a similar manner to that described in Example 56.
LC/MS (M++1): 618.
Compound 66 was prepared by a similar manner to that described in Example 56.
LC/MS (M++1): 666.
Bis(2-tert-butoxycarbonylaminoethyl)amine (3.03 g, 0.01 mol) was added to a solution of 1-bromomethylbenzene-4-sulfonyl chloride (2.68 g, 0.01 mol), CH2Cl2 (160 mL), and Et3N (1.01 g, 0.01 mol). The reaction mixture was stirred at 0° C. for 2.5 hours. Then, the solvent was evaporated and the residue was dissolved in a mixture of CH3CN (180 mL), K2CO3 (4.14 g, 0.03 mol), and 1,2,3,4-tetrahydro-9H-pyrido-6-methoxy[3,4-b]indole (1.72 g, 0.01 mol). The reaction mixture was stirred at 60° C. for another 10 hours. The mixture was then filtered, concentrated, and treated with a mixture of CH2Cl2 (35 mL) and HCl/ether (1.0 M, 80 mL) for 12 hours. The reaction mixture was subsequently concentrated, stirred with anhydrous K2CO3 (10.0 g, 30 min) in CH2Cl2 (150 mL). The mixture thus obtained was filtered and concentrated to afford intermediate 1,1-[bis[(2-aminoethyl)]aminosulfonyl]-4-[(1,2,3,4-tetrahydro-9H-pyrido-6-methoxy[3,4-b]indol-2-methyl)benzene (2.56 g, 0.006 mol) in 60% yield. This intermediate was then treated with pyridine-2-carboxaldehyde (1.50 g, 0.014 mol) in MeOH (40 mL) for 14 hours and then with NaBH4 (1.60 g, 0.042 mol) for 4 hours to give a crude intermediate 2. Intermediate 2 was purified using alumina column chromatography (EtOAc/MeOH=7:3) (3.19 g, 0.005 mol, 83% yield). Intermediate 2 was subsequently treated with HCl/ether (125 mL) in CH2Cl2 (50 mL) to afford compound 67.
LC-MS (C35H41N7O3S.6HCl) (M++1-6HCl): 640.
Compound 68 was prepared by selective alkylation of compound 56 (Yield: 40%).
LC-MS (C38H47N7O2.7HCl) (M++1-7HCl): 634.
Compound 69 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (C33H40N6O2.6HCl) (M++1-6HCl): 553.
Compound 70 was prepared in a manner similar to that described in Example 16 (Yield: 75%).
LC-MS (C35H41N7O3.7HCl) (M++1-7HCl): 608.
Compound 71 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (C37H47N7.7HCl) (M++1-7HCl): 590.
Compound 72 was prepared in a manner similar to that described in Example 16 (Yield: 80%).
LC-MS (C34H43ClN6.6HCl) (M++1-6HCl): 571.
Compound 73 was prepared in a manner similar to that described in Example 16 (Yield: 84%).
LC-MS (C34H42N8.7HCl) (M++1-6HCl): 563.
Compound 74 was prepared in a manner similar to that described in Example 16 (Yield: 71%).
LC-MS (C34H44N6O2.6HCl) (M++1-6HCl): 569.
Compound 75 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (C33H41ClN6O.6HCl) (M++1-6HCl): 573.
Compound 76 was prepared in a manner similar to that described in Example 16 (Yield: 68%).
LC-MS (C33H42N6O2.6HCl) (M++1-6HCl): 555.
Compound 77 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (C33H42N6O2.6HCl) (M++1-6HCl): 555.
Compound 78 was prepared in a manner similar to that described in Example 16 (Yield: 77%).
LC-MS (C33H39BrN6O2.6HCl) (M++1-6HCl): 631.
Compound 79 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (C45H51N7O.7HCl) (M++1-7HCl): 706.
Compound 80 was prepared in a manner similar to that described in Example 56.
LC-MS (C35H40ClN7.7HCl) (M++1-7HCl): 594.
Compound 81 was prepared in a manner similar to that described in Example 56.
LC-MS (C37H47N7.7HCl) (M++1-7HCl): 590.
Compound 82 was prepared in a manner similar to that described in Example 56.
LC-MS (C34H43ClN6.6HCl) (M++1-6HCl): 571.
Compound 83 was prepared in a manner similar to that described in Example 56 (Yield: 80%).
LC-MS (C29H40N6O.6HCl) (M++1-6HCl): 489.
Compound 84 was prepared in a manner similar to that described in Example 56 (Yield: 80%).
LC-MS (C29H40N6O.6HCl) (M++1-6HCl): 489.
Compound 85 was prepared in a manner similar to that described in Example 16.
LC-MS (C39H46N6O.6HCl) (M++1-6HCl): 615.
Compound 86 was prepared in a manner similar to that described in Example 56.
LC-MS (C35H40FN7.7HCl) (M++1-7HCl): 578.
Compound 87 was prepared in a manner similar to that described in Example 16 (Yield: 76%).
LC-MS (C33H39ClN6O30.6HCl) (M++1-6HCl): 603.
Compound 88 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (C31H38ClN6O2S.6HCl) (M++1-6HCl): 559.
Compound 89 was prepared in a manner similar to that described in Example 16 (Yield: 78%).
LC-MS (C34H41ClN6.6HCl) (M++1-6HCl): 569.
Compound 90 was prepared in a manner similar to that described in Example 16 (Yield: 80%).
LC-MS (C33H41ClN6O.6HCl) (M++1-6HCl): 573.
Compound 91 was prepared in a manner similar to that described in Example 56 (Yield: 65%).
LC-MS (C37H45N7O.7HCl) (M++1-7HCl): 604.
Compound 92 was prepared in a manner similar to that described in Example 16 (Yield: 85%).
LC-MS (C31H38N6O2S.6HCl) (M++1-6HCl): 559.
Compound 93 was prepared in a manner similar to that described in Example 56.
LC-MS (C34H42N6O.6HCl) (M++1-6HCl): 551.
Compound 94 was prepared in a manner similar to that described in Example 56.
LC-MS (C35H44N6O.6HCl) (M++1-6HCl): 565.
Compound 95 was prepared in a manner similar to that described in Example 56.
LC-MS (C35H43ClN6O.6HCl) (M++1-6HCl): 599.
Compound 96 was prepared in a manner similar to that described in Example 16.
LC-MS (C33H40N6.6HCl) (M++1-6HCl): 521.
Compound 97 was prepared in a manner similar to that described in Example 16.
LC-MS (C31H36Cl2N6.6HCl) (M++1-6HCl): 563.
Compound 98 was prepared in a manner similar to that described in Example 16.
LC-MS (C31H37ClN6.6HCl) (M++1-6HCl): 529.
Compound 99 was prepared in a manner similar to that described in Example 16.
LC-MS (C33H42N6O2.6HCl) (M++1-6HCl): 555.
Compound 100 was prepared in a manner similar to that described in Example 16.
LC-MS (C37H45N7O2.6HCl) (M++1-6HCl): 620.
Compound 101 was prepared in a manner similar to that described in Example 16.
LC-MS (C37H47N7O.7HCl) (M++1-7HCl): 606.
Compound 102 was prepared in a manner similar to that described in Example 16 (Yield: 80%).
LC-MS (C33H41ClN6O.6HCl) (M++1-6HCl): 573.
Compound 103 was prepared in a manner similar to that described in Example 16.
LC-MS (C36H45N7.7HCl) (M++1-7HCl): 576.
Compound 104 was prepared in a manner similar to that described in Example 16.
LC-MS (C33H41ClN6.6HCl) (M++1-6HCl): 557.
Compound 105 was prepared in a manner similar to that described in Example 16.
LC-MS (C33H40N8.7HCl) (M++1-7HCl): 549.
Compound 106 was prepared in a manner similar to that described in Example 56 (Yield: 65%).
LC-MS (C37H45N7O2.7HCl) (M++1-7HCl): 620.
Compound 107 was prepared in a manner similar to that described in Example 56 (Yield: 82%).
LC-MS (C30H42N6O.6HCl) (M++1-6HCl): 503.
Compound 108 was prepared in a manner similar to that described in Example 56.
LC-MS (C37H45N7O2. 7HCl) (M++1-7HCl): 620.
Compound 109 was prepared in a manner similar to that described in Example 56.
LC-MS (C35H42N6.6HCl) (M++1-6HCl): 547.
A mixture of bis(2-tert-butoxycarbonylaminoethyl)amine (0.01 mol), 4-cyanobenzyl-bromide (0.01 mol), K2CO3 (0.05 mol), and CH3CN (70 mL) was heated at 60° C. for 10 hours.
The resultant bis(2-tert-butoxycarbonylaminoethyl)amino-4-methylphenylcyanide was deprotected using HCl/ether, condensed with 2-acetyl pyridine (0.01 mol) in MeOH, and then reduced by NaBH4. After sequential treatments with pyridine-2-carboxaldehyde, NaBH4, and HCl, compound 110 was obtained in 75% overall yield.
LC-MS (C25H30N6.5HCl) (M++1-5HCl): 415.
A mixture of bis(2-tert-butoxycarbonylaminoethyl)amine (0.01 mol), 4-cyanobenzyl-bromide (0.01 mol), K2CO3 (0.05 mol), and CH3CN (70 mL) was heated at 60° C. for 10 hours. The resultant bis(2-tert-butoxycarbonylaminoethyl)amino-4-methylphenylcyanide was deprotected by HCl/ether, condensed with 2-acetyl pyridine (0.01 mol) in MeOH, and then reduced by NaBH4. After sequential treatments with pyridine-2-carboxaldehyde, NaBH4, di-tert-butyl dicarbonate, and diisobutylaluminum, the aldehyde thus obtained was condensed with 2-aminomethylbenzimidazole to give a Schiff base. The Schiff base was then reduced by NaBH4 and deprotected by HCl to afford compound III in 45% overall yield.
LC-MS (C33H40N8.7HCl) (M++1-7HCl): 549.
Compound 112 was prepared in a manner similar to that described in Example 67.
LC-MS (C36H46N6O2.6HCl) (M++1-6HCl): 595.
Compound 113 was prepared in a manner similar to that described in Example 56.
LC-MS (C36H46N6O2.6HCl) (M++1-6HCl): 595.
4-Cyanobenzaldehyde (0.01 mol) was treated with 2-methyl-2-aminoethanol (0.01 mol) in MeOH (20 mL) at 60° C. for 12 hours. NaBH4 (1.90 g, 0.05 mol) was then added to the above solution at 0° C. The reaction mixture was stirred for another 2 hours at 25° C. It was then diluted with CH2Cl2 (100 mL) and with an ammonium chloride aqueous solution (10%, 70 mL). The organic layer was separated, washed with water (100 mL), dried over MgSO4 (s), and concentrated under reduced pressure to yield an oil intermediate. The oil intermediate was then condensed with 2-(2-bromo-ethoxy)-tetrahydro-pyran (0.01 mol) in the presence of K2CO3 (0.05 mol) in refluxing CH3CN (30 mL). After deprotection, the resultant hydroxyl group was mesylated and was allowed to react with 2-aminomethylpyridine. The resultant secondary amine was then protected with the Boc group. Subsequently, the cyanide group was treated with diisobutylaluminum and the aldehyde thus obtained was sequentially treated with 6-methyl-3-aminoethylindol, NaBH4, triflic acid, and HCl to afford compound 114 in 60% overall yield.
LC-MS (C36H45N7.6HCl) (M++1-6HCl): 576.
Compound 115 was prepared in a manner similar to that described in Example 16 (Yield: 77%).
LC-MS (M++1): 559.
Compound 116 was prepared in a manner similar to that described in Example 111 (Yield: 70%).
LC-MS (M++1): 527.
Compound 117 was prepared in a manner similar to that described in Example 111 (Yield: 70%).
LC-MS (M++1): 576.
Compound 118 was prepared in a manner similar to that described in Example 114 (Yield: 68%).
LC-MS (M++1): 549.
Compound 119 was prepared in a manner similar to that described in Example 114 (Yield: 69%).
LC-MS (M++1): 557.
Compound 120 was prepared in a manner similar to that described in Example 111 followed by reaction with acetaldehyde and NaBH4 (Yield: 75%).
LC-MS (M++1): 590.
Compound 121 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (M++1): 573.
Compound 122 was prepared in a manner similar to that described in Example 16 (Yield: 67%).
LC-MS (M++1): 645.
Compound 123 was prepared in a manner similar to that described in Example 111 (Yield: 73%).
LC-MS (M++1): 606.
Compound 124 was prepared in a manner similar to that described in Example 111 (Yield: 60%).
LC-MS (M++1): 579.
Compound 125 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (M++1): 535.
Compound 126 was prepared in a manner similar to that described in Example 16 (Yield: 85%).
LC-MS (M++1): 557.
Compound 127 was prepared in a manner similar to that described in Example 16 (Yield: 80%).
LC-MS (M++1): 543.
Compound 128 was prepared in a manner similar to that described in Example 16 (Yield: 68%).
LC-MS (C33H42N6O2) (M++1): 555.
Compound 129 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (C33H42N6O2) (M++1): 555.
Compound 130 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (C33H41ClN6O) (M++1): 573.
Compound 131 was prepared in a manner similar to that described in Example 16.
LC-MS (C33H42N6O2) (M++1): 555.
Compound 132 was prepared in a manner similar to that described in Example 16 (Yield: 70%).
LC-MS (C32H40N6O2) (M++1): 541.
Compound 133 was prepared in a manner similar to that described in Example 16 (Yield: 73%).
LC-MS (C32H39ClN6O) (M++1): 559.
Compound 134 was prepared in a manner similar to that described in Example 16 (Yield: 67%).
LC-MS (C39H46N6O2) (M++1): 631.
Compound 135 was prepared in a manner similar to that described in Example 16 (Yield: 67%).
LC-MS (C38H44N6O2) (M++1): 617.
Compound 136 was prepared in a manner similar to that described in Example 16 (Yield: 73%).
LC-MS (C33H40N8O) (M++1): 566.
Compound 137 was prepared in a manner similar to that described in Example 16 (Yield: 78%).
LC-MS (C36H45N7O) (M++1): 592.
Compound 138 was prepared in a manner similar to that described in Example 16 (Yield: 78%).
LC-MS (C35H43N7O) (M++1): 578.
Compound 139 was prepared in a manner similar to that described in Example 16 (Yield: 80%).
LC-MS (C35H43N7O) (M++1): 578.
Compound 140 was prepared in a manner similar to that described in Example 16 (Yield: 80%).
LC-MS (C35H43N7) (M++1): 562.
Compound 141 was prepared in a manner similar to that described in Example 16.
LC-MS (C33H40N6) (M++1): 521.
1,4-Dibromoxylene (13.64 mmol) was treated with bis(tert-butoxyaminoethyl)amine (2.74 mmol) in the presence of Et3N (2.74 mmol) in CH2Cl2 (50 mL) at 0° C. After stirring for 16 hours, the solution was filtered, concentrated, and purified to afford a mono-substituted bromide. This mono-substituted bromide (0.68 mmol) was reacted with 6-methoxy-1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole (0.68 mmol) in CH3CN (10 mL) in the presence of K2CO3 (3.39 mmol) at 60° C. After stirring for 12 hours, the solution was filtered and the filtrate was concentrated and purified by chromatography to give a Boc-protected residue (0.57 mmol; 84% yield). This residue (0.26 mmol) was treated with HCl/ether and neutralized to give a polyamine. This polyamine was then treated with the pyridine-2-carbaldehyde to give a Schiff base, which was then reduced by NaBH4 in MeOH. The crude product thus obtained was purified by alumina column chromatography (EtOAc/MeOH=7:3) to afford compound 142.
LC-MS (C36H43N7O) (M++1): 590.
Compound 143 was prepared in a manner similar to that described in Example 142 (Yield: 80%).
LC-MS (C29H40N6O) (M++1): 489.
Compounds 1-126 and 137-143 were tested for their efficacy in binding to CXCR4 receptor using a DELFIA GTP-binding kit (Wallac Oy, Turku, Finland). The DELFIA GTP-binding assay is a time-resolved fluorometric assay based on GDP-GTP exchange on G-protein subunits followed by activation of a G protein-coupled receptor by its agonists. Eu-GTP, obtained from Wallac Oy, was used in this assay to allow monitoring of agonist-dependent activation of G-protein. Stimulation of CXCR4 receptor by SDF-1 leads to the replacement of GDP by GTP on the α-subunit of G-protein. This GTP-Gα complex represents the activated form of G-protein. Eu-GTP, a non-hydrolysable analog of GTP, can be used to quantify the amount of activated G-protein. (Peltonen et al., Eur. J. Pharmacol. (1998) 355:275.)
Plasma membrane of CXCR4-expressing HEK293 cells was suspended in an assay buffer (50 mM NaCl, 100 μg/mL saponin, 3 mM MgCl2, 3 μM GDP, 5% BSA, 50 mM HEPES, pH 7.4). An aliquot (4 μg protein) was added to each well of an AcroPlate (Pall Life Sciences, Ann Arbor, Mich.). After the addition of the test compounds (10 μM in 0.1% DMSO) and stromal-derived factor-1 (4 nM in the assay buffer), the assay plate was incubated in the dark at room temperature with slow shaking for 10 minutes. Eu-GTP was added to each well and the plate was incubated again for 60 minutes. The assay was terminated by washing the plate twice with a wash solution provided in the assay kit. Binding of Eu-GTP was determined based on the fluorescence signal from a Victor 2 multi-label reader.
Unexpectedly, all of the tested compounds showed IC50 values lower than 10 μM. Specifically, 104 of the test compounds showed IC50 values lower than 1 μM. Among them, 63 showed IC50 values between 0.004 μM and 0.1 μM.
Competition binding assays with between test compounds and human stromal-derived factor-1 (SDF-1) were carried out using glass fiber filter plates (Millipore, Billerica, Mass.). Glass fiber filter plates were pre-coated with 90 μl of 0.2% polyethyleneimine for 30 minutes and rinsed with 100 μl of distilled water for four times to reduce non-specific binding. Membranes of human CXCR4-transfected HEK293 cells (5-10 μg protein/well) in a 70 μl assay buffer (50 mM HEPES, pH 7.4, 0.5% bovine serum albumin, 90 mM NaCl, 5 mM MgCl2, 1 mM CaCl2) were incubated with 20 μl of each test compound and 10 μl of [125I]-SDF-1 (final concentration 150 pM) in U-bottom assay plates (Corning, Corning, N.Y.). After 120 minutes at room temperature, incubation was terminated by transferring 80 μl of reaction mixture to each glass fiber plate well and filtered by vacuum filtration (MultiScreen Vacuum Maniford, Millipore). The plate was washed 4 times with 80 μl/well of wash buffer (20 mM HEPES, pH 7.4 and 90 mM NaCl). The plate was air dried overnight and the radioactivity retained on the filter was determined by adding 35 μl/well of Supermix cocktail and then counted with Trilux MicroBeta (PerkimElmer, Boston, Mass.).
Compounds 94 and 137-143 were tested. Unexpectedly, all of the test compounds showed IC50 values in radioligand binding assay in the range of 11-84 nM.
Animal studies showed that compounds 125 and 136 rapidly mobilized hematopoietic stem cells CD34+ and EPCs CD133+ into peripheral blood. The in vivo efficacy of these two compounds in mobilizing stem cells was tested in male Special Pathogens Free (SPF) BALB/c mice. Different concentrations of a compound solution were prepared and administrated either intravenously of intramuscularly into mice. Mice receiving saline were used as a control. Whole blood was collected at 3 hours after i.v. injection or 6 hours after i.m. injection by cardiac puncture. Total leukocytes were resuspended in PBS and cell numbers were counted using trypan blue exclusion. Cells numbers were adjusted to 6×104 in 0.1 mL PBS for antibody staining. CXCR4+ cells, CD34+ cells, and CD133+ cells were counted using surface staining and flow cytometry analysis. Data represents mean ±SEM of four independent experiments. Levels of cell number in different groups were compared using One-way ANOVA, differences were considered significant if P values were <0.05. The results showed that, within 3 hours after a single i.v. injection, compounds 125 and 136 increased circulation of CD34+ cells and CD133+ cells up to 6 to 8.5-folds. The results are summarized in Table 1 below.
G-CSF is a growth factor for stem cells and EPCs currently used in clinics to improve hematopoietic function in cancer patients after chemotherapy. Compound 125, when used in combination with G-CSF, showed synergistic effect in stem cell and EPC mobilization. Specifically, a combination of compound 125 and G-CSF dramatically increased circulation of CD34+ cells up to 38 folds and circulation of CD133+ cells up to 64-folds. The results are summarized in Table 2 below.
All of the features disclosed in this specification may be combined in any combination. Each feature disclosed in this specification may be replaced by an alternative feature serving the same, equivalent, or similar purpose. Thus, unless expressly stated otherwise, each feature disclosed is only an example of a generic series of equivalent or similar features.
From the above description, one skilled in the art can easily ascertain the essential characteristics of the present invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Thus, other embodiments are also within the scope of the following claims.
For example, the polyamine compounds described above can be used to treat an inflammatory or immune disease through mechanisms other than binding to CXCR4 receptor. Further, other uses of these compounds are also within the scope of this invention.
This application is a continuation-in-part application of U.S. patent application Ser. No. 10/814,058, filed Mar. 30, 2004, now U.S. Pat. No. 7,399,776, which claims priority from U.S. Provisional Patent Application No. 60/459,768, filed Apr. 2, 2003 and U.S. Provisional Patent Application No. 60/539,763, filed Jan. 28, 2004. The present application also claims priority from U.S. Provisional Patent Application No. 60/836,168, filed Aug. 8, 2006. The contents of these related applications are incorporated herein by reference in their entireties.
| Number | Name | Date | Kind |
|---|---|---|---|
| 5252317 | Keana | Oct 1993 | A |
| 5567411 | Keana et al. | Oct 1996 | A |
| 5719193 | Bowlin et al. | Feb 1998 | A |
| 5910513 | Galey | Jun 1999 | A |
| 7399776 | Shia et al. | Jul 2008 | B2 |
| 20050043366 | Shia et al. | Feb 2005 | A1 |
| 20050165063 | Yamazaki et al. | Jul 2005 | A1 |
| Number | Date | Country |
|---|---|---|
| 62129858 | Jun 1987 | JP |
| 64-66117 | Mar 1989 | JP |
| 1-272568 | Oct 1989 | JP |
| 2002-543126 | Dec 2002 | JP |
| 3714948 | Sep 2005 | JP |
| WO 9700245 | Jan 1997 | WO |
| WO 0002870 | Jan 2000 | WO |
| WO 0056729 | Sep 2000 | WO |
| WO 0066112 | Nov 2000 | WO |
| WO 0222600 | Mar 2002 | WO |
| WO 02055112 | Jul 2002 | WO |
| WO 02083143 | Oct 2002 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 20080058382 A1 | Mar 2008 | US |
| Number | Date | Country | |
|---|---|---|---|
| 60459768 | Apr 2003 | US | |
| 60539763 | Jan 2004 | US | |
| 60836168 | Aug 2006 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 10814058 | Mar 2004 | US |
| Child | 11834204 | US |
