Claims
- 1. Microspheres having a diameter from 200 A to 10.mu. consisting essentially of water-insoluable copolymer of glutaraldehyde having a repeating conjugated aldehyde containing unit of the formula: ##STR2## cross linked with 0.1 to 10% by weight of a water-dispersible or water-soluble organic cross-linking agent containing at least two functional groups reactive with said aldehyde group selected from amine, hydroxyl, imine mercaptan or amide and said microspheres containing 0.5 to 40% by weight of metal containing particles having an atomic number above 50 and a diameter below 1000 A.
- 2. Microspheres according to claim 1 in which the cross-linking agent is also a surfactant.
- 3. Microspheres according to claim 2 in which the cross-linking agent is an acrylamide polymer.
- 4. Microspheres according to claim 3 in which the cross-linking agent is a copolymer of 10 to 70% acrylamide and 10 to 70% of an alkyl-acrylamide or an alkyloxyalkyl acrylamide.
- 5. Microspheres according to claim 1 in which the cross-linking agent contains 2 to 20 carbon atoms.
- 6. Microspheres according to claim 5 in which the cross-linking agent is a diamine.
- 7. Microspheres according to claim 1 in which the particles are magnetically attractable.
- 8. Microspheres according to claim 7 in which the particles comprise Fe.sub.3 O.sub.4.
- 9. Microspheres according to claim 1 containing fluorogen groups.
- 10. Microspheres according to claim 9 in which the fluorogen groups are the reaction products for acrolein groups and a compound that forms a fluorogen on reaction with said groups.
- 11. Microspheres according to claim 9 in which the fluorogen is the reaction product of aldehyde groups with an aldehyde reactive fluorochrome.
- 12. Microspheres according to claim 11 in which the fluorochrome is selected from aminofluorescein, 9-amino acridine, propidium bromide, or fluorescein isothiocyanate.
- 13. Microspheres according to claim 1 bound to protein through said aldehyde groups.
- 14. Microspheres according to claim 13 in which the protein is selected from antibodies, antigens, immunoglobulins, lymphocytes or hemoglobin.
- 15. Microspheres according to claim 14 in which the protein is an antibody.
ORIGIN OF THE INVENTION
The invention described herein was made in the course of work under a grant from the Department of Health, Education and Welfare.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
3957741 |
Rembaum et al. |
May 1976 |
|
4070246 |
Kennedy et al. |
Jan 1978 |
|