Patent Application
20050181441
1. Technical Field
The present invention relates to a biochip for identifying ionic polymers such as represented by DNA and proteins, and a method of identifying ionic polymers using the biochip.
2. Background Art
There is an expectation that identifying DNAs and proteins can provide a tool for drug development and medical examinations.
A DNA is a polymer formed by a sequence of four kinds of bases, and two DNAs can specifically bind to one another through their sequences. Because the nucleic acids of both such DNAs are negatively charged, hybridization is carried out in a highly ionic solvent. The conventional biochips take advantage of this fact to detect a sample DNA by immobilizing a probe DNA on a substrate.
However, a DNA-binding protein, for example, cannot be identified by the conventional biochip. This is because a protein as a sample may be specifically adsorbed on a probe DNA on a biochip by its complementary DNA portion, and the protein may also be nonspecifically adsorbed on the probe DNA even though it is not supposed to, in light of the DNA. Because of such specific and nonspecific adsorptions that apparently occur on the probe DNA, it has been virtually impossible to identify sample proteins using the conventional biochip.
Thus, there is a need to develop a chip that can specifically bind to ionic polymers such as the multitude of proteins, and a technique of accurately identifying ionic polymers using the chip.
The inventors arrived at the present invention after discovering that various ionic polymers such as DNA-binding proteins can be identified in a simple and accurate manner by using a polymer chip with a particular structure.
Specifically, the invention in one aspect provides a polymer chip for identifying an ionic polymer which comprises a substrate on which a first ionic polymer is immobilized, wherein a second ionic polymer having a marker is complementarily bound to the first ionic polymer as a probe, the polymer chip identifying an ionic polymer by having a third ionic polymer as a sample specifically bind thereto.
The first to third ionic polymers are preferably selected from proteins, polyamino acids, DNA, RNA and synthetic polymers, for example.
More specifically, the invention provides a DNA chip for identifying a DNA-binding protein, which comprises a substrate on which a first DNA is immobilized, wherein a second DNA having a marker is complementarily bound to the first DNA.
Preferably, the number of bases in the base sequence of the first DNA and second DNA is from four to 13, respectively. When the number of bases is in this range, the complementary binding of the first and second DNAs is strengthened, and further, the specific binding of the DNA chip comprising these DNAs and a sample DNA-binding protein based on their chemical and physical structures is strengthened, thus making the identification of the sample protein easier and more accurate.
The material and shape of the substrate are not particularly limited, and they can be selected from the materials and shapes that are employed in the substrates of biochips. Preferably, the material is selected from glass, silica gel, polystyrene, polypropylene and membrane, and the substrate is either plate- or bead-shaped.
The marker attached to the second ionic polymer is selected from ones used in the field of biochip technologies. Particularly, substances that emit fluorescence are preferable for the optical processes in a readout step.
In another aspect, the invention provides a method of identifying an ionic polymer which comprises the steps of providing a probe by complementarily binding a second ionic polymer having a marker to a first ionic polymer in an ionic solvent, wherein the first ionic polymer is immobilized on a substrate in a polymer chip, drying the probe, having a third ionic polymer as a sample adsorbed on the probe, washing the probe with an ionic solvent to remove the third ionic polymer that is nonspecifically bound (i.e., one that binds to the probe even though it should not), washing the probe with a nonionic solvent to remove the second ionic polymer having a marker except for a part where the third ionic polymer is specifically bound (when it should be), and reading the amount of the marker that remains.
In the case where the probe in which the second ionic polymer having a marker is complementarily bound to the first ionic polymer has the same charge as that of the sample third ionic polymer, preferably a step is provided in which a fourth sample polymer having an opposite ionicity is added to firmly bind the probe and the sample third ionic polymer, and the probe is then washed with a nonionic solvent.
The first to third ionic polymers are preferably selected from proteins, polyamino acids, DNA, RNA and synthetic polymers, for example.
More specifically, the invention identifies a sample protein by providing a probe by complementarily binding a second DNA having a marker to a first DNA in an ionic solvent, wherein the first DNA is immobilized on a substrate in a DNA chip, drying the probe, having a DNA-binding protein as a sample adsorbed on the probe, washing the probe with an ionic solvent to remove the protein that is nonspecifically bound, washing the probe with a nonionic solvent to remove the second DNA having a marker except for a part where the DNA-binding protein is specifically bound, and reading the amount of the remaining marker.
Preferably, the number of bases in the base sequence of the first DNA and second DNA is from four to 13, respectively. When the number of bases in the base sequence is in this range, the complementary binding of the first and second DNAs is strengthened, and further, the specific binding of the DNA chip comprising these DNAs and a sample DNA-binding protein based on their chemical and physical structures is strengthened, thus making the identification of the sample protein easier and more accurate.
The material and shape of the substrate are not particularly limited, and they can be selected from the materials and shapes that are employed in the substrates of biochips. Preferably, the material is selected from glass, silica gel, polystyrene, polypropylene and membrane, and the substrate is either plate- or bead-shaped.
The marker attached to the second ionic polymer is selected from ones used in the field of biochip technologies. Particularly, a substance that emits fluorescence is preferable for the optical processes in a readout step.
The kind of the non-ionic solvent is not particularly limited. Examples include one selected from the group containing pure water, alcohol, acetone, and hexane, or a mixture of two or more substances therein. The ionic solvent is not particularly limited either, and examples include an aqueous solution of sodium chloride and an aqueous solution of potassium chloride.
Biopolymers such as DNA and proteins (polyamino acids and polypeptides) are charged. DNA has a negative charge due to a phosphate group. Proteins have either a positive or negative charge, depending on the kind of the amino acids. Double strands of DNA normally repel each other due to negative ions and they cannot be hybridized in pure water. In a system in which positive ions exist, however, they can be hybridized by hydrogen bonding in the presence of ions when, for example, an Na+ ion forms a chelate with a phosphate group of DNA. Proteins having a positive charge, for example, can bind to DNA having a negative charge via the charged portions.
When a biochip in which these biopolymers are bonded by their charges is washed with a solvent in which the ion intensity is varied, at low ion intensities polymers having the same charge are dissociated and polymers having opposite charges are firmly bound. At high ion intensities, even the polymers having the same charges can be bound while the bond of polymers having different charges becomes weak and they are dissociated.
By thus varying the ion intensity, various polymers can be identified.
The DNA-binding proteins used in the present invention are those proteins that have an affinity for DNA and specifically or nonspecifically bind to a base sequence. Examples of such DNA-binding proteins include: 1) double-stranded DNA-binding proteins that control gene expression by introducing changes in the DNA structure; 2) single-stranded DNA-binding (SSB) proteins that are indispensable for the processes of DNA replication, recombination and repair; 3) proteins involved in the maintenance of higher structures of chromosomes; 4) DNA-dependent ATPase; and 5) topoisomerases that form DNA superhelix structures. Many of the proteins 1) are transcription factors, for which several structural motifs are known, such as a helix-turn-helix that was found in the structure of a lambda phage Cro protein and a cAMP receptor protein; a zinc finger in which a zinc ion is chelated by cysteines and histidines, and; a leucine zipper that is formed when two protein molecules each having a leucine arranged on a side of a-helix are combined as in a zipper. The proteins 2), namely the SSB proteins, are found in organisms from bacteriophages to higher forms of life. The proteins 3) are represented by a histone protein in the chromosomes of eukaryotes, and they form nucleosome structures. In bacterium too, similar HU proteins bind to form a nucleosome-like structure. The proteins 4) include helicases that promote DNA replication (DnaB protein, for example), recombination (RecA, RecBC proteins), and unwinding of double-stranded DNA. Furthermore, in the present invention, not only the above-mentioned proteins that originally have a DNA-binding property but also proteins that have come to possess a DNA-binding property by physical or chemical processing can be used.
Thus, in the invention, this wide variety of DNA-binding proteins can be used. The invention includes not only the case of using a single kind of DNA-binding protein but also the case of using two or more kinds of DNA-binding proteins in combination.
DNA-binding proteins have a positive charge at the DNA-binding domains, and they are characterized by their specific or nonspecific binding to DNA or RNA having a negative charge. In a double-stranded DNA to which these DNA-binding proteins have bound, the bond becomes stronger the lower the ion intensity.
Hereafter, examples of the invention will be described, in which proteins TRF, c-Myb, and Pur that had already been identified were used as samples. TRF is described by Nishikawa, T., Nagadoi, A., Yoshimura, S., Aimoto, S., and Nishimura, Y. in “Solution structure of the DNA-binding domain of human telomeric protein, hTRF1,” Structure, 6, pp. 1057-1065 (1998). C-Myb is described by Ogata, K., Hojo, H., Aimoto, S., Nakai, T., Nakamura, H., Sarai, A., Ishii, S. and Nishimura, Y. in “A Helix-turn-helix-related motif with conserved tryptophans forming a hydrophobic core,” Proc. Nat. Acad. Sci. USA, 89, pp. 6428-6432 (1992). Pur is described by Nagadoi, A., Morikawa, S., Nakamura, H., Enari, M., Kobayashi, K., Yamamoto, H., Sampei, G., Mizobuchi, K., Schumacher, M. A., Brennan, R. G., and Nishimura, Y. in “Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain,” Structure 3, pp. 1217-1224 (1995).
Initially, a biochip was produced as follows.
A TRF probe DNA with a sequence of 5′-GTTAGGGTTAGGG-3′ and a c-Myb probe DNA with a sequence of 5′-CCTAACTGACACC-3′ were biotinylated at their 5′-ends and spotted at 24 locations on an avidin-coated slide glass by SPBIO (from Hitachi Software Engineering Co., Ltd). The spotted probe DNAs were adjusted to a concentration of 1 mM by adding pure water.
Two strands of sample DNA were prepared. A TRF sample DNA had a sequence of 3′-CAATCCCAATCCC-5′ and a c-Myb sample DNA had a sequence of 3′-GGATTGACTGTGG-5′. They were both labeled with the fluorescent pigment Cy5 at their 5′-ends.
After adjusting the concentration of the sample DNAs to 10 μM in a 5×SSC solution, 20 μL of each sample was added onto the prepared biochip and hybridized for 30 min in a humid environment to prevent drying.
After hybridization, the biochip was washed with 1×SSC and dried, and then read by a fluorescence scanner CRBIO2 (from Hitachi Software Engineering Co., Ltd).
Thereafter, a TRF protein sample liquid prepared from TRF protein at 110 μM, KPB (phosphoric acid) at 5 mM, and NaCl at 30 mM was added onto the hybridized biochip and hybridized for 10 min.
After washing the biochip with 1×SSC at 40° C. and then with pure water at 40° C., the biochip was read by CRBIO2 (from Hitachi Software Engineering Co., Ltd). The image of the individual spots that were read is shown in
Similarly, c-Myb protein at 74 μM, KPB at 10 mM, DTT at 1 mM, and NaN3 at 0.1 mM were hybridized onto the biochip 2. The biochip 2 was then washed with 1×SSC and pure water and read by CRBIO2. The image of the individual spots that were read is shown in
Similarly, Pur protein at 527 μM, KPB at 1 mM, KCl at 20 mM, and NaN3 at 0.05 mM were hybridized on the biochip 3. The biochip 3 was washed with 1×SSC and pure water, and then read by CRBIO2 (from Hitachi Software Engineering Co., Ltd). The image of the individual spots that were read is shown in
It will be seen from
In
Ideally, the spots where there is no decrease in fluorescence intensity have the value of 1.0 and values less than 1.0 are assigned as the fluorescence intensity decreases. It will be seen from
Thus, the present invention can effectively and comprehensively identify ionic polymers such as transfer factor proteins. In particular, the invention can identify a number of proteins easily and accurately. The invention can thus provide a tool for drug development and medical examinations.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2002-090129 | Mar 2002 | JP | national |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 10368451 | Feb 2003 | US |
| Child | 11107845 | Apr 2005 | US |
