Claims
- 1. A method of preparing a polymer-protein composite comprisingion-pairing a protein in an aqueous phase with a surfactant in a first organic phase to yield a protein-surfactant ion pair; contacting the protein-surfactant ion pair with a second organic phase containing at least one selected from the group consisting of the polymer or a monomer that can be polymerized to yield the polymer; removing the second organic phase to yield a polymer-protein composite.
- 2. The method according to claim 1, wherein the second organic phase comprises the monomer.
- 3. The method according to claim 2, wherein the monomer is polymerized by suspension polymerization.
- 4. The method according to claim 2, wherein the monomer is polymerized by emulsion polymerization.
- 5. The method according to claim 1, wherein the second organic phase comprises the polymer.
- 6. The method according to claim 5, wherein the protein is modified chemically with one or more reactive functional groups that can form a covalent bond with the polymer.
- 7. The method according to claim 1, wherein the protein is a naturally-occurring protein.
- 8. The method according to claim 1, wherein the polymer-protein composite comprises from 0.05% to 90% by weight of protein, based on the total weight of the composite.
- 9. The method according to claim 6, wherein the reactive functional group comprises a vinyl group.
- 10. The method according to claim 6, wherein the reactive functional group comprises an acrylate group.
- 11. The method according to claim 1, wherein the protein is an enzyme.
- 12. A method of preparing a polymer-protein composite that comprises(a) contacting and mixing a protein-containing aqueous solution with a surfactant in an organic solution to yield an organic phase containing an protein-surfactant ion pair, (b) separating out the organic phase that contains the ion pair from the aqueous phase that no longer contains the ion pair, (c) removing the organic solution from the organic phase of (b), (d) dissolving the ion-pair in a second organic solution, (e) contacting the dissolved ion-pair of (d) with a polymer that is also dissolved in the second organic solution to form a polymer-protein complex and (f) recovering the polymer-protein complex.
- 13. The method according to claim 12, wherein the protein is modified chemically with one or more reactive functional groups that can form a covalent bond with the polymer.
- 14. The method according to claim 12, wherein the protein is a naturally-occurring protein.
- 15. The method according to claim 12, wherein the polymer-protein composite comprises from 0.05% to 90% by weight of protein, based on the total weight of the composite.
- 16. The method according to claim 13, wherein the reactive functional group comprises a vinyl group.
- 17. The method according to claim 13 wherein the reactive functional group comprises an acrylate group.
- 18. The method according to claim 12, wherein the protein is an enzyme.
- 19. A polymer-protein composite comprising a protein incorporated in a polymer,wherein the polymer-protein composite comprises from about 0.05 to 90% by weight of protein, based on the total weight of the composite, wherein the polymer-protein composite is obtainable by: ion-pairing of a protein in an aqueous phase with a surfactant in a first organic phase to yield a protein-surfactant ion pair; contacting the protein-surfactant ion pair with a second organic phase containing at least one selected from the group consisting of the polymer or a monomer that can be polymerized to yield the polymer; removing the second organic phase to yield a polymer-protein composite.
- 20. The polymer-protein composite according to claim 19, wherein the protein is covalently bound to the polymer.
- 21. A polymer-protein composite comprising a protein incorporated in a polymer,wherein the polymer-protein composite comprises from about 0.05% to 90% by weight of protein, based on the total weight of the composite, wherein the polymer-protein composite is obtainable by (a) contacting and mixing a protein-containing aqueous solution with a surfactant in an organic solution to yield an organic phase containing an protein-surfactant ion pair, (b) separating out the organic phase that contains the ion pair from the aqueous phase that no longer contains the ion pair, (c) removing the organic solution from the organic phase of (b), (d) dissolving the ion-pair in a second organic solution, (e) contacting the dissolved ion-pair of (d) with a polymer that also is dissolved in the second organic solution to form a polymer-protein complex and (f) recovering the polymer-protein complex.
- 22. The polymer-protein composite according to claim 21, wherein the protein is covalently bound to the polymer.
- 23. The method according to claim 12, wherein the polymer is formed from a monomer that is polymerized by suspension polymerization.
- 24. The method according to claim 12, wherein the polymer is formed from a monomer that is polymerized by emulsion polymerization.
- 25. The method according to claim 19, wherein the monomer is polymerized by suspension polymerization.
- 26. The method according to claim 19, wherein the monomer is polymerized by emulsion polymerization.
- 27. The method according to claim 21, wherein the polymer is formed from a monomer that is polymerized by suspension polymerization.
- 28. The method according to claim 21, wherein the polymer is formed from a monomer that is polymerized by emulsion polymerization.
Parent Case Info
This application is a continuation-in-part of U.S. Ser. No. 09/336,968, filed Jun. 21, 1999 and now abandoned, which is a continuation of U.S. Ser. No. 08/947,747, filed Oct. 9, 1997, now U.S. Pat. No. 5,914,367. Each of the above applications are hereby incorporated by reference. This application also claims priority to provisional application 60/028,564, filed Oct. 10, 1996, the entirety of which is hereby incorporated by reference.
Government Interests
This invention was made with Government support under Grant No. 59-3K95-3-126 awarded by the U.S. Department of Agriculture, Agricultural Research Service. The Government has certain rights in the invention.
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Provisional Applications (1)
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Number |
Date |
Country |
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60/028564 |
Oct 1996 |
US |
Continuations (1)
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Number |
Date |
Country |
Parent |
08/947747 |
Oct 1997 |
US |
Child |
09/336968 |
|
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09/336968 |
Jun 1999 |
US |
Child |
09/437495 |
|
US |