Patent Application
20210205816
The present invention relates to a polymerase chain reaction apparatus and a polymerase chain reaction method using the same, and more particularly, to a polymerase chain reaction apparatus which is rapid, simple, and easily used and has high portability to allow point-of-care, and a polymerase chain reaction method using the same.
Molecular diagnosis plays an important role in various fields ranging from medical fields to forensic fields and the like by analyzing an intrinsic biomarker (DNA, RNA, protein, and the like). Recently, in the molecular diagnosis fields, a study of a molecular diagnosis method for point-of-care (POC) which allows a timely diagnosis and personalized treatment of various diseases including infectious diseases, cancer, and the like, has been actively conducted. In order to use a molecular diagnosis method for point-of-care, a diagnostic technology should have ASSURED criteria such as being affordable, small, simple, user-friendly, rapid & robust, equipment-free, and disposable.
A polymerase chain reaction (PCR) is a technology to amplify nucleic acids and a core technology which is essentially used in a molecular biological diagnostic method. Specifically, PCR is a method using a characteristic of replicating DNA by a DNA polymerase, and the DNA polymerase may synthesize complementary DNA using single stranded DNA as a template and the single stranded DNA may be simply obtained by boiling double stranded DNA. This process is referred to as DNA denaturation. In order for the DNA polymerase to start DNA replication, a start site should be in the form of double stranded DNA. Therefore, in PCR, small DNA pieces, which may be complementarily annealed to template DNA at both ends of a DNA sequence to be amplified, should be added together, and this should be annealed to both ends of a certain DNA sequence to become double stranded DNA. Only in this case, DNA replication by a DNA polymerase may start. A small DAN piece which may be complementarily annealed to the template DNA end is referred to as an oligonucleotide primer or a primer as an abbreviation. After the primer is annealed to the end of the template DNA once, DNA synthesis is extended to the end of the opposite side by the action of the DNA polymerase.
Since a PCR reaction is based on the mechanism described above, a PCR cycle is composed of three steps of a denaturation step of changing double stranded template DNA into single stranded DNA, an annealing step of annealing a primer to the end of template DNA, and an elongation step of extending DNA synthesis to the end of the opposite by the action of a DNA polymerase. After one PCR cycle is finished, in the next PCR cycle, original template DNA and newly synthesized DNA by PCR all become DNA templates. As such, as PCR cycles are continuously repeated, the number of DAN templates is rapidly increased.
Three steps of denaturation-annealing-elongation are controlled by temperature, and thus, a PCR cycle may correspond to a temperature cycle of denaturation temperature-annealing temperature-elongation temperature. An instrument for PCR to form the temperature cycle is generally referred to as a thermal cycler.
A conventional instrument or thermal cycler for PCR adjusts a temperature of a metal plate to form a temperature cycle based on a Peltier element. The conventional thermal cycle for PCR is widely used in most laboratories and hospitals. However, since the thermal cycler is voluminous and has high power consumption, and it takes a long time to obtain a result, it is inappropriate to be utilized in molecular diagnosis for point-of-care in which diagnosis should be performed in real time.
After a first generation PCR method, various PCR technologies have been studied. For example, a method of increasing a sample surface area exposed to heat using capillaries, a method of using a micro-fluid substrate (Korean Patent Registration No. 10-0756874), a method of using water droplets and a laser, and the like have been reported. However, newly developed PCR technologies also have limitations in direct use in the molecular diagnosis for point-of-care, for example, low portability and decreased amplification efficiency, and requires a treatment by a trained technician, or since it is too expensive to be used for a single use, professional maintenance of the apparatus is required.
An object of the present invention is to provide a polymerase chain reaction apparatus which may be used in molecular diagnosis for point-of-care and a polymerase chain reaction method using the same.
Another object of the present invention is to provide an ultrafast polymerase chain reaction apparatus and a polymerase chain reaction method using the same.
Still another object of the present invention is to provide a polymerase chain reaction apparatus which allows an amplification degree of a material by a polymerase chain reaction (such as nucleic acid) to be observed in real time, and a polymerase chain reaction method using the same.
In one general aspect, a polymerase chain reaction apparatus includes a transparent photothermal substrate including a transparent plate on which an array of transparent nanopillars arranged to be spaced apart from each other is formed and plasmonic metal nanoislands positioned on surfaces including an upper surface and a side surface of the nanopillars; a light source which is positioned on a lower portion of the photothermal substrate and irradiates the plasmonic metal nanoislands with a light; and a chamber containing a fluid heated by the transparent photothermal substrate.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the plasmonic metal nanoislands may be positioned to be spaced apart from each other.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, an average diameter of the nanopillars may be 50 to 1000 nm, and an aspect ratio obtained by dividing an average length of the nanopillars by the average diameter of the nanopillar may be 0.1 to 10.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, a coverage which is, based on one surface of the transparent plate on which the array of nanopillars is positioned, a ratio of an area of the one surface covered by the array of nanopillars to an area of the one surface, may be 0.1 to 0.9.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the nanoislands positioned on the upper surface of the nanopillars may have a shape corresponding to the upper surface of the nanopillars, and the nanoislands positioned on the side surface of the nanopillars may have an average diameter of 5 to 100 nm.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the photothermal substrate may further include a heat dissipation layer which covers nanopillars on which the plasmonic metal nanoislands are positioned.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the light from the light source may be in a visible light to a near-infrared ray.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the chamber may have inner space comparted by a lower surface and a side surface and may contain the fluid in the inner space.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the chamber is positioned to be in contact with the metal nanoislands positioned on the upper surface of the nanopillars but is extended from an upper plate covering the array of nanopillars so as to surround the upper plate and the array of nanopillars, whereby one extended end may include a side portion in contact with the transparent plate.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the polymerase chain reaction apparatus may further include a temperature sensor for measuring a temperature of a space containing a fluid in the chamber or the photothermal substrate.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the polymerase chain reaction apparatus may further include a light irradiation control unit for controlling the light source and the light irradiation control unit may control one or more factors selected from a light intensity, a light irradiation time, and a light irradiation cycle.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the light irradiation control unit may control the light source so as to conform to a preset temperature profile.
In the polymerase chain reaction apparatus according to an exemplary embodiment of the present invention, the polymerase chain reaction apparatus may further include a detector for detecting a Raman signal generated from the fluid.
The present invention includes a polymerase chain reaction method using the polymerase chain reaction apparatus described above.
In another general aspect, the polymerase chain reaction method using the polymerase chain reaction apparatus described above includes: a) adding a fluid which is subject to a polymerase chain reaction to a chamber; and b) irradiating the transparent photothermal substrate with a light generated from a light source so that the fluid conforms to a temperature profile over time.
In the polymerase chain reaction method according to an exemplary embodiment of the present invention, one or more factors selected from a light intensity irradiated on the transparent photothermal substrate, a light irradiation time, and a light irradiation cycle may be controlled to satisfy the temperature profile over time.
In the polymerase chain reaction method according to an exemplary embodiment of the present invention, the temperature profile over time may be a profile in which a temperature rise from an annealing temperature to a denaturation temperature and a temperature reduction from a denaturation temperature to an annealing temperature are set to a cycle and the cycle is repeated.
The polymerase chain reaction method according to an exemplary embodiment of the present invention may further include: in the middle of the step b) or after the step b), irradiating the fluid with an excitation light for Raman scattering or the light from the light source to perform surface enhanced Raman scattering analysis.
The polymerase chain reaction apparatus according to the present invention converts light energy into thermal energy, based on a plasmonic photothermal effect by a transparent photothermal substrate including a transparent plate on which an array of transparent nanopillars arranged to be spaced apart from each other and plasmonic metal nanoislands positioned on surfaces including an upper surface and a side surface of the nanopillars, thereby having a high absorbance and a high-efficiency photothermal effect in a visible light region, and allows ultrafast temperature change and an ultrafast polymerase chain reaction thereby to more rapidly and efficiently amplify nucleic acids (such as DNA, RNA, and cDNA) than a conventional polymerase chain reaction technology. In addition, the polymerase chain reaction apparatus according to the present invention may be manufactured at low costs to have excellent commerciality and also have portability to be used as a molecular diagnostic tool for point-of-care as well as molecular diagnosis at a laboratory level.
In addition, in the polymerase chain reaction apparatus according to the present invention, a transparent photothermal substrate generating a plasmonic photothermal effect includes a transparent plate on which an array of transparent nanopillars are arranged to be spaced apart from each other and plasmonic metal nanoislands positioned on surfaces including an upper surface and a side surface of the nanopillars to have a surface enhanced Raman scattering activity, thereby allowing an amplification degree of nucleic acids to be measured in real time, together with amplification of nucleic acids.
Hereinafter, the polymerase chain reaction apparatus of the present invention and the polymerase chain reaction method using the same will be described in detail, with reference to the attached drawings. The drawings to be provided below are provided by way of example so that the idea of the present invention can be sufficiently transferred to a person skilled in the art to which the present invention pertains. Therefore, the present invention is not limited to the drawings provided below but may be embodied in many different forms, and the drawings suggested below may be exaggerated in order to clear the spirit of the present invention. Technical terms and scientific terms used herein have the general meaning understood by those skilled in the art to which the present invention pertains unless otherwise defined, and a description for the known function and configuration which may unnecessarily obscure the gist of the present invention will be omitted in the following description and the accompanying drawings.
The PCR apparatus according to the present invention includes a transparent photothermal substrate including a transparent plate on which an array of transparent nanopillars arranged to be spaced apart from each other is formed and plasmonic metal nanoislands positioned on surfaces including an upper surface and a side surface of the nanopillars; a light source which is positioned on a lower portion of the photothermal substrate and irradiates the plasmonic metal nanoislands with a light; and a chamber containing a fluid heated by the transparent photothermal substrate.
The PCR apparatus according to the present invention is a PCR apparatus based on a plasmonic photothermal effect in which nanoislands of a plasmonic metal which is a metal having a plasmonic activity are positioned on surfaces including an upper surface and a side surface of each transparent nanopillar forming an array of transparent nanopillars, thereby strongly absorbing light energy by plasmonic metal nanoislands and emitting the absorbed light energy as thermal energy.
In addition, on the transparent photothermal substrate 1000, metal nanoislands 230 may be positioned also on a surface of the transparent plate between the nanopillars, together with the metal nanoislands 210 positioned on the upper surface of each nanopillar 110 and the metal nanoislands 220 positioned on the side surface of each nanopillar 110, as in an example illustrated in
That is, on the transparent photothermal substrate 1000, the plasmonic metal nanoislands 210, 220, and 230 may be positioned on an area including the upper surface and the side surface of each nanopillar 110 forming the array of transparent nanopillars and the surface of the transparent plate exposed between the nanopillars 110.
Here, the nanoisland of the metal islands 210, 220, and 230 may mean a state in which the plasmonic metal positioned on the transparent photothermal substrate 1000 is not connected to each other to form a continuum. Here, a standard of the continuum may be determined by whether the plasmonic metal is continuously connected from the upper surface (surface of one end) of a pillar to a lower end (the other end) of the pillar, in a pillar length direction of the nanopillars. In other words, the plasmonic metal nanoislands may be in a state of being positioned to be spaced apart from each other, and be randomly positioned but be in a state of being spaced apart from each other.
Absorption of light energy by the plasmonic metal nanoislands may include absorption of light energy by surface plasmon resonance (SPR) of the metal nanoislands 210, 220, and 230 positioned on the transparent photothermal substrate 1000, localized surface plasmon resonance (LSPR) generated in hot-spots between the metal nanoislands 220 or between the metal nanoislands 210 and the metal nanoislands 220 positioned on one nanopillar 110, and localized surface plasmon resonance (LSPR) generated in hot-spots between the metal nanoislands 210 and 220 positioned on one nanopillar and the metal nanoislands 210 and 220 positioned on another nanopillar in nanopillars adjacent to each other.
The hot-spots between the metal nanoislands may include hot-spots between the metal nanoislands 210 positioned on the upper surface of the nanopillars adjacent to each other, and therewith or independently thereof, the hot-spots between the metal nanoislands may include hot-spots between the metal nanoislands positioned on the side surface of the nanopillars adjacent to each other (between the metal nanoislands 210 belonging to nanopillars 110 adjacent to each other).
Furthermore, the hot-spots between the metal nanoislands may include hot-spots between the metal nanoislands 230 positioned on the surface of the transparent plate, and therewith or independently thereof, between the metal nanoisland 220 positioned on the side surface of one nanopillar 110 and the metal nanoisland 230 positioned on the surface of the transparent plate.
Specifically, the transparent photothermal substrate of
As seen from
However, it was found that the Au thin film represents a light absorbance of 0.5 or more in a region of 400-500 mm where absorption by the plasmonic characteristic of Au occurs, but as the light wavelength is increased to more than 500 nm, a light absorbance is rapidly decreased and the light absorbance at a wavelength of 600 nm was less than 0.1.
Since the transparent photothermal substrate according to an exemplary embodiment of the present invention has an excellent light absorbance of 0.5 or more to light in the visible light to near-infrared ray region, in particular, the entire visible light region (400-700 nm), a photothermal effect may be generated by simple visible light irradiation which does not cause optical damage to a biochemical material including nucleic acids, and light energy is absorbed with an extremely excellent light absorbance in the entire wavelength region of 400 to 700 nm, and thus, higher thermal energy may be generated in such case than in the case in which the same size of light energy is irradiated.
In the PCR apparatus according to an exemplary embodiment of the present invention, being “transparent” may mean having a transmittance of light, specifically a visible light (400-700 nm) to a near-infrared ray (0.78-3 μm), specifically visible light of 90% or more, more specifically 95% or more, and still more specifically 98% or more.
The transparent plate may be any material as long as it is an insulating material transparent to light, specifically a light in a visible light to a near-infrared ray, and more specifically a visible light. As a substantial example, the transparent plate may be transparent inorganic materials such as glass, transparent polymers such as polymethylmethacrylate (PMMA), polydimethylsiloxane (PDMS), and polyurethane acrylate (PUA), and the like, but is not limited thereto.
The transparent nanopillar may be the same or different materials as/from the transparent plate, and may be any material as long as it is an insulating material transparent to light, specifically a light in a visible light to a near-infrared ray, and more specifically a visible light. As a substantial example, the nanopillars may be transparent inorganic materials such as glass, transparent polymers such as polymethylmethacrylate (PMMA), polydimethylsiloxane (PDMS), and polyurethane acrylate (PUA), and the like, but are not limited thereto.
An average diameter of the nanopillars forming the array of nanopillars may be 50 to 1000 nm, specifically 100 to 500 nm, and more specifically 100 to 300 nm, and an aspect ratio obtained by dividing an average length of the nanopillars by the average diameter may be 0.1 to 10, specifically 1 to 10, more specifically 1.5 to 7, and still more specifically 1.5 to 4. The average diameter and the aspect ratio of the nanopillars are the size and the aspect ratio at which even the same kind of LSPR may form various LSPR wavelengths by arrangement of metal nanoislands having a large size distribution and irregular (random) metal nanoislands to show a high light absorbance of 0.55 or more in the entire wavelength region of 400-700 nm, and simultaneously, are the size and the aspect ratio at which excellent mechanical (physical) stability may be secured, a large amount of heat may be generated by a photothermal effect, and the generated heat may be rapidly conducted to a fluid, but the present invention is not necessarily limited thereto.
A coverage which is a ratio of an area of one surface covered with the array of nanopillars to an area of one surface, based on the one surface of the transparent plate on which the array of nanopillars, may be 0.10 to 0.90, specifically 0.40 to 0.85, and more specifically 0.50 to 0.85.
A ratio of an area (coverage) of one surface of the transparent plate covered with the array of nanopillars affects heat generated per a unit area of the transparent photothermal substrate, and the coverage described above is a coverage at which uniform heating or temperature change of a fluid may occur even in the transparent photothermal substrate having a large area up to 4 inches, but the present invention is not necessarily limited thereto.
The metal of the metal nanoislands may be any metal having a plasmon activity. As a specific example, the metal of the metal nanoislands may be noble metals such as gold, platinum, and silver, copper, nickel, aluminum, and the like, and may be gold in terms of biocompatibility, but the present invention is not necessarily limited thereto.
In the transparent photothermal substrate, the nanoislands (210 of
In addition, the nanoislands 220 positioned on the side surface of the nanopillars or the nanoislands 230 positioned on the surface of the transparent plate between the nanopillars may have a circular shape, an ellipsoidal shape, or an irregular shape, and the metal nanoislands 220 and 230 may have an average diameter of 5 to 100 nm. As a specific example, the metal nanoislands 220 and 230 may have an average diameter of 5 to 100 nm, specifically 5 to 50 nm, and more specifically 5 to 30 nm, and an average of 4 to 10 metal nanoislands may be positioned to be spaced apart in a length direction of nanopillars.
As seen from
The plasmonic metal nanoislands 210, 220, and 230 may have a truncated particle shape, specifically a truncated spherical shape, and the truncated part may form an interface with the nanopillars 110 or the transparent plate 100.
The light from the light source may be in a visible light to a near-infrared ray. The visible light may be a light belonging to a region of 400 to 700 nm and the near-infrared ray may be a light belonging to a region of 0.78 to 3 μm. For a high light absorbance, prevention of damage to a biochemical material including nucleic acids, and generation of a more rapid and uniform photothermal effect, the irradiated light from the light source is advantageously a visible light. Here, the visible light does not mean only a white light, and may include a red light, a green light, a blue light, or a combination thereof, of course. That is, the light source may include a combination including a red light source, a green light source, a blue light source, or a white light source.
However, as described above, the transparent photothermal substrate 1000 according to a specific example of the present invention may have an extremely high light absorbance of 0.5, substantially 0.55 or more in the entire visible light region (400-700 nm). Thus, a light having a single wavelength may be irradiated, but the light source is advantageously a white light source so that more heat may be generated in a shorter time. Substantially, the white light source may be a halogen lamp, a xenon lamp, a white LED, or the like, but is not necessarily limited thereto. Here, if necessary, a white light (visible light) from which ultraviolet rays and the like generated from the light source are removed may be irradiated using a filter or the like, of course.
In addition, as in an example illustrated in
In an example of
The fluid (fluid for PCR) may be a solution including a target nucleic acid, a nucleic acid polymerase, and a primer pair to amplify the target nucleic acid (including a buffer solution), but is not necessarily limited thereto, and may be any common composition used in PCR. The target nucleic acid means all nucleic acids to be amplified by an amplification reaction of PCR and the like, and as an example, may be nucleic acids such as DNA, RNA, and cDNA containing a single or a plurality of nucleotide polymorphisms.
The heat dissipation layer 300 may serve to more rapidly transfer heat generated by the plasmonic photothermal effect to the chamber, and also, may serve to neutralize surface charges.
The heat dissipation layer 300 may be any material having a similar or higher value of thermal conductivity as compared with the thermal conductivity of the transparent plate. As a specific example, the heat dissipation layer 300 may be inorganic materials such as a-Si, SiO2, and hydrogen silsesquioxane (HSQ), polymers such as polydimethylsiloxane (PDMS), and the like, and may have a thickness of 1 nm to 100 μm, but is not limited thereto. In addition, in
An empty space between the nanopillars 110 on which the metal nanoislands are formed may correspond to the inner space containing the fluid in the chamber 3000, and also, the chamber 3000 may be further provided with an inlet for injecting the fluid and a through hole for venting in an edge area.
Since the empty space between the nanopillars 110 becomes the space containing the fluid, the metal nanoislands generating the plasmonic photothermal effect may be brought into direct contact with the fluid. Thus, heat generated by light may directly heat the fluid and the temperature of the fluid may be controlled more rapidly and uniformly, and also, the temperature may be controlled so that the fluid injected by light control very accurately conforms to the temperature profile set as a whole.
The material of the chamber 3000 illustrated in an example of
The polymerase chain reaction apparatus according to an exemplary embodiment of the present invention may further include a temperature sensor for measuring the temperature of the space containing the fluid in the photothermal substrate or the chamber. The temperature sensor may be any apparatus to measure the temperature of the housing space in the chamber, such as a thermographic camera and a thermocouple. The temperature sensor may be unnecessary when PCR is in progress, but may be required when the values of control factors of the light source are set so as to conform to the set temperature profile.
The polymerase chain reaction apparatus according to an exemplary embodiment of the present invention may further include a light irradiation control unit for controlling the light source, and the light irradiation control unit may control one or more factors selected from a light intensity, a light irradiation time, and a light irradiation cycle.
Specifically, the light irradiation control unit may control the light source so as to conform to the preset temperature profile, and may control one or more factors selected from a light intensity, a light irradiation time, and a light irradiation cycle, so as to conform to the preset temperature profile. Here, the light irradiation control unit may control at least one or more factors selected from a light intensity, a light irradiation time, and a light irradiation cycle, so as to conform to the preset temperature profile based on information of the temperature sensor input by output of the temperature sensor.
The control of the light irradiation time may include control of irradiation forms such as pulse irradiation or continuous irradiation. Control of a light irradiation cycle may include on/off control of light, and pulse irradiation may include control of a pulse width and an interval between pulses. The light intensity may include a light intensity over time (constant or changed intensity), and the pulse irradiation may include control of a maximum value (intensity) and a minimum value (intensity) (including 0) forming the pulse. The light intensity may be controlled by voltage, current, and the like applied to the light source, of course.
The temperature profile over time is a preset temperature profile, and may be in a state in which controlled values of one or more factors selected from a light intensity, a light irradiation time, and a light irradiation cycle has been previously input to the light irradiation control unit so as to conform to the corresponding temperature profile by the previous experiment. On the other hand, one or more factors selected from a light intensity, a light irradiation time, and a light irradiation cycle may be controlled so as to conform to the temperature profile preset by inputting the output of the temperature sensor.
An example of the temperature profile over time which has been previously input to the control unit may be a profile in which a temperature rise from an annealing temperature to a denaturation temperature and a temperature reduction from a denaturation temperature to an annealing temperature are set to a cycle and the cycle is repeated and a temperature profile in which a temperature rise rate value, a temperature reduction rate value, an annealing temperature value, a denaturation temperature value, a holding time at the annealing temperature (including 0), a holding time at the denaturation temperature, the number of repeated cycles, and the like are predetermined, but the present invention may not be limited to the specific conditions of the temperature profile, of course.
The polymerase chain reaction apparatus according to an exemplary embodiment of the present invention may further include a detector for detecting a Raman signal generated from the fluid. That is, by further including the detector for detecting a Raman signal, material information of nucleic acids amplified by a surface enhanced Raman scattering (SERS) spectroscopy and an amplification degree may be analyzed in real time. Here, when the light source includes the wavelength of an excitation light for Raman scattering, separate laser (laser generating a light of an excitation wavelength) is not needed, but when selectively required, laser generating a light of an excitation wavelength may be further included together with the detector.
The SERS analysis in real time which may be performed together with nucleic acid amplification is based on the facts that the transparent photothermal substrate described above is a substrate provided with hot-spots which may enhance a Raman signal, the wavelength of LSPR present in the transparent photothermal substrate is present in a large wavelength region from 400 to 700 nm, and in particular, as in the chamber of
Here, when SERS analysis is intended to be performed at the same time, the fluid may further include a Raman prove bound to a target nucleic acid, together with the target nucleic acid, a nucleic acid polymerase, and a primer pair to amplify the target nucleic acid, of course.
The SERS analysis may be performed in real time in the middle of nucleic acid amplification, or otherwise, may be performed after nucleic acid amplification has been completed.
As seen from
As seen from the results of an Au film of
Specifically, the result of
An amplified DNA intensity in
As seen from
As seen from
In the results of
The present invention includes a PCR method using the PCR apparatus described above.
The PCR method according to the present invention is a polymerase chain reaction method using the polymerase chain reaction apparatus described above, and includes: a) adding a fluid which is subject to a polymerase chain reaction (fluid for PCR) to a chamber; and b) irradiating the transparent photothermal substrate with a light generated from a light source so that the fluid conforms to a temperature profile over time.
As described above based on the PCR apparatus, the temperature of the fluid may be controlled so as to conform to the preset temperature profile over time by controlling one or more factors selected from a light intensity irradiated on the transparent photothermal substrate, a light irradiation time, and a light irradiation cycle.
Here, the temperature profile over time may be a profile in which a temperature rise from an annealing temperature to a denaturation temperature and a temperature reduction from a denaturation temperature to an annealing temperature are set to a cycle and the cycle is repeated. As a specific example, the annealing temperature may be 50 to 60° C. and the denaturation temperature may be 90 to 98° C., but the temperature profile may be configured with a known annealing temperature and a known denaturation temperature depending on the target nucleic acid which is a nucleic acid to be amplified, a nucleic acid polymerase, and the specific material of the primer pair to amplify the target nucleic acid, of course.
In the middle of the step b) or after the step b), a step of irradiating fluid with an excitation light for Raman scattering or the light from the light source to perform surface enhanced Raman scattering analysis, may be further included. That is, in the PCR method according to an exemplary embodiment of the present invention, nucleic acid detection in real time (nucleic acid detection in real time during an amplification process) using SERS may be simultaneously performed, together with ultrafast and ultrahigh-efficiency nucleic acid amplification.
Hereinabove, although the present invention has been described by specific matters, limited exemplary embodiments, and drawings, they have been provided only for assisting the entire understanding of the present invention, and the present invention is not limited to the exemplary embodiments, and various modifications and changes may be made by those skilled in the art to which the present invention pertains from the description.
Therefore, the spirit of the present invention should not be limited to the above-described exemplary embodiments, and the following claims as well as all modified equally or equivalently to the claims are intended to fall within the scope and spirit of the invention.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2018-0062365 | May 2018 | KR | national |
| 10-2019-0019825 | Feb 2019 | KR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/KR2019/006420 | 5/29/2019 | WO | 00 |
