Claims
- 1. In a process of ion-exchange chromatography, the improvement comprising using as a stationary phase a base polymer selected from the group consisting of a crosslinked styrene polymer or copolymer, or a crosslinked dextran, in which chains of the base polymer or copolymer are substituted with one or more groups, which may be identical or different, belonging to the following categories:
- --Z--A.sub.1 ;
- --Z--A.sub.2 ;
- --Z--A.sub.1 --Z'--A.sub.2 ;
- --Z--A.sub.1 --A.sub.3 --A.sub.2 ;
- --Z--A.sub.1 --A.sub.4
- wherein
- Z and Z' are chosen from the following moieties;
- --(CH.sub.2)--.sub.n, n being 1 to 12, optionally made hydrophilic by the replacement of at least one H by an OH; or
- --O--(CH.sub.2)--.sub.r, r being 0 to 12, optionally being made hydrophilic by the replacement of at least one H by an OH; or
- ----SO.sub.2 NH--(CH.sub.2)--.sub.m, m being 1 to 12, the moiety --(CH.sub.2).sub.m --, optionally being made hydrophilic by the replacement of at least one H by an OH; and in the case of the modification of a crosslinked dextran, also ##STR59## q being 1 to 12, the residue --(CH.sub.2)q-- optionally being made hydrophilic by the replacement of at least one H by an OH;
- A.sub.1 denotes a phosphorylated moiety
- A.sub.2 denotes a chemical moiety derived from a purine base or a pyrimidine base;
- A.sub.3 denotes a chemical group derived from a sugar; and
- A.sub.4 denotes a moiety of a molecule participating in the polar structure of a phospholipid.
- 2. The process of claim 1 wherein enzymes, coenzymes or enzyme complexes accepting DNA, RNA and nucleotides as substituents are purified.
- 3. The process of claim 1 wherein plasma extracts of blood coagulation factors are purified.
- 4. The process of claim 1 wherein proteins participating in blood coagulation are purified.
- 5. The process of claim 1 wherein different types of lupous antibodies are determined.
- 6. The process of claim 1 wherein different types of lupous antibodies are determined.
- 7. In a process of affinity chromatography, the improvement comprising using as a stationary phase a base polymer selected from the group consisting of a crosslinked styrene polymer or copolymer, or a crosslinked dextran, in which chains of the base polymer or copolymer are substituted with one or more groups, which may be identical or different, belonging to the following categories:
- --Z--A.sub.1 ;
- --Z--A.sub.2 ;
- --Z--A.sub.1 --Z'--A.sub.2 ;
- --Z--A.sub.1 --A.sub.3 --A.sub.2 ;
- --Z--A.sub.1 --A.sub.4
- wherein
- Z and Z' are chosen from the following moieties;
- --(CH.sub.2)--.sub.n, n being 1 to 12, optionally made hydrophilic by the replacement of at least one H by an OH; or
- --O--(CH.sub.2)--.sub.r, r being 0 to 12, optionally being made hydrophilic by the replacement of at least on H by an OH; or
- ----SO.sub.2 NH--(CH.sub.2).sub.m --, m being 1 to 12, moiety --(CH.sub.2).sub.m --optionally being made hydrophilic by the replacement of at least one H by an OH; and in the case of the modification of a crosslinked dextran, also ##STR60## q being 1 to 12, the residue --(CH.sub.2)q-- optionally being made hydrophilic by the replacement of at least one H by an OH;
- A.sub.1 denotes a phosphorylated moiety
- A.sub.2 denotes a chemical moiety derived from a purine base or a pyrimidine base;
- A.sub.3 denotes a chemical group derived from a sugar; and
- A.sub.4 denotes a moiety of a molecule participating in the polar structure of a phospholipid.
- 8. The process of claim 7 wherein different types of antibodies developed by lupous patients are selectively purified.
- 9. The process of claim 7 wherein enzymes, coenzymes or enzyme complexes accepting DNA, RNA and nucleotides as substituents are purified.
- 10. The process of claim 7 wherein plasma extracts of blood coagulation factors are purified.
- 11. The process of claim 7 wherein proteins participating in blood coagulation are purified.
- 12. The process of claim 7 wherein different types of circulating anticoagulants and/or antiphosolipids are determined.
Priority Claims (1)
Number |
Date |
Country |
Kind |
87 11813 |
Aug 1987 |
FRX |
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PRIOR APPLICATION
This appliction is a division of U.S. patent application Ser. No. 235,113 filed Aug. 22, 1988, now U.S. Pat. No. 4,950,712.
US Referenced Citations (2)
Divisions (1)
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Number |
Date |
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Parent |
235113 |
Aug 1988 |
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