Claims
- 1. A composition for separating polynucleotides by capillary electrophoresis, the composition comprising:
- a charge-carrying component;
- a sieving component;
- a surface interaction component consisting of one or more uncharged water-soluble silica-adsorbing polymers having (i) water solubility in a temperature range between about 20.degree. C. and about 50.degree. C., (ii) a concentration in the separation medium in a range between about 0.001% and about 10% (weight/volume), (iii) a molecular weight in the range between about 5.times.10.sup.3 and about 1.times.10.sup.6 Daltons, and (iv) an absence of charged groups in an aqueous medium having a pH in the range between about 6 and about 9; and
- the composition having a viscosity of less than about 5000 centipoise.
- 2. The composition of claim 1 having a viscosity less than about 1000 centipoise.
- 3. The composition of claim 1 wherein the sieving component is the same as the surface interaction component.
- 4. The composition of claim 1 wherein the sieving component is an uncrosslinked polymer.
- 5. The composition of claim 4 wherein the uncrosslinked polymer is a linear polymer.
- 6. The composition of claim 1 wherein the surface interaction component, when present at a concentration of at least 0.1% (w:v), is sufficient to reduce electroosmotic flow by a factor of at least eight.
- 7. The composition of claim 1 wherein the surface interaction component, when present at a concentration of at least 0.1% (w:v), is sufficient to reduce electroosmotic flow by a factor of at least 20.
- 8. The composition of claim 1 wherein the surface interaction component, when present at a concentration of at least 0.01% (w:v), is sufficient to reduce electroosmotic flow by a factor of at least 30.
RELATED U.S. APPLICATIONS
This is a continuation of applications Ser. No. 08/637,057, filed on Apr. 24, 1996, pending; which is a continuation of Ser. No. 08/458,525, filed on Jun. 7, 1995 and issued as U.S. Pat. No. 5,552,028 on Sep. 3, 1996; which is a divisional of Ser. No. 08/350,852, filed on Dec. 6, 1994 and issued as U.S. Pat. No. 5,567,292 on Oct. 22, 1996; which is a continuation-in-part of Ser. No. 08/170,078, filed on Dec. 17, 1993, now abandoned, which applications are incorporated herein by reference and to which applications priority is claimed under 35 USC .sctn.120.
US Referenced Citations (6)
Divisions (1)
|
Number |
Date |
Country |
Parent |
350852 |
Dec 1994 |
|
Continuations (2)
|
Number |
Date |
Country |
Parent |
637057 |
Apr 1996 |
|
Parent |
458525 |
Jun 1995 |
|
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
170078 |
Dec 1993 |
|