Claims
- 1. A polynucleotide construct comprising:
a promoter comprising at least nucleotides 1187-1254 of SEQ ID No.:7; and a polynucleotide sequence that encodes a polypeptide of interest.
- 2. A vector comprising the construct of claim 1.
- 3. The vector of claim 2 wherein the vector is a Ti plasmid.
- 4. The vector of claim 3 further comprising:
a T-DNA left border sequence; and a T-DNA right border sequence.
- 5. A cell comprising the construct of claim 1.
- 6. A prokaryotic cell comprising the construct of claim 1.
- 7. A bacterial cell comprising the construct of claim 1.
- 8. A eukaryotic cell comprising the construct of claim 1.
- 9. A plant cell comprising the construct of claim 1.
- 10. A polynucleotide construct within a eukaryotic host cell, comprising:
a polynucleotide sequence encoding a protein other than a phosphoglycerate kinase; and a phosphoglycerate kinase gene promoter sequence isolated from a first species, the first species belonging to a first phylogenetic kingdom, the promoter sequence being operably linked to the polynucleotide sequence, the eukaryotic host cell belonging to a second species within a second phylogenetic kingdom that is different from the first phylogenetic kingdom.
- 11. The polynucleotide construct of claim 10 wherein the first phylogenetic kingdom is fungi.
- 12. The polynucleotide construct of claim 10 wherein the second phylogenetic kingdom is plantae.
- 13. The polynucleotide construct of claim 10 wherein the promoter sequence comprises at least 100 nucleotides of SEQ ID NO.:7.
- 14. The polynucleotide construct of claim 10 wherein the protein confers a resistance to the host cell.
- 15. The polynucleotide construct of claim 14 wherein the resistance is a resistance to at least one of an antibiotic, a herbicide, a pathogen, a toxin and a parasite.
- 16. The polynucleotide construct of claim 10 wherein the polynucleotide construct is integrated into the genomic DNA of the eukaryotic host cell.
- 17. A method of expressing a polypeptide in a host cell comprising:
providing a polynucleotide construct having a polynucleotide encoding a polypeptide of interest operably linked to a fungal phosphoglycerate kinase (pgk) gene promoter sequence; and introducing the polynucleotide construct into a host cell.
- 18. The method of claim 17 wherein the pgk promoter sequence comprises at least 100 nucleotides as set forth in SEQ ID NO.7.
- 19. The method of claim 17 wherein the host cell is a yeast.
- 20. The method of claim 19 wherein the yeast is a species of the genus Saccharomyces.
- 21. The method of claim 17 wherein the host cell is a plant cell.
- 22. The method of claim 21 further comprising growing the plant cell in a cell culture.
- 23. The method of claim 21 further comprising growing the plant cell in plant tissue.
- 24. The method of claim 21 wherein the plant cell is an angiosperm plant cell.
- 25. The method of claim 24 wherein the plant cell is a monocot plant cell.
- 26. The method of claim 25 wherein the plant cell is a maize cell.
- 27. The method of claim 24 wherein the plant cell is a dicot plant cell.
- 28. The method of claim 27 wherein the plant cell is a tobacco plant cell.
- 29. The method of claim 17 wherein the introducing comprises transformation of the host cell utilizing at least one of electroporation, T-DNA mediated transformation, particle-bombardment mediated transformation, microinjection, freeze-thaw transformation, bacterial mediated transformation, virus-mediated transformation, chemical mediated transformation, whiskers mediated transformation, and plasmid transformation.
- 30. A system for producing a polypeptide comprising:
a first polynucleotide sequence isolated from a fungal species; the first polynucleotide sequence comprising a promoter sequence of a phosphoglycerate kinase gene; a second polynucleotide sequence isolated from a second species, the second polynucleotide sequence comprising a coding region that encodes the polypeptide; a host cell containing the first polynucleotide sequence operably linked to the second polynucleotide sequence, the host cell being of a species that is different from the fungal species from which the first polynucleotide sequence is isolated.
- 31. The system of claim 30 wherein the fungal species is from the genus Rhizopus.
- 32. The system of claim 30 wherein the host cell is a plant cell.
- 33. The system of claim 30 wherein the host cell is a yeast cell.
- 34. The system of claim 30 wherein the polypeptide confers a resistance to the host cell.
- 35. A method of producing a protein in a plant cell comprising:
creating a polynucleotide construct by operably linking an isolated fungal 3-phosphoglycerate kinase gene (pgk) promoter sequence to a polynucleotide sequence encoding at least one peptide of interest; transforming the plant cell with the construct; and utilizing the fungal pgk promoter to express the at least one peptide of interest within the plant cell.
- 36. The method of claim 35 wherein the polynucleotide sequence encodes a peptide foreign to the plant cell.
- 37. The method of claim 35 wherein the polynucleotide sequence encodes a peptide native to the plant cell.
- 38. The method of claim 37 wherein a non-transformed plant cell produces the peptide at a first level and the transforming the plant cell increases production of the peptide to a second level.
- 39. A plant cell comprising a promoter sequence isolated from Rhizopus Oryzae.
- 40. The plant cell of claim 39 wherein the promoter sequence comprises at least 100 nucleotides of SEQ ID. No 7.
- 41. The plant cell of claim 39 wherein the promoter sequence comprises the first 1254 nucleotides of SEQ ID No:7.
- 42. The plant cell of claim 39 wherein the promoter sequence is integrated into the plant cell genomic DNA.
- 43. A yeast cell comprising at least one promoter sequence isolated from Rhizopus Oryzae.
- 44. The yeast cell of claim 43 wherein the promoter sequence comprises at least 100 nucleotides of SEQ ID. No 7.
- 45. The yeast cell of claim 43 wherein the promoter sequence comprises the first 1254 nucleotides of SEQ ID No:7
RELATED PATENT DATA
[0001] This application is a continuation-in-part of U.S. application Ser. No. 09/536,034 which was filed on Mar. 27, 2000 and which is hereby incorporated by reference.
CONTRACTUAL ORIGIN OF THE INVENTION
[0002] The U.S. Government has a paid-up license in this invention and the right in limited circumstances to require the patent owner to license others on reasonable terms as provided for by the terms of contract number DE-AC0676RLO1830, awarded by the U.S. Department of Energy.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09536034 |
Mar 2000 |
US |
Child |
10214655 |
Aug 2002 |
US |