Claims
- 1. A purified corn tassel-derived polynucleotide comprising a nucleic acid sequence selected from the Sequence Listing or a complement of the nucleic acid sequence.
- 2. A composition for the detection of altered expression of corn tassel-derived polynucleotides comprising at least one of the nucleic acid sequences of claim 1.
- 3. A corn tassel-derived polynucleotide selected from the following nucleic acid sequences of claim 1: SEQ ID NO: 1615 (700344704H1), SEQ ID NO: 2691 (700346502H1), SEQ ID NO: 2813 (700346702H1), SEQ ID NO: 3616 (700380601H1), SEQ ID NO: 3681 (700380774H1), SEQ ID NO: 233 (700342388H1), SEQ ID NO: 2060 (700345470H1), SEQ ID NO: 964 (700343568H1), SEQ ID NO: 2811 (700346695H1), SEQ ID NO: 2519 (700346233H1), SEQ ID NO: 2083 (700345510H1), SEQ ID NO: 1101 (700343780H1), SEQ ID NO: 1847 (700345052H1), SEQ ID NO: 1777 (700344946H1), SEQ ID NO: 1780 (700344949H1), SEQ ID NO: 1733 (700344870H1), SEQ ID NO: 3018 (700347036H1), SEQ ID NO: 3158 (700347281H1), SEQ ID NO: 2220 (700345749H1), SEQ ID NO: 165 (700342268H1), SEQ ID NO: 139 (700342223H1), SEQ ID NO: 2823 (700346721H1), SEQ ID NO: 2065 (700345477H1), SEQ ID NO: 605 (700343040H1), SEQ ID NO: 3618 (700380603H1), SEQ ID NO: 1537 (700344525H1), SEQ ID NO: 469 (700342790H1), SEQ ID NO: 3344 (700379867H1), SEQ ID NO: 180 (700342291H1), SEQ ID NO: 303 (700342521H1), SEQ ID NO: 1249 (700344013H1), SEQ ID NO: 1036 (700343679H1), SEQ ID NO: 2920 (700346877H1), SEQ ID NO: 3619 (700380604H1), SEQ ID NO: 1061 (700343721H1), SEQ ID NO: 1589 (700344654H1), SEQ ID NO: 3100 (700347193H1), SEQ ID NO: 2182 (700345675H1), SEQ ID NO: 2218 (700345743H1), SEQ ID NO: 398 (700342679H1), SEQ ID NO: 175 (700342283H1), SEQ ID NO: 3533 (700380411H1), SEQ ID NO: 3615 (700380596H1), SEQ ID NO: 89 (700281941H2), SEQ ID NO: 3530 (700380405H1), SEQ ID NO: 3023 (700347043H1), SEQ ID NO: 3185 (700347339H1), SEQ ID NO: 3062 (700347112H1), SEQ ID NO: 1082 (700343752H1), SEQ ID NO: 55 (700281888H2), SEQ ID NO: 2955 (700346930H1), SEQ ID NO: 1032 (700343674H1), SEQ ID NO: 163 (700342263H1), SEQ ID NO: 2864 (700346807H1), SEQ ID NO: 1286 (700344069H1), SEQ ID NO: 2560 (700346291H1), SEQ ID NO: 742 (700343247H1), SEQ ID NO: 1731 (700344866H1), SEQ ID NO: 370 (700342630H1), SEQ ID NO: 1167 (700343872H1), SEQ ID NO: 715 (700343202H1), SEQ ID NO: 3314 (700379793H1), SEQ ID NO: 2156 (700345638H1), SEQ ID NO: 624 (700343071H1), SEQ ID NO: 545 (700342924H1), SEQ ID NO: 2855 (700346794H1), SEQ ID NO: 850 (700343408H1), SEQ ID NO: 1557 (700344614H1), SEQ ID NO: 1872 (700345114H1), SEQ ID NO: 1000 (700343626H1), SEQ ID NO: 1045 (700343695H1), SEQ ID NO: 2753 (700346604H1), SEQ ID NO: 70 (700281910H1), SEQ ID NO: 1235 (700343988H1), SEQ ID NO: 389 (700342664H1), SEQ ID NO: 486 (700342817H1), SEQ ID NO: 766 (700343281H1), SEQ ID NO: 1310 (700344105H1), SEQ ID NO: 3202 (700347368H1), SEQ ID NO: 1941 (700345253H1), SEQ ID NO: 6 (700281814H2), SEQ ID NO: 1884 (700345138H1), SEQ ID NO: 2316 (700345916H1), SEQ ID NO: 1948 (700345263H1), SEQ ID NO: 2277 (700345849H1), SEQ ID NO: 2019 (700345394H1), SEQ ID NO: 1696 (700344820H1), SEQ ID NO: 226 (700342373H1), SEQ ID NO: 262 (700342448H1), SEQ ID NO: 1377 (700344208H1), SEQ ID NO: 1279 (700344059H1), SEQ ID NO: 1770 (700344935H1), SEQ ID NO: 532 (700342903H1), SEQ ID NO: 843 (700343396H1), SEQ ID NO: 360 (700342616H1), SEQ ID NO: 540 (700342915H1), SEQ ID NO: 413 (700342708H1), SEQ ID NO: 1252 (700344017H1), SEQ ID NO: 1560 (700344618H1), SEQ ID NO: 3541 (700380432H1), SEQ ID NO: 243 (700342413H1), SEQ ID NO: 1938 (700345249H1), SEQ ID NO: 1127 (700343816H1), SEQ ID NO: 1545 (700344541H1), SEQ ID NO: 1946 (700345258H1), SEQ ID NO: 298 (700342510H1), SEQ ID NO: 2906 (700346860H1), SEQ ID NO: 783 (700343310H1), SEQ ID NO: 616 (700343060H1), SEQ ID NO: 2097 (700345529H1), SEQ ID NO: 2393 (700346036H1), SEQ ID NO: 860 (700343420H1), SEQ ID NO: 2196 (700345705H1), SEQ ID NO: 2403 (700346049H1), SEQ ID NO: 2309 (700345906H1), SEQ ID NO: 1963 (700345289H1), SEQ ID NO: 1383 (700344218H1), SEQ ID NO: 3201 (700347367H1), SEQ ID NO: 3589 (700380530H1), SEQ ID NO: 660 (700343124H1), SEQ ID NO: 2254 (700345807H1), SEQ ID NO: 2689 (700346496H1), SEQ ID NO: 3166 (700347296H1), SEQ ID NO: 2023 (700345404H1), SEQ ID NO: 726 (700343218H1), SEQ ID NO: 363 (700342620H1), SEQ ID NO: 2968 (700346951H1), SEQ ID NO: 2214 (700345737H1), SEQ ID NO: 989 (700343607H1), SEQ ID NO: 2642 (700346426H1), SEQ ID NO: 2741 (700346581H1), SEQ ID NO: 1321 (700344122H1), SEQ ID NO: 1255 (700344022H1), SEQ ID NO: 2311 (700345908H1), SEQ ID NO: 1857 (700345075H1), SEQ ID NO: 2571 (700346316H1), SEQ ID NO: 3063 (700347116H1), SEQ ID NO: 1649 (700344750H1), SEQ ID NO: 1653 (700344754H1), SEQ ID NO: 1762 (700344924H1), SEQ ID NO: 1985 (700345329H1), SEQ ID NO: 2382 (700346023H1), SEQ ID NO: 2468 (700346152H1), SEQ ID NO: 1567 (700344628H1), SEQ ID NO: 991 (700343609H1), SEQ ID NO: 2124 (700345577H1), SEQ ID NO: 829 (700343377H1), SEQ ID NO: 3623 (700380616H1), SEQ ID NO: 725 (700343216H1), SEQ ID NO: 1676 (700344785H1), SEQ ID NO: 399 (700342680H1), SEQ ID NO: 2790 (700346658H1), SEQ ID NO: 2284 (700345859H1), SEQ ID NO: 2838 (700346745H1), SEQ ID NO: 757 (700343266H1), SEQ ID NO: 1251 (700344016H1), SEQ ID NO: 207 (700342338H1), SEQ ID NO: 3515 (700380270H1), SEQ ID NO: 3224 (700379610H1), SEQ ID NO: 2958 (700346935H1), SEQ ID NO: 1861 (700345079H1), SEQ ID NO: 3052 (700347092H1), SEQ ID NO: 2542 (700346264H1), SEQ ID NO: 3602 (700380571H1), SEQ ID NO: 2788 (700346656H1), SEQ ID NO: 1966 (700345293H1), SEQ ID NO: 1813 (700345004H1), SEQ ID NO: 573 (700342986H1), SEQ ID NO: 3572 (700380488H1), SEQ ID NO: 1368 (700344191H1), SEQ ID NO: 774 (700343291H1), SEQ ID NO: 443 (700342747H1), SEQ ID NO: 1083 (700343755H1), SEQ ID NO: 110 (700281973H1), SEQ ID NO: 118 (700281986H2), SEQ ID NO: 2088 (700345516H1), SEQ ID NO: 2932 (700346894H1), SEQ ID NO: 2815 (700346704H1), SEQ ID NO: 1112 (700343793H1), SEQ ID NO: 3131 (700347236H1), SEQ ID NO: 682 (700343154H1), SEQ ID NO: 1965 (700345291H1), SEQ ID NO: 1866 (700345094H1), SEQ ID NO: 898 (700343475H1), SEQ ID NO: 3582 (700380514H1), SEQ ID NO: 916 (700343509H1), SEQ ID NO: 188 (700342309H1), SEQ ID NO: 1364 (700344186H1), SEQ ID NO: 1421 (700344295H1), SEQ ID NO: 3685 (700380786H1), SEQ ID NO: 2404 (700346050H1), SEQ ID NO: 3246 (700379650H1), SEQ ID NO: 1673 (700344782H1), SEQ ID NO: 953 (700343552H1), SEQ ID NO: 2216 (700345739H1), SEQ ID NO: 1232 (700343985H1), SEQ ID NO: 775 (700343292H1), SEQ ID NO: 869 (700343431H1), SEQ ID NO: 145 (700342231H1), SEQ ID NO: 2927 (700346887H1), SEQ ID NO: 1820 (700345013H1), SEQ ID NO: 2244 (700345785H1), SEQ ID NO: 1078 (700343746H1), SEQ ID NO: 2965 (700346943H1), SEQ ID NO: 1085 (700343757H1), SEQ ID NO: 730 (700343225H1), SEQ ID NO: 792 (700343325H1), SEQ ID NO: 3218 (700347395H1), SEQ ID NO: 3033 (700347064H1), SEQ ID NO: 1058 (700343715H1), SEQ ID NO: 302 (700342515H1), SEQ ID NO: 2384 (700346026H1), SEQ ID NO: 1220 (700343965H1), SEQ ID NO: 1883 (700345136H1), SEQ ID NO: 1928 (700345235H1), SEQ ID NO: 2598 (700346351H1), SEQ ID NO: 315 (700342543H1), SEQ ID NO: 1405 (700344265H1), SEQ ID NO: 3128 (700347232H1), SEQ ID NO: 942 (700343540H1), SEQ ID NO: 1109 (700343790H1), SEQ ID NO: 1490 (700344425H1), SEQ ID NO: 3040 (700347072H1), SEQ ID NO: 2411 (700346060H1), SEQ ID NO: 2477 (700346167H1), SEQ ID NO: 446 (700342755H1), SEQ ID NO: 3026 (700347050H1), SEQ ID NO: 3554 (700380452H1), SEQ ID NO: 3465 (700380146H1), SEQ ID NO: 143 (700342228H1), SEQ ID NO: 867 (700343428H1), SEQ ID NO: 1139 (700343832H1), SEQ ID NO: 3067 (700347126H1), SEQ ID NO: 799 (700343333H1), SEQ ID NO: 2059 (700345469H1), SEQ ID NO: 2120 (700345571H1), SEQ ID NO: 2179 (700345671H1), SEQ ID NO: 2707 (700346525H1), SEQ ID NO: 967 (700343572H1), SEQ ID NO: 1356 (700344174H1), SEQ ID NO: 2896 (700346848H1), SEQ ID NO: 304 (700342523H1), SEQ ID NO: 1063 (700343723H1), SEQ ID NO: 560 (700342959H1), SEQ ID NO: 2625 (700346389H1), SEQ ID NO: 2501 (700346210H1), SEQ ID NO: 319 (700342551H1), SEQ ID NO: 3173 (700347308H1), SEQ ID NO: 2313 (700345910H1), SEQ ID NO: 2022 (700345403H1), SEQ ID NO: 1076 (700343742H1), SEQ ID NO: 3025 (700347049H1), SEQ ID NO: 1719 (700344849H1), SEQ ID NO: 3342 (700379862H1), SEQ ID NO: 643 (700343103H1), SEQ ID NO: 3649 (700380682H1), SEQ ID NO: 3613 (700380589H1), SEQ ID NO: 807 (700343342H1), SEQ ID NO: 1760 (700344920H1), SEQ ID NO: 2558 (700346288H1), SEQ ID NO: 492 (700342832H1), SEQ ID NO: 504 (700342846H1), SEQ ID NO: 172 (700342278H1), SEQ ID NO: 1738 (700344878H1), SEQ ID NO: 2014 (700345384H1), SEQ ID NO: 390 (700342666H1), SEQ ID NO: 401 (700342682H1), SEQ ID NO: 3512 (700380263H1), SEQ ID NO: 3441 (700380096H1), SEQ ID NO: 2370 (700346008H1), SEQ ID NO: 1323 (700344124H1), SEQ ID NO: 634 (700343087H1), SEQ ID NO: 1986 (700345330H1), SEQ ID NO: 238 (700342403H1), SEQ ID NO: 987 (700343603H1), SEQ ID NO: 273 (700342463H1), SEQ ID NO: 208 (700342340H1), SEQ ID NO: 2674 (700346475H1), SEQ ID NO: 3119 (700347222H1), SEQ ID NO: 2203 (700345716H1), SEQ ID NO: 2186 (700345686H1), SEQ ID NO: 1307 (700344096H1), SEQ ID NO: 1372 (700344196H1), SEQ ID NO: 3053 (700347093H1), SEQ ID NO: 2507 (700346218H1), SEQ ID NO: 1049 (700343703H1), SEQ ID NO: 391 (700342667H1), SEQ ID NO: 3092 (700347176H1), SEQ ID NO: 3389 (700379975H1), SEQ ID NO: 326 (700342560H1), SEQ ID NO: 3605 (700380575H1), SEQ ID NO: 2946 (700346918H1), SEQ ID NO: 907 (700343487H1), SEQ ID NO: 3366 (700379925H1), SEQ ID NO: 1237 (700343991H1), SEQ ID NO: 3610 (700380584H1), SEQ ID NO: 2464 (700346146H1), SEQ ID NO: 3650 (700380683H1), SEQ ID NO: 2024 (700345405H1), SEQ ID NO: 1145 (700343841H1), SEQ ID NO: 1590 (700344655H1), SEQ ID NO: 2219 (700345747H1), SEQ ID NO: 224 (700342363H1), SEQ ID NO: 1108 (700343789H1), SEQ ID NO: 344 (700342589H1), SEQ ID NO: 2995 (700346996H1), SEQ ID NO: 3282 (700379719H1), SEQ ID NO: 1826 (700345020H1), SEQ ID NO: 1443 (700344334H1), SEQ ID NO: 3021 (700347040H1), SEQ ID NO: 558 (700342952H1), SEQ ID NO: 2292 (700345873H1), SEQ ID NO: 2030 (700345412H1), SEQ ID NO: 1005 (700343632H1), SEQ ID NO: 254 (700342432H1), SEQ ID NO: 1506 (700344454H1), SEQ ID NO: 1146 (700343842H1), SEQ ID NO: 1756 (700344911H1), SEQ ID NO: 2471 (700346157H1), SEQ ID NO: 1170 (700343875H1), SEQ ID NO: 1765 (700344928H1), SEQ ID NO: 1585 (700344649H1), SEQ ID NO: 495 (700342835H1), SEQ ID NO: 392 (700342670H1), SEQ ID NO: 5179 (700355947H1), SEQ ID NO: 4225 (700353526H1), SEQ ID NO: 5639 (700382063H1), SEQ ID NO: 3797 (700352605H1), SEQ ID NO: 4188 (700353271H1), SEQ ID NO: 5940 (700382691H1), SEQ ID NO: 4388 (700353955H1), SEQ ID NO: 5962 (700382739H1), SEQ ID NO: 6034 (700382890H1), SEQ ID NO: 4988 (700355520H1), SEQ ID NO: 5105 (700355788H1), SEQ ID NO: 5943 (700382702H1), SEQ ID NO: 4276 (700353625H1), SEQ ID NO: 5267 (700356140H1), SEQ ID NO: 3698 (700282414H2), SEQ ID NO: 4942 (700355431H1), SEQ ID NO: 4803 (700355029H1), SEQ ID NO: 5171 (700355925H1), SEQ ID NO: 5366 (700356466H1), SEQ ID NO: 4940 (700355428H1), SEQ ID NO: 5895 (700382605H1), SEQ ID NO: 4403 (700354129H1), SEQ ID NO: 4830 (700355088H1), SEQ ID NO: 5695 (700382183H1), SEQ ID NO: 5440 (700356708H1), SEQ ID NO: 5526 (700357108H1), SEQ ID NO: 5529 (700357122H1), SEQ ID NO: 5371 (700356474H1), SEQ ID NO: 4029 (700353005H1), SEQ ID NO: 5099 (700355779H1), SEQ ID NO: 4100 (700353128H1), SEQ ID NO: 3790 (700282575H2), SEQ ID NO: 3835 (700352675H1), SEQ ID NO: 3943 (700352843H1), SEQ ID NO: 3856 (700352709H1), SEQ ID NO: 3874 (700352735H1), SEQ ID NO: 4959 (700355462H1), SEQ ID NO: 5010 (700355558H1), SEQ ID NO: 4336 (700353828H1), SEQ ID NO: 5420 (700356627H1), SEQ ID NO: 5751 (700382275H1), SEQ ID NO: 5301 (700356219H1), SEQ ID NO: 6184 (700383158H1), SEQ ID NO: 5003 (700355547H1), SEQ ID NO: 4800 (700355026H1), SEQ ID NO: 4610 (700354647H1), SEQ ID NO: 4789 (700355009H1), SEQ ID NO: 4466 (700354337H1), SEQ ID NO: 5278 (700356158H1), SEQ ID NO: 4742 (700354908H1), SEQ ID NO: 5260 (700356129H1), SEQ ID NO: 5661 (700382106H1), SEQ ID NO: 3806 (700352616H1), SEQ ID NO: 4555 (700354518H1), SEQ ID NO: 5232 (700356066H1), SEQ ID NO: 5216 (700356023H1), SEQ ID NO: 6002 (700382827H1), SEQ ID NO: 4217 (700353446H1), SEQ ID NO: 4793 (700355016H1), SEQ ID NO: 4204 (700353409H1), SEQ ID NO: 6178 (700383149H1), SEQ ID NO: 6083 (700382979H1), SEQ ID NO: 4876 (700355249H1), SEQ ID NO: 4031 (700353007H1), SEQ ID NO: 6098 (700383013H1), SEQ ID NO: 6102 (700383021H1), SEQ ID NO: 4421 (700354239H1), SEQ ID NO: 5946 (700382708H1), SEQ ID NO: 5214 (700356020H1), SEQ ID NO: 5703 (700382195H1), SEQ ID NO: 3750 (700282503H2), SEQ ID NO: 4212 (700353432H1), SEQ ID NO: 6090 (700382995H1), SEQ ID NO: 4713 (700354850H1), SEQ ID NO: 4197 (700353284H1), SEQ ID NO: 5692 (700382179H1), SEQ ID NO: 4727 (700354875H1), SEQ ID NO: 5924 (700382668H1), SEQ ID NO: 4052 (700353052H1), SEQ ID NO: 4111 (700353142H1), SEQ ID NO: 4043 (700353028H1), SEQ ID NO: 5723 (700382229H1), SEQ ID NO: 5560 (700357402H1), SEQ ID NO: 5603 (700357489H1), SEQ ID NO: 5700 (70038219151), SEQ ID NO: 5875 (700382543H1), SEQ ID NO: 3811 (700352625H1), SEQ ID NO: 6081 (700382975H1), SEQ ID NO: 5186 (700355963H1), SEQ ID NO: 5813 (700382392H1), SEQ ID NO: 5155 (700355889H1), SEQ ID NO: 4632 (700354689H1), SEQ ID NO: 5674 (700382139H1), SEQ ID NO: 4279 (700353629H1), SEQ ID NO: 4625 (700354676H1), SEQ ID NO: 5586 (700357445H1), SEQ ID NO: 4054 (700353057H1), SEQ ID NO: 5724 (700382230H1), SEQ ID NO: 5699 (700382190H1), SEQ ID NO: 6181 (700383154H1), SEQ ID NO: 3982 (700352912H1), SEQ ID NO: 4654 (700354742H1), SEQ ID NO: 4737 (700354895H1), SEQ ID NO: 4321 (700353779H1), SEQ ID NO: 4934 (700355415H1), SEQ ID NO: 5543 (700357160H1), SEQ ID NO: 4933 (700355414H1), SEQ ID NO: 3713 (700282435H1), SEQ ID NO: 3787 (700282569H2), SEQ ID NO: 3789 (700282571H2), SEQ ID NO: 4814 (700355053H1), SEQ ID NO: 5555 (700357189H1), SEQ ID NO: 3876 (700352737H1), SEQ ID NO: 4208 (700353424H1), SEQ ID NO: 3827 (700352654H1), SEQ ID NO: 3863 (700352719H1), SEQ ID NO: 6130 (700383062H1), SEQ ID NO: 4041 (700353023H1), SEQ ID NO: 5101 (700355781H1), SEQ ID NO: 5792 (700382353H1), SEQ ID NO: 4983 (700355513H1), SEQ ID NO: 4089 (700353114H1), SEQ ID NO: 4348 (700353853H1), SEQ ID NO: 6182 (700383155H1), SEQ ID NO: 5716 (700382218H1), SEQ ID NO: 5102 (700355783H1), SEQ ID NO: 5859 (700382507H1), SEQ ID NO: 5442 (700356725H1), SEQ ID NO: 5998 (700382817H1), SEQ ID NO: 4037 (700353014H1), SEQ ID NO: 4404 (700354142H1), SEQ ID NO: 5734 (700382245H1), SEQ ID NO: 5392 (700356534H1), SEQ ID NO: 5675 (700382140H1), SEQ ID NO: 5746 (700382267H1), SEQ ID NO: 4102 (700353130H1), SEQ ID NO: 4536 (700354478H1), SEQ ID NO: 4219 (700353509H1), SEQ ID NO: 5151 (700355885H1), SEQ ID NO: 4268 (700353612H1), SEQ ID NO: 3774 (700282549H2), SEQ ID NO: 3772 (700282547H2), SEQ ID NO: 5283 (700356171H1), SEQ ID NO: 5647 (700382075H1), SEQ ID NO: 5778 (700382330H1), SEQ ID NO: 5014 (700355577H1), SEQ ID NO: 5825 (700382426H1), SEQ ID NO: 5866 (700382525H1), SEQ ID NO: 4556 (700354519H1), SEQ ID NO: 3821 (700352645H1), SEQ ID NO: 4762 (700354942H1), SEQ ID NO: 4981 (700355507H1), SEQ ID NO: 5540 (700357155H1), SEQ ID NO: 5476 (700356835H1), SEQ ID NO: 5650 (700382078H1), SEQ ID NO: 5750 (700382273H1), SEQ ID NO: 5100 (700355780H1), SEQ ID NO: 4553 (700354516H1), SEQ ID NO: 4923 (700355345H1), SEQ ID NO: 4922 (700355344H1), SEQ ID NO: 3773 (700282548H2), SEQ ID NO: 3715 (700282438H2), SEQ ID NO: 5022 (700355594H1), SEQ ID NO: 5516 (700356974H1), SEQ ID NO: 4451 (700354305H1), SEQ ID NO: 4938 (700355422H1), SEQ ID NO: 5436 (700356695H1), SEQ ID NO: 5742 (700382260H1), SEQ ID NO: 5242 (700356081H1), SEQ ID NO: 4766 (700354954H1), SEQ ID NO: 3792 (700282584H2), SEQ ID NO: 3795 (700282592H2), SEQ ID NO: 5569 (700357415H1), SEQ ID NO: 5142 (700355871H1), SEQ ID NO: 4305 (700353713H1), SEQ ID NO: 4559 (700354534H1), SEQ ID NO: 5188 (700355966H1), SEQ ID NO: 5088 (700355766H1), SEQ ID NO: 5286 (700356177H1), SEQ ID NO: 5294 (700356193H1), SEQ ID NO: 3995 (700352935H1), SEQ ID NO: 4615 (700354658H1), SEQ ID NO: 5453 (700356767H1), SEQ ID NO: 4667 (700354769H1), SEQ ID NO: 5832 (700382439H1), SEQ ID NO: 5839 (700382450H1), SEQ ID NO: 6101 (700383019H1), SEQ ID NO: 6097 (700383011H1), SEQ ID NO: 4951 (700355447H1), SEQ ID NO: 4423 (700354247H1), SEQ ID NO: 4672 (700354777H1), SEQ ID NO: 4682 (700354793H1), SEQ ID NO: 5395 (700356541H1), SEQ ID NO: 5311 (700356241H1), SEQ ID NO: 4522 (700354446H1), SEQ ID NO: 4460 (700354326H1), SEQ ID NO: 5493 (700356901H1), SEQ ID NO: 5507 (700356933H1), SEQ ID NO: 4512 (700354425H1), SEQ ID NO: 5217 (700356025H1), SEQ ID NO: 4159 (700353215H1), SEQ ID NO: 5480 (700356847H1), SEQ ID NO: 6123 (700383051H1), SEQ ID NO: 4452 (700354310H1), SEQ ID NO: 4499 (700354409H1), SEQ ID NO: 4808 (700355040H1), SEQ ID NO: 5800 (700382365H1), SEQ ID NO: 5710 (700382210H1), SEQ ID NO: 6030 (700382882H1), SEQ ID NO: 5461 (700356796H1), SEQ ID NO: 5548 (700357173H1), SEQ ID NO: 4790 (700355011H1), SEQ ID NO: 5201 (700355989H1), SEQ ID NO: 4286 (700353653H1), SEQ ID NO: 6103 (700383023H1), SEQ ID NO: 6099 (700383015H1), SEQ ID NO: 5317 (700356252H1), SEQ ID NO: 5399 (700356551H1), SEQ ID NO: 5667 (700382123H1), SEQ ID NO: 6176 (700383147H1), SEQ ID NO: 5084 (700355758H1), SEQ ID NO: 4566 (700354558H1), SEQ ID NO: 4845 (700355173H1), SEQ ID NO: 4819 (700355067H1), SEQ ID NO: 3775 (700282550H2), SEQ ID NO: 5273 (700356152H1), SEQ ID NO: 6111 (700383037H1), SEQ ID NO: 3984 (700352916H1), SEQ ID NO: 5481 (700356848H1), SEQ ID NO: 5309 (700356236H1), SEQ ID NO: 4456 (700354317H1), SEQ ID NO: 4824 (700355077H1), SEQ ID NO: 5240 (700356076H1), SEQ ID NO: 5121 (700355829H1), SEQ ID NO: 5418 (700356624H1), SEQ ID NO: 4603 (700354637H1), SEQ ID NO: 5718 (700382220H1), SEQ ID NO: 5747 (700382268H1), SEQ ID NO: 6189 (700383166H1), SEQ ID NO: 6129 (700383061H1), SEQ ID NO: 5494 (700356903H1), SEQ ID NO: 5508 (700356935H1), SEQ ID NO: 4797 (700355022H1), SEQ ID NO: 5691 (700382176H1), SEQ ID NO: 5862 (700382513H1), SEQ ID NO: 5917 (700382654H1), SEQ ID NO: 5328 (700356273H1), SEQ ID NO: 3971 (700352891H1), SEQ ID NO: 5463 (700356802H1), SEQ ID NO: 4588 (700354606H1), SEQ ID NO: 4342 (700353839H1), SEQ ID NO: 5376 (700356483H1), SEQ ID NO: 5136 (700355856H1), SEQ ID NO: 5154 (700355888H1), SEQ ID NO: 5916 (700382649H1), SEQ ID NO: 5869 (700382537H1), SEQ ID NO: 5231 (700356062H1), SEQ ID NO: 4194 (700353279H1), SEQ ID NO: 4978 (700355496H1), SEQ ID NO: 5923 (700382665H1), SEQ ID NO: 4755 (700354930H1), SEQ ID NO: 4008 (700352961H1), SEQ ID NO: 5424 (700356644H1), SEQ ID NO: 4035 (700353012H1), SEQ ID NO: 5323 (700356265H1), SEQ ID NO: 4818 (700355065H1), SEQ ID NO: 5367 (700356468H1), SEQ ID NO: 5888 (700382579H1), SEQ ID NO: 3690 (700282401H2), SEQ ID NO: 3794 (700282591H2), SEQ ID NO: 3764 (700282522H2), SEQ ID NO: 3758 (700282514H2), SEQ ID NO: 5128 (700355842H1), SEQ ID NO: 3818 (700352636H1), SEQ ID NO: 3781 (700282561H2), SEQ ID NO: 3782 (700282562H2), SEQ ID NO: 4889 (700355286H1), SEQ ID NO: 6104 (700383026H1), SEQ ID NO: 5138 (700355861H1), SEQ ID NO: 5140 (700355866H1), SEQ ID NO: 6006 (700382834H1), SEQ ID NO: 5880 (700382554H1), SEQ ID NO: 6116 (700383043H1), SEQ ID NO: 5774 (700382324H1), SEQ ID NO: 4024 (700352993H1), SEQ ID NO: 4820 (700355070H1), SEQ ID NO: 5438 (700356702H1), SEQ ID NO: 4150 (700353201H1), SEQ ID NO: 5909 (700382636H1), SEQ ID NO: 5551 (700357183H1), SEQ ID NO: 5055 (700355708H1), SEQ ID NO: 4551 (700354508H1), SEQ ID NO: 3975 (700352903H1), SEQ ID NO: 5793 (700382354H1), SEQ ID NO: 5823 (700382424H1), SEQ ID NO: 3915 (700352802H1), SEQ ID NO: 5146 (700355877H1), SEQ ID NO: 5879 (700382550H1), SEQ ID NO: 5627 (700382041H1), SEQ ID NO: 4211 (700353431H1), SEQ ID NO: 5894 (700382604H1), SEQ ID NO: 5806 (700382377H1), SEQ ID NO: 5558 (700357194H1), SEQ ID NO: 5396 (700356542H1), SEQ ID NO: 3892 (700352760H1), SEQ ID NO: 5211 (700356015H1), SEQ ID NO: 4011 (700352967H1), SEQ ID NO: 5282 (700356167H1), SEQ ID NO: 5978 (700382773H1), SEQ ID NO: 4898 (700355307H1), SEQ ID NO: 5522 (700356984H1), SEQ ID NO: 5226 (700356047H1), SEQ ID NO: 3777 (700282554H2), SEQ ID NO: 3776 (700282553H2), SEQ ID NO: 5817 (700382401H1), SEQ ID NO: 5858 (700382494H1), SEQ ID NO: 4627 (700354678H1), SEQ ID NO: 5411 (700356591H1), SEQ ID NO: 4257 (700353590H1), SEQ ID NO: 4833 (700355093H1), SEQ ID NO: 4148 (700353195H1), SEQ ID NO: 5210 (700356014H1), SEQ ID NO: 4422 (700354244H1), SEQ ID NO: 4949 (700355444H1), SEQ ID NO: 4481 (700354371H1), SEQ ID NO: 6095 (700383009H1), SEQ ID NO: 4670 (700354774H1), SEQ ID NO: 4680 (700354790H1).
- 4. A nucleic acid sequence complementary to at least a fragment of at least one corn tassel-derived polynucleotide of claim 1.
- 5. A method for detecting a polynucleotide in a biological sample, the method comprising the steps of:
a) providing a biological sample containing nucleic acids, b) hybridizing a polynucleotide of claim 4 to nucleic acids in the biological sample, thereby forming a hybridization complex; and c) detecting the hybridization complex, wherein the presence of the hybridization complex correlates with the presence of the polynucleotide in the sample.
- 6. The method of claim 5 wherein the nucleic acids of the biological sample are amplified prior to hybridization.
- 7. A method for using oligomers and amplification to recover a regulatory element from a DNA library, the method comprising the steps of:
a) designing oligomers to at least one of the sequences of Claim b) combining the oligomers with a DNA library under appropriate conditions to amplify the sequence of step a); c) comparing the nucleic acid sequence of step a) with the amplified sequence to identify overlapping areas; d) identifying additional sequence beyond the overlapping areas, and e) repeating steps a) through d) until the regulatory element is recovered.
- 8. A tassel-specific regulatory element which regulates the expression of at least one of the sequences of claim 1.
- 9. An expression vector containing the regulatory element of claim 8.
- 10. A plant containing the expression vector of claim 9.
- 11. An expression vector containing the corn tassel-derived polynucleotide of claim 1.
- 12. A host cell containing the expression vector of claim 11.
- 13. A method for producing a corn tassel-derived polypeptide, said method comprising the steps of:
a) culturing the host cells of claim 12 under conditions suitable for the expression of the corn tassel-derived polypeptide; and b) recovering the corn tassel-derived polypeptide from the cell culture.
- 14. A purified corn tassel-derived polypeptide (CDP) encoded by at least one of the corn tassel-derived polynucleotides of claim 1.
- 15. An anti-CDP antibody specifically binding the purified corn tassel-derived polypeptide of claim 14.
- 16. A method of identifying a test compound which specifically binds to the polypeptide of claim 14, the method comprising the steps of:
a) providing a test compound; b) combining the polypeptide with the test compound for a sufficient time and under suitable conditions for binding; and c) detecting binding of the polypeptide to the test compound, thereby identifying the compound which specifically binds the polypeptide.
- 17. A method of screening a plurality of molecules for specific binding to the polynucleotide of claim 1, the method comprising the steps of:
a) providing the plurality of molecules; b) combining the polynucleotide with each of the plurality of molecules for a time sufficient to allow binding under suitable conditions; and c) detecting binding of the polynucleotide to each of the plurality of molecules, thereby identifying the molecules which specifically bind the polynucleotide.
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Application No. 60/082,567, our Docket No. PL-0009 P, filed on Apr. 21, 1998.
[0002] A portion of the disclosure of this patent document contains material which is subject to copyright protection. The copyright owner has no objection to the facsimile reproduction by anyone of the patent document or the patent disclosure, as it appears in the Patent and Trademark Office patent file or records, but otherwise reserves all copyright rights whatsoever.
Provisional Applications (1)
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Number |
Date |
Country |
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60082567 |
Apr 1998 |
US |