Claims
- 1. An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
(a) a nucleotide sequence encoding a polypeptide comprising amino acids from about −20 to about 129 in SEQ ID NO:2; (b) a nucleotide sequence encoding a polypeptide comprising amino acids from about −19 to about 129 in SEQ ID NO:2; (c) a nucleotide sequence encoding a polypeptide comprising amino acids from about 1 to about 129 in SEQ ID NO:2; (d) a nucleotide sequence encoding a polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519; (e) a nucleotide sequence encoding the mature chemokine β-15 polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519; and (f) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d), or (e).
- 2. The nucleic acid molecule of claim 1 wherein said polynucleotide has the complete nucleotide sequence in SEQ ID NO:1.
- 3. The nucleic acid molecule of claim 1 wherein said polynucleotide has the nucleotide sequence in SEQ ID NO:1 encoding the chemokine β-15 polypeptide having the complete amino acid sequence in SEQ ID NO:2.
- 4. The nucleic acid molecule of claim 1 wherein said polynucleotide has the nucleotide sequence in SEQ ID NO:1 encoding the mature chemokine β-15 polypeptide having the amino acid sequence in SEQ ID NO:2.
- 5. The nucleic acid molecule of claim 1 wherein said polynucleotide has the complete nucleotide sequence of the cDNA clone contained in ATCC Deposit No. 97519.
- 6. The nucleic acid molecule of claim 1 wherein said polynucleotide has the nucleotide sequence encoding the chemokine β-15 polypeptide having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519.
- 7. The nucleic acid molecule of claim 1 wherein said polynucleotide has the nucleotide sequence encoding the mature chemokine β-15 polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519.
- 8. An isolated nucleic acid molecule comprising a polynucleotide which hybridizes under stringent hybridization conditions to a polynucleotide having a nucleotide sequence identical to a nucleotide sequence in (a), (b), (c), (d), (e), or (f) of claim 1 wherein said polynucleotide which hybridizes does not hybridize under stringent hybridization conditions to a polynucleotide having a nucleotide sequence consisting of only A residues or of only T residues.
- 9. An isolated nucleic acid molecule comprising a polynucleotide which encodes the amino acid sequence of an epitope-bearing portion of a chemokine β-15 polypeptide having an amino acid sequence in (a), (b), (c), (d), or (e) of claim 1.
- 10. A method for making a recombinant vector comprising inserting an isolated nucleic acid molecule of claim 1 into a vector.
- 11. A recombinant vector produced by the method of claim 10.
- 12. A method of making a recombinant host cell comprising introducing the recombinant vector of claim 11 into a host cell.
- 13. A recombinant host cell produced by the method of claim 12.
- 14. A recombinant method for producing a chemokine β-15 polypeptide, comprising culturing the recombinant host cell of claim 13 under conditions such that said polypeptide is expressed and recovering said polypeptide.
- 15. An isolated chemokine β-15 polypeptide having an amino acid sequence at least 95% identical to a sequence selected from the group consisting of:
(a) amino acids from about −20 to about 129 in SEQ ID NO:2; (b) amino acids from about −19 to about 129 in SEQ ID NO:2; (c) amino acids from about 1 to about 129 in SEQ ID NO:2; (d) the amino acid sequence of the chemokine β-15 polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519; (e) the amino acid sequence of the mature chemokine β-15 polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519; and (f) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c), (d), or (e).
- 16. An isolated antibody that binds specifically to a chemokine β-15 polypeptide of claim 15.
- 17. An isolated nucleic acid molecule comprising a polynucleotide encoding a chemokine β-15 polypeptide wherein, except for at least one conservative amino acid substitution, said polypeptide has a sequence selected from the group consisting of:
(a) a nucleotide sequence encoding a polypeptide comprising amino acids from about −20 to about 129 in SEQ ID NO:2; (b) a nucleotide sequence encoding a polypeptide comprising amino acids from about −19 to about 129 in SEQ ID NO:2; (c) a nucleotide sequence encoding a polypeptide comprising amino acids from about 1 to about 129 in SEQ ID NO:2; (d) a nucleotide sequence encoding a polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519; (e) a nucleotide sequence encoding the mature chemokine β-15 polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519; and (f) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d), or (e).
- 18. An isolated chemokine β-15 polypeptide wherein, except for at least one conservative amino acid substitution, said polypeptide has a sequence selected from the group consisting of:
(a) amino acids from about −20 to about 129 in SEQ ID NO:2; (b) amino acids from about −19 to about 129 in SEQ ID NO:2; (c) amino acids from about 1 to about 129 in SEQ ID NO:2; (d) the amino acid sequence of the chemokine β-15 polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519; (e) the amino acid sequence of the mature chemokine β-15 polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97519; and (f) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c), (d), or (e).
- 19. A method for treatment of an individual in need of an increased level of chemokine β-15 activity comprising administering to said individual a composition comprising an isolated polypeptide of claim 15.
- 20. A method useful during the diagnosis of a disorder of the thymus in an individual comprising:
(a) measuring chemokine β-15 gene expression level in cells or body fluid of said individual; (b) comparing the chemokine β-15 gene expression level of said individual with a standard chemokine β-15 gene expression level, whereby an increase or decrease in the chemokine β-15 gene expression level of said individual compared to said standard expression level is indicative of a thymus disorder.
Parent Case Info
[0001] This application claims the benefit of the priority date of provisional application 60/019,837 filed on Jun. 17, 1996, which is herein incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60019837 |
Jun 1996 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
08874460 |
Jun 1997 |
US |
Child |
09272162 |
Mar 1999 |
US |