Claims
- 1. A composition for inducing an immunogenic response in an animal, comprising:
- an antigen and a polyphosphazene polyelectrolyte adjuvant wherein at least a portion of said polyphosphazene polyelectrolyte has the formula: ##STR6## wherein each A is independently selected from X or Y in which (1) X is ##STR7## in which R.sub.1 is --N(R.sub.4)--, --O-- or --OR.sub.5 -- where R.sub.4 is hydrogen or a C.sub.1 -C.sub.4 alkyl(preferably hydrogen) and R.sub.5 is a C.sub.1 -C.sub.3 alkylene(preferably methylene) moiety, R.sub.2 is at least one carboxylic acid moiety which can be attached at any ring position directly or through an alkylene, preferably a methylene and R.sub.3 is hydrogen or another substituent moiety such as an alkoxy, for example a methoxy moiety;
- (2) Y is selected from the group consisting of
- (a) (OCH.sub.2 CH.sub.2).sub.n OR.sub.6 in which n is 1 to 120 and R.sub.6 is a C.sub.1 to C.sub.10 hydrocarbon; and
- (b) (B)OR.sub.7 in which B is a polyoxypropylene/polyoxyethylene block copolymer and R.sub.7 is a C.sub.1 to C.sub.10 hydrocarbon, an aliphatic saturated or unsaturated hydrocarbon or an aromatic hydrocarbon, including alkyl substituted aromatic hydrocarbons;
- (3) X and Y are in a range of ratios of 1:40 to 40:1; and
- (4) m is 3 to 100,000.
- 2. The composition of claim 1 wherein the polymer contains at least 10 percent or more of repeating units that are not susceptible to hydrolysis under the conditions of use.
- 3. The composition of claim 1 wherein the polyphosphazene polyelectrolyte is cross-linked with a multivalent cation.
- 4. The composition of claim 3 wherein the multivalent cation is selected from the group consisting of calcium, copper, aluminum, magnesium, strontium, barium, tin, zinc, iron, poly(amino acid), poly(ethyleneimine), poly(vinylamine) and polysaccharides.
- 5. The composition of claim 1 wherein the antigen is selected from the group consisting of protein, peptide, polysaccharide, glycoprotein, glycolipid, nucleic acid, or a combination thereof derived from a cell, bacteria, virus particle or a portion of any of them.
- 6. The composition of claim 1 wherein the polyphosphazene polyelectrolyte is covalently conjugated with an antigen.
- 7. The composition of claim 1 wherein X is selected from the group consisting of: ##STR8##
- 8. A method of inducing an immune response in an animal comprising the steps of administering to the animal an antigen and a polyphosphazene polyelectrolyte immunoadjuvant, wherein at least a portion of said polyphosphazene polyelectrolyte has the formula: wherein each A is independently selected from X or Y in which
- (1) X is ##STR9## in which R.sub.1 is --N(R.sub.4)--, --O-- or --OR.sub.5 -- where R.sub.4 is hydrogen or a C.sub.4 -C.sub.4 alkyl(preferably hydrogen) and R.sub.5 C.sub.1 -C.sub.3 alkylene (preferably methylene) moiety, R.sub.2 is at least one carboxylic acid moiety which can be attached at any ring position directly or through an alkylene, preferably a methylene and R.sub.3 is hydrogen or another substituent moiety such as an alkoxy, for example a methoxy moiety;
- (2) Y is selected from the group consisting of
- (a) (OCH.sub.2 CH.sub.2).sub.n OR.sub.6 in which n is 1 to 120 and R.sub.6 is a C.sub.1 to C.sub.10 hydrocarbon; and
- (b) (B)OR.sub.7 in which B is a polyoxypropylene/polyoxyethylene block copolymer and R.sub.7 is a C.sub.1 to C.sub.10 hydrocarbon, an aliphatic saturated or unsaturated hydrocarbon or an aromatic hydrocarbon, including alkyl substituted aromatic hydrocarbons;
- (3) X and Y are in a range of ratios of 1:40 to 40:1; and
- (4) m is 3 to 100,000.
- 9. The method of claim 8 wherein the antigen is conjugated with the polyphosphazene polyelectrolyte.
- 10. The method of claim 8 wherein the antigen and polyphosphazene are administered separately to proximate sites.
- 11. The method of claim 8 wherein the antigen and polyphosphazene are first combined and the combination is administered to the animal.
- 12. The method of claim 8 wherein the method of administration is parenteral.
- 13. The method of claim 8 wherein the method of administration is oral.
- 14. A polyphosphazene polyelectrolyte adjuvant wherein at least a portion of said polyphosphazene polyelectrolyte has the formula: ##STR10## wherein each A is independently selected from X or Y in which (1) X is ##STR11## in which R.sub.1 is --N(R.sub.4)--, --O-- or --OR.sub.5 -- where R.sub.4 is hydrogen or a C.sub.1 -C.sub.4 alkyl(preferably hydrogen) and R.sub.5 is a C.sub.1 -C.sub.3 alkylene(preferably methylene) moiety, R.sub.2 is at least one carboxylic acid moiety which can be attached at any ring position directly or through an alkylene, preferably a methylene and R.sub.3 is hydrogen or another substituent moiety such as an alkoxy, for example a methoxy moiety;
- (2) Y is selected from the group consisting of
- (a) (OCH.sub.2 CH.sub.2).sub.n OR.sub.6 in which n is 1 to 120 and R.sub.6 is a C.sub.1 to C.sub.10 hydrocarbon; and
- (b) (B)OR.sub.7 in which B is a polyoxypropylene/polyoxyethylene block copolymer and R.sub.7 is a C.sub.1 to C.sub.10 hydrocarbon, an aliphatic saturated or unsaturated hydrocarbon or an aromatic hydrocarbon, including alkyl substituted aromatic hydrocarbons;
- (3) X and Y are in a range of ratios of 1:40 to 40:1; and
- (4) m is 3 to 100,000.
- 15. The polyphosphazene polyelectrolyte of claim 14 wherein the polymer contains at least 10 percent or more of repeating units that are not susceptible to hydrolysis under the conditions of use.
- 16. The polyphosphazene polyelectrolyte of claim 14 wherein the polyphosphazene polyelectrolyte is cross-linked with a multivalent cation.
- 17. The polyphosphazene polyelectrolyte of claim 16 wherein the multivalent cation is selected from the group consisting of calcium, copper, aluminum, magnesium, strontium, barium, tin, zinc, iron, poly(amino acid), poly(ethyleneimine), poly(vinylamine) and polysaccharides.
- 18. The polyphosphazene polyelectrolyte of claim 14 wherein the antigen is selected from the group consisting of protein, peptide, polysaccharide, glycoprotein, glycolipid, nucleic acid, or a combination thereof derived from a cell, bacteria, virus particle or a portion of any of them.
- 19. The polyphosphazene polyelectrolyte of claim 14 wherein the polyphosphazene polyelectrolyte is covalently conjugated with an antigen.
- 20. The polyphosphazene polyelectrolyte of claim 14 wherein X is selected from the group consisting of: ##STR12##
- 21. A polyphosphazene polyelectrolyte adjuvant wherein at least a portion of said polyphosphazene polyelectrolyte has the formula: wherein each B is
- Ortho-O--C.sub.6 H.sub.4 COOH.
- 22. A composition for inducing an immunogenic response in an animal, comprising:
- an antigen and a polyphosphazene polyelectrolyte adjuvant wherein at least a portion of said polyphosphazene polyelectrolyte has the formula: ##STR13## wherein m is 3 to 100,000 and each B is
- Ortho-O--C.sub.6 H.sub.4 COOH.
Parent Case Info
This is a Divisional of application Ser. No. 08/478,522 filed Jun. 7, 1995.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
5284934 |
Allen, Jr. |
Feb 1994 |
|
5494673 |
Adrianov et al. |
Feb 1996 |
|
5855895 |
Andrianov et al. |
Jan 1999 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO 9409822 |
May 1994 |
WOX |
Non-Patent Literature Citations (2)
Entry |
Cohen, et al., J. Am. Chem. Soc., vol. 112, pp. 7832-7833 (1990). |
Andrianov, et al., J. Controlled Release, vol. 27, pp. 69-77 (1993). |
Divisions (1)
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Number |
Date |
Country |
Parent |
478552 |
Jun 1995 |
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