Claims
- 1. The process for preparing Heteropolysaccharide 10, the heteropolysaccharide containing about 3% protein and about 97% carbohydrate, the carbohydrate portion of which contains about 19% of a uronic acid, about 39% glucose, about 29% galactose and about 13% fucose, said heteropolysaccharide being compatible with Methylene Blue chloride dye, that comprises cultivating under submerged aerobic conditions at a temperature of about 28.degree. to about 35.degree. C. a heteropolysaccharide producing strain of Klebsiella pneumoniae or a heteropolysaccharide producing mutant thereof in an aqueous nutrient medium containing as a source of carbon hydrolyzed starch having a dextrose equivalent of about 20-35, a source of nitrogen, a source of magnesium and a source of phosphorus, until substantial viscosity has been imparted to said medium, and recovering the heteropolysaccharide.
- 2. The process of claim 1 wherein the fermentation is carried out for from about 45-60 hours.
- 3. The process of claim 2 wherein the carbon source is a corn starch hydrolyzate.
- 4. The process of claim 1 wherein the recovery of heteropolysaccharide is effected by precipitation with lower alkanol.
- 5. The process of claim 4 wherein the lower alkanol is isopropanol.
- 6. The process of claim 1 wherein the strain is ATCC 21711.
- 7. An aqueous nutrient medium containing a Heteropolysaccharide 10 producing strain of Klebsiella pneumoniae or a heteropolysaccharide 10 producing mutant thereof and as a source of carbon hydrolyzed starch having a dextrose equivalent of about 20-35, a source of nitrogen, a source of magnesium and a source of phosphorus.
- 8. An aqueous fermentation medium containing a strain of Heteropolysaccharide 10-producing Klebsiella pneumoniae or a heteropolysaccharide 10-producing mutant thereof, as a source of carbon hydrolyzed starch having a dextrose equivalent of about 20-35, a source of nitrogen, a source of magnesium and a source of phosphorus, and heteropolysaccharide 10 produced by the strain or the mutant.
RELATED APPLICATIONS
This application is a continuation-in-part of application Ser. No. 806,801 filed June 15, 1977, now abandoned, which is a continuation of application Ser. No. 725,595 filed Sept. 22, 1976, now abandoned which, in turn, is a continuation of application Ser. No. 616,739 filed Sept. 25, 1975, now abandoned, which is a division of application Ser. No. 373,724 filed June 26, 1973, now U.S. Pat. No. 3,933,788 which, in turn, is a continuation-in-part of application Ser. No. 197,941, filed Nov. 11, 1971, now abandoned.
US Referenced Citations (4)
Foreign Referenced Citations (1)
Number |
Date |
Country |
1411014 |
Oct 1975 |
GBX |
Non-Patent Literature Citations (3)
Entry |
Buchanan et al., Bergey's Manual of Determinative Bacteriology, 8th Ed. Williams & Wilkins Co., Baltimore (1974) pp. 321-324. |
Duguid et al., "The Influence of Cultural Conditions on Polysaccharide Production by Aerobacter aerogenes", J. Gen. Micro., vol. 9 No. 2, (1953), pp. 174-189. |
Smith et al., "Volutin Production in Aerobacter aerogenes Due to Nutrient Imbalance", J. Bact., vol. 68, No. 4 (1954) pp. 450-463. |
Divisions (1)
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Date |
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Parent |
373724 |
Jun 1973 |
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Continuations (2)
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Date |
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Parent |
725595 |
Sep 1976 |
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Parent |
616739 |
Sep 1975 |
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Continuation in Parts (2)
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Date |
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806801 |
Jun 1977 |
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Parent |
197941 |
Nov 1971 |
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