Claims
- 1. A water-soluble, heat resistant conjugate of a polysaccharide, obtained from a pathogenic bacterial organism, covalently bound to a T-cell dependent antigen
- capable of producing a T-cell dependent antibody response to polysaccharides from the said organism
- of molecular size above 140,000 and below 4,500,000 daltons as referenced to dextran standard by gel permeation chromatography, and
- having a polysaccharide/protein ratio between 0.25 and 2, prepared by mixing a T-cell dependent antigen with a spacer compound of the formula X-B-X, wherein B is a C.sup.4-8 alkylene in which a --CH.sub.2 -- linkage can optionally be replaced by an --O-- linkage and wherein X is a nucleophilic group capable of reacting with said antigen,
- mixing the resulting derivatized antigen with an electrophilically activated polysaccharide obtained from the said bacterial organism, which polysaccharide had previously been sized by heating until more than 60% of the polysaccharide was adjusted to a molecular size between 200,000 and 2,000,000 dalton as referenced to dextran standard by gel permeation chromatograpy.
- 2. A conjugate of claim 1 where said organism is a strain of meningococcus.
- 3. A conjugate of claim 1 where said organism is E. coli.
- 4. A conjugate of claim 2 where said organism is a strain of pneumococcus.
- 5. A conjugate of claim 1 wherein said organism is a strain of streptococcus.
- 6. A conjugate of claim 1 wherein X-B-X is a dicarboxylic acid hydrazide.
- 7. A conjugate of claim 6 wherein said dicarboxylic acid is adipic acid.
- 8. A conjugate of claim 1 wherein said T-cell dependent antigen is diphtheria toxoid.
- 9. A conjugate of claim 1 wherein said T-cell dependent antigen is tetanus toxoid.
- 10. A conjugate of claim 1 wherein said electrophilically activated polysaccharide is activated by use of a cyanogen halide as activator.
- 11. A conjugate of claim 10 wherein said cyanogen halide is the bromide.
- 12. A conjugate of claim 1 wherein said electrophilically activated polysaccharide is activated by use of an alkali metal borohydride as activator.
- 13. A conjugate of claim 12 wherein said alkali metal borohydride is sodium borohydride.
Parent Case Info
This is a continuation-in-part of Ser. No. 395,743 filed July 6, 1982, which issued as U.S. Pat. No. 4,496,538 on Jan. 29, 1985.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4356170 |
Jennings et al. |
Oct 1982 |
|
Non-Patent Literature Citations (1)
Entry |
Schneerson, R., et al., J. Exp. Med., vol. 152, pp. 361-376, 1980. |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
395743 |
Jul 1982 |
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