This application is claims the priority benefit under 35 U.S.C. §119 to German Patent Application No. 10 2013 102 440.8, filed on Mar. 12, 2013, entitled “Positioning means for a measuring cell” [Attorney Docket 25.728] the disclosure of which is incorporated herein by reference.
The present invention relates to a positioning means for a measuring cell for optical detection, such as is used in the area of HPLC (high performance liquid chromatography).
By means of such measuring cells, for example, the variation over time of the transmission of an eluting liquid is determined in order to obtain a chromatogram. The eluting liquid (below: sample) is led through the flow channel of a flow cell and irradiated with suitable light longitudinally or transversely with respect to the flow direction. The flow channel of the flow cell, through which the sample and the light are led, is usually formed as a thin tube with a diameter in the millimeter and sub-millimeter range.
DE 36 03 905 A1 discloses a through-flow particle analyzer, in which a suspension is examined by shining light in. An objective structure, spaced apart by a layer of dispersion oil, adjoins a flow cell, from which reflected light for evaluation enters the objective structure.
U.S. Pat. No. 7,184,141 B2 describes an optical flow cell, in which a liquid to be examined is conveyed and is transilluminated transversely with respect to the conveying direction, in order to be able to draw conclusions about the liquid by using the evaluation of the emerging light.
An optical measuring cell is further also disclosed by US 2011/0299067 A1.
In one embodiment of the detection, the light is radiated into the tube along the axial direction thereof via an optical conductor. According to one variant, the optical conductor must be positioned as accurately centrally as possible with respect to the tube axis and, between itself and the inner wall of the tube, form an annular gap, through which the sample flows into the tube. The exactly central positioning of the optical conductor and the resultant formation of a uniform annular gap are particularly advantageous here to optimizing the flow. The central alignment is, however, not absolutely necessary, according to the general idea of the invention; the accurate arrangement of the optical conductor in a predefined relative position and attitude in relation to the tube is important.
The optical conductor is usually introduced into the tube by means of a specific connecting piece; the sample is also fed to the tube at the same time via the connecting piece. For the accurate arrangement of the optical conductor within the tube, it is therefore necessary to align the connecting piece exactly relative to the tube. Here, the optical conductor should assume an arrangement in space of which the attitude (position) and angle of rotation (alignment) are determined by the tube, for example collinearly and/or centrally with respect thereto.
The object of the invention was therefore to offer a device for ensuring the accurate arrangement of an optical conductor within the tube. The object was further the creation of a method for ensuring the exact positioning.
In order to hold the previously described tube stably, a flange in particular is suitable, which carries the tube in a central opening, the end face of the tube preferably ending flush with the surface of the flange facing away from the tube, following a machining step. At the other end of the tube, a further corresponding flange can be provided; both flanges can also hold a protective pipe, within which the tube is arranged and located protected. Such an arrangement (with or without protective pipe) is to be designated a flow cell below. The outer face of a flange, facing away from the tube, forms one end of the flow cell. At this end, in practice the connecting piece having the optical conductor and the sample feed are arranged and connected, attention having to be paid to the aforementioned exact arrangement (alignment and positioning) of the optical conductor and of the connecting piece relative to the tube held in the flange.
This accurate alignment is made possible by a positioning means according to the invention. The positioning means comprises a base which, in a preferred embodiment, is designed to be at least partially rotationally symmetrical. However, it can also have the form of a prism or another body, provided that there is at least one predefined guide surface which is parallel to the desired insertion direction of the optical conductor. The base has a contact face, which is provided to make contact with the end of the flow cell. The contact face is preferably designed to be flat, as is the end of the flow cell. The imaginary axis of rotation of a partially rotationally symmetrical base in the application extends collinearly with respect to the tube axis in the area of the tube opening; the insertion movement of the connecting piece can take place along this axis of rotation.
Furthermore, the base has a reference face, which is intended to be arranged relative to the opening of the tube arranged in the end of the flow cell. In particular, the reference face can be a rotationally symmetrical face of the base, for example a section of a cylindrical outer face. The reference face is used to arrange the positioning means relative to the tube opening, so that the positioning means assumes a defined attitude relative to the tube in terms of position and alignment in space. The alignment and/or positioning of the connecting piece relative to the positioning means can likewise be carried out by means of this reference face but can also be achieved by other faces on the base with which the connecting piece interacts as it is inserted.
Furthermore, the base comprises an insertion region for the insertion of the connecting piece to be centered relative to the flow cell and to the tube. According to the invention, as a result of being pushed into the insertion region, the connecting piece is aligned by means of contact with the base (preferably with the reference face of the latter) and fixed in its radial position such that, as it is pushed further in, the optical conductor carried by the connecting piece is inserted centrally into the opening of the tube in the end of the flow cell. The positioning means thus constitutes a constructively simple but effective aid to the accurate and simple alignment of the connecting piece on the flow cell, it being assumed that the positioning means already inherently has an accurately predefined attitude relative to the tube opening when the connecting piece is pushed in.
According to a particularly expedient embodiment of the invention, the base is designed to be annular or tubular. The comparatively simple geometric shape is sufficient to achieve the desired centering effect. In particular, as a result of contact with the outer or inner circumferential surface of the annular or tubular base, the connecting piece can be aligned and positioned radially as it is inserted into the base. The contact face on the flange of the flow cell of an annular or tubular base is then formed by the front end of the ring or tube and has the form of a circular annular face.
In a modified embodiment of the base, the latter is formed as a pot structure. The pot likewise comprises an annular or tubular section but which is closed at one end by a bottom. The outer side of the bottom forms the contact face to make contact with the end of the flow cell, the bottom having a preferably central bottom opening for the optical conductor to be fed through and for the sample to be fed in, so that a connecting piece with the optical conductor inserted into the pot can project through the central opening. Given prior exact alignment of the positioning means relative to the tube opening in the end of the flow cell, the optical conductor comes centrally into the opening of the tube. The pot structure, as compared with the annular or tubular form, has the advantage of a greater contact area and/or offers more space for channels and cut-outs on the flange or on and in the pot bottom to receive adhesive material adjacent to the end of the flow cell. Such profiling of the contact face facing the end permits the specific local application of adhesive, preferably in the profile ways, in order for example to keep adhesive away from the tube or a sealing surface surrounding the latter and to offer compensation space for excess adhesive.
According to a further advantageous embodiment of the invention, the base is additionally equipped with guide means, which are intended to facilitate the insertion of the connecting piece. These can be chamfered areas at the end of the base facing away from the flow cell, which pre-center the connecting piece as it is inserted. As it is pushed further in, the connecting piece is then aligned accurately, preferably in contact with the reference face, within the scope of the selected manufacturing tolerances. In addition to the pre-centering, the guide means can also serve to fix a specific angle of rotation of the connecting piece about the tube axis, at which angle the latter is to be inserted into the base. This appears to be expedient in particular when the connecting piece is not formed completely rotationally symmetrically, for example when the sample feed leads laterally into the connecting piece.
In order to arrange the connecting piece relative to the flow cell with the aid of the positioning means, it is necessary to connect the positioning means to the flow cell and, in particular with reference to the opening of the tube, to align and/or to position said positioning means accurately in the end of the flow cell, the insertion direction being intended to coincide with the alignment of the tube axis. An appropriate method comprises the following method steps:
a) arranging the positioning means on the end of a flow cell;
b) positioning the positioning means with at least one reference face thereof relative to an opening present in the end of the flow cell;
c) fixing the positioning means on the flow cell in order to maintain the relative position established under b).
During the positioning according to method step b), the positioning means is positioned relative to the tube opening and optionally additionally aligned with respect to the angle of rotation about the tube axis. This is done in relation to the reference face of the positioning means. The base is displaced and positioned and/or aligned such that the at least one reference face assumes a predefined position and/or orientation relative to the tube opening. If this reference face is, for example, the cylindrical inner circumferential surface of a tubular base, then the reference face (and, with it, the base) would have to be aligned concentrically with respect to the tube opening. Of course, in this case it is assumed that the connecting piece guides its optical conductor likewise centrally and later, as it is inserted into the base, is aligned centrally on the latter.
The subsequent fixing of the positioning means on the end of the flow cell according to step c) can be carried out in particular by adhesive bonding; this is to be understood in the further sense as the production of an adhesive connection between base and flow cell, for example by using an adhesive. Alternatively, mechanical fixing means (screws, clamping elements, etc.) are suitable for fixing, it being necessary to factor in more overall space and mechanical stresses that may possibly occur. It is necessary to avoid undesired forces which could be introduced into the connection during the fixing, which impairs the previously undertaken alignment of the base on the flow cell. However, for repeated use, for example, the positioning means can also be connected detachably to the flow cell, for example in the form of a preferably multi-part pot structure, which is clamped on the flange or held on the flange by a union nut.
Expediently, the alignment of the base on the end of the flow cell can be carried out by using optical aids, for example with a microscope. Particularly suitable is the use of imaging computer means, in which the position of the base relative to the tube opening can be displayed well on a monitor.
In step a), the flow cell having the tube opening could be moved, by using the optical aid (for example a camera, monitor or image-processing software), into an intended position, in which the center of the tube opening appears in the center of the monitor, for example. For this purpose, a camera could be directed at the end, approximately perpendicular thereto. In a following step, the base is then placed on the end and displaced in such a way that, in the image thereof on the monitor, its reference face accurately assumes the desired relative position and/or alignment with respect to the tube opening. In addition, the orientation of the rotary attitude of the base relative to the flow cell is set in this way, in order for example to move a cut-out in the wall of the base into the correct position. An adhesive previously or subsequently arranged between base and flow cell can then be cured, the reference face of the positioning means preferably being fixed centrally in relation to the tube opening. During the subsequent insertion of the connecting piece, the optical conductor held centrally by the latter then preferably comes centrally into the tube opening. The use of optical aids permits a magnified pictorial representation of the individual components, which is useful for the precise alignment, given the small dimensions of the tube opening and of the optical conductor. In addition, the aforementioned displacement can be carried out in an aid constructed specifically for this purpose, for example by using micrometer screws, or even automatically by using the data from the camera. This increases the accuracy and reproducibility of the method. In the above-described method, the end of the flow cell with the tube opening located flush therein is further removed from the camera than the base subsequently placed on the end of the flow cell, which, with the end thereof facing away from the flow cell, is then located closer to the camera or the lens of the latter. During the subsequent optical adjustment of the base relative to the tube opening, it is therefore possible for the problem to arise that the base with its reference face and the tube opening then located somewhat further away cannot be depicted sharply at the same time, which makes the optical adjustment more difficult. In order to overcome this problem, a special embodiment of the method provides that, before the arrangement of the flange of the flow cell under the camera, a plane-parallel lens or a similar optical aid is introduced into the optical path between flange and camera, in order to lengthen this path (for example a BK7 lens). Following the focusing of the camera onto the flange surface through the lens, the flange can be displaced such that the tube opening imaged by the camera reaches an intended position of the image, for example the center of the image.
For the subsequent arrangement of the base relative to the flow cell and tube opening, said base is placed on the end of the flange of the flow cell. Following removal of the lens from the optical path, the camera (without active re-focusing by the camera itself) focuses at another depth which, given suitable selection of the lens, coincides accurately with the attitude and height of the reference face. This could be, for example, the upper side of a bottom of the base, which faces the camera and which, for example, is 1 mm thick and is therefore located 1 mm closer to the camera than the end of the flow cell flange. The base and therefore the image of its now sharply imaged reference face can likewise be displaced into the center of the image, which means that the desired arrangement of the base relative to the flow cell is carried out particularly simply and without re-setting the camera.
In order to increase the accuracy of the relative positioning, the steps of arranging the flange (with use of the lens) and of the base (without use of the lens) can be repeated, in order respectively to eliminate final deviations from the center of the image.
Following the formation of the adhesive connection between base and flow cell, the positioning means can reliably and accurately align the connecting piece that is to be inserted subsequently as it is inserted.
The invention is to be explained in more detail below by using exemplary Figures.
A substantially rotationally symmetrical positioning means 1 is illustrated in
In
The contact face 7 of the base 2 adjoins the end of a flow cell 10, shown only partly in
Via the grooves 12, illustrated in
Number | Date | Country | Kind |
---|---|---|---|
10 2013 102 440.8 | Mar 2013 | DE | national |