POWRE: Techniques for Investigating Cytoskeletal Effects on Recombinant Protein Glycosylation

Information

• NSF Award
• 0083203

Owner

• ALBANY MEDICAL COLLEGE

• Award Id

  0083203

• Award Effective Date

  9/1/2000 - 24 years ago

• Award Expiration Date

  12/31/2001 - 22 years ago

• Award Amount

  $ 104,873.00

• Award Instrument

  Standard Grant

The goal of this project is to investigate the effects of culture conditions on recombinant protein production, specifically the effects of cell adhesion on yield and glycosylation patterns of recombinant proteins produced in Chinese hamster ovary (CHO) cells. The specific goals for the POWRE project are: (1) to develop expertise in the techniques for studying protein glycosylation including high performance liquid chromatography and gel electrophoresis; (2) to determine what probes and imaging techniques are best suited for studies of the cytoskeleton for suspension and adherent cultures of CHO cells; and (3) to obtain preliminary data analyzing the glycoforms of tissue plasminogen activator produced under the different culture conditions and determine which of the techniques for studying protein glycosylation will be best suited for future studies.

• Program Officer

  Fred G. Heineken

• Min Amd Letter Date

  8/23/2000 - 24 years ago

• Max Amd Letter Date

  8/23/2000 - 24 years ago

• ARRA Amount

• Name

  Albany Medical College of Union University

• City

  Albany

• State

  NY

• Country

  United States

• Address

  47 New Scotland Avenue

• Postal Code

  122083479

• Phone Number

  5182625181

• First Name

  Susan

• Last Name

  Sharfstein

• Email Address

  ssharfstein@sunypoly.edu

• Start Date

  8/23/2000 12:00:00 AM

• Name

  Industrial Technology

• Code

  308000

• Text

  SPECIAL STUDIES AND ANALYSES

• Code

  1385

• Text

  BIOCHEMICAL & BIOMASS ENG

• Code

  1402

• Text

  PROF OPPOR FOR WOMEN IN RSCH

• Code

  1592

• Text

  EXP PROG TO STIM COMP RES

• Code

  9150

• Text

  BIOPROCESSING/BIOMOLECULAR MATERIALS

• Code

  9181

• Text

  BIOTECHNOLOGY

• Text

  BIOTECH, BIOCHEM & BIOMASS ENG

• Code

  1491