Claims
- 1. A protein database comprising nonhomologus proteins having identified single nucleotide polymorphisms that are relevant to the function of the protein.
- 2. The database of claim 1, wherein the function is binding of the protein.
- 3. The database of claim 1, wherein the database is determined by a computational method comprising the step of determining the residue-specific connectivity between residues in the protein according to the equation
- 4. The database of claim 1, wherein the database is determined by a computational method comprising the step of determining the functional connectivity between the binding site of the protein and every residue in the protein according to the equation
- 5. A method of predicting a subset of the population at risk for an adverse side effect of a pharmaceutical composition comprising using the database of claim 1 to determine the subset having a relevant single nucleotide polymorphism interfering with an active site of a protein.
- 6. A method of targeting a pharmaceutical composition for an active site of a protein comprising using the database of claim 1 to determine the interactions of single nucleotide polymorphisms on the active site of a protein and designing the pharmaceutical composition to overcome the interactions of the single nucleotide polymorphism so that the pharmaceutical composition binds to the active site of the protein.
- 7. A method of developing a protein database comprising the steps of:
inputting high resolution structures of proteins; generating an ensemble of incrementally different conformational states by combinatorial unfolding of a set of predefined folding units in all possible combinations of each protein; determining the probability of each said conformational state; calculating a residue-specific connectivity of each said conformational state; and calculating a functional connectivity of each of said conformation state.
- 8. A system for developing a protein database having identified single nucleotide polymorphisms that are relevant to the function of the protein comprising
a protein database having a data structure for protein data, said data structure including data fields for relevant single nucleotide polymorphisms; and a computer-based program for identifying protein data for said database, said program having
an input module for receiving high resolution structure data for one or more proteins, and a processing module for determining the relevance of single nucleotide polymorphisms in the active site of one or more proteins and storing said data into said data fields of said protein database.
- 9. The system of claim 8, wherein said computer program further includes a display module for producing one or more graphical reports to a screen or a print-out.
- 10. A database having a data structure which stores information defining relevant single nucleotide polymorphism groups, said database comprising:
a field for storing a value of an amino acid name or amino acid abbreviation; and
one or more classification fields for storing a value representing a numerical value for a relevant single nucleotide polymorphism.
- 11. A method of designing a protein pharmaceutical exhibiting optimized pharmaceutical properties comprising the steps of:
i. obtaining a test data set of variants of the protein pharmaceutical, wherein the variants comprise single nucleotide polymorphisms; ii. preparing a library of ensemble derived properties for the test data set using a computer based method; iii. obtaining experimental data for a given property for each protein variant within the test data set; iv. deriving a parametric equation using the experimental data and the library of ensemble derived properties; and v. creating a protein pharmaceutical using the information obtained by the above steps to provide optimized pharmaceutical properties.
- 12. The method of claim 11, wherein the optimized property is increased binding affinity.
- 13. A method of identifying relevant single nucleotide polymorphisms comprising the steps of:
inputting high resolution structures of proteins; generating an ensemble of incrementally different conformational states by combinatorial unfolding of a set of predefined folding units in all possible combinations of each protein; determining the probability of each said conformational state; calculating a residue-specific connectivity of each said conformational state; and calculating a functional connectivity of each of said conformation state.
Parent Case Info
[0001] This application claims priority to U.S. Provisional Application No. 60/382,784 filed May 23, 2002, which is incorporated herein by reference.
Government Interests
[0002] The work herein was supported by grants from the United States Government. The United States Government may have certain rights in the invention.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60382784 |
May 2002 |
US |