Patent Application
20240268439
This application claims the priority benefit of China application serial no. 202310044315.5, filed on Jan. 30, 2023. The entirety of the above-mentioned patent application is hereby incorporated by reference herein and made a part of this specification.
The present disclosure relates to a preparation method for a diet-reducing capsule containing multi-nutrient microspheres, and a resulting product, belonging to the field of food biotechnology.
Vitamins, minerals and probiotics play different and important roles in the metabolism, growth, development and maintenance of physical health of the human body, so daily supplementation of these substances is crucial. However, some vitamins and probiotics will lose their activity in the environments of gastric acid and bile salts due to their special properties. In addition, according to national regulations, nutritional supplement products can play a role in health care in need of reaching an amount required by the human body within their shelf life, so vitamins and probiotics need to be protected.
Moreover, due to the improvement of living standards of people in China, some of them have unbalanced and unreasonable living and dietary habits, resulting in increasingly serious obesity problems, so diseases that seriously endanger human health, such as hypertension, type II diabetes, cardiovascular and cerebrovascular diseases, and some cancers have followed. Therefore, weight control is key means to prevent and delay the onset and progression of these diseases.
At present, a number of regimens have been developed for weight management, often including a lifestyle intervention method, a drug intervention method, and a surgical intervention method. However, the lifestyle intervention method is easy to give up halfway, and the drug intervention method and surgical intervention method have great side effects, which violate the principle of health. Fundamentally, weight loss occurs when consumption is higher than intake, but increasing consumption requires a lot of exercise, which is difficult for stressed people who do not like sports, so it is especially important to invent a healthy way to lose weight with the goals of reducing intake and controlling diet.
Some measures have been taken to address the problem of controlling diet to lose weight. The patent CN113975335 B uses a green coffee bean extract, a Hibiscus sabdariffa extract, a Crocus sativus L. extract and a L-carnitine as raw materials to prepare a composition that controls appetite and induces satiety, and induces an increase in human glucagon-like peptide-1 (GLP-1) secretion through the regulation of the feeding center of the brain-gut axis, thereby affecting the satiety center and achieving the purpose of weight loss. However, in this method, the increase in GLP-1 may cause side effects such as palpitations in obese patients, and may also cause excessive stress on GLP-1-secreting organs, resulting in premature aging or other potential risks. The patent CN102905762 B provides a methylcellulose gel mass that can provide satiety, but cannot meet daily nutritional needs of the human body because of the lack of vitamins and minerals, and can also cause physiological dysfunction due to the long-term lack of vitamins and minerals, so the loss outweighs the gain. Meanwhile, the intestinal flora of obese patients may be different from that of normal people, which may also be the cause of obesity. It is worth noting that weight loss is a long-term process, and if there are no hints of words such as “vitamins”, “minerals” and “health”, it may lead to obese people only caring about weight and ignoring health, which is not conducive to the development of good dietary habits. Therefore, the present disclosure aims to develop a diet-reducing capsule that supplements a variety of nutrients at the same time.
An object of the present disclosure is to provide a preparation method for a die-reducing capsule (i.e., food intake reducing and dieting capsule) that can disintegrate rapidly and simultaneously achieve healthy weight loss, nutritional supplementation, intestinal conditioning and increased colonization rate of probiotics, and the diet-reducing capsule prepared according to this method.
A specific technical solution of the present disclosure is as follows.
A preparation method for a diet-reducing capsule containing multi-nutrient microspheres includes the following steps:
Further, in step (1), the sodium carboxymethyl cellulose and the polybasic carboxylic acid are cross-linked to form a three-dimensional network structure, wherein the sodium carboxymethyl cellulose has a viscosity of 7000 to 15000.
Further, in step (1), the polybasic carboxylic acid is one of citric acid, aconitic acid, oxalic acid, tartaric acid, malic acid, acetic acid, malonic acid, succinic acid, adipic acid, azelaic acid, terephthalic acid, trimellitic acid, trimesic acid, ethylenediaminetetraacetic acid and 2-methylglutaric acid, preferably citric acid.
Further, in step (1), a viscosity of the sodium carboxymethyl cellulose is 7000 to 15000; a mass ratio of the sodium carboxymethyl cellulose to the polybasic carboxylic acid is (310 to 350): 1, and a mass ratio of the sodium carboxymethyl cellulose to water is 1:(10 to 22).
Further, in step (1), a way of stirring is performed first for 80 to 100 min at a rotation speed of 50 to 70 rpm, and then for 14 to 20 h at a rotation speed of 20 to 40 rpm; and a drying way of the drying oven is to adjust the temperature of the drying oven to 40 to 60° C., with drying first for 20 to 28 h, turning over and then continuing to dry for 28 to 36 h.
Further, in step (1), high-temperature cross-linking is to treat the dried gel at high temperature, wherein a temperature of high-temperature cross-linking is 110 to 130° ° C., and the high-temperature cross-linking time is 3.6 to 4.4 h.
Further, in step (1), a way of crushing and sieving is to crush the cross-linked product with a crusher, and then sieve with an 18-mesh sieve and a 26-mesh sieve; and a way of washing with the distilled water is to wash the solid hydrogel particles with the distilled water for 2 to 6 times, 2 to 4 h each time, wherein a mass ratio of the solid hydrogel particles to the distilled water is 1:(100 to 200) in each washing.
Further, in step (2), the probiotics is a mixed strain selected from two or more of Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus helveticus, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus crispatus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus, Lactobacillus casei subsp. Casei, Lactobacillus paracasei, Lactobacillus reuteri, Bifidobacterium lactis, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium adolescent, Bifidobacterium animalis and Streptococcus thermophilus, preferably Bifidobacterium longum and Lactobacillus acidophilus.
Further, in step (2), the natural polymer material is one or more of sodium alginate, chitosan, modified starch, carboxymethyl cellulose, methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, gallant gum, k-carrageenan, Arabic gum, pectin, carrageenan, gellan gum, xanthan gum, maltodextrin, β-cyclodextrin, gelatin, soy protein isolate and whey protein, preferably sodium alginate, chitosan, and gellan gum, with a ratio of sodium alginate to chitosan to gellan gum of 40:3:5 in parts by weight.
Further, in step (2), the freeze-drying protective agent is one or more of soluble starch, hydroxyethyl starch, resistant dextrin, fructose, glucose, lactose, sucrose, ribose, rhamnose, galactose, fucose, mannose, arabinose, xylan, skimmed milk powder, glycerin, lactitol, sorbitol, mannitol, xylitol, erythritol, maltitol, sodium glutamate, antifreeze peptide, sericin peptide, fish collagen peptide, collagen, and polyvinylpyrrolidone, preferably soluble starch, skimmed milk powder, glycerin and xylan, with a ratio of soluble starch to skimmed milk powder to glycerin to xylan of 5:6:1:10 in parts by weight.
Further, in step (2), an inoculation amount of the probiotics is 1.5 to 4.5% in the mass of the sterile medium; and the sterile medium is an MRS liquid medium.
Further, in step (2), an activation method for the probiotics may be operated by a method disclosed in the prior art, and the low-temperature centrifugation is to carry out centrifugation on the sterile medium inoculated with the probiotics at 3 to 5° C., with a centrifugation speed of 3500 to 5500 rpm, and centrifugation time of 10 to 20 min.
Further, in step (2), the bacterial sludge is washed with the sterile normal saline for 1 to 3 times, and a mass concentration of the sterile normal saline is 0.85% to 0.95%.
Further, in step (2), in the aqueous solution of the freeze-drying protective agent, a mass fraction of the freeze-drying protective agent is 6% to 20%.
Further, in step (2), the bacterial sludge and the aqueous solution of the freeze-drying protective agent are mixed in a volume ratio of 1:(3 to 5), and then stirred for 10 to 20 min at a stirring speed of 200 to 400 rpm.
Further, in step (2), the probiotics in the bacterial suspension has a concentration of 109 CFU/mL.
Further, in step (2), a mass concentration of the aqueous solution of the natural polymer material is 0.5% to 1.5%, and a volume ratio of the bacterial suspension to the aqueous solution of the natural polymer material is 1:(0.5 to 1.5).
Further, in step (2), the probiotics is solidified with a 0.1 mol/L CaCl2) solution for 20 to 40 min.
Further, in step (2), the low-temperature storage temperature is 3 to 5° C.
Further, in step (3), the vitamin is at least one of vitamin A, vitamin D3, vitamin E, vitamin K2, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin B13, vitamin B15, vitamin C, biotin, niacinamide, folic acid, inositol, and pantothenic acid; the mineral is at least one of calcium, magnesium, manganese, iron, zinc, cobalt, molybdenum, chromium, copper, selenium, iodine, phosphorus, potassium, sodium, sulfur, and chlorine, preferably, calcium, magnesium, manganese, iron, zinc, selenium and copper; the prebiotics is one of fructooligosaccharides, xylooligosaccharides, galactooligosaccharides, isomaltooligosaccharides, soybean oligosaccharides, mannose oligosaccharides, lactulose, raffinose, stachyose, chitosan oligosaccharides, resistant starch, wheat dextrin, inulin, polydextrose, trehalose, Aspergillus niger oligosaccharides, spirulina, Arthrospira, chlorella, and microalgae, preferably fructooligosaccharides.
In a specific example of the present disclosure, in step (3), the vitamins include vitamin A, vitamin D3, vitamin E, vitamin K2, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacinamide, folic acid, vitamin C, and pantothenic acid; and the minerals include calcium carbonate, magnesium gluconate, manganese sulfate, ferrous lactate, zinc gluconate, sodium selenite, and copper sulfate. The contents of these vitamins and minerals in the vitamin-mineral-prebiotics-natural polymer material mixed solution are: 95 to 128 μg/g of vitamin A, 1 to 6 μg/g of vitamin D3, 1 to 6 mg/g of vitamin E, 6 to 10 μg/g of vitamin K2, 0.1 to 0.6 mg/g of vitamin B1, 0.1 to 0.6 mg/g of vitamin B2, 0.1 to 0.6 mg/g of vitamin B6, 0.1 to 0.7 μg/g of vitamin B12, 1 to 7 mg/g of niacinamide, 40 to 80 μg/g of folic acid, 10 to 40 mg/g of vitamin C, and 0.5 to 2.5 mg/g of pantothenic acid; and 93 to 133 mg/g of calcium carbonate, 27 to 51 mg/g of magnesium gluconate, 0.58 to 0.98 mg/g of manganese sulfate, 1 to 5 mg/g of ferrous lactate, 0.1 to 2.5 mg/g of zinc gluconate, 10 to 17 μg/g of sodium selenite, and 0.01 to 0.30 mg/g of copper sulfate.
Further, in step (3), the content of the prebiotics in the vitamin-mineral-prebiotics-natural polymer material mixed solution is 0.3 to 1.0 g/100 mL.
Further, in step (3), the natural polymer material is one or more of sodium alginate, chitosan, modified starch, carboxymethyl cellulose, methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, gallant gum, k-carrageenan, Arabic gum, pectin, carrageenan, gellan gum, xanthan gum, maltodextrin, ß-cyclodextrin, gelatin, soy protein isolate, and whey protein, preferably sodium alginate, chitosan and gellan gum, with a ratio of sodium alginate to chitosan to gellan gum of 40:3:5 in parts by weight.
Further, in step (3), the vitamin-mineral-prebiotics composite powder and the aqueous solution of the natural polymer material are mixed in a mass ratio of (1 to 2):11.
Further, in step (3), a mass concentration of the aqueous solution of the natural polymer material is 2% to 4%.
Further, in step (3), the vitamin-mineral-prebiotics composite powder and the aqueous solution of the natural polymer material are mixed for 10 to 30 min at a rotation speed of 150 to 350 rpm.
Further, in step (4), the enteric coating material is one or more of shellac, algin, diclofenac, acrylic resin No. I, acrylic resin No. II, acrylic resin No. III, cellulose acetate benzenedicarboxylate, cellulose acetate succinate, hydroxypropyl methylcellulose acetate succinate, cellulose acetate phthalate, hydroxypropyl methylcellulose phthalate, cellulose acetate 1,2,4-benzenetricarboxylate, hydroxypropyl methylcellulose 1,2,4-benzenetricarboxylate, hydroxypropyl methylcellulose titanate, and polyvinyl acetate phthalate, preferably hydroxypropyl methylcellulose phthalate.
Further, in step (4), the mixing time of the solidified probiotics and the vitamin-mineral-prebiotics-natural polymer material mixed solution is 10 to 30 min, with a rotation speed of 100 to 300 rpm; and the mixing time of the mixed nutrient solution and the aqueous solution of the enteric coating material is 10 to 30 min, with a rotation speed of 100 to 300 rpm.
Further, in step (4), a mass concentration of the aqueous solution of the enteric coating material is 4% to 12%.
Further, in step (4), the washing is performed with the sterile distilled water for 2 to 4 times.
Further, in step (4), the solidified probiotics and the vitamin-mineral-prebiotics-natural polymer material mixed solution are mixed in a mass ratio of 1:(2 to 4).
Further, in step (4), the mixed nutrient solution and the aqueous solution of the enteric coating material are mixed in a mass ratio of 1:(4 to 6).
Further, in step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres and the wet hydrogel particles are mixed in a mass ratio of 1:(13 to 17).
Further, in step (5), the mixing time of the wet probiotics-prebiotics-vitamin-mineral microspheres and the wet hydrogel particles is 10 to 20 min, with a rotation speed of 30 to 60 rpm.
Further, in step (5), the pre-cooling treatment is performed at −80° C. for 1 to 4 h; and the freeze-drying conditions are as follows: a temperature is −55° C., a vacuum degree is 25 Pa, and the time is 24 to 48 h.
Further, in step (6), the laser source used in the process of punching with laser is a cold light source, the punched hole has an aperture of 0.5 to 1.5 mm, and the number of the punched holes is 1 to 4.
Further, in step (6), the empty capsule shell is made of one of gelatin, pullulan and glutinous rice starch, preferably gelatin. The empty capsule shell has a model selected from one of 000 #, 00 #, 0 #, 1 #, 2 #, 3 #, 4 # and extended models thereof, preferably 00 # or the extended model thereof.
Further, in step (6), a specific position of the empty capsule shell is punched, wherein the specific position may be both ends of the empty capsule shell, or the waist of the empty capsule shell, or a side wall of the empty capsule shell, or a combination of these positions. Preferably, the punching position may be one of “symmetrical positions in the middle of the waist”, “central symmetrical positions at both ends”, “equidistant symmetrical positions at both ends and the waist”, and “equidistant positions of skew symmetry from the vertex and midline”. The symmetrical punching positions in the middle of the waist mean that the middle position on the waist of the empty capsule shell is punched; and the punched holes are distributed in axial symmetry along a central axis of the capsule (as shown in
Further, in step (6), when the porous capsule shell is combined with the capsule content, a mass of the capsule content contained in each porous capsule shell is 0.60 to 0.75 g.
Further, the components used in the present disclosure are commercially available products, and their structures and compositions are known to those skilled in the art.
The diet-reducing capsule prepared according to the method of the present disclosure includes a porous capsule shell and a capsule content, wherein the capsule content is microspheres containing multiple nutrients (as shown in
The present disclosure has the following beneficial effects.
(1) The present disclosure organically integrates the four major problems of weight management, intestinal flora regulation, trace organic matter supply and trace element supply together, and develops this diet-reducing capsule. This diet-reducing capsule can play a variety of health care functions at the same time and ensure the effectiveness of time, is more conducive to physical recovery and helps people develop good life habits. In addition, no any other toxic substances or strong oxidizing chemical components is added in the present disclosure, so the prepared diet-reducing capsule is safe and healthy.
(2) The hydrogel particles in the present disclosure can be swollen by absorbing water, occupy a certain volume in the stomach, and form the satiety, so as to reduce the food intake and achieve the purpose of weight loss. The hydrogel particles in the present disclosure have similar mechanical properties as normal vegetables after absorbing water and swelling, and are finally excreted with the formation of feces from food residues. In addition, the administration dosage (within a dosage range) may be adjusted in the present disclosure according to personal circumstances (e.g., appetite) to achieve personalized weight loss. Meanwhile, the intake of vitamins and minerals prevents the development of certain diseases, especially those caused by malnutrition due to reduced food intake, while the probiotics help to regulate the intestines and restore normal physiological functions of flora.
(3) The microspheres containing multiple nutrients of the present disclosure have the following advantages: A, the enteric coating material is added to make the microspheres have the enteric solubility, which can effectively resist the damages from gastric acid and bile salts; the freeze-drying protective agent is added to reduce the appearance of micro ice crystals in the freeze-drying process of the microspheres, and reduce the probability of micro ice crystals damaging probiotic bacteria; and the prebiotics is added to not only provide nutrients for the intestinal colonization of probiotics, but also be directly absorbed by the human body, increasing the nutritional value. B. The natural polymer materials package the prebiotics, the vitamins and the minerals to reduce their direct contact with the probiotics, and avoid adverse effects such as reduced efficacy caused by the interaction of the prebiotics, the vitamins, the minerals and the probiotics. C. The microspheres prepared by the method of the present disclosure have a number of viable bacteria up to 108 CFU/g, a probiotics embedding rate of more than 80%, a vitamin embedding rate of more than 80%, a mineral embedding rate of more than 70%, and a prebiotics embedding rate of more than 75%. Such microspheres can effectively improve the acid resistance, the salt resistance and the long-term storage of the probiotics, and also significantly improve a low survival rate of the probiotics after freeze-drying.
(4) According to the present disclosure, the wet hydrogel particles and the wet multi-nutrient microspheres (i.e., wet probiotics-prebiotics-vitamin-mineral microspheres) are mixed and then freeze-dried. With such process, the microspheres can be attached to the surfaces of the hydrogel particles or be entrained among the hydrogel particles to provide a brief stable microenvironment for the presence of the microspheres, and also prepare for the next release of the microspheres, which is conducive to improving the utilization rates of the prebiotics, the probiotics, the vitamins and the minerals, as well as their absorption and metabolism in the body.
(5) The present disclosure introduces a laser punching technology to punch small holes in a specific position of the capsule shell, and an aperture range is set based on a standard that the capsule content will not escape. This change in the appearance of the capsule shell may not have any adverse effects on safety and efficacy issues, but can speed up the dissolution of gastric juice to the capsule shell, thereby accelerating the release of the capsule content, and ultimately making the satiety more timely, which can effectively improve the user's enthusiasm and compliance, generate a sense of trust, enhance the self-confidence, and be more conducive to the formation of their own good life habits.
(6) The preparation method of the diet-reducing capsule of the present disclosure is simple to operate, economical and easy to implement, and is suitable for industrial production.
In
indicates that there is a hole in the front of the empty capsule shell,
indicates that there is a hole in the back of the empty capsule shell, and
indicates that there is a hole in the side of the empty capsule shell.
The present disclosure will be further described in detail below in conjunction with specific examples, comparative examples and accompanying drawings.
In the following examples and comparative examples, unless otherwise specified, the concentrations are all in percentage by mass.
In the following examples and comparative examples, unless otherwise specified, all sterilization operations in a sterile aqueous solution, an MRS medium, etc. are carried out by a moist heat sterilization method, wherein a sterilization temperature is 121° C. and the sterilization time is 20 min.
In the following examples and comparative examples, unless otherwise specified, in step (2), the probiotics is all mixed flora composed of Bifidobacterium longum and Lactobacillus acidophilus; the freeze-drying protective agents are soluble starch, skimmed milk powder, glycerol and xylan (a ratio of soluble starch to skimmed milk powder to glycerol to xylan is 5:6:1:10 in parts by weight), and a concentration of the probiotics in the bacterial suspension is 109 CFU/mL; in steps (2) and (3), the natural polymer materials are sodium alginate, chitosan and gellan gum (a ratio of sodium alginate to chitosan to gellan gum is 40:3:5 in parts by weight); in step (3), the prebiotics is fructooligosaccharides; in step (4), the enteric coating material is hydroxypropyl methylcellulose phthalate; and in step (6), all the empty capsule shells are made of gelatin, all in 00 # type, and a mass of the capsule content contained in the diet-reducing capsule is 0.75 g/capsule.
Step (1), sodium carboxymethyl cellulose (a viscosity of 11000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 330:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:16) to obtain a mixture; the mixture was stirred at 60 rpm for 90 min, and then stirred for at 30 rpm 16 h to obtain a gel; the gel was dried in a drying oven at 45° C. for 24 h, turned over and continued to dry for 32 h, then cross-linked at high temperature of 120° C. for 4 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed with distilled water for 4 times, 3 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:150 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 3%, and repeatedly activated for 5 generations under the same culture conditions (36.5° C., 24 h); bacterial sludge was collected by low-temperature centrifugation (4° C., 4500 rpm, 15 min), and washed twice with 0.9% sterile normal saline; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 13% according to a volume ratio of 1:4 to obtain a bacterial suspension; the bacterial suspension was then mixed evenly with an aqueous solution of a natural polymer material having a concentration of 1% according to a volume ratio of 1:1 to obtain a mixed solution; the obtained mixed solution was then solidified with a 0.1 mol/L CaCl2) solution for 30 min, washed and filtered to obtain solidified probiotics; the solidified probiotics was stored at a low temperature of 4ºC for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (250 rpm, 20 min) evenly with the aqueous solution of the natural polymer material having a concentration of 3% according to a mass ratio of 1.5:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 111 μg/g of vitamin A, 4 μg/g of vitamin D3, 4 mg/g of vitamin E, 8 μg/g of vitamin K2, 0.4 mg/g of vitamin B1, 0.4 mg/g of vitamin B2, 0.4 mg/g of vitamin B6, 0.4 μg/g of vitamin B12, 4 mg/g of niacinamide, 60 μg/g of folic acid, 25 mg/g of vitamin C, and 1.5 mg/g of pantothenic acid; 113 mg/g of calcium carbonate, 39 mg/g of magnesium gluconate, 0.78 mg/g of manganese sulfate, 3 mg/g of ferrous lactate, 1.5 mg/g of zinc gluconate, 14 μg/g of sodium selenite, and 0.16 mg/g of copper sulfate; and the prebiotics content was 0.65 g/100 mL;
Step (4), the solidified probiotics was mixed (200 rpm, 20 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:3 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed with an aqueous solution of an enteric coating material having a concentration of 8% according to a mass ratio of 1:5, washed for 3 times and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (45 rpm, 15 min) evenly with the wet hydrogel particles according to a mass ratio of 1:15 to obtain a mixture; the mixture was then pre-cooled at −80° C. for 2.5 h, and then freeze-dried at −55° ° C. for 36 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with two holes, each having an aperture of 1 mm, in symmetrical positions in the middle of the waist with laser (as shown in
Step (1), sodium carboxymethyl cellulose (a viscosity of 15000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 350:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:10) to obtain a mixture; the mixture was first stirred at 70 rpm for 100 min, and then stirred at 40 rpm for 20 h to obtain a gel; the gel was dried in a drying oven at 60° C. for 28 h, turned over and continued to dry for 36 h, then cross-linked at high temperature of 125° C. for 4.4 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed with distilled water for 6 times, 4 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:200 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 4.5%, and repeatedly activated for 5 generations under the same culture conditions (38° C., 27 h); bacterial sludge was collected by low-temperature centrifugation (5° C., 5500 rpm, 20 min), and washed with 0.95% sterile normal saline for 3 times; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 20% according to a volume ratio of 1:5 to obtain a bacterial suspension; the bacterial suspension was then mixed evenly with an aqueous solution of a natural polymer material having a concentration of 1.5% according to a volume ratio of 1:1.5 to obtain a mixed solution; the obtained mixed solution was then solidified with a 0.1 mol/L CaCl2) solution for 40 min, washed and filtered to obtain solidified probiotics; the solidified probiotics was stored at a low temperature of 5° ° C. for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (350 rpm, 30 min) evenly with the aqueous solution of the natural polymer material having a concentration of 4% according to a mass ratio of 2:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 128 μg/g of vitamin A, 6 μg/g of vitamin D3, 6 mg/g of vitamin E, 10 μg/g of vitamin K2, 0.6 mg/g of vitamin B1, 0.6 mg/g of vitamin B2, 0.6 mg/g of vitamin B6, 0.7 μg/g of vitamin B12, 7 mg/g of niacinamide, 80 μg/g of folic acid, 40 mg/g of vitamin C, and 2.5 mg/g of pantothenic acid; 133 mg/g of calcium carbonate, 51 mg/g of magnesium gluconate, 0.98 mg/g of manganese sulfate, 5 mg/g of ferrous lactate, 2.5 mg/g of zinc gluconate, 17 μg/g of sodium selenite, and 0.3 mg/g of copper sulfate; and the prebiotics content was 1.0 g/100 mL;
Step (4), the solidified probiotics was mixed (300 rpm, 30 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:4 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed evenly with an aqueous solution of an enteric coating material having a concentration of 8% according to a mass ratio of 1:6, washed for 4 times and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (60 rpm, 20 min) evenly with the wet hydrogel particles according to a mass ratio of 1:17 to obtain a mixture; the mixture was then pre-cooled at −80° ° C. for 4 h, and then freeze-dried at −55° C. for 48 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with two holes, each having an aperture of 1 mm, in symmetrical positions in the middle of the waist with laser to obtain a porous capsule shell; and the porous capsule shell was then combined with the capsule content to obtain the diet-reducing capsule containing multi-nutrient microspheres.
Step (1), sodium carboxymethyl cellulose (a viscosity of 7000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 310:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:22) to obtain a mixture; the mixture was first stirred at 50 rpm for 80 min, and then stirred at 20 rpm for 14 h to obtain a gel; the gel was dried in a drying oven at 40° C. for 20 h, turned over and continued to dry for 28 h, then cross-linked at high temperature of 110° C. for 3.6 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed twice with distilled water, 2 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:100 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 1.5%, and repeatedly activated for 5 generations under the same culture conditions (35° C., 21 h); bacterial sludge was collected by low-temperature centrifugation (3° C., 3500 rpm, 10 min), and washed once with 0.85% sterile normal saline; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 6% according to a volume ratio of 1:3 to obtain a bacterial suspension; the bacterial suspension was then mixed evenly with an aqueous solution of a natural polymer material having a concentration of 0.5% according to a volume ratio of 1:0.5 to obtain a mixed solution; the obtained mixed solution was then solidified with a 0.1 mol/L CaCl2) solution for 20 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a low temperature of 3° C. for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (150 rpm, 10 min) evenly with the aqueous solution of the natural polymer material having a concentration of 3% according to a mass ratio of 1:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 95 μg/g of vitamin A, 1 μg/g of vitamin D3, 1 mg/g of vitamin E, 6 μg/g of vitamin K2, 0.1 mg/g of vitamin B1, 0.1 mg/g of vitamin B2, 0.1 mg/g of vitamin B6, 0.1 μg/g of vitamin B12, 1 mg/g of niacinamide, 40 μg/g of folic acid, 10 mg/g of vitamin C, and 0.5 mg/g of pantothenic acid; the minerals include 93 mg/g of calcium carbonate, 27 mg/g of magnesium gluconate, 0.58 mg/g of manganese sulfate, 1 mg/g of ferrous lactate, 0.1 mg/g of zinc gluconate, 10 μg/g of sodium selenite, and 0.01 mg/g of copper sulfate; and the prebiotics content was 0.3 g/100 mL;
Step (4), the solidified probiotics was mixed (100 rpm, 10 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:2 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed evenly with an aqueous solution of an enteric coating material having a concentration of 8% according to a mass ratio of 1:4, washed twice and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (30 rpm, 10 min) evenly with the wet hydrogel particles according to a mass ratio of 1:13 to obtain a mixture; the mixture was then pre-cooled at −80° C. for 1 h, and then freeze-dried at −55° C. for 24 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with two holes, each having an aperture of 1 mm, in central symmetrical positions at both ends of the entire shell with laser to obtain a porous capsule shell; and the porous capsule shell was then combined with the capsule content to obtain the diet-reducing capsule containing multi-nutrient microspheres.
Step (1), sodium carboxymethyl cellulose (a viscosity of 11000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 350:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:10) to obtain a mixture; the mixture was first stirred at 60 rpm for 90 min, and then stirred at 40 rpm for 20 h to obtain a gel; the gel was dried in a drying oven at 40° ° C. for 20 h, turned over and continued to dry for 28 h, then cross-linked at high temperature of 120° ° C. for 4 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed with distilled water for 6 times, 4 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:200 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 1.5%, and repeatedly activated for 5 generations under the same culture conditions (36.5° C., 24 h); bacterial sludge was collected by low-temperature centrifugation (5° C., 5500 rpm, 20 min), and washed once with 0.85% sterile normal saline; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 13% according to a volume ratio of 1:3 to obtain a bacterial suspension; the bacterial suspension was then mixed evenly with an aqueous solution of a natural polymer material having a concentration of 1% according to a volume ratio of 1:0.5 to obtain a mixed solution; the obtained mixed solution was then solidified with a 0.1 mol/L CaCl2) solution for 30 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a low temperature of 4° C. for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (250 rpm, 20 min) evenly with the aqueous solution of the natural polymer material having a concentration of 2% according to a mass ratio of 1:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 111 μg/g of vitamin A, 4 μg/g of vitamin D3, 4 mg/g of vitamin E, 8 μg/g of vitamin K2, 0.4 mg/g of vitamin B1, 0.4 mg/g of vitamin B2, 0.4 mg/g of vitamin B6, 0.4 μg/g of vitamin B12, 4 mg/g of niacinamide, 60 μg/g of folic acid, 25 mg/g of vitamin C, and 1.5 mg/g of pantothenic acid; the minerals include 113 mg/g of calcium carbonate, 39 mg/g of magnesium gluconate, 0.78 mg/g of manganese sulfate, 3 mg/g of ferrous lactate, 1.5 mg/g of zinc gluconate, 14 μg/g of sodium selenite, and 0.16 mg/g of copper sulfate; and the prebiotics content was 0.65 g/100 mL;
Step (4), the solidified probiotics was mixed (350 rpm, 30 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:2 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed evenly with an aqueous solution of an enteric coating material having a concentration of 8% according to a mass ratio of 1:6, washed for 3 times and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (45 rpm, 15 min) evenly with the wet hydrogel particles according to a mass ratio of 1:15 to obtain a mixture; the mixture was then pre-cooled at −80° C. for 2.5 h, and then freeze-dried at −55° C. for 36 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with four holes, each having an aperture of 1 mm, in equidistant symmetrical positions at both ends and the waist of the entire shell with laser (as shown in
Step (1), sodium carboxymethyl cellulose (a viscosity of 15000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 310:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:16) to obtain a mixture; the mixture was first stirred at 70 rpm for 100 min, and then stirred at 20 rpm for 14 h to obtain a gel; the gel was dried in a drying oven at 45° C. for 24 h, turned over and continued to dry for 32 h, then cross-linked at high temperature of 130° ° C. for 4.4 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed twice with distilled water, 2 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:100 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 3%, and repeatedly activated for 5 generations under the same culture conditions (38° C., 27 h); bacterial sludge was collected by low-temperature centrifugation (5° C., 5500 rpm, 25 min), and washed twice with 0.9% sterile normal saline; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 20% according to a volume ratio of 1:3 to obtain a bacterial suspension; the bacterial suspension was then mixed evenly with an aqueous solution of a natural polymer material having a concentration of 1.5% according to a volume ratio of 1:1 to obtain a mixed solution; the obtained mixed solution was then solidified with a 0.1 mol/L CaCl2) solution for 40 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a low temperature of 5° C. for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (150 rpm, 30 min) evenly with the aqueous solution of the natural polymer material having a concentration of 4% according to a mass ratio of 1:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 111 μg/g of vitamin A, 4 μg/g of vitamin D3, 4 mg/g of vitamin E, 8 μg/g of vitamin K2, 0.4 mg/g of vitamin B1, 0.4 mg/g of vitamin B2, 0.4 mg/g of vitamin B6, 0.4 μg/g of vitamin B12, 4 mg/g of niacinamide, 60 μg/g of folic acid, 25 mg/g of vitamin C, and 1.5 mg/g of pantothenic acid; the minerals include 113 mg/g of calcium carbonate, 39 mg/g of magnesium gluconate, 0.78 mg/g of manganese sulfate, 3 mg/g of ferrous lactate, 1.5 mg/g of zinc gluconate, 14 μg/g of sodium selenite, and 0.16 mg/g of copper sulfate; and the prebiotics content was 0.65 g/100 mL;
Step (4), the solidified probiotics was mixed (200 rpm, 20 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:3 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed with an aqueous solution of an enteric coating material having a concentration of 8% according to a mass ratio of 1:6, washed for 3 times and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (60 rpm, 20 min) evenly with the wet hydrogel particles according to a mass ratio of 1:17 to obtain a mixture; the mixture was then pre-cooled at −80° ° C. for 4 h, and then freeze-dried at −55° C. for 48 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with two holes, each having an aperture of 1 mm, in equidistant positions of skew symmetry from the vertex and midline of the entire capsule with laser (as shown in
Step (1), sodium carboxymethyl cellulose (a viscosity of 7000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 330:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:22) to obtain a mixture; the mixture was first stirred at 50 rpm for 80 min, and then stirred at 30 rpm for 16 h to obtain a gel; the gel was dried in a drying oven at 60° C. for 28 h, turned over and continued to dry for 36 h, then cross-linked at high temperature of 110° ° C. for 3.6 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed with distilled water for 4 times, 3 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:150 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 4.5%, and repeatedly activated for 5 generations under the same culture conditions (35° C., 21 h); bacterial sludge was collected by low-temperature centrifugation (4° C., 4500 rpm, 15 min), and washed with 0.95% sterile normal saline for 3 times; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 6% according to a volume ratio of 1:5 to obtain a bacterial suspension; the bacterial suspension was then mixed evenly with an aqueous solution of a natural polymer material having a concentration of 1% according to a volume ratio of 1:1 to obtain a mixed solution; the obtained mixed solution was then solidified with a 0.1 mol/L CaCl2) solution for 20 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a low temperature of 3ºC for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (350 rpm, 30 min) evenly with the aqueous solution of the natural polymer material having a concentration of 4% according to a mass ratio of 2:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 128 μg/g of vitamin A, 6 μg/g of vitamin D3, 6 mg/g of vitamin E, 10 μg/g of vitamin K2, 0.6 mg/g of vitamin B1, 0.6 mg/g of vitamin B2, 0.6 mg/g of vitamin B6, 0.7 μg/g of vitamin B12, 7 mg/g of niacinamide, 80 μg/g of folic acid, 40 mg/g of vitamin C, and 2.5 mg/g of pantothenic acid; the minerals include 133 mg/g of calcium carbonate, 51 mg/g of magnesium gluconate, 0.98 mg/g of manganese sulfate, 5 mg/g of ferrous lactate, 2.5 mg/g of zinc gluconate, 17 μg/g of sodium selenite, and 0.3 mg/g of copper sulfate; and the prebiotics content was 1.0 g/100 mL;
Step (4), the solidified probiotics was mixed (300 rpm, 30 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:4 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed evenly with an aqueous solution of an enteric coating material having a concentration of 8% according to a mass ratio of 1:4, washed for 4 times and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (30 rpm, 10 min) evenly with the wet hydrogel particles according to a mass ratio of 1:13 to obtain a mixture; the mixture was then pre-cooled at −80° ° C. for 1 h, and then freeze-dried at −55° C. for 18 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with two holes, each having an aperture of 1 mm, in symmetrical positions in the middle of the waist with laser to obtain a porous capsule shell; and the porous capsule shell was then combined with the capsule content to obtain the diet-reducing capsule containing multi-nutrient microspheres.
Step (1), sodium carboxymethyl cellulose (a viscosity of 11000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 310:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:22) to obtain a mixture; the mixture was first stirred at 60 rpm for 90 min, and then stirred at 20 rpm for 14 h to obtain a gel; the gel was dried in a drying oven at 60° C. for 28 h, turned over and continued to dry for 36 h, then cross-linked at high temperature of 120° C. for 4 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed twice with distilled water, 2 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:100 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 4.5%, and repeatedly activated for 5 generations under the same culture conditions (36.5° C., 24 h); bacterial sludge was collected by low-temperature centrifugation (3° C., 3500 rpm, 10 min), and washed twice with 0.95% sterile normal saline; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 6% according to a volume ratio of 1:5 to obtain a bacterial suspension; the bacterial suspension was then mixed evenly with an aqueous solution of a natural polymer material having a concentration of 1.5% according to a volume ratio of 1:1.5 to obtain a mixed solution; the obtained mixed solution was then solidified with a 0.1 mol/L CaCl2) solution for 30 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a low temperature of 4° C. for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (350 rpm, 30 min) evenly with the aqueous solution of the natural polymer material having a concentration of 4% according to a mass ratio of 1:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 128 μg/g of vitamin A, 6 μg/g of vitamin D3, 6 mg/g of vitamin E, 10 μg/g of vitamin K2, 0.6 mg/g of vitamin B1, 0.6 mg/g of vitamin B2, 0.6 mg/g of vitamin B6, 0.7 μg/g of vitamin B12, 7 mg/g of niacinamide, 80 μg/g of folic acid, 40 mg/g of vitamin C, and 2.5 mg/g of pantothenic acid; the minerals include 133 mg/g of calcium carbonate, 51 mg/g of magnesium gluconate, 0.98 mg/g of manganese sulfate, 5 mg/g of ferrous lactate, 2.5 mg/g of zinc gluconate, 17 μg/g of sodium selenite, and 0.3 mg/g of copper sulfate; and the prebiotics content was 1.0 g/100 mL;
Step (4), the solidified probiotics was mixed (300 rpm, 30 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:4 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed evenly with an aqueous solution of an enteric coating material having a concentration of 8% according to a mass ratio of 1:5, washed twice and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (45 rpm, 15 min) evenly with the wet hydrogel particles according to a mass ratio of 1:13 to obtain a mixture; the mixture was then pre-cooled at −80° C. for 2.5 h, and then freeze-dried at −55° C. for 36 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with two holes, each having an aperture of 0.5 mm, in equidistant symmetrical positions in the middle of the waist with laser to obtain a porous capsule shell; and the porous capsule shell was then combined with the capsule content to obtain the diet-reducing capsule containing multi-nutrient microspheres.
Step (1), sodium carboxymethyl cellulose (a viscosity of 15000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 330:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:16) to obtain a mixture; the mixture was first stirred at 50 rpm for 80 min, and then stirred at 30 rpm for 16 h to obtain a gel; the gel was dried in a drying oven at 45° C. for 28 h, turned over and continued to dry for 36 h, then cross-linked at high temperature of 120° C. for 4 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed with distilled water for 4 times, 3 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:200 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 3%, and repeatedly activated for 5 generations under the same culture conditions (38° C., 21 h); bacterial sludge was collected by low-temperature centrifugation (4° C., 3500 rpm, 20 min), and washed with 0.9% sterile normal saline for 3 times; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent to obtain a bacterial suspension; the bacterial suspension was then mixed evenly with an aqueous solution of a natural polymer material having a concentration of 1% according to a volume ratio of 1:1.5 to obtain a mixed solution; the obtained mixed solution was then solidified with a 0.1 mol/L CaCl2) solution for 30 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a lower temperature of 4° C. for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (150 rpm, 30 min) evenly with the aqueous solution of the natural polymer material having a concentration of 2% according to a mass ratio of 2:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 95 μg/g of vitamin A, 1 μg/g of vitamin D3, 1 mg/g of vitamin E, 6 μg/g of vitamin K2, 0.1 mg/g of vitamin B1, 0.1 mg/g of vitamin B2, 0.1 mg/g of vitamin B6, 0.1 μg/g of vitamin B12, 1 mg/g of niacinamide, 40 μg/g of folic acid, 10 mg/g of vitamin C, and 0.5 mg/g of pantothenic acid; 93 mg/g of calcium carbonate, 27 mg/g of magnesium gluconate, 0.58 mg/g of manganese sulfate, 1 mg/g of ferrous lactate, 0.1 mg/g of zinc gluconate, 10 μg/g of sodium selenite, and 0.01 mg/g of copper sulfate; and the prebiotics content was 0.3 g/100 mL;
Step (4), the solidified probiotics was mixed (100 rpm, 30 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:3 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed with an aqueous solution of an enteric coating material having a concentration of 8% according to a mass ratio of 1:6, washed for 3 times and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (45 rpm, 10 min) evenly with the wet hydrogel particles according to a mass ratio of 1:13 to obtain a mixture; the mixture was then pre-cooled at −80° C. for 1 h, and then freeze-dried at −55° C. for 48 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with two holes, each having an aperture of 0.75 mm, in symmetrical positions in the middle of the waist with laser to obtain a porous capsule shell; and the porous capsule shell was then combined with the capsule content to obtain the diet-reducing capsule containing multi-nutrient microspheres.
Step (1), sodium carboxymethyl cellulose (a viscosity of 7000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 350:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:22) to obtain a mixture; the mixture was first stirred at 60 rpm for 90 min, and then stirred at 30 rpm for 16 h to obtain a gel; the gel was dried in a drying oven at 45° C. for 28 h, turned over and continued to dry for 36 h, then cross-linked at high temperature of 110° ° C. for 4 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed with distilled water for 4 times, 2 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:200 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 1.5%, and repeatedly activated for 5 generations under the same culture conditions (37° C., 21 h); bacterial sludge was collected by low-temperature centrifugation (5° C., 5500 rpm, 15 min), and washed with 0.85% sterile normal saline for 3 times; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 13% according to a volume ratio of 1:5 to obtain a bacterial suspension; the bacterial suspension was then mixed evenly with an aqueous solution of a natural polymer material having a concentration of 1.5% according to a volume ratio of 1:0.5 to obtain a mixed solution; the obtained mixed solution was then solidified with a 0.1 mol/L CaCl2) solution for 30 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a low temperature of 3ºC for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (350 rpm, 10 min) evenly with the aqueous solution of the natural polymer material having a concentration of 4% according to a mass ratio of 1.5:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 95 μg/g of vitamin A, 1 μg/g of vitamin D3, 1 mg/g of vitamin E, 6 μg/g of vitamin K2, 0.1 mg/g of vitamin B1, 0.1 mg/g of vitamin B2, 0.1 mg/g of vitamin B6, 0.1 μg/g of vitamin B12, 1 mg/g of niacinamide, 40 μg/g of folic acid, 10 mg/g of vitamin C, and 0.5 mg/g of pantothenic acid; the minerals include 93 mg/g of calcium carbonate, 27 mg/g of magnesium gluconate, 0.58 mg/g of manganese sulfate, 1 mg/g of ferrous lactate, 0.1 mg/g of zinc gluconate, 10 μg/g of sodium selenite, and 0.01 mg/g of copper sulfate; and the prebiotics content was 0.3 g/100 mL;
Step (4), the solidified probiotics was mixed (300 rpm, 10 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:4 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed with an aqueous solution of an enteric coating material having a concentration of 8% according to a mass ratio of 1:6, washed for 3 times and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (60 rpm, 10 min) evenly with the wet hydrogel particles according to a mass ratio of 1:17 to obtain a mixture; the mixture was then pre-cooled at −80° ° C. for 2.5 h, and then freeze-dried at −55° C. for 48 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with two holes, each having an aperture of 1 mm, in symmetrical positions in the middle of the waist with laser to obtain a porous capsule shell; and the porous capsule shell was then combined with the capsule content to obtain the diet-reducing capsule containing multi-nutrient microspheres.
Step (1), sodium carboxymethyl cellulose (a viscosity of 11000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 330:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:16) to obtain a mixture; the mixture was first stirred at 60 rpm for 90 min, and then stirred at 30 rpm for 16 h to obtain a gel; the gel was dried in a drying oven at 45° C. for 24 h, turned over and continued to dry for 32 h, then cross-linked at high temperature of 120° C. for 4 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed with distilled water for 4 times, 3 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:150 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 3%, and repeatedly activated for 5 generations under the same culture conditions (37° C., 24 h); bacterial sludge was collected by low-temperature centrifugation (4° C., 4500 rpm, 15 min), and washed twice with 0.9% sterile normal saline; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 13% according to a volume ratio of 1:4 to obtain a bacterial suspension; the bacterial suspension was then solidified with a 0.1 mol/L CaCl2) solution for 30 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a low temperature of 4° ° C. for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (150 rpm, 20 min) evenly with the aqueous solution of the natural polymer material having a concentration of 3% according to a mass of 1.5:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 111 μg/g of vitamin A, 4 μg/g of vitamin D3, 4 mg/g of vitamin E, 8 μg/g of vitamin K2, 0.4 mg/g of vitamin B1, 0.4 mg/g of vitamin B2, 0.4 mg/g of vitamin B6, 0.4 μg/g of vitamin B12, 4 mg/g of niacinamide, 60 μg/g of folic acid, 25 mg/g of vitamin C, and 1.5 mg/g of pantothenic acid; the minerals include 113 mg/g of calcium carbonate, 39 mg/g of magnesium gluconate, 0.78 mg/g of manganese sulfate, 3 mg/g of ferrous lactate, 1.5 mg/g of zinc gluconate, 14 μg/g of sodium selenite, and 0.16 mg/g of copper sulfate; and the prebiotics content was 0.65 g/100 mL;
Step (4), the solidified probiotics was mixed (200 rpm, 20 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:3 to obtain a mixed nutrient solution;
Step (5), the mixed nutrient solution was mixed (45 rpm, 15 min) evenly with the wet hydrogel particles according to a mass ratio of 1:15 to obtain a mixture; the mixture was then pre-cooled at −80° ° C. for 2.5 h, and then freeze-dried at −55° C. for 36 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with four holes, each having an aperture of 1 mm, in the middle of the waist with laser to obtain a porous capsule shell; and the porous capsule shell was then combined with the capsule content to obtain the diet-reducing capsule containing multi-nutrient microspheres.
Step (1), sodium carboxymethyl cellulose (a viscosity of 11000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 330:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:16) to obtain a mixture; the mixture was stirred at 60 rpm for 90 min, and then stirred at 30 rpm for 16 h to obtain a gel; the gel was dried in a drying oven at 45° C. for 24 h, turned over and continued to dry for 32 h, then cross-linked at high temperature of 120° C. for 4 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed with distilled water for 4 times, 3 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:150 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 3%, and repeatedly activated for 5 generations under the same culture conditions (37° C., 24 h); bacterial sludge was collected by low-temperature centrifugation (4° C., 4500 rpm, 15 min), and washed twice with 0.9% sterile normal saline; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 13% according to a volume ratio of 1:4 to obtain a bacterial suspension; the bacterial suspension was then solidified with a 0.1 mol/L CaCl2) solution for 30 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a low temperature of 4ºC for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder (the vitamins include vitamin A, vitamin D3, vitamin E, vitamin K2, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacinamide, folic acid, vitamin C, and pantothenic acid; the minerals include calcium carbonate, magnesium gluconate, manganese sulfate, ferrous lactate, zinc gluconate, sodium selenite, and copper sulfate; and the contents of vitamins, minerals and prebiotics in the finished diet-reducing capsule containing multi-nutrient microspheres were the same as those in Example 1);
Step (4), the vitamin-mineral-prebiotics composite powder, the bacterial suspension and the wet hydrogel particles were mixed (45 rpm, 15 min) evenly according to a mass ratio of 0.5:0.5:15 to obtain a mixture; the mixture was then pre-cooled at −80° C. for 2.5 h, and then freeze-dried at −55° C. for 36 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (5), an empty capsule shell was fixed and punched with two holes, each having an aperture of 1 mm, in the middle of the waist with laser to obtain a porous capsule shell; and the porous capsule shell was then combined with the capsule content to obtain the diet-reducing capsule containing multi-nutrient microspheres.
Step (1), sodium carboxymethyl cellulose (a viscosity of 3000) was added to an aqueous solution containing citric acid (a mass ratio of sodium carboxymethyl cellulose to citric acid was 380:1, and a mass ratio of sodium carboxymethyl cellulose to distilled water was 1:32) to obtain a mixture; the mixture was first stirred at 100 rpm for 150 min, and then stirred at 60 rpm for 30 h to obtain a gel; the gel was dried in a drying oven at 75° C. for 30 h, turned over and continued to dry for 40 h, then cross-linked at high temperature of 130° C. for 6 h, crushed and sieved to obtain solid hydrogel particles; the solid hydrogel particles were washed with distilled water for 8 times, 4 h each time (a mass ratio of the solid hydrogel particles to the distilled water was 1:150 in each washing), and filtered to obtain wet hydrogel particles;
Step (2), probiotics was inoculated in a sterile MRS liquid medium according to an inoculation amount of 7%, and repeatedly activated for 5 generations under the same culture conditions (39° C., 29 h); bacterial sludge was collected by low-temperature centrifugation (6° ° C., 6600 rpm, 30 min), and washed with 0.95% sterile normal saline for 4 times; the washed bacterial sludge was then mixed evenly with an aqueous solution of a freeze-drying protective agent having a concentration of 25% according to a volume ratio of 1:2 to obtain a bacterial suspension; the bacterial suspension was then solidified with a 0.1 mol/L CaCl2) solution for 50 min, washed and filtered to obtain solidified probiotics; and the solidified probiotics was stored at a low temperature of 6° ° C. for later use;
Step (3), powders of vitamins, minerals and prebiotics were mixed evenly, and sieved with a 70-mesh sieve to obtain vitamin-mineral-prebiotics composite powder; and the vitamin-mineral-prebiotics composite powder was then mixed (200 rpm, 30 min) evenly with the aqueous solution of the natural polymer material having a concentration of 6% according to a mass ratio of 3:11 to obtain a vitamin-mineral-prebiotics-natural polymer material mixed solution, wherein in the mixed solution, the contents of the vitamins, the minerals and the prebiotics were: 111 μg/g of vitamin A, 4 μg/g of vitamin D3, 4 mg/g of vitamin E, 8 μg/g of vitamin K2, 0.4 mg/g of vitamin B1, 0.4 mg/g of vitamin B2, 0.4 mg/g of vitamin B6, 0.4 μg/g of vitamin B12, 4 mg/g of niacinamide, 60 μg/g of folic acid, 25 mg/g of vitamin C, and 1.5 mg/g of pantothenic acid; the minerals include 113 mg/g of calcium carbonate, 39 mg/g of magnesium gluconate, 0.78 mg/g of manganese sulfate, 3 mg/g of ferrous lactate, 1.5 mg/g of zinc gluconate, 14 μg/g of sodium selenite, and 0.16 mg/g of copper sulfate; and the prebiotics content was 0.65 g/100 mL;
Step (4), the solidified probiotics was mixed (400 rpm, 40 min) evenly with the vitamin-mineral-prebiotics-natural polymer material mixed solution according to a mass ratio of 1:6 to obtain a mixed nutrient solution, and the mixed nutrient solution was then mixed with an aqueous solution of an enteric coating material having a concentration of 2% according to a mass ratio of 1:8, washed for 5 times and filtered to obtain wet probiotics-prebiotics-vitamin-mineral microspheres;
Step (5), the wet probiotics-prebiotics-vitamin-mineral microspheres were mixed (60 rpm, 40 min) evenly with the wet hydrogel particles according to a mass ratio of 1:20 to obtain a mixture; the mixture was then pre-cooled at −80° C. for 5 h, and then freeze-dried at −55° C. for 60 h under a vacuum degree of 25 Pa to obtain a freeze-dried product; the freeze-dried product was then crushed and sieved to obtain a capsule content; and
Step (6), an empty capsule shell was fixed and punched with four holes, each having an aperture of 1 mm, in the middle of the waist with laser to obtain a porous capsule shell; and the porous capsule shell was then combined with the capsule content to obtain the diet-reducing capsule containing multi-nutrient microspheres.
The Performances of the Diet-Reducing Capsule Containing Multi-Nutrient Microspheres of the Present Disclosure were Measured.
The samples were placed in simulated gastric juice for 2 h respectively, and then the microspheres were separated, washed and then stored at 4° C. for later use. 1 g of microspheres were added to 9 mL of phosphate buffer solution, then placed in a 37° C. shaker and shaken at 230 rpm for 30 min, followed by sampling, and the total number of viable bacteria was calculated.
The total number of viable bacteria in probiotics was determined by plate counting, and the specific operation was as follows: activated strains were mixed evenly in an aseptic operation cabinet to obtain a mixture; under the conditions of aseptic operation, the mixture was sequentially diluted into different gradients with sterile water in 10-fold increments; and the diluted products of three suitable dilution gradients were taken respectively, evenly inoculated in an MRS agar solid medium respectively, and invertedly cultured in a constant-temperature incubator at 37° C. for 24 h. Flat plates with the number of colonies between 30 to 300 were counted, and the total number of colonies was calculated according to a formula: total number of colonies (CFU/g)=average number of colonies of the same dilution gradient×dilution factor×5. The test results were shown in Table 1.
The samples were placed in simulated gastric juice for 2 h, and then microspheres were separated, washed and then stored at 4° C. for later use.
1 g of microspheres were added to 9 mL of phosphate buffer solution, shaken in a shaker at 37° C., 230 rpm for 30 min, followed by sampling; the number of viable bacteria was calculated, and the vitamin content, mineral content and prebiotics content were determined. The embedding rate was calculated according to the following formula:
The test results were shown in Table 2.
The samples were placed at normal temperature and pressure (indoor), and the contents of vitamins, minerals, probiotics and prebiotics in the samples were determined every 2 weeks, lasting for 10 weeks. The determination method of fat-soluble vitamin content refers to the “BJS 201717 Determination of 9 Fat-soluble Vitamins in Health Food”. The determination method of water-soluble vitamin content refers to the “BJS 201716 Determination of 9 Water-soluble Vitamins in Health Food”. The determination method of mineral content refers to the “BJS 201718 Determination of 9 Mineral Elements in Health Food”. The determination method of probiotics content is the same as that in Test (I). The detection method of fructooligosaccharides (prebiotics) refers to “GB/T 23528.2-2021 Quality Requirements for oligosaccharides Part 2: Fructooligosaccharides”. The test results were shown in Tables 3, 4, 5 and 6.
A certain mass of samples was weighed (denoted as M0) and placed in 100 mL of pre-prepared 37° C. diluted artificial gastric juice respectively (a volume ratio of the artificial gastric juice to sterile distilled water was 1:8, pH 2.10), and gently stirred for 0.5 h at a rotation speed of 45 rpm, followed by timing (no bubble was allowed). After the timing was over, excess diluted artificial gastric juice was removed with a stainless steel filter, absorbent paper was used to wipe off the moisture on the surface, and the samples were weighed and counted (denoted as M1). The MUR was calculated according to a formula. The test was repeated for three samples in parallel, and an average was taken from the results. Formula: MUR=(M1−M0)/M0. The test results were shown in
The elasticity of a sample was evaluated by dynamic mechanical analysis (DMA). After the swelling rate determination was over, the sample was immediately placed between parallel plates (with crosshatches and a diameter of 40 mm) of a rotational rheometer, and the elastic modulus was determined. A gap between the steel plates was set to 4 mm. The value of the elastic modulus at a frequency of 10 rad/s was used to determine the elasticity of sample particles. The test was repeated for three samples in parallel, and an average was taken from the results. The test results were shown in
The samples used in this test were prepared with reference to the method of Example 1, except that the concentration of the enteric coating material was changed according to
The samples (different concentrations of enteric coating material) were placed in sterile water for 2 h, and then microspheres were separated, washed and then stored at 4° C. for later use. 1 g of microspheres were added to 9 mL of phosphate buffer solution, then placed in a 37° C. shaker and shaken at 230 rpm for 30 min, followed by sampling, and the total number of viable bacteria was calculated.
The determination method for the total number of viable bacteria in probiotics was the same as that in Test (I). The test results were shown in
The samples used in this test were the samples prepared in Example 1.
The samples were placed in sterile water for 2 h, and then the microspheres were separated, washed and stored at 4° C. for later use. 1 g of microspheres were added to 9 containers each containing 9 mL of artificial intestinal fluid. The containers were then placed in a 37° C. shaker and shaken at 230 rpm for different times (0 min, 15 min, 30 min, 45 min, 60 min, 75 min, 90 min, 105 min, and 120 min), followed by sampling. The total number of viable bacteria was calculated.
The determination method for the total number of viable bacteria in probiotics was the same as that in Test (I). The test results were shown in
The samples used in this test were prepared with reference to the method of Example 1, except that the empty capsule shell was punched with different laser punching methods, and other conditions were the same as those of Example 1.
A test design for the effects of different laser punching methods on determination of the disintegration time of a capsule was shown in Table 7.
Capsule disintegration time determination method: the test was carried out in accordance with the “Pharmacopoeia of the People's Republic of China”, Edition 2020, Part IV, General Principles 0921, “Disintegration Time Limit Inspection Method”. Six test sample capsules were taken, a disintegration time limit tester was checked and equipped with a baffle, the temperature was set to 37° ° C., and the time when the capsules were completely dissolved in the artificial gastric juice was recorded. The capsules should be completely disintegrated within 30 min. If there was one capsule that cannot be completely disintegrated, another six capsules should be taken for retesting. If there was another one capsule that cannot be completely disintegrate, it would be recorded as unqualified. The test was repeated for three samples in parallel, and an average was taken from the results. The test results were shown in
Example 1 was selected as a test sample. Five male and female obese rats of the same age and sexual maturity were taken respectively, with a male rat weight of 500 to 520 g and a female rat weight of 400 to 420 g. The obese rats were given one capsule 30 min before meals every day at regular time, the obese rats were given normal diet after 30 min, and the test continued in this way for 63 days; and the weights of the obese rats were weighed and recorded at regular time of 20:00 every 7 days. The test results were shown in
As can be seen from Table 1, compared with Comparative examples 1 to 3, the number of viable bacteria in each of Examples 1 to 9 was more than 108, indicating that the probiotics in the microspheres had good acid resistance. As can be seen from Table 2, in Examples 1 to 9, the embedding rates of probiotics and vitamins were more than 80% respectively, the embedding rate of minerals was more than 70%, the embedding rate of prebiotics was more than 75%, and the embedding rate of nutrients in the microspheres was good. As can be seen from Tables 3, 4, 5 and 6, the probiotics, vitamins, minerals and prebiotics had good storage stability. As can be seen from
Although the contents of the present disclosure have been described in detail by the preferred examples above, it should be recognized that the above description should not be considered as a limitation on the present disclosure. After a person skilled in the art has read the above contents, a variety of modifications and substitutions made for the present disclosure will be obvious. Therefore, the protection scope of the present disclosure should be defined by the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202310044315.5 | Jan 2023 | CN | national |
