Claims
- 1. A process for the preparation of a 3-oxo-.DELTA..sup.1,4 -steroid of the formula ##STR6## wherein X is hydrogen, chlorine, fluorine or methyl, ##STR7## wherein Y is hydrogen, fluorine, or chlorine, and ##STR8## wherein Z is hydrogen, hydroxy, fluorine, or chlorine,
- R.sub.1 is hydrogen or an alicyclic hydrocarbon residue of 1-4 carbon atoms,
- R.sub.2 is hydrogen, hydroxy, alkyl of 1-6 carbon atoms optionally interrupted by an oxygen atom or a sulfur atom, alkanoyloxy of 1-6 carbon atoms, or benzoyloxy,
- R.sub.3 is hydrogen, hydroxy or methyl, and
- R.sub.4 and R.sub.5 each independently is alkyl or 1-4 carbon atoms, comprising
- fermenting a corresponding 3-oxo-.DELTA..sup.4 -steroid of the formula ##STR9## wherein X, ##STR10## are as defined above, or a 21-ester thereof with an alkanecarboxylic acid of 1-6 carbon atoms, with a living culture of Arthrobacter simplex in the presence of 0.04 g to 0.12 g of cobalt(II) ions per liter of culture broth.
- 2. A process of claim 1 wherein the Co ions are provided by adding CoCl.sub.2, Co(NO.sub.3).sub.2, CoSO.sub.4 or CoSO.sub.4.7H.sub.2 O to the culture broth.
- 3. A process of claim 1 wherein the concentration of cobalt(II) ions is 0.04-0.08 g per liter of broth.
- 4. A process of claim 1 wherein the Co ions are provided by adding CoSO.sub.4.7H.sub.2 O to the culture broth.
- 5. A process of claim 1, wherein the living culture of Arthrobacter simplex is of the strain ATCC 6946.
- 6. A process of claim 1, wherein the living culture of Arthrobacter simplex is of the strain IFO (3530) or ATCC 13 260.
Priority Claims (1)
Number |
Date |
Country |
Kind |
3049399 |
Dec 1980 |
DEX |
|
Parent Case Info
This is a continuation of application Ser. No. 561,510 filed Dec. 15, 1983 now abandoned, which is a continuation of Ser. No. 334,045, filed Dec. 23, 1981, now abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3388042 |
Arima et al. |
Jun 1968 |
|
4101378 |
Nishikawa et al. |
Jul 1978 |
|
4431732 |
Weber et al. |
Feb 1984 |
|
Continuations (2)
|
Number |
Date |
Country |
Parent |
561510 |
Dec 1983 |
|
Parent |
334045 |
Dec 1981 |
|