Claims
- 1. Process for the production of a solid phase matrix of an insoluble carrier with a specifically bindable substance bound thereto, comprising:
- (i) coating a substance on an insoluble carrier material, wherein said substance contains a first polymer, said first polymer is a protein polymer comprising hydrophobic protein molecules having a molecular weight of from 10,000 to 700,000 which have been cross-linked with a bifunctional or polyfunctional compound to produce said protein polymer, and a plurality of molecules of a first member of a specific binding pair P1, and
- (ii) cross linking said first polymer to a second polymer which comprises a plurality of molecules of a second member of a specific binding pair P2, and binding sites for a complex of an immune component and an analyte, wherein said substance and said second polymer are cross linked via binding of P1 and P2 to each other, wherein said first polymer is more hydrophobic than said second polymer.
- 2. Process of claim 1, wherein said protein is a soluble protein having a molecular weight above about 200,000 and is more hydrophobic than both P.sub.1 and P.sub.2.
- 3. Process of claim 1, wherein said protein polymer has a molecular weight of from 200,000 to 20,000,000.
- 4. Process of claim 1, wherein said bifunctional compound is disuccinimidyl suberate.
- 5. Process of claim 1, wherein said protein is bovine serum albumin or immune-gamma globulin.
- 6. Process of claim 1, wherein said second polymer consists of cross-linked molecules of P.sub.2 and a homo-, heterobi- or polyfunctional linker.
- 7. Process of claim 1, wherein said second polymer consists of molecules of P.sub.2.
- 8. Process of claim 1, wherein said second polymer comprises a second component cross-linked with P.sub.2.
- 9. Process of claim 1, wherein said second polymer comprises a hydrophobic protein.
- 10. Process of claim 1, wherein said second polymer comprises a hydrophobic protein cross-linked to P.sub.2.
- 11. Process of claim 1, wherein the specific binding pair of P.sub.1 -P.sub.2 is selected from the group consisting of biotin-avidin, biotin-streptavidin, antigen-antibody, hapten-antibody, protein A-immune-.gamma.-globulin.
- 12. Process according to claim 1, wherein said insoluble solid carrier is furan, glass, titanium, dioxide, polystyrene, .gamma.-globulin activated polystyrene, paper, or polyolefin.
- 13. Solid matrix useful in determining an analyte in a sample prepared by the process of claim 1.
Priority Claims (1)
Number |
Date |
Country |
Kind |
3806431 |
Feb 1988 |
DEX |
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Parent Case Info
This application is a continuation of application Ser. No. 435,427, filed Oct. 30, 1989, now abandoned.
US Referenced Citations (4)
Foreign Referenced Citations (5)
Number |
Date |
Country |
16552 |
Oct 1980 |
EPX |
122209 |
Oct 1984 |
EPX |
269092 |
Jun 1988 |
EPX |
8704794 |
Aug 1987 |
WOX |
2103791 |
Feb 1983 |
GBX |
Continuations (1)
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Number |
Date |
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Parent |
435427 |
Oct 1989 |
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