Claims
- 1. A method for preparing a non-thrombogenic apyrase-carrying prosthetic plastic material comprising the steps of hydrolytically activating the surface of a plastic material carrier selected from the group consisting of a polyamide polymer and a polyethylene terephthalate polymer, treating the hydrolyzed plastic carrier by immersing it into a buffered solution of a cross-linking agent selected from the group consisting of glutaraldehyde and N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide and in a buffered solution of apyrase, and allowing said immersed plastic material carrier to stand for a time sufficient to effect the covalent bonding of apyrase to the surface of said plastic material carrier.
- 2. The method of claim 1 wherein said plastic material carrier is a polyamide and said hydrolysis is an acid hydrolysis.
- 3. The method of claim 1 wherein said plastic material carrier is polyethylene terephthalate, said hydrolysis is an alkaline hydrolysis, and said solution of a cross-linking agent is a buffered solution of N-ethyl-N'-(dimethylaminopropyl)carbodiimide, which solution is combined with said buffered solution of apyrase prior to the immersion therein of said hydrolytically-activated plastic material carrier.
- 4. Non-thrombogenic apyrase-carrying prosthetic plastic material articles prepared by the method of claim 1 consisting of plastic material from the group consisting of polyamides and polyethylene terephthalate having said apyrase covalently and permanently bonded on the surface thereof.
Priority Claims (1)
Number |
Date |
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Kind |
24838 A/79 |
Aug 1979 |
ITX |
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Parent Case Info
The present application is a continuation of application Ser. No. 165,708 filed July 3, 1980, now abandoned.
US Referenced Citations (3)
Non-Patent Literature Citations (2)
Entry |
Patel et al., In Soluble Matrix-Supported Apyrase, Deoxyribonuclease and Cholinesterase Biochim. Biophys. Acta, vol. 178, 1969, (pp. 626-629). |
Gaarder et al., Adenosine Diphosphate in Red Cells as a Factor in the Adhesiveness of Human Blood Platelets, Nature, vol. 192, 1961, (pp. 531-532). |
Continuations (1)
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Number |
Date |
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Parent |
165708 |
Jul 1980 |
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