Preparative Electrophoresis Device for Proteomics

Information

  • Research Project
  • 6833665
  • ApplicationId
    6833665
  • Core Project Number
    R43EB003216
  • Full Project Number
    1R43EB003216-01A1
  • Serial Number
    3216
  • FOA Number
  • Sub Project Id
  • Project Start Date
    9/1/2004 - 19 years ago
  • Project End Date
    11/30/2005 - 18 years ago
  • Program Officer Name
    SWAIN, AMY L
  • Budget Start Date
    9/1/2004 - 19 years ago
  • Budget End Date
    11/30/2005 - 18 years ago
  • Fiscal Year
    2004
  • Support Year
    1
  • Suffix
    A1
  • Award Notice Date
    9/2/2004 - 19 years ago
Organizations

Preparative Electrophoresis Device for Proteomics

DESCRIPTION (provided by applicant): The overall goal of this research and development effort is the commercialization of a vortex-stabilized electrophoretic focusing device designed for preparative separation of biologics and other solutes of interest for proteomic-type investigations. A feasibility study will be conducted that includes 3D solids modeling of the device, fabrication, and experimental testing of the preparative device. The major aim is to demonstrate the ability to separate solutes, whether isoforms or otherwise, by various modes of electrophoresis using the same preparative device (i.e., IEF, ITP, or continuous flow electrophoresis), while reducing time required for device set-up, sample separation, and product recovery. The preparative device employs a precision engineered chamber that enhances separation performance allowing operation at high field strengths, without the detrimental effects of the associated joule heating. It is possible to operate at fields greater than 500 V/cm and at capacities of hundreds of milligrams per sample load. In addition, the chamber design, combined with performing separations in free solution, enables interfacing the device for convenient product collection and follow-on analysis. It is believed the device will enable researchers to isolate sufficient quantities of target molecules for full characterization studies in less time, with less manpower, thereby increasing efficiency of the discovery process. The overall commercial target for the vortex-stabilized device is to provide a high-resolution, high capacity turn-key suite for free solution separation of complex mixtures with application to proteomics.

IC Name
NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING
  • Activity
    R43
  • Administering IC
    EB
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    99889
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    286
  • Ed Inst. Type
  • Funding ICs
    NIA:99888\NIBIB:1\
  • Funding Mechanism
  • Study Section
    ZRG1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    PROTASIS CORPORATION
  • Organization Department
  • Organization DUNS
  • Organization City
    MARLBORO
  • Organization State
    MA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    017523032
  • Organization District
    UNITED STATES