Claims
- 1. A pair of oligonucleotide primers for a polymerase chain reaction, which primers are sufficiently complementary to conserved regions among the nucleic acid sequences of AIDS viruses to hybridize therewith and not sufficiently complementary to HTLVI nucleic acids to hybridize therewith, wherein said conserved regions are at least 20 nucleotides long, wherein said primer pair consists of a member that comprises a nucleic acid sequence at least 14 nucleotides in length, which nucleic acid sequence is contained within a sequence selected from the group of nucleic acid sequences consisting of:
- 5'-CAGGGAGCTAGAACGAT-3' and 5'-ACCTGCCACCTGTAGTAG-3',
- and a member that comprises a nucleic acid sequence at least 14 nucleotides in length, which nucleic acid sequence is contained within a sequence selected from the group of nucleic acid sequences consisting of:
- 5'-CTTCTGATCCTGTCTGA-3' and 5'-GCCATATTCCTGGACTACAG-3'.
- 2. An oligonucleotide primer comprising a nucleic acid sequence which sequence is sufficiently complementary to a conserved region among the nucleic acid sequences of AIDS viruses, and specific to the nucleic acids of AIDS viruses, to hybridize therewith and act as a point of initiation of synthesis in an amplification reaction, wherein said primer is selected from the group consisting of:
- 5'-CAGGGAGCTAGAACGAT-3', 5'-CTTCTGATCCTGTCTGA-3',
- 5'-ACCTGCCACCTGTAGTAG-3', and 5'-GCCATATTCCTGGACTACAG-3'.
- 3. A DNA probe for detecting or monitoring amplified AIDS virus nucleic acids in a sample capable of hybridizing to an AIDS virus nucleic acid sequence, wherein said probe consists of a nucleic acid sequence between 28 and 180 nucleotides in length, which probe nucleic acid sequence contains a subsequence at least 28 nucleotides in length of a sequence selected from the group of nucleic acid sequences consisting of:
- 5'-AATCCTGGCCTGTTAGAAACATCAGAAG-3',
- 5'-TAGTAGCCAGCTGTGATAAATGTCAGCTAAAAGGAGAAGCC-3', and the sequences fully complementary thereto, and wherein said probe, when hybridized to its complementary sequence, encompasses a restriction enzyme cleavage site.
- 4. A DNA probe according to claim 3 comprising a nucleic acid sequence, or a sequence fully complementary to, a sequence selected from the group consisting of:
- 5'-AATCCTGGCCTGTTAGAAACATCAGAAG-3' and
- 5'-TAGTAGCCAGCTGTGATAAATGTCAGCTAAAAGGAGAAGCC-3'.
- 5. A DNA probe for detecting amplified AIDS virus nucleic acids in a sample, wherein said probe is capable of hybridizing to an AIDS virus nucleic acid sequence, and wherein said probe is selected from the group consisting of:
- 5'-AATCCTGGCCTGTTAGAAACATCAGAAG-3' and
- 5'-TAGTAGCCAGCTGTGATAAATGTCAGCTAAAAGGAGAAGCC-3'.
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation, of application Ser. No. 08/092,767, filed Jul. 16, 1993, now U.S. Pat. No. 5,356,022, which is a continuation of U.S. Ser. No. 07/918,907, filed Jul. 22, 1992, now abandoned, which is a continuation of U.S. Ser. No. 07/639,103, filed Jan. 9, 1991, now abandoned, which is a continuation of U.S. Ser. No. 07/394,276, filed Aug. 15, 1989, which issued as U.S. Pat. No. 5,008,182, which is a continuation of U.S. Ser. No. 935,581, filed Nov. 26, 1986, now abandoned, which is a continuation-in-part application of U.S. Ser. No. 818,127, filed Jan. 10, 1986, now abandoned. U.S. Ser. No. 07/394,276 is also a continuation-in-part application of U.S. Ser. No. 828,144, filed Feb. 7, 1986, which issued as U.S. Pat. No. 4,683,195 on Jul. 28, 1987, which is a continuation-in-part of U.S. Ser. No. 824,044, filed Jan. 30, 1986, now abandoned, which is a divisional of U.S. Ser. No. 791,308, filed Oct. 25, 1985, which issued as U.S. Pat. No. 4,683,202 on Jul. 28, 1987, and which is a continuation-in-part of U.S. Ser. No. 716,975, filed Mar. 28, 1985, now abandoned.
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Divisions (1)
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Continuation in Parts (4)
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