Claims
- 1. A probe carrier, characterized by comprising a single-stranded DNA probe immobilized to a carrier having a thin film containing (111)-oriented single crystal gold formed thereon through a sulfur atom.
- 2. A probe carrier according to claim 1, wherein surface unevennesses of the thin film containing (111)-oriented single crystal gold are 0.5 nm or less per μm2.
- 3. A probe carrier according to claim 1, wherein the sulfur atom interposed between the carrier and the single-stranded DNA probe is formed as a functional group of the single-stranded DNA probe.
- 4. A probe carrier according to claim 1, wherein the single-stranded DNA probe has a thiol group as a functional group.
- 5. A probe carrier according to claim 1, wherein an ink jet method is used to apply the single-stranded DNA probe to the carrier upon immobilizing the single-stranded DNA probe to the carrier.
- 6. A probe carrier according to claim 1, wherein a method in which the carrier is immersed in a gold complex solution to form a gold single crystal, thin film on the carrier is used as a method of forming the thin film containing the (111)-oriented single crystal gold.
- 7. A probe carrier according to claim 1, wherein the thin film containing the (111)-oriented single crystal gold is used as an electrode.
- 8. A probe carrier according to claim 1, wherein the thin film containing the (111)-oriented single crystal gold can be applied with a voltage.
- 9. A method of producing a probe carrier having a single-stranded DNA probe immobilized to a carrier having a thin film containing (111)-oriented single crystal gold formed thereon through a sulfur atom, the method comprising the steps of:
forming the thin film containing (111)-oriented single crystal gold on the carrier; and immobilizing the single-stranded DNA probe to the thin film through the sulfur atom.
- 10. A method of producing a probe carrier according to claim 9, wherein surface unevennesses of the thin film containing the (111)-oriented single crystal gold are 0.5 nm or less per μm2.
- 11. A method of producing a probe carrier according to claim 9, wherein the sulfur atom interposed between the carrier and the single-stranded DNA probe is formed as a functional group of the single-stranded DNA probe.
- 12. A method of producing a probe carrier according to claim 9, wherein an ink jet method is used to apply the single-stranded DNA probe to the carrier upon immobilizing the single-stranded DNA probe to the carrier.
- 13. A method of producing a probe carrier according to claim 9, wherein the method in which a carrier is immersed in a gold complex solution to form a gold single crystal thin film on the carrier is used as a method of forming the thin film containing the (111)-oriented single crystal gold.
- 14. A method of producing a probe carrier according to claim 9, wherein the thin film containing the (111)-oriented single crystal gold comprises a patterned gold single crystal film.
- 15. A method of producing a probe carrier according to claim 14, wherein an electron beam or ion is applied to the carrier for the patterning.
- 16. A method of evaluating a probe carrier, characterized by comprising observing and inspecting a form of the probe carrier produced by the method according to claim 9 through a scanning probe microscope.
- 17. A method of detecting a target nucleic acid using a probe carrier having a single-stranded DNA probe for the detection of the target nucleic acid, characterized in that the probe carrier is the probe carrier of any one of claims 1 to 8.
- 18. A method of detecting a target nucleic acid according to claim 17, wherein the thin film containing the (111)-oriented single crystal gold is used as an electrode and the target nucleic acid is detected by electrochemical measurement using the electrode.
- 19. A molecular device manufactured by using a thin film containing (111)-oriented single crystal gold as electrodes and interconnecting the electrodes by using a molecular chain typified by DNA.
Priority Claims (1)
Number |
Date |
Country |
Kind |
2002-191224 |
Jun 2002 |
JP |
|
Parent Case Info
[0001] This application is a continuation of International Application No. PCT/JP03/08196, filed Jun. 27, 2003, which claims the benefit of Japanese Patent Application No. 2002-191224, filed Jun. 28, 2002.
Continuations (1)
|
Number |
Date |
Country |
Parent |
PCT/JP03/08196 |
Jun 2003 |
US |
Child |
10725396 |
Dec 2003 |
US |