SEQUENCE LISTING
The application contains a Sequence Listing which has been submitted electronically in .XML format and is hereby incorporated by reference in its entirety. The sequence listing does not go beyond the disclosure of the PCT priority application as filed. Said .XML copy, is named “IP-2346-PCT_SL.xml” and is 31,630 kb in size. The sequence listing contained in this .XML file is part of the specification and is hereby incorporated by reference herein in its entirety.
DESCRIPTION
Field
This disclosure relates to probes for improving environmental sample (including wastewater samples and other samples) surveillance and surveillance of other samples for various coronaviruses. Libraries enriched with the present methods may be used to generate sequencing data. Also described are probes and methods for enzymatic depletion of unwanted RNA and cDNA from human wastewater and other samples.
Background
Viruses continue to develop naturally resulting in new strains and diseases to human populations. For example, the World Health Organization (WHO) declared infection by the novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-COV-2) as a pandemic and termed the related disease as coronavirus disease 2019 (COVID-19). Although a large percentage of persons infected with this novel virus experience mild to moderate respiratory, gastrointestinal, cardiovascular, and/or other discomforts without requiring medical care, infected persons with underlying medical problems and/or comorbidities, such as diabetes, cardiovascular disease, chronic respiratory disease, or cancer, are more likely to develop serious illness and/or die from COVID-19 or related secondary infections.
Transmission vectors of SARS-COV-2, and variants thereof, are under heavy investigation. Infected subjects, whether symptomatic or asymptomatic, shed virus and/or inactive viral particles thereof into community sewer systems through feces, nasal/sinus drainage/mucus, and phlegm. While this presents an opportunity to investigate wastewater for incidence of disease, sampling and measuring wastewater for a virus-of-interest such as SARS-COV-2 and/or variants thereof is problematic due to low concentrations of virus or particles thereof alone, or in combination with contaminants (e.g., other waterborne pathogens or human nucleic acids) in the wastewater. Non-limiting examples of waterborne pathogens include bacterial, viral, fungal, and parasitic pathogens, such as fecal coliforms. The mixture of contaminants and pathogens presents a difficult medium for viral DNA and RNA extraction therefrom, especially where concentrations of a virus-of-interest are low.
As such, public health officials need methods of enriching wastewater samples for coronavirus to quantify incidence of viral infection or disease in a community and to identify novel coronaviruses of interest in wastewater, such as from a sewer system. Public health officials also need methods of recovering nucleic acids from a virus-of-interest in wastewater. Investigations of other types of samples would also benefit from improved methods of recovering nucleic acids. Monitoring of other samples also provides valuable public health information and would benefit from improved methods of recovering nucleic acids.
Described herein is the development of a pan-coronavirus probe set for enrichment and detection of novel coronaviruses. Through an iterative design process, probes described herein are designed to have a broad diversity of targets in order to increase the odds of capturing genomic sequence from an as of yet undiscovered or novel variant coronavirus. The probe set described herein, simultaneously minimizes the overall number of oligonucleotides that are necessary to detect such a broad diversity of sequences and minimizes the amount of redundancy.
SUMMARY
In accordance with the description, described herein are methods of enriching a sample for one or more target coronavirus nucleic acids and/or for improving environmental wastewater surveillance for various coronaviruses. These methods may be performed with standard lab equipment, such as flowcells comprised in sequencers. In some embodiments, standard sequencing consumables and platform (i.e., sequencer) can be used as a microfluidic device for enriching and/or depleting library fragments. In some embodiments, depleting abundant small noncoding RNAis performed after cDNA synthesis and amplification.
Embodiment 1. A method of enriching a sample for one or more target viral nucleic acids comprising the steps of: (a) providing a probe set comprising at least two nucleic acid probes complementary to one or more target viral nucleic acids, wherein the probe set comprises at least two of SEQ ID NOs: 1-22909; (b) allowing the probes in the probe set to hybridize to the target viral nucleic acids; (c) enriching the sample for the one or more target viral nucleic acids by amplifying the target viral nucleic acids and/or separating the target viral nucleic acids from the sample.
Embodiment 2. A method of enriching a sample for one or more target coronavirus nucleic acids comprising the steps of: (a) providing a probe set comprising at least two nucleic acid probes complementary to one or more target coronavirus nucleic acids, wherein the nucleic acid probes are affixed to a support; (b) capturing the one or more target coronavirus nucleic acids on the support; (c) using the one or more captured target coronavirus nucleic acids as a template strand to produce one or more nucleic acid duplexes immobilized on the support, wherein the one or more target coronavirus nucleic acids hybridize to one or more probes of the probe set on the support; (d) contacting a transposase and transposon with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand; (e) contacting the one or more tagged nucleic acid duplexes with a nucleic acid modifying enzyme under conditions to extend the 3′ end of the immobilized strand to the 5′ end of the template strand to produce one or more end-extended tagged nucleic acid duplexes; (f) amplifying the one or more end-extended tagged nucleic acid duplexes to produce a plurality of tagged nucleic acid strands; (g) contacting the plurality of tagged nucleic acid strands with a probe set to create an enriched library; and (h) amplifying the enriched library.
Embodiment 3. The method of embodiment 1 or 2, wherein the sample comprises a sample from a mammal.
Embodiment 4. The method of embodiment 3, wherein the sample comprises a sample from a human, monkey, bat, dog, cat, horse, goat, sheep, cow, pig, rat and/or mouse.
Embodiment 5. The method of any one of embodiments 1-4, wherein the sample comprises a blood sample, a serum sample, and/or a whole blood sample.
Embodiment 6. The method of any one of embodiments 1-4, wherein the sample comprises a tissue sample.
Embodiment 7. The method of any one of embodiments 1-4, wherein the sample comprises a fecal sample, a urine sample, a mucus sample, a saliva sample, a lymph sample, a vaginal fluid sample, a semen sample, an amniotic sample, and/or a sweat sample.
Embodiment 8. The method of embodiment 1 or 2, comprises a freshwater sample, a wastewater sample, a saline water sample, or a combination thereof.
Embodiment 9. The method of embodiment 1 or 8, wherein the sample comprises a wastewater sample.
Embodiment 10. The method of any one of embodiments 1-9, wherein the probe set is biotinylated.
Embodiment 11. The method of any one of embodiments 1-10, wherein the one or more target coronavirus nucleic acids are coronavirus RNA molecules.
Embodiment 12. The method of any one of embodiments 1-11, wherein the one or more target coronavirus nucleic acids are genomic coronavirus RNA molecules.
Embodiment 13. The method of any one of embodiments 1-12, wherein the probe set further comprises at least two DNA probes that each hybridize to at least one target coronavirus molecule of the Alphacoronavirus, Betacoronavirus, Deltacoronavirus, Gammacoronavirus, and/or Bafinivirus genus.
Embodiment 14. The method of any one of embodiments 1-13, wherein the probe set further comprises at least two DNA probes that each hybridize to at least one target coronavirus molecule selected from Table 2.
Embodiment 15. The method of any one of embodiments 1-14, wherein at wherein the DNA probes further comprise any one of SEQ ID NOs 22917-23376.
Embodiment 16. The method of any one of embodiments 1-15, wherein at wherein the DNA probes further comprise two or more, or five or more, or 10 or more, or 25 or more sequences, or all of the sequences selected from SEQ ID NOs: 22917-23376.
Embodiment 17. The method of any one of embodiments 1-16, wherein the method further comprises depleting unwanted nucleic acid molecules from a nucleic acid sample.
Embodiment 18. The method of any one of embodiments 1-17, wherein the depleting unwanted nucleic acid molecules comprises depleting unwanted cDNA library fragments from a library of cDNA fragments prepared from RNA, wherein the unwanted library fragments comprise those prepared from unwanted RNA sequences, further comprising: (a) preparing a solid support comprising at least one immobilized oligonucleotide, wherein each immobilized oligonucleotide comprises a nucleic acid sequence corresponding to an unwanted RNA sequence or its complement; (b) adding the library of fragments to the solid support and hybridizing the library fragments to at least one immobilized oligonucleotide to allow binding of unwanted library fragments to at least one immobilized oligonucleotide; and (c) collecting library fragments not bound to at least one immobilized oligonucleotide.
Embodiment 19. The method of claim any one of embodiments 1-18 wherein the at least one immobilized oligonucleotide comprises a sequence comprising any one or more of SEQ ID NOs: 23377-24507 or its complement.
Embodiment 20. The method of any one of embodiments 1-19, wherein the depleting unwanted nucleic acid molecules comprises depleting off-target RNA nucleic acid molecules from a nucleic acid sample comprises: (a) contacting a nucleic acid sample comprising at least one RNA or DNA target sequence and at least one off-target RNA molecule from a first species with a probe set comprising at least two DNA probes complementary to discontiguous sequences along the full length of the at least one off-target RNA molecule from a second species, thereby hybridizing the DNA probes to the off-target RNA molecules to form DNA: RNA hybrids, wherein each DNA: RNA hybrid is at least 5 bases apart, or at least 10 bases apart, along a given off-target RNA molecule sequence from any other DNA: RNA hybrid, wherein the off-target DNA comprises at least one small noncoding RNA chosen from RN7SK, RN7SL1, RN7SL2, RN7SL5P, RPPH1, SNORD3A; (b) contacting the DNA: RNA hybrids with a ribonuclease that degrades the RNA from the DNA: RNA hybrids, thereby degrading the off-target RNA molecules in the nucleic acid sample to form a degraded mixture; (c) separating the degraded RNA from the degraded mixture; (d) sequencing the remaining RNA from the sample; (e) evaluating the remaining RNA sequences for the presence of off-target RNA molecules from the first species, thereby determining gap sequence regions; and (f) supplementing the probe set with additional DNA probes complementary to discontiguous sequences in one or more of the gap sequence regions.
Embodiment 21. The method of any one of embodiments 1-20, wherein the probe set comprises any one or more of SEQ ID NOs: 22917-23376.
Embodiment 22. The method of any one of embodiments 1-21, wherein the method further comprises depleting unwanted cDNA library fragments from a library of cDNA fragments prepared from RNA, wherein the unwanted library fragments comprise those prepared from unwanted RNA sequences.
Embodiment 23. A composition comprising a probe set comprising at least two DNA probes complementary to at least one target coronavirus RNA molecule in a nucleic acid sample wherein the target coronavirus RNA comprises at least one coronavirus molecule selected from Table 2.
Embodiment 24. A composition comprising a probe set comprising at least one DNA probe comprising at least one sequence of SEQ ID NOs: 1-22909.
Embodiment 25. The composition of embodiment 24, comprising at least 5, at least at least 10, at least 50, at least 100, at least 250, at least 500, at least 750, at least 1000, at least 1500, or at least 2000 sequences of SEQ ID NOs: 1-22909.
Embodiment 26. A kit comprising a probe set comprising: (a) at least one DNA probe comprising at least one sequence comprising at least one of SEQ ID NOs: 1-22909; and (b) a buffer.
Embodiment 27. The kit of any one of embodiment 26, wherein the buffer is a wash buffer and/or an elution buffer.
Embodiment 28. The kit of any one of embodiment 26 or 27, further comprising an RNA depletion buffer, a probe depletion buffer, and/or a probe removal buffer.
Embodiment 29. The kit of any one of embodiments 26-28, further comprising: (a) a ribonuclease; (b) a DNase; and (c) RNA purification beads.
Embodiment 30. The kit of any one of embodiments 26-29, wherein the ribonuclease is RNase H.
Embodiment 31. The kit of any one of embodiments 26-30, comprising a buffer and nucleic acid purification medium.
Embodiment 32. The kit of any one of embodiments 26-31, wherein the buffer is an RNA depletion buffer, a probe depletion buffer, and/or a probe removal buffer.
Embodiment 33. The kit of any one of embodiments 26-32. further comprising a nucleic acid destabilizing chemical.
Embodiment 34. The kit of embodiment 33, wherein the nucleic acid destabilizing chemical comprises betaine, DMSO, formamide, glycerol, or a derivative thereof, or a mixture thereof.
Embodiment 35. The kit of embodiment 33 or 34, wherein the nucleic acid destabilizing chemical comprises formamide.
Embodiment 36. The kit of any one of embodiments 26-35, wherein the at least one DNA probe comprises 2 or more, 5 or more, 10 or more, 25 or more, 50 or more, 100 or more, 200 or more, 300 or more, 400 or more, 500 or more, 600 or more, 700 or more, 800 or more, 900 or more, 1000 or more, 1100 or more, or 22909 probes comprising sequences selected from SEQ ID NOs: 1-22909.
Embodiment 37. The kit of any one of embodiments 26-36, wherein the at least one DNA probe comprises 500 or more, 600 or more, 700 or more, 800 or more, 900 or more, 1000 or more, 1100 or more, or 22909 probes comprising sequences selected from SEQ ID NOs: 1-22909.
Additional objects and advantages will be set forth in part in the description which follows, and in part will be understood from the description, or may be learned by practice. The objects and advantages will be realized and attained by means of the elements and combinations particularly pointed out in the appended claims.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the claims.
BRIEF DESCRIPTION OF SELECT SEQUENCES
|
SEQ
|
ID
|
Description
NO:
Sequence (3′ to 5′)
|
|
RN7SK
22910
GATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG
|
GGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCT
|
CACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGG
|
ACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGA
|
GTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAGGTCCAT
|
TTGTAGGAGAACGTAGGGTAGTCAAGCTTCCAAGACTCCAGACACATCC
|
AAATGAGGCGCTGCATGTGGCAGTCTGCCTTTCT
|
|
RN7SL1
22911
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGA
|
GGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATG
|
CCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG
|
GAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC
|
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG
|
CCTGTGAATAGCCACTGCACTCCAGCCTGGGCAACATAGCGAGACCCCG
|
TCTCT
|
|
RN7SL2
22912
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGA
|
GGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATG
|
CCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG
|
GAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC
|
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG
|
CCTGTGAATAGCCACTGCACTCCAGCCTGAGCAACATAGCGAGACCCCG
|
TCTCTT
|
|
RN7SL5P
22913
GCCGGGCGCGGTGGCGCGTGCCTGTGGTCCCAGCTACTCGGGAGGCTGA
|
GGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATG
|
CCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG
|
GAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC
|
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGAAGTCTGTAA
|
TGCTACTGGTGTCCCCTAATTTTCTTATAGCCACAGTTCCTTTCGCCTG
|
AGCTCATTACAGAGACAAATATCCATT
|
|
RPPH1
22914
GGCGGAGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCA
|
CTCCACTCCCATGTCCCTTGGGAAGGTCTGAGACTAGGGCCAGAGGCGG
|
CCCTAACAGGGCTCTCCCTGAGCTTCGGGGAGGTGAGTTCCCAGAGAAC
|
GGGGCTCCGCGCGAGGTCAGACTGGGCAGGAGATGCCGTGGACCCCGCC
|
CTTCGGGGAGGGGCCCGGCGGATGCCTCCTTTGCCGGAGCTTGGAACAG
|
ACTCACGGCCAGCGAAGTGAGTTCAATGGCTGAGGTGAGGTACCCCGCA
|
GGGGACCTCATAACCCAATTCAGACTACTCTCCTCCGCC
|
|
SNORD3A with
22915
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTT
|
the ALU region in
TCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTT
|
bold and italics, in
TTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGGCTGCAACTGC
|
some
CGTCAGCCATTGATGATCGTTCTTCTCTCCGTATTGGGGAGTGAGAGGG
|
embodiments the
AGAGAACGCGGTCTGAGTGGTTTTTCCTTCTTGATGGCTCAATGACAGA
|
ALU region was
GACTAGCTCGTAAACTCCGGGGCGTTTCTGGGCTGTTCGCTCCTGCTTG
|
not used to
GCATGTCGCGAGAAAGGTTTTCGCCTCCTGTTTCAGCGGTGACGGCTCT
|
generate probes
TGGGTTTTCTCGGGGTGGCTTTTTAATTTTAGTCTTGGCGCGAGGCGGG
|
because it is a
GGATGCTGTGTGGCACCTCCTATTGTCTCTTTTTGCGTTTTCTCCCATT
|
repetitive region in
CTCGCTCCCTCTTTTGTCGCCGTTTCCCGCCCGCCACTCCCACCCCCAG
|
other areas of the
ACGGGGTCTCCGGGTCTCTTGTTCTGTCTGCCGGCCCCGGCTGGATTGC
|
genome.
AGTGGCGCGATCTCGGCTCCTAGCAACATCTGCCTCCCGGGCTCAAGCG
|
AGTCTCCCGCCTAAGCCCTCCCGAGTAGCCGGGGCTTAAAGGCGCACAC
|
GCCACTCCAGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAGAAACG
|
GGGTGTCAGCATG
|
|
Reverse
22916
AGAAAGGCAGACTGCCACATGCAGCGCCTCATTTGGATGTGTCTGGAGT
|
complement of
C
TTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGC
|
RN7SK with
TTGTTTGGAGGTTCTAG
CAGGGGAGCGCAGCTACTCGTATACCCTTGAC
|
probe sequences in
CGAAGACCGGTCCTCCTCTATCGGGGATGGTCG
TCCTCTTCGACCGAGC
|
bold and italics
GCGCAGCTTCGGGAGGGACGCACATGGAGCGGTGAGGGAGGAAGGGGAC
|
(and with gaps
ACCCGCCTAGCCAGCCAGATCAGCCGAATCAACCCTGGCGATCAATGGG
|
between the probes)
GTGACAGATGTCGCAG
CCAGATCGCCCTCACATC
|
|
Probe for
22917
AGAAAGGCAGACTGCCACATGCAGCGCCTCATTTGGATGTGTCTGGAGT
|
RN7SK
C
|
|
Probe for
22918
CCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTA
|
RN7SK
G
|
|
Probe for
22919
ACTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTC
|
RN7SK
G
|
|
Probe for
22920
CGCGCAGCTTCGGGAGGGACGCACATGGAGCGGTGAGGGAGGAAGGGGA
|
RN7SK
C
|
|
Probe for
22921
CAGATCAGCCGAATCAACCCTGGCGATCAATGGGGTGACAGATGTCGCA
|
RN7SK
G
|
|
Probe
22922
AGAGACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTC
|
for RN7SL1
A
|
|
Probe for
22923
TACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG
|
RN7SL1
G
|
|
Probe for
22924
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA
|
RN7SL1
C
|
|
Probe for
22925
GATCGGCATAGCGCACTACAGCCCAGAACTCCTGGACTCAAGCGATCCT
|
RN7SL1
C
|
|
Probe for RN7SL2
22926
AAGAGACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATT
|
C
|
|
Probe for RN7SL2
22927
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCC
|
G
|
|
Probe for RN7SL2
22928
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA
|
A
|
|
Probe for RN7SL2
22929
CGATCGGCATAGCGCACTACAGCCCAGAACTCCTGGGCTCAAGCGATCC
|
T
|
|
Probe
22930
AATGGATATTTGTCTCTGTAATGAGCTCAGGCGAAAGGAACTGTGGCTA
|
for RN7SL5P
T
|
|
Probe
22931
CACCAGTAGCATTACAGACTTCTACTGATCAGCACGGGAGTTTTGACCT
|
for RN7SL5P
G
|
|
Probe
22932
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGG
|
for RN7SL5P
A
|
|
Probe
22933
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGA
|
for RN7SL5P
A
|
|
Probe
22934
GATCCTCCAGCCTCAGCCTCCCGAGTAGCTGGGACCACAGGCACGCGCC
|
for RN7SL5P
A
|
|
Probe for RPPH1
22935
GGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC
|
C
|
|
Probe for RPPH1
22936
AACTCACTTCGCTGGCCGTGAGTCTGTTCCAAGCTCCGGCAAAGGAGGC
|
A
|
|
Probe for RPPH1
22937
CCCGAAGGGCGGGGTCCACGGCATCTCCTGCCCAGTCTGACCTCGCGCG
|
G
|
|
Probe for RPPH1
22938
GAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAGGGCCGCCTCTG
|
G
|
|
Probe for RPPH1
22939
TTCCCAAGGGACATGGGAGTGGAGTGACAGGACGCACTCAGCTCGTGGC
|
C
|
|
Probe
22940
CCCGGAGACCCCGTCTGGGGGTGGGAGTGGCGGGCGGGAAACGGCGACA
|
for SNORD3A
A
|
|
Probe
22941
TGGGAGAAAACGCAAAAAGAGACAATAGGAGGTGCCACACAGCATCCCC
|
for SNORD3A
C
|
|
Probe
22942
TAAAATTAAAAAGCCACCCCGAGAAAACCCAAGAGCCGTCACCGCTGAA
|
for SNORD3A
A
|
|
Probe
22943
TTTCTCGCGACATGCCAAGCAGGAGCGAACAGCCCAGAAACGCCCCGGA
|
for SNORD3A
G
|
|
Probe
22944
CTGTCATTGAGCCATCAAGAAGGAAAAACCACTCAGACCGCGTTCTCTC
|
for SNORD3A
C
|
|
Probe for
22945
ACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCA
|
SNORD3A
A
|
|
Probe for
22946
TTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGC
|
SNORD3A
T
|
|
Probe for
22947
AAACTTCTCTAGTAACACACTATAGAAATGATCCCTGAAAGTATAGTCT
|
SNORD3A
T
|
(additional probe
|
added at start of
|
SNORD3A
|
transcript)
|
|
Probe for RN7SL1
22948
CTCAGCCTCCCGAGTAGCTGGGACTACAGGCACGCGCCACCGCGCCCGG
|
and RN7SL2
C
|
(additional probe
|
added at start of
|
RN7SL1 and
|
RN7SL2
|
transcript)
|
|
Additional Probes
|
12S_P1
22949
GTTCGTCCAAGTGCACTTTCCAGTACACTTACCATGTTACGACTTGTCT
|
C
|
|
12S_P2
22951
TAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAGGAGGGTGACG
|
G
|
|
12S_P3
22951
TTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT
|
C
|
|
12S_P4
22952
AGTTTCATAAGGGCTATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCAT
|
T
|
|
12S_P5
22953
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG
|
T
|
|
12S_P6
22954
TTGCTGAAGATGGCGGTATATAGGCTGAGCAAGAGGTGGTGAGGTTGAT
|
C
|
|
12S_P7
22955
CAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAG
|
T
|
|
12S_P8
22956
GTAGTGTTCTGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGG
|
C
|
|
12S_P9
22957
ATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAA
|
G
|
|
12S_P10
22958
ATTTTGTGTCAACTGGAGTTTTTTACAACTCAGGTGAGTTTTAGCTTTA
|
T
|
|
12S_P11
22959
CTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTGA
|
C
|
|
12S_P12
22960
GAAATTGACCAACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTA
|
T
|
|
12S_P13
22961
ACTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCA
|
T
|
|
12S_P14
22962
GCTTGTCCCTTTTGATCGTGGTGATTTAGAGGGTGAACTCACTGGAACG
|
G
|
|
12S_P15
22963
TAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCTATTTGTTT
|
A
|
|
16S_P1
22964
AAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATA
|
T
|
|
16S_P2
22965
GCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG
|
A
|
|
16S_P3
22966
AAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTA
|
A
|
|
16S_P4
22967
ACCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGG
|
T
|
|
16S_P5
22968
TGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG
|
T
|
|
16S_P6
22969
ATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGC
|
A
|
|
16S_P7
22970
TTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT
|
G
|
|
16S_P8
22971
TGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT
|
C
|
|
16S_P9
22972
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAA
|
G
|
|
16S_P10
22973
CGTGGAGCCATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCT
|
A
|
|
16S_P11
22974
GGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAAT
|
A
|
|
16S_P12
22975
GTGATGTTTTTGGTAAACAGGCGGGGTAAGGTTTGCCGAGTTCCTTTTA
|
C
|
|
16S_P13
22976
CTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG
|
T
|
|
16S_P14
22977
ATTGGGCTGTTAATTGTCAGTTCAGTGTTTTGATCTGACGCAGGCTTAT
|
G
|
|
16S_P15
22978
TCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGT
|
C
|
|
16S_P16
22979
AGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA
|
A
|
|
16S_P17
22980
TGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTAC
|
A
|
|
16S_P18
22981
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGA
|
T
|
|
16S_P19
22982
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCA
|
C
|
|
16S_P20
22983
TGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGT
|
G
|
|
16S_P21
22984
TCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTG
|
C
|
|
16S_P22
22985
TAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCT
|
T
|
|
16S_P23
22986
TCTTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATC
|
G
|
|
16S_P24
22987
GGTAAATGGTTTGGCTAAGGTTGTCTGGTAGTAAGGTGGAGTGGGTTTG
|
G
|
|
18S_P1
22988
TAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTA
|
C
|
|
18S_P2
22989
AAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCG
|
G
|
|
18S_P3
22990
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAA
|
A
|
|
18S_P4
22991
CAACGCAAGCTTATGACCCGCACTTACTCGGGAATTCCCTCGTTCATGG
|
G
|
|
18S_P5
22992
CCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGC
|
G
|
|
18S_P6
22993
CTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCAT
|
C
|
|
18S_P7
22994
CTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG
|
A
|
|
18S_P8
22995
GGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA
|
A
|
|
18S_P9
22996
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG
|
C
|
|
18S_P10
22997
CCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAATTAA
|
G
|
|
18S_P11
22998
CTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCAT
|
A
|
|
18S_P12
22999
AAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC
|
C
|
|
18S_P13
23000
GGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACC
|
T
|
|
18S_P14
23001
GATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGC
|
G
|
|
18S_P15
23002
CACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCAT
|
G
|
|
18S_P16
23003
ACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTA
|
T
|
|
18S_P17
23004
CTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCG
|
G
|
|
18S_P18
23005
GCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCG
|
C
|
|
18S_P19
23006
CCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTT
|
A
|
|
18S_P20
23007
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCC
|
T
|
|
18S_P21
23008
AGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTAT
|
T
|
|
18S_P22
23009
CCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT
|
G
|
|
18S_P23
23010
GCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA
|
T
|
|
18S_P24
23011
TACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCA
|
C
|
|
18S_P25
23012
GGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGG
|
C
|
|
18S_P26
23013
GCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGA
|
A
|
|
18S_P27
23014
TCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG
|
A
|
|
18S_P28
23015
AACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTACCGGCCGT
|
G
|
|
18S_P29
23016
ATGGCTTAATCTTTGAGACAAGCATATGCTACTGGCAGGATCAACCAGG
|
T
|
|
28S_P1
23017
GACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGA
|
G
|
|
28S_P2
23018
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGG
|
T
|
|
28S_P3
23019
GGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTT
|
C
|
|
28S_P4
23020
CTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCC
|
G
|
|
28S_P5
23021
AGGGGGGGGCGGCCCGCCGGCGGGGACAGGCGGGGGACCGGCTATCCGA
|
G
|
|
28S_P6
23022
GCGGCGCTGCCGTATCGTTCGCCTGGGCGGGATTCTGACTTAGAGGCGT
|
T
|
|
28S_P7
23023
AGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAA
|
T
|
|
28S_P8
23024
TCCTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAG
|
G
|
|
28S_P9
23025
CTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAA
|
T
|
|
28S_P10
23026
TTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCG
|
A
|
|
28S_P11
23027
TTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCC
|
T
|
|
28S_P12
23028
GGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGA
|
A
|
|
28S_P13
23029
AGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCG
|
C
|
|
28S_P14
23030
TTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACT
|
G
|
|
28S_P15
23031
GCGCCCGGCCGGGCGGGCGCTTGGCGCCAGAAGCGAGAGCCCCTCGGGC
|
T
|
|
28S_P16
23032
CCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCC
|
G
|
|
28S_P17
23033
CGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCA
|
C
|
|
28S_P18
23034
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCT
|
T
|
|
28S_P19
23035
CCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACA
|
G
|
|
28S_P20
23036
TCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTA
|
A
|
|
28S_P21
23037
TCCCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACATTCAG
|
A
|
|
28S_P22
23038
CACATCGCGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAAT
|
T
|
|
28S_P23
23039
CCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGA
|
G
|
|
28S_P24
23040
CGGCCCCGGGGGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCG
|
C
|
|
28S_P25
23041
CCGCCGCGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGGGCCGGG
|
G
|
|
28S_P26
23042
ACGAACCGCCCCGCCCCGCCGCCCGCCGACCGCCGCCGCCCGACCGCTC
|
C
|
|
28S_P27
23043
CGCGCGCGACCGAGACGTGGGGTGGGGGTGGGGGGCGCGCCGCGCCGCC
|
G
|
|
28S_P28
23044
GCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGAAGGGCCCGG
|
C
|
|
28S_P29
23045
GCGCCGCCGCCGGCCCCCCGGGTCCCCGGGGCCCCCCTCGCGGGGACCT
|
G
|
|
28S_P30
23046
CCGGCGGCCGCCGCGCGGCCCCTGCCGCCCCGACCCTTCTCCCCCCGCC
|
G
|
|
28S_P31
23047
CTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAGAGAGAG
|
A
|
|
28S_P32
23048
AGGGAGCGAGCGGCGCGCGCGGGTGGGGCGGGGGAGGGCCGCGAGGGGG
|
G
|
|
28S_P33
23049
GGGGGCGCGCGCCTCGTCCAGCCGCGGCGCGCGCCCAGCCCCGCTTCGC
|
G
|
|
28S_P34
23050
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC
|
C
|
|
28S_P35
23051
CATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGA
|
T
|
|
28S_P36
23052
CGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCT
|
C
|
|
28S_P37
23053
AACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCAT
|
T
|
|
28S_P38
23054
CTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCG
|
G
|
|
28S_P39
23055
CGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGA
|
T
|
|
28S_P40
23056
TTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACG
|
G
|
|
28S_P41
23057
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTC
|
C
|
|
28S_P42
23058
GTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCG
|
G
|
|
28S_P43
23059
CGCCCTAGGCTTCAAGGCTCACCGCAGCGGCCCTCCTACTCGTCGCGGC
|
G
|
|
28S_P44
23060
TCCGGGGGCGGGGAGCGGGGCGTGGGCGGGAGGAGGGGAGGAGGCGTGG
|
G
|
|
28S_P45
23061
AGGACCCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACA
|
C
|
|
28S_P46
23062
GCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCC
|
G
|
|
28S_P47
23063
CTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGT
|
T
|
|
28S_P48
23064
GATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACC
|
T
|
|
28S_P49
23065
GAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC
|
G
|
|
28S_P50
23066
AAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGC
|
G
|
|
28S_P51
23067
CCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC
|
T
|
|
28S_P52
23068
CGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGC
|
T
|
|
28S_P53
23069
TCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATA
|
C
|
|
28S_P54
23070
GATTTGCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGC
|
T
|
|
28S_P55
23071
ATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTC
|
C
|
|
28S_P56
23072
AGACGGGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCGGGATCC
|
C
|
|
28S_P57
23073
CGCGCCGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCC
|
C
|
|
28S_P58
23074
TTAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGGGTGGGTAGCCGACG
|
T
|
|
28S_P59
23075
GCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGA
|
G
|
|
28S_P60
23076
CCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCCCGCC
|
C
|
|
28S_P61
23077
GCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGGAGGGGTGGGAGA
|
G
|
|
28S_P62
23078
AGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGG
|
G
|
|
28S_P63
23079
GGGGATTCCCCGCGGGGGTGGGCGCCGGGAGGGGGGAGAGCGCGGCGAC
|
G
|
|
28S_P64
23080
GCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGG
|
G
|
|
28S_P65
23081
CCGCCCCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGCCCCGACCCG
|
C
|
|
28S_P66
23082
AGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGA
|
C
|
|
28S_P67
23083
AGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGG
|
C
|
|
28S_P68
23084
AAATGCGCCCGGCGGCGGCCGGTCGCCGGTCGGGGGACGGTCCCCCGCC
|
G
|
|
28S_P69
23085
CCGCCCGCCCACCCCCGCACCCGCCGGAGCCCGCCCCCTCCGGGGAGGA
|
G
|
|
28S_P70
23086
GGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAG
|
A
|
|
28S_P71
23087
ACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGG
|
A
|
|
28S_P72
23088
TCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA
|
A
|
|
28S_P73
23089
CTTGTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTA
|
C
|
|
28S_P74
23090
GCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCGCGCGCCGGCCG
|
C
|
|
28S_P75
23091
GTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGG
|
C
|
|
28S_P76
23092
TTCCGTACGCCACATGTCCCGCGCCCCGCGGGGCGGGGATTCGGCGCTG
|
G
|
|
28S_P77
23093
CTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTTTCCTCCGCTGACT
|
A
|
|
28S_P78
23094
GCGGGTCGCCACGTCTGATCTGAGGTCGCGTCTCGGAGGGGGACGGGCC
|
G
|
|
5.8S_P1
23095
AAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAG
|
T
|
|
5.8S_P3
23096
GCAGCTAGCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCT
|
A
|
|
5S_P1
23097
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTA
|
A
|
|
5S_P3
23098
TTCCGAGATCAGACGAGATCGGGCGCGTTCAGGGTGGTATGGCCGTAGA
|
C
|
|
HBA1_P1
23099
GCCGCCCACTCAGACTTTATTCAAAGACCACGGGGGTACGGGTGCAGGA
|
A
|
|
HBA1_P2
23100
GGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAA
|
C
|
|
HBA1_P3
23101
GCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGC
|
A
|
|
HBA1_P4
23102
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA
|
A
|
|
HBA1_P5
23103
CCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC
|
A
|
|
HBA1_P6
23104
CCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAAC
|
C
|
|
HBA1_P7
23105
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGA
|
A
|
|
HBA1_P8
23106
CTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCCAGGCGGCC
|
T
|
|
HBA1_P9
23107
CGGCAGGAGACAGCACCATGGTGGGTTCTCTCTGAGTCTGTGGGGACCA
|
G
|
|
HBA2_P1
23108
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTA
|
C
|
|
HBA2_P2
23109
ACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCC
|
A
|
|
HBA2_P3
23110
CAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCA
|
G
|
|
HBA2_P4
23111
AAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGG
|
C
|
|
HBA2_P5
23112
CATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC
|
T
|
|
HBA2_P6
23113
CCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGT
|
G
|
|
HBA2_P7
23114
AACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGA
|
C
|
|
HBA2_P8
23115
CTTGACGTTGGTCTTGTCGGCAGGAGACAGCACCATGGTGGGTTCTCTC
|
T
|
|
HBB_P1
23116
GCAATGAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGC
|
C
|
|
HBB_P2
23117
CAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTG
|
G
|
|
HBB_P3
23118
GCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTT
|
T
|
|
HBB_P4
23119
CACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC
|
A
|
|
HBB_P5
23120
GCCTGAAGTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCAGCTCACT
|
C
|
|
HBB_P6
23121
CCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCA
|
C
|
|
HBB_P7
23122
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCA
|
A
|
|
HBB_P8
23123
TCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCCTCACCACC
|
A
|
|
HBB_P9
23124
ACCTTGCCCCACAGGGCAGTAACGGCAGACTTCTCCTCAGGAGTCAGAT
|
G
|
|
HBG1_P1
23125
GTGATCTCTCAGCAGAATAGATTTATTATTTGTATTGCTTGCAGAATAA
|
A
|
|
HBG1_P2
23126
CTCTGAATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACT
|
G
|
|
HBG1_P3
23127
ATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAAT
|
G
|
|
HBG1_P4
23128
CACCAGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGC
|
T
|
|
HBG1_P5
23129
CACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTGTGGC
|
A
|
|
HBG1_P6
23130
AGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAG
|
A
|
|
HBG1_P7
23131
GCCAAAGCTGTCAAAGAACCTCTGGGTCCATGGGTAGACAACCAGGAGC
|
C
|
|
HBG1_P8
23132
CTCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGC
|
C
|
|
HBG1_P9
23133
AAATGACCCATGGCGTCTGGACTAGGAGCTTATTGATAACCTCAGACGT
|
T
|
|
HBG2_P1
23134
GTGATCTCTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAA
|
G
|
|
HBG2_P2
23135
TCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTG
|
G
|
|
HBG2_P3
23136
TCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAATG
|
G
|
|
HBG2_P4
23137
ACCAGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCT
|
T
|
|
HBG2_P5
23138
ACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCA
|
T
|
|
HBG2_P6
23139
GCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGCAGA
|
G
|
|
HBG2_P7
23140
CCAAAGCTGTCAAAGAACCTCTGGGTCCATGGGTAGACAACCAGGAGCC
|
T
|
|
HBG2_P8
23141
TCCAGCATCTTCCACATTCACCTTGCCCCACAGGCTTGTGATAGTAGCC
|
T
|
|
HBG2_P9
23142
AATGACCCATGGCGTCTGGACTAGGAGCTTATTGATAACCTCAGACGTT
|
C
|
|
5S_GNbac_P1
23143
ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATC
|
G
|
|
5S_GNbac_P2
23144
ACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGC
|
C
|
|
16S_GNbac_P1
23145
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAG
|
T
|
|
16S_GNbac_P2
23146
AAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTG
|
T
|
|
16S_GNbac_P3
23147
ACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTT
|
C
|
|
16S_GNbac_P4
23148
AGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCG
|
C
|
|
16S_GNbac_P5
23149
TGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGA
|
T
|
|
16S_GNbac_P6
23150
CCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCG
|
G
|
|
16S_GNbac_P7
23151
GGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACAC
|
G
|
|
16S_GNbac_P8
23152
TGCAGCACCTGTCTCACGGTTCCCGAAGGCACATTCTCATCTCTGAAAA
|
C
|
|
16S_GNbac_P9
23153
GACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCAC
|
C
|
|
16S_GNbac_P10
23154
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTC
|
G
|
|
16S_GNbac_P11
23155
TCCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCGTTTAC
|
G
|
|
16S_GNbac_P12
23156
GTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTGAGCGTCAGTCTT
|
C
|
|
16S_GNbac_P13
23157
TTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT
|
G
|
|
16S_GNbac_P14
23158
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCC
|
C
|
|
16S_GNbac_P15
23159
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAA
|
C
|
|
16S_GNbac_P16
23160
ATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTA
|
A
|
|
16S_GNbac_P17
23161
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA
|
A
|
|
16S_GNbac_P18
23162
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAGTATTCCCCACT
|
G
|
|
16S_GNbac_P19
23163
GTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCA
|
G
|
|
16S_GNbac_P20
23164
TAGGTGAGCCGTTACCCCACCTACTAGCTAATCCCATCTGGGCACATCC
|
G
|
|
16S_GNbac_P21
23165
AAGGTCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTT
|
T
|
|
16S_GNbac_P22
23166
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA
|
G
|
|
23S_GNbac_P1
23167
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCT
|
G
|
|
23S_GNbac_P2
23168
CCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTC
|
A
|
|
23S_GNbac_P3
23169
GGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT
|
T
|
|
23S_GNbac_P4
23170
CCATTGGCATGACAACCCGAACACCAGTGATGCGTCCACTCCGGTCCTC
|
T
|
|
23S_GNbac_P5
23171
CCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCA
|
C
|
|
23S_GNbac_P6
23172
GCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACT
|
T
|
|
23S_GNbac_P7
23173
ATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGG
|
G
|
|
23S_GNbac_P8
23174
ATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAA
|
C
|
|
23S_GNbac_P9
23175
ACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCT
|
T
|
|
23S_GNbac_P10
23176
CCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTAC
|
T
|
|
23S_GNbac_P11
23177
GCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG
|
T
|
|
23S_GNbac_P12
23178
AAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTC
|
C
|
|
23S_GNbac_P13
23179
CCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA
|
A
|
|
23S_GNbac_P14
23180
TTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCA
|
C
|
|
23S_GNbac_P15
23181
GACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTACCCGA
|
C
|
|
23S_GNbac_P16
23182
CTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG
|
A
|
|
23S_GNbac_P17
23183
ACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACG
|
T
|
|
23S_GNbac_P18
23184
CACAGTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGA
|
C
|
|
23S_GNbac_P19
23185
CCGCGAGGGACCTCACCTACATATCAGCGTGCCTTCTCCCGAAGTTACG
|
G
|
|
23S_GNbac_P20
23186
TTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCA
|
C
|
|
23S_GNbac_P21
23187
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGG
|
C
|
|
23S_GNbac_P22
23188
ACCGTAGTGCCTCGTCATCACGCCTCAGCCTTGATTTTCCGGATTTGCC
|
T
|
|
23S_GNbac_P23
23189
ACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCCGTC
|
C
|
|
23S_GNbac_P24
23190
ACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGC
|
C
|
|
23S_GNbac_P25
23191
ACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCCGGC
|
G
|
|
23S_GNbac_P26
23192
CGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCA
|
T
|
|
23S_GNbac_P27
23193
TTCGCAGGCTTACAGAACGCTCCCCTACCCAACAACGCATAAGCGTCGC
|
T
|
|
23S_GNbac_P28
23194
CATGGTTTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGA
|
G
|
|
23S_GNbac_P29
23195
TAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTCCC
|
A
|
|
23S_GNbac_P30
23196
AACCATGACTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTC
|
A
|
|
23S_GNbac_P31
23197
CCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAGTTTGCA
|
T
|
|
23S_GNbac_P32
23198
GGATGACCCCCTTGCCGAAACAGTGCTCTACCCCCGGAGATGAATTCAC
|
G
|
|
23S_GNbac_P33
23199
AGCTTTCGGGGAGAACCAGCTATCTCCCGGTTTGATTGGCCTTTCACCC
|
C
|
|
23S_GNbac_P34
23200
CGCTAATTTTTCAACATTAGTCGGTTCGGTCCTCCAGTTAGTGTTACCC
|
A
|
|
23S_GNbac_P35
23201
ATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCA
|
G
|
|
23S_GNbac_P36
23202
CCTTCGGCTCCCCTATTCGGTTAACCTTGCTACAGAATATAAGTCGCTG
|
A
|
|
23S_GNbac_P37
23203
GTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGG
|
T
|
|
23S_GNbac_P38
23204
ACTCCCCTCGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCA
|
C
|
|
23S_GNbac_P39
23205
AGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCAC
|
G
|
|
23S_GNbac_P40
23206
ATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTA
|
T
|
|
23S_GNbac_P41
23207
ACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGT
|
T
|
|
23S_GNbac_P42
23208
GGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGT
|
T
|
|
23S_GNbac_P43
23209
ATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT
|
T
|
|
23S_GNbac_P44
23210
GCCGGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAG
|
C
|
|
5S_GPbac_P1
23211
GCTTGGCGGCGTCCTACTCTCACAGGGGGAAACCCCCGACTACCATCGG
|
C
|
|
5S_GPbac_P2
23212
TTCCGTGTTCGGTATGGGAACGGGTGTGACCTCTTCGCTATCGCCACCA
|
A
|
|
16S_GPbac_P1
23213
TAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTGTTAC
|
G
|
|
16S_GPbac_P2
23214
TCTGTCCCACCTTCGGCGGCTGGCTCCTAAAAGGTTACCTCACCGACTT
|
C
|
|
16S_GPbac_P3
23215
TCGTGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGC
|
G
|
|
16S_GPbac_P4
23216
ATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCG
|
A
|
|
16S_GPbac_P5
23217
GTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATT
|
G
|
|
16S_GPbac_P6
23218
CCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCG
|
G
|
|
16S_GPbac_P7
23219
CACCTTAGAGTGCCCAACTGAATGCTGGCAACTAAGATCAAGGGTTGCG
|
C
|
|
16S_GPbac_P8
23220
ACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCA
|
C
|
|
16S_GPbac_P9
23221
GACGTCCTATCTCTAGGATTGTCAGAGGATGTCAAGACCTGGTAAGGTT
|
C
|
|
16S_GPbac_P10
23222
ATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTG
|
A
|
|
16S_GPbac_P11
23223
CCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGG
|
G
|
|
16S_GPbac_P12
23224
ACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG
|
T
|
|
16S_GPbac_P13
23225
TCGCTCCTCAGCGTCAGTTACAGACCAGAGAGTCGCCTTCGCCACTGGT
|
G
|
|
16S_GPbac_P14
23226
ACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTCA
|
A
|
|
16S_GPbac_P15
23227
ATGACCCTCCCCGGTTGAGCCGGGGGCTTTCACATCAGACTTAAGAAAC
|
C
|
|
16S_GPbac_P16
23228
ACGCCCAATAATTCCGGACAACGCTTGCCACCTACGTATTACCGCGGCT
|
G
|
|
16S_GPbac_P17
23229
CCGTGGCTTTCTGGTTAGGTACCGTCAAGGTACCGCCCTATTCGAACGG
|
T
|
|
16S_GPbac_P18
23230
ACAACAGAGCTTTACGATCCGAAAACCTTCATCACTCACGCGGCGTTGC
|
T
|
|
16S_GPbac_P19
23231
CCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGT
|
G
|
|
16S_GPbac_P20
23232
GGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCG
|
T
|
|
16S_GPbac_P21
23233
CTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTA
|
T
|
|
16S_GPbac_P22
23234
TTCAAACAACCATCCGGTATTAGCCCCGGTTTCCCGGAGTTATCCCAGT
|
C
|
|
16S_GPbac_P23
23235
CCACGTGTTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCCCA
|
T
|
|
16S_GPbac_P24
23236
GCATGTATTAGGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACT
|
C
|
|
23S_GPbac_P1
23237
TGGTTAAGTCCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCAC
|
A
|
|
23S_GPbac_P2
23238
TATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAA
|
A
|
|
23S_GPbac_P3
23239
GGCTTCATGCTTAGATGCTTTCAGCACTTATCCCGTCCGCACATAGCTA
|
C
|
|
23S_GPbac_P4
23240
GCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTAC
|
T
|
|
23S_GPbac_P5
23241
CAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGT
|
T
|
|
23S_GPbac_P6
23242
GTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACTGACTACAGCC
|
C
|
|
23S_GPbac_P7
23243
CGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGA
|
T
|
|
23S_GPbac_P8
23244
GGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCG
|
G
|
|
23S_GPbac_P9
23245
TTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCC
|
T
|
|
23S_GPbac_P10
23246
GATTTCCAACCATTCTGAGGGAACCTTTGGGCGCCTCCGTTACCTTTTA
|
G
|
|
23S_GPbac_P11
23247
GTCAAACTGCCCACCTGACACTGTCTCCCCGCCCGATAAGGGCGGCGGG
|
T
|
|
23S_GPbac_P12
23248
GCCAGGGTAGTATCCCACCGATGCCTCCACCGAAGCTGGCGCTCCGGTT
|
T
|
|
23S_GPbac_P13
23249
ATCCTGTACAAGCTGTACCAACATTCAATATCAGGCTGCAGTAAAGCTC
|
C
|
|
23S_GPbac_P14
23250
CCTGTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGT
|
C
|
|
23S_GPbac_P15
23251
GCCCAGATCGTTGCGCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGA
|
A
|
|
23S_GPbac_P16
23252
ACCGTTATAGTTACGGCCGCCGTTTACTGGGGCTTCAATTCGCACCTTC
|
G
|
|
23S_GPbac_P17
23253
CCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCCT
|
T
|
|
23S_GPbac_P18
23254
CCTGTGTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCT
|
C
|
|
23S_GPbac_P19
23255
CAGAGCACCCCTTCTCCCGAAGTTACGGGGTCATTTTGCCGAGTTCCTT
|
A
|
|
23S_GPbac_P20
23256
ATCACCTTAGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGG
|
G
|
|
23S_GPbac_P21
23257
TAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTATATTT
|
C
|
|
23S_GPbac_P22
23258
TCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGG
|
A
|
|
23S_GPbac_P23
23259
CCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGG
|
A
|
|
23S_GPbac_P24
23260
TCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTAGGTCCCG
|
A
|
|
23S_GPbac_P25
23261
CGAGCCTTCCTCAGGAAACCTTAGGCATTCGGTGGAGGGGATTCTCACC
|
C
|
|
23S_GPbac_P26
23262
TACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCT
|
T
|
|
23S_GPbac_P27
23263
GCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTT
|
T
|
|
23S_GPbac_P28
23264
TCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGG
|
T
|
|
23S_GPbac_P29
23265
AACATCCTGGTTGTCTAAGCAACTCCACATCCTTTTCCACTTAACGTAT
|
A
|
|
23S_GPbac_P30
23266
TGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG
|
T
|
|
23S_GPbac_P31
23267
AAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGC
|
C
|
|
23S_GPbac_P32
23268
GCTCTACCTCCAAGACTCTTACCTTGAGGCTAGCCCTAAAGCTATTTCG
|
G
|
|
23S_GPbac_P33
23269
TCCAGGTTCGATTGGCATTTCACCCCTACCCACACCTCATCCCCGCACT
|
T
|
|
23S_GPbac_P34
23270
TTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG
|
A
|
|
23S_GPbac_P35
23271
TCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGG
|
C
|
|
23S_GPbac_P36
23272
TAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACG
|
C
|
|
23S_GPbac_P37
23273
GGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCC
|
T
|
|
23S_GPbac_P38
23274
ACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTA
|
G
|
|
23S_GPbac_P39
23275
CTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCA
|
C
|
|
23S_GPbac_P40
23276
GTTTTGACTACAGGGCTGTTACCTCCTATGGCGGGCCTTTCCAGACCTC
|
T
|
|
23S_GPbac_P41
23277
CTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCCTCT
|
T
|
|
23S_GPbac_P42
23278
CGTTTCGCTCGCCGCTACTCAGGGAATCGCATTTGCTTTCTCTTCCTCC
|
G
|
|
23S_GPbac_P43
23279
CAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATA
|
C
|
|
23S_GPbac_P44
23280
GGTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTTGCTTACAGCT
|
C
|
|
23S_GPbac_P45
23281
TGTTCGTCCCGTCCTTCATCGGCTCCTAGTGCCAAGGCATCCACCGTGC
|
G
|
|
16S:A1
23282
AAACTAGATTCGAATATAACAAAACATTACATCCTCATCCAATCCCTTT
|
T
|
|
16S:A2
23283
GCGGTGTGTGCAAGGAGCAGGGACGTATTCACCGCGCGATTGTGACACG
|
C
|
|
16S:A3
23284
GCCTTTCGGCGTCGGAACCCATTGTCTCAGCCATTGTAGCCCGCGTGTT
|
G
|
|
16S:A4
23285
GCATACGGACCTACCGTCGTCCACTCCTTCCTCCTATTTATCATAGGCG
|
G
|
|
16S:A5
23286
CGGCATCCAAAAAAGGATCCGCTGGTAACTAAGAGCGTGGGTCTCGCTC
|
G
|
|
16S:A6
23287
CAACCTGGCTATCATACAGCTGTCGCCTCTGGTGAGATGTCCGGCGTTG
|
A
|
|
16S:A7
23288
AGGCTCCACGCGTTGTGGTGCTCCCCCGCCAATTCCTTTAAGTTTCAGT
|
C
|
|
16S:A8
23289
CCAGGCGGCGGACTTAACAGCTTCCCTTCGGCACTGGGACAGCTCAAAG
|
C
|
|
16S:A9
23290
TCCGCATCGTTTACAGCTAGGACTACCCGGGTATCTAATCCGGTTCGCG
|
C
|
|
16S:A10
23291
TTCCCACAGTTAAGCTGCAGGATTTCACCAGAGACTTATTAAACCGGCT
|
A
|
|
16S:A12
23292
CTCTTATTCCAAAAGCTCTTTACACTAATGAAAAGCCATCCCGTTAAGA
|
A
|
|
16S:A13
23293
CCCCCGTCGCGATTTCTCACATTGCGGAGGTTTCGCGCCTGCTGCACCC
|
C
|
|
16S:A14
23294
TTGTCTCAGGTTCCATCTCCGGGCTCTTGCTCTCACAACCCGTACCGAT
|
C
|
|
16S:A16
23295
CATTACCTAACCAACTACCTAATCGGCCGCAGACCCATCCTTAGGCGAA
|
A
|
|
16S:A17
23296
AAACCATTACAGGAATAATTGCCTATCCAGTATTATCCCCAGTTTCCCA
|
G
|
|
16S:A18
23297
AAGGGTAGGTTATCCACGTGTTACTGAGCCGTACGCCACGAGCCTAAAC
|
T
|
|
23S:A1
23298
ACCTAGCGCGTAGCTGCCCGGCACTGCCTTATCAGACAACCGGTCGACC
|
A
|
|
23S:A2
23299
CGTTCCTCTCGTACTGGAGCCACCTTCCCCTCAGACTACTAACACATCC
|
A
|
|
23S:A3
23300
CCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCCTTTAATGGGCG
|
A
|
|
23S:A4
23301
GGTGCTGCTGCACACCCAGGATGGAAAGAACCGACATCGAAGTAGCAAG
|
C
|
|
23S:A5
23302
GGCTCTTGCCTGCGACCACCCAGTTATCCCCGAGGTAGTTTTTCTGTCA
|
T
|
|
23S:A6
23303
AGGAGGACTCTGAGGTTCGCTAGGCCCGGCTTTCGCCTCTGGATTTCTT
|
G
|
|
23S:A7
23304
CAAAGTAAGTTAGAAACACAGTCATAAGAAAGTGGTGTCTCAAGAACGA
|
A
|
|
23S:A8
23305
GACTTATAATCGAATTCTCCCACTTACACTGCATACCTATAACCAAGCT
|
T
|
|
23S:A9
23306
GTAAAACTCTACGGGGTCTTCGCTTCCCAATGGAAGACTCTGGCTTGTG
|
C
|
|
23S:A10
23307
TCACTAAGTTCTAGCTAGGGACAGTGGGGACCTCGTTCTACCATTCATG
|
C
|
|
23S:A11
23308
CGACAAGGCATTTCGCTACCTTAAGAGGGTTATAGTTACCCCCGCCGTT
|
T
|
|
23S:A12
23309
AACTGAACTCCAGCTTCACGTGCCAGCACTGGGCAGGTGTCGCCCTCTG
|
T
|
|
23S:A13
23310
CTAGCAGAGAGCTATGTTTTTATTAAACAGTCGGGCCCCCCTAGTCACT
|
G
|
|
23S:A14
23311
TTAAAACGCCTTAGCCTACTCAGCTAGGGGCACCTGTGACGGATCTCGG
|
T
|
|
23S:A15
23312
ACAAAACTAACTCCCTTTTCAAGGACTCCATGAATCAGTTAAACCAGTA
|
C
|
|
23S:A16
23313
ATAATGCCTACACCTGGTTCTCGCTATTACACCTCTCCCCAGGCTTAAA
|
C
|
|
23S:A17
23314
CAATCCTACAAAACATATCTCGAAGTGTCAGAAATTAGCCCTCAACGTC
|
A
|
|
23S:A18
23315
CTTTGCTGCTACTACTACCAGGATCCACATACCTGCAAGGTCCAAAGGA
|
A
|
|
23S:A19
23316
CAACCCACACAGGTCGCCACTCTACACAATCACCAAAAAAAAGGTGTTC
|
C
|
|
23S:A20
23317
GGATTAATTCCCGTCCATTTTAGGTGCCTCTGACCTCGATGGGTGATCT
|
G
|
|
23S:A21
23318
AGGGTGGCTGCTTCTAAGCCCACCTTCCCATTGTCTTGGGCCAAAGACT
|
C
|
|
23S:A22
23319
GTATTTAGGGGCCTTAACCATAGTCTGAGTTGTTTCTCTTTCGGGACAC
|
A
|
|
23S:A23
23320
CCTCACTCCAACCTTCTACGACGGTGACGAGTTCGGAGTTTTACAGTAC
|
G
|
|
23S:A24
23321
CCCTAAACGTCCAATTAGTGCTCTACCCCGCCACCAACCTCCAGTCAGG
|
C
|
|
23S:A25
23322
AATAGATCGACCGGCTTCGGGTTTCAATGCTGTGATTCCAGGCCCTATT
|
A
|
|
23S:A26
23323
ACAACGCTGCGGGCATATCGGTTTCCCTACGACTACAAGGATAAAAACC
|
T
|
|
23S:A27
23324
ACAAAGAACTCCCTGGCCCGTGTTTCAAGACGGACGATGCAACACTAGT
|
C
|
|
23S:A28
23325
ACAATGTTACCACTGATTCTTTCGGAAGAATTCATTCCTTACGCGCCAC
|
A
|
|
23S:A29
23326
CTGGTTTCAGGTACTTTTCACCCCCCTATAGGGGTACTTTTCAGCATTC
|
C
|
|
23S:A30
23327
CTCTATCGGTCTTGAGACGTATTTAGAATTGGAAGTTGATGCCTCCCAC
|
A
|
|
23S:A31
23328
ATCACCCTCTACGGTTCTAAAATTCCAAATAAAATTCGATTTATCCCAC
|
G
|
|
23S:A32
23329
TCTATACACCACATCTCCCTAATATTACTAAAAGGGATTCAGTTTGTTC
|
T
|
|
23S:A33
23330
GCCGTTACTAACGACATCGCATATTGCTTTCTTTTCCTCCGCCTACTAA
|
G
|
|
23S:A34
23331
GGGTTCCCAATCCTACACGGATCAACACAAAAAAAATGTGCTAGGAAGT
|
C
|
|
5S:A1
23332
ACTACTGGGATCGAAACGAGACCAGGTATAACCCCCATGCTATGACCGC
|
A
|
|
MM_16S_P10
23333
GCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTG
|
C
|
|
MM_16S_P11
23334
GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAA
|
T
|
|
MM_16S_P12
23335
AGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTT
|
A
|
|
MM_16S_P13
23336
ATTATTCACTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTT
|
T
|
|
MM_16S_P14
23337
CTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATTGAACTTA
|
A
|
|
MM_16S_P15
23338
AACCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATC
|
T
|
|
MM_16S_P7
23339
AATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT
|
T
|
|
MM_16S_P8
23340
TTTATCTTTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAG
|
A
|
|
MM_16S_P9
23341
TTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT
|
A
|
|
RN_16S_P4
23342
AGTGATTGTAGTTGTTTATTCACTATTTAAGGTTTTTTCCTTTTCCTAA
|
A
|
|
RN_16S_P5
23343
TGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAAGCT
|
T
|
|
RN_16S_P6
23344
TTTTTTTAATCTTTCCTTAAAGCACGCCTGTGTTGGGCTAACGAGTTAG
|
G
|
|
RN_16S_P7
23345
TGTTGGGTTAGTACCTATGATTCGATAATTGACAATGGTTATCCGGGTT
|
G
|
|
RN_16S_P8
23346
AGGAGAATTGGTTCTTGTTACTCATATTAACAGTATTTCATCTATGGAT
|
C
|
|
RN_16S_P9
23347
TTTGTGATATAGGAATTTATTGAGGTTTGTGGAATTAGTGTGTGTAAGT
|
A
|
|
MM_28S_P1
23348
GCCGGGGAGTGGGTCTTCCGTACGCCACATTTCCCACGCCGCGACGCGC
|
G
|
|
MM_28S_P10
23349
ACCTCGGGCCCCCGGGCGGGGCCCTTCACCTTCATTGCGCCACGGCGGC
|
T
|
|
MM_28S_P14
23350
TCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGAGTGTCCGGGCCCC
|
C
|
|
MM_28S_P15
23351
CGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGACCCGCCGCCC
|
C
|
|
MM_28S_P16
23352
CGCCGACCCCCGACCCGCCCCCCGACGGGAAGAAGGAGGGGGGAAGAGA
|
G
|
|
MM_28S_P17
23353
GGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAA
|
A
|
|
MM_28S_P18
23354
GGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGGACCCGGCGGGGGAAC
|
A
|
|
MM_28S_P19
23355
CCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGG
|
A
|
|
MM_28S_P2
23356
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCG
|
A
|
|
MM_28S_P20
23357
TGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGCCGGCACCCGCCGGGCT
|
C
|
|
MM_28S_P21
23358
CGAGGCCGGCGTGCCCCGACCCCGACGCGAGGACGGGGCCGGGCGCCGG
|
G
|
|
MM_28S_P22
23359
TCCCCGGAGCGGGTCGCGCCCGCCCGCACGCGCGGGACGGACGCTTGGC
|
G
|
|
MM_28S_P23
23360
TCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTT
|
C
|
|
MM_28S_P24
23361
TCCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGC
|
G
|
|
MM_28S_P25
23362
CCGCCGCGGGGACGACGCGGGGACCCCGCCGAGCGGGGACGGACGGGGA
|
C
|
|
MM_28S_P3
23363
GCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGG
|
G
|
|
MM_28S_P6
23364
CCCACCGGGCCCCGAGAGAGGCGACGGAGGGGGGTGGGAGAGCGGTCGC
|
G
|
|
MM_28S_P7
23365
CCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAGAGGG
|
A
|
|
MM_28S_P8
23366
TATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCGCGGCCGGAGGGC
|
T
|
|
MM_28S_P9
23367
CGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACG
|
G
|
|
RN_28S_P12
23368
GCGCCCCCCCGCACCCGCCCCGTCCCCCCCGCGGACGGGGAAGAAGGGA
|
G
|
|
RN_28S_P14
23369
CGAACCCCGGGAACCCCCGACCCCGCGGAGGGGGAAGGGGGAGGACGAG
|
G
|
|
RN_28S_P16
23370
CACCCGGGGGGGCGACGAGGCGGGGACCCGCCGGACGGGGACGGACGGG
|
G
|
|
RN_28S_P17
23371
GCCAACCGAGGCTCCTTCGGCGCTGCCGTATCGTTCCGCTTGGGCGGAT
|
T
|
|
RN_28S_P4
23372
CCCGGGCCCCCGGACCCCCGAGAGGGACGACGGAGGCGACGGGGGGTGG
|
G
|
|
RN_28S_P5
23373
TGGGAGGGGCGGCCCGGCCCCCGCGACCGCCCCCCTTTCCGCCACCCCA
|
C
|
|
RN_28S_P6
23374
GGGAGAGGCCGGGGGGAGAGCGCGGCGACGGGTATCCGGCTCCCTCGGC
|
C
|
|
RN_28S_P7
23375
CGCTGCTGCCGGGGGGCTGTAACACTCGGGGGGGGGTGGTCCGGCGCCC
|
A
|
|
RN_28S_P8
23376
CGCCGCCGACCCCGTGCGCTCGGCTTCGCTCCCCCCCACCCCGAGAAGG
|
G
|
|
23377
CTCATCCCCACCCTTTTCAACGGATGTGGGTTCGGTCCTCCACTGCCTC
|
T
|
|
23378
AGCCGGGGCTTCTTAGTCAGGTACCGTCATTTTTTCTTCCCTGCTGATA
|
G
|
|
23379
TAGATGATCAACCTACCGGGTTAGAGTAGCCATCACACAAGGGTAGTAT
|
C
|
|
23380
CAGATGGCGGCATTGTCACTGCTCCGTCTCCACGTCACTCCTGAAGGTA
|
G
|
|
23381
GGGAAGCAGGGTGGACCACCACCCAAGGCTAAATACTACCTGATGACCG
|
A
|
|
23382
ACTAAACTTCACTCCGCATCACGTCTTCCCATTGCCGCACGGTTTTTCC
|
A
|
|
23383
GTTCCTCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCACCGTT
|
G
|
|
23384
GCCCCAGACAACCATCGCTGGGGTTGAGCTACCTCACTGCGTCCCTCCG
|
C
|
|
23385
CTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGG
|
A
|
|
23386
CAGGCGTCAGCTCGTATACGTCATCTTTCGATTTAGCACAAACCTGTGT
|
T
|
|
23387
GGCTTCATGCTTAGATGCTTTCAGCACTTATCCCGTCCGCACATAGCTA
|
C
|
|
23388
ATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTA
|
C
|
|
23389
TTCACGCAAGATTTCTCGTGTCCCGCGCTACTCAGGATACCACTACGCT
|
T
|
|
23390
ATCTAAAGTCTTCTCGTTTAAAATACTGGGCTGTTACCATCTGTGGCGG
|
A
|
|
23391
GGGCTCTGACTTCTTGTAGGCATACGGTTTCAGGTTCTCTTTCACTCCG
|
C
|
|
23392
GCTATGGATCGTCGGTTTGGTGGGCCGTTACCCCGCCAACTGCCTAATC
|
C
|
|
23393
ATGACTTCAGCATGGGCGGTCATAACGCGGTACCAGAATATCAACTGGT
|
T
|
|
23394
TTTCAGTTCAGGCGGTTCCCCTCATATACCTATGTATTCAGTATATGAT
|
G
|
|
23395
CGAAAGGGGAGACGGCACGGGCCCGGAGGTTAGCGCCCCAGGCCTCGGT
|
T
|
|
23396
TTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTGTGCC
|
T
|
|
23397
CTCTTATCGATGACATCTCCTCTTAACCTTCCAGCACCGGGCAGGTGTC
|
A
|
|
23398
TCGTCCCTGACAACAGAGCTTTACGATCCGAAAACCTTCTTCACTCACG
|
C
|
|
23399
ACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCA
|
C
|
|
23400
GTCCTCTCGTACTAAGGACAGAGCTCCTCAAATATCCTGCGCCCACGAC
|
A
|
|
23401
TTATAGTTACGGCCGCCGTTTACCGGGGCTTCAATTCAGAGCTCTCACT
|
C
|
|
23402
CGTTTCTACGAGTTAGAACTCAAATAATCAAAGGGCCGTATTTCAACAG
|
C
|
|
23403
CACCAGTGTCGGTTTAGGGTACGGGCGGACCCGCCACCTCGCTCACGAA
|
G
|
|
23404
CGTCCATCCCGGTCCTCTCGTACTAGGGACAGCTCCTCTCAAATATCCT
|
G
|
|
23405
AGCTGACGCTCATGTTTCCAAGTCTCCCGCCTATCCTGTACATAGATTT
|
C
|
|
23406
CTCTTTTAATGAGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGC
|
A
|
|
23407
ACAGCTTTTCTCGCCATCTTCCATCCCAGACTTCGGTACTAACTTCCCT
|
C
|
|
23408
CATAGACCTGTGTTTTTGCTAAACAGTTGCTTGAGCCTATTCTCTGCGG
|
C
|
|
23409
TCACGGTACTGGTTCACTATCGCTCACTCGTTTATATTTAGCCTTGGCG
|
G
|
|
23410
ACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCCGGC
|
G
|
|
23411
GGCTACAGTAAAGCTCCATGGGGTCTTTCCGTCTTGTCGCGGGTAACCG
|
G
|
|
23412
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGG
|
C
|
|
23413
AAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCGGTAGGGGC
|
C
|
|
23414
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAG
|
T
|
|
23415
CCCTTCTCCCGTTGGCCTTAGAATCTTCTTCCTACCTACCTGTGTCGGT
|
T
|
|
23416
TACCTTCACTAAGGTTCTTTCCGACGCTAGCCCTAAAGCTATTTCGGGG
|
A
|
|
23417
CCCCCCTGCTTCCCACAGGGTTTCACGTGTCCCGTGGTACTCTGGATCA
|
C
|
|
23418
GACCGGCCTTCCCATGCCGTTCGGTTAACAGATTAAGTCTTAAAAGCAG
|
T
|
|
23419
TTCCTTTGACCCCCCCCCCCCCCCTCCCTATCCCCCCCCGCCCCCCCCC
|
A
|
|
23420
CCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCA
|
C
|
|
23421
CTTTGGGAGGCAACCGCCCCAGTTAAACTACCCGCCAGGCACTCTCCCC
|
G
|
|
23422
ACATGATCGGTTCACACACTCACCACCACACAAGACCTCAAAGAGACCC
|
C
|
|
23423
CCAGCACCGGGCAGGTGTCACCCCCTATACTTCGTCTTGCGACTTCGCA
|
G
|
|
23424
GTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACTGACTACAGCC
|
C
|
|
23425
CCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGT
|
G
|
|
23426
TTCTCTGCGGCTCATGTTTCCATGAGCACCCCTTATCCCTAAGTTACGG
|
G
|
|
23427
TTTGACTCATATCACACCTCACTGCTTAGACGTGCACTTCCAATCGCAC
|
G
|
|
23428
CCGGTTTGCCCTCTTCCGCGTTCGCTCGCCACTACTTACGGAATCTCGT
|
T
|
|
23429
TACCTGATCGACTTGTCAGTCTCCCAGTCAAGCGCCCTTATGCCATTAC
|
A
|
|
23430
TCCCAAGCTTCGGTGTATGATTTAGCCCCGTTAAATTTTCGGCGCAGGG
|
T
|
|
23431
CCTAGTCTTTTCAGTGCTCTACAAGCCGTGGTCATGGTTCGAGGCTGTA
|
C
|
|
23432
TCGGGGTGCTTTTCACCTTTCCTTCACAGTACTCGTACGCTATCGGTCT
|
C
|
|
23433
GGTCTGGGCTCTTTCCCTTTCGACTGCCCAACTTATCTCGTGCAGTCTG
|
A
|
|
23434
GCACTCCACAGCTCCTTCCGGTACTGCTTCTTCGCGTTAAGAATGCTCC
|
T
|
|
23435
GACTGCGAACCGTGAGCATTCGGAGTTCGTCAGGACTCGATAGGCGGTG
|
A
|
|
23436
GTAAACAGTCGCTTGGGTCTATTCTCTGCGGCCCATTCCTGGGCACTCC
|
T
|
|
23437
CCCACTTTCGTGCCTGCTCGACGTGTCTGTCTCGCAGTCAAGCCACCTT
|
G
|
|
23438
TTTCCCTGCGGCTCCGGGACTTTATCCCTTAACCTTGCCAGTATGCACA
|
A
|
|
23439
GGGCGCCTTCGCTTCGTAGCAGCTTTTCTCGCCAGCGTGAATTCAGCAG
|
C
|
|
23440
TTCCGCCTGACCTTAGCTCCCGACTAACCCTGAGCGGACGAACCTTCCT
|
C
|
|
23441
CTCTCAGGTCGGCTACTGATCGTCGGCTTGGTAGGCCGTTACCCCACCA
|
A
|
|
23442
CTTCCTCCGGCTACTTAGATGTTTCAGTTCACCGGGTTCCCCTCCATAC
|
G
|
|
23443
TACCTGATCGACTTGTTAGTCTCCCAGTCAAGCGCCCTTATGCCATTAC
|
A
|
|
23444
GCAACCGCCCCAGTTAAACTACCCGCCAGGCACTGTCCCTGAACAGGAT
|
G
|
|
23445
TTCCTCGTGTCTCGCCGTACTCAGGATCCCATTAGGCTTCGATCGGATT
|
T
|
|
23446
ACGGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATGCAC
|
C
|
|
23447
TGTCGGTTTGGGGTACGGGCGGCAACGCGCCTGACGCCGGGGCTTTTCT
|
C
|
|
23448
CGGTTTCCGTTCGCGCTGAGGGAACCTTTGGGCGCCTCCGTTACATTTT
|
G
|
|
23449
TTATAGTTACGGCCGCCGTTTACTGGGGCTTCAATTCAATGCTCTCACA
|
T
|
|
23450
TGTAGCATGCGTGAAGCCCTGGACGTAAGGGGCATGATGATCTGACGTC
|
A
|
|
23451
AGCACCGGGCAGGTGTCAGCACCTATACGTCAGCTCTCGCTTTCGCAGA
|
T
|
|
23452
GCTGATAGGACGCGACCCCATCCCACGCCGATAGAATCTTTCCCACAAT
|
C
|
|
23453
GTTTCAGGTTCTATTTCACTCCCCTCCCGGGGTGCTTTTCACCTTTCCC
|
T
|
|
23454
CGGCTCCCATTCCGTGTCACCCCTGCGCTCACCTACCACGGCTACGCTC
|
C
|
|
23455
TAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTATATTT
|
C
|
|
23456
GGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGT
|
T
|
|
23457
CATACCAGAGGTTCGTCCACCCAGGTCCTCTCGTACTATGGGCAGGCCT
|
C
|
|
23458
CGCGGGTCCATCTTATACCACCGGAGTTTTTCACACTGAGCCATGCAGC
|
T
|
|
23459
CTCCCGCAACCCCGGCCACGCAACCCCCGACGGGTATCGCGCGCGGCCG
|
G
|
|
23460
TTCTCTGCGGCTCCATCTCTGGAGCACCCCTTCTCCCGAAGTTACGGGG
|
T
|
|
23461
GAACATCCGGCATTACCACCCGTTTCCAGGAGCTATTCCGGAGCATGGG
|
G
|
|
23462
AGGTCCCGGGGTCTTTTCGTCCTTCTGCGCTTAACGAGCATCTTTACTC
|
G
|
|
23463
GCTTCGGTGGCATGTTTTAGCCCCGGACATTTTCGGCGCAGGACCTCTC
|
G
|
|
23464
GCTTCAAAGCCTCCGACCTATCCTACACATCACGTGCCCAGATTCAATG
|
A
|
|
23465
TACTTTATTTCGCTCCACATCACGGCTTCGTCTCATGCACAGCGGATTT
|
G
|
|
23466
CATGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTAC
|
T
|
|
23467
GACCTTCCTCTCAGAACCCCTACTGATCGTTGCCTTGGTGGGCCGTTAC
|
C
|
|
23468
ATGTTTCAGTTCCCCGGGTTCCCCTCCATACGTTATGGATTGGCGTATG
|
G
|
|
23469
TTAACGCTTTCGCTTGGCCGCTTACTGTATATCGCAAACAGCGAGTATT
|
C
|
|
23470
CCACGGAAAACCACCTCCGCGGCCGGCTCCCATTCCGTGTCACCCCTGC
|
G
|
|
23471
TCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCTCTCACCCATTAAC
|
G
|
|
23472
AGGATGCGACGAGCCGACATCGAGGTGCCAAACCTCCCCGCCGATATGG
|
A
|
|
23473
TCCCCGGAGTACCTTTTATCCTTTGAGCGATGTCCCTTCCATACGGAAA
|
C
|
|
23474
CGGCTTCCCTACTTTAATTTCGGTCCCTTACGCCCGGGTCAACCAACGC
|
C
|
|
23475
CTGCTTCCAAGCCAACATCCTAGCTGTCTTAGCAGTCAGACTTCGTTAG
|
T
|
|
23476
GCTACTCATACCGGCATTCTCACTTCTATGCGTTCCAGCGCTCCTCACG
|
G
|
|
23477
GCCTTCGGTGTCTGCCTTATACCCGATTATTATCCATGCCCGGACCCTC
|
G
|
|
23478
CCGGCTTTCCCAAAACCGTTCCACTAACATTGCAGAATCTTAAATGCAG
|
T
|
|
23479
TACCTGTGTCGGTTTGCGGTACGGGCACCTTAGTATACACATAAGCTTT
|
T
|
|
23480
TGTTACGCACTCTTTCAAGGGTGGCTGCTTCTGAGCCAACCTCCTGGCT
|
G
|
|
23481
CTGGAGACCTTGGATATTCGGCCACAAGGATTCTCACCTTGTTCTCGCT
|
A
|
|
23482
CAGTAACCCGCAAGGCTGCACCTAAATGCATTTCGGGGAGTACGAGCTA
|
T
|
|
23483
AAACCTTGGATATTCGGCCTAGAGGATTCTCACCTCTATCTCGCTACTC
|
A
|
|
23484
CGCTTGTGCGGGCCCCCGTCAATTTCTTTGAGTTTTAGCCTTGCGACCG
|
T
|
|
23485
ACCGGGACACGTGATCCCACAACACCGGCAACGCAACCCCCGACGGGTA
|
T
|
|
23486
GCTTTTCTCGCCTTCAGCCAAGTGTGCTTCCCTACTCTAATTTCGGTCC
|
C
|
|
23487
CACTACTCACGGAGTATCCCTTCCTGCAGGTACTGAGATGTTTCACTTC
|
C
|
|
23488
GATTGGAATTTCTCCGCTACCCACAGTTCATCCGCTACCATTTCAACGG
|
G
|
|
23489
TTCCACGAGTCCCGCGCTACTCGGGAGACACCATCCATGGTGCACGCGC
|
A
|
|
23490
GTCTTTTCGTCCCATCGCGGGTAATCGGCATCTTCACCGATACTACAAT
|
T
|
|
23491
CCGTACATCATCTCGATGGCATTCGGAGTTTGATATTCTTTGGTAAGCT
|
T
|
|
23492
GGGCTTGGCTACCCGGCTATAGACTTGGCAGTCTAACCGGTGCACCAGC
|
G
|
|
23493
ACTTTCGTTACTGCTCGACCCGTCAGTCTCGCAGTTAGGCTCGCTTCTG
|
C
|
|
23494
CTACTGTTTCTCCGCGTATACAACGCTCCCCTACCCAATCCATTACTGG
|
A
|
|
23495
ACTTATAGTCAGCGCCCCTTCTCCCGAAGTTACGGGGCCATTTTGCCGA
|
G
|
|
23496
CTTCCAAGCCAACATCCTAGCTGTCTTAGCAATCTGACTTCGTTAGTTC
|
A
|
|
23497
CCTCGGCAACTGGCGTTACCGATTCTCAGCCTCCCACCTATCCTGTACA
|
T
|
|
23498
CCATAACGGCTCCCATCATCACACCTCGCCATGCATGCCATGCGGATTT
|
G
|
|
23499
CGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG
|
T
|
|
23500
CATCCAAACACTTTTCAACGTGTCCTGGTTCGGTCCTCCAGTGCGTTTT
|
A
|
|
23501
GCCCTAAAGCTATTTCGGGGAGAACCAGCTATATCCGGGTTCGATTGGA
|
A
|
|
23502
CAGTAAAGCTCTACGGGGTCTCTCCGTCCAGTCGCGGGTAATGGGCATC
|
T
|
|
23503
GGAACCTTTGGGCGCCTCCGTTACGCTTTAGGAGGCGACCGCCCCAGTC
|
A
|
|
23504
CCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAGTTTGCA
|
T
|
|
23505
CAGGTGTCAGCCCCTATACTTCATCTTTCGATTTGGCAGAGACCTGTGT
|
T
|
|
23506
GACTCTTCCCAGAGTCTTCTTCTATTCCCTTGGCTGCTTTATCGCAGTC
|
C
|
|
23507
GGCAACCCAACAACCCACACACCATCATCTTCAGCTACAGGACTATCAC
|
C
|
|
23508
AGCACCGGGCAGGTGTCAGGCTATATACCTCATGTTTCCATTTCGCATA
|
G
|
|
23509
TTGCATACTATTAAGTTCAGCTCGGAAGGTGGATTTGCCTGCCTTCCTC
|
A
|
|
23510
CCGGCGGATTTGCCAACCGGACACCCTACACCCTTGGACCAGGTCAATT
|
C
|
|
23511
GCCGGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAG
|
C
|
|
23512
CTGATACAACCAGTATCGCTCCGTCCATTTGCGCAGCACCAGTAATCAT
|
G
|
|
23513
TCTTTGAATGTATGGCTGCTTCTGAGCCAACATCCTAGTTGTCTTCGAG
|
A
|
|
23514
TGGATTCTCGCCCTCTTGTACTCATTTCGACTACGGGACTGTTACCCTC
|
T
|
|
23515
CAGTATCAACTGCAATTTTACGGTTGAGCCGCAAACTTTCACAACTGAC
|
T
|
|
23516
TTCTCTGCGGCTTACCTTCGTAAGCACCCCTTCTCCCGAAGTTACGGGG
|
T
|
|
23517
ATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCG
|
A
|
|
23518
CATAGACCTGTGTTTTTGCTAAACAGTTGCTTGAGCCTATTCTCTGCGG
|
C
|
|
23519
TATAAGTCGAGGCTGCACCTAAATGCATTTCGGGGAGTACGAGCTATCT
|
C
|
|
23520
TCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTAGGTCCCG
|
A
|
|
23521
GGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCG
|
G
|
|
23522
ATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT
|
T
|
|
23523
CCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCCT
|
T
|
|
23524
AAAAAGCAAGCTCTCTCAAGTTCCGTTCGACTTGCATGTGTTAGGCGCG
|
C
|
|
23525
GGGCCCGTGTCTCAGTGCCCATGTGGGGGACCCTCCTCAGGCCGGCTAT
|
C
|
|
23526
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAA
|
C
|
|
23527
CAACCTGTTGTCCATCGGCTACGCTTTTCAGCCTCACCTTAGGTCCCGA
|
C
|
|
23528
CACACACCACCACCACCCGAAAGCGGAGGCGGGGCGCGGGCAGATTGGT
|
T
|
|
23529
CCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCATCCTGAGCC
|
A
|
|
23530
GGCACCCTCTACGGCCAGGCCTTCAAGCCTGTTCCCCTGGCAAGCCGTT
|
T
|
|
23531
GCCCTTCAAAAGCGTCCCTGTGTTTAAATCTTCGGAGGTTACGGAATTT
|
C
|
|
23532
TCGTGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGC
|
G
|
|
23533
TCCCGGGGTTCTTTTCACCGTTCCTTCACAGTACTATGCGCTATCGGTC
|
A
|
|
23534
GACTGTTCGAGGTTAGACATCAAACGAGAACAGAGCGGTATTTCACCTT
|
G
|
|
23535
CACCTTAGAGTGCCCAACTGAATGCTGGCAACTAAGATCAAGGGTTGCG
|
C
|
|
23536
TATGGCACTTAAGCCGACACCTCACGGCACGAGCTGACGACAACCATGC
|
A
|
|
23537
TCTCGTCCATTGACCAATATTCCTCACTGCTGCCTCCCGTAGGAGTTTG
|
G
|
|
23538
TTTTCACCTTTCCCTCACGGTACTGGTTCGCTATCGGTCTCTCGGGAGT
|
A
|
|
23539
TTCCCCATTCAGAGATCTCCGGATCAATGGATATTTGCTCCTCCCCGAA
|
G
|
|
23540
TGAGCCAACATCCTGGTTGTCTGCGTATCTTCACATCGTTTTCCACTTA
|
A
|
|
23541
TCGGAGTTTGATATTCTTCGGTAGGCTTTGACGCCCCCTAGGAAATTCA
|
G
|
|
23542
CCTTCGGCTCCCCTATTCGGTTAACCTTGCTACAGAATATAAGTCGCTG
|
A
|
|
23543
GTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCA
|
G
|
|
23544
TTATCCGTTCCGTACATAGCTGCCCAGCCGTGCCATTGGCATGACAACT
|
G
|
|
23545
TTCACAGTACTATGCGCTATCGGTCACTAAGGAGTATTTAGCCTTGCGG
|
G
|
|
23546
GACTCACCCGGGGACGACGAACGTGGCCCCGGAACCCTTGGTCATCCAG
|
C
|
|
23547
GGCAACTTCAACCTGCACATGGATAGATCACCCGGTTTCGGGTCTACGT
|
A
|
|
23548
ACCACGAATTCCGCCTGCCTCAACTGCACTCAAGATATCCAGTATCAAC
|
T
|
|
23549
ACCACGCATTGCTGCATCCCAAGCTTCGGTTACATGCTTAGCCCCGTTA
|
C
|
|
23550
CCAGAGCTTTTCTCGCCTCCGTCCAAGCATGCTTCCCTACTAAATTTCA
|
G
|
|
23551
GCTGCACCTAAATGCATTTCGGAGAGAACCAGCTATCACGGAATTTGAT
|
T
|
|
23552
CCTGGTTCGGGCCTCCAGTGAGTTTTACCTCACCTTCACCCTGCTCATG
|
G
|
|
23553
ACTCACCCGGGGACGACGAACGTGGCCCCGGAACCCTTGGTCATCCAGC
|
G
|
|
23554
AACATCCTGGTTGTCTGTGCAATTCCACATCCTTCTCCACTTAACGTGA
|
A
|
|
23555
CTACGACTTCTCCCCATACAGAACGCTCTCCTACCATACATTAGATGTA
|
T
|
|
23556
CACACTTAGCCCCGGACAACCATCACCGGGGATGAGCTACCTCACTGCG
|
T
|
|
23557
GGGCGACCCTCCAACAGCGGCGGAACACATTTCGACTACGGGACTCTCA
|
C
|
|
23558
CTCCGGTGCTTAACCTTGCCAGTGAGCGCAACTCGCCGGACCGTTCTAC
|
A
|
|
23559
TTCGCAGGCTTACAGAACGCTCCCCTACCCAACAACGCATAAGCGTCGC
|
T
|
|
23560
CCGTCAAGCCATGGGAGCCGGGTGTACCTAAAGTCGGTAACCGCAAGGA
|
G
|
|
23561
TTACCTACACCATCACCTACACGCTTACACCAACAATCCACTAAGCGGC
|
A
|
|
23562
GCGTACACCTGCAGCCTATCTACCTCGTAGTCTTCAAGGGGTCTTACCT
|
G
|
|
23563
GCCGTCGCCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCAC
|
C
|
|
23564
CACAGTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGA
|
C
|
|
23565
CTGTTATCCCCAGGGTAGCTTTTATCCGTTGAGCGACGGCATTTCCACT
|
C
|
|
23566
ACTTAGATGCTTTCAGCACTTATCCAATCCCGACTTAGATACCCGGCAA
|
T
|
|
23567
GCTTGCGCTAACCTCTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAG
|
C
|
|
23568
ACCTATCCTGTACATGTGGTACAGATACTCAATATCAAACTGCAGTAAA
|
G
|
|
23569
CTCCACCAGACTAAAACGAGGCTAGCCCTAAAGCTATTTCGAGGAGAAC
|
C
|
|
23570
CCCGGCTTACCTTGGGCGGACGAACCTTCCCCAAGAAACCTTAGATTTT
|
C
|
|
23571
GCAGAACAACTGGTACACCAGCGGTGCGTCCATCCCGGTCCTCTCGTAC
|
T
|
|
23572
GACCAGGTCGATTCCATTGCCTGGCCCGGCTACCTTCCTGCGTCACACC
|
T
|
|
23573
CTCTGAGACTTCAAATGTGTCCCTGTGCTTAACTCTTTTGGTGGTGACG
|
G
|
|
23574
ACCTCGCGGTACGCCTTCGACGCTGACTGGAATGCTCCCCTACCGATCA
|
T
|
|
23575
CGTCCATCCTGAGGGAACCTTTGGGCGCCTCCGATACCCTTTCGGAGGC
|
G
|
|
23576
CACCTATCGGTCTCTCCTTAGGTCCCGACTAACCCAGGGCGGACGAGCC
|
T
|
|
23577
CGCTCGCCGCTACTAAGGAAATCGATGTTTCTTTCTCTTCCTCCGGCTA
|
C
|
|
23578
CGCGAGTCCATCTTCAAGCGATAAAATCTTTGATATCAAAACCATGTGG
|
T
|
|
23579
TGACTGGAGTTTGTCCAGCCGGGTTTCCCCATTCAGAGATCTGCGGATC
|
A
|
|
23580
CCTACTTAGCTACCCGGCTATGCCCCTGGCGGAACAACCGGTGCACCAG
|
C
|
|
23581
ACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCCGTC
|
C
|
|
23582
GATTTGCCTGGGATAATCAACATCTACACCCTTTAACGGACTATTCCGT
|
C
|
|
23583
CTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTA
|
T
|
|
23584
GGATCTTAGCACTCGCAGTCTGACTGCCGACCATAAATCAATGGCATTC
|
G
|
|
23585
ACCTATCCTGTACATGTGGTACAGGTACTCAATATCAAACTGCAGTAAA
|
G
|
|
23586
TCACCGGGGATGAGCTACCTCACTGCGTCCCTCCGCAGCTTGCCTACTA
|
C
|
|
23587
GCCATGCAGATTCTCACTGCATTCGCGCTACTCATTCCGGCATTCTCAC
|
T
|
|
23588
CTTCACCTCACATACGACGCTCCCCTACCCCTGACAATTACTTGTCAAG
|
C
|
|
23589
CCCTACTGATCGTCGCCTTGGTGGGCCGTTACCCCGCCAACAAGCTAAT
|
C
|
|
23590
ACGCATTCGGAGTTTGTCAAGACTTGATAGGCGGTGAAGCCCTCGCATC
|
T
|
|
23591
ACATTTTAGGAGGCGACCGCCCCAGTCAAACTGCCCGTCAGACACTGTC
|
T
|
|
23592
GGTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTTGCTTACAGCT
|
C
|
|
23593
CTCATCCCCACCCTTTTCAACGGATGTGGGTTCGGTCCTCCATTGCCTT
|
T
|
|
23594
AGGTCACTTGGTTTCGGGTCTACATCTACGTACTTAACCGCCCTTTTCA
|
G
|
|
23595
ACACACTCACCACACCACCACAACATCAAAGACATCACAATGGCAGGCT
|
C
|
|
23596
TGACAACTGGTGCACCAGAGGTGCGTCCATCCCGGTCCTCTCGTACTAG
|
G
|
|
23597
TCTGCCTCTGCACATTGCTCCTCTACCGCGCATCTTCTTCAGACGCACC
|
C
|
|
23598
CTTTTCTCGACAGTACGGGATCACCAACTTCACCAATTAAGGCTACGCA
|
T
|
|
23599
CCCTCATGTCACTATTTATTCATGACATGATGACACGCTGTTAACGTGC
|
C
|
|
23600
GTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGG
|
T
|
|
23601
GGCGACCACCCCAGTCAAACTACCCACCAAGCAATGTCCGCGCATAGCG
|
C
|
|
23602
GACTTAGTCCCAATCACGAGCCTCACCTTAGACGGCTCCATCCCACAAG
|
G
|
|
23603
GCGCTTATGCGGTATTAGCAGTCATTTCTAACTGTTATCCCCCTGTATA
|
A
|
|
23604
CGCTTTCACTGCGGCTACGTGTCTCGTGACACTCAACCTCGCCAGTGAC
|
G
|
|
23605
ATGCTTTTCGCTTACAGGACTATAACCTTCTTTGGTGTGCCTTCCCATA
|
C
|
|
23606
CGACTAACCCAGGGCGGACGAGCCTTCCCCTGGAAACCTTAGTCTTACG
|
G
|
|
23607
TAGGACCCGACTAACCCTGATCCGATTAGCGTTGATCAGGAAACCTTAG
|
T
|
|
23608
ACAGCTTTTCTCGTCTCTTTCCAAACTGACTTCCGCTTACGCGTCCCTT
|
A
|
|
23609
TAAGACTTGCTCTCGCTGCGGCTTCAGACCTTAAGTCCTTAACCTTGCC
|
A
|
|
23610
CTCTCAAACCAGCTATGGATCGTCGGCTTGGTAGGCCATTACCCCACCA
|
A
|
|
23611
GGAATTTCTCCCCTATCCACACGTCATCTCCACCCTTTTCAACGGATGT
|
G
|
|
23612
CCGGTCCATGGTCGGTACGGGAATATCCACCCGTTCATCCATTCGACTA
|
C
|
|
23613
CCCCCGACCGGTTTCACGGCCGCAGGTTAGAATTCCAGAAACCTAAGGG
|
C
|
|
23614
AAGTTTCGGTGGCTACGGAATTTCAACCGTATGTGCATCGACTACGCCT
|
C
|
|
23615
TGCGCTCCCTTTACACCCAGTAAATCCGGATAACGCTTGCCCCCTACGT
|
A
|
|
23616
ATTTCGCCTACGGGACTGTCACCCTCTATGGTCCACCTTTCCAGGTGAG
|
T
|
|
23617
GCTTCGGTGGCATGTTTTAGCCCCGGACATTTTCGGCGCAGGACCTCTC
|
G
|
|
23618
GACATGTCTCCACATCATTCAGTTGCAATTCAAGCCCGGGTAAGGTTCC
|
T
|
|
23619
CGATAACTGGCACACCAGAGGTGCGTCCTTCCCGGTCCTCTCGTACTAG
|
G
|
|
23620
AACGCTTATCGGTGCGGACCTCCATCCCGTGTTACCGGGACTTCATCCT
|
G
|
|
23621
CCACTCCGTCGATGTGAACTCTTGGGAGTGATAAGCCTGTTATCCCCAG
|
G
|
|
23622
GCCGCCTTTTCAACGGAGGTCGGTTCGGCCCTCCATGGAGTTTTACCTC
|
C
|
|
23623
ACCGTTATAGTTACGGCCGCCGTTTACTGGGGCTTCAATTCGCACCTTC
|
G
|
|
23624
AGGTGTTCTCATGTGGGTTTCCCCATTCAGAGATCTGCGGGTCAATGGA
|
T
|
|
23625
AGCCTGTTCCCCTGGCAAGCCGTTTTATGACTCCCGCCCGGTCCGTCGG
|
A
|
|
23626
GCTGACCTACTACGAGGGGGGATCCCAACGCGCCCGCGCCGCGACCCCC
|
C
|
|
23627
GTTATCCCCCTGTATGAGGCAGGTTACCCACGCGTTACTCACCCGTCCG
|
C
|
|
23628
CGGACATCTTCGGCGCACAATCACTCGACCAGTGAGCTATTACGCACTC
|
T
|
|
23629
TGCTTGATGCCCGATTATTATCCACGCCAAACTCCTCGACTAGTGAGCT
|
G
|
|
23630
CTCCATTCGGAAATCTGCGGATCAAAGCCTACTTACGGCTCCCCGCAGC
|
T
|
|
23631
GCTGTTGGTCCGGATTGTTCTCCTTTAGGACATGGACCTTAGCACCCAT
|
G
|
|
23632
TGCTGGCACGGAGTTAGCCGTCACTTCCTTGTTGAGTACCGTCATTATC
|
T
|
|
23633
GCTATCGGTCAGACAGGTATGCTTAGACTTACCCAACGGTCTGGGCTGA
|
T
|
|
23634
TATTCCTCACTGCTGCCTCCCGTAGGAGTTTGGACCGTGTCTCAGTTCC
|
A
|
|
23635
TCCCGCTGGCCTTAGAATTCTCTTCCTGTCCACCTGTGTCGGTTTGCGG
|
T
|
|
23636
CGACTATTGTCCTCGGCTTAGGTCCCGACTTACCCTGAGAGGACGAGCC
|
T
|
|
23637
GGTCCTTTTCACCTTTCCTTCACAGTACTATGCGCTATCGGTCACTAAG
|
T
|
|
23638
TCGGCTACTGATCGTCGCCTTGGTAGGCCGTTGCCCTGCCAACTAGCTA
|
A
|
|
23639
CTTGGGAGTATGTTTACACGCACTATTACCGTTTTCCGAGGAAATTGGT
|
A
|
|
23640
CACACAACCCCTACCAGGTATCACATGCACACGGTTTAGCCTCATCCAC
|
G
|
|
23641
CCACGGCTTCGGTGTTGTGTTTTAGCCCCGGACATTTTCGGCGCAGGGC
|
C
|
|
23642
CCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCG
|
G
|
|
23643
AGCTTTCGGGGAGAACCAGCTATCTCCCGGTTTGATTGGCCTTTCACCC
|
C
|
|
23644
CGAGCCTTCCTCAGGAAACCTTAGGCATTCGGTGGAGGGGATTCTCACC
|
C
|
|
23645
CCCAGGGCTAGATCATCCCGCTTCGGGTCCAGGACAAGCGACTGAAAAC
|
G
|
|
23646
AAAATCATGGGAAATCTCATCTTGAGGGGGGCTTCGCACTTAGATGCTT
|
T
|
|
23647
ATCCTGTACAAGCTGTACCAACATTCAATATCAGGCTGCAGTAAAGCTC
|
C
|
|
23648
TTAGCAGGTGGTCCGGATTCTTCTCCTCTCGGGCACGGACCTTAGCACC
|
C
|
|
23649
GTCCGTTTACGGTACGGGTACCTCAAGGATAAGTTTAGCGGGTTTTCTA
|
G
|
|
23650
CACTGGCGTGCTGCCTTCTCTGCCTCCCACCTATCCTGTACATGAAATA
|
C
|
|
23651
TGCGGTATTAGCAGTCATTTCTAACTGTTATCCCCCTGTATAAGGCAGG
|
T
|
|
23652
GCTATCGGTCAGACAGGTATGCTTAGACTTACACCACGGTCGGTGCGGA
|
T
|
|
23653
TTTACTCCTTTCGGATGGGATATCTCATCTTGAGGGGGGCTTCACGCTT
|
A
|
|
23654
TGGCCGGTCGCCCTCTCAGGCCGGCTACCCGTCGAAGCCTTGGTGAGCC
|
G
|
|
23655
AAGCCTGTTCCCCTGGCAAGCCGTTTTATGACTCCCGCCCGGCCCGTCG
|
G
|
|
23656
AAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCT
|
G
|
|
23657
GACATCATACTAACGCGCCCTATTAAGACTCGGTTTCCCTACGGCTCCG
|
T
|
|
23658
TGTGTTTTTGTTAAACAGTTGCCTGGACCGATTCTCTGCGCCTCAAGTC
|
G
|
|
23659
GCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG
|
T
|
|
23660
GCGTCACACCTGTTAATGCGCTTGCCTTACCGGTTCAGGTCCCGCGCTC
|
C
|
|
23661
GCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCG
|
T
|
|
23662
AAGCTCCATGGGGTCTTTCCGTCTAGTCGCGGGTAACCGGCATCTTCAC
|
C
|
|
23663
CGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGTTCGT
|
T
|
|
23664
TCCCATCCGCACTTCGCTTCCCTGCTATGCCGTTGGCACGACAACAGTT
|
G
|
|
23665
TTTCACTCCCCTCCCGGGGTCCTTTTCACCTTTCCTTCACAGTACTCTG
|
C
|
|
23666
CGTCCTCGGCTTAGGCCCCGACTTACCCTGGGCGGATGAACCTTCCCCA
|
G
|
|
23667
CGACATCGAGGTGCCAAACCTCCCCGTCGATGTGGACTCTTGGGGGAGA
|
T
|
|
23668
TACCTGATCGACTTGTCAGTCTCCCAGTCAAGCGCCCTTATGCCATTAC
|
A
|
|
23669
CTTCCAAGCCAACATCCTAGCTGTCTTAGCAATCTGACTTCGTTAGTTC
|
A
|
|
23670
ACGCCTTAACCATGTGAAGGGTAGATTTTCTGACCCCTTCGGCCTGAAC
|
G
|
|
23671
CTCAAGGATTAAGTTTAGCGGATTTTCTCGGGAGTATGTTTACACGCAC
|
T
|
|
23672
CCCCATCCATCACCGATAAATCTTTAATCTCTTTCAGATGTCTTCTAGA
|
G
|
|
23673
ATACTTTGGGACCTTAGCTGTGGGTCTGGGCTGTTTCCCTTTTGACAAT
|
G
|
|
23674
CGCCCATAGGCGGTGCCGGCCCATGACGGCCGGCGGGTTCCCCCATTCG
|
G
|
|
23675
AAAATCATGGGAAATCTCATCTTGAGGTGGGCTTCGCACTTAGATGCTT
|
T
|
|
23676
ACAACTTGATACCCGATTATTATCCACGCCCGACTCCTCGACTAGTGAG
|
C
|
|
23677
CTGAGTTTGATAAGCTTCGCTAACCTCTCGGCCGCTAGGCTATTCAGTG
|
C
|
|
23678
GCCCAGATCGTTGCGCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGA
|
A
|
|
23679
TTATAGTTACGGCCGCCGTTCACTGGGGCTTCGGATCACTGCTTCAGAT
|
C
|
|
23680
GGCATTGTCCCACCGCCGGGTCACGGCGGCTGGTTAGAAACCCAATACT
|
G
|
|
23681
GTCCACACATTTAGCCCCAGACAACCATCGCTGGGGTTGAGCTACCTCA
|
C
|
|
23682
TCTCACGACGTTCTGAACCCAGCTCGCGTGCCGCTTTAATGGGCGAACA
|
G
|
|
23683
ATGCGACGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGAACT
|
C
|
|
23684
CCTGTGTCGGTTTAGGGTACGGGCAGTTTGAACCTCGCGCCGATGCTTT
|
T
|
|
23685
CGATATTGCAAGGGTGGTATCCCAACAGCGCCTCCTCAGAGACTGGCGT
|
C
|
|
23686
CCCCCGACCGGATTCACGGCCGCAGGTTAGAATTTCAGCACCTCAAGAG
|
T
|
|
23687
TCAGATGGCGGCATTGTCACTACTGCGTCTCCACATCACTCCTGGAGGT
|
A
|
|
23688
CTTTTCGTCCCATCGCGGGTAATCGGCATCTTCACCGATACTACAATTT
|
C
|
|
23689
ACAACGAATTCCGCCAACTTCCCGCGCACTCAAGCCCTCCAGTTCGCGC
|
T
|
|
23690
CCCGAAGTTACGGGGCCAATTTGCCGAGTTCCTTAACAACCCTTCTCCC
|
G
|
|
23691
TCAAGGGGGTTTACTTCTTTCGAATGGGATATCTCATCTTAAGGGGGGC
|
T
|
|
23692
CTTCACAGTACTATACGCTATCGGTCACTGGGTAGTATTTAGGGTTGGA
|
G
|
|
23693
ATTCCGTCAGACGGCCGGACTGTCACTTCTCCGTCACCACATCGCTCTC
|
T
|
|
23694
CGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGGTTGGGAGAT
|
G
|
|
23695
AGCTGATGGTCCGGATTCTTCTCCTTTAGGACATGGACCTTAGCACCCA
|
T
|
|
23696
CGTATTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCATAAG
|
G
|
|
23697
ACGGGTTAGCCTCGCCACGCACCACTGACTCGCAGACTCATTTTTCGAT
|
A
|
|
23698
ACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTC
|
G
|
|
23699
TGCGCATTCGGAGTTTATCAAGACTTGATAGGCGGTGAAGCCCTCGCAT
|
C
|
|
23700
CTGTTGTCCATCGGCTACGACTCTCGTCCTCACCTTAGGCCCCGACTTA
|
C
|
|
23701
GGCTCACGCCTCACCTTCGACGCGGAGTGGAATGCTCCCCTACCGATGT
|
T
|
|
23702
GATGTTTCAGTTCAGGCGGTTCCCTCGATATACCTATTTTTAAGTTCAG
|
T
|
|
23703
CATTGTCTAAGATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTG
|
T
|
|
23704
TCACAGTACTATGCGCTATCGGTCACTAAGTGGTATTTAGCCTTAGGGG
|
G
|
|
23705
GTAGTATTTAGGCTTGGAGGATGGTCCCTCCTGCTTCCCACAGGGTTTC
|
A
|
|
23706
TTGGGACCTTAGCTGCGGGTCTGGGCTCTTTCCCTTTTGACTATCCAAC
|
T
|
|
23707
CAGCTTGGTGGCGCAGAACTAAGCATTTGACTCAGTCCTCACCTCACTG
|
C
|
|
23708
ACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGC
|
C
|
|
23709
AAGCCCGCTTGTGCGATTACACTCGACACCCGATTGCCAACCGGGCCGA
|
G
|
|
23710
CCTTAAATACGCACAACCATCGGCGCACTGCAGCTACCTGTCTGCGTCA
|
C
|
|
23711
CTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTAAGTC
|
C
|
|
23712
CCTGTGTCGGTTTACGGTACGGGCGCATGGCAAACAATAGCGGCTTTTC
|
T
|
|
23713
CCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGG
|
A
|
|
23714
GGCTCTCTGTACTGTCAGGTTTCAGCAAGGACTAACTCTTAATCTGCCC
|
C
|
|
23715
GGATCACCGGATTCGGGCCGTAAGGCCCCCATCATCGCGCCTCGCCCCG
|
A
|
|
23716
TGGTCTCCGCTCGTTCAGACAAGGTTTCACGTGTCTCGTCCTACTCTGG
|
A
|
|
23717
CAATCCCACTTTATGCCACCGGATCACTAAGTCCTACTTTCGTACCTGC
|
T
|
|
23718
GTCACCAAGTAGTATTTAGCCTTGGGGGGTGGGCCCCCCGTCTTCCCAC
|
C
|
|
23719
ATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAA
|
C
|
|
23720
TACCTCTCACGGTGACCATCCGACGCGGCACCTAAATGCCTTTCGGGGA
|
G
|
|
23721
CCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGG
|
G
|
|
23722
ATCACCAGTTTTACCCTAGGGCGCTCCTTGCGGTTACGCACTTCAGGTA
|
C
|
|
23723
GGAGGGCACCTTTAGAAGCCTCCGTTACGCTTTTGGAGGCGACCACCCC
|
A
|
|
23724
CTGGAGACCTTGGATATTCGGCCACAAGGATTCTCACCTTGTTCTCGCT
|
A
|
|
23725
GGGCTTTCACCCTCTTTGGCTGGCTTTCCCAAAACCATTCTGCTAGGAT
|
C
|
|
23726
GTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATT
|
G
|
|
23727
ATGCTACGCAGAGAAGTCCGGATATCAATGCCAGACTAGAGTAAAGCTC
|
C
|
|
23728
TCCGTATACTCTCAGGTTCGACTCTCCCCGCGGATTTGCCTACGGGAAT
|
C
|
|
23729
CTGGACCTATTCTCTGCGCCTCACATTGCTGTGAGGACCCTTTATCCCG
|
A
|
|
23730
TTAGCAGGTGGTCCGGATTCTTCTCCTCTCGGGCACGGACCTTAGCACC
|
C
|
|
23731
GCCTGTACACCTGCATCCTATCAACGTCATAGTCTTTGACGACCCTGAG
|
A
|
|
23732
AGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCG
|
C
|
|
23733
GGTTTGCCCTCCTGCCTCTTCGCTCGCCGCTACTGAGGCAATCGCTCTT
|
G
|
|
23734
ACCTTTCCCTCACGGTACTGGTACGCTATCGGTCAGACAGGTATGCTTA
|
G
|
|
23735
CCGGTCCTCTCGTACTAGGGACAGCTCCCATCAAATATCCTGCGCCCAC
|
G
|
|
23736
CCATTGGCATGACAACCCGAACACCAGTGATGCGTCCACTCCGGTCCTC
|
T
|
|
23737
ATGTGCTTGTAAGCACAGAGTTTCAGGTTCTTTTCACTCCCCTCCCGGG
|
G
|
|
23738
CCCTTCTCCCGAAGTTACGGGGTAATTTTGCCGAGTTCCTTAACAACCC
|
T
|
|
23739
CCTGAGTCGGTTTAGGGTACGGGCGCGTTATGCCCTCACGTCGAGGCTT
|
T
|
|
23740
ATCTGGGCTGTTTCCCTTTCGACAATGAAACTTATCTCACACTGTCTGA
|
C
|
|
23741
CGTATTTCAAGGATGGCTCCACAAACACTGGCGTGCCTGCTTCAAAGCC
|
T
|
|
23742
GGTCATTGCCTGCTTGCGGCTGACCATGGCTTATCGCAGCTGACCACGT
|
C
|
|
23743
CCTGGCGCGGGTAACCAGCATCTTCACTGGTACTTCAATTTCACCGGGT
|
G
|
|
23744
GTAACTCACAAGGCTGCACCTAAATGCATTTCGGGGAGTACGAGCTATC
|
T
|
|
23745
GTCGGTTTGGGGTACGGGCGGCCATAGCCCTCACGCCGAGGCTTTTCTC
|
G
|
|
23746
CACCGTCTATGGTCCCATTTTCCAAAGGGTTCTACTCATGAAATGTCTT
|
G
|
|
23747
CCGGCAACGCAACCCCCGACGGGTATCACGCGCAACCGGTTTGGTCTGA
|
T
|
|
23748
TTATCCTTCTGTGTCACTGCTTCATTCCATCGGTAGTGCAGGAATCTAC
|
A
|
|
23749
CAGAGCACCCCTTCTCCCGAAGTTACGGGGTCATTTTGCCGAGTTCCTT
|
A
|
|
23750
ATACTATCAGGTTCGATTCTCATGGTGGATTTGCCTGCCAAGATCAACA
|
T
|
|
23751
CTTACGGGGCTTTCACCCTCTCTGGCCGGCTTTCCCAAAACCGTTCTGC
|
T
|
|
23752
GACCGGCCTTCCCATGCCGTTCGGTTAACAACTTAAGTCCTAAATGCGG
|
T
|
|
23753
CGTTTATCCGATCCGTACGTAGTTGCCCAGCTATGCTCCTGGCGGAACA
|
A
|
|
23754
GTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTA
|
C
|
|
23755
GGTGCTTGTAAACACAAGGTTTCAGGTTCTTTTTCACTCCCCGTCAGGG
|
G
|
|
23756
GTAGGCGCACGGTTTCAGGAACTCTTTCACTCCCCTCCCGGGGTGCTTT
|
T
|
|
23757
ACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGC
|
C
|
|
23758
TTCCGTGTTCGGTATGGGAACGGGTGTGACCTCTTCGCTATCGCCACCA
|
A
|
|
23759
TCGCCTTAGGACCCGACTCACCCGGGGACGTTAACCGTGGCCCCGGAAC
|
C
|
|
23760
CACTCACCCACAACCATGGGCTCCCCATCATGCCTCAACCTTCACGCCC
|
A
|
|
23761
CTCCGAGACTTCATATGTGTCCCTGTGTTTAACTCTTTTGGTGGTGACG
|
G
|
|
23762
AAAATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTC
|
C
|
|
23763
GACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCAC
|
C
|
|
23764
CGAAGTTTGATAGGGTTCGGTAAGCTTTGTGGCCCCCTAGCCCATTCAG
|
T
|
|
23765
AGGCTTGCGCCGCCGCTTCGCCCCGATGGGGACGCTCTCCTACCCAGCG
|
T
|
|
23766
CGAACAGAGCGGTATTTCACCTTACGGCTCCGCGCGATCTGGCGACCGC
|
G
|
|
23767
ACCGTTCTACAAAAAGTACGCGGTTGTACTCGTATGGTACTTCCACAGT
|
T
|
|
23768
CGTTTCGCTCGCCGCTACTCAGGGAATCGCATTTGCTTTCTCTTCCTCC
|
G
|
|
23769
GCTACTTGGGACAACACGATCGGAAGACGGCTCACGTCCAGGTACGGGG
|
C
|
|
23770
AAGGTCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTT
|
T
|
|
23771
GTTCTGAACCCAGCTCGCGTACCACTTTAATCGGCGAACAGCCGAACCC
|
T
|
|
23772
TGATTCAAAGCCTCCGGCCTATCCTACACATCAATCACCCAAATTCAAT
|
G
|
|
23773
GTCTTTTCGTCCCATCGCGGGTAATCGGCATCTTCACCGATACTACAAT
|
T
|
|
23774
CCCCCCCCCCCCTTCCCCCCTCTCCTCCCCCTTCCCCCTTTCGCGCCCC
|
C
|
|
23775
CAGGTGTCACCCCATATACGTCATCTTTCGATTTAGCATAGAGCTGTGT
|
T
|
|
23776
CTCCACCAGACTAAAACGAGGCTAGCCCTAAAGCTATTTCGAGGAGAAC
|
C
|
|
23777
TTCCGTCAGCCGGCAGGACTGTCACTTCTCCGTCTCCACGTCACTCCAT
|
G
|
|
23778
CGCTAATTTTTCAACATTAGTCGGTTCGGTCCTCCAGTTAGTGTTACCC
|
A
|
|
23779
CTTGGCAGTGTGACATCACTAACTTCGCTACTAAACTTCGCTCCCCATC
|
A
|
|
23780
CCCGTTAAATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA
|
C
|
|
23781
CCCGGAGTACCTTTTATCCTTTGAGCGATGTCCCTTCCATGCGGAAACA
|
C
|
|
23782
TTCTCTGCGGCTCCATCGCTGCAGCACCCCTTCTCCCGAAGTTACGGGG
|
T
|
|
23783
AAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTG
|
T
|
|
23784
GCACAGCCATGTGTTTTTGTTAAACAGTTGCCTGGACCTATTCTCTGCG
|
C
|
|
23785
GCCAACATCCTGGTTGTCTGTGCAATTCCACATCCTTTTCCACTTAACT
|
A
|
|
23786
GGTCACCCGGTTTCGGGCCCATTATATGCAACTTAACGCCCTTTTCAAA
|
C
|
|
23787
TTATAGTTACGGCCGCCGTTCACTGGGGCTTCGATTCAATGCTTGCACA
|
T
|
|
23788
GTTTATCTGAGATTGGTAATCCGGGATGGACCCCTCAATCAAACAGTGC
|
T
|
|
23789
CGAAGTTACGGGGTCATTTTGCCGAGTTCCTTGACAATGCTTCTTCCGC
|
C
|
|
23790
GTCCACACACGCGTGTGTCCCTCATCAGTTCTCACCCTCCATGCCCCCC
|
G
|
|
23791
CCGGCCCGTCGGGGCCGGGACACACGCTCCCGCAACCCCGGCCACGCAA
|
C
|
|
23792
CCGGTACATTTTCGGCGCAGGGTCACTCGACTAGTGAGCTATTACGCAC
|
T
|
|
23793
CTCGAACTTCTTGTAAGCACACGGTTTCAGGTTCTCTTTCACTCCCCTT
|
C
|
|
23794
TTTCAGTTCAGGCGGTTCCCCCCGTATCCCTATGGATTCAGAATACGGT
|
G
|
|
23795
TCCGTTACATTTTGGGAGGCGACCGCCCCAGTCAAACTGCCTACCTGAC
|
A
|
|
23796
CCGCTCCTTCCATCAAGGTTCCACGTGTCTCGATGTACTCTGGATCCTG
|
C
|
|
23797
CCACGTGTTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCCCA
|
T
|
|
23798
GACTCCGTACTGTCAGGTTCGGCTCAACGGGTGGATTTGCCTGCCCATC
|
T
|
|
23799
ACGTGTCCGGCGGTACTCTGGATACAGATGGCTGTTCAGGCTTTTCGTG
|
T
|
|
23800
TGGGCTGTTTCCCTTTGGACAATGAAACTTATCTCCCACTGTCTGACTC
|
C
|
|
23801
ACATAGCTACCCAGCCATGCCCTTGGCAGAACAACTGGTACACCAGCGG
|
T
|
|
23802
CAGAGGTCAGTCCAACACGGTCCTCTCGTACTAGTGTCAGAGCCACGCA
|
A
|
|
23803
GTTTGATAGGGTTCAGTAACTTCTCAGCCCCTAGCCCATTCAGTGCTTT
|
A
|
|
23804
CGGCACCGGGCAGGCGTCACACCCTATACGTCCACTGTTCGTGTTGGCA
|
G
|
|
23805
AACCCAATAAATCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTG
|
C
|
|
23806
CCATACATCAATTATCTGGCATTCTGAGTTTGATAGGGTTCAGTAACCT
|
C
|
|
23807
CCTCCGTTACACTTTGGGAGGCGACCGCCCCAGTCAAACTGCCCGCCAA
|
G
|
|
23808
CTGTTATCCCCGAGGTAGCTTTTATCCGTTAAGCGACGGCTTTTCCACT
|
C
|
|
23809
TAGCCCATTCAGTGCTTTACCTCCGGTAATCTAAATCAACGCTAGCCCT
|
A
|
|
23810
TCCACAGCTCCTTACGGTACTGCTTCGTCCCGCATGCAATGCTCCTCTA
|
C
|
|
23811
CCATCGCGGGTAATCGGCATCTTCACCGATACTACAATTTCACCGAGCT
|
C
|
|
23812
CTGGACCTATTCTCTGCGCCCAACTCTCGTTGGGACCCTTTATCCCGAA
|
G
|
|
23813
CTTTTACCTTTACACTCTACGATTGATTTCCAACCAATCTGAGCCAACC
|
T
|
|
23814
TTATAGTTACGGCCGCCGTTTACCGGGGCTTCAATTCAAAGCTTCATAT
|
T
|
|
23815
GCCATTAAGATTCTCACTTAATTCTCGCTACTTATTCCGGCATTCTCAC
|
T
|
|
23816
GGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCG
|
T
|
|
23817
CTTCTCCCGCTGGCCTTAGAATCTTCTTCCTATCTACCTGTGTCGGTTT
|
G
|
|
23818
TTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCA
|
C
|
|
23819
GCTAGTCCTAAAACTATTTCGGGGAGAACCAGCTATCTCCGGGTTCGAT
|
T
|
|
23820
CCTCCGGCCGGTTTCACGGCCGCAAGTTAGAATTCCAGCACTACAAGAG
|
T
|
|
23821
TGTTCGTCCCGTCCTTCATCGGCTCCTAGTGCCAAGGCATCCACCGTGC
|
G
|
|
23822
GCCAGGCCTTCAAGCCTGTTCCCCTGGCTAGCCGCTTTATGACTCCCGC
|
C
|
|
23823
CTTTCTTTTCCTCCGGCTACTTAGATGTTTCAGTTCACCGGGTTCCCTT
|
C
|
|
23824
ATGATTCTCACATAATTCTCGCTACTCATTCCGGCATTCTCACTCGTAT
|
G
|
|
23825
CGGGCACGGACCTTAGCACCCATGCCCTTACTGCCGGACTGCAGACCGT
|
G
|
|
23826
GTGAGTTTCCTCATTCAGAGATCTCCGGATCAATGCTTATTTGCAGCTC
|
C
|
|
23827
TAAATGCAGTCCGAACCCCGGAGTGCACGCACTCCGGTTTGGGCTCTTT
|
C
|
|
23828
GCCCAAGGGTAGATCACTTGGTTTCGCGTCTACTCCTTCCGACTATACG
|
C
|
|
23829
AGCTTAGCGGATTTTCTCGGGAGTCTGATTACCGGCGCTATTGGATTCC
|
A
|
|
23830
CTCGCAGTCAAGCTCCCTTCTGCCTTTGCACTCTCCGAATGATTTCCAA
|
C
|
|
23831
GTCTAGTCCCACGTACTTGTGCGCCCTGTTCAGACTCGCTTTCGCTCCG
|
C
|
|
23832
TTCTCCGCTATCCACACCTCATCGCCACCCTTTTCAACGGATGTGCGTT
|
C
|
|
23833
GCCGGCTCCCATTCCGTGTCACCCCTGCGCTCACCTACCACGGCTACGC
|
T
|
|
23834
TCCCGGGGTCCTTTTCACCTTTCCTTCACAGTACTATGCGCTATCGGTC
|
A
|
|
23835
CCAACATCCTGGTTGTCTGTGCAATTCCACATCCTTTTCCACTTAAATC
|
C
|
|
23836
GCTGGCGCCGCGGCTTCGAAGCCTCCCGCCTATGCTACACAATCCGCAC
|
C
|
|
23837
ACGCCCAATAATTCCGGACAACGCTTGCCACCTACGTATTACCGCGGCT
|
G
|
|
23838
CCCTACCAGGTATCACATGCACACGGTTTAGCCTCATCCACGTTCGTTC
|
G
|
|
23839
AGCACCGGGCAGGTGTCAGGCTGTATACGTGATCTTTCAATTTGGCACA
|
G
|
|
23840
CTCCCCATCATGCCTCAACCTTCACGCCCAGCGGATTTACCTACCAGAC
|
A
|
|
23841
CTTCAACTTAACCTCGCACGTAAACGTAACTCGCCGGTTCATTCTACAA
|
A
|
|
23842
AGAGTAGCCATAACACAAGGGTAGTATCCCAACAACGCCTCAGTCGAAA
|
C
|
|
23843
GCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACT
|
T
|
|
23844
CATAGACCTGTGTTTTTGCTAAACAGTTGCTTGAGCCTATTCTCTGCGG
|
C
|
|
23845
ACACACAACCCCTACCAAGTATCACATGCACACGGTTTAGCCTCATCCA
|
C
|
|
23846
TCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGG
|
C
|
|
23847
CATTCGGATATCTCTGGATCAAGGCTTACTTACAGCTCCCCAAAGCATG
|
T
|
|
23848
GCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTT
|
T
|
|
23849
TCTTTTCGTCCCATCGCGGGTAATCGGCATCTTCACCGATACTACAATT
|
T
|
|
23850
TGTACCCCCCATTGTAACACGTGTGTAGCCCCGGACGTAAGGGCCGTGC
|
T
|
|
23851
TCCCCGGAGTACCTTTTATCCTTTGAGCGATGTCCCTTCCATACGGAAA
|
C
|
|
23852
CGTTGAGCGATGGCCCTTCCTTTCGGTACCACCGGATCACTAAGCCCGA
|
C
|
|
23853
TTCAAGGGGTCTTACTCGTTATACGATGGGATATCTAATCTTGGAGTCG
|
G
|
|
23854
CCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTAC
|
T
|
|
23855
ACCTTGGTCTTACGGCGGGAGGGAATCTCACCCTCCTTATCGTTACTTA
|
T
|
|
23856
CGTGCCCCGCCCTACTCAGGATACTGCTAGCCACGATCAACTTTTAGGT
|
A
|
|
23857
CACCCTCAGTTCATCCGGAAGCTTTTCAACGCTTATCGGTTCGGTCCTC
|
C
|
|
23858
TCTACCTCCATGAGACTAATACGAGGCTAGCCCTAAAGCTATTTCGAGG
|
A
|
|
23859
TACCTGTGTCGGTTTGCGGTACGGGCACCTTAGCATACACTAGAACTTT
|
T
|
|
23860
AGCGGTTCCACAGCTTGTAAACATATGGTTTCAGGTTCTCTTTCACTCC
|
C
|
|
23861
TTATAGTTACGGCCGCCGTTCACTGGGGCTTCGGGTCAAAGCTTGCACT
|
C
|
|
23862
TTATAGTTACGGCCGCCGTTTACTGGGGCTTCGGTTCGATGCTTCGATT
|
G
|
|
23863
GCCTTACGGGGTGGTCCCCGCTCATTCCCACAAGGTTTCTCGTGTCTCG
|
T
|
|
23864
CCGGAGTTTTTCACACTGAGCCATGCAGCTCTGTGCGCTTATGCGGTAT
|
T
|
|
23865
CTTCTCCCGTTGGCCTTAGAATCTTCTTCCTACCTACCTGTGTCGGTTT
|
G
|
|
23866
TGCCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTACAGCCC
|
C
|
|
23867
GGAGTTCTTCGTGATATCTAAGCATTTCACCGCTACACCACGAATTCCG
|
C
|
|
23868
AGTGATGGGCAGGTTGGATACGCGTTACTCACCCGTGCGCCGGTCGACG
|
C
|
|
23869
TCACGGTACTCGTACGCTATCGGTCAGACAGGTATACTCAGGCTTACCC
|
G
|
|
23870
ACGCATTCGGAGTTTGTCAAGACTTGATAGGCGGTGAAGCCCTCGCATC
|
T
|
|
23871
CATCATCTGTATGGCATTCGGAGTTTGATATCCCTTAGTAAGCTTTGAC
|
G
|
|
23872
TTCTCCGCTATCCACACCTCATCGCCACCCTTTTCAACGGATGTGCGTT
|
C
|
|
23873
AAGCACTTTGGTTTGGGCTGTTCCCCGTTCGCTCGCCGCTACTTAGGGA
|
A
|
|
23874
CACTTATGCCCGATTATTATCCACGCCAAACTCCTCGACTAGTGAGCTG
|
T
|
|
23875
CTTAGGACCCGACTCACCCAGGGCAGACAAACTTGACCCTGGAACCCTT
|
G
|
|
23876
CTCATCAGTTCTCACCCCCAATGTCCCCCGGATTTACCTGAGGGACGGG
|
C
|
|
23877
CCCATGGTGCACGCACCATGGTTTGGGCTCTTCCGCGTTCGCTCGCCGC
|
T
|
|
23878
GCTAGTCCTAAAACTATTTCGGGGAGAACCAGCTATCTCCGGGTTCGAT
|
T
|
|
23879
ACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACG
|
T
|
|
23880
CATTCCGGCATTCTCACTCGAATACAATCCACCGCTGCTTCCGCTACGA
|
C
|
|
23881
GTTTCAGTTCGCCGGGTACCTCTCTTGCAGGCCATGTATTCACCTGCAG
|
A
|
|
23882
ACCTGAGGCTACTCGCCTCGACTACCTGTGTCGGTTTGCGGTACGGGTA
|
G
|
|
23883
AAGGCTAGCCCTAAAGCTATTTCGAGGAGAACCAGCTATCTCCGGGTTC
|
G
|
|
23884
ATTATTATTTTCTCCTCCTACGGGTACTGAGATGTTTCACTTCCCCGCG
|
T
|
|
23885
GCTTGCGCTAACCTCTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAG
|
C
|
|
23886
CAGAGGTCTGTCCAACACGGTCCTCTCGTACTAGTGTCAGAGCCACGCA
|
A
|
|
23887
ATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTACCCCA
|
C
|
|
23888
TCACGCAGAATTCCTCGTGCTCCGCGCTACTCAGGATACCACTAGGCTT
|
C
|
|
23889
CGCGTCTTCGGTGGCGTGCTTGAGCCCCGCTACATTGTCGGCGCGGAAC
|
C
|
|
23890
TACTTATGCCCGATTATTATCCACGCCAAACTCCTCGACTAGTGAGCTG
|
T
|
|
23891
ACCGTAGTGCCTCGTCATCACGCCTCAGCCTTGATTTTCCGGATTTGCC
|
T
|
|
23892
AGCTGACGCCTGTATTTCCCAGTCTCCCACCTATCCTGTACATGAAATA
|
C
|
|
23893
GGCGTTGCTGATCCGCGATTACTAGCGACTCCGCCTTCACGGAGCCGGG
|
T
|
|
23894
GGGTGCCGCATGGGTTAAGCTTAGCGGATTTTCTCGGGAGTATGGTTAC
|
C
|
|
23895
TCTTCAGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACTTCCT
|
C
|
|
23896
CGCCGGCACCGGATCACTATCTCCGACTTTCGTCCCTGCTCGATCCGTC
|
G
|
|
23897
CACACTATCCGTCTCCGTCACTCCTTCGCTCCATATACGGGTGCAGGAA
|
T
|
|
23898
ACTGTCAGGTTCGACTCTTCCTGCGGATTTGCCTGCAGGAATCAACATC
|
T
|
|
23899
TCTTTCGGCGAGGGGGTTTCCCACCCCCTTTATCGTTACTTATACCTAC
|
A
|
|
23900
CTTTTCAGTGCTCTACAGGACACATCCATCACCTGAGGCTGTACCTCAA
|
T
|
|
23901
ATGACCCTCCCCGGTTGAGCCGGGGGCTTTCACATCAGACTTAAGAAAC
|
C
|
|
23902
TTTCACAACTGACTTAAATATCCATCTACGCTCCCTTTAAACCCAATAA
|
A
|
|
23903
CTACTTATTTTCGGTCCCTTACGCCCGGGTCAACCAACGCCCGGGTCCA
|
G
|
|
23904
GTATTTAGGCTTACCGGGTGGTCCCGGCAGATTCACAGCAGATTCCACG
|
A
|
|
23905
CTTCAACCTGGACATGGATAGGTCACCCGGTTTCGGGTCTGCACACACT
|
G
|
|
23906
TCCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCGTTTAC
|
G
|
|
23907
GGTCACCCGGTTTCGGGCCCATTGTATGCAACTTAACGCCCTTTTCAAA
|
C
|
|
23908
GGCTACACATTTTAAAATGCTTAACCTTGCCGGAAAAAGTAACTCGTAG
|
G
|
|
23909
CAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGT
|
T
|
|
23910
GCCAGGGTAGTATCCCACCGATGCCTCCACCGAAGCTGGCGCTCCGGTT
|
T
|
|
23911
TTCACTGAAGGGTAACACCCCATAACAGGTGCCAGGTTTCCCCATTCGG
|
A
|
|
23912
TCCAGCTAATCAGACGCGGGTCCATCTTATACCACCGGAGTTTTTCACA
|
C
|
|
23913
CTTTATGAATATGCTTAGCGGATTTTCTTGGGAGCCTGATTACGTCCAT
|
T
|
|
23914
CATCAGGTAGTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGA
|
A
|
|
23915
CATGCACCACGGATTTGCCTATGATGCGCGCTGCGTGCTTGACCACGGA
|
A
|
|
23916
GACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTACCCGA
|
C
|
|
23917
TCACTGCTTTAAGCAGCTCCGACCGCTTGTAGGCGCACGGTTTCAGGAA
|
C
|
|
23918
GCTCCCAACACCACGCGGCGATACCAACCCGAAGGAAGGAACCACCACG
|
A
|
|
23919
GACTTCCCATTCCATTCCACTAAACCTTTACAATACCGTTTTCTGTCCG
|
A
|
|
23920
ACTTAACGACCCGTCTGCGCTCCCTTTAAACCCAATAAATCCGGATAAC
|
G
|
|
23921
GGGGTGGGTTTCATACTTAGATGCTTTCAGCAGTTATCCGCTCCGCACT
|
T
|
|
23922
GAAATCCTCGGATCAAAGCCCTGCTGGCGGCTCCCCGAGGCATATCGCA
|
G
|
|
23923
CTTTCATGGCCCCTACTGATCATCGCCTTGGTAGGCCATTACCCTACCA
|
A
|
|
23924
CTGTTATCCCCAGGGTAACTTTTATCCGTTGAGCGATGGCATTTCCACT
|
C
|
|
23925
CCTACCCTCAGCTCATCCAGAAGCTTTTCAACGCTTATTGGTGCGGTCC
|
T
|
|
23926
ACCAAGAAGGTGCTCCGACCGCTTGTAGGCACATGGTTTCAGGAACTAT
|
T
|
|
23927
CTTCTCCCGTTGGCCTTAGAATCTTCTTCCTACCTACCTGTGTCGGTTT
|
G
|
|
23928
CCTGGCCAAGGGTAGATCACTTGGTTTCGCGTCTGCCACTGCCGACTAT
|
A
|
|
23929
GGGGGTCTCCCTTATGCCGAAGGCACGGGAGCAATTTGCCGAGTTCCTT
|
G
|
|
23930
CATGGTTTAGCCCCGTTACATCTTCCGCGCAGGCCGACTCGACCAGTGA
|
G
|
|
23931
ATCCGCCGCCTTTTCAACGGAGGTCGGTTCGGTCCTCCATGGAATTTTA
|
C
|
|
23932
CCAAAGTCAATGCTAAGCTGTAGTAAAGGTTCACGGGGTCTTTTCGTCC
|
C
|
|
23933
AAAGTTCGGTGGTTACGGAATTTCTACCGTATGTGCATCGACTACGCCG
|
T
|
|
23934
CAGGTGTCAGCCCCTATACTTCATCTTTCGATTTAGCAGAGACCTGTGT
|
T
|
|
23935
ACTTAAAGCCAGCGCCCCTTCTCCCGAAGTTACGGGGCCATTTTGCCGA
|
G
|
|
23936
ACTTAGATGCTTTCAGCACTTATCCGATCCAGACTTAGATACCCGGCAA
|
T
|
|
23937
CTACAGGATTTAGTTTAGCGGATTTTCTTGGCAGCATGATTACATGCAC
|
T
|
|
23938
CCTTAACCTTCCGGCACTGGGCAGGTGTCAGCCCGTATACGTCGTATCT
|
C
|
|
23939
TGAGCCAACATCCTAGTTGTCTTCGAAATCCCACATCCTTTTCCACTTA
|
A
|
|
23940
CAGGATGTGACGAGCCGACATCGAGGTGCCAAACCCCTCCGTCGATATG
|
A
|
|
23941
GGTTTTGCCGGTCCATGGTCGGTACGGGAATATCCACCCGTTCATCCAT
|
T
|
|
23942
CTTTACGCTATCGGTCATTGGGTAGTATTTAGGCTTGGAGGGTGGTCCC
|
C
|
|
23943
GCATGGATTAAGTTTAGCGGATTTTCTAGGAAGTATGATTACCTACGCT
|
A
|
|
23944
ACTGTCCATCCTCTGGTTTCACAGAGCTATGTTAGAATTTCAGTAACCG
|
A
|
|
23945
ACCTCGCGGTACGCCTTCGACGCCGACTGGAATGCTCCCCTACCGATCA
|
T
|
|
23946
CTCTTGCGATGAGCTCTCCTCTTAACCTTCCAGCACCGGGCAGGTGTCA
|
G
|
|
23947
AGCTGACGCCTTGGCTTCCCAGTCTCCCACCTATCCTGTACATGTAATA
|
C
|
|
23948
GAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTGTCACTGGGACCAG
|
A
|
|
23949
TGAGCCAACATCCTGGTTGTCTACGTATCTTCACATCGTTTTCCACTTA
|
A
|
|
23950
TGAGGGCACCTTTAGAAGCCTCCGTTACGCTTTTGGAGGCGACCACCCC
|
A
|
|
23951
TTAAATCGACCGAAGTTTCAATAAAGTAATTCCCGTTCGACTTGCATGT
|
G
|
|
23952
AGTCGGGTTGCAGACTCCAATCCGAACTGAGAGAGGCTTTAGGGATTAG
|
C
|
|
23953
CCTGTGTCGGTTTACGGTACGGGTATGGTATGAACAATAGCGGCTTTTC
|
T
|
|
23954
CTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCA
|
C
|
|
23955
AAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTC
|
C
|
|
23956
CCTGAGTATATTCAACCCGACTACGTGTGTCCGTTTACGGTACGGGTAC
|
C
|
|
23957
ACCACGAATTCCGCCTGCCTCAACTGCACTCAAGATATCCAGTATCAAC
|
T
|
|
23958
AGTGAGCTATTACGCACTCTTTTAATGAGTGGCTGCTTCTAAGCCAACA
|
T
|
|
23959
GGCTCACGCCCCGCCTTCAACGCCGAGTGGAATGCTCCCCTACCGATGA
|
T
|
|
23960
AGGGCACCTTTAGAAGCCTCCGTTACACTTTTGGAGGCGACCACCCCAG
|
T
|
|
23961
CTCTGCCATCGCCATCGCCGTTCGGCTTAGACTTAGGACCCGACTGACC
|
C
|
|
23962
GCCGAGTTCCTTAACAAGGGTTCTCCCGCTCGTCTTAGGATTCTCTCCT
|
C
|
|
23963
CTCCCCCCCCCCCCTTCCCCTCCGCGGCCACCTTTCCCCCCCCCTCCCC
|
A
|
|
23964
CCCATATACACGGGTTAGAATCCAAACAAATGAAGGGTCGTATTTCAAC
|
A
|
|
23965
CCCGCATCAGCGGGTTAGAACTCAAATAATCAAAGGGCCGTATTTCAAC
|
A
|
|
23966
CTTCACAGTACTATACGCTATCGGTCACTGGGTAGTATTTAGGGTTGGA
|
G
|
|
23967
CATTCCCACTTAATACCACCGGATCACTAAGCCCTACTTTCGTACCTGC
|
T
|
|
23968
CTTCCGTCGCCCCGCGGTGGTTTCACTGCTCCGTCTCCACGTCGCCCCA
|
T
|
|
23969
GCGGGTAACCTGCATCTTCACAGGTACTAAAATTTCACCGAGTCTCTCG
|
T
|
|
23970
AAAAGTACGCGGTTGAGCTAATAATGCTCTTCCACAGCTTGTAAACACA
|
G
|
|
23971
CGGTACGGGAATATCAACCCGTTCATCCATTCGACTACGCCTGTCGGCC
|
T
|
|
23972
CCTCATCTACCTGTGTCGGTTTGCGGTACGGGCGCCTTAGTATACCTCA
|
T
|
|
23973
GTAGTATTTAGCCTTGGAGGGTGGTCCCTCCTGCTTCCCACAGGGTTTC
|
A
|
|
23974
TTCCGTCAGGTGGCGGCACTTACGTTCCTTCGTCTCTCCATCGAGGTAT
|
A
|
|
23975
CTTCAAAGTCTCCGGCCTATCCTACACATCAATTACCCAAATTCAATGT
|
T
|
|
23976
CTCTCAGGGCTCTTACTAACTGAACGTTATGGGAAATCTCATCTTGAGG
|
G
|
|
23977
AAGTCCTCGAGCGATTAGTATTGGTCCGCTTCACGTCTCACAACGCTTC
|
C
|
|
23978
ACGCCTTTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT
|
T
|
|
23979
CCTGATCGACTTGTATGTCTCCCAGTCAAGCGCCCTTATGCCATTACAC
|
T
|
|
23980
CGTTTTCCACTTAGCATGTATTAGGGACCTTAGCTGTGGGTCTGGGCTG
|
T
|
|
23981
TAGTCAAGTATCGTCTCTCTTCTTCCTTGCTGATAGACCTTTACATACC
|
G
|
|
23982
GACACATGGTTTTCTGCAACTGCCGGCCGGCCCGTCGGAGCCGGCGCAC
|
G
|
|
23983
TTTCTCGTGTCTCGTGGTACTCTGGATCCCGCCTTGCCGCTCCCGGTTT
|
C
|
|
23984
CTAATGAGATGTTTCAGTTCACAGCGTTTACCTCCAACTAGACTATGAA
|
T
|
|
23985
ATCCTTTCCCACTTAGCACGCGCTTGGGGACCTTAGACGACGATCTGGG
|
C
|
|
23986
GTTTCACGTGTCTGGCCGTACTCTGGAACTCGCTCAGCTCTTGTCGTTT
|
T
|
|
23987
ATGGTTATAGTTACCACCGCCGTTTACCGGGGCTTGAATTCACCGCTTC
|
G
|
|
23988
CCGCACGGAATGGCCGTCTCGTCTCGGGGGGGGCTTCCCGCTTAGATGC
|
T
|
|
23989
TGCTCGACTTGTCTGTCTCGCAGTCAAGCTCCCTTATACCTTTACACTC
|
T
|
|
23990
ATGCATTGCCAGAAGCTTTTCCTGGAAGCCGTCATCATGTGCTTCGCTA
|
C
|
|
23991
TCTTGCGGCGAGCAGGTTTCTCACCTGCTTTATCGTTACTTATACCTAC
|
A
|
|
23992
CGCGCACGCAACCCCCGACGGGTATCACGCGCACGCGGTTTGGTCTGAT
|
C
|
|
23993
CGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCA
|
T
|
|
23994
GACAGTGCCCAAATCATTACGCCTTTCGTGCGGGTCGGAACTTACCCGA
|
C
|
|
23995
TCCCATCTATCCTGTGCATGCAACACCGAAACCCAATATTAGGCTACAG
|
T
|
|
23996
CCCGGGTCATGCCCTTTCAGAGTGTCCCTCTGCTTAAAACTTTCGGTGG
|
T
|
|
23997
GGGATCCCATTCCCGGCTTCCGCTCTCTGCACGTGTCCCCACAGTTCTG
|
T
|
|
23998
CACCTCGCCATACACGCCGCACGGATTTGCCTATGCGACTGGCTGCGTG
|
C
|
|
23999
TCGCTCCTCAGCGTCAGTTACAGACCAGAGAGTCGCCTTCGCCACTGGT
|
G
|
|
24000
TATCGAACCATAACGGCTCCCATCATCACACCTCGCCATGCATGCCATG
|
C
|
|
24001
TTCACCGGGGCTTCAATTCGGAGCTTGCACCCCTCCTCTTGACCTTCCG
|
G
|
|
24002
CTGCAGGATTAAGTTTAGCGGATTTTCTCGGCAGCATGCTTACGCGCAC
|
T
|
|
24003
TCTCCTACCATACCTATAAAGGTATCCACAGCTTCGGTAATATGTTTTA
|
G
|
|
24004
GGGCGCGTCATGCCCTCACGTCGAGGCTTTTCTCGGCAGCATAGGATCA
|
C
|
|
24005
CTCCGACGGATTGTAGGCGCACGGTTTCAGGAACTCTTTCACTCCCCTC
|
C
|
|
24006
CACTCGACTAGTGAGCTATTACGCACTCTTTGAATGAATAGCTGCTTCT
|
A
|
|
24007
ACTCCCCTCGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCA
|
C
|
|
24008
CCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTATGCGCTATCG
|
G
|
|
24009
CTGGTCCTCTCGTACTAGGAGCAGATCCTCTCAAATTTCCTTCGCCCGC
|
G
|
|
24010
ACTTTCGTTACTGCTCGGGCCGTCACCCTCGCAGTTAGGCTAGCTTTTG
|
C
|
|
24011
TGTAATAGCCACGTAATTTAAAACTGAAATTGAGAGAGACTTACCCAGA
|
G
|
|
24012
GGTGGTCTACCGGGAGACTTACCCTCATGTGAGGTGGGAATACTCATCT
|
T
|
|
24013
TGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG
|
T
|
|
24014
TCTCCACATCACTCTTATAGGTAGTACAGGAATATTAACCTGTTCTGCC
|
A
|
|
24015
CCATTCTGAGGGTACCTTTGGGCGCCTCCGTTACTCTTTCGGAGGCGAC
|
C
|
|
24016
GATGGCAGGACTGTCACTTCTCCGTCTCCACATCGCTCCATAAAGTAGT
|
A
|
|
24017
TCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGG
|
T
|
|
24018
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAGTATTCCCCACT
|
G
|
|
24019
CGGACATCCTTAATGACATTCGCAGTTTGATTGTATTCAGTACCCCGGG
|
A
|
|
24020
TACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCT
|
T
|
|
24021
TTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG
|
A
|
|
24022
CGGAGGCGACCGCCCCAGTCAAACTCCCCGCCTGGCATTGTCCCACCGC
|
C
|
|
24023
ACCTTTTAGGAGGCGACCGCCCCAGTCAAACTGCCCGTCAGACACTGTC
|
T
|
|
24024
ACAGCCCAGCCTTCCGTTGTGCGTACTTCACTACACAACAGCCTCACTG
|
C
|
|
24025
TCATACCACCGGAGTTTTTACCCCTGCACCATGCGGTGCTGTGGTCTTA
|
T
|
|
24026
CACTCACCCGAAGGCTTGCTCCCAAACAAAAGAGGTTTACAACCCGAAG
|
G
|
|
24027
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTC
|
G
|
|
24028
ACTTTCGTTCCTGCTCGACTTGTCAGTCTCGCAGTCAGGCTGGCTTGTG
|
C
|
|
24029
CCACCAGGGAGGCTCCGACGGTTTGTGGGCGCACGGTTTCAGGAACTGT
|
T
|
|
24030
ACTGGCGTGCACGTCTCTTTGTCTCCCACCTATCCTGTACATGTATGAC
|
C
|
|
24031
TGATAGCGTGAGGTCCGAAGATCCCCCACTTTCTCCCTCAGGACGTATG
|
C
|
|
24032
AAATCTTTAATCTCTTTCAGATGTCTTCTAGAGACGTCATTGGGTATTA
|
G
|
|
24033
CACCGGGGCCCCAAGACCCACACACACCAACAAACCCGAAGGCTTAGTG
|
G
|
|
24034
TACTTTTCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTCCAATAA
|
A
|
|
24035
CTCTGCCTATCCTTCTGTGTCACTGCATCCGGTTGCTCGGCGGTATCGG
|
A
|
|
24036
ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATC
|
G
|
|
24037
AACATCCTGGTTGTCTAAGCAACTCCACATCCTTTTCCACTTAACGTAT
|
A
|
|
24038
CTCCGGCCGGGCCCGCCAGGACCCGGACACACGCTCCCTCAACACCACG
|
C
|
|
24039
TTCTCTGCGGCTCTTTCGAGCACTCCTTATTCCGAAGTTACGGAGTCAA
|
T
|
|
24040
GGCACAGCCCTGTGTTTTTGTTAAACAGTTGCCTGGACCGATTCTCTGC
|
G
|
|
24041
TGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCG
|
C
|
|
24042
ATGCGTCCCACGGATTTGCCTATGGGACGGGCTGCGTGCTTGACCACGG
|
A
|
|
24043
CCCAGACAACCATCGCTGGGGTTGAGCTACCTCCCTGCGTCCCTCCGCA
|
G
|
|
24044
ACGCCGTTAGGCCTCACCTTAGCTCCCGACTGACCTGGAGCGGACGAAC
|
C
|
|
24045
GCCTTTAGCCTTAACCTTGCCAGCCGGCGTAACTCGCCGGACCGTTCTA
|
C
|
|
24046
TGGCCGTTCAACCTCTCAGTCCGGCTACTGATCGTCGCCATGGTGAGCC
|
G
|
|
24047
CGCTTTCGCTCGCCACTACTCACGGAGTATCCCTTCCTGCAGGTACTGA
|
G
|
|
24048
AGGACCCGACTCACCCGGGGACGACGAACGTGGCCCCGGAACCCTTGGT
|
C
|
|
24049
CATTGCGGAAGATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTG
|
T
|
|
24050
GCATGTATTAGGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACT
|
C
|
|
24051
CCCGTTACCCATCATCGCCATGGTAGGCCTTTACCCTACCATCTAGCTA
|
A
|
|
24052
GCCCTCACCCGATTAGTAACAGTCAGCTCCATGTGTTGCCACACTTCCA
|
C
|
|
24053
ACCCCAAGTCATCCCCCGGTTTTCAACCCAGGTGGGTTCGGTCCTCCAC
|
G
|
|
24054
CGCCTTAGGACCCGACTAACCCAGGGCGGATAAACCTAGCCCTGGAACC
|
C
|
|
24055
TTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCA
|
C
|
|
24056
GTACGGGTAACACAGAAATATGCTTAGCGGGTTTTCTTGGGAGCCGGTT
|
T
|
|
24057
AAGCTCCATGGGGTCTTTCCGTCTTGTCGCGGGTAACCGGCATCTTCAC
|
C
|
|
24058
AACTTTATTCCCTTATAGAAGCAGTTTACAACCCATAGGGCCGTCTTCG
|
T
|
|
24059
GGGCGGGATTCGCACCCGCCTCTCGCTACTCATGTCTGCATTCTCACTC
|
C
|
|
24060
ATACTATCAGGTTCGGATCTCATGGTGGATTTGCCTGCCATGATCGACT
|
C
|
|
24061
ACGCCGTCGGGCATATAAAGCCCTCCGACAGTTTGTAAACACAGGGTTT
|
C
|
|
24062
GCCTATCGACCACGTGTTCTGCATGGGGTCTTCAGCGGCTCGGGGCCGC
|
A
|
|
24063
GGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACAC
|
G
|
|
24064
GCCCCCGAGCCTTGGCAGTGCTCTACACGGCGTGAGGTTCATCCGAGGC
|
T
|
|
24065
TTCCTTAACCAAGAATCTCTCAACGCCTTAGTATGTTCTACCCGACCAC
|
G
|
|
24066
TTTCCCTGCGGCTCCGGGACTTTATCCCTTAACCTTGCCAGTATGCACA
|
A
|
|
24067
TACTGTCAGGTTCGACTCTTGCACCGGATTTGCCTGGCACAATCAACAT
|
C
|
|
24068
GCCTTCCCATGCCATTCTGCTAGATACCTTCCATACCGTGCGCTGTCCG
|
A
|
|
24069
ATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGG
|
G
|
|
24070
TTCGGCTCAAAGTCCGGATTTGCCTGGACCTCTCATCACCTACACTCTT
|
C
|
|
24071
ACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTCA
|
A
|
|
24072
TTTCCGTTTCGCCTACGGGGCTCTCACCCTCTCTGGCCGGTCTTTCCAG
|
A
|
|
24073
GCCCCGGACAACCATCGCCGGGGATGAGCTACCTCCCTGCGTCCCTCCG
|
C
|
|
24074
TGTCGCGGGTAACCGGCATCTTCACCGGTACTACAATTTCGCCGGGCGG
|
G
|
|
24075
AAGCCCTCGATCTATTAGTACACACTTGCTGAATGGATCGCTCCACTTA
|
C
|
|
24076
CCTTGGCAACAGTTCTCTCGCTCACCTCGGGATACTCTCCCTGCCCACC
|
T
|
|
24077
TCTCCGCCAAAGCCAAAGCCTTGGTTTCCCAGAGTCCCATCTATCCTGT
|
G
|
|
24078
AGGAGTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGAGATTT
|
C
|
|
24079
CAGGATGTGACGAGCCGACATCGAGGTGCCAAACCACTCCGTCGATATG
|
A
|
|
24080
CAACCTGTTGTCCATCGGCTACGCTTTTCAGCCTCACCTTAGGTCCCGA
|
C
|
|
24081
TCAGATGGCGGCACTGCCACGACTCCGTCTCCACGTCACTCCCCAAGGT
|
A
|
|
24082
CTACGGGGCCATCACCCTCTGCGGCCCGGCATTCAATCCGGTTCGCCTC
|
A
|
|
24083
CCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCG
|
G
|
|
24084
CCTTTAATCATGTGAACATGCGGACTCATGATGCCATCTTGTATTAATC
|
T
|
|
24085
TTTTCACACCTGACTTAAGATCCCGCCTTAAGCTTCCCTTTACACCCAG
|
T
|
|
24086
CCTACCCTCAGCTCATCCAGAAGCTTTTCAACGCTTATTGGTGCGGTCC
|
T
|
|
24087
GTCACACTGAGTATTTAGGCTTACCGGGTGGTCCCGGCAGATTCACAGC
|
A
|
|
24088
CCAGGATAACTTACGTACACCATTCGACGCCGTGAGTATGCTCCCCTAC
|
C
|
|
24089
AGAGAACCAGCTATCTCCAAGTTCGTTTGGAATTTCTCCGCTACCCACA
|
A
|
|
24090
CCCGAAGTTACGGGGTAATTTTGCCGAGTTCCTTAACAACCCTTCTCCC
|
G
|
|
24091
GGCTCACGCCCCACCTTCGACGCGGAGTGGAATGCTCCCCTACCGATGT
|
T
|
|
24092
GTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTGAGCGTCAGTCTT
|
C
|
|
24093
CGCGAGTCCATCCTGAAGCGAATAAATCCTTTTCCCTCAGCACCATGCG
|
G
|
|
24094
TTATCGCAGCTTATCACGTCTTTCTTCGGCTCTTAGTGCCAAGGCATCC
|
A
|
|
24095
CGGCAAAGATTCTCACTTTGCTCTCGCTACTCATGCCGGCATTCTCTCT
|
C
|
|
24096
CCGGCAGACCGATCAAGAAAAAACCCACAACCCCGCACGCGCAACCCCT
|
G
|
|
24097
GGGCTGTTTCCCTTTTGACTATGAGACTTATCTCACATAGTCTGACTGC
|
T
|
|
24098
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG
|
T
|
|
24099
TTGTGACTATTCTCTGCGGCCTGCTCTCGCAGGCACCCCTTATCCCGAA
|
G
|
|
24100
TTACCTCCACTTCAACCTGGACATGGGTAGGTCACCCGGTTTCGGGTCG
|
A
|
|
24101
TCGCAAGGTTATCCCCAAGTGAAGGGCAGGTTGGATACGCGTTACTCAC
|
C
|
|
24102
CGCGATCGGCAGACCATGCGCGTTCAGGTACGGGGCCCTCACCCTCTGC
|
G
|
|
24103
GCCTTTCACTCCTACACTCGGCTCATCCAGAAGCTTTTCAACGCTTATT
|
G
|
|
24104
AGTTTGATAAGGTTCAGTAACCTCTCGGCCCCTAGCCAATTCAGTGCTT
|
T
|
|
24105
GGCTGCAACACGGTGACGTGAAGCGAATCCCAAAAACCATCTCTCAGTT
|
C
|
|
24106
CCGGTCTCTCGACTAGTGAGCTGTTACGCACTCTTTGAATGAATGGCTG
|
C
|
|
24107
GGATCACTAACTCCAACTTTCGTTACTGCTCGAACTGTCGCTCTCGCAG
|
T
|
|
24108
CTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTA
|
C
|
|
24109
CGGCTACGCCTTTCGGCCTCACCTTAGCTCCCGACTAACTTGGAGCGGA
|
C
|
|
24110
ACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTA
|
G
|
|
24111
ATACTGTCAGGTTCGACTCTTGCACCGGATTTGCCTGGCGCAATCAGCA
|
T
|
|
24112
TGTCATGCTCTATGGTCTTTCTTTCCAGAAAGTTCTTCTCCGATGTCTT
|
C
|
|
24113
ATCACCTTAGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGG
|
G
|
|
24114
ACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTT
|
C
|
|
24115
TAGAGCATTTTCTTGGAAGCAGGATTACCCACACTATTGGTTTACTCCG
|
A
|
|
24116
CATTGACCAATATTCCTCACTGCTGCCTCCCGTAGGAGTTTGGGCCGTG
|
T
|
|
24117
ATCCGCCGCCTTTTCAACGGAGGTCGGTTCGGTCCTCCATGGAATTTTA
|
C
|
|
24118
CCTGTGTCGGTTTACGGTACGGGCGCATGGCAAACGATAGCGGCTTTTC
|
T
|
|
24119
GCCCAAGGGTAGATCACTTGGTTTCGCGTCTACTCCTTCCGACTATACG
|
C
|
|
24120
GGCGGATTTTCCCAAATCCTTCGACTATCAAGTTCTTTGGTAACTCAAA
|
T
|
|
24121
CTTTCGGGGAGTACGAGCTATCTCCGAGTTTGATTGGCCTTTCACTCCT
|
A
|
|
24122
CTCTAGTTAGCCTGCTGCGTCCCTCCTTCACTCAATACTCTAGTACAGG
|
A
|
|
24123
CGCCGTCGATGTGAACTCTTGGGCGAGATCAGCCTGTTATCCCCAGGGT
|
A
|
|
24124
AGTCGTTTCCAACTGTTGTCCCCCACTCCAGGGCAGGTTACTCACGCGT
|
T
|
|
24125
GCATGCTTAAAGTTCGGCGGCTACGGAATTTCAACCGTATGTGCATCGA
|
C
|
|
24126
ATTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCTTATGGTA
|
C
|
|
24127
CGCACAGCCCTGTGTTTTTGTTAAACAGTTGCCTGGACCTATTCTCTGC
|
G
|
|
24128
CATAATTTTATTTTCTTCTCCTACGGGTACTGAGATGTTTCACTTCCCC
|
G
|
|
24129
ACCTTGGGCGGACGAACCTTCCCCAAGAAACCTTAGATTTTCGGCCATT
|
A
|
|
24130
TACTATCAGGTTCGGCTCTCAAGGTGGATTTGCCTGCCTCGATCTGCGC
|
C
|
|
24131
CTGTACATGCAATACCAAGCTCCAGTACCAAACTGGAGTAAAGCTCCAT
|
G
|
|
24132
TGCTTGACCACGGAAAACCACCTCCGCGGCCGGCTCCCATTCCGTGTCA
|
C
|
|
24133
CAGTAACCCGCAAGGCTGCACCTAAATGCATTTCGGGGAGTACGAGCTA
|
T
|
|
24134
AAGCCAACATCCTGGTTGTCTACGCAATTGCACATCCTTTTCCACTTAA
|
C
|
|
24135
CACATCTTACGACGGCAGTCTCGACAGAGTCCCCAGCATCACCTGATGG
|
T
|
|
24136
TTATAGTTACGGCCGCCGTTCACTGGGGCTTCGATTCAATGCTTGCACA
|
T
|
|
24137
CATCTTTACTCGTACTGCAATTTCGCCGAGCTCCTGGTCGAGACAGTGG
|
G
|
|
24138
ACACCGAGCCATGCAGCTCTGTGCGCTTATGCGGTATTAGCAGTCATTT
|
C
|
|
24139
AGGTCCCGCGCTCCCCACCACCGTCCCCGTCAAAGACGGGGTTCGGGAT
|
G
|
|
24140
ATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTA
|
T
|
|
24141
GGAATTTCTCCCCTAGCCACAAGTCATCCGCTAACTTTTCAACGGTAGT
|
C
|
|
24142
GCTCTACCTCCAAGACTCTTACCTTGAGGCTAGCCCTAAAGCTATTTCG
|
G
|
|
24143
TTATAGTTACGGCCGCCGTTTACTGGGGCTTCAATTCAATGCTTCTCTT
|
G
|
|
24144
CTTCAACCTGGACATGGATAGGTCACCCGGTTTCGGGTCTGCACACACT
|
G
|
|
24145
GAGGCTAGCCCTAAAGCTATTTCGAGGAGAACCAGCTATCTCCGGGTTC
|
G
|
|
24146
TGGGCTGTTTCCCTTTCGACTACGGATCTTAGCACTCGCAGTCTGACTG
|
C
|
|
24147
CTCCGGCCTATCCTACACATCGATTGCCCAAATTCAATGTAAAGCTATA
|
G
|
|
24148
CCACTTCACCTAACAACAATGCAAAAAGGGCGTGCCACTGGTAGATGAC
|
A
|
|
24149
ACCCTCAGGTCATCCAGAAGCTTTTCAACGCTTATTGGTTCGGTCCTCC
|
A
|
|
24150
AGTATCCCTTCCTGCAGGTACTGAGATGTTTCACTTCCCTGCGTACCCC
|
C
|
|
24151
ACTTGGTATCCCTTCGGCTCCGCACCTTAAGTGCTTAACCTCGCCAGTA
|
T
|
|
24152
TCGGATACGTGTGTCGTCACACTTAACCTTGCCGGCAAAGGCAACTCGT
|
A
|
|
24153
GGATCACTAACTCCAACTTTCGTTACTGCTCGAACTGTCGCTCTCGCAG
|
T
|
|
24154
CGAACGCCTTAGTATTTTCAACCTGACTACCTGTGTCGGTTTGGGGTAC
|
G
|
|
24155
TTCTGCTTCTGCCCGTACACGTTGCTCCCCTACCCAGAAGTTTCCTTCT
|
G
|
|
24156
TCACGGTACTAGTTCGCTATCGGTCAGACAGGTATATCTAGGCTTACCC
|
C
|
|
24157
ACTTCTTACAAAGCTCCGACCGCTTGTAGGCGCATGGTTTCAGGGACTA
|
T
|
|
24158
TCTTTAAAGGATGGCTGCTTCTGAGCCAACCTCCTAGTTGTCTGGGCAT
|
C
|
|
24159
CCCCATTGGGGCCCACAACACCGCACACACAACCCCTACCAAGTATCAC
|
A
|
|
24160
CTCAACTTCAACCTGCTCATGGCTAGATCACCCGGTTTCGGGTCTGCAA
|
C
|
|
24161
GCATACGCCACACGGCTTATGCTCGCCACCCGCCACTGACTCGCAGACT
|
C
|
|
24162
GTTCGTCTATATGCCCGCACCTCACTGCGCCATGCCGGCAGACATGACC
|
A
|
|
24163
ATCTGGGCTGTTTCCCTTTTGACAATGACATTTATCTGACACTGTCTGA
|
C
|
|
24164
CTATTAGTAGCAGTCAGCTCCATGTGTTACCACACTTCCACCCCTGCCC
|
T
|
|
24165
TTTCACAACTGACTTAAACATCCATCTACGCTCCCTTTAAACCCAATAA
|
A
|
|
24166
CCGTTGAATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC
|
T
|
|
24167
TCCTTAACGAGAGTTCGCTCGCTCACCTGAGGCTACTCGCCTCGACTAC
|
C
|
|
24168
CCACTCCGTCGATGTGAACTCTTGGGAGTGATAAGCCTGTTATCCCCAG
|
G
|
|
24169
CAACAGGATGAAGTTTAGCGGATTTTCTCGGGAGTATGATTACATGCGC
|
T
|
|
24170
GACGGGCTGCGTGCTTGACCACGGAAAACCACCTCCGCGGCCGGCTACC
|
C
|
|
24171
CGGATTTGCCTATGATGCGCGCTGCGTGCTTGACCACGGAAAACCACCT
|
C
|
|
24172
CTGAGTTTGATAAGCTTCGCTAACCTCTCGGCCGCTAGGCTATTCAGTG
|
C
|
|
24173
TGCAGCACCTGTCTCACGGTTCCCGAAGGCACATTCTCATCTCTGAAAA
|
C
|
|
24174
AGGCTAGCCCTAAAGCTATTTCGGGGAGAACCAGCTATCTCCGAGTTCG
|
A
|
|
24175
GACGTCCTATCTCTAGGATTGTCAGAGGATGTCAAGACCTGGTAAGGTT
|
C
|
|
24176
GTTTTGACTACAGGGCTGTTACCTCCTATGGCGGGCCTTTCCAGACCTC
|
T
|
|
24177
CTGGGGCTTCAATTCAGATCTTCGCTAACGCTAAACCCTCCTCTTAACC
|
T
|
|
24178
CCTTAGTATATTCAACCCGACTACGTGTGTCCGTTTACGGTACGGGTAC
|
C
|
|
24179
CTATACATCATCTTACGATTTAGCAGAGAGCTGTGTTTTTGATAAACAG
|
T
|
|
24180
CTAACAATGTCCCCCGACTCGATTCAGAGCCGCAGGTTAGAATTCCAAT
|
A
|
|
24181
TTTGGCCTCTTCCGCGTTCGCTCGCCACTACTTACGGAATCTCAGTTGA
|
T
|
|
24182
CCCGCCAACTGGCTAATCAGACGCGGGTCCATCTTATACCACCGGAGTT
|
T
|
|
24183
GCTACTTGGGACACGCGATCGGAAGACGGCAAGCGTCCAGGTACGGGGC
|
T
|
|
24184
CATCACCGGGGATGAGCTACCTCACTGCGTCCCTCCGCAGCTTGCCTAC
|
T
|
|
24185
ACAACTTAATACCCGATTATTATCCACGCCAGACTCCTCGACTAGTGAG
|
C
|
|
24186
CTCTCAGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTACCCCACCA
|
A
|
|
24187
TCACGTAGTCTGACTGCTGATCATCAATTAGCCGGCATTCAGAGTTTGA
|
T
|
|
24188
TAGGTCACCCGGTTTCGGGTGTACTGCATGCAACTTTACGCCCTTTTCA
|
G
|
|
24189
TACTTTAGTTCGCTCCACATCACGGCTTCGTCTCATGCACAGCGGATTT
|
G
|
|
24190
CTTACGGGGCTTTCACCCTCTCTGGCAGGCTTTCCCAAAAACCTTTCTG
|
C
|
|
24191
GGCCGGGCTTTCGATCCCGTTCTTCTATCCTCTCTCTTGCCATATCATG
|
G
|
|
24192
ACGGCTTCTACTCGTATACAACGCTCCCCTACCACTATAGTTTCCTACA
|
A
|
|
24193
ATCGAGTTTTCTTTCTCTTCCTCCGGCTACTTAGATGTTTCAGTTCACC
|
G
|
|
24194
GCTTTACATACCGAAATACTTCTTCACTCACGCGGCGTCGCTGCATCAG
|
G
|
|
24195
TCCCTTCTGCCTTTGCACTCTTCTAATGGTTTCCGACCATTATGAGGGA
|
A
|
|
24196
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA
|
G
|
|
24197
TGCCAAACCTCCCCGTCGATGTGAACTCTTGGGGGAGATAAGCCTGTTA
|
T
|
|
24198
GCCTGGACCTATTCTCTGCGCCTCACATTACTGTGAGGACCCTTTATCC
|
C
|
|
24199
ACCTTTACACCTGCATCCTATCAACGTCGTAGTCTACAACGACCCTCAG
|
A
|
|
24200
GTATTCATTAACGCTAGAAGCTTTTCTTGGCAGAGTGACATCACTAGCT
|
T
|
|
24201
GCTGTTGGTCCGGATTGTTCTCCTTTAGGACATGGACCTTAGCACCCAT
|
G
|
|
24202
AAAAACCCTCCCCCCCCCCCCTTCCCCTCCGCGGCCACCTTTCCCCCCC
|
C
|
|
24203
CTGTCGGTACCCGATACGGGCCCTCAAGCATCCAGTAGCTCTACCCCCC
|
G
|
|
24204
ATCTACGCATTTCACCGCTACACTAGGAATTCCGCTTACCTCTGTTGCA
|
C
|
|
24205
TCTGTCCCACCTTCGGCGGCTGGCTCCTAAAAGGTTACCTCACCGACTT
|
C
|
|
24206
TGACCAAGGGTAGATCACTTGGTTTCGCGTCTACTCCTTCCGACTAATC
|
G
|
|
24207
TGTGCACTTGCACTCGCCACCCGATTGCCAACCGGGCTGAGCGGACCTT
|
T
|
|
24208
CAGCCTCACTCCCAGGCTGTAAAATATGCCCCTTCGGAGTTTGATAAGG
|
T
|
|
24209
ACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGT
|
T
|
|
24210
CTGTCAAGGTCGACTCTCCCTGCGGATTTGCCTACAGGAATCTACATCT
|
A
|
|
24211
CCTGTGTTTTTGGTAAACAGTCGCTACCCCCTGGCCTGTGCCACCCCCC
|
G
|
|
24212
ATCTGATAGCGTGAGGTCCGAAGATCCCCCACTTTCTCCCTCAGGACGT
|
A
|
|
24213
ACACTTTGGGACCTTAGCCGGTGGTCTGGGCTCTTTCCCTTTTGACTAC
|
C
|
|
24214
CTACAAGGGATCTTACCTGATTGAATCAGTGGGATATCTTATCTTTGGG
|
T
|
|
24215
CTGAAGGGTAACCCCACATAACCAGGGCCAGGTTTCCCCATTCGGACAT
|
C
|
|
24216
TCAGTCCGCGGCGCTGTCACGCCTCCGTCTCCACGTCACTCCTTAAGGT
|
A
|
|
24217
TTAACAAGGGTTCTCCCGTTCGTCTCAGGATTCTCTCCTCGCCCACCTG
|
C
|
|
24218
CTAACATCCTAGTTGTCTGTGCAACCCCACATCCTTTTCCACTTAACAA
|
T
|
|
24219
GATAAATCTTTCCCCCGTAGGGCACATTCGGTATTACTCCCAGTTTCCC
|
G
|
|
24220
GTTTACAATCCGAAGACCTTCTTCCCACACGCGGCGTTGCTGCATCAGG
|
G
|
|
24221
CGGCGCACTGCAGCTACCTGTCTGCGTCACCCCTGTTAACACGCTTGCC
|
T
|
|
24222
ATGAAGCTGGAATCGCTAGTAATCGTATATCAGCAATGATACGGTGAAT
|
A
|
|
24223
CGGATTTGCCTATGGGACGGGCTGCGTGCTTGACCACGGAAAACCACCT
|
C
|
|
24224
GGATGACCCCCTTGCCGAAACAGTGCTCTACCCCCGGAGATGAATTCAC
|
G
|
|
24225
GGTACGGGTAACATATACTATAACTTAGAAGATTTTCTCGGAAGTCGAC
|
T
|
|
24226
CTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCCTCT
|
T
|
|
24227
TCTTACTTCTTGCGAATGGGAGATCTCATCTTGGAGTAGGCTTCGTGCT
|
T
|
|
24228
GTCAAGCTCCCTTATACCTTTACACTCTGCGATTGATTTCCAACCAATC
|
T
|
|
24229
CCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA
|
A
|
|
24230
AAAAGCAGTTTACAACCCATAGGGCCGTCATCCTGCACGCTACTTGGCT
|
G
|
|
24231
TGAGGGCACCTTTAGAAGCCTCCGTTACACTTTTGGAGGCGACCACCCC
|
A
|
|
24232
ACGCTCTAACCTTATGGTAACCGGATTTGCCTGGTAACCAGCCGCTTCG
|
C
|
|
24233
GCTTCCAAGCCAACATCCTAGCTGTCTTAGCAATCTGACTTCGTTAGTT
|
C
|
|
24234
TGGCCGTTCACCCTCTCAGGCCGGCTATGGATCGTCGCCTTGGTAGGCC
|
G
|
|
24235
TGAGCCAACATCCTGGTTGTCTTCGAAATCCCACATCCTTTTCCACTTA
|
A
|
|
24236
CTAGAGAGTATTTAGGGTTAGGAGATGGTCCTCCCAGATTCCGACGAGA
|
T
|
|
24237
GCCTTTCGGCCTCGCGTTAGGTCCCGACTTACCCAGGGCGGACGAACCT
|
T
|
|
24238
GTCAAACTGCCCACCTGACACTGTCTCCCCGCCCGATAAGGGCGGCGGG
|
T
|
|
24239
TGGAGTAAAGCTCCATGGGGTCTTTCCGTCCTGGCGCAGGTAACCAGCA
|
T
|
|
24240
TTTCTTCTCCTACGGGTACTGAGATGTTTCACTTCCCCGCGTAACCCCC
|
A
|
|
24241
ACCAGCTATGGATCGTCGGCTTGGTAGGCCATTACCCCACCAACTACCT
|
A
|
|
24242
GGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT
|
T
|
|
24243
CACCAGTGTCGGTTTGGGGTACGGGCGGCCATAGCCCTCACGCCGAGGC
|
T
|
|
24244
GACGTTCTGAACCCAGCTCGCGTGCCGCTTTAATGGGCGAACAGCCCAA
|
C
|
|
24245
GGTTAGAATTCCAATATCGCAAGGATGGTATCCCAACGGCCTCTCCGCC
|
A
|
|
24246
AGGTTACCCACGCGTTACTCACCCGTCCGCCACTAGAAACAATCTAAAT
|
C
|
|
24247
CAGGTGTCACCCCATATACGTCATCTTTCGATTTAGCATAGAGCTGTGT
|
T
|
|
24248
TCTTTCGGCGAGGGGGTTTCCCACCCCCTTTATCGTTACTTATACCTAC
|
A
|
|
24249
CTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG
|
A
|
|
24250
CCACTTAGTGATGATTTGGGGACCTTAGCTGGCGGTCTGGGTTGTTTCC
|
C
|
|
24251
TCCCCCATTCGGACACCTCCGCTTCTTCGCTTCCTTACAGCTTCACGGA
|
G
|
|
24252
ATAGATCACCCGGTTTCGGGTCTGCCCCCACTGACTCTGGCCCTCTTAA
|
G
|
|
24253
GCCTATCAAACACGTGTTCCACATGCGGGCTTCAGGACCCCGAAGGGCC
|
C
|
|
24254
CCATTTCTGACTGTTATCCCCCTGTATAAGGCAGGTTGCCCACGCGTTA
|
C
|
|
24255
CATCATCTGTATGGCATTCGGAGTTTGATATCCCTTAGTAAGCTTTGAC
|
G
|
|
24256
GTTTGGGGTACGGGCGGCTAAAACCTCGCGCCGATGCTTTTCTAGGCAG
|
C
|
|
24257
GCGATGGCCCTTCCATACGGTACCACCGGATCACTAAGCCCGACTTTCG
|
T
|
|
24258
GAGTTAACCCCGGCGGTCCCCCGTGAGTTCCCACCATAACGTGCTGGCA
|
A
|
|
24259
GGATAATCGGCGGACGGGATTCCCACCCGTCACACGCTACTCATGCCTG
|
C
|
|
24260
TACCTCTTCGTTATGATATGTCCGCAACCCCAATAAAGAAAACTTTATT
|
G
|
|
24261
ACGTGTCCGGCGGTACTCTGGATTCAGCTGGCGGATCTTCTCTTTCGCA
|
T
|
|
24262
TCGAGACCAGACTTCGTTAGACTAACTCAGACAGGATTCCGGGACCTTA
|
G
|
|
24263
TGGCCGTTCAACCTCTCAGTCCGGCTACCAATCGTCGCCTTGGTGGGCC
|
G
|
|
24264
TATAAGTCAAGGCTGCACCTAAATGCATTTCGGGGAGTACGAGCTATCT
|
C
|
|
24265
CTACTGTTTCACCGCGTATACAACGCTCCCCTACCCAGCATGTAAACAT
|
G
|
|
24266
TTATAGTTACGGCCGCCGTTTACTGGGGCTTCAATTCACACCTTCGACA
|
A
|
|
24267
GGATGGACCCCTCACCCAAACAGTGCTCTACCTCCATGATTCTTAATGT
|
C
|
|
24268
TTGGGACCTTAGCTGCGGGTCTGGGCTCTTTCCCTTTTGACTATCCAAC
|
T
|
|
24269
GGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCC
|
T
|
|
24270
TCGCTACTCATTCCGGCATTCTCACTCGTGTACAGTCCACCGCTGCTTT
|
C
|
|
24271
CCTCCCCCCCCCCCCCCCCCCCCCCCCCTTCCCCCCTCTCCTCCCCCTT
|
C
|
|
24272
TAACACCCCATAACAGGTGCCAGGTTTCCCCATTCGGACATCCTCGGAT
|
C
|
|
24273
ACCTCGACACGGACGGTGACAAGCCGGTACCAGAATATCAACTGGTTAC
|
C
|
|
24274
ATAGATCACCCGGTTTCGGGTCTACTCCGGCTGACTCGCTCGCCCTATT
|
C
|
|
24275
TAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTCCC
|
A
|
|
24276
CAGCTTATAGGGTTGCGTACTTCACTACAACCCAACCTTGATGCTTGCA
|
C
|
|
24277
GCTTGGGCCTTTTCACTGCGGCTGACTTATCGCCAGCGCCCCTTCTCCC
|
G
|
|
24278
TGAGGTCGGCTTCACGCTTAGATGCTTTCAGCGTTTATCCGTTCCGCAC
|
T
|
|
24279
CTCCGGGTACTGTCAGGTTCGACTCTCAGGGCGGATTTGCCTACCCCGA
|
T
|
|
24280
GCTTGGGCCTCTTCACTGCGGCTTAATTGCTTAAGCACTCCTTCTCGCT
|
A
|
|
24281
TTTATCCCGAAGTTACAGGGTCAGTTTGCCTAGTTCCTTAACCGTGAAT
|
C
|
|
24282
GTAGTTAGCCGGAGCTTCCTCCTAAAGTACCGTCATTATCGTCCTTTAA
|
G
|
|
24283
TCTTTCGGCGAGGGGGTTTCCCGCCCCCTTTATCGTTACTTATACCTAC
|
A
|
|
24284
GGATGTACTAGCAGCTTTTCTCGCCAGCGTGAACTCACTCGCTTCCCTA
|
C
|
|
24285
TTAGTATCAGTGCTTTATCAGGGGCGCATATACTCGGGTACCAGAATAT
|
C
|
|
24286
GCTTGGCGGCGTCCTACTCTCACAGGGGGAAACCCCCGACTACCATCGG
|
C
|
|
24287
AGATTCACGCAGAATTCCTCGTGCTCCGCGCTACTCAGGATACTACTAT
|
G
|
|
24288
TATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAA
|
A
|
|
24289
TCAATAGGCACGCCACCACACTCTTATGGAGCGGTGACTGCTTGTAAGT
|
C
|
|
24290
CTACTATATTTCGGTCCCTTACGCCCGGGGCAACCATCGCCCGGGATAA
|
C
|
|
24291
TGCCATGACTGCTTGTAAGTCCACGGTTTCAGGTTCTCTTTCACTCCCC
|
T
|
|
24292
TCCATTTGCGCAGCACCAGTAATCATGTTCTTAACATAGTCAGCATGTC
|
C
|
|
24293
TCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCTACTGATCGT
|
C
|
|
24294
TGGCCGTTCAACCTCTCAGTCCGGCTACTGATCGTCGCCTTGGTGGGCC
|
T
|
|
24295
TTATAGTTACGGCCGCCGTTTACCGGGGCTTCAATTCGGAGCTCTCACT
|
C
|
|
24296
TAGTGAAAGGTAGATTTTCTGACCCTTTCGACCTGAACGTACCAACCAG
|
C
|
|
24297
TCTTGGCAGTGTGACATCACTAACTTCGCTACTAAACTTCGCTCCCCAT
|
C
|
|
24298
ACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCT
|
T
|
|
24299
TCGGAGTTTGATATTCTTCGGTAAGCTTTGACGCCCCCTAGGAAATTCA
|
G
|
|
24300
ACCCACCGAGTGGGCGCCCATCAGGTCTCAAGCACATAGCCGGCGGATT
|
T
|
|
24301
TACGGGTGCCGCATGGATAAGTTTAGCGGATTTTCTCGGGAGCATGGTT
|
A
|
|
24302
TTCAAACAACCATCCGGTATTAGCCCCGGTTTCCCGGAGTTATCCCAGT
|
C
|
|
24303
TCCTTAACCACGCTGCATACCATAACTCGCCGGACCATTCTACAAAAGG
|
T
|
|
24304
CCGGCACCGGGCAGGTGTCAGGCTGTATACGTCATCTTTCGAGTTTGCA
|
C
|
|
24305
CAGGAATATTCAGGCTTACCCAACGGTCTGGGCGGATTCGCACGGGGTT
|
C
|
|
24306
TTTATCCCGAAGTTACAGGGTCAGTTTGCCTAGTTCCTTAACCGTGAAT
|
C
|
|
24307
CTTCTGCAATTGCACTCGTCGATTGGTTTCCATCCAATCTGAGCGTACC
|
T
|
|
24308
TCGGTTTGCCCTCTTCCGCGTTCGCTCGCCACTACTTACGGAATCTCGT
|
T
|
|
24309
AAGCTCCATGGGGTCTTTCCGTCTTGTCGCGGGTAACCGGCATCTTCAC
|
C
|
|
24310
CATCGGCCTCACCGTTCGGCTGAGCCTTAGGACCCGACTAACCCTGATC
|
C
|
|
24311
CCTCGCCATACACGCCGCACGGATTTGCCTATGCGACTGGCTGCGTGCT
|
T
|
|
24312
CCTGTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGT
|
C
|
|
24313
TCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGG
|
A
|
|
24314
TTTCACAACACGCTTAAAAGGCGGCCTACGCTCCCTTTAAACCCAATAA
|
A
|
|
24315
CCCCGCGGTACTCTGGATCCTGCTAGCTCTCGCTCCTTTTCGTCTACGT
|
G
|
|
24316
ATCGGTTCACACACTCACCCACCCCAGAAGCATCAAAAACACTCCCAAG
|
A
|
|
24317
TAGAAAGGAGGTGATCCAGCCGCACCTTCCGATACGGCTACCTTGTTAC
|
G
|
|
24318
GCCCATTGTCCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACC
|
G
|
|
24319
TCACCTTTCCCTCACGGTACTGGTTCGCTATCGGTCTCTCGGGAGTATT
|
T
|
|
24320
CGAAGTTACGGGGTCATTTTGCCGAGTTCCTTGACAATGCTTCTCCCGC
|
C
|
|
24321
AGATCCTCTCAAATTTCCTACGCCCGCGACGGATAGGGACCGAACTGTC
|
T
|
|
24322
TCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCTACTGATCGT
|
C
|
|
24323
GGCAACCCAACAACCCACACATCATCATCTTCAGCTACAGGACTCTCAC
|
C
|
|
24324
GCACTATTGCCTTGTCCCGGAGGACGCGGCATACTGTCAGGTTCGAATC
|
A
|
|
24325
CCGTGGCTTTCTGGTTAGGTACCGTCAAGGTACCGCCCTATTCGAACGG
|
T
|
|
24326
ATACTATCAGGTTCGACTCTTATCCCGGATTTGCCTGGGATAATCAACA
|
T
|
|
24327
TAAGTCCTTAACCTTGCTGCATACAATCGCTCGCCGGACCGTTCTACAA
|
A
|
|
24328
ATCTGGGCTGTTTCCCTTTTGACAATGACATTTATCTGACACTGTCTGA
|
C
|
|
24329
AGAGTAACCATAACACAAGGGTAGTATCCCAACAACGCCTCCTCCGAAA
|
C
|
|
24330
TGGACAGGATTCTCACCTGTCTTACGCTACTCATACCGGCATTCTCACT
|
T
|
|
24331
GCCCGGCTACCTTCCTGCGTCACACCTGTTAATACGCTTGGCTCCCCAG
|
T
|
|
24332
GTCAAGCTCCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTC
|
T
|
|
24333
CCCAACCCTTGGAACATACTACAGCCCCAGGTGGCGAAGAGCCGACATC
|
G
|
|
24334
TCTTTCGGCGAGGGGGTTTCCCACCCCCTTTATCGTTACTTATACCTAC
|
A
|
|
24335
GGGTGTTCCCCTTTTGCCCGCGGAACTTATCTCTCGCGGACTGACTCCC
|
A
|
|
24336
ACCCGGTTTCGGGTCTATGGCATACAACTTCTCGCCCTTGTCAGACTCG
|
C
|
|
24337
CTGCCTGGCTTACGCCTACGGGGCTTTCACCCTCTCCGGCGCCGGCATT
|
C
|
|
24338
GCTGCGGGGCTGAGCCCCTTAACCTCGCCGGAAAAAGTAACTCGTAGGT
|
T
|
|
24339
AAGGATGGCTCTCTTCAAATCTCCTGCGCCCGCGACGGATAGGGACCGA
|
A
|
|
24340
CAGGCCCCACAACACCGCACACACAACCCCCGCCGGGTATCACATGCAC
|
A
|
|
24341
CCCCTACGGATCCATGCCTTGGTGGGCCATTACCCCACCAACTAGCTAA
|
T
|
|
24342
ACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG
|
T
|
|
24343
TATCCATCGAAGACTAGGTGGGCCGTTACCCCGCCTACTATCTAATGGA
|
A
|
|
24344
CAGGCGTCAGCTCGTATACGTCATCTTTCGATTTAGCACAAACCTGTGT
|
T
|
|
24345
TGGCCGTTCAACCTCTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCC
|
G
|
|
24346
CCTGTGTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCT
|
C
|
|
24347
ACGCCTTTCGGCCTGACCTTAGCTCCCGACTTACTTGGAGCGGACGAAC
|
C
|
|
24348
GGTCTGGGCTCTTTCCCTTTTGACTGCCCAACTTATCTCGTGCAGTCTG
|
A
|
|
24349
GAATGAATGGCTGCTTCTGAGCCAACATCCTAGTTGTCTTAGAGATCCC
|
A
|
|
24350
CCCCATCATGCCTCAACCTTCACGCCCAGCGGATTTACCTACCAGACAG
|
T
|
|
24351
AAAAGTACGCGGTTCATCATATAAAGATGTTCCACAGCTTGTAAACACA
|
G
|
|
24352
ATCTGAAGTCTTCTCGTTTAACATACAGGACTATTACCTTCTGTGGTGA
|
G
|
|
24353
GGTCACACCCTTTTGAAGTGTCCCTTTGCTTAAATTACAGATGGTTACG
|
G
|
|
24354
CAGCTTATCACGTCTTTCATCGGCTCTTAGTGCCAAGGCATCCACCCTG
|
C
|
|
24355
TTCCATTCGGCACCGCCGGATCACTATTCCCGACTTTCGTCCCTGTTCG
|
A
|
|
24356
TCCAGGTTCGATTGGCATTTCACCCCTACCCACACCTCATCCCCGCACT
|
T
|
|
24357
TACACCTTCTGCGTACATAGAACGCTCTCCTACCATCCCCTAAGGGATC
|
C
|
|
24358
GCTTGCGCTAACCTCTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAG
|
C
|
|
24359
CGCCCGTTAGTACCGGTCGGCTCCACCCCTCGCGGGGCTTCCACCTCCG
|
G
|
|
24360
CTCCGGGACCTTAGACGGCGGTCTGGATTCTTCTCCTCTCGGGGACGGA
|
C
|
|
24361
TGGTTAAGTCCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCAC
|
A
|
|
24362
TAAGTCCTTAACCTTGCTGCATACAATCGCTCGCCGGACCGTTCTACAA
|
A
|
|
24363
ACCGGACTTTCCATTTCCGGCCCATGTTTCCCTCCCGTGTCCCCACAGT
|
T
|
|
24364
CGGCTCCCACCTATGCTACGCAGAAGAATCCGGATATCAATGCCAGACT
|
A
|
|
24365
ACCCCACATCCTTTTCCACTTAACATATATTTGGGGACCTTAGCTGGTG
|
G
|
|
24366
CCACACCACTTCACCTAACAACAACACACAAGCACGATGATGGTAGTCA
|
C
|
|
24367
TCATCCCCGCACTTTTCACGTACGTGTGGTTCGGACCTCCACGACGTCT
|
T
|
|
24368
CCCTTCAAAGCCTCCGACCTATCCTACACATCACGTGCCCAGATTCAAT
|
G
|
|
24369
CTTCACCTAACAACAATGCGCAAGCAGGACGTCAGTAGCCATCCTCATC
|
A
|
|
24370
GGGGTACGGGCGGCAACGCGCCTGACGCCGAAGCTTTTCTCGGCACCAC
|
G
|
|
24371
ATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCA
|
G
|
|
24372
ATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTG
|
A
|
|
24373
GCCGGCTTTCCCAAAGCCGTTCTGCTACCTCTCGCGGATCAATTATGCG
|
G
|
|
24374
ACGCCTTCCGGCCTCACCTTAGCTCCCGACTAACTTGGAGCGGACGAAC
|
C
|
|
24375
ACACCACGCGGCGATACCAACCCGAAGGAAGGAACCACCACGAGGCGGA
|
G
|
|
24376
CCGAACCCCGAGATGCACGCATCTCGGTTTGGCCTCTTTCGCGTTCGCT
|
C
|
|
24377
GGGACTTCATCCTGGCCAAGTGTAGATCACTTGGTTTCGCGTCTACCCC
|
C
|
|
24378
AGCCCTCGACCTATTAGTACTGCCAAGCTGAATGCCTCACGGCACTTAC
|
A
|
|
24379
GGGAGCGGGATTACCTTCACTATCAATCCACCCGAAGGTTTCATGTACT
|
A
|
|
24380
CACGCGGGATTCCACGAGGCCCGCGCTACTTGGGACAACACGATCGGAA
|
G
|
|
24381
CCTACACCCTTCAACCATCTATTCCGTCAGATGGCGGCACTGTCACTAC
|
T
|
|
24382
CCCCGTACCTGTTCTCGATACCAGGTTAGAACCCCGGTCACACAAGAGT
|
G
|
|
24383
GTTTCACGTGTCTGGCCGTACTCTGGATCCTGCGCAGCTCTCTCCGTTT
|
T
|
|
24384
TTCCCGCTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACC
|
G
|
|
24385
GCACTCCCACAGCTTGTAGACACAGGGTTTCAGGTTCTCTTTCACTCCC
|
C
|
|
24386
CCTGGCCAAGGGTAGATCACTTGGTTTCGCGTCTGCCACTGCCGACTAT
|
A
|
|
24387
CCGCGAGGGACCTCACCTACATATCAGCGTGCCTTCTCCCGAAGTTACG
|
G
|
|
24388
AAGCTCCATGGGGTCTTTCCGTCTTGCCGCAGGTAACCGGCATCTTCAC
|
C
|
|
24389
CGTCGGCTTGGTGGGCCGTTACCTCACCAACTACCTAATCCAACGCGGG
|
T
|
|
24390
GCTCCCACCTATCCTGTACATGCAATACCAAGCTCCAGTACCAAACTGG
|
A
|
|
24391
ACCGGACTTTCCATTTCCGGCCCATGTTTCCCTCCCGTGTCCCCACAGT
|
T
|
|
24392
CAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATA
|
C
|
|
24393
GGTCCCGGCAGATTCGCGCAGGATTCCTCGTGTCCCGCGTTACTCAGGA
|
T
|
|
24394
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA
|
A
|
|
24395
GGGGGCGGGGAGCGGGGCGTGGGCGGGAGGAGGGGAGGAGGCGTGGGGG
|
G
|
|
24396
CACGAGGCCCGCGCTACTTGGGACACGCGATCGGGAGACGGCAAGCGTC
|
C
|
|
24397
CGTTTATCCCCTCCCTACTTAGCTACCCAGCGATGCTCTTGGCAGAACA
|
A
|
|
24398
CCTCTTAACCTTCCGGCACCGGGCAGGCGTCAGAGCGTATACAGCGGCT
|
T
|
|
24399
ACCTTGGGCGGACGAACCTTCCCCAAGAAACCTTAGATTTTCGGCCATT
|
A
|
|
24400
TTCGTTCGCCACTACTAGCAGAATCATAATTTTATTTTCTTCTCCTACG
|
G
|
|
24401
GTTTCTCGCATGCCTCTCGCTACTCATACCGGCATTCTCTCTTGTGCAG
|
T
|
|
24402
CCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTC
|
A
|
|
24403
CTGTTATCCCCAGGGTAGCTTTTATCCGTTGAGCGACGGCATTTCCATT
|
C
|
|
24404
CAACAATATATGGAACACCTACCTGGCGAGACAATAGAATGTGTTCCCT
|
C
|
|
24405
TTATAGTTACGGCCGCCGTTTACTGGGGCTTCAATTCAATGCTTCTCTT
|
G
|
|
24406
ACAACAGAGCTTTACGATCCGAAAACCTTCATCACTCACGCGGCGTTGC
|
T
|
|
24407
CCCGTTCCACGGGTTAGAATCCAAACAAATAAAGGGTCGTATTTCAACA
|
G
|
|
24408
CCCCCTTCCCCCCTCTCCTCCCCCTTCCCCCTTTCGCGCCCCCTTTTCC
|
C
|
|
24409
TGGTGTTCCAACCAATTCGGCTTGGGGGGATGGATCTTAAAAACTGGTC
|
C
|
|
24410
CTCGTGTCCCGCCGTACTCAGGATCCTGCTTGGCATCAAGTGAATTTCA
|
A
|
|
24411
AGCTTCTACACCCTTCAACCATCTATTCCGTCAGATGGCGGCACTGTCA
|
C
|
|
24412
CCGATTAGTACCAGTCAACTCCGTACATCACTGCACTTCCATCCCTGGC
|
C
|
|
24413
CGCTTGAACCACACATCAGGCCCCACGGCTTGCCACCATGTTAACCCGA
|
A
|
|
24414
TGGCGAGACAATAGAATGTGTTCCCTCGTTTGTGGCATAGGACCATCAG
|
C
|
|
24415
CGTCCATCCCGGTCCTCTCGTACTAGGGACAGCTCCTCTCAAATATCCT
|
G
|
|
24416
TCGAGGTGCCAAACCTCCCCGTCGATGTGAACTCTTGGGGGAGATAAGC
|
C
|
|
24417
CTTAACAACTTAACCTCGCTGCACACAGTAACTCGCCGGCCCGTTCTAC
|
A
|
|
24418
GTCAACAGGTAGTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACAC
|
G
|
|
24419
AGGCACGCCGTCACACATTGCTGTGCTCCGACCGCTTGTAGGCGTATGG
|
T
|
|
24420
TCCCTTTCCCCCTTCCCCCCCCCCCCCCCCCCCCCCCCCTTTCCCCCCC
|
C
|
|
24421
AACCATGACTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTC
|
A
|
|
24422
TGCCATTACACTCTATGAGACCGGTTACCAATCGGTCCGAAGGGCACCT
|
T
|
|
24423
GATTGGAATTTCTCCGCTACCCACACCTCATCCGCTACCATTTCAACGG
|
G
|
|
24424
TTCTCGTGTCCCGCGGTACTCTGGATCCTGCTCAGTCTGCTCTGTTTTC
|
G
|
|
24425
GTAAACCCCCACAACAGCTATGAATTCACTGAAGGGTAACACCCCATAA
|
C
|
|
24426
TCCCGAAGTTACAGGGTCAATTTGCCTAGTTCCTTAACCGTGAATCACT
|
C
|
|
24427
CCCCCGACGGGTATCACACGCGCAAGGTTTGGCCATCATCCGCTTTCGC
|
T
|
|
24428
CCCTTGTCTCAGTGCCCATCTCCGGGCTCCTCCTTCCAGAGCCCGTACC
|
C
|
|
24429
TCAGACTTGCTCTCGCTGCGGCTTCACACCTTAAGTGCTTAACCTCGCC
|
G
|
|
24430
CTCCATTCGGAAATCCACGGATCAATGCCTACTTACGGCTCCCCGTGGC
|
T
|
|
24431
TTTTACGGTTGAGCCGCAAACTTTCACAACTGACTTAACAACCCGCCTA
|
C
|
|
24432
CGGTTTAGGCTCTTCCGCGTTCGCTCGCCGCTACTTACGGAATCGAGTT
|
T
|
|
24433
CTTCACTATATACTCTAGTACAGGAATATCAACCTGTTGGCCATCGGAT
|
A
|
|
24434
TGTTTCAGTTCACTGCGTCTTCCTTCTCATAACCTTAACAGTTATGGAT
|
A
|
|
24435
GACGGAGCTTATCCCCCGCCGACTCACTGCCGGGATACGCGTCACGGGT
|
A
|
|
24436
CCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCTTTAATG
|
G
|
|
24437
GACGGTGACAAGCCGGTACCAGAATATCAACTGGTTACCCATCGACTAC
|
G
|
|
24438
GATGCGCATTCGGAGTTTGTCAAGACTTGATAGGCGGTGAAGCCCTCGC
|
A
|
|
24439
TAGGTGAGCCGTTACCCCACCTACTAGCTAATCCCATCTGGGCACATCC
|
G
|
|
24440
TGGTCCCCGCTCATTCCATCAAGGTTTCTCGTGTCTCGATGTACTCTGG
|
A
|
|
24441
ATGCTCCCCTACCGATACTTTTTAATGCTATCCCGCGCCTTCGGTACCT
|
G
|
|
24442
TTACCTTTACTTCAACCTGACCATGGGTAGGTCACCCGGTTTCGGGTCG
|
A
|
|
24443
GTAGTATTTAGCCTTGGAGGATGGTCCCTCCTGCTTCCCACAGGGTTTC
|
A
|
|
24444
GATTTCCAACCATTCTGAGGGAACCTTTGGGCGCCTCCGTTACCTTTTA
|
G
|
|
24445
ATCCCTTCCGGGCTTGGCTACTCGGCCGTAGACTTGGCAGTCTAACCGA
|
T
|
|
24446
GATGCGCATTCGGAGTTTGTCAAGACTTGATAGGCGGTGAAGCCCTCGC
|
A
|
|
24447
GTAATCGCCTTGGTGGGCCATTACCCCACCAACAAGCTGATAGGCCGCA
|
G
|
|
24448
ACCCTCAGGTCATCCAGAAGCTTTTCAACGCTTATTGGTTCGGTCCTCC
|
A
|
|
24449
AGCTCCATGGGGTCTTTCCGTCTAGTTGCGGGTAACCTGCATTTTCACA
|
G
|
|
24450
CGTGGGGATTAAGTTTAGCGGATTTTCTCGGGAGTATGATTACGTGCGC
|
T
|
|
24451
TATTTTGGGACCTTAACTGGCGGTCTGGGCTGTTTCCCTCTTGACCATG
|
G
|
|
24452
TAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACG
|
C
|
|
24453
GACGGCCCAGAGACCTGCCTTCGCCATCGGTGTTCTTCCCGATATCTAC
|
A
|
|
24454
TCACACGGGATTCCACGAGTCCCGCGCTACTTGGGAGACACGATCCGGA
|
G
|
|
24455
AGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCAC
|
G
|
|
24456
TTTGGCCTCTTCCGCGTTCGCTCGCCACTACTAGCGGAATCTCGGTTGA
|
T
|
|
24457
CTGCTTCCAAGCCAACATCCTAGCTGTCTTAGCAGTCAGACTTCGTTAG
|
T
|
|
24458
CTGGGGCTTCAATTCACACCTTCGCTTACGCTAAGCGCTCCTCTTAACC
|
T
|
|
24459
GTTTGGGCTTCTCCCCTTTCGCTCGCCGCTACTCAGGGAATCACTGTTG
|
T
|
|
24460
ACAATCCACACCGAATGCCAATACCAAGGTATAGTAAAGGTCCCGGGGT
|
C
|
|
24461
CAGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATACGGTACCACC
|
G
|
|
24462
ATAGGCGGTGAAGCCCTCTTGACCTATCGGTCGCTCTACCTCTCACGGT
|
G
|
|
24463
GCCATGCAGATTCTCACTGCATTCGCGCTACTCATTCCGGCATTCTCAC
|
T
|
|
24464
CGGTACGCCGCCGGTACGGGAATATCCACCCGTTCATCCATTCGACTAC
|
G
|
|
24465
GCACTCCACAGCTCCTTCCGGTACTGCTTCTTCGCGTTAAGAATGCTCC
|
T
|
|
24466
CGTTCACTCTTCCTTGGCTCCTACCTATCCTGTACATGTGTAACAGATA
|
C
|
|
24467
CCCCTGACCTGATTCAAGGCCACAGGTTAGAATTTCAGCACTTCAAGAG
|
T
|
|
24468
CTACCCAGCAATGCCTTTGGCAAGACAACTGGTACACCAGCGGTAAGTC
|
C
|
|
24469
CCAGCACCGGGCAGGCGTCACCCCCTATACTTCATCTTACGATTTCGCA
|
G
|
|
24470
ATTCCTCACTGCTGCCTCCCGTAGGAGTTTGGACCGTGTCTCAGTCCCA
|
A
|
|
24471
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCC
|
C
|
|
24472
CTCTCAACGATGACGTCTCCTCTTAACCTTCCAGCACCGGGCAGGTGTC
|
A
|
|
24473
ATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTA
|
A
|
|
24474
GCGATGGACTTTCACACCGGACGCGACGAGCCGCCTACGAGCCCTTTAC
|
G
|
|
24475
CCCACACCGGATATGGACCGAACTGTCTCACGACGTTCTGAACCCAGCT
|
C
|
|
24476
GAATGAATGGCTGCTTCTGAGCCAACATCCTAGTTGTCTTAGAGATCCC
|
A
|
|
24477
TCCCCGGAGTACCTTTTATCCTTTGAGCGATGTCCCTTCCATACGGAAA
|
C
|
|
24478
GTAAAGCCACCTTATACCCTTGCATTCTACAGGAGATTTCTGACCTCCT
|
T
|
|
24479
TCCGCCTGCGCACCCTTTAAACCCAATAAATCCGGATAACGCTCGTATC
|
C
|
|
24480
AGGAAGTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACACGATT
|
C
|
|
24481
GTGTAGGATTCTCACCTACATCTCGCTACTCACACCGGCATTCTCACTT
|
C
|
|
24482
GAACTGAGACCGGTTTTCAGGGATCCGCTCCATGTCGCCATGTCGCATC
|
C
|
|
24483
TTCCTGAAGTTGATTCTTCGGGTTAGACAGCCAAACTTCTCAGGGTGGT
|
A
|
|
24484
CGGTACTGGTACGCTATCGGTCAGACAGGTATGCTTAGACTTACGCCAC
|
G
|
|
24485
GTTTCCCCTCGACTTGCATGTGTTAAGCCTGTAGCTAGCGTTCATCCTG
|
A
|
|
24486
CGAAGTTACGGGGTCATTTTGCCGAGTTCCTTGACAATGCTTCTCCCGC
|
C
|
|
24487
CTTGGGAATGATCAGCCTGTTATCCCCGGGGTACCTTTTATCCGTTGAG
|
C
|
|
24488
GTCTATAAGTACTTCGATTTTTGCAAGTCCGAACCCCGAACGTCCGTAG
|
A
|
|
24489
CACCTTTCCTTCACAGTACTGGTTCACTATCGGTCTCTCGGGAGTATTT
|
A
|
|
24490
CCGGGAATTCCAGTCTCCCCTACCGCACTCCAGCCCGCCCGTACCCGGC
|
G
|
|
24491
ACAGCTTTTCTCGCCATCTTCCATCTCGGACTTCGGTACTAATTTCCCT
|
C
|
|
24492
TCTTTCGGCGAGGGGGGTTCCCGCCCCCTTTATCGTTACTTATACCTAC
|
A
|
|
24493
TGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGA
|
T
|
|
24494
CTTTCGTCTCTGATCGAGTTGTCACTCTCGCAGTCAGGCACCCTTCTGC
|
C
|
|
24495
GATACTACAATTTCACTGAGCTCTTGGTTGAGACAGCGTCCGGATCATT
|
A
|
|
24496
GATGTTTCAGTTCAGGCGGTTCCCTCAATACACCTATTTTAAATTTCAG
|
T
|
|
24497
AAAAAAAAACAAAAAAAAAAACCCTCCCCCCCCCCCCTTCCCCTCCGCG
|
G
|
|
24498
GCCCTGTTAAGACTTGGTATCCCTTCGGCTCCGCACCTTAAGTGCTTAA
|
C
|
|
24499
ACCACGAATTCCGCCTGCCTCAACTGCACTCAAGATATCCAGTATCAAC
|
T
|
|
24500
GAGTTTTTCACACTGTGCCATGCAGCACTGTGCGCTTATGCGGTATTAG
|
C
|
|
24501
TGCCTAGTTCCTTAACCATGAATCTCTCAACGCCTCAGTATGTTCTACC
|
C
|
|
24502
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCGCCGTGGCTGATGCGC
|
G
|
|
24503
TTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT
|
G
|
|
24504
CGCTTAACGCGTTAGCTCCGACACGGAACACGTGGAACGTGCCCCACAT
|
C
|
|
24505
ACACGAGCCGAAACCCGTGTCTCTCAGACTCCCACCTATCCTGTGCATC
|
A
|
|
24506
ACTCGATTTCTCTTCGGCTCCACACCTTAAGTGCTTAACCTTGCCGGCA
|
C
|
|
24507
TGAACCCGCCCCGAAGGGAAACGCCATCTCTGGCGTCGTCGGGAACATG
|
T
|
|
DESCRIPTION OF THE EMBODIMENTS
This application discloses method for enriching viral molecules from a nucleic acid sample, particularly coronavirus molecules. In some embodiments, the viral molecules are viral RNA molecules. In some embodiments, the viral molecules are genomic viral DNA or RNA molecules. In some embodiments, solid supports can be prepared for enriching desired library fragments or depleting unwanted library fragments, wherein oligonucleotides are immobilized to the solid support. In some embodiments, the solid support is a flowcell.
Also disclosed herein are compositions comprising a probe set comprising at least two DNA probes complementary to at least one target viral nucleic acid molecules in a nucleic acid sample.
Disclosed herein are also kits for depleting or enriching libraries. In some embodiments, the kit comprises probe compositions disclosed herein and instructions for using the probe set. Such a kit may further comprise reagents for preparing a cDNA library from RNA, such as reagents for a stranded method of cDNA preparation from a sample comprising RNA, as described below.
I. Target and Off-Target Nucleic Acids
A. Coronavirus Targets
Described herein are methods for enriching viral molecules from a nucleic acid sample. In some embodiments, the viral molecule is a coronavirus molecule. In some embodiments, at least one coronavirus molecule is of the Alphacoronavirus, Betacoronavirus, Deltacoronavirus, Gammacoronavirus, and/or Bafinivirus genus.
As used herein, the term “nucleic acid” is intended to be consistent with its use in the art and includes naturally occurring nucleic acids or functional analogs thereof. Particularly useful functional analogs are capable of hybridizing to a nucleic acid in a sequence specific fashion or capable of being used as a template for replication of a particular nucleotide sequence. Naturally occurring nucleic acids generally have a backbone containing phosphodiester bonds. An analog structure can have an alternate backbone linkage including any of a variety of those known in the art. Naturally occurring nucleic acids generally have a deoxyribose sugar (e.g., found in deoxyribonucleic acid (DNA)) or a ribose sugar (e.g., found in ribonucleic acid (RNA)). A nucleic acid can contain any of a variety of analogs of these sugar moieties that are known in the art. A nucleic acid can include native or non-native bases. In this regard, a native deoxyribonucleic acid can have one or more bases selected from the group consisting of adenine, thymine, cytosine or guanine and a ribonucleic acid can have one or more bases selected from the group consisting of uracil, adenine, cytosine, or guanine. Useful non-native bases that can be included in a nucleic acid are known in the art. The term “target,” when used in reference to a nucleic acid, is intended as a semantic identifier for the nucleic acid in the context of a method or composition set forth herein and does not necessarily limit the structure or function of the nucleic acid beyond what is otherwise explicitly indicated.
In some embodiments, the present methods decrease library preparation costs and hands-on-time, as compared to prior art methods of enrichment, followed by library preparation.
As used herein, “desired RNA” or “a desired RNA sequence” refers to any RNA that a user wants to analyze. As used herein, a desired RNA includes the complement of a desired RNA sequence. Desired RNA may be RNA from which a user would like to collect sequencing data, after cDNA and library preparation. In some instances, the desired RNA is mRNA (or messenger RNA). In some instances, the desired RNA is a portion of the mRNA in a sample. For example, a user may want to analyze RNA transcribed from cancer-related genes, and thus this is the desired RNA.
As used herein, “desired library fragments” refers to library fragments prepared from cDNA prepared from desired RNA.
In some embodiments, the desired RNA sequence is a coronavirus sequence.
Also disclosed herein are compositions comprising a library fragment bound to an immobilized oligonucleotide on a solid support. In some embodiments, a single-stranded library fragment comprising cDNA prepared from a sample comprising RNA is hybridized to a solid support comprising immobilized oligonucleotides. In some embodiments, the cDNA comprised in the composition is complementary to RNA comprised in the sample.
B. Off Target RNA
Also described herein are methods for depleting off-target RNA molecules from a nucleic acid sample.
As used herein, “off-target RNA,” “an off-target RNA sequence”, “unwanted RNA,” or “an unwanted RNA sequence” refers to any RNA that a user does not wish to analyze. As used herein, an unwanted RNA includes the complement of an unwanted RNA sequence. When RNA is converted into cDNA and this cDNA is prepared into a library, a user would sequence library fragments that were prepared from all RNA transcripts in the absence of depletion. Methods described herein for depleting library fragments prepared from unwanted RNA can thus save the user time and consumables related to sequencing and analyzing sequencing data prepared from unwanted RNA. In some embodiments, off-target RNA relates to small non-coding RNA (sncRNA). In some embodiments, the off-target RNA comprises sncRNA with MALAT 1. In some embodiments, off-target RNA comprises at least one small noncoding RNA chosen from RN7SK, RN7SL1, RN7SL2, RN7SL5P, RPPH1, SNORD3A. In some embodiments the off-target RNA is not MALAT1.Small noncoding RNAs are highly abundant as reads during the sequencing process and can lead to noise when analyzing sequencing data. MALAT1 is also highly abundant in the genome. MALAT1 is a highly conserved large, infrequently spliced non-coding RNA which is highly expressed in the nucleus. Trying to remove these reads after sequencing results in wasted sequencing.
As used herein, “off-target RNA,” “unwanted RNA” or “unwanted RNA sequence” also includes fragments of such RNA. For example, an unwanted RNA may comprise part of the sequence of an unwanted RNA. In some embodiments, unwanted RNA sequence is from human, rat, mouse, or bacteria. In some embodiments, the bacteria are Archaca species, E. Coli, or B. subtilis.
As used herein, “off-target library fragments” or “unwanted library fragments” also includes library fragments prepared from cDNA prepared from unwanted RNA.
Also described herein are compositions comprising a probe set comprising at least two DNA probes complementary to discontiguous sequences at least 5, or at least 10, or 15 bases apart along the full length of at least one off-target RNA molecule in a nucleic acid sample and a ribonuclease capable of degrading RNA in a DNA: RNA hybrid, wherein the off-target RNA comprises at least one small noncoding RNA chosen from RN7SK, RN7SL1, RN7SL2, RN7SL5P, RPPH1, SNORD3A
In some embodiments, the off-target RNA is high-abundance RNA. High-abundance RNA is RNA that is very abundant in many samples and which users do not wish to sequence, but it may or may not be present in a given sample. In some embodiments, the high-abundance RNA sequence is a ribosomal RNA (rRNA) sequence. Exemplary high-abundance RNAs are disclosed in WO2021/127191 and WO 2020/132304, each of which is incorporated by reference herein in its entirety.
In some embodiments, the high-abundance RNA sequences are the most abundant RNA sequences determined to be in a sample. In some embodiments, the high-abundance RNA sequences are the most abundant RNA sequences across a plurality of samples even though they may not be the most abundant in a given sample. In some embodiments, a user utilizes a method of determining the most abundant RNA sequences in a sample, as described herein.
In a given sample, the most abundant sequences are the 100 most abundant sequences. In some embodiments, in addition to depleting the 100 most abundant sequences, the method also is capable of depleting the 1,000 most abundant sequences, or the 10,000 most abundant sequences in a sample. In some embodiments, the off-target RNA sequence comprises a sequence with homology of at least 90%, at least 95%, or at least 99% to a most abundant sequence in a sample comprising RNA. In some embodiments, the off-target RNA sequence comprises a sequence with homology of at least 90%, at least 95%, or at least 99% to a most abundant sequence in a sample comprising RNA, wherein the most abundant sequences comprise the 100 most abundant sequences. In some embodiments, homology is measured against the 1,000 most abundant sequences, or the 10,000 most abundant sequences.
In some embodiments, the high-abundance RNA sequences are comprised in RNA known to be highly abundant in a range of samples.
In some embodiments, the off-target RNA sequence is globin mRNA or 28S, 23S, 18S, 5.8S, 5S, 16S, 12S, HBA-A1, HBA-A2, HBB, HBB-B1, HBB-B2, HBG1, or HBG2 RNA, or a fragment thereof.
In some embodiments, the off-target RNA sequence is 28S, 18S, 5.8S, 5S, 16S, or 12S RNA from humans, or a fragment thereof. In some embodiments, the off-target RNA sequence is rat 16S, rat 28S, mouse 16S, or mouse 28S RNA.
In some embodiments, the off-target RNA sequence is comprised in mRNA related to one or more “housekeeping” genes. For example, a housekeeping gene may be one that is commonly expressed in a sample from a tumor or other oncology-related sample, but that is not implicated in tumor genesis or progression. Housekeeping genes are typically constitutive genes that are required for the maintenance of basal cellular functions that are essential for the existence of a cell, regardless of its specific role in the tissue or organism. In some embodiments, the off-target RNA sequence is comprised in 23S, 16S, or 5S RNA from Gram-positive or Gram-negative bacteria.
II. Compositions
Described herein are compositions comprising a probe set comprising at least one DNA probe comprising at least one sequence of SEQ ID NOs: 1-22909.
Also described herein are compositions comprising a probe set comprising at least two DNA probes complementary to at least one target coronavirus nucleic acid molecules in a nucleic acid sample wherein the target coronavirus nucleic comprises at least one coronavirus molecule selected from Table 2.
In some embodiments, the one or more target coronavirus nucleic acids are coronavirus RNA molecules. In some embodiments, the one or more target coronavirus nucleic acids are genomic coronavirus RNA molecules.
In some embodiments, the probe set further comprises at least two DNA probes that each hybridize to at least one target coronavirus molecule of the Alphacoronavirus, Betacoronavirus, Deltacoronavirus, Gammacoronavirus, and/or Bafinivirus genus.
In some embodiments, the probe set further comprises at least two DNA probes that each hybridize to at least one target coronavirus molecule selected from Table 2.
Also described herein are compositions comprising a probe set comprising at least one DNA probe comprising at least one sequence of SEQ ID NOs: 1-22909. In some embodiments, the composition comprises 2 or more, 5 or more, 10 or more, 25 or more, 50 or more, 100 or more, 200 or more, 300 or more, 400 or more, 500 or more, 600 or more, 700 or more, 800 or more, 900 or more, 1000 or more, 1100 or more, or 22909sequences selected from SEQ ID NOs: 1-22909. In some embodiments, the at least one DNA probe comprises 500 or more, 600 or more, 700 or more, 800 or more, 900 or more, 1000 or more, 1100 or more, or 22909sequences selected from SEQ ID NOs: 1-22909.
In some embodiments, the composition comprises at least 5, at least at least 10, at least 50, at least 100, at least 250, at least 500, at least 750, at least 1000, at least 1500, or at least 2000 sequences of SEQ ID NOs: 1-22909. In some embodiments, the composition comprises two or more, five or more, 10 or more, or 25 or more sequences selected from SEQ ID NOs: 1-22909.
In some embodiments the probe set comprises any one or more of SEQ ID NOs: 22910-24507.
In some embodiments the probe set is biotinylated.
III. Methods of Use
A. Methods of Enriching for Viral Nucleic Acids
Described herein are methods of enriching a sample for one or more target viral nucleic acids.
In some embodiments, the present methods decrease library preparation costs and hands-on-time, as compared to prior art methods of enriching for vial nucleic acids, followed by library preparation.
In some embodiments, the method comprises providing any of the compositions described herein, in Section II (Compositions) above.
In some embodiments, the method comprises providing a probe set comprising at least two nucleic acid probes complementary to one or more target viral nucleic acids, wherein the probe set comprises at least two of SEQ ID NOs: 1-22909; allowing the probes in the probe set to hybridize to the target viral nucleic acids; and enriching the sample for the one or more target viral nucleic acids by amplifying the target viral nucleic acids and/or separating the target viral nucleic acids from the sample.
Also described herein are methods of enriching a sample for one or more target coronavirus nucleic acids. In some embodiments, the present methods detect or enrich for new or unknown viral pathogens, including coronaviruses, or new or unknown strains of viral pathogens, including coronaviruses. This may include analysis of patient samples. In some embodiments, the present methods detect co-infections with one or more additional pathogens, including viruses or bacteria. In some embodiments, the present methods detect or enrich for specific viral pathogen strains. In some embodiments, the present methods can be used to perform strain typing and/or strain characterization for monitoring viral pathogen evolution and epidemiology (e.g., coronavirus evolution and epidemiology). In some embodiments, the present methods detect or enrich for viral nucleic acids that exhibit resistance. Resistance can include resistance to anti-viral therapies (whether small molecule therapy or other therapies including treatment with antibodies (including antigen-binding fragments thereof or other biologics with CDRs responsible for specific binding), viral entry inhibitors, viral assembly inhibitors, viral DNA and RNA polymerase inhibitors, viral reverse transcriptase inhibitors, viral protease inhibitors, viral integrase inhibitors, and inhibitors of viral shedding. In some embodiments, the present methods are used to identify hospital-associated viral infections (e.g., hospital-associated coronavirus infections). As used herein, a hospital-associated viral infection refers to an infection whose development spread through and/or is favored by a hospital environment, nursing home, rehabilitation facility, group home, residential facility, medical office, clinic, or other clinical settings. This infection is spread to a subject in the clinical setting by a number of means, for example through contaminated equipment, bed linens, or air droplets. In some embodiments, the present methods are used for viral resequencing. In some embodiments, resequencing allows for testing for known mutations or scanning for one or more mutations in a given target region. Such methods may be used in a panel used for detection of and/or typing of viral pathogens (e.g., coronaviruses).
In some embodiments, the method comprises providing a probe set comprising at least two nucleic acid probes complementary to one or more target coronavirus nucleic acids, wherein the nucleic acid probes are affixed to a support; capturing one or more target coronavirus nucleic acids on a support; using the one or more captured target coronavirus nucleic acids as a template strand to produce one or more nucleic acid duplexes immobilized on the support, wherein the at least one target coronavirus nucleic acids hybridize to one or more probes in a probe set on the support; contacting a transposase and transposon with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand; contacting the one or more tagged nucleic acid duplexes with a nucleic acid modifying enzyme under conditions to extend the 3′ end of the immobilized strand to the 5′ end of the template strand to produce one or more end-extended tagged nucleic acid duplexes; amplifying the one or more end-extended tagged nucleic acid duplexes to produce a plurality of tagged nucleic acid strands; contacting the plurality of tagged nucleic acid strands with a probe set to create an enriched library; and amplifying the enriched library. A wide variety of solid supports may be used to immobilize oligonucleotides for depleting or enriching as described herein, including those described in WO 2014/108810, which is incorporated in its entirety herein.
The composition and geometry of the solid support can vary with its use. In some embodiments, the solid support is a planar structure such as a slide, chip, microchip and/or array. As such, the surface of a substrate can be in the form of a planar layer. In some embodiments, the solid support comprises one or more surfaces of a flowcell. The term “flowcell” as used herein refers to a chamber comprising a solid surface across which one or more fluid reagents can be flowed. Examples of flowcells and related fluidic systems and detection platforms that can be readily used in the methods of the present disclosure are described, for example, in Bentley et al., Nature 456:53-59 (2008), WO 04/018497; U.S. Pat. No. 7,057,026; WO 91/06678; WO 07/123744; U.S. Pat. Nos. 7,329,492; 7,211,414; 7,315,019; 7,405,281, and U.S. 2008/0108082, each of which is incorporated herein by reference.
In some embodiments, a flowcell is comprised within an apparatus or device for sequencing nucleic acids, which may be referred to as a sequencer. In some embodiments, a sequence may also comprise reservoirs for collection of samples or tubing (such as for collecting samples in a reservoir of for exiting of waste). In some embodiments, one or more reservoirs are separate from the flowcell and are comprised in the sequencer. In some embodiments, modifications are made to standard sequencers to improve fluidics system recipes and/or hardware for use of reservoirs in the present methods.
As used herein, a “flowcell” may comprise a flowcell-like device that is not intended to be imaged. While standard flowcells used for imaging may be employed in the present methods, flowcells can also be engineered differently than flowcells intended for imaging. In some embodiments, a flowcell may have a high density of immobilized oligonucleotides, wherein imaging infrastructure would have difficulty separating out into different bridge-amplified clusters associated with different immobilized oligonucleotides. In some embodiments, a high density of immobilized oligonucleotides improves hybridization efficiency. In some embodiments, standard clear glass may be used in a flowcell. In other embodiments, hard plastic may be used in the flowcell. Use of glass in a flowcell may allow use of a standard flowcell without further optimization, whereas use of hard plastic may reduce the cost of manufacturing the flowcell and/or improve stability of a flowcell. Depending on the advantages desired, different materials may be used. In some embodiments, immobilized oligonucleotides are embedded in a substrate other than that of a standard flowcell (i.e., embedded in a substrate other than PAZAM) to improve immobilization of oligonucleotides of longer length.
B. Methods of Supplementing a Probe Set for Use in Enriching for Viral Nucleic Acids
Also described herein are methods of supplementing a probe set for use in enriching for viral nucleic acid molecules from a nucleic acid sample.
In some embodiments, the methods of enriching for viral nucleic acids described herein can be supplemented with or used in conjunction with other enrichment panels. In some embodiments, the method also targets genitourinary pathogens, Antimicrobial Resistance (AMR) markers, respiratory viruses, respiratory pathogens (e.g., viruses, bacteria, fungi, and/or parasites), and/or exonic content. In some embodiments, the method is used with, supplemented with, or used in conjunction with the Urinary Pathogen ID/AMR Panel or Enrichment Kit (UPIP; Illumina). In some embodiments, the method is used with, supplemented with, or used in conjunction with the Respiratory Virus Oligos Panel or Enrichment Kit (RVOP; Illumina). In some embodiments, the method is used with the Illumina Exome Panel (Illumina). In some embodiments, the method is used with, supplemented with, or used in conjunction with the Virus Surveillance Panel or Enrichment Kit (VSP; Illumina) In some embodiments, the method is used with, supplemented with, or used in conjunction with the Respiratory Pathogen ID/Antimicrobial Resistance (AMR) Panel or Enrichment Kit (Illumina). In some embodiments, the method is used with, supplemented with, or used in conjunction with the Pan-CoV Panel or Enrichment Kit (Illumina). In some embodiments, the method is supplemented with or used in conjunction with the Illumina Exome Panel (Illumina). In some embodiments, the method targets and enriches for coding RNA sequences. In some embodiments, the method is used with the Illumina RNA Prep with Enrichment (Illumina).
Examples of supplemental probe sets that can be readily used in the methods of the present disclosure are described, for example, in U.S. Provisional Application No. 63/250,563, filed Sep. 30, 2021, U.S. Provisional Application No. 63/351,170, filed Jun. 10, 2022, and U.S. Provisional Application No. 63/378,610, filed Oct. 6, 2022, each of which is incorporated by reference herein in its entirety.
In some embodiments the method comprises depleting unwanted nucleic acid molecules from a nucleic acid sample.
In some embodiments, the depleting unwanted nucleic acid molecules comprises depleting unwanted cDNA library fragments from a library of cDNA fragments prepared from RNA, wherein the unwanted library fragments comprise those prepared from unwanted RNA sequences, further comprising: preparing a solid support comprising at least one immobilized oligonucleotide, wherein each immobilized oligonucleotide comprises a nucleic acid sequence corresponding to an unwanted RNA sequence or its complement, adding the library of fragments to the solid support and hybridizing the library fragments to at least one immobilized oligonucleotide to allow binding of unwanted library fragments to at least one immobilized oligonucleotide, and collecting library fragments not bound to at least one immobilized oligonucleotide.
In some embodiments, the at least one immobilized oligonucleotide comprises a sequence comprising any one or more of SEQ ID NOs: 23377-24507 or its complement.
In some embodiments, the depleting unwanted nucleic acid molecules comprises depleting off-target RNA nucleic acid molecules from a nucleic acid sample comprises contacting a nucleic acid sample comprising at least one RNA or DNA target sequence and at least one off-target RNA molecule from a first species with a probe set comprising at least two DNA probes complementary to discontiguous sequences along the full length of the at least one off-target RNA molecule from a second species, thereby hybridizing the DNA probes to the off-target RNA molecules to form DNA: RNA hybrids, wherein each DNA: RNA hybrid is at least 5 bases apart, or at least 10 bases apart, along a given off-target RNA molecule sequence from any other DNA: RNA hybrid, wherein the off-target DNA comprises at least one small noncoding RNA chosen from RN7SK, RN7SL1, RN7SL2, RN7SL5P, RPPH1, SNORD3A; contacting the DNA: RNA hybrids with a ribonuclease that degrades the RNA from the DNA: RNA hybrids, thereby degrading the off-target RNA molecules in the nucleic acid sample to form a degraded mixture; separating the degraded RNA from the degraded mixture; sequencing the remaining RNA from the sample; evaluating the remaining RNA sequences for the presence of off-target RNA molecules from the first species, thereby determining gap sequence regions; and supplementing the probe set with additional DNA probes complementary to discontiguous sequences in one or more of the gap sequence regions.
In some embodiments, the probe set comprises any one or more of SEQ ID NOs: 22917-23376.
In some embodiments, the method further comprises depleting unwanted cDNA library fragments from a library of cDNA fragments prepared from RNA, wherein the unwanted library fragments comprise those prepared from unwanted RNA sequences. In some embodiments, a solid support comprises more than one pool of immobilized oligonucleotides on its surface.
For example, a solid support may comprise a first pool of immobilized oligonucleotides for depleting and a second pool of immobilized oligonucleotides for enriching. In some embodiments, one pool of immobilized oligonucleotides may be blocked (such as with complementary nucleic acid sequences) to avoid binding to complementary library fragments during certain steps of methods using the solid support.
In some embodiments, a solid support has two pools of immobilized oligonucleotides on its surface, wherein the first pool comprises immobilized oligonucleotides each comprising an unwanted RNA sequence and the second pool comprises immobilized oligonucleotides each comprising a solid support adapter sequence that can bind to a library adapter comprised in library fragments. In some embodiments, solid support adapter sequences are bound by adapter complements, wherein the adapter complements can be denatured during a method to allow binding of solid support adapter sequences to library adapters in library fragments. Such a solid support can be used for methods of preparing a depleted library and amplifying the depleted library on the same solid support.
In some embodiments, at least one unwanted RNA sequence has at least 90%, at least 95%, or at least 99% homology to a high-abundance RNA sequence in a sample used to prepare the library of fragments. In some embodiments, all unwanted sequences have at least 90%, at least 95%, or at least 99% homology to a high-abundance RNA sequence in a sample used to prepare the library of fragments.
C. Samples
In some embodiments, the sample comprises a microbe sample, a microbiome sample, a bacteria sample, a yeast sample, a plant sample, an animal sample, a patient sample, an epidemiology sample, an environmental sample, a soil sample, a water sample, a metatranscriptomics sample, or a combination thereof. In some embodiments, samples are from mixed populations of microbes such as microbial populations or viral populations from patients.
In some embodiments the sample is a water sample. In some embodiments, the water sample is a freshwater sample, a wastewater sample, a saline water sample, or a combination thereof. In some embodiments, the sample comprises a wastewater sample.
In some embodiments, the sample may be from a mammal. In some embodiments the sample may be from a human, monkey, bat, dog, cat, horse, goat, sheep, cow, pig, rat and/or mouse. In some instances, reservoirs of coronaviruses or other microbes in animal populations can serve as samples to predict what diseases or strains of diseases may become human pathogens or to compare sequences in animal reservoirs to sequences of pathogens infecting humans.
In some embodiments, samples may be from a patient. In some embodiments, samples may be from a patient with cancer (i.e., an oncology sample). In some embodiments, samples may be from a patient with a rare disease. In some embodiments, samples may be from a patient with coronavirus SARS-COV2 (COVID-19).
In some embodiments, the sample may be a tumor sample. In some embodiments, the sample may be a blood sample, a serum sample, and/or a whole blood sample. In some embodiments the sample may be a tissue sample. In some embodiments the sample may be a fecal sample, a urine sample, a mucus sample, a saliva sample, a lymph sample, a vaginal fluid sample, a semen sample, an amniotic sample, and/or a sweat sample.
D. Library Preparation
Libraries prepared by any method can be used together with the present methods of enriching and/or depleting. In some embodiments, probes are single-stranded to allow for hybridizing and capturing of single-stranded library fragments that are complementary. In some embodiments, specific binding of a single-stranded library fragment to a probe generates a double-stranded oligonucleotide. In some embodiments, the double-stranded oligonucleotide forms a DNA: RNA hybrid. The probe specifically bound to the library fragment may be bound with a high-enough affinity to be recognized for degradation with a ribonuclease. In some embodiments, the off-target RNA molecules are degraded after contacting the sample with a ribonuclease to form a degraded mixture.
As used herein, the term “library” refers to a collection of members. In one embodiment, the library includes a collection of nucleic acid members, for example, a collection of whole genomic, subgenomic fragments, cDNA, cDNA fragments, RNA, RNA fragments, or a combination thereof. In some embodiments, a portion or all library members include a non-target adaptor sequence. The adaptor sequence can be located at one or both ends. The adaptor sequence can be used in, for example, a sequencing method (for example, an NGS method), for amplification, for reverse transcription, or for cloning into a vector.
In some embodiments, this DNA: RNA hybrid-specific cleavage comprises use of RNase H. This methodology is implemented as part of the current Illumina Total RNA Stranded Library Prep workflow and New England Biolabs NEBNext rRNA Depletion Kit and RNA depletion methods as described in U.S. Pat. Nos. 9,745,570 and 9,005,891.
E. Amplification
In some embodiments, methods described herein comprise one or more amplification step. In some embodiments, library fragments are amplified before being added to a solid support. In some embodiments library fragments are amplified after a method of depleting described herein. In some embodiments, amplifying is by PCR amplification.
As used herein, “amplify,” “amplifying,” or “amplification reaction” and their derivatives, refer generally to any action or process whereby at least a portion of a nucleic acid molecule is replicated or copied into at least one additional nucleic acid molecule. The additional nucleic acid molecule optionally includes sequence that is substantially identical or substantially complementary to at least some portion of the template nucleic acid molecule. The template nucleic acid molecule can be single-stranded or double-stranded and the additional nucleic acid molecule can independently be single-stranded or double-stranded. Amplification optionally includes linear or exponential replication of a nucleic acid molecule. In some embodiments, such amplification can be performed using isothermal conditions; in other embodiments, such amplification can include thermocycling. In some embodiments, the amplification is a multiplex amplification that includes the simultaneous amplification of a plurality of target sequences in a single amplification reaction. In some embodiments, “amplification” includes amplification of at least some portion of DNA and RNA based nucleic acids alone, or in combination. The amplification reaction can include any of the amplification processes known to one of ordinary skill in the art. In some embodiments, the amplification reaction includes polymerase chain reaction (PCR).
1. Amplification after Enriching
In some embodiments, collected library fragments are amplified after a method of enriching. In some embodiments, an enriched library is amplified.
In some embodiments, the amplifying is performed with a thermocycler. In some embodiments, the amplifying is by PCR amplification.
As used herein, the term “polymerase chain reaction” (“PCR”) refers to the method as described in U.S. Pat. Nos. 4,683,195 and 4,683,202, which describe a method for increasing the concentration of a segment of a polynucleotide of interest in a mixture of genomic DNA without cloning or purification. This process for amplifying the polynucleotide of interest consists of introducing a large excess of two oligonucleotide primers to the DNA mixture containing the desired polynucleotide of interest, followed by a series of thermal cycling in the presence of a DNA polymerase. The two primers are complementary to their respective strands of the double stranded polynucleotide of interest. The mixture is denatured at a higher temperature first and the primers are then annealed to complementary sequences within the polynucleotide of interest molecule. Following annealing, the primers are extended with a polymerase to form a new pair of complementary strands. The steps of denaturation, primer annealing, and polymerase extension can be repeated many times (referred to as thermocycling) to obtain a high concentration of an amplified segment of the desired polynucleotide of interest. The length of the amplified segment of the desired polynucleotide of interest (amplicon) is determined by the relative positions of the primers with respect to each other, and therefore, this length is a controllable parameter. By virtue of repeating the process, the method is referred to as the “polymerase chain reaction” (hereinafter “PCR”). Because the desired amplified segments of the polynucleotide of interest become the predominant nucleic acid sequences (in terms of concentration) in the mixture, they are said to be “PCR amplified.” In a modification to the method discussed above, the target nucleic acid molecules can be PCR amplified using a plurality of different primer pairs, in some cases, one or more primer pairs per target nucleic acid molecule of interest, thereby forming a multiplex PCR reaction.
In some embodiments, the amplifying is performed without PCR amplification. In some embodiments, the amplifying does not require a thermocycler. In some embodiments, depleting and amplifying after the depleting is performed in a sequencer.
In some embodiments, the amplifying is performed without a thermocycler. In some embodiments, the amplifying is performed by bridge or cluster amplification
F. Sequencing of Enriched Libraries
In some embodiments, a library enriched for enriching for target viral sequences library fragments is sequenced.
In some embodiments, sequencing data generated after enriching for target viral sequences is capable of capturing novel coronaviruses with homology to the sequence in the probe set. In some embodiments, sequencing data generated after enriching for target viral sequences is capable of capturing new or unknown viruses (e.g., new or unknown coronaviruses). In some embodiments, sequencing data generated after enriching for target viral sequences is capable of capturing co-infections. In some embodiments, sequencing data generated after enriching for target viral sequences is capable of capturing specific viral strains (e.g., specific coronavirus strains). In some embodiments, sequencing data generated after enriching for target viral sequences is capable of capturing viral nucleic acids that exhibit resistance. In some embodiments, sequencing data generated after enriching for target viral sequences provides unbiased viral pathogen detection. In some embodiments, sequencing data generated after enriching for target viral sequences is capable of capturing viral nucleic acids present in hospital-associated infection management.
Enriched libraries prepared by the present method can be used with any type of RNA sequencing, such as RNA-seq, small RNA sequencing, long non-coding RNA (lncRNA) sequencing, circular RNA (circRNA) sequencing, targeted RNA sequencing, exosomal RNA sequencing, and degradome sequencing.
Enriched libraries can be sequenced according to any suitable sequencing methodology, such as direct sequencing, including sequencing by synthesis, sequencing by ligation, sequencing by hybridization, nanopore sequencing and the like. In some embodiments, the enriched libraries are sequenced on a solid support. In some embodiments, the solid support for sequencing is the same solid support on which the enriching is performed. In some embodiments, the solid support for sequencing is the same solid support upon which amplification occurs after the enriching.
Flowcells provide a convenient solid support for performing sequencing. One or more library fragments (or amplicons produced from library fragments) in such a format can be subjected to an SBS or other detection technique that involves repeated delivery of reagents in cycles. For example, to initiate a first SBS cycle, one or more labeled nucleotides, DNA polymerase, etc., can be flowed into/through a flowcell that houses one or more amplified nucleic acid molecules. Those sites where primer extension causes a labeled nucleotide to be incorporated can be detected. Optionally, the nucleotides can further include a reversible termination property that terminates further primer extension once a nucleotide has been added to a primer. For example, a nucleotide analog having a reversible terminator moiety can be added to a primer such that subsequent extension cannot occur until a deblocking agent is delivered to remove the moiety. Thus, for embodiments that use reversible termination, a deblocking reagent can be delivered to the flowcell (before or after detection occurs). Washes can be carried out between the various delivery steps. The cycle can then be repeated n times to extend the primer by n nucleotides, thereby detecting a sequence of length n. Exemplary SBS procedures, fluidic systems and detection platforms that can be readily adapted for use with amplicons produced by the methods of the present disclosure are described, for example, in Bentley et al., Nature 456:53-59 (2008), WO 04/018497; U.S. Pat. No. 7,057,026; WO 91/06678; WO 07/123744; U.S. Pat. Nos. 7,329,492; 7,211,414; 7,315,019; 7,405,281, and US 2008/0108082, each of which is incorporated herein by reference.
The term “flow cell” as used herein refers to a chamber comprising a solid surface across which one or more fluid reagents can be flowed. Examples of flow cells and related fluidic systems and detection platforms that can be readily used in the methods of the present disclosure are described, for example, in Bentley et al., Nature 456:53-59 (2008); WO 04/018497; WO 91/06678; WO 07/123744; U.S. Pat. Nos. 7,057,026; 7,211,414; 7,315,019; 7,329,492; 7,405,281; and US Pat. Publication No. 2008/0108082.
G. Whole Genome Sequencing, Amplicon Sequencing, Metagenomic Analysis, and Metatranscriptomic Analysis
In some embodiments, samples are sequenced using whole-genome sequencing and/or amplicon sequencing. Whole genome sequencing refers to sequencing the genome of any organism including viral pathogens (e.g., coronaviruses) and host organisms. For example, whole genome sequencing may be performed on a microbial isolate. Transmission dynamics may be evaluated by whole genome sequencing. Whole genome sequencing also provides useful information on strain characterization, resistance detection, and hospital-associated infection management.
In some embodiments, samples are sequenced using amplicon sequencing. The term “amplicon” refers to the resultant mixture of compounds after two or more cycles of the PCR steps of denaturation, annealing and extension. Thus, amplicon sequencing is the sequencing of amplicons and this can provide useful information on variant identification and characterization. In some embodiments, amplicon sequencing encompasses amplification of one or more segments of one or more target sequences, which can be performed by using probes to target and amplify regions of interest, followed by sequencing, such as next-generation sequencing. Amplicon sequencing may be performed on a variety of samples, including patient samples or microbial isolates, and is useful for strain characterization. It is also useful for viral resequencing and resistance detection.
In some embodiments, additional information may be obtained about samples using metagenomic and/or metatranscriptomic analyses. Metagenomic and/or metatranscriptomic analysis may be performed on patient samples and may provide unbiased viral pathogen detection. In some embodiments, metagenomic or metatranscriptomic analyses comprises sequencing the genomes of a plurality of individuals of different species in a given sample. In some embodiments, metagenomic or metatranscriptomic analyses is done without prior knowledge regarding the biological species in the sample, whether they be viral or human. In some embodiments, metagenomic or metatranscriptomic analyses enables determination of which species are present, and their relative abundances. Thus, metagenomic and/or metatranscriptomic analysis may be useful for unknown viral pathogen detection, co-infection detection, resistance detection, and/or strain characterization.
In some embodiments, whole genome sequencing, amplicon sequencing, metgenomic analysis, and/or metatranscriptomic analyses may be used in combination with each other.
IV. Kits
Described herein is a kit comprising any of the compositions described herein in Section II, Compositions, above.
Disclosed herein are also kits for depleting or enriching libraries. In some embodiments, the kit comprises a solid support disclosed herein and instructions for using the solid support. Such a kit may further comprise reagents for preparing a cDNA library from RNA, such as reagents for a stranded method of cDNA preparation from a sample comprising RNA, as described below.
In some embodiments the kit comprises at least one DNA probe comprising at least one sequence comprising at least one of SEQ ID NOs: 1-2909 and a buffer.
In some embodiments, the buffer is a wash buffer and/or an elution buffer.
In some embodiments, the kit further comprises an RNA depletion buffer, a probe depletion buffer, and/or a probe removal buffer.
In some embodiments, the kit further comprises a ribonuclease; a DNase; and RNA purification beads. In some embodiments, the ribonuclease is RNase H.
In some embodiments, the kit comprises a buffer and nucleic acid purification medium. In some embodiments, the buffer is an RNA depletion buffer, a probe depletion buffer, and/or a probe removal buffer.
In some embodiments, the kit comprises a nucleic acid destabilizing chemical. In some embodiments, the nucleic acid destabilizing chemical comprises betaine, DMSO, formamide, glycerol, or a derivative thereof, or a mixture thereof. In some embodiments, the nucleic acid destabilizing chemical comprises formamide.
Throughout this application and claims, the term “and/or” means one or more of the listed elements or a combination of any two or more of the listed elements.
The term “comprises” and variations thereof do not have a limiting meaning where these terms appear in the description and claims.
It is understood that wherever embodiments are described herein with the language “include,” “includes,” or “including,” and the like, otherwise analogous embodiments described in terms of “consisting of” and/or “consisting essentially of” are also provided. The term “consisting of” is limited to whatever follows the phrase “consisting of.” That is, “consisting of” indicates that the listed elements are required or mandatory, and that no other elements may be present. The term “consisting essentially of” indicates that any elements listed after the phrase are included, and that other elements than those listed may be included provided that those elements do not interfere with or contribute to the activity or action specified in the disclosure for the listed elements.
Unless otherwise specified, “a,” “an,” “the,” and “at least one” are used interchangeably and mean one or more than one.
As used herein, the term “each,” when used in reference to a collection of items, is intended to identify an individual term in the collection but does not necessarily refer to every term in the collection unless the context clearly dictates otherwise.
The recitations of numerical ranges by endpoints include all numbers subsumed within that range (e.g., 1 to 5 includes 1, 1.5, 2, 2.75, 3, 3.80, 4, 5, etc.).
For any method disclosed herein that includes discrete steps, the steps may be conducted in any feasible order. And, as appropriate, any combination of two or more steps may be conducted simultaneously.
The above summary of the present invention is not intended to describe each disclosed embodiment or every implementation of the present invention. The description that follows more particularly exemplifies illustrative embodiments. In several places throughout the application, guidance is provided through lists of examples, which examples can be used in various combinations. In each instance, the recited list serves only as a representative group and should not be interpreted as an exclusive list.
Reference throughout this specification to “one embodiment,” “an embodiment,” “certain embodiments,” or “some embodiments,” etc., means that a particular feature, configuration, composition, or characteristic described in connection with the embodiment is included in at least one embodiment of the disclosure. Thus, the appearances of such phrases in various places throughout this specification are not necessarily referring to the same embodiment of the disclosure. Furthermore, the particular features, configurations, compositions, or characteristics may be combined in any suitable manner in one or more embodiments.
Unless otherwise indicated, all numbers expressing quantities of components, molecular weights, and so forth used in the specification and claims are to be understood as being modified in all instances by the term “about.” Accordingly, unless otherwise indicated to the contrary, the numerical parameters set forth in the specification and claims are approximations that may vary depending upon the desired properties sought to be obtained by the present invention. At the very least, and not as an attempt to limit the doctrine of equivalents to the scope of the claims, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.
All headings are for the convenience of the reader and should not be used to limit the meaning of the text that follows the heading, unless so specified.
Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. All numerical values, however, inherently contain a range necessarily resulting from the standard deviation found in their respective testing measurements.
EXAMPLES
The following examples are illustrative only and are not intended to limit the scope of the application. Modifications will be apparent and understood by skilled artisans and are included within the spirit and under the disclosure of this application.
Example 1. Preparation of Probes to Improve Enrichment of Coronaviruses of Interest in Wastewater Samples
A. Probe Design
Probes were designed by a proprietary algorithm for enrichment probes running on a Linux server (first iteration probes). The weighting for spacing and probe scoring variables were set to 6 and 1 respectively. Probe spacing was set to ‘adjacent’, or 80 bp center to center.
The first iteration probes aimed to strike a balance between capturing the most important virus species/the ones most heavily represented as isolates in NCBI nucleotide database and capturing a diverse set of sequences from the four coronavirus genera. Choice of diverse sequences was based on phylogenetic trees generated by sequence alignments using the MAFFT alignment algorithm set to the FFT-NS-i iterative refinement method and generating trees using Jalview average distance method. See Table 1.
TABLE 1
|
|
FASTA_ID
Name
Accession
Genus
Subgenus
|
|
229E
Human coronavirus 229E
Consensus
Alphacoronavirus
Duvinacovirus
|
AlphaCoV1
Phylogenetic grouping of various animal
Consensus
Alphacoronavirus
Tegacovirus
|
transmissible gastroenteritis viruses
|
AlphaSp
Phylogenetic grouping of various
Consensus
Alphacoronavirus
unclassified
|
alphacoronavirus sp. strains
|
Anlong
Bat coronavirus isolate Anlong
Consensus
unclassified
n/a
|
Coronavirinae
|
AvianCoV
Phylogenetic grouping of avian
Consensus
Gammacoronavirus
Igacovirus
|
coronavirus (infectious bronchitis) strains
|
Beluga
Beluga Whale coronavirus
Consensus
Gammacoronavirus
Cegacovirus
|
BetaCoV1
Phylogenetic grouping of various
Consensus
Betacoronavirus
Embecovirus
|
embecovirus isolates
|
BetaSp
Phylogenetic grouping of rodent
Consensus
Betacoronavirus
Embecovirus
|
embecovirus isolates
|
CDPHE15
Bat coronavirus CDPHE15
Consensus
Alphacoronavirus
Colacovirus
|
Delta
Consensus sequences of NCBI
Consensus
Deltacoronavirus
multiple
|
Deltacoronavirus sequences
|
HKU1
Human coronavirus HKU1
Consensus
Betacoronavirus
Embecovirus
|
HKU2
Rhinolophus bat coronavirus HKU2
Consensus
Alphacoronavirus
Rhinacovirus
|
HKU4
Tylonycteris bat coronavirus HKU4
Consensus
Betacoronavirus
Merbecovirus
|
HKU5
Pipistrellus bat coronavirus HKU5
Consensus
Betacoronavirus
Merbecovirus
|
HKU8
Miniopterus bat coronavirus HKU8
Consensus
Alphacoronavirus
Minunacovirus
|
HKU9
Rousettus bat coronavirus HKU9
Consensus
Betacoronavirus
Nobecovirus
|
HKU10
Bat coronavirus HKU10
Consensus
Alphacoronavirus
Decacovirus
|
HKU14
rabbit coronavirus HKU14
Consensus
Betacoronavirus
Embecovirus
|
HKU15
Porcine coronavirus HKU15
Consensus
Deltacoronavirus
Buldecovirus
|
HKU25
Hypsugo bat coronavirus HKU25
Consensus
Betacoronavirus
Merbecovirus
|
HKU31
Erinaceus hedgehog coronavirus HKU31
Consensus
Betacoronavirus
Merbecovirus
|
MERS
Middle East respiratory syndrome
Consensus
Betacoronavirus
Merbecovirus
|
coronavirus
|
MurineCoV
Phylogenetic grouping of rodent
Consensus
Betacoronavirus
Embecovirus
|
embecovirus isolates
|
NL63
Human Coronavirus NL63
Consensus
Alphacoronavirus
Setracovirus
|
PEDV
Porcine epidemic diarrhea virus
Consensus
Alphacoronavirus
Pedacovirus
|
SARSCoV2
Severe acute respiratory syndrome
Consensus
Betacoronavirus
Sarbecovirus
|
coronavirus 2
|
SARSCoV
Severe acute respiratory syndrome
Consensus
Betacoronavirus
Sarbecovirus
|
coronavirus
|
Sparrow
Sparrow deltacoronavirus
Consensus
Deltacoronavirus
Buldecovirus
|
|
A further round of probe design using the same methods was targeted to a larger and yet more diverse set of coronavirus isolates (second iteration probes). See Table 2. In addition to the publicly available sequences provided in Table 2, second iteration probes were also designed to a number of proprietary sequences.
TABLE 2
|
|
FASTA_ID
Name
Accession
Genus
Subgenus
|
|
229ER
Rousettus aegyptiacus
MN611517
Alphacoronavirus
Duvinacovirus
|
bat coronavirus
|
229E_related
|
AcCoV_JC34
Coronavirus
KX964649
Alphacoronavirus
Luchacovirus
|
AcCoV_JC34
|
Alpha2
Mink coronavirus strain
HM245926
Alphacoronavirus
Minacovirus
|
WD1133
|
Alpha3398
Alphacoronavirus
HM245925
Alphacoronavirus
Minacovirus
|
Bat_CoV/P.kuhlii/Italy/
|
3398_19/2015
|
AlphaBat
Bat alphacoronavirus
MN065811
Alphacoronavirus
unclassified
|
AlphaMink
Alphacoronavirus
MF113046
Alphacoronavirus
Minacovirus
|
Mink/China/1/2016
|
BatCoV
Bat coronavirus
MG916904
unclassified
n/a
|
BtCoV/Rh/YN2012
Coronavirinae
|
isolate
|
BtCoV/Rh/YN2012_Ra13591
|
BatCoV1A
Bat coronavirus 1A
EU420137
Alphacoronavirus
Minunacovirus
|
BM48_31
Bat coronavirus
GU190215
Betacoronavirus
Sarbecovirus
|
BM48_31/BGR/2008
|
BtKYNL63
NL63_related bat
KY073746
Alphacoronavirus
Setracovirus
|
coronavirus strain
|
BtKYNL63_9b
|
BtRf_AlphaCoV
BtRf_AlphaCoV/YN2012
KJ473808
Alphacoronavirus
Rhinacovirus
|
BtRl_BetaCoV
Coronavirus
MK211374
Betacoronavirus
Sarbecovirus
|
BtRl_BetaCoV/SC2018
|
BtSk_AlphaCoV
Coronavirus
MK211372
Alphacoronavirus
Pedacovirus
|
BtSk_AlphaCoV/GX2018D
|
CHB25
Hipposideros pomona
MN611525
Alphacoronavirus
Decacovirus
|
bat coronavirus CHB25
|
FelineAlpha1
Feline alphacoronavirus 1
MH817484
Alphacoronavirus
Tegacovirus
|
FerretCoV
Ferret coronavirus
NC_030292
Alphacoronavirus
Minacovirus
|
GCCDC1
Rousettus bat
KU762338
Betacoronavirus
Nobecovirus
|
coronavirus GCCDC1
|
HghCoV1
Hedgehog coronavirus 1
NC_039207
Betacoronavirus
Merbecovirus
|
HKU10R
Hipposideros pomona
MN611523
Alphacoronavirus
Decacovirus
|
bat coronavirus
|
HKU10_related
|
HKU11
Bulbul coronavirus
FJ376620
Deltacoronavirus
Buldecovirus
|
HKU11
|
HKU12
Thrush coronavirus
FJ376621
Deltacoronavirus
Buldecovirus
|
HKU12
|
HKU16
White_eye coronavirus
JQ065044
Deltacoronavirus
Buldecovirus
|
HKU16
|
HKU17
Sparrow coronavirus
JQ065045
Deltacoronavirus
Buldecovirus
|
HKU17
|
HKU18
Magpie_robin
JQ065046
Deltacoronavirus
Buldecovirus
|
coronavirus HKU18
|
HKU19
Night heron coronavirus
JQ065047
Deltacoronavirus
Herdecovirus
|
HKU19
|
HKU20_FJ376622
Munia coronavirus
FJ376622
Deltacoronavirus
Buldecovirus
|
HKU20
|
HKU20_JQ065048
Wigeon coronavirus
JQ065048
Deltacoronavirus
Andecovirus
|
HKU20
|
HKU21
Common moorhen
JQ065049
Deltacoronavirus
Moordecovirus
|
coronavirus HKU21
|
HKU23
Camel coronavirus
KT368891
Betacoronavirus
Embecovirus
|
HKU23
|
HKU24
China Rattus coronavirus
NC_026011
Betacoronavirus
Embecovirus
|
HKU24
|
HKU2R
Rhinolophus affinis bat
MN611522
Alphacoronavirus
Rhinacovirus
|
coronavirus
|
HKU2_related
|
HKU32
Rhinolophus bat
MK720945
Alphacoronavirus
Decacovirus
|
coronavirus HKU32
|
HKU33
Tylonycteris bat
MK720944
Alphacoronavirus
Nyctacovirus
|
coronavirus HKU33
|
HKU4R
Tylonycteris pachypus
MN611519
Betacoronavirus
Merbecovirus
|
bat coronavirus
|
HKU4_related
|
HKU5R
Pipistrellus abramus bat
MN611520
Betacoronavirus
Merbecovirus
|
coronavirus
|
HKU5_related
|
HKU8R
Miniopterus pusillus bat
MN611518
Alphacoronavirus
Minunacovirus
|
coronavirus
|
HKU8_related
|
Hp_Beta
Bat Hp_betacoronavirus
KF636752
Betacoronavirus
Hibecovirus
|
Zhejiang2013
|
HuB
Rhinolophus
KJ473810
Alphacoronavirus
Decacovirus
|
ferrumequinum
|
alphacoronavirus
|
HuB_2013
|
MbCoV1
Miniopterus bat
EU420138
Alphacoronavirus
Minunacovirus
|
coronavirus 1
|
MinkCoV1
Mink coronavirus 1
HM245925
Alphacoronavirus
Minacovirus
|
MrAlphaSax
Myotis ricketti
KJ473806
Alphacoronavirus
Myotacovirus
|
alphacoronavirus
|
Sax_2011
|
MsbCoV1
Miniopterus schreibersii
MN611524
Alphacoronavirus
Minunacovirus
|
bat coronavirus 1_related
|
MvAlphaSC
Nyctalus velutinus
KJ473809
Alphacoronavirus
Nyctacovirus
|
alphacoronavirus
|
SC_2013
|
Quail
Quail deltacoronavirus
MH532440
Deltacoronavirus
Buldecovirus
|
SARSRC
Severe acute respiratory
DQ648856
Betacoronavirus
Sarbecovirus
|
syndrome_related
|
coronavirus
|
Sb512
Scotophilus bat
NC_009657
Alphacoronavirus
Pedacovirus
|
coronavirus 512
|
SEAC
Swine enteric
MK977618
Alphacoronavirus
Rhinacovirus
|
alphacoronavirus
|
Sk512R
Scotophilus kuhlii bat
MN611521
Alphacoronavirus
Pedacovirus
|
coronavirus 512_related
|
WIV16
SARS_like coronavirus
KT444582
Betacoronavirus
Sarbecovirus
|
WIV16
|
BtCoV_008_16
Bat alphacoronavirus
MN065811
Alphacoronavirus
unclassified
|
strain
|
BtCoV/008_16/M.bra/FIN/
|
2016
|
CanadaGoose
Canada goose
MK359255
Gammacoronavirus
unclassified
|
coronavirus strain
|
Cambridge_Bay_2017
|
Lucheng19
Lucheng Rn rat
KF294380
Alphacoronavirus
Luchacovirus
|
coronavirus isolate
|
Lucheng-19
|
Shrew
Wencheng Sm shrew
NC_035191
Alphacoronavirus
unclassified
|
coronavirus isolate
|
Xingguo-101
|
M95169
Avian-infectious-
M95169
Gammacoronavirus
Igacovirus
|
bronchitis-virus-pol-
|
protein,-spike-protein,-
|
small-virion-associated-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
AF029248
Mouse-hepatitis-virus-
AF029248
Betacoronavirus
Embecovirus
|
strain-MHV-A59-C12-
|
mutant
|
AF201929
Murine-hepatitis-virus-
AF201929
Betacoronavirus
Embecovirus
|
strain-2
|
AF208066
Murine-hepatitis-virus-
AF208066
Betacoronavirus
Embecovirus
|
strain-Penn-97-1
|
AF208067
Murine-hepatitis-virus-
AF208067
Betacoronavirus
Embecovirus
|
strain-ML-10
|
AJ271965
Transmissible-
AJ271965
Alphacoronavirus
Tegacovirus
|
gastroenteritis-virus-
|
complete-genome,-
|
genomic-RNA
|
AJ311317
Avian-infectious-
AJ311317
Gammacoronavirus
Igacovirus
|
bronchitis-virus-(strain-
|
Beaudette-CK)-
|
complete-genomic-RNA
|
AY338732
Avian-infectious-
AY338732
Gammacoronavirus
Igacovirus
|
bronchitis-virus-1a-
|
protein-(1a),-1ab-
|
polyprotein-(1),-S-
|
protein-(S),-3a-protein-
|
(3a),-3b-protein-(3b),-E-
|
protein-(3c),-M-protein-
|
(M),-5a-protein-(5a),-5b-
|
protein-(5b),-and-
|
nucleocapsid-protein-
|
(N)-genes
|
AY692454
Avian-infectious-
AY692454
Gammacoronavirus
Igacovirus
|
bronchitis-virus-
|
polyprotein-1a,-
|
polyprotein-1b,-spike-
|
protein,-3a-protein,-3b-
|
protein,-small-envelope-
|
protein,-membrane-
|
protein,-5a-protein,-5b-
|
protein,-and-
|
nucleocapsid-protein-
|
mRNAs
|
AY994055
Feline-infectious-
AY994055
Alphacoronavirus
Tegacovirus
|
peritonitis-virus
|
AY597011
Human-coronavirus-
AY597011
Betacoronavirus
Embecovirus
|
HKU1-genotype-A
|
NC_009021
Rousettus-bat-
NC_009021
Betacoronavirus
Nobecovirus
|
coronavirus-HKU9
|
NC_010438
Bat-coronavirus-HKU8
NC_010438
Alphacoronavirus
Minunacovirus
|
NC_010646
Beluga-Whale-
NC_010646
Gammacoronavirus
Cegacovirus
|
coronavirus-SW1
|
NC_010800
Turkey-coronavirus
NC_010800
Gammacoronavirus
Igacovirus
|
NC_012936
Rat-coronavirus-Parker
NC_012936
Betacoronavirus
Embecovirus
|
FN430414
Infectious-bronchitis-
FN430414
Gammacoronavirus
Igacovirus
|
virus-ITA/90254/2005
|
FN430415
Infectious-bronchitis-
FN430415
Gammacoronavirus
Igacovirus
|
virus-NGA/A116E7/2006
|
AB551247
Murine-hepatitis-virus-
AB551247
Betacoronavirus
Embecovirus
|
RNA,-strain:-MHV-MI
|
HQ166910
Zaria-bat-coronavirus-
HQ166910
Betacoronavirus
Hibecovirus
|
strain-ZBCoV
|
GU002364
Fathead-minnow-
GU002364
Bafinivirus
Pimfabavirus
|
nidovirus-replicase-
|
polyprotein-1ab-
|
(pp1ab),-replicase-
|
polyprotein-1a-(pp1a),-
|
spike-glycoprotein-(S),-
|
membrane-protein-(M),-
|
and-nucleocapsid-
|
protein-(N)-genes
|
NC_017083
Rabbit-coronavirus-
NC_017083
Betacoronavirus
Embecovirus
|
HKU14
|
NC_018871
Rousettus-bat-
NC_018871
Alphacoronavirus
Decacovirus
|
coronavirus-HKU10
|
NC_022103
Bat-coronavirus-
NC_022103
Alphacoronavirus
Colacovirus
|
CDPHE15/USA/2006
|
KF850449
Rat-coronavirus-strain-
KF850449
Betacoronavirus
Embecovirus
|
mouse_lab/USA/
|
MG6644_HOLMES10/1996
|
KF294370
Longquan-R1-rat-
KF294370
Betacoronavirus
Embecovirus
|
coronavirus-isolate-
|
Longquan-189-orf1ab-
|
polyprotein-gene;-and-
|
hemagglutinin-esterase,-
|
spike-protein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KF294371
Longquan-R1-rat-
KF294371
Betacoronavirus
Embecovirus
|
coronavirus-isolate-
|
Longquan-370-orf1ab-
|
polyprotein-gene;-and-
|
hemagglutinin-esterase,-
|
spike-protein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KF294372
Longquan-R1-rat-
KF294372
Betacoronavirus
Embecovirus
|
coronavirus-isolate-
|
Longquan-708-orf1ab-
|
polyprotein-gene;-and-
|
hemagglutinin-esterase,-
|
spike-protein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
LN610099
Guinea-fowl-coronavirus-
LN610099
Gammacoronavirus
Igacovirus
|
GfCoV/FR/2011-
|
complete-genome
|
LC061272
Equine-coronavirus-
LC061272
Betacoronavirus
Embecovirus
|
RNA,-strain:-Tokachi09
|
LC061273
Equine-coronavirus-
LC061273
Betacoronavirus
Embecovirus
|
RNA,-strain:-Obihiro 12-1
|
LC061274
Equine-coronavirus-
LC061274
Betacoronavirus
Embecovirus
|
RNA,-strain:-Obihiro12-2
|
KT253269
229E-related-bat-
KT253269
Alphacoronavirus
Duvinacovirus
|
coronavirus-isolate-
|
BtCoV/KW2E-
|
F151/Hip_cf._rub/GHA/
|
2011-orf1ab,-orf1a,-
|
spike-glycoprotein,-
|
orf4,-E,-M,-N,-and-orf8-
|
genes
|
KT253270
229E-related-bat-
KT253270
Alphacoronavirus
Duvinacovirus
|
coronavirus-isolate-
|
BtCoV/FO1A-
|
F2/Hip_aba/GHA/2010-
|
orf1ab,-orf1a,-spike-
|
glycoprotein,-orf4,-E,-
|
M,-N,-and-orf8-genes
|
KT253271
229E-related-bat-
KT253271
Alphacoronavirus
Duvinacovirus
|
coronavirus-isolate-
|
BtCoV/KW2E-
|
F56/Hip_cf._rub/GHA/2
|
011-orf1ab,-orf1a,-spike-
|
glycoprotein,-orf4,-E,-
|
M,-N,-and-orf8-genes
|
KT253272
229E-related-bat-
KT253272
Alphacoronavirus
Duvinacovirus
|
coronavirus-isolate-
|
BtCoV/AT1A-
|
F1/Hip_aba/GHA/2010-
|
orf1ab,-orf1a,-spike-
|
glycoprotein,-orf4,-E,-
|
M,-N,-and-orf8-genes
|
NC_028752
Camel-alphacoronavirus-
NC_028752
Alphacoronavirus
Duvinacovirus
|
isolate-
|
camel/Riyadh/Ry141/2015
|
NC_028806
Swine-enteric-
NC_028806
Alphacoronavirus
Tegacovirus
|
coronavirus-strain-
|
Italy/213306/2009
|
LC119077
Ferret-coronavirus-
LC119077
Alphacoronavirus
Minacovirus
|
genomic-RNA,-strain:-
|
FRCoV4370
|
NC_032107
NL63-related-bat-
NC_032107
Alphacoronavirus
Setracovirus
|
coronavirus-strain-
|
BtKYNL63-9a
|
KU556804
Infectious-bronchitis-
KU556804
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
Ck/Aus/N1/88
|
KU556805
Infectious-bronchitis-
KU556805
Gammacoronavirus
Igacovirus
|
virus-isolate-Armidale-A3
|
KU556806
Infectious-bronchitis-
KU556806
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
Ck/Aus/N1/03
|
KU556807
Infectious-bronchitis-
KU556807
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
Ck/Aus/N1/08
|
NC_032730
Lucheng-Rn-rat-
NC_032730
Alphacoronavirus
Luchacovirus
|
coronavirus-isolate-
|
Lucheng-19
|
KX721498
Infectious-bronchitis-
KX721498
Gammacoronavirus
Igacovirus
|
virus-isolate-SCZJ-2
|
KY419111
Porcine-
KY419111
Betacoronavirus
Embecovirus
|
hemagglutinating-
|
encephalomyelitis-virus-
|
strain-PHEV-
|
CoV/swine/USA/15TOSU1727/
|
2015
|
KY588134
Infectious-bronchitis-
KY588134
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
Pakistan/Mass/1003/2A/2015
|
KY588135
Infectious-bronchitis-
KY588135
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
Pakistan/Mass/1009/13A/2015
|
NC_034440
Bat-coronavirus-isolate-
NC_034440
unclassified
n/a
|
PREDICT/PDF-2180
Coronavirinae
|
KY983584
Human-coronavirus-
KY983584
Betacoronavirus
Embecovirus
|
HKU1-strain-SC2628
|
NC_034976
Goat-torovirus-strain-SZ
NC_034976
Gammacoronavirus
Igacovirus
|
KY273667
Infectious-bronchitis-
KY273667
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
Jordan/Mass/15/2004
|
LC215871
Ferret-coronavirus-
LC215871
Alphacoronavirus
Minacovirus
|
genomic-RNA,-strain:-
|
ferret063
|
KX722531
Feline-coronavirus-
KX722531
Alphacoronavirus
Tegacovirus
|
isolate-Cat-2-Holstebro
|
KY370043
Rodent-coronavirus-
KY370043
unclassified
n/a
|
isolate-RtRn-
Coronavirinae
|
CoV/YN2013-ORF1ab-
|
polyprotein,-
|
hemagglutinin-esterase-
|
protein,-spike-
|
glycoprotein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370044
Rodent-coronavirus-
KY370044
unclassified
n/a
|
isolate-RtAs-
Coronavirinae
|
CoV/IM2014-ORF1ab-
|
polyprotein,-
|
hemagglutinin-esterase-
|
protein,-spike-
|
glycoprotein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370045
Rodent-coronavirus-
KY370045
unclassified
n/a
|
isolate-RtMruf-CoV-
Coronavirinae
|
1/JL2014-ORF1ab-
|
polyprotein,-spike-
|
glycoprotein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370046
Rodent-coronavirus-
KY370046
unclassified
n/a
|
isolate-RtMruf-CoV-
Coronavirinae
|
2/JL2014-ORF1ab-
|
polyprotein,-
|
hemagglutinin-esterase-
|
protein,-spike-
|
glycoprotein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370048
Rodent-coronavirus-
KY370048
unclassified
n/a
|
isolate-RtMm-
Coronavirinae
|
CoV/GD2015-ORF1ab-
|
polyprotein,-
|
hemagglutinin-esterase-
|
protein,-spike-
|
glycoprotein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370049
Rodent-coronavirus-
KY370049
unclassified
n/a
|
isolate-RtNn-
Coronavirinae
|
CoV/SAX2015-ORF1ab-
|
polyprotein,-
|
hemagglutinin-esterase-
|
protein,-spike-
|
glycoprotein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370050
Rodent-coronavirus-
KY370050
unclassified
n/a
|
isolate-RtRl-
Coronavirinae
|
CoV/FJ2015-ORF1ab-
|
polyprotein,-spike-
|
glycoprotein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370051
Rodent-coronavirus-
KY370051
unclassified
n/a
|
isolate-RtBi-
Coronavirinae
|
CoV/FJ2015-ORF1ab-
|
polyprotein,-
|
hemagglutinin-esterase-
|
protein,-spike-
|
glycoprotein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370052
Rodent-coronavirus-
KY370052
unclassified
n/a
|
isolate-RtMm-CoV-
Coronavirinae
|
1/IM2014-ORF1ab-
|
polyprotein,-
|
hemagglutinin-esterase-
|
protein,-spike-
|
glycoprotein,-envelope-
|
protein,-membrane-
|
protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370053
Shrew-coronavirus-
KY370053
Alphacoronavirus
Soracovirus
|
isolate-Shrew-
|
CoV/Tibet2014-
|
ORF1ab-polyprotein,-
|
spike-glycoprotein,-
|
envelope-protein,-
|
membrane-protein,-and-
|
nucleocapsid-protein-
|
genes
|
KY370054
Rodent-coronavirus-
KY370054
unclassified
n/a
|
isolate-RtClan-
Coronavirinae
|
CoV/GZ2015-ORF1ab-
|
polyprotein-gene;-and-
|
spike-glycoprotein,-
|
envelope-protein,-
|
membrane-protein,-and-
|
nucleocapsid-protein-
|
genes
|
MF416379
Murine-hepatitis-virus-
MF416379
Betacoronavirus
Embecovirus
|
isolate-
|
MHV/NYC/Manhattan/poolF5
|
MG428700
Human-coronavirus-
MG428700
Alphacoronavirus
Setracovirus
|
NL63-isolate-
|
Kilifi_HH_1602_01-Jun-2010
|
MG693168
Bat-coronavirus-isolate-
MG693168
unclassified
n/a
|
CMR704-P12-ORF1a,-
Coronavirinae
|
ORF1b,-spike-protein,-
|
ORF3,-Membrane-
|
protein,-capsid,-
|
hypothetical-protein-
|
ORFx,-and-hypothetical-
|
protein-ORFy-genes
|
MG693169
Bat-coronavirus-isolate-
MG693169
unclassified
n/a
|
CMR900-ORF1a,-
Coronavirinae
|
ORF1b,-Spike-protein,-
|
ORF3,-Membrane-
|
protein,-capsid,-
|
hypothetical-protein-
|
ORFx,-and-hypothetical-
|
protein-ORFy-genes
|
MG693170
Bat-coronavirus-isolate-
MG693170
unclassified
n/a
|
CMR66-ORF1a,-
Coronavirinae
|
ORF1b,-Spike-protein,-
|
ORF3,-Membrane-
|
protein,-capsid,-
|
hypothetical-protein-
|
ORFx,-and-hypothetical-
|
protein-ORFy-genes
|
MG693171
Bat-coronavirus-isolate-
MG693171
unclassified
n/a
|
CMR891-892-ORF1a-
Coronavirinae
|
gene;-and-ORF1b,-
|
Spike-protein,-ORF3,-
|
Membrane-protein,-
|
capsid,-hypothetical-
|
protein-ORFx,-and-
|
hypothetical-protein-
|
ORFy-genes
|
MG693172
Bat-coronavirus-isolate-
MG693172
unclassified
n/a
|
CMR705-P13-ORF1a,-
Coronavirinae
|
ORF1b,-Spike-protein,-
|
ORF3,-Membrane-
|
protein,-capsid,-
|
hypothetical-protein-
|
ORFx,-and-hypothetical-
|
protein-ORFy-genes
|
NC_038294
Betacoronavirus-
NC_038294
Betacoronavirus
Merbecovirus
|
England-1-isolate-
|
H123990006
|
MK163627
Alphacoronavirus-
MK163627
Alphacoronavirus
|
UKRn3-pp1a,-pp1ab,-
|
NS2,-spike,-NS4,-E-
|
protein,-M-protein,-
|
NS7a,-nucleocapsid,-
|
and-NS9-genes
|
MG923574
Bat-alphacoronavirus-
MG923574
Alphacoronavirus
unclassified
|
isolate-
|
BtCoV/020_16/M.dau/FIN/
|
2016-polyprotein,-
|
spike-protein,-
|
hypothetical-protein,-
|
envelope-protein,-
|
membrane-protein,-and-
|
nucleocapsid-protein-
|
genes
|
MK953937
Infectious-bronchitis-
MK953937
Gammacoronavirus
Igacovirus
|
virus-strain-Brazil/SP55
|
MK957244
Infectious-bronchitis-
MK957244
Gammacoronavirus
Igacovirus
|
virus-strain-PR05
|
MK957245
Infectious-bronchitis-
MK957245
Gammacoronavirus
Igacovirus
|
virus-strain-PR01
|
MK204393
Avian-coronavirus-non-
MK204393
Gammacoronavirus
Igacovirus
|
structural-polyprotein-
|
(ORF1ab)-and-spike-
|
protein-(S)-genes
|
MK204411
Avian-coronavirus-non-
MK204411
Gammacoronavirus
Igacovirus
|
structural-polyprotein-
|
(ORF1ab),-spike-
|
protein-(S),-3a-(3a),-3b-
|
(3b),-envelope-protein-
|
(3c),-membrane-
|
glycoprotein,-5a-(5a),-
|
5b-(5b),-and-
|
nucleocapsid-(N)-genes
|
LC469308
Bat-coronavirus-Vs-
LC469308
unclassified
n/a
|
CoV-1-genomic-RNA
Coronavirinae
|
MK618759
Infectious-bronchitis-
MK618759
Gammacoronavirus
Igacovirus
|
virus-isolate-K047-12
|
MT040333
Pangolin-coronavirus-
MT040333
Betacoronavirus
Sarbecovirus
|
isolate-PCoV_GX-P4L
|
MT040334
Pangolin-coronavirus-
MT040334
Betacoronavirus
Sarbecovirus
|
isolate-PCoV_GX-P1E
|
MT040335
Pangolin-coronavirus-
MT040335
Betacoronavirus
Sarbecovirus
|
isolate-PCoV_GX-P5L
|
MT040336
Pangolin-coronavirus-
MT040336
Betacoronavirus
Sarbecovirus
|
isolate-PCoV_GX-P5E
|
MT084071
Pangolin-coronavirus-
MT084071
Betacoronavirus
Sarbecovirus
|
isolate-MP789-genomic-
|
sequence
|
MT121216
Pangolin-coronavirus-
MT121216
Betacoronavirus
Sarbecovirus
|
isolate-MP789
|
MN690608
Bottlenose-dolphin-
MN690608
Gammacoronavirus
Cegacovirus
|
coronavirus-strain-
|
37112-1
|
MN690609
Bottlenose-dolphin-
MN690609
Gammacoronavirus
Cegacovirus
|
coronavirus-strain-
|
37112-2
|
MN690610
Bottlenose-dolphin-
MN690610
Gammacoronavirus
Cegacovirus
|
coronavirus-strain-
|
37112-3
|
MN690611
Bottlenose-dolphin-
MN690611
Gammacoronavirus
Cegacovirus
|
coronavirus-strain-
|
37112-4
|
MN262644
Avian-coronavirus-
MN262644
Gammacoronavirus
Igacovirus
|
strain-CV10
|
MN711790
Infectious-bronchitis-
MN711790
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
GA/1472/2004
|
MN199462
Infectious-bronchitis-
MN199462
Gammacoronavirus
Igacovirus
|
virus-isolate-R17/16
|
MN199463
Infectious-bronchitis-
MN199463
Gammacoronavirus
Igacovirus
|
virus-isolate-R17/19
|
MN199464
Infectious-bronchitis-
MN199464
Gammacoronavirus
Igacovirus
|
virus-isolate-R17/27
|
MN199465
Infectious-bronchitis-
MN199465
Gammacoronavirus
Igacovirus
|
virus-isolate-R17/36
|
MN199466
Infectious-bronchitis-
MN199466
Gammacoronavirus
Igacovirus
|
virus-isolate-R18/23
|
MT072864
Pangolin-coronavirus-
MT072864
Betacoronavirus
Sarbecovirus
|
isolate-PCoV_GX-P2V
|
MT072865
Pangolin-coronavirus-
MT072865
Betacoronavirus
Sarbecovirus
|
isolate-PCoV_GX-P3B-
|
genomic-sequence
|
MN128086
Infectious-bronchitis-
MN128086
Gammacoronavirus
Igacovirus
|
virus-strain-
|
TW2296/95 vac
|
MN128087
Infectious-bronchitis-
MN128087
Gammacoronavirus
Igacovirus
|
virus-strain-
|
TW2575/98 vac
|
MN128088
Infectious-bronchitis-
MN128088
Gammacoronavirus
Igacovirus
|
virus-strain-
|
TW2296/95w
|
NC_046954
Rodent-coronavirus-
NC_046954
unclassified
n/a
|
isolate-RtMruf-CoV-
Coronavirinae
|
2/JL2014
|
NC_046955
Shrew-coronavirus-
NC_046955
unclassified
n/a
|
isolate-Shrew-
Coronavirinae
|
CoV/Tibet2014-
|
ORF1ab-polyprotein,-
|
spike-glycoprotein,-
|
envelope-protein,-
|
membrane-protein,-and-
|
nucleocapsid-protein-
|
genes
|
NC_046965
Canada-goose-
NC_046965
Gammacoronavirus
unclassified
|
coronavirus-strain-
|
Cambridge_Bay_2017
|
MN307884
Infectious-bronchitis-
MN307884
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
ck/CH/LJX/2017/07
|
MT114538
Canine-coronavirus-
MT114538
Alphacoronavirus
Tegacovirus
|
strain-B639_ZJ_2019
|
MT114539
Canine-coronavirus-
MT114539
Alphacoronavirus
Tegacovirus
|
strain-B600_ZJ_2019
|
MT114540
Canine-coronavirus-
MT114540
Alphacoronavirus
Tegacovirus
|
strain-B447_ZJ_2019
|
MT114541
Canine-coronavirus-
MT114541
Alphacoronavirus
Tegacovirus
|
strain-B363_ZJ_2019
|
MT114542
Canine-coronavirus-
MT114542
Alphacoronavirus
Tegacovirus
|
strain-B203_GZ_2019
|
MT114543
Canine-coronavirus-
MT114543
Alphacoronavirus
Tegacovirus
|
strain-B194_GZ_2019
|
MT114544
Canine-coronavirus-
MT114544
Alphacoronavirus
Tegacovirus
|
strain-B135_JS_2018
|
MN794188
Infectious-bronchitis-
MN794188
Gammacoronavirus
Igacovirus
|
virus-strain-I0305/19
|
MN509587
Infectious-bronchitis-
MN509587
Gammacoronavirus
Igacovirus
|
virus-strain-
|
cK/CH/LDL/091021
|
MN509588
Infectious-bronchitis-
MN509588
Gammacoronavirus
Igacovirus
|
virus-strain-I0730/17
|
MN509589
Infectious-bronchitis-
MN509589
Gammacoronavirus
Igacovirus
|
virus-strain-
|
cK/CH/LHLJ/110664
|
MN517816
Infectious-bronchitis-
MN517816
Gammacoronavirus
Igacovirus
|
virus-strain-
|
cK/CH/LJL/090608
|
MN517817
Infectious-bronchitis-
MN517817
Gammacoronavirus
Igacovirus
|
virus-strain-
|
cK/CH/LSD/110723
|
MN531554
Infectious-bronchitis-
MN531554
Gammacoronavirus
Igacovirus
|
virus-strain-
|
cK/CH/LLN/120611
|
MN531555
Infectious-bronchitis-
MN531555
Gammacoronavirus
Igacovirus
|
virus-strain-
|
cK/CH/LSD/1112145
|
MN531556
Infectious-bronchitis-
MN531556
Gammacoronavirus
Igacovirus
|
virus-strain-
|
cK/CH/LSD/110856
|
NC_048212
Bat-coronavirus
NC_048212
unclassified
n/a
|
Coronavirinae
|
NC_048213
Infectious-bronchitis-
NC_048213
Gammacoronavirus
Igacovirus
|
virus-isolate-Ind-TN92-03
|
NC_048214
Duck-coronavirus-
NC_048214
Gammacoronavirus
Igacovirus
|
isolate-DK/GD/27/2014
|
NC_048216
NL63-related-bat-
NC_048216
Alphacoronavirus
Setracovirus
|
coronavirus-strain-
|
BtKYNL63-9b
|
NC_048217
Murine-hepatitis-virus-
NC_048217
Betacoronavirus
Embecovirus
|
strain-A59
|
MT337384
Bat-coronavirus-HKU9-
MT337384
Betacoronavirus
Nobecovirus
|
1-isolate-
|
MCL_20_Bat_76_10
|
MT337385
Coronavirus-BtRt-
MT337385
Betacoronavirus
Nobecovirus
|
BetaCoV/GX2018-
|
isolate-
|
MCL_20_Bat_76_8
|
MT337386
Coronavirus-BtRt-
MT337386
Betacoronavirus
Nobecovirus
|
BetaCoV/GX2018-
|
isolate-
|
MCL_19_Bat_606_2
|
MT337387
Coronavirus-BtRt-
MT337387
Betacoronavirus
Nobecovirus
|
BetaCoV/GX2018-
|
isolate-
|
MCL_19_Bat_606_10
|
MN548286
Infectious-bronchitis-
MN548286
Gammacoronavirus
Igacovirus
|
virus-strain-D1466
|
MN548287
Infectious-bronchitis-
MN548287
Gammacoronavirus
Igacovirus
|
virus-strain-H120
|
MN548288
Infectious-bronchitis-
MN548288
Gammacoronavirus
Igacovirus
|
virus-strain-Italy02
|
MN548289
Infectious-bronchitis-
MN548289
Gammacoronavirus
Igacovirus
|
virus-strain-QX
|
MN692770
Swine-enteric-
MN692770
Alphacoronavirus
Tegacovirus
|
coronavirus-isolate-
|
SeCoV-1480-Murcia-
|
Lorca
|
MT663548
Bat-alphacoronavirus-
MT663548
Alphacoronavirus
unclassified
|
isolate-AMA_L_F
|
MN548285
Infectious-bronchitis-
MN548285
Gammacoronavirus
Igacovirus
|
virus-strain-CR88
|
MN987230
Infectious-bronchitis-
MN987230
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
IBV/ck/EGY-
|
Monuf/NR725/16
|
MN987231
Infectious-bronchitis-
MN987231
Gammacoronavirus
Igacovirus
|
virus-isolate-
|
IBV/ck/EGY-
|
Monuf/USC-5/13
|
MW251308
Bat-coronavirus-
MW251308
Betacoronavirus
Sarbecovirus
|
RacCS203
|
|
A. Deduplication of Probes
Because the second set of virus inputs for developing the second iteration probes represented a less curated set of sequences, it was expected that there would be a very large degree of sequence homology across the various genomes. Therefore, the present probe set (first iteration probes and second iteration probes) was subjected to deduplication using the dedupe algorithm from the Joint Genome Institute as part of their BBTools suite of bioinformatic software tools. (jgi.doe.gov/data-and-tools/software-tools/bbtools/) The probe set was deduplicated using the Clustering by overlap method. In short, highly homologous probes were removed from the probe set and only the ‘best representative’ identified by the clustering algorithm were retained.
B. Specificity Check
The combination of probes comprising the first iteration probes and the second iteration probes is henceforth referred to as the v1 probe set. This probe set was then tested for theoretical pull-down efficacy against the entirety of the coronavirus isolate sequences that we had downloaded from NCBI in 2020, which contained ˜2700 sequences. Theoretical pulldown was calculated using both high and low stringency assumptions, which consisted of 70% minimum identity over 30 bp for low and 90% minimum identity over 70 bp for high stringency. Using the higher stringency requirements, a gap analysis of the sequences (i.e., the sequences expected *not* to be captured) was generated.
C. Probe Set backfill
From this analysis a BED file was generated, and all gaps in sequences were used as input regions for the proprietary algorithm to generate another set of probes to backfill regions that for any reason were not showing up as ‘covered’ in the analysis. In many cases, only 1 or a few extra probes were added per genome. See Table 3. This probe set was then subjected to the deduplication step as outlined above. SEQ ID NOs: 1-22909 resulted from this analysis.
TABLE 3
|
|
Accession
Name
Accession
Name
|
|
KF636752
Bat Hp-
KP202848
Transmissible gastroenteritis virus
|
betacoronavirus/Zhejiang2013
isolate SHXB
|
KM347965
Ferret coronavirus isolate FRCoV-
KP780179
Infectious bronchitis virus strain
|
NL-2010
gammaCoV/Ck/Italy/I2022/13
|
KX512809
Ferret enteric coronavirus strain
KP849472
Alphacoronavirus 1 strain 23/03
|
FECV1
|
KX512810
Ferret systemic coronavirus strain
KP886808
Bat SARS-like coronavirus
|
FSCV6
YNLF_31C
|
HM245925
Mink coronavirus strain WD1127
KP886809
Bat SARS-like coronavirus
|
YNLF_34C
|
KY073745
NL63-related bat coronavirus
KR608272
Infectious bronchitis virus isolate
|
strain BtKYNL63-9b
LDT3-A
|
KY073746
NL63-related bat coronavirus
KR822424
European turkey coronavirus 080385d
|
strain BtKYNL63-15
|
KJ473809
BtNv-AlphaCoV/SC2013
KT368891
Camel coronavirus HKU23 isolate
|
camel/Riyadh/Ry123/2015
|
KU762338
Rousettus bat coronavirus isolate
KT444582
SARS-like coronavirus WIV16
|
GCCDC1 356
|
MK720944
Tylonycteris bat coronavirus
KT696544
Transmissible gastroenteritis virus
|
HKU33 strain GZ151867
strain JS2012
|
MF113046
Alphacoronavirus
KT852992
Infectious bronchitis virus isolate
|
Mink/China/1/2016
tl/CH/LDT3/03
|
MH938449
Alphacoronavirus Bat-
KU131570
Human coronavirus OC43 strain
|
CoV/P.kuhlii/Italy/3398-19/2015
HCoV-OC43/UK/London/2011
|
MH938450
Alphacoronavirus Bat-
KU182964
Bat coronavirus isolate JTMC15
|
CoV/P.kuhlii/Italy/206679-3/2010
|
MH938448
Alphacoronavirus Bat-
KU215419
Feline coronavirus isolate inoculum
|
CoV/P.kuhlii/Italy/206645-
|
41/2011
|
HM245926
Mink coronavirus strain WD1133
KU215420
Feline coronavirus isolate Cat1_day7
|
MG916901
Bat coronavirus
KU215421
Feline coronavirus isolate
|
BtCoV/Rh/YN2012 isolate
Cat2_day21_deletion
|
BtCoV/Rh/YN2012_Rs3376
|
MG916902
Bat coronavirus
KU215422
Feline coronavirus isolate
|
BtCoV/Rh/YN2012 isolate
Cat2_day21_withoutdeletion
|
BtCoV/Rh/YN2012_Rs4125
|
MG916903
Bat coronavirus
KU215423
Feline coronavirus isolate Cat3_day9
|
BtCoV/Rh/YN2012 isolate
|
BtCoV/Rh/YN2012_Rs4259
|
MG916904
Bat coronavirus
KU215424
Feline coronavirus isolate
|
BtCoV/Rh/YN2012 isolate
Cat1_day28_deletion
|
BtCoV/Rh/YN2012_Ra13591
|
MK492263
Bat coronavirus strain BtCoV92
KU215425
Feline coronavirus isolate
|
Cat1_day28_withoutdeletion
|
MN197549
Infectious bronchitis virus isolate
KU215426
Feline coronavirus isolate Cat2_day84
|
CK/CH/GD/GDTS13
|
MN512434
Infectious bronchitis virus isolate
KU215427
Feline coronavirus isolate
|
IBV/Ck/Can/17-035614
Cat3_day28_deletion
|
MN512435
Infectious bronchitis virus isolate
KU215428
Feline coronavirus isolate
|
IBV/Ck/Can/17-036989
Cat3_day28_withoutdeletion
|
MN512436
Infectious bronchitis virus isolate
KU291448
Human coronavirus 229E isolate
|
IBV/Ck/Can/18-048192T
HCoV-229E/BN1/GER/2015
|
MN512437
Infectious bronchitis virus isolate
KU361188
Infectious bronchitis virus isolate
|
IBV/Ck/Can/18-048430
CK/CH/2014/QL1403
|
MN512438
Infectious bronchitis virus isolate
KU558922
Betacoronavirus 1 isolate Buffalo
|
IBV/Ck/Can/18-049707
coronavirus B1-24F
|
MN096598
Infectious bronchitis virus strain
KU558923
Betacoronavirus 1 isolate Buffalo
|
ck/CH/YNSL/160501
coronavirus B1-28F
|
MK581202
Infectious bronchitis virus strain
KU729220
Transmissible gastroenteritis virus
|
gammaCoV/Ck/Poland/80/1989
strain TH-98
|
MK581204
Infectious bronchitis virus strain
KU762338
Rousettus bat coronavirus isolate
|
gammaCoV/Ck/Poland/255/1997
GCCDC1 356
|
MK329221
Infectious bronchitis virus isolate
KU886219
Bovine coronavirus strain BCV-AKS-
|
CK-CH-GX-YL17
01
|
MK309398
Infectious bronchitis virus isolate
KU973692
UNVERIFIED: SARS-related
|
CK/CH/GD/HY16
coronavirus isolate F46
|
MK217372
Infectious bronchitis virus strain
KX083668
Transmissible gastroenteritis virus
|
I0221/17
isolate HE-1
|
MK217373
Infectious bronchitis virus strain
KX185057
Infectious bronchitis virus strain
|
I0725/17
ck/CH/LHLJ/95I
|
MK217374
Infectious bronchitis virus strain
KX252791
Infectious bronchitis virus strain
|
I0916/16
ck/CH/LLN/98I
|
MK217375
Infectious bronchitis virus strain
KX266757
Infectious bronchitis virus isolate
|
I1209/16
3575/08
|
MK574042
Infectious bronchitis virus strain
KX344031
Human coronavirus OC43 isolate
|
ck/CH/LHB/110615
LRTI_238
|
MK574043
Infectious bronchitis virus strain
KX432213
Canine respiratory coronavirus strain
|
ck/CH/LHB/110617
BJ232
|
MK142676
Infectious bronchitis virus isolate
KX499468
Transmissible gastroenteritis virus
|
ahysx-1
strain TGEV AHHF
|
MK878536
Infectious bronchitis virus isolate
KX512809
Ferret enteric coronavirus strain
|
GA9977/2019
FECV1
|
MH924835
Infectious bronchitis virus strain
KX512810
Ferret systemic coronavirus strain
|
gammaCoV/ck/China/I0636/16
FSCV6
|
MH878976
Infectious bronchitis virus isolate
KX538964
Human coronavirus OC43 isolate MY-
|
VFAR-047
U002/12
|
MG021194
Infectious bronchitis virus isolate
KX538965
Human coronavirus OC43 isolate MY-
|
gammaCoV/AvCov/Ck/Italy/624I/
U208/12
|
96
|
MH021175
Avian coronavirus strain D274
KX538966
Human coronavirus OC43 isolate MY-
|
U236/12
|
MG448607
Infectious bronchitis virus strain
KX538967
Human coronavirus OC43 isolate MY-
|
ck/CH/LHB/121042
U413/12
|
MF882923
Infectious bronchitis virus isolate
KX538968
Human coronavirus OC43 isolate MY-
|
CK/CH/HB/2016
U464/12
|
MF421319
Infectious bronchitis virus isolate
KX538969
Human coronavirus OC43 isolate MY-
|
UY/09/CA/01
U523/12
|
KX252791
Infectious bronchitis virus strain
KX538970
Human coronavirus OC43 isolate MY-
|
ck/CH/LLN/98I
U710/12
|
KX185057
Infectious bronchitis virus strain
KX538971
Human coronavirus OC43 isolate MY-
|
ck/CH/LHLJ/95I
U732/12
|
KX266757
Infectious bronchitis virus isolate
KX538972
Human coronavirus OC43 isolate MY-
|
3575/08
U774/12
|
KU361188
Infectious bronchitis virus isolate
KX538973
Human coronavirus OC43 isolate MY-
|
CK/CH/2014/QL1403
U868/12
|
KR608272
Infectious bronchitis virus isolate
KX538974
Human coronavirus OC43 isolate MY-
|
LDT3-A
U945/12
|
KT852992
Infectious bronchitis virus isolate
KX538975
Human coronavirus OC43 isolate MY-
|
tl/CH/LDT3/03
U1024/12
|
KR822424
European turkey coronavirus
KX538976
Human coronavirus OC43 isolate MY-
|
080385d
U1057/12
|
KP780179
Infectious bronchitis virus strain
KX538977
Human coronavirus OC43 isolate MY-
|
gammaCoV/Ck/Italy/I2022/13
U1140/12
|
KM454473
Duck coronavirus isolate
KX538978
Human coronavirus OC43 isolate MY-
|
DK/GD/27/2014
U1758/13
|
KF460437
Infectious bronchitis virus isolate
KX538979
Human coronavirus OC43 isolate MY-
|
VicS-v
U1975/13
|
KF931628
Infectious bronchitis virus isolate
KX574227
Bat coronavirus isolate
|
VicS-del
PREDICT/PDF-2180
|
KM213963
Infectious bronchitis virus isolate
KX722529
Feline coronavirus isolate UG-FH8
|
CK/CH/XDC- 2/2013
|
KF663559
Infectious bronchitis virus isolate
KX722530
Feline coronavirus isolate Cat 1
|
ck/CH/IBTZ/2012
Karlslunde
|
KF668605
Infectious bronchitis virus isolate
KX900393
Transmissible gastroenteritis virus
|
CK/CH/SD09/005
strain TGEV/USA/Z/1986
|
JX897900
UNVERIFIED: Infectious
KX900394
Transmissible gastroenteritis virus
|
bronchitis virus isolate GX-
strain TGEV/USA/HB/1988
|
NN09032
|
JQ977697
Infectious bronchitis virus isolate
KX900395
Transmissible gastroenteritis virus
|
SNU8067
strain TGEV/USA/Minnesota138/2006
|
GU393332
Infectious bronchitis virus serotype
KX900396
Transmissible gastroenteritis virus
|
Delaware 072
strain TGEV/USA/Illinois139/2006
|
JF330898
Infectious bronchitis virus strain
KX900397
Transmissible gastroenteritis virus
|
ck/CH/LHB/100801
strain
|
TGEV/USA/NorthCarolina140/2007
|
GQ504722
Infectious bronchitis virus strain
KX900398
Transmissible gastroenteritis virus
|
Georgia 1998 pass8
strain TGEV/USA/Minnesota141/2007
|
GQ504723
Infectious bronchitis virus strain
KX900399
Transmissible gastroenteritis virus
|
Georgia 1998 Vaccine
strain
|
TGEV/USA/NorthCarolina142/2007
|
GQ427173
Turkey coronavirus strain
KX900400
Transmissible gastroenteritis virus
|
TCoV/VA-74/03
strain TGEV/USA/Iowa143/2008
|
GQ427174
Turkey coronavirus strain
KX900401
Transmissible gastroenteritis virus
|
TCoV/TX-GL/01
strain TGEV/USA/Tennessee144/2008
|
GQ427175
Turkey coronavirus strain
KX900402
Transmissible gastroenteritis virus
|
TCoV/IN-517/94
strain TGEV/Mex/145/2008
|
GQ427176
Turkey coronavirus strain
KX900403
Transmissible gastroenteritis virus
|
TCoV/TX-1038/98
strain TGEV/USA/Illinois146/2008
|
EU022525
Turkey coronavirus isolate
KX900404
Transmissible gastroenteritis virus
|
TCoV-540
strain TGEV/USA/Oklahoma147/2012
|
EU022526
Turkey coronavirus isolate
KX900405
Transmissible gastroenteritis virus
|
TCoV-ATCC
strain TGEV/USA/Minnesota148/2013
|
EU095850
Turkey coronavirus isolate MG10
KX900406
Transmissible gastroenteritis virus
|
strain TGEV/USA/Illinois149/2013
|
EU526388
Infectious bronchitis virus strain
KX900407
Transmissible gastroenteritis virus
|
A2
strain TGEV/USA/Minnesota150/2013
|
EU111742
Coronavirus SW1
KX900408
Transmissible gastroenteritis virus
|
strain TGEV/USA/Wisconsin151/2014
|
AY646283
Avian infectious bronchitis virus
KX900409
Transmissible gastroenteritis virus
|
partridge/GD/S14/2003
strain TGEV/USA/Minnesota152/2014
|
AY319651
Avian infectious bronchitis virus
KX900410
Transmissible gastroenteritis virus
|
isolate BJ
strain TGEV/USA/Minnesota153/2014
|
MH532440
Quail deltacoronavirus strain
KX900411
Transmissible gastroenteritis virus
|
G032/2015
strain
|
TGEV/USA/SouthDakota154/2014
|
MG812377
Sparrow deltacoronavirus strain
KX964649
Coronavirus AcCoV-JC34
|
ISU42824
|
MG812378
Sparrow deltacoronavirus strain
KX982264
Bovine coronavirus isolate
|
ISU73347
BCoV_2014_13
|
JQ065044
White-eye coronavirus HKU16
KY014281
Human coronavirus OC43 isolate
|
strain HKU16-6847
2002-04
|
JQ065045
Sparrow coronavirus HKU17
KY014282
Human coronavirus OC43 isolate
|
strain HKU17-6124
2007-09
|
JQ065046
Magpie-robin coronavirus HKU18
KY073744
NL63-related bat coronavirus strain
|
strain HKU18-chu3
BtKYNL63-9a
|
JQ065047
Night-heron coronavirus HKU19
KY073745
NL63-related bat coronavirus strain
|
strain HKU19-6918
BtKYNL63-9b
|
JQ065048
Wigeon coronavirus HKU20 strain
KY073746
NL63-related bat coronavirus strain
|
HKU20-9243
BtKYNL63-15
|
JQ065049
Common-moorhen coronavirus
KY073747
229E-related bat coronavirus strain
|
HKU21 strain HKU21-8295
BtKY229E-1
|
FJ376620
Bulbul coronavirus HKU11-796
KY073748
229E-related bat coronavirus strain
|
BtKY229E-8
|
FJ376621
Thrush coronavirus HKU12-600
KY292377
Feline coronavirus strain
|
HLJ/DQ/2016/01
|
FJ376622
Munia coronavirus HKU13-3514
KY352407
Severe acute respiratory syndrome-
|
related coronavirus strain BtKY72
|
AF220295
Bovine coronavirus strain Quebec
KY369905
Human coronavirus OC43 strain
|
SC831
|
AF391542
Bovine coronavirus isolate BCoV-
KY369906
Human coronavirus OC43 strain
|
LUN
SC622
|
AY319651
Avian infectious bronchitis virus
KY369907
Human coronavirus OC43 strain
|
isolate BJ
SC9741
|
AY391777
Human coronavirus OC43
KY369908
Human coronavirus 229E strain SC579
|
AY585228
Human coronavirus OC43 strain
KY369909
Human coronavirus 229E strain SC677
|
ATCC VR-759
|
AY585229
Human coronavirus OC43 serotype
KY369910
Human coronavirus 229E strain
|
OC43-Paris
SC1143
|
AY646283
Avian infectious bronchitis virus
KY369911
Human coronavirus 229E strain
|
partridge/GD/S14/2003
SC1212
|
AY884001
Human coronavirus HKU1
KY369912
Human coronavirus 229E strain
|
genotype B
SC9731
|
AY903459
Human coronavirus OC43 strain
KY369913
Human coronavirus 229E strain
|
87309 Belgium 2003
SC1073
|
AY903460
Human coronavirus OC43 strain
KY369914
Human coronavirus 229E strain
|
19572 Belgium 2004
SC9773
|
DQ011855
Porcine hemagglutinating
KY417142
Bat SARS-like coronavirus isolate
|
encephalomyelitis virus strain
As6526
|
VW572
|
DQ022305
Bat SARS coronavirus HKU3-1
KY417143
Bat SARS-like coronavirus isolate
|
Rs4081
|
DQ071615
Bat SARS coronavirus Rp3
KY417144
Bat SARS-like coronavirus isolate
|
Rs4084
|
DQ084199
bat SARS coronavirus HKU3-2
KY417145
Bat SARS-like coronavirus isolate
|
Rf4092
|
DQ084200
bat SARS coronavirus HKU3-3
KY417147
Bat SARS-like coronavirus isolate
|
Rs4237
|
DQ201447
Transmissible gastroenteritis virus
KY417148
Bat SARS-like coronavirus isolate
|
strain TS
Rs4247
|
DQ339101
Human coronavirus HKU1 strain
KY417149
Bat SARS-like coronavirus isolate
|
N5P8 genotype A/B recombinant
Rs4255
|
DQ412042
Bat SARS coronavirus Rf1
KY417150
Bat SARS-like coronavirus isolate
|
Rs4874
|
DQ412043
Bat SARS coronavirus Rm1
KY417151
Bat SARS-like coronavirus isolate
|
Rs7327
|
DQ415897
Human coronavirus HKU1 strain
KY419103
Porcine hemagglutinating
|
N20 genotype C
encephalomyelitis virus strain PHEV
|
CoV USA-15TOSU25049
|
DQ415898
Human coronavirus HKU1 strain
KY419104
Porcine hemagglutinating
|
N21 genotype C
encephalomyelitis virus strain PHEV
|
CoV USA-15TOSU0331
|
DQ415899
Human coronavirus HKU1 strain
KY419105
Porcine hemagglutinating
|
N22 genotype C
encephalomyelitis virus strain PHEV
|
CoV USA-15TOSU0582
|
DQ415902
Human coronavirus HKU1 strain
KY419106
Porcine hemagglutinating
|
N25 genotype B
encephalomyelitis virus strain PHEV
|
CoV USA-15TOSU1785
|
DQ415911
Human coronavirus HKU1 strain
KY419107
Porcine hemagglutinating
|
N15 genotype B
encephalomyelitis virus strain PHEV
|
CoV USA-15TOSU1209
|
DQ415912
Human coronavirus HKU1 strain
KY419109
Porcine hemagglutinating
|
N16 genotype C
encephalomyelitis virus strain PHEV
|
CoV USA-15TOSU1655
|
DQ415913
Human coronavirus HKU1 strain
KY419110
Porcine hemagglutinating
|
N17 genotype C
encephalomyelitis virus strain PHEV
|
CoV USA-15TOSU1362
|
DQ443743
Transmissible gastroenteritis virus
KY419112
Porcine hemagglutinating
|
strain SC-Y
encephalomyelitis virus strain PHEV
|
CoV USA-15TOSU1765
|
DQ646405
Infectious bronchitis virus isolate
KY419113
Porcine hemagglutinating
|
TW2575/98
encephalomyelitis virus strain PHEV
|
CoV USA-15TOSU1582
|
DQ648856
Bat coronavirus (BtCoV/273/2005)
KY554972
Human coronavirus OC43 strain N07-
|
1541B_433X
|
DQ648857
Bat coronavirus (BtCoV/279/2005)
KY554973
Human coronavirus OC43 strain N07-
|
1689B_116X
|
DQ811784
Bovine coronavirus DB2
KY554974
Human coronavirus OC43 strain N08-
|
33B_360X
|
DQ811785
TGEV Miller M6
KY554975
Human coronavirus OC43 strain N09-
|
382B
|
DQ811786
TGEV Miller M60
KY566209
Feline coronavirus strain
|
HLJ/HRB/2016/10
|
DQ811788
TGEV Purdue P115
KY566210
Feline coronavirus strain
|
HLJ/HRB/2016/11
|
DQ811789
TGEV virulent Purdue
KY566211
Feline coronavirus strain
|
HLJ/HRB/2016/13
|
DQ848678
Feline coronavirus strain FCoV
KY621348
Human coronavirus 229E strain SC379
|
C1Je
|
DQ915164
Bovine coronavirus isolate Alpaca
KY674914
Human coronavirus 229E strain SC399
|
EF065513
Bat coronavirus HKU9-1
KY674917
Human coronavirus OC43 strain N07-
|
1609B
|
EF065514
Bat coronavirus HKU9-2
KY674918
Human coronavirus OC43 strain N07-
|
1647B
|
EF065516
Bat coronavirus HKU9-4
KY674920
Human coronavirus OC43 strain N09-
|
595B
|
EF203064
Bat coronavirus HKU2 strain
KY674921
Human coronavirus HKU1 genotype B
|
HKU2/GD/430/2006
strain N08-87
|
EF203065
Bat coronavirus HKU2 strain
KY684759
Human coronavirus OC43 strain
|
HKU2/HK/46/2006
SC2269
|
EF203066
Bat coronavirus HKU2 strain
KY684760
Human coronavirus 229E strain
|
HKU2/HK/298/2006
SC2282
|
EF203067
Bat coronavirus HKU2 strain
KY770858
Bat coronavirus isolate Anlong-103
|
HKU2/HK/33/2006
|
EF424615
Bovine coronavirus E-AH65
KY770859
Bat coronavirus isolate Anlong-112
|
EF424616
Bovine coronavirus E-AH65-TC
KY770860
Bat coronavirus isolate Jiyuan-84
|
EF424617
Bovine coronavirus R-AH65
KY799179
Myotis lucifugus coronavirus
|
EF424618
Bovine coronavirus R-AH65-TC
KY938558
Bat coronavirus strain 16BO133
|
EF424619
Bovine coronavirus E-AH187
KY967356
Human coronavirus OC43 strain
|
SC2924
|
EF424620
Bovine coronavirus R-AH187
KY967357
Human coronavirus 229E strain
|
SC2872
|
EF424621
Sable antelope coronavirus
KY967358
Human coronavirus OC43 strain
|
US/OH1/2003
SC2770
|
EF424622
Giraffe coronavirus US/OH3-
KY967359
Human coronavirus OC43 strain
|
TC/2006
SC2730
|
EF424623
Giraffe coronavirus US/OH3/2003
KY967360
Human coronavirus OC43 strain
|
SC2476
|
EF424624
Calf-giraffe coronavirus
KY967361
Human coronavirus OC43 strain
|
US/OH3/2006
SC2345
|
EF446615
Equine coronavirus strain NC99
KY983583
Human coronavirus OC43 strain
|
SC2481
|
EU022525
Turkey coronavirus isolate TCoV-
KY983585
Human coronavirus OC43 strain
|
540
SC2854
|
EU022526
Turkey coronavirus isolate TCoV-
KY983587
Human coronavirus 229E strain
|
ATCC
SC3112
|
EU074218
Transmissible gastroenteritis virus
KY983588
Human coronavirus OC43 strain
|
strain attenuated H
SC3118
|
EU095850
Turkey coronavirus isolate MG10
KY994645
Porcine hemagglutinating
|
encephalomyelitis virus strain JL/2008
|
EU111742
Coronavirus SW1
KY996417
Human coronavirus 229E strain
|
229E/UF-1/2016
|
EU186072
Feline coronavirus isolate Black
MF083115
Porcine hemagglutinating
|
encephalomyelitis virus strain CC14
|
EU420137
Bat coronavirus 1B strain
MF094685
Swine acute diarrhea syndrome related
|
AFCD307
coronavirus isolate 8462
|
EU420138
Miniopterus bat coronavirus 1
MF094686
Swine acute diarrhea syndrome related
|
coronavirus isolate 8495
|
EU420139
Bat coronavirus HKU8 strain
MF113046
Alphacoronavirus Mink/China/1/2016
|
AFCD77
|
EU526388
Infectious bronchitis virus strain
MF314143
Human coronavirus OC43 isolate
|
A2
HCoV-OC43/USA/ACRI_0052/2016
|
FJ376619
Bulbul coronavirus HKU11-934
MF374983
Human coronavirus OC43 isolate
|
HCoV-OC43/USA/TCNP_0070/2016
|
FJ376620
Bulbul coronavirus HKU11-796
MF374984
Human coronavirus OC43 isolate
|
HCoV-OC43/USA/TCNP_00204/2017
|
FJ376621
Thrush coronavirus HKU12-600
MF374985
Human coronavirus OC43 isolate
|
HCoV-OC43/USA/TCNP_00212/2017
|
FJ376622
Munia coronavirus HKU13-3514
MF421319
Infectious bronchitis virus isolate
|
UY/09/CA/01
|
FJ415324
Human enteric coronavirus 4408
MF542265
Human coronavirus 229E strain
|
229E/Haiti-1/2016
|
FJ425184
Waterbuck coronavirus US/OH-
MF577027
Porcine epidemic diarrhea virus strain
|
WD358-TC/1994
PEDV/Belgorod/dom/2008
|
FJ425185
Waterbuck coronavirus US/OH-
MF593268
Middle East respiratory syndrome-
|
WD358-GnC/1994
related coronavirus strain
|
Neoromicia/5038
|
FJ425186
Waterbuck coronavirus US/OH-
MF882923
Infectious bronchitis virus isolate
|
WD358/1994
CK/CH/HB/2016
|
FJ425187
White-tailed deer coronavirus
MG021194
Infectious bronchitis virus isolate
|
US/OH-WD470/1994
gammaCoV/AvCov/Ck/Italy/624I/96
|
FJ425188
Sambar deer coronavirus US/OH-
MG021451
Middle East respiratory syndrome-
|
WD388-TC/1994
related coronavirus isolate NL13845
|
FJ425189
Sambar deer coronavirus US/OH-
MG021452
Middle East respiratory syndrome-
|
WD388/1994
related coronavirus isolate NL140422
|
FJ425190
Sambar deer coronavirus US/OH-
MG197709
Human coronavirus OC43 strain BJ-
|
WD388-TC/1994 calf-passaged
112
|
FJ588686
Bat SARS CoV Rs672/2006
MG197710
Human coronavirus OC43 strain BJ-
|
124
|
FJ647223
Murine coronavirus MHV-1
MG197711
Human coronavirus OC43 strain BJ-
|
164
|
FJ755618
Transmissible gastroenteritis virus
MG197712
Human coronavirus OC43 strain BJ-
|
strain H16
165
|
FJ938051
Feline coronavirus RM
MG197713
Human coronavirus OC43 strain BJ-
|
221
|
FJ938052
Feline coronavirus UU11
MG197714
Human coronavirus OC43 strain CC-
|
23
|
FJ938053
Feline coronavirus UU7
MG197715
Human coronavirus OC43 strain
|
GZYF-26
|
FJ938054
Feline coronavirus UU4
MG197716
Human coronavirus OC43 strain WZ-
|
303
|
FJ938055
Feline coronavirus UU8
MG197717
Human coronavirus OC43 strain WZ-
|
522
|
FJ938056
Feline coronavirus UU5
MG197718
Human coronavirus OC43 strain YC-
|
55
|
FJ938057
Feline coronavirus UU15
MG197719
Human coronavirus OC43 strain YC-
|
67
|
FJ938058
Feline coronavirus UU16
MG197720
Human coronavirus OC43 strain YC-
|
68
|
FJ938059
Feline coronavirus UU10
MG197721
Human coronavirus OC43 strain YC-
|
72
|
FJ938060
Feline coronavirus UU2
MG197722
Human coronavirus OC43 strain YC-
|
207
|
FJ938061
Feline coronavirus UU3
MG197723
Human coronavirus OC43 strain HZ-
|
459
|
FJ938062
Feline coronavirus UU9
MG448607
Infectious bronchitis virus strain
|
ck/CH/LHB/121042
|
FJ938063
Bovine coronavirus E-DB2-TC
MG518518
Water deer coronavirus isolate W17-18
|
FJ938064
Bovine coronavirus E-AH187-TC
MG596802
Middle East respiratory syndrome-
|
related coronavirus isolate Bat-
|
CoV/H.savii/Italy/206645-40/2011
|
FJ938065
Bovine respiratory coronavirus
MG596803
Middle East respiratory syndrome-
|
AH187
related coronavirus isolate Bat-
|
CoV/P.khulii/Italy/206645-63/2011
|
FJ938066
Bovine respiratory coronavirus
MG757138
Bovine coronavirus isolate ICSA21-
|
bovine/US/OH-440-TC/1996
LBA
|
FJ938067
Human enteric coronavirus strain
MG757139
Bovine coronavirus isolate ICSA16-
|
4408
EN
|
FJ938068
Rat coronavirus Parker
MG757140
Bovine coronavirus isolate ICSA16-
|
LBA
|
GQ152141
Feline coronavirus strain
MG757141
Bovine coronavirus isolate ICSA-pool-
|
FCoV/NTU156/P/2007
EN
|
GQ153539
Bat SARS coronavirus HKU3-4
MG757142
Bovine coronavirus isolate ICSA-pool-
|
LBA
|
GQ153540
Bat SARS coronavirus HKU3-5
MG762674
Rousettus bat coronavirus HKU9
|
isolate Rousettus spp/Jinghong/2009
|
GQ153541
Bat SARS coronavirus HKU3-6
MG772933
Bat SARS-like coronavirus isolate bat-
|
SL-CoVZC45
|
GQ153542
Bat SARS coronavirus HKU3-7
MG772934
Bat SARS-like coronavirus isolate bat-
|
SL-CoVZXC21
|
GQ153543
Bat SARS coronavirus HKU3-8
MG812377
Sparrow deltacoronavirus strain
|
ISU42824
|
GQ153544
Bat SARS coronavirus HKU3-9
MG812378
Sparrow deltacoronavirus strain
|
ISU73347
|
GQ153545
Bat SARS coronavirus HKU3-10
MG893511
Feline coronavirus isolate Felix
|
GQ153546
Bat SARS coronavirus HKU3-11
MG916901
Bat coronavirus BtCoV/Rh/YN2012
|
isolate BtCoV/Rh/YN2012_Rs3376
|
GQ153547
Bat SARS coronavirus HKU3-12
MG916902
Bat coronavirus BtCoV/Rh/YN2012
|
isolate BtCoV/Rh/YN2012_Rs4125
|
GQ153548
Bat SARS coronavirus HKU3-13
MG916903
Bat coronavirus BtCoV/Rh/YN2012
|
isolate BtCoV/Rh/YN2012_Rs4259
|
GQ427173
Turkey coronavirus strain
MG916904
Bat coronavirus BtCoV/Rh/YN2012
|
TCoV/VA-74/03
isolate BtCoV/Rh/YN2012_Ra13591
|
GQ427174
Turkey coronavirus strain
MG977444
Human coronavirus OC43 isolate TNP
|
TCoV/TX-GL/01
F1778_2
|
GQ427175
Turkey coronavirus strain
MG977445
Human coronavirus OC43 isolate TNP
|
TCoV/IN-517/94
F1790_2
|
GQ427176
Turkey coronavirus strain
MG977447
Human coronavirus OC43 isolate TNP
|
TCoV/TX-1038/98
F1832_2
|
GQ477367
Canine coronavirus strain
MG977449
Human coronavirus OC43 isolate TNP
|
CCoV/NTU336/F/2008
F1834_2
|
GQ504722
Infectious bronchitis virus strain
MG977451
Human coronavirus OC43 isolate TNP
|
Georgia 1998 pass8
12636
|
GQ504723
Infectious bronchitis virus strain
MG977452
Human coronavirus OC43 isolate TNP
|
Georgia 1998 Vaccine
12643
|
GU190215
Bat coronavirus BM48-
MH021175
Avian coronavirus strain D274
|
31/BGR/2008
|
GU393332
Infectious bronchitis virus serotype
MH043952
Bovine coronavirus isolate 4-17-03
|
Delaware 072
|
GU553361
Feline coronavirus UU22 isolate
MH043953
Bovine coronavirus isolate 4-17-25
|
TCVSP-ROTTIER-00022
|
GU553362
Feline coronavirus UU23 isolate
MH043954
Bovine coronavirus isolate 4-17-08
|
TCVSP-ROTTIER-00023
|
HM211099
Bat coronavirus HKU9-5-2
MH043955
Bovine coronavirus isolate 7-16-23
|
HM211101
Bat coronavirus HKU9-10-2
MH121121
Human coronavirus OC43 isolate
|
HCoV-OC43/USA/ACRI_0213/2016
|
HM245925
Mink coronavirus strain WD1127
MH532440
Quail deltacoronavirus strain
|
G032/2015
|
HM245926
Mink coronavirus strain WD1133
MH687935
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_24
|
HM776941
Transmissible gastroenteritis virus
MH687936
Alphacoronavirus sp. strain
|
isolate AYU
VZ_AlphaCoV_16715_31
|
HQ012367
Feline coronavirus UU17
MH687937
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_32
|
HQ012368
Feline coronavirus UU18
MH687939
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_39_c2
|
HQ012369
Feline coronavirus UU21
MH687940
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_45
|
HQ012370
Feline coronavirus UU24
MH687942
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_47_c2
|
HQ012371
Feline coronavirus UU31
MH687943
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_5
|
HQ012372
Feline coronavirus UU34
MH687944
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_53
|
HQ392469
Feline coronavirus UU40
MH687947
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_63
|
HQ392470
Feline coronavirus UU19
MH687949
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_76
|
HQ392471
Feline coronavirus UU20
MH687950
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_77
|
HQ392472
Feline coronavirus UU30
MH687951
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16715_78
|
HQ462571
Transmissible gastroenteritis virus
MH687952
Alphacoronavirus sp. strain
|
strain WH-1
VZ_AlphaCoV_16715_84
|
JF330898
Infectious bronchitis virus strain
MH687953
Alphacoronavirus sp. strain
|
ck/CH/LHB/100801
VZ_AlphaCoV_16715_86
|
JF792616
Rat coronavirus isolate 681
MH687955
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16845_47
|
JF792617
Rat coronavirus isolate 8190
MH687956
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_16845_53
|
JN129834
Human coronavirus OC43 strain
MH687957
Alphacoronavirus sp. strain
|
HK04-01
VZ_AlphaCoV_16845_64
|
JN129835
Human coronavirus OC43 strain
MH687958
Alphacoronavirus sp. strain
|
HK04-02
VZ_AlphaCoV_16845_87
|
JN183882
Feline coronavirus UU47
MH687959
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_17819_17
|
JN183883
Feline coronavirus UU54
MH687960
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_17819_22
|
JN856008
Canine coronavirus strain A76
MH687961
Alphacoronavirus sp. strain
|
VZ_AlphaCoV_17819_4
|
JQ065044
White-eye coronavirus HKU16
MH687962
Alphacoronavirus sp. strain
|
strain HKU16-6847
VZ_AlphaCoV_17819_50
|
JQ065045
Sparrow coronavirus HKU17
MH687965
Alphacoronavirus sp. strain
|
strain HKU17-6124
VZ_AlphaCoV_20745_17
|
JQ065046
Magpie-robin coronavirus HKU18
MH687966
Alphacoronavirus sp. strain
|
strain HKU18-chu3
VZ_AlphaCoV_20745_6
|
JQ065047
Night-heron coronavirus HKU19
MH687968
Betacoronavirus sp. strain
|
strain HKU19-6918
VZ_BetaCoV_16715_52
|
JQ065048
Wigeon coronavirus HKU20 strain
MH687970
Betacoronavirus sp. strain
|
HKU20-9243
VZ_BetaCoV_20724_34_c12
|
JQ065049
Common-moorhen coronavirus
MH810163
Yak coronavirus strain
|
HKU21 strain HKU21-8295
YAK/HY24/CH/2017
|
JQ977697
Infectious bronchitis virus isolate
MH817484
Feline Alphacoronavirus 1 strain
|
SNU8067
FCoV-SB22
|
JQ989270
Rousettus bat coronavirus HKU10
MH878976
Infectious bronchitis virus isolate
|
isolate 183A
VFAR-047
|
JQ989271
Rousettus bat coronavirus HKU10
MH924835
Infectious bronchitis virus strain
|
isolate 175A
gammaCoV/ck/China/I0636/16
|
JQ989272
Hipposideros bat coronavirus
MH938448
Alphacoronavirus Bat-
|
HKU10 isolate TLC1343A
CoV/P.kuhlii/Italy/206645-41/2011
|
JQ989273
Hipposideros bat coronavirus
MH938449
Alphacoronavirus Bat-
|
HKU10 isolate TLC1347A
CoV/P.kuhlii/Italy/3398-19/2015
|
JX860640
Canine respiratory coronavirus
MH938450
Alphacoronavirus Bat-
|
strain K37
CoV/P.kuhlii/Italy/206679-3/2010
|
JX897900
UNVERIFIED: Infectious
MH940245
Human coronavirus HKU1 isolate
|
bronchitis virus isolate GX-
SI17244
|
NN09032
|
JX993987
Bat coronavirus Rp/Shaanxi2011
MK142676
Infectious bronchitis virus isolate
|
ahysx-1
|
JX993988
Bat coronavirus Cp/Yunnan2011
MK211369
Coronavirus BtSk-
|
AlphaCoV/GX2018A
|
KC869678
Coronavirus Neoromicia/PML-
MK211370
Coronavirus BtSk-
|
PHE1/RSA/2011
AlphaCoV/GX2018B
|
KC881005
Bat SARS-like coronavirus
MK211372
Coronavirus BtSk-
|
RsSHC014
AlphaCoV/GX2018D
|
KC881006
Bat SARS-like coronavirus
MK211373
Coronavirus BtRs-AlphaCoV/YN2018
|
Rs3367
|
KC962433
Transmissible gastroenteritis virus
MK211374
Coronavirus BtRl-BetaCoV/SC2018
|
isolate TGEV-HX
|
KF367457
Bat SARS-like coronavirus WIV1
MK211375
Coronavirus BtRs-BetaCoV/YN2018A
|
KF430219
Bat coronavirus
MK211376
Coronavirus BtRs-BetaCoV/YN2018B
|
CDPHE15/USA/2006
|
KF460437
Infectious bronchitis virus isolate
MK211377
Coronavirus BtRs-BetaCoV/YN2018C
|
VicS-v
|
KF530060
Human coronavirus OC43 strain
MK211378
Coronavirus BtRs-BetaCoV/YN2018D
|
OC43/human/USA/851-15/1985
|
KF530061
Human coronavirus OC43 strain
MK211379
Coronavirus BtRt-BetaCoV/GX2018
|
OC43/human/USA/901-43/1990
|
KF530063
Human coronavirus OC43 strain
MK217372
Infectious bronchitis virus strain
|
OC43/human/USA/9612-48/1996
I0221/17
|
KF530064
Human coronavirus OC43 strain
MK217373
Infectious bronchitis virus strain
|
OC43/human/USA/9612-9/1996
I0725/17
|
KF530065
Human coronavirus OC43 strain
MK217374
Infectious bronchitis virus strain
|
OC43/human/USA/901-41/1990
I0916/16
|
KF530066
Human coronavirus OC43 strain
MK217375
Infectious bronchitis virus strain
|
OC43/human/USA/901-33/1990
I1209/16
|
KF530067
Human coronavirus OC43 strain
MK303619
Human coronavirus OC43 isolate
|
OC43/human/USA/912-10/1991
MDS6
|
KF530068
Human coronavirus OC43 strain
MK303620
Human coronavirus OC43 isolate
|
OC43/human/USA/007-11/2000
MDS2
|
KF530069
Human coronavirus OC43 strain
MK303621
Human coronavirus OC43 isolate
|
OC43/human/USA/982-4/1998
MDS4
|
KF530070
Human coronavirus OC43 strain
MK303622
Human coronavirus OC43 isolate
|
OC43/human/USA/991-19/1999
MDS11
|
KF530071
Human coronavirus OC43 strain
MK303623
Human coronavirus OC43 isolate
|
OC43/human/USA/925-1/1992
MDS12
|
KF530072
Human coronavirus OC43 strain
MK303624
Human coronavirus OC43 isolate
|
OC43/human/USA/9712-13/1997
MDS14
|
KF530073
Human coronavirus OC43 strain
MK303625
Human coronavirus OC43 isolate
|
OC43/human/USA/8912-37/1989
MDS16
|
KF530074
Human coronavirus OC43 strain
MK309398
Infectious bronchitis virus isolate
|
OC43/human/USA/9212-33/1992
CK/CH/GD/HY16
|
KF530075
Human coronavirus OC43 strain
MK329221
Infectious bronchitis virus isolate CK-
|
OC43/human/USA/953-23/1995
CH-GX-YL17
|
KF530076
Human coronavirus OC43 strain
MK472067
Alphacoronavirus sp. isolate WA1087
|
OC43/human/USA/911-11/1991
|
KF530077
Human coronavirus OC43 strain
MK472068
Alphacoronavirus sp. isolate WA2028
|
OC43/human/USA/873-16/1987
|
KF530078
Human coronavirus OC43 strain
MK472069
Alphacoronavirus sp. isolate WA3301
|
OC43/human/USA/9612-29/1996
|
KF530079
Human coronavirus OC43 strain
MK472070
Alphacoronavirus sp. isolate WA3607
|
OC43/human/USA/913-29/1991
|
KF530080
Human coronavirus OC43 strain
MK472071
Alphacoronavirus sp. isolate WAAlc1
|
OC43/human/USA/9712-31/1997
|
KF530081
Human coronavirus OC43 strain
MK492263
Bat coronavirus strain BtCoV92
|
OC43/human/USA/991-5/1999
|
KF530082
Human coronavirus OC43 strain
MK574042
Infectious bronchitis virus strain
|
OC43/human/USA/912-11/1991
ck/CH/LHB/110615
|
KF530083
Human coronavirus OC43 strain
MK574043
Infectious bronchitis virus strain
|
OC43/human/USA/873-19/1987
ck/CH/LHB/110617
|
KF530084
Human coronavirus OC43 strain
MK581202
Infectious bronchitis virus strain
|
OC43/human/USA/951-18/1995
gammaCoV/Ck/Poland/80/1989
|
KF530085
Human coronavirus OC43 strain
MK581204
Infectious bronchitis virus strain
|
OC43/human/USA/871-25/1987
gammaCoV/Ck/Poland/255/1997
|
KF530086
Human coronavirus OC43 strain
MK720944
Tylonycteris bat coronavirus HKU33
|
OC43/human/USA/872-5/1987
strain GZ151867
|
KF530087
Human coronavirus OC43 strain
MK720945
Rhinolophus bat coronavirus HKU32
|
OC43/human/USA/873-6/1987
strain TLC26A
|
KF530088
Human coronavirus OC43 strain
MK720946
Rhinolophus bat coronavirus HKU32
|
OC43/human/USA/901-54/1990
strain TLC28A
|
KF530089
Human coronavirus OC43 strain
MK878536
Infectious bronchitis virus isolate
|
OC43/human/USA/911-66/1991
GA9977/2019
|
KF530090
Human coronavirus OC43 strain
MK907286
Erinaceus hedgehog coronavirus
|
OC43/human/USA/931-85/1993
HKU31 strain F6
|
KF530091
Human coronavirus OC43 strain
MK907287
Erinaceus hedgehog coronavirus
|
OC43/human/USA/911-58/1991
HKU31 strain Rs13
|
KF530092
Human coronavirus OC43 strain
MN026164
Human coronavirus OC43 isolate
|
OC43/human/USA/008-5/2000
OC43_KLF_01_2018
|
KF530094
Human coronavirus OC43 strain
MN096598
Infectious bronchitis virus strain
|
OC43/human/USA/912-36/1991
ck/CH/YNSL/160501
|
KF530095
Human coronavirus OC43 strain
MN165107
Feline coronavirus isolate XXN
|
OC43/human/USA/912-6/1991
|
KF530096
Human coronavirus OC43 strain
MN197549
Infectious bronchitis virus isolate
|
OC43/human/USA/911-38/1991
CK/CH/GD/GDTS13
|
KF530097
Human coronavirus OC43 strain
MN306036
Human coronavirus OC43 strain
|
OC43/human/USA/9211-43/1992
SC0682
|
KF530098
Human coronavirus OC43 strain
MN306041
Human coronavirus OC43 strain
|
OC43/human/USA/965-6/1996
SC0810
|
KF530099
Human coronavirus OC43 strain
MN306042
Human coronavirus OC43 strain
|
OC43/human/USA/971-5/1997
SC0839
|
KF530123
Feline coronavirus strain Felis
MN306043
Human coronavirus OC43 strain
|
catus/NLD/UU88/2010
SC0841
|
KF569996
Rhinolophus affinis coronavirus
MN306046
Human coronavirus 229E strain
|
isolate LYRa11
SC0865
|
KF636752
Bat Hp-
MN306053
Human coronavirus OC43 strain
|
betacoronavirus/Zhejiang2013
SC9430
|
KF663559
Infectious bronchitis virus isolate
MN310476
Human coronavirus OC43 strain
|
ck/CH/IBTZ/2012
SC9428
|
KF668605
Infectious bronchitis virus isolate
MN310478
Human coronavirus OC43 strain
|
CK/CH/SD09/005
SC0776
|
KF906249
Dromedary camel coronavirus
MN369046
Human coronavirus 229E strain
|
HKU23 strain HKU23-265F
SC9724
|
KF906250
Dromedary camel coronavirus
MN512434
Infectious bronchitis virus isolate
|
HKU23 strain HKU23-362F
IBV/Ck/Can/17-035614
|
KF923886
Human coronavirus OC43 isolate
MN512435
Infectious bronchitis virus isolate
|
1908A/2010
IBV/Ck/Can/17-036989
|
KF923887
Human coronavirus OC43 isolate
MN512436
Infectious bronchitis virus isolate
|
1997A/2010
IBV/Ck/Can/18-048192T
|
KF923888
Human coronavirus OC43 isolate
MN512437
Infectious bronchitis virus isolate
|
2145A/2010
IBV/Ck/Can/18-048430
|
KF923889
Human coronavirus OC43 isolate
MN512438
Infectious bronchitis virus isolate
|
1926/2006
IBV/Ck/Can/18-049707
|
KF923890
Human coronavirus OC43 isolate
MN514962
Dromedary camel coronavirus HKU23
|
39A/2007
isolate DcCoV-
|
HKU23/camel/Ethiopia/CAC1019/2015
|
KF923891
Human coronavirus OC43 isolate
MN514963
Dromedary camel coronavirus HKU23
|
5240/2007
isolate DcCoV-
|
HKU23/camel/Morocco/CAC2586/2016
|
KF923892
Human coronavirus OC43 isolate
MN514964
Dromedary camel coronavirus HKU23
|
5345/2007
isolate DcCoV-
|
HKU23/camel/Nigeria/NV1010/2015
|
KF923893
Human coronavirus OC43 isolate
MN514965
Dromedary camel coronavirus HKU23
|
2151A/2010
isolate DcCoV-
|
HKU23/camel/Nigeria/NV1092/2015
|
KF923894
Human coronavirus OC43 isolate
MN514966
Dromedary camel coronavirus HKU23
|
5352/2007
isolate DcCoV-
|
HKU23/camel/Nigeria/NV1097/2015
|
KF923895
Human coronavirus OC43 isolate
MN514967
Dromedary camel coronavirus HKU23
|
10285/2010
isolate DcCoV-
|
HKU23/camel/Nigeria/NV1385/2016
|
KF923896
Human coronavirus OC43 isolate
MN611517
Rousettus aegyptiacus bat coronavirus
|
3074A/2012
229E-related isolate 5425
|
KF923897
Human coronavirus OC43 isolate
MN611518
Miniopterus pusillus bat coronavirus
|
3269A/2012
HKU8-related isolate 6610
|
KF923898
Human coronavirus OC43 isolate
MN611521
Scotophilus kuhlii bat coronavirus 512-
|
3184A/2012
related isolate HK140714
|
KF923899
Human coronavirus OC43 isolate
MN611522
Rhinolophus affinis bat coronavirus
|
3582/2006
HKU2-related isolate 160660
|
KF923900
Human coronavirus OC43 isolate
MN611524
Miniopterus schreibersii bat
|
3647/2006
coronavirus 1-related isolate 161454
|
KF923901
Human coronavirus OC43 isolate
MN611525
Hipposideros pomona bat coronavirus
|
5472/2007
CHB25 isolate CHB0025
|
KF923902
Human coronavirus OC43 isolate
NC_003045
Bovine coronavirus
|
12689/2012
|
KF923903
Human coronavirus OC43 isolate
NC_038861
Transmissible gastroenteritis virus
|
12691/2012
complete genome
|
KF923904
Human coronavirus OC43 isolate
NC_045512
Wuhan seafood market pneumonia
|
12694/2012
virus isolate Wuhan-Hu-1
|
KF923905
Human coronavirus OC43 isolate
U00735
Bovine coronavirus strain Mebus
|
229/2005
|
KF923906
Human coronavirus OC43 isolate
PDF_0663
#N/A
|
3194A/2012
|
KF923907
Human coronavirus OC43 isolate
GCS_003
#N/A
|
5370/2007
|
KF923908
Human coronavirus OC43 isolate
GCS_019
#N/A
|
5414/2007
|
KF923909
Human coronavirus OC43 isolate
PDF_2560
#N/A
|
5442/2007
|
KF923910
Human coronavirus OC43 isolate
PDF_3316
#N/A
|
5445/2007
|
KF923911
Human coronavirus OC43 isolate
GCS_242
#N/A
|
5479/2007
|
KF923912
Human coronavirus OC43 isolate
GCS_264a
#N/A
|
5484/2007
|
KF923913
Human coronavirus OC43 isolate
GCS_264b
#N/A
|
5485/2007
|
KF923914
Human coronavirus OC43 isolate
GCS_265a
#N/A
|
5508/2007
|
KF923915
Human coronavirus OC43 isolate
GCS_265b
#N/A
|
5517/2007
|
KF923916
Human coronavirus OC43 isolate
SBB_122
#N/A
|
5519/2007
|
KF923917
Human coronavirus OC43 isolate
PDF_1704
#N/A
|
5566/2007
|
KF923918
Human coronavirus OC43 isolate
GCS_008
#N/A
|
10108/2010
|
KF923919
Human coronavirus OC43 isolate
SBB_943
#N/A
|
5595/2007
|
KF923920
Human coronavirus OC43 isolate
SBB_797
#N/A
|
5617/2007
|
KF923921
Human coronavirus OC43 isolate
PDF_2370
#N/A
|
69A/2007
|
KF923922
Human coronavirus OC43 isolate
PDF_2386
#N/A
|
8164/2009
|
KF923923
Human coronavirus OC43 isolate
GCS_017
#N/A
|
892A/2008
|
KF923924
Human coronavirus OC43 isolate
PRD_0038
#N/A
|
10290/2010
|
KF923925
Human coronavirus OC43 isolate
PDF_2180
#N/A
|
10574/2010
|
KF931628
Infectious bronchitis virus isolate
SBB_120
#N/A
|
VicS-del
|
KJ473795
BtMf-AlphaCoV/AH2011
SBB_041
#N/A
|
KJ473796
BtMf-AlphaCoV/JX2012
SBB_053
#N/A
|
KJ473797
BtMf-AlphaCoV/GD2012
SBB_010
#N/A
|
KJ473798
BtMf-AlphaCoV/HuB2013
SBB_024
#N/A
|
KJ473799
BtMf-AlphaCoV/FJ2012
SBB_726
#N/A
|
KJ473800
BtMf-AlphaCoV/HeN2013
SBB_747
#N/A
|
KJ473806
BtMr-AlphaCoV/SAX2011
SBB_788
#N/A
|
KJ473807
BtRf-AlphaCoV/HuB2013
SBB_130
#N/A
|
KJ473808
BtRf-AlphaCoV/YN2012
GCS_011
#N/A
|
KJ473809
BtNv-AlphaCoV/SC2013
GCS_211
#N/A
|
KJ473810
BtMs-AlphaCoV/GS2013
GCS_227
#N/A
|
KJ473811
BtRf-BetaCoV/JL2012
NC_009020
Pipistrellus bat coronavirus HKU5
|
KJ473812
BtRf-BetaCoV/HeB2013
EF065508
Bat coronavirus HKU4-4
|
KJ473813
BtRf-BetaCoV/SX2013
EF065505
Bat coronavirus HKU4-1
|
KJ473814
BtRs-BetaCoV/HuB2013
EF065506
Bat coronavirus HKU4-2
|
KJ473815
BtRs-BetaCoV/GX2013
EF065507
Bat coronavirus HKU4-3
|
KJ473816
BtRs-BetaCoV/YN2013
NC_009019
Tylonycteris bat coronavirus HKU4
|
KJ473821
BtVs-BetaCoV/SC2013
MH002339
Tylonycteris bat coronavirus HKU4
|
KJ958218
Human coronavirus OC43 strain
MH002337
Tylonycteris bat coronavirus HKU4
|
LY341
|
KJ958219
Human coronavirus OC43 strain
MH002338
Tylonycteris bat coronavirus HKU4
|
LY342
|
KM213963
Infectious bronchitis virus isolate
MW218395
Tylonycteris bat coronavirus HKU4
|
CK/CH/XDC- 2/2013
|
KM347965
Ferret coronavirus isolate FRCoV-
MN611519
Tylonycteris pachypus bat coronavirus
|
NL-2010
HKU4-related isolate GZ131656
|
KM454473
Duck coronavirus isolate
MH002342
Pipistrellus bat coronavirus HKU5
|
DK/GD/27/2014
|
KP143507
Feline coronavirus isolate 27C
MN611520
Pipistrellus abramus bat coronavirus
|
HKU5-related isolate BY140568
|
KP143508
Feline coronavirus isolate 28O
MH002340
Pipistrellus bat coronavirus HKU5
|
KP143509
Feline coronavirus isolate 65F
EF065510
Bat coronavirus HKU5-2
|
KP143510
Feline coronavirus isolate 67F
EF065511
Bat coronavirus HKU5-3
|
KP143511
Feline coronavirus isolate 80F
EF065512
Bat coronavirus HKU5-5
|
KP143512
Feline coronavirus isolate 26M
EF065509
Bat coronavirus HKU5-1
|
KP198610
Human coronavirus OC43 strain
MH002341
Pipistrellus bat coronavirus HKU5
|
2058A/10
|
KP198611
Human coronavirus OC43 strain
|
1783A/10
|
|
Example 2. RNA Preparation and Tagmentation Enrichment of RNAs of Interest in Wastewater Samples
RNA sequencing (RNA-Seq) with next-generation sequencing (NGS) is a powerful method for discovering, profiling, and quantifying RNA transcripts. Targeted RNA-Seq analyzes expression in a focused set of genes. Enrichment enables cost-effective RNA exome analysis using sequence-specific capture of the coding regions of the transcriptome. It is ideal for low-quality samples.
This tagmentation enrichment uses on-bead tagmentation followed by a single 90-minute hybridization step to provide a rapid workflow. On-bead tagmentation features enrichment Bead-Linked Transposomes (eBLT) optimized for RNA (eBLTL) that mediate a uniform tagmentation reaction. In addition to manual preparation, RNA Preparation and Tagmentation Enrichment is designed to be compatible with liquid-handling platforms for an automated workflow, providing highly reproducible sample handling, reduced risk of human error, and less hands-on time.
A. cDNA Synthesis and Tagmentation
Wastewater is collected for evaluation of viral RNA. RNA collected from wastewater is denatured and then random hexamers are annealed. The random hexamers prime the sample for cDNA synthesis. The hexamer-primed RNA fragments are then reverse transcribed to produce first strand cDNA. Enrichment Bead-Linked Transposomes are used to tagment double-stranded cDNA.
B. Amplification and Purification
After tagmentation, the fragments are purified and amplified to add index adapter sequences for dual indexing and P7 and P5 sequences for clustering. Next, magnetic beads are implemented to purify the tagmented library. Then the purified library is quantified and normalized.
C. Enrichment
After normalization, the library is combined into one pool for one- or three-plex enrichment. Results are optimized for 200 ng of each library. Following quantification and normalization, the magnetic beads are implemented to capture probes hybridized to the targeted library fragments of interest. Using heated washes, nonspecific binding is removed from the beads. The enriched library is then eluted from the beads. The enriched library is then amplified using a PCR program. In some embodiments, the PCR program is 14 cycles. After amplification, magnetic beads are used purify the enriched library.
D. Evaluation
The enriched library is then evaluated using either or both of the following methods: (1) analyzing 1 μl of the enriched library with the Qubit dsDNA HS Assay kit (Illumina) to quantify library concentration (ng/μl); and/or (2) analyzing 1 μl of the enriched library with the Agilent 2100 Bioanalyzer System and a DNA 1000 Kit to qualify.
After diluting to the starting concentration depending on the sequence system, libraries are denatured and diluted to the final loading concentration. Paired-end runs are used for sequencing. The number of cycles per index read is 10, and the number of cycles per read varies depending on the sequencing system.
Example 3. Enrichment Using a Solid Support
A solid support, such as a flowcell, is prepared for enrichment. Oligonucleotides are prepared corresponding to desired RNA, and these oligonucleotides are immobilized to a solid support. For example, oligonucleotides comprising sequences complementary to desired RNA (e.g., RNA sequences associated with coronaviruses) are immobilized to a solid support to allow for enrichment. A flowcell with such immobilized oligonucleotides may be termed an enrichment flowcell.
A cDNA library is prepared using the probe sets described above in Example 1 from a wastewater sample comprising RNA. Library fragments are then be added to the enrichment flowcell. Library fragments prepared from desired RNA bind to the enrichment flowcell, and the fluid that does not bind to the enrichment flowcell (comprising library fragments not prepared from desired RNA) is siphoned to a waste container. The bound library fragments are denatured, collected, and sequenced (with optional amplification before sequencing). In this way, the library that is sequenced is enriched for library fragments prepared from desired RNA.
Example 4. Pan-Coronavirus Panel for Genomic Surveillance of Coronaviruses Using Target Enrichment NGS
The performance of a panel comprising coronavirus enrichment probes described herein (Pan-CoV panel) was evaluated in an enrichment assay (Illumina RNA Prep with Enrichment or “IRPE”) followed by Illumina SBS sequencing using synthetic controls representative of four different coronaviruses. The Pan-CoV panel contains probes to >200 human and animal coronaviruses. Compared to untargeted sequencing, enrichment using the Pan-CoV panel provides much more depth of genome coverage and sensitivity (>100-fold enrichment at low viral loads). Mixing studies demonstrated that different controls of Coronaviruses can be detected together, revealing the robustness of this assay. In addition, the design strategy of Pan-CoV panel and IRPE chemistry can also tolerate a certain level of mismatches such that diverse coronaviruses sequences including new variants, as indicated by the identification of BA.2 variant of SARS-COV-2 that emerged after the panel development was complete.
To further demonstrate the applications of this assay in real-world samples, enrichment using the Pan-CoV panel was performed on 48 RNA samples extracted from bat feces collected from Africa followed by sequencing. When performing assembly analysis, it was found that enriched libraries showed more and longer contigs compared to untargeted sequencing. Alignment of these contigs to widely used databases showed similarity to currently characterized Bat and Human Coronaviruses but have significant divergence in regions of the genome, seemingly due to the novelty of the Coronaviruses sequenced. To provide a comprehensive analysis solution, a DRAGEN Microbial Enrichment App was developed, which provides users with consensus genomes and contigs, enabling novel coronaviruses discovery and characterization, and using these two methods together provides benefits. Thus, the panel comprising coronavirus enrichment probes is a powerful tool for monitoring the spread of coronaviruses to prevent the next pandemic.
EQUIVALENTS
The foregoing written specification is considered to be sufficient to enable one skilled in the art to practice the embodiments. The foregoing description and Examples detail certain embodiments and describes the best mode contemplated by the inventors. It will be appreciated, however, that no matter how detailed the foregoing may appear in text, the embodiment may be practiced in many ways and should be construed in accordance with the appended claims and any equivalents thereof.
As used herein, the term about refers to a numeric value, including, for example, whole numbers, fractions, and percentages, whether or not explicitly indicated. The term about generally refers to a range of numerical values (e.g., +/−5-10% of the recited range) that one of ordinary skill in the art would consider equivalent to the recited value (e.g., having the same function or result). When terms such as at least and about precede a list of numerical values or ranges, the terms modify all of the values or ranges provided in the list. In some instances, the term about may include numerical values that are rounded to the nearest significant figure.