Claims
- 1. A peptide nucleic acid probe comprising from 15 to 30 polymerized moieties of formula (I) wherein each X and Y are independently chosen from O and S, each Z independently is chosen from O, S, NR1, and C(R1)2, wherein each R1 is independently chosen from H, C1-6 alkyl, C1-6 alkenyl, and C1-6 alkynyl, each R2, R3 and R4 are independently chosen from H, the side chain of a naturally occurring amino acid, the side chain of a non-naturally occurring amino acid, C1-4 alkyl, C1-4 alkenyl, C1-4 alkynyl, and a functional group, and each Q is independently chosen from a naturally occurring nucleobase, a non-naturally occurring nucleobase, an intercalator, a nucleobase-binding group, a label and H, wherein a series of Qs of adjacent moieties are chosen so as to form one of the following subsequences GGC TTT TAA GGA TTC (SEQ ID NO: 40) GAT CAA TGC TCG GTT (SEQ ID NO: 44) CGA CTC CAC ACA AAC (SEQ ID NO: 76) GTC TTT TCG TCC TGC (SEQ ID NO: 89), or GTC TTA TCG TCC TGC (SEQ ID NO: 90) or a mixture of such probes.
- 2. The peptide nucleic acid probe of claim 1, wherein each of the 15 to 30 polymerized moieties of formula (I) are independently chosen from or a mixture of such probes.
- 3. The peptide nucleic acid probe according to claim 1, wherein each Z is independently chosen from NH, NCH3 and O, andeach R2, R3 and R4 is independently chosen from H, the side chain of a naturally occurring amino acid, the side chain of a non-naturally occurring amino acid, and C1-4 alkyl, or a mixture of such probes.
- 4. The peptide nucleic acid probe according to claim 1, wherein each Z is independently chosen from NH and O, andeach R2 is independently chosen from H or the side chain of Ala, Asp, Cys, Glu, His, HomoCys, Lys, Orn, Ser and Thr, or a mixture of such probes.
- 5. The peptide nucleic acid probe of claim 1, further comprising one or more labels, or a mixture of such probes, which labels may be mutually identical or different, which probes may comprise one or more linkers, and which probes may be mutually identical or different.
- 6. The peptide nucleic acid probe of claim 1 chosen fromLys(Flu)-GGC TTT TAA GGA TTC-NH2(OK 689/modified SEQID NO: 40)Lys(Rho)-GGC TTT TAA GGA TTC-NH2(OK 702/modified SEQID NO: 40)Flu-β-Ala-β-Ala-GAT CAA TGC TCG(OK 624/modified SEQGTT-NH2ID NO: 44)Flu-β-Ala-β-Ala-CGA CTC CAC ACA(OK 612/modified SEQAAC-NH2ID NO: 76)Lys(Flu)-GTC TTT TCG TCC TGC-NH2(OK 745/modified SEQID NO: 89)Lys(Rho)-GTC TTA TCG TCC TGC-NH2(OK 746/modified SEQID NO: 90)wherein Flu denotes a 5-(and 6)-carboxyfluoroescein label and Rho denotes a rhodamine label, or a mixture of such probes.
- 7. A kit comprising:at least one peptide nucleic acid probe comprising from 15 to 30 polymerized moieties of formula (I) wherein each X and Y are independently chosen from O and S, each Z independently is chosen from O, S, NR1, and C(R1)2, wherein each R1 is independently chosen from H, C1-6 alkyl, C1-6 alkenyl, and C1-6 alkynyl, each R2, R3 and R4 are independently chosen from H, the side chain of a naturally occurring amino acid, the side chain of a non-naturally occurring amino acid, C1-4 alkyl, C1-4 alkenyl, C1-4 alkynyl, and a functional group, and each Q is independently chosen from a naturally occurring nucleobase, a non-naturally occurring nucleobase, an intercalator, a nucleobase-binding group, a label or H, wherein a series of Qs of adjacent moieties are chosen so as to form one of the following subsequences GGC TTT TAA GGA TTC (SEQ ID NO: 40) GAT CAA TGC TCG GTT (SEQ ID NO: 44) CGA CTC CAC ACA AAC (SEQ ID NO: 76) GTC TTT TCG TCC TGC (SEQ ID NO: 89), or GTC TTA TCG TCC TGC (SEQ ID NO: 90) and a detection system with at least one detecting reagent.
- 8. The kit of claim 7 comprising a mixture of the at least one peptide nucleic acid probe.
- 9. The kit of claim 7, further comprising a solid phase capture system.
Parent Case Info
This a divisional of Ser. No. 08/943,777, filed Oct. 3, 1997 now abandoned.
The present application claims priority under 35 USC 119(e) (1) from Provisional Application Nos. 60/028,392 filed on Oct. 15, 1996, 60/029,595 filed on Oct. 23, 1996 and 60/045,962, filed on May 8, 1997.
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