Claims
- 1. Apparatus providing a closed system for the treatment of samples with a succession of liquids, such as the staining of biological samples, comprising:
- a closed chamber within which said samples are to be successively treated by said succession of liquids;
- said chamber including removable closure means for the insertion and removal of said samples;
- said chamber being provided with respective bores at the top and bottom thereof for the selective flow of fluid into the closed chamber through a respective one of said bores and the simultaneously outflow of fluid from the closed chamber through the respective other of said bores;
- a plurality of flexible-walled closed containers for supplying the respective liquids for treatment of said samples, each of said containers having an outlet for the discharge of contained liquid;
- flow controlling means including reversing valve means for selective connection with a respective one of the outlets of said flexible-walled containers and for selectively connecting said one outlet with one of the bores of the closed chamber to conduct the contained liquid into the chamber selectively at the top or the bottom of the chamber depending upon whether the ratio between the specific density of the succeeding liquid is higher or lower than the specific density of the preceding liquid, and;
- a source of vacuum having a pump and an inlet;
- said flow controlling means also including means for selectively connecting the inlet of the source of vacuum to the other bore of the closed chamber to induce flow of liquid from one of said containers into said chamber through said one bore from top to bottom or from bottom to top depending upon the density ratio between the succeeding and preceding liquids to flow through the chamber.
- 2. Apparatus of claim 1, wherein said flow controlling means include multiple shutoff valve means having a plurality of inlets for the respective sources of liquids all connected to a common outlet means connected, in turn, to said reversing valve means.
- 3. Apparatus of claim 2, wherein said shutoff valve means are connected to said outlet means in series, one of said liquids comprises a cleaning agent, and the shutoff valve means for said one liquid is connected farthest from the outlet means, whereby said cleaning agent will flow through all of the valve means when connected.
- 4. Process for the treatment of samples with a succession of liquids, such as the staining of biological samples comprising the steps:
- placing a sample to be treated into a closed chamber;
- placing each of the liquids into a separate closed supply vessel having a variable volume;
- filling each vessel with the selected one of said liquids and expelling gases therefrom;
- introducing a first liquid into said chamber from a supply vessel and filling the chamber completely to exclude gases therefrom;
- introducing a second liquid into said chamber from another supply vessel without introducing gas from the atmosphere and without mixing the first and second liquids while simultaneously withdrawing the liquid previously introduced into the chamber.
- 5. Process of claim 4, wherein a third liquid is introduced into said chamber from a supply vessel without introducing gas from the atmosphere while simultaneously withdrawing the second liquid from the chamber.
- 6. Process of claim 4, wherein the first liquid is withdrawn by subjecting it to a source of vacuum and the second liquid is introduced as a result of atmospheric pressure acting on said variable volume vessel.
- 7. Process of claim 6, wherein said variable volume vessel is a closed container having a collapsible wall.
- 8. Process of any one of claims 4, 5, 6 or 7, wherein said closed chamber is provided with a first bore at the top and a second bore at the bottom, and a succeeding light is introduced through one of the bores while the preceding liquid is withdrawn through the other bore, the succeeding liquid being introduced through the first bore if its specific density is less than that of the preceding light and being introduced through the second bore if its specific density is greater than that of the preceding liquid.
Priority Claims (1)
Number |
Date |
Country |
Kind |
7801564 |
Feb 1978 |
SEX |
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Parent Case Info
This is a continuation of application Ser. No. 10,846, filed Feb. 8, 1979, now abandoned.
US Referenced Citations (9)
Continuations (1)
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Number |
Date |
Country |
Parent |
10846 |
Feb 1979 |
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