Claims
- 1. A process for immobilizing an enzyme, comprising the steps of:
(a) selecting a supporting substrate; (b) activating the supporting substrate with an activating molecule to form an activated supporting substrate; (c) adding an enzyme and the activated supporting substrate to an organic solvent to form a mixture; and (d) obtaining an immobilized enzyme from the mixture, wherein the organic solvent contains from about 0.01% to about 30% by weight of the organic solvent of water.
- 2. The process according to claim 1, wherein the supporting substrate is an inorganic particle.
- 3. The process according to claim 2, wherein the supporting substrate has a particle size of from about 1 nanometer to about 10 micrometers.
- 4. The process according to claim 2, wherein the process further comprises the step of modifying the supporting substrate with a linking molecule after the selecting step.
- 5. The process according to claim 4, wherein the linking molecule is a silane linking molecule.
- 6. The process according to claim 1, wherein the activating molecule is a water soluble carbon diimide.
- 7. The process according to claim 1, wherein the enzyme is selected from the group consisting of a protease, an amylase, a lipase, a cellulase, a mannanase, a peroxidase or a mixture thereof.
- 8. The process according to claim 1, wherein the organic solvent is selected from the group consisting of hexane, toluene, a triglyceride, and a mixture thereof.
- 9. An immobilized enzyme which is immobilized by the process according to claim 1.
- 10. A cleaning composition comprising the immobilized enzyme according to claim 9.
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application claims the benefit of U.S. Provisional Application No. 60/402,280, filed Aug. 9, 2002.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60402280 |
Aug 2002 |
US |