Claims
- 1. Method for photometric determination of at least one of active protein C and active protein S in a sample comprising simultaneously incubating the sample with a protein C activator from snake venom which activates protein C and/or protein S, a chromogenic thrombin substrate and a coagulation system activator and determining cleavage of said chromogenic thrombin substrate by thrombin as an indicator of active protein C and/or protein S in said sample, without adding an inactivator of said protein C activator.
- 2. Method according to claim 1 wherein the protein C activator is a solution of venom of Agkistrodon contortrix contortrix or of components of said venom which activate protein C.
- 3. Method according to claim 1, wherein the protein C activator is venom of A. C. mokasen, A. C. pictigaster, A. piscivours, A. p. leucostoma, A. bilineatus, Bothrops moojeni, B. pradoi Cerastes cerrastes, Vipera lebetrina or V. russellii.
- 4. Method according to claim 1, wherein the chromogenic thrombin substrate used is H-D-Phe-Pip-Arg-pNA or Tos-Gly-Pro-Art-pNA.
- 5. Process according to claim 1 further comprising adding protein C deficient plasma to said sample.
- 6. Method according to claim 1 further comprising adding protein S deficient plasma to said sample.
- 7. Method of claim 1, wherein said sample is a plasma sample.
- 8. Method according to claim 1, further comprising adding an activator for factor XII to said sample.
- 9. Method according to claim 8 , wherein said factor XII activator is cephalin and ellagic acid.
- 10. Method according to claim 1 further comprising adding an activator for factor VII to said sample.
- 11. Method according to claim 10, wherein said factor VII activator is factor V and thromboplastin.
- 12. Method according to claim 1 further comprising adding an activator for factor II to said sample.
- 13. Method according to claim 12, wherein said factor II activator is cephalin and factor Xa.
Priority Claims (1)
Number |
Date |
Country |
Kind |
3607559 |
Mar 1986 |
DEX |
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Parent Case Info
This application is a continuation of application Ser. No. 016,283, filed Feb. 19, 1987, now abandoned.
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Date |
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4289498 |
Baughman et al. |
Sep 1981 |
|
4334018 |
Kirchhof |
Jun 1982 |
|
4692406 |
Becker et al. |
Sep 1987 |
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Number |
Date |
Country |
0076042 |
Mar 1982 |
EPX |
0182929 |
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EPX |
0107383 |
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EPX |
Continuations (1)
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Number |
Date |
Country |
Parent |
16285 |
Feb 1987 |
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